Rapid nucleic acid electrophoresis analysis method and system and storage medium

Abstract

The invention relates to a rapid nucleic acid electrophoresis analysis method and system and a storage medium, the method is based on a micro-fluidic chip pre-filled with gel and provided with a plurality of physical isolation independent flow channels, each flow channel is provided with an independent electrode and an identification code for storing flow channel state information, during analysis, the system reads the identification code and intelligently identifies a sample loading flow channel, and the sample loading flow channel is analyzed. An electric field is selectively applied, and active heat dissipation is started at the same time. According to the method and the system, 'sample adding for detection 'of nucleic acid electrophoresis is realized, the traditional analysis time of more than 30 minutes is shortened to about 5 minutes, and cross contamination among samples is fundamentally avoided. The flow channel state is managed through the identification code, the chip is supported to be used for several times and flexibly, and the single-time detection cost is remarkably reduced. The method integrates the advantages of no gel preparation, high throughput, rapidness, pollution prevention and intelligent management, and is suitable for various scenes needing rapid nucleic acid analysis.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, and in particular to a rapid nucleic acid electrophoresis analysis method, system, and storage medium. Background Technology

[0002] Nucleic acid electrophoresis is a core technology used in molecular biology, clinical diagnostics, and other fields to analyze the size, purity, and integrity of nucleic acid fragments. Currently, mainstream methods include agarose gel electrophoresis, pre-prepared gel electrophoresis, and automated capillary electrophoresis. Traditional slab electrophoresis requires on-site gel preparation, which is cumbersome, time-consuming, and the reproducibility of results depends on the operator's experience. Pre-prepared gels simplify the preparation process, but their parameters are fixed, lack flexibility, are inconvenient to store, and are costly. Automated capillary electrophoresis instruments are expensive, require complex fluid piping maintenance, and the sequential flow of samples in shared capillaries poses a risk of cross-contamination, making it difficult to simultaneously meet the demands for rapid, low-cost, high-throughput, and contamination-free detection. Therefore, there is an urgent need in this field for a nucleic acid electrophoresis analysis solution that is simple to operate, fast in analysis, cost-effective, and fundamentally eliminates cross-contamination. Summary of the Invention

[0003] The main objective of this invention is to provide a rapid nucleic acid electrophoresis analysis method, system, and storage medium. By using pre-encapsulated gel, independent flow channel physical isolation, identification code status management, and selective electric field application and active heat dissipation technology, the system enables users to quickly complete the analysis by simply adding samples, avoids cross-contamination between samples, and allows for flexible use of the chip in multiple applications.

[0004] To achieve the above objectives, the present invention provides a rapid nucleic acid electrophoresis analysis method, comprising the following steps: A microfluidic chip, the chip comprising multiple physically isolated electrophoretic channels pre-filled with gel medium, each electrophoretic channel having an independent electrode pair, and the chip surface having an identification code storing the state information of each electrophoretic channel; The nucleic acid sample to be tested is injected into the injection area of ​​at least one of the selected electrophoresis channels; The chip is placed into an analysis system, which reads the identification code to obtain the status information. Based on the acquired state information, the analysis system selectively applies a separation electric field only to the electrophoresis channels of the injected sample and actively dissipates heat from the chip during the electrophoresis process. Obtain the electrophoretic separation results of the nucleic acid sample to be tested within the electrophoresis channel.

[0005] Furthermore, the microfluidic chip includes 32 electrophoretic channels; wherein, The sample inlet volume of each electrophoresis channel is 30 μL to 50 μL; The depth of the separation zone in each of the electrophoretic channels is 1.5 mm to 2.0 mm; Each electrode pair at both ends of the electrophoresis channel includes a positive electrode and a negative electrode, wherein the effective surface area of ​​the positive electrode in contact with the gel medium is greater than the effective surface area of ​​the negative electrode in contact with the gel medium.

[0006] Furthermore, the effective surface area of ​​the positive electrode is more than 1.5 times that of the effective surface area of ​​the negative electrode.

[0007] Furthermore, based on the acquired state information, the analysis system selectively applies a separation electric field only to the electrophoretic channels of the injected sample, including: If the status information indicates that an electrophoresis channel is in an "unused" state and the electrophoresis channel has been injected with a sample, then a separation electric field with a constant voltage of 100V to 150V is applied to the electrophoresis channel, and the electrophoresis time is 3 minutes to 8 minutes.

[0008] Furthermore, the rapid nucleic acid electrophoresis analysis method also includes: After obtaining the electrophoretic separation results, the analysis system updates the status information in the identification code corresponding to the used electrophoretic channel to "occupied".

[0009] The present invention also provides a rapid nucleic acid electrophoresis analysis system for performing the rapid nucleic acid electrophoresis analysis method described in any one of the above claims, characterized in that the system comprises: Chip carrier module, used to carry microfluidic chips; The identification module is used to read the identification code on the surface of the chip to obtain the status information of each electrophoretic channel; The control module, which is communicatively connected to the identification module, is used to generate control commands based on the status information; An electric field application module, connected to the control module, is used to selectively apply a separation electric field only to the electrophoretic channels of the injected sample on the chip according to the control command. A heat dissipation module, connected to the control module, is used to actively dissipate heat from the chip during the electrophoresis process; The detection module is used to obtain the electrophoretic separation results of the samples within the chip.

[0010] Furthermore, the control module is also used to generate a status update instruction after the detection module obtains the separation result; the identification module is also used to update the status information in the identification code corresponding to the used electrophoresis channel according to the status update instruction.

[0011] Furthermore, the electric field application module includes multiple independent electrode driving units, each of which is individually connected to an electrode pair of an electrophoretic channel on the chip and can be controlled to switch on and off.

[0012] Furthermore, the heat dissipation module is a semiconductor cooling module, and its cooling surface is configured to be in close contact with the cover surface of the chip.

[0013] The present invention also provides a computer-readable storage medium having a computer program stored thereon, wherein when the computer program is executed by a processor, it implements the rapid nucleic acid electrophoresis analysis method described in any of the above claims.

[0014] This invention provides a rapid nucleic acid electrophoresis analysis method, system, and storage medium, which has the following beneficial effects: By pre-encapsulating gel within the chip, it achieves extremely simple operation—sample addition followed by testing—completely eliminating the cumbersome process of traditional gel preparation, reducing reliance on operator experience, and making the detection highly standardized. The optimized flow channel size, combined with an active cooling system, significantly reduces the nucleic acid electrophoresis analysis time from over 30 minutes to approximately 5 minutes, achieving an order-of-magnitude improvement in speed. Simultaneously, physically isolated independent flow channels fundamentally eliminate cross-contamination between samples, ensuring absolute reliability of the results. Furthermore, combined with intelligent status management using identification codes, users can flexibly select and use flow channels multiple times, significantly improving chip utilization and reducing the cost per test. Finally, the system adopts an integrated design, eliminating the need for complex fluid piping and vulnerable components, fundamentally avoiding malfunctions such as blockages and leaks, greatly improving the long-term stability and reliability of the equipment, and making maintenance extremely simple. Attached Figure Description

[0015] Figure 1 This is a flowchart illustrating a rapid nucleic acid electrophoresis analysis method according to an embodiment of the present invention; Figure 2 This is a structural block diagram of a rapid nucleic acid electrophoresis analysis device according to an embodiment of the present invention; The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0017] Reference Figure 1 The diagram below illustrates the flowchart of a rapid nucleic acid electrophoresis analysis method proposed in this invention, which includes the following steps: S1, the chip includes multiple physically isolated electrophoretic channels pre-filled with gel medium, each electrophoretic channel is provided with an independent electrode pair, and the chip surface is provided with an identification code storing the status information of each electrophoretic channel; S2, inject the nucleic acid sample to be tested into the injection area of ​​at least one of the selected electrophoresis channels; S3, the chip is placed into an analysis system, and the analysis system reads the identification code to obtain the status information; S4, the analysis system selectively applies a separation electric field only to the electrophoresis channel of the injected sample based on the acquired state information, and actively dissipates heat from the chip during the electrophoresis process; S5, obtain the electrophoretic separation result of the nucleic acid sample to be tested in the electrophoresis channel.

[0018] As described in step S1 above, the chip includes multiple physically isolated electrophoretic channels pre-filled with gel medium. Each electrophoretic channel is provided with an independent electrode pair. The chip surface is provided with an identification code storing the state information of each electrophoretic channel. The microfluidic chip has multiple physically isolated electrophoretic channels. The channels are pre-filled and solidified with gel separation media such as agarose or polyacrylamide. Each electrophoretic channel has an independent electrode pair integrated at both ends. In addition, the chip surface is provided with an identification code that includes a QR code and data storage function. The identification code is pre-written or associated with the initial state information of each electrophoretic channel in the chip, such as "unused" or "used".

[0019] As described in step S2 above, the chip is placed into an analysis system. The analysis system reads the identification code to obtain the status information, thereby realizing chip loading and information recognition. The chip is placed into the chip carrier position dedicated to the analysis system in a preset orientation. After the chip is in place, the identification module of the analysis system automatically reads the identification code on the chip surface and transmits the decoded information to the system's control module. The control module then obtains and loads the current status information table of all electrophoretic channels of the chip, completing the system's initial recognition of the current chip operating status.

[0020] As described in step S3 above, the analysis system determines the target electrophoresis channel for sample loading according to the predetermined detection program or user instructions. Subsequently, the system's automated liquid handling module moves to the sample source, aspirates a predetermined volume of nucleic acid sample (e.g., 1-3 μL), and then the pipette tip is precisely positioned and lowered above the injection area corresponding to the target electrophoresis channel, injecting the sample solution into the injection area. Under the influence of gravity and fluid, the sample enters the gel region at the front end of the channel through the channel at the bottom of the injection area. This automated sample loading process ensures the accuracy of the sample volume and the consistency of the loading position, eliminating errors caused by manual operation.

[0021] As described in step S4 above, the analysis system, based on the acquired status information, selectively applies a separation electric field only to the electrophoresis channels where samples have been injected, and actively dissipates heat from the chip during electrophoresis. The system's control module, based on the status information acquired in the preceding steps, determines whether to issue a start command only to the electrophoresis channels whose status is "unused" and which have already undergone automated sample loading in step S3. The electric field application module within the system responds to the command, controlling the corresponding independent electrode driving unit to apply a constant separation electric field with a preset voltage value within the range of 100V to 150V to the electrode pairs of that channel. Simultaneously, the control module activates the heat dissipation module, ensuring that its effective cooling surface is in close contact with the chip body, thereby actively dissipating heat from the chip throughout the electrophoresis process and effectively removing Joule heat.

[0022] As described in step S5 above, after the preset electrophoresis time is reached, the system automatically terminates the electric field. Subsequently, the integrated optical detection module, such as a fluorescence imaging system or a staining imaging system, is triggered to scan the separation regions of all channels on the chip, capturing images of the nucleic acid band distribution. Finally, the system software analyzes and processes the images, automatically calculating the size, concentration, and other information of the nucleic acid fragment to be tested by comparing it with a standard reference, and generates a formatted detection report.

[0023] In one embodiment, the chip includes multiple physically isolated electrophoretic channels pre-filled with gel medium, each electrophoretic channel having an independent electrode pair, and step S1, in which the chip surface has an identification code storing the state information of each electrophoretic channel, includes: S11, System Readiness and Physical Chip Loading; S12, chip identification and digital acquisition of status information; S13, System resource configuration and process readiness determination.

[0024] In the specific implementation process, the operator removes one of the microfluidic chips from the packaging and smoothly pushes it into the carrier chamber along the guide groove until it touches the bottom. This immediately triggers the high-precision position sensor at the bottom of the carrier chamber, which sends a high-level "chip in place" confirmation signal to the system's control module. This step signifies that the physical chip has been physically accepted by the system. Upon receiving the "chip in place" signal, the control module activates the QR code scanner integrated inside the door within 50 milliseconds. The positioning laser emitted by the scanner automatically aligns with the QR code recognition area on the chip surface, completing rapid imaging and transmitting the image data to the decoding unit. The decoding unit parses the information contained in the QR code to reconstruct a structured data packet.

[0025] The core content of this data packet includes: a unique chip ID, production parameters, and a 32-bit status data string, where each bit corresponds to an electrophoresis channel, with '0' representing "unused" and '1' representing "occupied". The control module loads the status data string from this data packet into its allocated temporary memory area, forming a "channel status mapping table" specifically for this experiment. After the system completes the "digital cloning" of the physical chip, it accurately grasps the availability of each independent channel. Based on the loaded "channel status mapping table", the control module performs resource matching and initialization operations. First, the control module verifies the chip specifications, such as determining the compatibility between the channel chip and the system configuration through the unique ID prefix.

[0026] Next, the system software interface refreshes synchronously, displaying the mapping table in a 32-square grid: all '0' states, representing unused channels, are displayed in green and are available. Simultaneously, the control module queries the electric field application module for the availability of its 32 independent electrode drive units and sends a pre-start command to the heat dissipation module, initiating pre-cooling of its semiconductor cooler to the base temperature. Once the interface refresh is complete, all hardware modules report "ready," and the control module confirms the "channel status mapping table" has been effectively established, the system displays "Chip ready, sampling can begin." This step signifies that the system has completed the allocation and preparation of all hardware and software resources based on the digital status of this specific chip.

[0027] In one embodiment, step S2, which involves injecting a nucleic acid sample to be tested into the injection region of at least one selected electrophoresis channel, includes: S21, Task Analysis and Sampling Path Planning; S22, Sample collection and precise positioning; S23, Perform sample addition and provide real-time feedback; S24, Status Update and Process Progression.

[0028] In practice, when the user clicks "Start Running" on the system software interface or imports a sample table containing sample information, the control module parses the instructions. First, it checks the internal "channel status mapping table" and automatically filters out all channels with a status of "unused" as candidate positions. The control module then adds samples A, B, and C to channels 1, 5, and 9 respectively according to the instructions, planning the movement path of the liquid processing module. The liquid processing module typically consists of a high-precision three-dimensional robotic arm and its onboard sample dispensing tip. The robotic arm first drives the sample dispensing tip to the sample source position. The tip descends and, through its internal high-precision piston or pressure sensor, accurately aspirates a preset volume of 1.5 μL of the nucleic acid sample to be tested. After aspiration, the robotic arm rises and quickly moves above the microfluidic chip along the planned optimal path. The sample dispensing tip is ultimately precisely positioned directly above the cylindrical injection area corresponding to the target electrophoresis channel. The pipette tip descends smoothly to a preset height above the liquid surface in the injection zone, then ejects the sample stream at a set flow rate. Under the influence of gravity and surface tension, the sample is completely injected into the injection zone. The system monitors the flow state using pressure or capacitance sensors integrated into the pipette tip to ensure accurate dispensing and detect potential blockages.

[0029] Once the sample dispensing is complete and the sensor feedback is normal, the dispensing nozzle is raised and moved away. After receiving a confirmation signal that the sample dispensing was successful, the control module immediately updates the internal "flow channel status mapping table," temporarily changing the status of the target flow channel that just completed the dispensing from "unused" to "sample loaded" or a similar relay status. At the same time, the system software interface is updated synchronously, changing the visual identifier of the flow channel, i.e., the square color, to yellow, to intuitively indicate "sample dispensing complete, ready to run." Once all the predetermined flow channels have completed the dispensing, the system automatically or waits for user confirmation to trigger the process to proceed to the next sequential step.

[0030] In one embodiment, step S3, in which the chip is placed into an analysis system and the analysis system reads the identification code to obtain the status information, includes: S31, chip transfer and system intervention; S32, precise chip positioning and multi-functional interface connection; S33, Secondary reading of the identification code and final verification of the status; S34, Electrophoresis parameters configured and system finally ready.

[0031] In the specific implementation process, after step S2 is completed and the system interface prompts "sample addition complete", the tray or robotic arm carrying the sampled chip begins to move smoothly from the "liquid processing area" or "sample addition position" to the analysis position inside the "electrophoresis detection chamber" of the instrument. The entire transfer process is carried out in a closed or controlled environment to prevent contamination or sample evaporation. When the chip reaches the predetermined analysis position, a high-precision micro switch or photoelectric sensor will be triggered to send a signal "chip has reached the analysis position" to the control module. The chip is secured within the testing chamber by precision fixtures or guiding mechanisms, ensuring highly repeatable spatial positioning. Next, the system executes two crucial physical connections: an electrical connection, where the corresponding electrode contact array within the testing chamber is pushed upwards by a micro-motor, achieving precise physical contact and electrical connection with the electrode sockets on the chip's edge, preparing the independent electrode pairs for each flow channel to connect to a controllable power supply; and a thermal connection, where the cooling surface of the semiconductor cooling module located on the testing chamber's upper cover descends smoothly under the action of a cylinder, achieving a large-area, uniform, and tight fit with the entire upper surface of the chip cover, ensuring an efficient heat conduction path. After the chip is securely fixed and the interface connections are complete, the control module initiates the final identification process. A backup or primary identification module located within the testing chamber quickly reads the QR code on the chip again to verify chip consistency and prevent accidental chip replacement during transfer; and obtains the final status baseline. The control module compares and synchronizes the original factory state stored in the read QR code with the updated channel state mapping table in memory from the previous steps. For example, it confirms which channels are marked as loaded with samples in S2 and formally defines the state of these channels in the upcoming electrophoresis process as "ready to run". Based on the final verified "channel state mapping table", the control module performs the final configuration before electrophoresis. According to the experimental plan, it sets electrophoresis parameters uniformly or individually for all "ready to run" channels, including a separation electric field voltage of 120V, a maximum electrophoresis duration of 8 minutes, and a target temperature of 20°C for the heat dissipation module. At the same time, the electric field application module reports that the connection of each electrode drive unit is normal, the heat dissipation module reports that the preheating temperature has been reached, and the detection module completes self-test and focuses.

[0032] Once all modules report "ready" to the control module, and the control module confirms that the parameter configuration has been loaded, the system marks the "to be run" flow channel as a flashing "ready" state on the software interface and prompts "System ready, electrophoresis can begin." After step S3 is completed, the system is in a critical state to execute step S4, which involves selectively applying the electric field and dissipating heat.

[0033] In one embodiment, step S4, in which the analysis system selectively applies a separation electric field only to the electrophoresis channels of the injected sample based on the acquired state information, and actively dissipates heat from the chip during electrophoresis, includes: S41, Selective electric field triggering and initialization; S42, Real-time process monitoring and dynamic adjustment; S43, multi-module collaboration and heat dissipation assurance; S44, Endpoint Judgment and Smooth Termination.

[0034] In practice, when the user clicks the "Start Electrophoresis" button on the software interface or the system triggers it automatically, the control module first sends an instruction containing the "target flow channel list" to the electric field application module.

[0035] This list, derived from the final verified "flow channel status mapping table," only contains the flow channel numbers with a status of "pending operation," such as 1, 5, and 9. Upon receiving the command, the electric field application module activates its corresponding independent electrode drive unit. These drive units operate synchronously, applying a preset constant DC voltage, such as 120V, to the plate-like platinum positive and negative electrodes of the target flow channel. Electrode drive units corresponding to non-target flow channels remain closed, resulting in no voltage difference between their electrode pairs and achieving "on-demand energy distribution." After electrophoresis is initiated, the system enters a closed-loop monitoring state. On one hand, the electric field application module continuously monitors the current value of each activated loop. If an abnormal surge in current is detected in a certain loop, it may indicate a short circuit or air bubble causing a sharp drop in resistance. The control module can cut off the electric field of that channel individually within 10 milliseconds and issue an alarm at the interface, while protecting other channels from being affected. On the other hand, the temperature sensor integrated in the detection chamber monitors the temperature of the chip cover surface and interior in real time. The control module compares this temperature feedback with a 20°C setpoint and dynamically adjusts the drive current of the heat dissipation module-semiconductor cooling module to achieve precise temperature control of ±0.5°C, suppressing the gel temperature rise and nucleic acid diffusion caused by electrophoresis Joule heating. Throughout the electrophoresis process, the operation of the heat dissipation module is strongly coupled with the applied electric field and real-time temperature. When the system applies high voltage to multiple channels simultaneously, the heat generation power increases. The control module will increase the cooling power based on the thermodynamic model feedforward. At the same time, the imaging system, as the detection module, performs a rapid preview scan at a low frequency of every 30 seconds, and the small amount of heat generated is also incorporated into the thermal management model. Multi-variable control of electric field, heat generation, heat dissipation, and monitoring ensures that the temperature of the chip's core working area can be effectively suppressed even under full-load operation of 32 flow channels, achieving "5-minute rapid electrophoresis" and obtaining clear bands. After the electrophoresis time enters the preset window, the control module instructs the detection module to perform a high-resolution scan, acquiring real-time images of the separation zones of all activated flow channels. The image analysis algorithm automatically determines whether each flow channel has reached the separation endpoint based on preset criteria indicating the arrival of the dye front at the end of the separation zone or user-defined timed termination conditions. Once a flow channel reaches the endpoint, the control module instructs the electric field application module to shut down the electrode drive unit corresponding to that flow channel. After all flow channels have reached the endpoint or reached the maximum safety time limit, the system uniformly shuts down all electric fields and controls the heat dissipation module to enter a heat preservation or slow recovery mode to prevent condensation. The system interface updates to "Electrophoresis Completed".

[0036] In one embodiment, step S5, which involves obtaining the electrophoretic separation result of the nucleic acid sample to be tested within the electrophoresis channel, includes: S51, high-resolution image data acquisition; S52, automated image processing and result calculation; S53, structured report generation and final chip status archiving.

[0037] In practice, once the control module confirms that all electrophoretic fields are off, it immediately sends a data acquisition command to the detection module. The LED excitation source of the imaging system illuminates at a preset wavelength and intensity, while the camera lens focuses on the focal plane containing all separation regions of the chip. The system performs a full-area scan, capturing a high-resolution fluorescence or staining image containing all 32 channels within seconds. This image clearly records the position, width, and fluorescence intensity information of the nucleic acid bands in each channel. To ensure data quality, the system may automatically perform background subtraction and flattening correction. The acquired raw image is transmitted to the control module or its connected dedicated analysis software. The software first automatically identifies the region of each independent channel in the image and, based on a pre-loaded "channel status mapping table," analyzes only the channels whose status is "running."

[0038] The analysis process includes: band detection, where the algorithm identifies significant band peaks along the migration direction of each channel; migration distance measurement, which accurately calculates the distance of the leading edge of each band peak relative to the starting gel sample well; and standard curve fitting and calculation, where the known molecular weight and migration distance of DNA standards running simultaneously in channel 16 or other user-specified channels are used to fit a standard curve. Using this curve, the software converts the migration distance of the sample bands to an accurate molecular weight. Simultaneously, based on the integral value of the band fluorescence intensity, the nucleic acid concentration can be relatively or absolutely quantified.

[0039] After all analysis and calculations are completed, the system automatically generates a structured test report. The report typically includes: chip ID, date and time, original electrophoretic patterns of each analytical channel, a table of band molecular weight and concentration data, and qualitative conclusions based on preset rules such as "pass / fail" or "size meets / does not meet". At the same time, to achieve intelligent traceability and anti-reuse of the chip, the control module executes a final status update instruction. This instruction drives the RFID writer integrated in the instrument to permanently update the status of all channels in the "channel status mapping table" used in this experiment that are not "unused" to "occupied" in the storage medium associated with the chip's QR code.

[0040] For example, if channels 1, 5, and 9 are used this time, the status bits corresponding to these three channels will be permanently locked to '1'. After this operation, if the chip is reinstalled into the system, the system will be able to immediately identify that these channels are unusable during the identification stage of step S1, thereby forcing the single-use or strict multi-use management of consumables and ensuring the reliability and compliance of the test.

[0041] In summary, this invention automatically acquires and establishes a digital state mapping for all flow channels by reading the chip's QR code. The system drives a robotic arm to perform precise, automated sample loading and updates the internal state table in real time. Based on this state information, during the electrophoresis stage, the system intelligently selects to apply a separation electric field only to the loaded flow channels, while simultaneously activating a semiconductor cooling module to actively dissipate heat from the chip throughout the process, achieving rapid and stable nucleic acid separation. After electrophoresis, the system automatically images the sample, analyzes the fragment size and concentration data using software, and generates a detection report instantly. Finally, the system permanently writes the flow channel state information used in this study into the chip's QR code, completing the chip's full lifecycle management. The entire process achieves unmanned operation from "chip identification" to "report output," combining the advantages of high throughput, contamination prevention, speed, and traceability.

[0042] In this embodiment, the specific implementation of each unit in the above device embodiment is described in the above method embodiment, and will not be repeated here.

[0043] Those skilled in the art will understand that all or part of the processes in the above embodiments can be implemented by a computer program instructing related hardware. The computer program can be stored in a non-volatile computer-readable storage medium. When executed, the computer program can include the processes of the embodiments of the above methods. Any references to memory, storage, databases, or other media used in the present invention and embodiments can include non-volatile and / or volatile memory. Non-volatile memory can include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory can include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in various forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), dual-rate SDRAM (SSRSDRAM), enhanced SDRAM (ESDRAM), synchronous link DRAM (SLDRAM), Rambus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM, etc.

[0044] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, apparatus, article, or method that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, apparatus, article, or method. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, apparatus, article, or method that includes that element.

[0045] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A rapid nucleic acid electrophoresis analysis method characterized by, Includes the following steps: A microfluidic chip, the chip comprising multiple physically isolated electrophoretic channels pre-filled with gel medium, each electrophoretic channel having an independent electrode pair, and the chip surface having an identification code storing the state information of each electrophoretic channel; The nucleic acid sample to be tested is injected into the injection area of ​​at least one of the selected electrophoresis channels; The chip is placed into an analysis system, which reads the identification code to obtain the status information. Based on the acquired state information, the analysis system selectively applies a separation electric field only to the electrophoresis channels of the injected sample and actively dissipates heat from the chip during the electrophoresis process. Obtain the electrophoretic separation results of the nucleic acid sample to be tested within the electrophoresis channel.

2. The rapid nucleic acid electrophoresis analysis method according to claim 1, characterized in that, The microfluidic chip includes 32 electrophoretic channels; wherein... The sample inlet volume of each electrophoresis channel is 30 μL to 50 μL; The depth of the separation zone in each of the electrophoretic channels is 1.5 mm to 2.0 mm; Each electrode pair at both ends of the electrophoresis channel includes a positive electrode and a negative electrode, wherein the effective surface area of ​​the positive electrode in contact with the gel medium is greater than the effective surface area of ​​the negative electrode in contact with the gel medium.

3. The rapid nucleic acid electrophoresis analysis method according to claim 2, characterized in that, The effective surface area of ​​the positive electrode is more than 1.5 times that of the effective surface area of ​​the negative electrode.

4. The rapid nucleic acid electrophoresis analysis method according to claim 1, characterized in that, Based on the acquired state information, the analysis system selectively applies a separation electric field only to the electrophoretic channels of the injected sample, including: If the status information indicates that an electrophoresis channel is in an "unused" state and the electrophoresis channel has been injected with a sample, then a separation electric field with a constant voltage of 100V to 150V is applied to the electrophoresis channel, and the electrophoresis time is 3 minutes to 8 minutes.

5. The rapid nucleic acid electrophoresis analysis method according to claim 1, characterized in that, The method further includes: After obtaining the electrophoretic separation results, the analysis system updates the status information in the identification code corresponding to the used electrophoretic channel to "occupied".

6. A rapid nucleic acid electrophoresis analysis system for performing the rapid nucleic acid electrophoresis analysis method according to any one of claims 1 to 5, characterized in that, The system includes: Chip carrier module, used to carry microfluidic chips; The identification module is used to read the identification code on the surface of the chip to obtain the status information of each electrophoretic channel; The control module, which is communicatively connected to the identification module, is used to generate control commands based on the status information; An electric field application module, connected to the control module, is used to selectively apply a separation electric field only to the electrophoretic channels of the injected sample on the chip according to the control command. A heat dissipation module, connected to the control module, is used to actively dissipate heat from the chip during electrophoresis. The detection module is used to obtain the electrophoretic separation results of the samples within the chip.

7. The rapid nucleic acid electrophoresis analysis system according to claim 6, characterized in that, The control module is further configured to generate a status update instruction after the detection module obtains the separation result; the identification module is further configured to update the status information in the identification code corresponding to the used electrophoresis channel according to the status update instruction.

8. The rapid nucleic acid electrophoresis analysis system according to claim 6, characterized in that, The electric field application module includes multiple independent electrode driving units, each of which is individually connected to an electrode pair of an electrophoretic channel on the chip and can be controlled to switch on and off.

9. The rapid nucleic acid electrophoresis analysis system according to claim 6, characterized in that, The heat dissipation module is a semiconductor cooling module, and its cooling surface is configured to be in close contact with the cover surface of the chip.

10. A computer-readable storage medium having a computer program stored thereon, characterized in that, When the computer program is executed by the processor, it implements the rapid nucleic acid electrophoresis analysis method as described in any one of claims 1 to 5.

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