Sample detection apparatus

Abstract

The invention discloses sample detection equipment. A sample box module comprises a sample detection box in which a plurality of reaction tubes are placed; the pushing module is used for driving the sample detection box to do linear motion, so that the sample detection box moves from the loading position to the detection position; the extraction module is mounted on the pushing module and is used for transferring a sample and a reagent into the reaction tube for uniform mixing; the detection module is mounted at the end part of the pushing module and is located at the detection position; the detection module is used for performing amplification and optical detection analysis on a sample in the reaction tube; the ultrasonic magnetic suction module is used for performing ultrasonic treatment on the liquid in the reaction tube. The method comprises the following steps: splitting and purifying a to-be-detected target sample through an extraction module to obtain sample nucleic acid; the detection module carries out same or different nucleic acid amplification detection on each reaction tube, optical detection is matched, mutual influence of different reaction tubes in the nucleic acid amplification detection process is avoided, multiple target objects are detected at a time, and the detection efficiency is improved.

Description

Sample testing equipment Technical Field

[0001] This application belongs to the field of sample testing technology, and specifically relates to a sample testing device. Background Technology

[0002] Rapid and accurate pathogen detection is a crucial step in the prevention and treatment of infectious diseases; therefore, pathogen nucleic acid testing plays an indispensable role in pathogen diagnosis. Infectious diseases progress rapidly and are highly variable, and the same symptoms can be caused by different pathogens. While high-throughput sequencing technology can detect multiple pathogens simultaneously, it has a high technical threshold, complex bioinformatics analysis and report interpretation, long processing times, and high costs, making it unsuitable for widespread testing. Therefore, pathogens are typically tested individually; however, this is labor-intensive, time-consuming, and prone to delaying diagnosis, resulting in low testing efficiency. Summary of the Invention

[0003] The purpose of this application is to provide a sample testing device to improve testing efficiency.

[0004] To achieve the above objectives, this application provides a sample testing device, including a frame and a component disposed within the frame:

[0005] The sample box module includes a sample detection box containing multiple reaction tubes;

[0006] The push module is used to drive the sample detection box to perform linear movement, so that the sample detection box moves from the loading position to the detection position.

[0007] An extraction module, installed on the push module, is used to transfer samples and reagents into reaction tubes for mixing.

[0008] The detection module, installed at the end of the push module and located at the detection position, is used to amplify and perform optical detection analysis on the sample in the reaction tube.

[0009] The ultrasonic magnetic suction module is used to ultrasonically treat the liquid inside the reaction tube.

[0010] In some embodiments, the sample box module further includes a loading seat driven and connected to the push module, the push module being used to drive the loading seat to move linearly from the loading position to the detection position, the sample detection box being placed inside the loading seat and including:

[0011] The first cover has a pipetting hole;

[0012] The bottom box is rotatably disposed inside the first cover. The bottom box forms a detection area and a liquid storage area. The liquid storage area is used to store samples and reagents for pretreatment of the samples. The detection area has multiple placement holes for placing reaction tubes. The liquid storage area and the multiple placement holes are arranged in a circumferential interval and can be rotated sequentially to communicate with the pipetting hole.

[0013] In some embodiments, the bottom box includes a panel and a surrounding panel, the surrounding panel surrounding the periphery of the panel, a placement groove and a liquid storage area are formed on the side of the panel facing the pipette hole, the side wall of the placement groove forms a detection area, and the placement hole is formed on the bottom wall of the placement groove.

[0014] In some embodiments, the panel has a connection hole, a placement slot and a liquid storage area are arranged around the outer periphery of the connection hole, and the sample detection box also includes a connection post, one end of which is rotatably passed through the connection hole and connected to the panel, and the other end of which is connected to the first cover.

[0015] In some embodiments, the frame includes a side plate with a guide groove, the guide groove having a laterally extending push guide section and a vertically extending lifting guide section, and the push module includes:

[0016] The connecting plate is connected to the sample box module at one end and has a sliding rod at the other end, which can slide through the guide groove.

[0017] The push drive component is used to drive the sliding rod to slide from one end of the push guide section to the lower end of the lifting guide section, so that the sliding rod drives the sample box module to move from the loading position to the detection position through the connecting plate.

[0018] In some embodiments, the push drive assembly includes a rocker arm and a push drive member. The rocker arm is oscillatingly mounted on a side plate and has a groove. A sliding rod can slide through the groove and extend into a guide groove. The push drive member is used to drive the rocker arm to oscillate, so that the rocker arm drives the sliding rod to slide from one end of the push guide section to the lower end of the lifting guide section.

[0019] In some embodiments, the push module further includes a guide component, which includes a push movable plate and a lifting movable plate. The push movable plate is disposed on the sample box module, and a first guide rail extending laterally is provided on the side of the push movable plate facing away from the sample box module. The first guide rail is slidably engaged with the lifting movable plate. A second guide rail extending vertically is provided on the side of the lifting movable plate facing away from the first guide rail. The second guide rail is slidably engaged with the side plate.

[0020] In some embodiments, the frame further includes a base plate and a vertical plate, with side plates and vertical plates spaced apart on the base plate. The push module further includes a temperature control component, which includes a heat sink and a heating seat. The heat sink is supported on the upper end of the vertical plate, and the heating seat is located on the upper side of the heat sink. A clearance space is formed on the lower side of the sample box module for the heating seat to extend into. The heating seat has a placement hole for the reaction tubes to extend into. When the sample detection box moves to the detection position, the heating seat is used to heat the multiple reaction tubes inside the sample detection box.

[0021] In some embodiments, the heating base includes a heat-conducting plate, a placement cylinder, and a second cover. The heating element and the heat-conducting plate are stacked on the heat dissipation base from bottom to top. The placement cylinder is located on the upper side of the heat-conducting plate, and the second cover covers the outer side of the placement cylinder. A placement hole is opened on the second cover and communicates with the placement cylinder.

[0022] In some embodiments, the heat sink includes a heat sink plate and multiple heat sink fins. The heat sink plate is supported on the upper end of the upright plate, the heating seat is located on the upper side of the heat sink plate, and the multiple heat sink fins are spaced apart on the lower side of the heat sink plate.

[0023] In some embodiments, the ultrasonic magnetic suction module is located below the sample box module and includes:

[0024] An ultrasonic assembly includes a sleeve and an ultrasonic component that is movably inserted into the sleeve along the height direction, with the ultrasonic end of the ultrasonic component extending from the top of the sleeve.

[0025] The drive assembly has a pressure plate mounted on its drive end. The pressure plate is equipped with a magnetic suction element. The drive assembly is used to drive the pressure plate to move between a first position and a second position so that the pressure plate presses down or disengages from the ultrasonic component.

[0026] Specifically, when the pressure plate is in the second position before the detection module detects the sample in the reaction tube, the pressure plate detaches from the ultrasonic component so that the ultrasonic end and the bottom of the reaction tube come into contact, and the magnetic suction component attracts the magnetic beads in the reaction tube from the outer periphery.

[0027] In some embodiments, a drive component is provided at the bottom end of the sleeve, and an elastic element is connected between the drive component and the bottom of the ultrasonic component. In the first position, the drive component and the pressure plate cooperate to adjust the deformation of the elastic element.

[0028] In some embodiments, the top of the sleeve is provided with an end cap, and the end cap has a through hole. The ultrasonic component includes an extension and a piston connected in sequence along the height direction. The piston is movably disposed inside the sleeve and its bottom is connected to an elastic element. The extension extends out from the through hole.

[0029] In some embodiments, the sleeve has an elongated hole that extends along the height of the sleeve. The ultrasonic component includes a guide portion located on the outer periphery of the piston portion. A guide protrusion is formed on the guide portion and extends movably from the elongated hole. The guide protrusion can move along the elongated hole under the action of an external driving force.

[0030] A second aspect of this application provides a sample detection method, applied in the sample detection device described above, the sample detection method comprising the steps of:

[0031] Multiple reaction tubes are loaded into the sample detection kit;

[0032] The control push module drives the sample detection box to move from the loading position to the detection position;

[0033] At the detection location, the extraction module is used to transfer the reagents and samples in the sample detection kit into the reaction tube for transfer and mixing;

[0034] The ultrasonic magnetic attraction module performs magnetic attraction and ultrasonic treatment on the mixed liquid in the reaction tube.

[0035] The detection module amplifies and performs optical detection analysis on the samples after ultrasonic treatment.

[0036] The above technical solution employs an extraction module to lyse and purify the nucleic acid of the target sample, and then uses an ultrasonic magnetic suction module to sonicate the sample in the reaction tube. The detection module performs the same or different nucleic acid amplification detection on each reaction tube, thus enabling the simultaneous detection of multiple targets, improving detection efficiency. The detection module also avoids interference between different reaction tubes during nucleic acid amplification, ensuring more accurate results. Furthermore, the reaction tubes undergo amplification and optical detection within the detection module, and the results are finally processed and analyzed by software, eliminating manual operation throughout the process—sample in, results out—results are generated instantly, resulting in higher efficiency.

[0037] Other features and advantages of the embodiments of this application will be described in detail in the following detailed description section. Attached Figure Description

[0038] The accompanying drawings are provided to further illustrate the embodiments of this application and form part of the specification. They are used together with the following detailed description to explain the embodiments of this application, but do not constitute a limitation on the embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:

[0039] Figure 1 is a schematic diagram of the sample detection device of this application with a portion of the outer shell removed;

[0040] Figure 2 is a schematic diagram of the assembly structure of the sample detection box in the sample detection device of this application;

[0041] Figure 3 is an exploded view of the sample detection box in the sample detection device of this application;

[0042] Figure 4 is an exploded structural diagram of the detection tube and bottom box in the sample detection box of this application;

[0043] Figure 5 is a schematic diagram of the bottom box in the sample detection kit of this application;

[0044] Figure 6 is a schematic diagram of the structure of the housing in the sample detection box of this application;

[0045] Figure 7 is a schematic diagram of the connecting pin structure in the sample detection box of this application;

[0046] Figure 8 is a schematic diagram of the assembly structure of the push module according to an embodiment of the present invention;

[0047] Figure 9 is an exploded structural diagram of a push module according to an embodiment of the present invention;

[0048] Figure 10 is a cross-sectional view of a push module according to an embodiment of the present invention;

[0049] Figure 11 is a structural schematic diagram of the loading seat, the push drive component and the guide component in the push module according to an embodiment of the present invention;

[0050] Figure 12 is a schematic diagram of the frame structure in the sample detection device according to the present invention;

[0051] Figure 13 is a schematic diagram of the temperature control component in the push module according to an embodiment of the present invention;

[0052] Figure 14 is a schematic diagram of the heat sink and heating base in a temperature control assembly according to an embodiment of the present invention;

[0053] Figure 15 is a schematic diagram of the magnetic ultrasonic device of this application during the working process;

[0054] Figure 16 is a schematic diagram of the structure of the magnetic ultrasonic device of this application;

[0055] Figure 17 is a front view schematic diagram of the magnetic ultrasonic device of this application;

[0056] Figure 18 is a cross-sectional view of part BB in Figure 17.

[0057] Explanation of reference numerals in the attached figures

[0058] 10. Reaction tube; 325. Protease tank; 11. Sealing film; 33. First panel; 12. Lyophilized bulb; 331. Placement slot; 20. First cover; 332. Connecting hole; 21. Pipette hole; 333. Connecting sleeve; 22. Positioning groove; 34. Enclosure plate; 23. Pick-up and drop-off hole; 341. Groove; 24. Positioning hole; 35. Limiting sleeve; 25. Positioning protrusion; 36. Reinforcing plate; 30. Base box; 37. Encapsulation film; 31. Detection area; 38. Sealing film; 311. First placement hole; 40. Connecting pin; 32. Liquid storage area; 4 1. Column body; 321. Sample tank; 42. Limiting block; 322. Liquid storage tank; 43. Positioning plate; 323. Pretreatment tank; 44. Deformation tank; 323a. Ultrasonic tank; 45. Pipette tip; 323b. Lysis tank; 324. Collection tank; 50. Loading seat; 821. Second guide rail; 60. Frame; 83. First slider; 61. Guide groove; 84. Second slider; 611. Push guide section; 90. Temperature control component; 612. Lifting guide section; 91. Heat sink; 62. Side plate; 911. Heat sink plate; 63. Base plate; 912. 64. Heat sink fins; 92. Vertical plate; 70. Heating base; 921. Push drive assembly; 71. Heating element; 922. Connecting plate; 923. Second placement hole; 714. Sliding rod; 925. Heat conduction plate; 716. Guide roller; 927. Placement cylinder; 72. Swing rod; 928. Second cover; 729. Slide groove; 93. Heat conduction base; 74. Push drive assembly; 931. Heat conduction channel; 80. Guide assembly; 94. Cooling fan; 81. Push moving plate; 100. Sample detection box; 812. First guide rail; 200. Push module; 8 2. Lifting and moving plate; 300. Detection module; 400. Extraction module; 500. Ultrasonic magnetic suction module; 501. Ultrasonic component; 56. Drive component; 51. Sleeve; 561. Rotary drive component; 511. Long hole; 562. Rotary lead screw; 52. Ultrasonic component; 563. Linear guide rail; 521. Extension; 57. Press plate; 522. Piston part; 571. Clearance opening; 523. Guide protrusion; 572. Position detection component; 53. End cap; 58. Mounting base; 54. Elastic component; 59. Magnetic suction component; 55. Drive component. Detailed Implementation

[0059] The specific embodiments of this application will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit this application.

[0060] The sample testing device according to this application is described below with reference to the accompanying drawings.

[0061] As shown in Figure 1, this application provides a sample detection device, including a frame 60 and a sample box module, a pushing module 200, an extraction module 400, a detection module 300, and an ultrasonic magnetic suction module 500 disposed within the frame 60. The sample box module includes a sample detection box 100 containing multiple reaction tubes 10. The pushing module 200 drives the sample detection box 100 to perform linear movement, so that the sample detection box 100 moves from the loading position to the detection position. The extraction module 400 is mounted on the pushing module 200 and is used to transfer samples and reagents into the reaction tubes 10 for mixing. The detection module 300 is mounted at the end of the pushing module 200 and located at the detection position. The detection module 300 is used to amplify and perform optical detection analysis on the samples in the reaction tubes 10. The ultrasonic magnetic suction module 500 is used to perform ultrasonic treatment on the samples in the reaction tubes 10.

[0062] This application uses an extraction module 400 to lyse and purify the nucleic acid of the target sample to obtain sample nucleic acid, and an ultrasonic magnetic suction module 500 to sonicate the sample in the reaction tube 10. The detection module 300 performs the same or different nucleic acid amplification detection on each reaction tube 10, thereby achieving the simultaneous detection of multiple targets, improving detection efficiency. The detection module 300 also avoids mutual interference between different reaction tubes 10 during nucleic acid amplification detection, ensuring more accurate detection results. Furthermore, the reaction tube 10 undergoes amplification and optical detection in the detection module 300, and the detection results are finally processed and analyzed by software, eliminating manual operation throughout the process—sample in, result out—results are produced immediately, resulting in higher efficiency.

[0063] In some embodiments, the sample box module further includes a loading seat 50 drivenly connected to the push module 200. The sample detection box 100 is placed in the loading seat 50 and includes a first cover 20 and a bottom box 30. The first cover 20 has a pipetting hole 21. The bottom box 30 is rotatably disposed in the first cover 20. The bottom box 30 forms a detection area 31 and a liquid storage area 32. The detection area 31 and the liquid storage area 32 are formed in a circumferential arrangement on the bottom box 30. The liquid storage area 32 is used to store the sample, as well as eluent, water, and mineral water. The reagents, such as petroleum jelly, are used to pretreat the samples. The detection area 31 has multiple first placement holes 311 for placing the reaction tubes 10. The multiple first placement holes 311 are arranged circumferentially along the bottom box 30, and each first placement hole 311 can be used to place one reaction tube 10, so that the multiple reaction tubes 10 can store the same detection reagent or different detection reagents respectively. The liquid storage area 32 and the multiple first placement holes 311 are arranged circumferentially and can be rotated in sequence to communicate with the pipetting hole 21.

[0064] During sample testing, the first cover 20 rotates relative to the bottom box 30 to align the pipetting hole 21 with the storage area 32, thereby enabling pretreatment of the sample in the storage area 32 through the pipetting hole 21. After sample pretreatment, the first cover 20 rotates relative to the bottom box 30 to align the pipetting hole 21 with multiple first placement holes 311, thereby injecting the pretreated sample into multiple reaction tubes 10. The multiple reaction tubes 10 can each contain the same detection reagent or different detection reagents. The sample detection box 100 is then installed into the sample detection device, enabling multiple tests on the same detection indicator at once, greatly improving detection efficiency, or simultaneous detection of multiple detection indicators, achieving simultaneous detection of multiple pathogens.

[0065] In some embodiments, the base box 30 includes a first panel 33 and a surrounding plate 34. The surrounding plate 34 surrounds the periphery of the first panel 33. A placement groove 331 and a liquid storage area 32 are formed on the side of the first panel 33 facing the pipetting hole 21. The side wall of the placement groove 331 forms a detection area 31. A first placement hole 311 is formed on the bottom wall of the placement groove 331. As shown in Figures 3 and 4, each first placement hole 311 is used to place a reaction tube 10, so that the upper end of the reaction tube 10 is flush with or lower than the opening of the placement groove 331. The placement groove 331 serves to accommodate multiple reaction tubes 10 and protect the reaction tubes 10, thereby improving the structural stability and portability of the sample detection box 100.

[0066] Furthermore, the base box 30 also includes multiple limiting sleeves 35, which are located on the side of the first panel 33 facing away from the pipette hole 21, and are respectively connected to multiple first placement holes 311. As shown in Figures 3 to 5, a limiting sleeve 35 is provided on the lower side of the first panel 33 corresponding to the position of each first placement hole 311. The reaction tube 10 passes through the first placement hole 311 and is inserted into the limiting sleeve 35. The limiting sleeve 35 is used to restrict the movement of the reaction tube 10 to stably support the reaction tube 10, effectively preventing the reaction tube 10 from shaking, and further improving the structural stability and portability of the sample detection box 100.

[0067] As shown in Figures 3 to 5, the base box 30 also includes multiple reinforcing plates 36, which are spaced apart on the side of the first panel 33 facing away from the pipette orifice 21. A limiting sleeve 35 is located between the surrounding plate 34 and the multiple reinforcing plates 36. The reinforcing plates 36 strengthen the structural strength of the first panel 33, improving the structural stability of the sample detection box 100, making it suitable for on-site sample detection outdoors.

[0068] As shown in Figures 1 to 6, a connecting hole 332 is provided at the center of the first panel 33. The placement groove 331 and the liquid storage area 32 surround the outer periphery of the connecting hole 332. The sample detection box 100 also includes a connecting pin 40. One end of the connecting pin 40 is rotatably passed through the connecting hole 332 and connected to the first panel 33, and the other end of the connecting pin 40 is connected to the first cover 20 to limit the first cover 20 to the bottom box 30, preventing the first cover 20 from detaching from the bottom box 30. The structure is stable and reliable. Furthermore, the connecting pin 40 can rotate relative to the connecting hole 332, so that the first cover 20 can drive the connecting pin 40 to rotate relative to the bottom box 30. This allows the pipetting hole 21 to communicate sequentially with the liquid storage area 32 and the multiple first placement holes 311, facilitating liquid transfer through the pipetting hole 21 and effectively preventing impurities from being introduced into the liquid storage area 32 and the detection area 31.

[0069] In some embodiments, the first cover 20 has a positioning groove 22 on the side facing away from the bottom box 30. The connecting pin 40 includes a pin body 41, a limiting block 42, and a positioning piece 43. The limiting block 42 and the positioning piece 43 are respectively disposed at both ends of the pin body 41. The pin body 41 passes through the bottom wall of the positioning groove 22 and the connecting hole 332. The limiting block 42 abuts against the side of the panel facing away from the pipetting hole 21. The positioning piece 43 is disposed in the positioning groove 22. As shown in Figures 2 to 7, the positioning groove 22 is used to restrict the rotation of the positioning piece 43 so that the first cover 20 can be connected to the pin body 41 through the positioning piece 43 and drive the pin body 41 to rotate relative to the bottom box 30. The limiting block 42 is connected to the lower end of the pin body 41 and abuts against the lower side of the first panel 33 to limit the first cover 20 to the bottom box 30, preventing the first cover 20 from detaching from the bottom box 30. The structure is stable and reliable.

[0070] In the embodiment of the invention, a connecting sleeve 333 communicating with the connecting hole 332 is provided on the lower side of the first panel 33 at the position corresponding to the connecting hole 332. The column body 41 passes through the connecting hole 332 and the connecting sleeve 333 in sequence, so that the limiting block 42 extends to the lower end of the connecting sleeve 333 and abuts against the lower end of the connecting sleeve 333, so as to limit the first cover 20 on the bottom box 30 and prevent the first cover 20 from detaching from the bottom box 30. The structure is stable and reliable.

[0071] Furthermore, as shown in Figures 5 and 7, the lower end of the column body 41 is provided with multiple spaced deformation grooves 44. When installing the connecting column 40, the limiting block 42 needs to pass through the connecting sleeve 333. The inner wall of the connecting sleeve 333 squeezes the limiting block 42, causing the limiting block 42 to move into the deformation groove 44, which facilitates the contraction of the limiting block 42 and its passage through the connecting sleeve 333. The deformation groove 44 provides deformation space for the movement of the limiting block 42. The structural design is reasonable and improves the ease of assembly.

[0072] In the embodiment of the invention, as shown in Figures 2, 4 and 6, a positioning protrusion 25 is formed on the first cover 20, and a groove 341 is provided on the bottom box 30 at the position corresponding to the positioning protrusion 25 for the positioning protrusion 25 to extend into, so as to position and install the first cover 20 onto the bottom box 30, thereby preventing the first cover 20 from rotating relative to the bottom box 30 before using the sample detection box 100, ensuring the initial performance stability of the sample detection box 100. Furthermore, during sample detection, the first cover 20 rotates relative to the bottom box 30 to tear off the positioning protrusion 25, so as to facilitate pipetting through the pipetting hole 21.

[0073] In the embodiments of the invention, as shown in Figures 3 to 5, an encapsulation film 37 is provided on the upper side of the first panel 33, which is used to seal the liquid storage area 32. A sealing film 38 is provided on the lower side of the first panel 33, which is used to prevent impurities or contaminants from entering the first cover 20 through the connecting sleeve 333 and the connecting hole 332, causing contamination or interference with the detection. Each reaction tube 10 is provided with a sealing film 11, which is used to seal the reaction tube 10 to prevent contamination of the detection reagent. The encapsulation film 37, the sealing film 38 and the sealing film 11 all play a role in improving the overall sealing performance.

[0074] In this embodiment of the invention, a sample tank 321, a liquid storage tank 322, and a pretreatment tank 323 are provided in the liquid storage area 32. The sample tank 321, the liquid storage tank 322, and the pretreatment tank 323 are spaced apart and can be rotated sequentially to communicate with the pipetting orifice 21. As shown in Figures 3 and 4, the sample tank 321 is used to hold the sample, the liquid storage tank 322 is used to hold eluent, water, mineral oil, and other reagents for pretreatment of the sample, and the pretreatment tank 323 can be used to pretreatment the sample. The sample detection box 100 is inserted into the sample detection device so that the ultrasonic magnetic adsorption module 500 can perform ultrasonic magnetic adsorption treatment on the sample pipetted into the pretreatment tank 323.

[0075] Furthermore, a storage groove 324 is provided within the liquid storage area 32, and a pick-and-place hole 23 is provided on the first cover 20 at the position corresponding to the storage groove 324. The sample detection box 100 also includes a pipette tip 45, which passes through the pick-and-place hole 23 and extends into the storage groove 324. As shown in Figures 3 and 4, when the sample detection box 100 is installed in the sample detection device, the extraction module 400 picks up the pipette tip 45 from the pick-and-place hole 23 and drives the pipette tip 45 through the pipetting hole 21 for liquid transfer. The structure has a high degree of integration and prevents impurities from entering the first cover 20 and causing detection contamination, which is conducive to realizing the immediate detection of samples.

[0076] In the embodiment of the invention, the first cover 20 is also provided with a positioning hole 24 spaced apart from the pipetting hole 21. As shown in Figures 3 and 6, the positioning hole 24 is used for the claw of the extraction module 400 to extend into, so that the extraction module 400 rotates the claw to drive the first cover 20 to rotate relative to the bottom box 30, thereby causing the pipetting hole 21 to rotate to communicate with the liquid storage area 32 or any of the first placement holes 311, so that the pipetting tip 45 can extend into the first cover 20 from the pipetting hole 21 to perform pipetting.

[0077] In the embodiment of the invention, as shown in Figures 3 and 4, the storage area 32 contains a sample tank 321, five storage tanks 322, two pretreatment tanks 323, and a protease tank 325. The sample tank 321 is used to hold samples. The five storage tanks 322 are a washing tank, a first elution tank, a second elution tank, a third elution tank, and a mineral oil tank, respectively. The washing tank contains water, the first, second, and third elution tanks contain elution solution, and the mineral oil tank contains mineral oil. The two pretreatment tanks 323... The tanks 323 are an ultrasonic tank 323a and a lysis tank 323b. The lysis tank 323b contains magnetic beads for magnetically attracting samples. The protease tank 325 is used to hold protease. In addition, multiple reaction tubes 10 contain lyophilized bulbs 12 for detecting samples. The lyophilized bulbs 12 are made by freeze-drying the detection reagents. This allows the lyophilized bulbs 12 in multiple reaction tubes 10 to be used to detect the same indicator or to detect different indicators separately, which greatly improves the detection efficiency and realizes the simultaneous detection of multiple pathogens.

[0078] In some embodiments, the frame 60 includes a side plate 62 with a guide groove 61. The guide groove 61 has a horizontally extending push guide section 611 and a vertically extending lifting guide section 612. The push guide section 611 extends in the front-to-back direction, and the lifting guide section 612 extends in the up-down direction. The upper end of the lifting guide section 612 is connected to the rear end of the push guide section 611. The push module 200 includes a connecting plate 71 and a push drive assembly 70. One end of the connecting plate 71 is connected to the loading seat 50, and the other end is provided with a sliding rod 711. The sliding rod 711 is slidably inserted into the guide groove 61. The push drive assembly 70 is used to drive the sliding rod 711 to slide from one end of the push guide section 611 to the lower end of the lifting guide section 612, so that the sliding rod 711 drives the loading seat 50 to move from the loading position to the detection position through the connecting plate 71.

[0079] When the sample test box 100 needs to be placed, the push drive assembly 70 drives the sliding rod 711 to slide to the front end of the push guide section 611, so that the sliding rod 711 drives the loading seat 50 to move to the loading position through the connecting plate 71. When the device is in the position, the loading seat 50 extends from the front end of the side plate 62 to the outside of the frame 60, making it convenient for the operator to place the sample test box 100 on the loading seat 50; after the sample test box 100 is placed, the push drive assembly 70 drives the sliding rod 711 to slide from the front end of the push guide section 611 to the front end of the push guide section 611. At the rear end, the sliding rod 711 moves the loading seat 50 and the sample detection box 100 into the frame 60 via the connecting plate 71. Then, the push drive assembly 70 drives the sliding rod 711 to slide from the upper end of the lifting guide section 612 to the lower end of the lifting guide section 612, so that the sliding rod 711 moves the loading seat 50 down to the detection position via the connecting plate 71. When the loading seat 50 is in the detection position, the sample detection box 100 placed on the loading seat 50 can dock with the detection module 300, thereby enabling the amplification and detection of the sample to be tested in the sample detection box 100.

[0080] In some embodiments, the push drive assembly 70 includes a swing arm 72 and a push drive member 73. The swing arm 72 is swayably disposed on the side plate 62, and a sliding groove 721 is provided on the swing arm 72. The sliding rod 711 can slide through the sliding groove 721 and extend into the guide groove 61. The push drive member 73 is used to drive the swing arm 72 to swing, so that the swing arm 72 drives the sliding rod 711 to slide from one end of the push guide section 611 to the lower end of the lifting guide section 612.

[0081] As shown in Figures 8 to 12, when the sample testing box 100 needs to be placed, the push drive 73 drives the swing rod 72 to swing forward. The swing rod 72 drives the sliding rod 711 in the slide groove 721 to slide to the front end of the push guide section 611, so that the sliding rod 711 drives the loading seat 50 to the loading position through the connecting plate 71. After the sample testing box 100 is placed, the push drive 73 drives the swing rod 72 to swing backward. The swing rod 72 drives the sliding rod 711 in the slide groove 721 to slide from the front end of the push guide section 611 to the lower end of the lifting guide section 612, so that the sliding rod 711 drives the loading seat 50 to the testing position through the connecting plate 71. This realizes the automatic alignment of the sample testing box 100, effectively preventing the sample testing box 100 from shifting, tilting or inaccurately aligning. The alignment is accurate and reliable. In addition, the structure of this application is ingeniously designed, reducing the number of drive components and transmission components, and improving the movement stability and smoothness of the loading seat 50.

[0082] Furthermore, as shown in Figures 8 to 11, a plurality of guide rollers 712 arranged in sequence are sleeved on the sliding rod 711. The guide rollers 712 are used to slide in conjunction with the slide groove 721 or the guide groove 61 to improve the smoothness of the movement of the sliding rod 711 in the slide groove 721 and the guide groove 61. The push drive member 73 can be a motor in the prior art. The output shaft of the motor passes through the side plate 62 and is driven to connect with the swing rod 72. The swing rod 72 is located between the motor and the side plate 62, so that the push drive member 73 can drive the swing rod 72 to swing relative to the side plate 62.

[0083] In some embodiments, the push module 200 further includes a guide component 80, which includes a push moving plate 81 and a lifting moving plate 82. The push moving plate 81 is disposed on the loading seat 50, and a first guide rail 812 extending laterally is provided on the side of the push moving plate 81 facing away from the loading seat 50. The first guide rail 812 is slidably engaged with the lifting moving plate 82. A second guide rail 821 extending vertically is provided on the side of the lifting moving plate 82 facing away from the first guide rail 812. The second guide rail 821 is slidably engaged with the side plate 62.

[0084] As shown in Figures 8 to 11, the connecting plate 71 is connected to the loading seat 50 via the push-moving plate 81, allowing the sliding rod 711 to sequentially move the connecting plate 71, the push-moving plate 81, and the loading seat 50. Furthermore, the loading seat 50 can cause the push-moving plate 81 and the first guide rail 812 to slide relative to the lifting moving plate 82 in the front-back direction. The loading seat 50 can also cause the lifting moving plate 82 and the second guide rail 821 to slide relative to the side plate 62 in the up-down direction via the push-moving plate 81 and the first guide rail 812, improving the smoothness of the loading seat 50's movement.

[0085] Furthermore, as shown in Figures 8 to 11, the guide assembly 80 further includes a first slider 83, which is slidably mounted on the first guide rail 812 and connected to the lifting moving plate 82. The guide assembly 80 also includes a second slider 84, which is slidably mounted on the second guide rail 821 and connected to the side plate 62. The sliding engagement between the second slider 84 and the second guide rail 821 guides the movement of the loading seat 50, improving the alignment accuracy of the sample detection box 100 and the smoothness of the movement of the loading seat 50.

[0086] In some embodiments, the frame 60 further includes a base plate 63 and an upright plate 64, with side plates 62 and upright plates 64 spaced apart on the base plate 63. The push module 200 further includes a temperature control component 90, which includes a heat sink 91 and a heating seat 92. The heat sink 91 is supported on the upper end of the upright plate 64, and the heating seat 92 is located on the upper side of the heat sink 91. The lower side of the loading seat 50 forms a clearance space for the heating seat 92 to extend into. The heating seat 92 is provided with a heating element 921, and a second placement hole 922 for the reaction tube 10 to extend into is provided on the heating seat 92.

[0087] As shown in Figures 9, 12, and 13, two side plates 62 are respectively disposed at the left and right ends of the base plate 63. Two upright plates 64 are provided, spaced apart along the left and right direction on the base plate 63 and located between the two side plates 62. The two ends of the heat sink 91 are respectively connected to the two upright plates 64 so that the two upright plates 64 cooperate to support the heat sink 91. A heating seat 92 is disposed on the heat sink 91, and the detection module 300 is connected to the heating seat 92. When the loading seat 50 moves to the detection position, the heating seat 92 extends into the clearance space under the loading seat 50 to support the loading seat 50. The reaction tube 10 passes through the loading seat 50 and extends into the second placement hole 922. The sample to be tested is contained in the reaction tube 10. The heating element 921 can heat the reaction tube 10. After heating, the heat can be conducted from the heating element 921 to the heat sink 91 for heat dissipation, thus realizing the temperature rise and fall treatment of the sample to be tested in the reaction tube 10. The detection module 300 is connected to the heating element 92. The detection module 300 can directly detect the sample to be tested after the processing. That is, when the push drive component (70) moves the drive loading seat 50 to the detection position, the reaction tube 10 of the sample detection box 100 extends into the second placement hole 922. The temperature control component 90 can perform temperature rise and fall treatment on the reaction tube 10, and the detection module 300 can detect the reaction tube 10.

[0088] In some embodiments, the heating base 92 includes a heat-conducting plate 923, a placement cylinder 924, and a second cover 925. The heating element 921 and the heat-conducting plate 923 are stacked on the heat dissipation base 91 from bottom to top. The placement cylinder 924 is located on the upper side of the heat-conducting plate 923. The second cover 925 covers the outside of the placement cylinder 924. The second placement hole 922 is opened on the second cover 925 and communicates with the placement cylinder 924.

[0089] As shown in Figures 13 and 14, the sample detection box 100 has multiple reaction tubes 10 arranged in sequence. Multiple second placement holes 922 are provided, each corresponding to one of the multiple reaction tubes 10. The optical fiber of the detection module 300 can extend into the placement cylinder 924 to detect the reaction tubes 10. When the loading seat 50 moves to the detection position, the reaction tubes 10 pass through the second placement holes 922 from the lower end of the loading seat 50 and extend into the placement cylinder 924. The heat generated by the heating element 921 is conducted to the placement cylinder 924 through the heat-conducting plate 923 to heat the reaction tubes 10. After heating, the heat is conducted from the heating element 921 to the heat sink 91 for dissipation, thus achieving temperature control of the sample inside the reaction tubes 10. Furthermore, the multiple reaction tubes 10, second placement holes 922, and placement cylinders 924 enable simultaneous detection of multiple reaction tubes 10, offering advantages of high throughput and high efficiency.

[0090] As shown in Figures 13 and 14, the heat sink 91 includes a heat sink 911 and multiple heat sink fins 912. The heat sink 911 is supported on the upper end of the vertical plate 64. The heating seat 92 is located on the upper side of the heat sink 911. The multiple heat sink fins 912 are spaced apart on the lower side of the heat sink 911. The heat generated by the heating element 921 can be dispersed and conducted to the multiple heat sink fins 912 through the heat sink 911, which accelerates the heat dissipation rate, improves the cooling rate, and realizes the rapid heating and cooling cycle of the reaction tube 10.

[0091] As shown in Figures 9 and 13, the temperature control component 90 also includes a heat-conducting base 93 and a cooling fan 94. The heat-conducting base 93 is located between the heat sink 91 and the cooling fan 94, and a heat-conducting channel 931 is formed inside the heat-conducting base 93. The two ends of the heat-conducting channel 931 are respectively set to correspond one-to-one with the heat sink 91 and the cooling fan 94. A heating base 92 is provided on the upper side of the heat sink 91. The heating element 921 in the heating base 92 heats the reaction tube 10, causing the reaction tube 10 to heat up rapidly. When the reaction tube 10 has finished heating, the heating element 921 stops heating. The heat on the reaction tube 10 and the heating element 921 can be quickly transferred to the heat sink 91. The cooling fan 94 is started to make the air in the heat conduction channel 931 flow rapidly, thereby making the heat absorbed on the heat sink 91 quickly transferred to the heat conduction channel 931. The hot air in the heat conduction channel 931 flows along the extension direction of the heat conduction channel 931 and releases heat during the flow. The heat conduction and dissipation speed is fast, which effectively prevents heat from accumulating on the reaction tube 10 and realizes the rapid cooling of the reaction tube 10.

[0092] As shown in Figures 15 and 16, the ultrasonic magnetic attraction module 500 includes an ultrasonic component 501 and a driving component 56. The ultrasonic component 501 includes a sleeve 51 and an ultrasonic component 52 that is movably inserted into the sleeve 51 along the height direction. The ultrasonic end of the ultrasonic component 52 extends from the top of the sleeve 51. A pressure plate 57 is installed on the driving end of the driving component 56. A magnetic attraction element 59 is provided on the pressure plate 57. The driving component 56 is used to drive the pressure plate 57 to move between a first position and a second position. In the first position, the pressure plate 57 presses down on the ultrasonic component 52 to disengage the ultrasonic end from the bottom of the reaction tube 10. In the second position, the pressure plate 57 disengages from the ultrasonic component 52 so that the ultrasonic end abuts against the bottom of the reaction tube 10, and the magnetic attraction element 59 attracts the magnetic beads inside the reaction tube 10 from the outer periphery.

[0093] In this embodiment of the invention, the loading seat 50 is provided with spaced ultrasonic clearance holes and magnetic clearance holes. The ultrasonic clearance holes are used for the ultrasonic end of the ultrasonic component 52 to pass through, so that the ultrasonic end can abut against the reaction tube 10 and perform ultrasonic treatment on the sample in the reaction tube 10. The magnetic clearance holes are used for the end of the magnetic suction component 59 away from the pressure plate 57 to pass through, so that the magnetic suction component 59 can approach the outer peripheral wall of the reaction tube 10 and adsorb the magnetic beads in the reaction tube 10. The loading seat 50 of this application has a high degree of structural integration and improves space utilization.

[0094] Preferably, the first position is located below the second position. When the pressure plate 57 is in the first position, downward pressure is applied to the ultrasonic component 52, causing the ultrasonic end of the ultrasonic component 52 to move downward and disengage from the bottom of the reaction tube 10. When the driving assembly 56 drives the pressure plate 57 upward, the pressure plate 57 stops applying pressure to the ultrasonic component 52, and the ultrasonic end of the ultrasonic component 52 moves upward until it abuts against the bottom of the reaction tube 10, thereby achieving automatic ultrasonic positioning of the reaction tube 10 and improving the ultrasonic effect. Since a receiving cavity is formed inside the reaction tube 10, the receiving cavity contains a sample and magnetic beads are also provided in the receiving cavity. When the ultrasonic end of the ultrasonic component 52 abuts against the bottom of the reaction tube 10, the magnetic suction member 59 applies an adsorption force to the magnetic beads in the receiving cavity, thereby causing the magnetic beads to gather together, and the ultrasonic end of the ultrasonic component 52 performs ultrasound on the sample in the reaction tube 10. This application utilizes the cooperation of the drive assembly 56, the pressure plate 57, and the magnetic suction member 59 to gather the magnetic beads together, thereby preventing any impact on the ultrasonic effect of the ultrasonic component 52 on the sample in the reaction tube 10, and enabling ultrasonication of samples at specific orifices in the reaction tube 10.

[0095] As shown in Figure 15, during ultrasound, the sample in the reaction tube 10 above the ultrasound component 52 can be ultrasounded by moving the ultrasound component 52 in the height direction. When ultrasounding different reaction tubes 10, it is only necessary to control the reaction tube 10 to move above the ultrasound component 52.

[0096] In some embodiments, the magnetic ultrasonic device further includes a mounting base 58, a drive assembly 56 is disposed on the mounting base 58, and a position detection element 572 is provided on the mounting base 58 for detecting the downward displacement of the pressure plate 57.

[0097] The position detection component 572 can be a photoelectric switch. The photoelectric switch is located below the pressure plate 57. When the pressure plate 57 moves between the first position and the second position along the height direction, the pressure plate 57 passes the photoelectric switch. The photoelectric switch senses the displacement of the pressure plate 57 in the height direction and emits a corresponding photoelectric sensing signal, thereby realizing the detection of the downward displacement of the pressure plate 57.

[0098] In some embodiments, the drive assembly 56 includes a rotary drive member 561 and a rotary lead screw 562. The rotary lead screw 562 is threadedly mounted on the drive end of the rotary drive member 561. One end of the pressure plate 57 is connected to the rotary lead screw 562, and the other end is disposed near the position detection member 572. The rotary drive member 561 is a lead screw motor. In the initial position, the lead screw motor drives the rotary lead screw 562 to rotate, thereby driving the pressure plate 57 to move downwards until the photoelectric switch is blocked. Then, the photoelectric switch can emit a photoelectric signal, thereby obtaining the displacement of the pressure plate 57.

[0099] Furthermore, to ensure the smoothness of the pressing plate 57 as it moves up and down along the height direction, the drive assembly 56 also includes a linear guide rail 563 located on the mounting base 58. The linear guide rail 563 extends along the height direction, and the side end face of the pressing plate 57 slides in cooperation with the linear guide rail 563. When the rotating screw 562 drives the pressing plate 57 to move up and down, the pressing plate 57 simultaneously slides along the linear guide rail 563, thereby ensuring the smooth and precise movement of the pressing plate 57 and preventing horizontal swaying.

[0100] In some embodiments, a clearance opening 571 is formed on the pressure plate 57, through which the sleeve 51 passes. The clearance opening 571 is an arc-shaped concave notch, allowing the sleeve 51 to pass through, and a certain gap exists between the inner peripheral wall of the clearance opening 571 and the outer peripheral wall of the sleeve 51. Since the magnetic suction member 59 is fixed to the edge of the pressure plate 57 near the clearance opening 571, a very small gap exists between the magnetic suction member 59 and the outer peripheral wall of the reaction tube 10. When the ultrasonic component 52 abuts against the bottom of the reaction tube 10, the strong magnetic force can gather the magnetic beads inside the reaction tube 10 together, preventing any impact on the ultrasonic effect on the sample inside the reaction tube 10.

[0101] In some embodiments, the magnetic attractor 59 is a vertically extending block structure, and the side of the magnetic attractor 59 facing the sleeve 51 is the magnetic attracting surface. Preferably, the magnetic attractor 59 is a magnet, and the magnetic attracting surface of the magnet is disposed facing the outer peripheral wall of the sleeve 51. In this way, when the ultrasonic end of the ultrasonic component 52 abuts against the reaction tube 10, the magnet applies a strong magnetic attraction force to the magnetic beads in the reaction tube 10, causing the magnetic beads to gather on the side close to the magnet.

[0102] In some embodiments, a driving component 55 is provided at the bottom end of the sleeve 51, and an elastic element 54 is connected between the driving component 55 and the bottom of the ultrasonic component 52. In the first position, the driving component 55 and the pressure plate 57 cooperate to adjust the deformation of the elastic element 54. The elastic element 54 is a spring in the prior art, and the ultrasonic component 52 is an ultrasonic transducer in the prior art.

[0103] In this embodiment, a storage cavity is formed inside the sleeve 51. The top end of the ultrasonic component 52 is used to contact the external reaction tube 10 and to ultrasonically break up the sample stored inside the reaction tube 10. The ultrasonic component 52 is disposed in the storage cavity and can move relative to the sleeve 51 under the action of the elastic member 54. When the ultrasonic component 52 moves downward relative to the sleeve 51, it can separate from the reaction tube 10. At this time, the ultrasonic component 52 can be horizontally adjusted so that the top of the ultrasonic component 52 can be efficiently positioned and engaged with the bottom of the reaction tube 10. Then, the ultrasonic component 52 is driven to move upward relative to the sleeve 51, and the ultrasonic component 52 can re-abut against the reaction tube 10, ensuring that the ultrasonic component 52 has a good ultrasonic breaking effect. A driving component 55 is provided at the bottom end of the sleeve 51. An elastic element 54 is elastically compressed between the driving component 55 and the bottom end of the ultrasonic component 52. By moving the driving component 55 and the pressure plate 57 up and down along the height direction, the deformation of the elastic element 54 can be adjusted so that the ultrasonic component 52 is pressed by the elastic element 54 to achieve the optimal ultrasonic fragmentation state and improve the ultrasonic fragmentation effect. In this embodiment, the ultrasonic effect transmitted by the ultrasonic component 52 to the reaction tube 10 is different under different pressures. By driving the elastic element 54 with the driving component 55, the deformation of the elastic element 54 can be adjusted so that the elastic element 54 can transmit pressure to the ultrasonic component 52. The ultrasonic component 52 bears different pressures under different deformations of the elastic element 54. By adjusting multiple times, the ultrasonic component 52 can be driven to achieve the optimal ultrasonic fragmentation state.

[0104] As shown in Figures 17 and 18, the top end of the sleeve 51 is provided with an end cap 53, and the end cap 53 has a through hole. The ultrasonic component 52 includes an extension 521 and a piston 522 connected sequentially along the height direction. The piston 522 is movably disposed inside the sleeve 51 and its bottom is connected to the elastic member 54. The extension 521 extends out from the through hole.

[0105] The end cap 53 has a through hole in the middle. Preferably, the size of the extension 521 matches the size of the through hole, allowing the extension 521 to extend out of the through hole. This avoids gaps between the extension 521 and the through hole due to poor fit, which could allow external debris to easily enter the storage cavity and affect the normal operation of the ultrasonic component 52. The bottom of the piston 522 is elastically connected to the elastic member 54, allowing the piston 522 to respond more sensitively to the force generated during the elastic deformation of the elastic member 54. Thus, when the piston 522 moves downward to compress the elastic member 54 via the guide protrusion 523, the elastic member 54 will exert an upward force on the piston 522 after the force applied to the guide protrusion 523 disappears. This causes the piston 522 to move upward and position the extension 521 in conjunction with the bottom of the reaction tube 10.

[0106] Furthermore, the top surface of the extension 521 has a receiving groove adapted to the bottom of the reaction tube 10. The top of the extension 521 can be designed to conform to the shape of the reaction tube 10, which facilitates the transfer of more energy to the reaction tube 10. With the receiving groove, the bottom of the reaction tube 10 can fully contact the top of the extension 521, improving the ultrasound transmission effect and also limiting the position of the reaction tube 10, preventing it from detaching from the extension 521 during ultrasound operation due to poor fit, thus affecting the normal operation of the sample cell ultrasound disruption.

[0107] In some embodiments, the sleeve 51 has an elongated hole 511 extending along the height of the sleeve 51. The ultrasonic component 52 includes a guide portion located on the outer periphery of the piston portion 522. A guide protrusion 523 is formed on the guide portion and extends movably from the elongated hole 511. The guide protrusion 523 can move along the elongated hole 511 under the action of an external driving force, thereby driving the piston portion 522 connected to the guide portion to move together. The guide protrusion 523 is located below the pressure plate 57. When the pressure plate 57 moves downward, it presses down the guide protrusion 523, causing the piston portion 522 to press against the elastic member 54. After adjusting the position relative to the reaction tube 10, it is released. Under the elastic recovery of the elastic member 54, the guide protrusion 523 moves upward along the elongated hole 511, and the piston portion 522 drives the extension portion 521 to move upward together, so that the extension portion 521 docks with the bottom of the reaction tube 10.

[0108] Furthermore, two elongated holes 511 are formed on the sleeve 51, and the two elongated holes 511 are symmetrically arranged on the side wall of the sleeve 51. Similarly, a guide protrusion 523 is symmetrically provided on each side of the guide portion. During the process of aligning the extension portion 521 with the bottom of the reaction tube 10, the pressure plate 57 first moves downward and simultaneously presses down the guide protrusions 523 on both sides, causing the guide protrusions 523 to move along the elongated holes 511, thereby driving the piston portion 522 to move downward, thereby compressing the elastic member 54 and putting the elastic member 54 in a compressed state. At this time, the extension portion 521 is not in contact with the bottom of the reaction tube 10, so the position of the sleeve 51 can be adjusted horizontally to allow the extension portion 521 to better cooperate with the bottom of the reaction tube 10. After adjustment, the control press plate 57 moves upward and disengages from the guide protrusion 523. Under the elastic recovery of the elastic element 54, the guide protrusion 523 moves upward along the elongated hole 511, and the piston part 522 drives the extension part 521 to move upward together, so that the extension part 521 docks with the reaction tube 10.

[0109] In other embodiments, the outer periphery of the drive component 55 is threadedly rotated with the inner peripheral wall of the sleeve 51. By rotating the drive component 55, the compression amount of the elastic element 54 can be adjusted, and the ultrasonic breaking effect of the ultrasonic component 52 under different compression amounts can be tested experimentally, thereby adjusting the ultrasonic component 52 to the optimal ultrasonic breaking state.

[0110] In this embodiment, the sleeve 51 is made of plastic, which is lightweight and has high structural strength while achieving good weight reduction. It can effectively transmit the pressure of the elastic element 54 without being easily crushed.

[0111] A second aspect of this application provides a sample detection method, applied in the sample detection device described above, the sample detection method comprising the steps of:

[0112] Multiple reaction tubes 10 are loaded into the sample detection box 100;

[0113] The control push module 200 drives the sample detection box 100 to move from the loading position to the detection position;

[0114] At the detection location, the extraction module 400 is used to transfer the reagents and samples in the sample detection box 100 into the reaction tube 10 and mix them.

[0115] The ultrasonic magnetic attraction module 500 performs magnetic attraction ultrasonic treatment on the mixed liquid in the reaction tube 10.

[0116] The detection module 300 amplifies and performs optical detection analysis on the samples after ultrasonic treatment.

[0117] To further facilitate understanding of the working principle of the sample testing device of this application, the entire sample testing process of the sample testing device is described in detail below:

[0118] During sample testing, the operator opens the sealing cap 46 and injects the sample into the sample tank 321. After sample addition, the sample testing box 100 is installed into the sample testing equipment. The heating device of the sample testing equipment preheats the lysis tank 323b. The extraction module 400 descends so that the claws of the extraction module 400 extend into the positioning hole 24 and the pipette pump of the extraction module 400 picks up the pipette tip. The extraction module 400 drives the first cover 20 to rotate through the claws so that the pipette hole 21 is aligned with the sample. The sample tank 321 is open to the ultrasonic tank 323a. A pipette tip 45 draws a sample from the sample tank 321 through the pipette orifice 21 and injects the sample into the ultrasonic tank 323a. The ultrasonic magnetic suction module 500 of the sample detection equipment performs ultrasonic lysis on the sample in the reaction tube 10. After ultrasonic lysis, the pipette tip 45 transfers the sample into the lysis tank 323b. Simultaneously, the pipette tip 45 draws protease from the protease tank 325 and transfers it into the lysis tank 323b. A heating device heats the sample in the lysis tank 323b. The lysis process involves the ultrasonic magnetic adsorption module 500 using a magnet to adsorb magnetic beads. After adsorption, the pipette tip 45 transfers the waste liquid to the sample tank 321. The pipette tip 45 also draws eluent from the first elution tank and transfers it to the lysis tank 323b, and from the second elution tank and transfers it to the lysis tank 323b. The pretreatment device removes the magnet, and the extraction module 400 drives the pipette tip 45 to repeatedly agitate and mix the sample in the lysis tank 323b. Then, the pretreatment device again uses a magnet... After magnetic bead adsorption, the pipette tip 45 transfers the waste liquid to the first elution tank and the second elution tank respectively. After the waste liquid is transferred, the pipette tip 45 is inserted into the cleaning tank for cleaning. After cleaning, the pipette tip 45 draws the eluent from the third elution tank and transfers it to the lysis tank 323b. The sample is then eluted again by magnetic adsorption and mixing with the magnetic beads using a magnet to complete the sample pretreatment. Then, the detection module 300 amplifies and performs optical detection analysis on the ultrasonically treated sample.

[0119] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0120] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0121] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. A sample testing device, characterized in that, The system includes a frame (60) and the following components disposed within the frame (60): a sample box module, including a sample detection box (100) containing multiple reaction tubes (10); a push module (200) for driving the sample detection box (100) to perform linear motion so that the sample detection box (100) moves from a loading position to a detection position; an extraction module (400) mounted on the push module (200) for transferring samples and reagents into the reaction tubes (10) for mixing; a detection module (300) mounted at the end of the push module (200) and located at the detection position, the detection module (300) for amplifying and optically detecting and analyzing the samples in the reaction tubes (10); and an ultrasonic magnetic suction module (500) for ultrasonically treating the liquid in the reaction tubes (10).

2. The sample detection device according to claim 1, characterized in that, The sample box module also includes a loading seat (50) that is driven and connected to the push module (200). The push module (200) is used to drive the loading seat (50) to move linearly from the loading position to the detection position. The sample detection box (100) is placed in the loading seat (50) and includes: a first cover (20) with a pipette hole (21); a bottom box (30) which is rotatably disposed in the first cover (20). The bottom box (30) forms a detection area (31) and a liquid storage area (32). The liquid storage area (32) is used to store the sample and reagents for pretreatment of the sample. The detection area (31) has a plurality of first placement holes (311) for placing the reaction tube (10). The liquid storage area (32) and the plurality of first placement holes (311) are arranged in a circumferential interval and can be rotated sequentially to communicate with the pipette hole (21).

3. The sample detection device according to claim 2, characterized in that, The bottom box (30) includes a panel and a surrounding plate (34). The surrounding plate (34) surrounds the periphery of the panel. A placement groove (331) and the liquid storage area (32) are formed on the side of the panel facing the pipetting hole (21). The side wall of the placement groove (331) forms the detection area (31). The first placement hole (311) is opened on the bottom wall of the placement groove (331).

4. The sample detection device according to claim 3, characterized in that, The panel has a connection hole (332), the placement groove (331) and the liquid storage area (32) are arranged around the outer periphery of the connection hole (332), the sample detection box (100) also includes a connecting pin (40), one end of the connecting pin (40) is rotatably passed through the connection hole (332) and connected to the panel, and the other end of the connecting pin (40) is connected to the first cover (20).

5. The sample detection device according to claim 1, characterized in that, The frame (60) includes a side plate (62) with a guide groove (61). The guide groove (61) has a horizontally extending push guide section (611) and a vertically extending lifting guide section (612). The push module (200) includes: a connecting plate (71), one end of which is connected to the sample box module, and the other end of which is provided with a sliding rod (711). The sliding rod (711) is slidably inserted into the guide groove (61); and a push drive assembly (70) for driving the sliding rod (711) to slide from one end of the push guide section (611) to the lower end of the lifting guide section (612), so that the sliding rod (711) drives the sample box module to move from the loading position to the detection position through the connecting plate (71).

6. The sample detection device according to claim 5, characterized in that, The push drive assembly (70) includes a swing arm (72) and a push drive component (73). The swing arm (72) is swayably mounted on the side plate (62), and a sliding groove (721) is provided on the swing arm (72). The sliding rod (711) can slide through the sliding groove (721) and extend into the guide groove (61). The push drive component (73) is used to drive the swing arm (72) to swing, so that the swing arm (72) drives the sliding rod (711) to slide from one end of the push guide section (611) to the lower end of the lifting guide section (612).

7. The sample detection device according to claim 5, characterized in that, The push module (200) further includes a guide component (80), which includes a push moving plate (81) and a lifting moving plate (82). The push moving plate (81) is disposed on the sample box module, and the push moving plate (81) has a first guide rail (812) extending laterally on the side facing away from the sample box module. The first guide rail (812) is slidably engaged with the lifting moving plate (82). The lifting moving plate (82) has a second guide rail (821) extending vertically on the side facing away from the first guide rail (812). The second guide rail (821) is slidably engaged with the side plate (62).

8. The sample detection device according to claim 5, characterized in that, The frame (60) also includes a base plate (63) and a vertical plate (64). The side plate (62) and the vertical plate (64) are spaced apart on the base plate (63). The push module (200) also includes a temperature control component (90). The temperature control component (90) includes a heat sink (91) and a heating seat (92). The heat sink (91) is supported on the upper end of the vertical plate (64). The heating seat (92) is located on the upper side of the heat sink (91). The lower side of the sample box module has a clearance space for the heating seat (92) to extend into. The heating seat (92) has a second placement hole (922) for the reaction tube (10) to extend into. When the sample detection box (100) moves to the detection position, the heating seat (92) is used to heat the multiple reaction tubes in the sample detection box (100).

9. The sample detection device according to claim 8, characterized in that, The heating base (92) includes a heat-conducting plate (923), a placement cylinder (924), and a second cover (925). The heating element (921) and the heat-conducting plate (923) are stacked on the heat dissipation base (91) from bottom to top. The placement cylinder (924) is located on the upper side of the heat-conducting plate (923). The second cover (925) covers the outside of the placement cylinder (924). The second placement hole (922) is opened on the second cover (925) and communicates with the placement cylinder (924).

10. The sample detection device according to claim 1, characterized in that, The ultrasonic magnetic suction module (500) is located below the sample box module and includes: an ultrasonic component (501), including a sleeve (51) and an ultrasonic component (52) movably inserted into the sleeve (51) along the height direction, the ultrasonic end of the ultrasonic component (52) extending from the top of the sleeve (51); a driving component (56), the driving end of the driving component (56) being equipped with a pressure plate (57), the pressure plate (57) being provided with a magnetic suction element (59), the driving component (56) being used to drive the sample box module. The pressure plate (57) moves between a first position and a second position to press down or detach from the ultrasonic component (52); wherein, when the pressure plate (57) is in the second position before the detection module (300) detects the sample in the reaction tube (10), the pressure plate (57) detaches from the ultrasonic component (52) so that the ultrasonic end abuts against the bottom of the reaction tube (10), and the magnetic attractor (59) attracts the magnetic beads in the reaction tube (10) from the periphery.

11. The sample detection device according to claim 10, characterized in that, The bottom end of the sleeve (51) is provided with a driving component (55), and an elastic element (54) is connected between the bottom of the driving component (55) and the ultrasonic component (52). In the first position, the driving component (55) and the pressure plate (57) cooperate to adjust the deformation of the elastic element (54).

12. The sample detection device according to claim 11, characterized in that, The top end of the sleeve (51) is provided with an end cap (53), and the end cap (53) has a through hole. The ultrasonic component (52) includes an extension (521) and a piston (522) connected sequentially along the height direction. The piston (522) is movably disposed inside the sleeve (51) and its bottom is connected to the elastic member (54). The extension (521) extends out from the through hole.

13. The sample detection device according to claim 12, characterized in that, The sleeve (51) has an elongated hole (511) extending along the height direction of the sleeve (51). The ultrasonic component (52) includes a guide portion located on the outer periphery of the piston portion (522). A guide protrusion (523) is formed on the guide portion. The guide protrusion (523) extends movably from the elongated hole (511) and can move along the elongated hole (511) under the action of an external driving force.

14. A sample detection method, characterized in that, The sample detection method, applied to any one of claims 1 to 13, comprises the following steps: loading a plurality of reaction tubes (10) into a sample detection box (100); controlling a push module (200) to drive the sample detection box (100) from the loading position to the detection position; at the detection position, an extraction module (400) transfers the reagents and samples in the sample detection box (100) into the reaction tubes (10) and mixes them; an ultrasonic magnetic suction module (500) performs magnetic suction ultrasonic treatment on the mixed liquid in the reaction tubes (10); and a detection module (300) amplifies and performs optical detection analysis on the ultrasonically treated sample.

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