Culture medium for rapidly amplifying organoid

Abstract

A culture medium for rapid amplification of organoids comprises connecting rings, a guide sleeve movably connected between the two connecting rings, a first movable sleeve sleeved on the connecting ring at the head, a second movable sleeve movably sleeved on the connecting ring at the tail, a support fixedly connected to the lower end of the guide sleeve, a support fixedly connected to the lower end of the second movable sleeve, and a connecting rod fixedly connected to the support. And test tubes are inserted into the guide sleeve, the first movable sleeve and the second movable sleeve, and are filled with culture media. A stand posture is kept through the supporting column and the support, a test tube is inserted into the guide sleeve, the first movable sleeve and the second movable sleeve, different culture media are placed in the test tube, after completion, the connecting ring, the guide sleeve and the first movable sleeve are rolled up, the test tube is folded by rolling up the connecting ring, the guide sleeve and the first movable sleeve, and the space occupation range is reduced; the test tube is convenient to operate and observe by utilizing the unfolded state, and the overall practicability is improved.

Description

A culture medium for rapid expansion of organoids Technical Field

[0001] This invention belongs to the field of medical device technology, and in particular relates to a culture medium for the rapid expansion of organoids. Background Technology

[0002] The culture medium for rapid expansion of organoids is usually based on Advanced DMEM / F12 (containing GlutaMAX and HEPES), using a serum-free / conditionless medium formulation, and supplemented with core proliferation factors (RSPO1, Wnt3a, EGF, Noggin), anti-differentiation small molecules (such as A83-01), anti-apoptotic / anti-stress agents (Y-27632, N-acetylcysteine, nicotinamide) and supplements such as B27 / N2. It is combined with matrix gels such as Matrigel to achieve 3D suspension expansion. The purpose is to maintain the Lgr5+ stem cell pool, inhibit differentiation, accelerate proliferation and improve passage survival rate. It is commonly used for the rapid propagation and recovery of organoids such as intestine, stomach and mammary gland after cryopreservation and thawing.

[0003] Various culture media can usually be cultured using a set of test tubes. Conventional test tube racks can only be arranged in a single line. When refrigerating, the test tubes need to be removed and the rack placed back in, which wastes space. However, when using space-saving racks to constrain the test tubes, the test tubes are piled up together, obstructing the view and making it impossible to observe normally. This causes the problem that conventional culture media sets used for rapid expansion of organoids are inconvenient to pack up and unpack.

[0004] To address these issues, we propose a culture medium for the rapid expansion of organoids. Summary of the Invention

[0005] The purpose of this invention is to solve the problem that conventional culture media used for rapid expansion of organoids are inconvenient to collect and distribute, and to propose a culture medium for rapid expansion of organoids.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a culture medium for rapid amplification of organoids, comprising connecting rings, a guide sleeve movably connected between two connecting rings, a first movable sleeve fitted on the head connecting ring, and a second movable sleeve movably fitted on the tail connecting ring, a support fixedly connected to the lower end of the guide sleeve, and a bracket fixedly connected to the lower end of the second movable sleeve, with test tubes containing culture medium inserted inside the guide sleeve, the first movable sleeve, and the second movable sleeve. The connecting rings, guide sleeves, the first movable sleeve, and the second movable sleeve are unfolded and straightened, maintaining an upright posture using the support and bracket. Test tubes are then inserted into the guide sleeves, the first movable sleeve, and the second movable sleeve, and different culture media are placed into the test tubes. After completion, the connecting rings, guide sleeves, and the first movable sleeve are rolled up. By rolling up the connecting rings, guide sleeves, and the first movable sleeve, the test tubes are stored away, reducing space occupation. The unfolded state facilitates operation and observation of the test tubes, improving overall practicality.

[0007] Preferably, the guide sleeve includes a first sleeve body and a second sleeve body, with a first connecting block fixedly connected between the first and second sleeve bodies. A first washer ring is fixedly connected to the lower part of the first sleeve body, and a second washer ring is fixedly connected to the upper part of the second sleeve body. The first and second sleeve bodies are movably fitted onto two connecting rings, with the first and second washer rings in movable contact with the connecting rings. The first and second sleeve bodies movably constrain two adjacent connecting rings, keeping them linked and facilitating unfolding or rewinding operations.

[0008] Preferably, the first movable sleeve includes a third sleeve body, which is fitted onto the connecting ring. A third washer ring is fixedly connected to the upper part of the third sleeve body, and the third washer ring is movably embedded within the connecting ring. The third washer ring, positioned within the connecting ring at the head, ensures the stability of the constrained test tube.

[0009] Preferably, the second movable sleeve includes a fourth sleeve body, which is movably fitted onto a connecting ring located at the end. A fourth washer ring is fixedly connected to the lower part of the fourth sleeve body, and a second connecting block is fixedly connected to the outside of the fourth sleeve body. The fourth washer ring is used to place between the test tube and the connecting ring to ensure the stability of the test tube placement, and the second connecting block is used to connect the support.

[0010] Preferably, the support column includes a first column, the lower end of which is fixedly connected to a support block, and the upper end of which is fixedly connected to a first connecting block. The support block increases the contact area between the support column and the table surface.

[0011] Preferably, the support includes a second column, the upper end of which is fixedly connected to a second connecting block, and the lower end of which is fixedly connected to an arc-shaped support leg. The arc-shaped support leg increases the support range, allowing the device to remain upright after unfolding. When the device is rolled up, the arc-shaped support leg conforms to the arc-shaped posture, facilitating a reduction in the area occupied during the winding process.

[0012] In summary, the technical effects and advantages of this invention are as follows: 1. By unfolding and straightening the connecting ring, guide sleeve, first movable sleeve, and second movable sleeve, and using the support pillar and bracket to maintain the upright posture, the test tube is inserted into the guide sleeve, first movable sleeve, and second movable sleeve, and different culture media are placed into the test tube. After completion, the connecting ring, guide sleeve, and first movable sleeve are rolled up. By rolling up the connecting ring, guide sleeve, and first movable sleeve, the test tube is stored away, reducing the space occupied. The unfolded state facilitates the operation and observation of the test tube, thus improving the overall practicality.

[0013] 2. By using the first and second sets of bodies to dynamically constrain two adjacent connecting rings, the connecting rings remain linked, facilitating unfolding or rewinding operations.

[0014] 3. The curved legs increase the support range, allowing the device to stand upright after being unfolded. When the device is rolled up, the curved legs match the curved shape, making it easier to reduce the area occupied during the winding process. Attached Figure Description

[0015] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is a schematic diagram of the guide sleeve structure of the present invention; Figure 3 is a schematic diagram of the first movable sleeve structure of the present invention; Figure 4 is a schematic diagram of the second movable sleeve structure of the present invention; Figure 5 is a schematic diagram of the support structure of the present invention; Figure 6 is a schematic diagram of the bracket structure of the present invention.

[0016] In the diagram: 1. Connecting ring; 2. Guide sleeve; 3. First movable sleeve; 4. Second movable sleeve; 5. Support column; 6. Bracket; 7. Test tube; 21. First sleeve body; 22. First connecting block; 23. Second sleeve body; 24. Second washer ring; 25. First washer ring; 31. Third sleeve body; 32. Third washer ring; 41. Fourth washer ring; 42. Fourth sleeve body; 43. Second connecting block; 51. First column; 52. Support block; 61. Second column; 62. Arc-shaped support foot. Detailed Implementation

[0017] The technical solutions in the embodiments of the invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the invention, and not all embodiments.

[0018] As shown in Figure 1, a culture medium for rapid organoid amplification includes connecting rings 1, with guide sleeves 2 movably connected between two connecting rings 1. A first movable sleeve 3 is fitted onto the head connecting ring 1, and a second movable sleeve 4 is movably fitted onto the tail connecting ring 1. A support post 5 is fixedly connected to the lower end of the guide sleeve 2, and a support 6 is fixedly connected to the lower end of the second movable sleeve 4. Test tubes 7 containing culture medium are inserted into the guide sleeves 2, the first movable sleeve 3, and the second movable sleeve 4. The connecting rings 1, guide sleeves 2, the first movable sleeve 3, and the second movable sleeve 4 are unfolded and straightened, and the support post 5 and the support 6 are used to maintain an upright posture. The test tubes 7 are then inserted into the guide sleeves 2, the first movable sleeve 3, and the second movable sleeve 4, and different culture media are placed into the test tubes 7. After completion, the connecting rings 1, guide sleeves 2, and the first movable sleeve 3 are rolled up.

[0019] As shown in Figures 1 and 2, the guide sleeve 2 includes a first sleeve body 21 and a second sleeve body 23. A first connecting block 22 is fixedly connected between the first sleeve body 21 and the second sleeve body 23. A first washer ring 25 is fixedly connected to the lower part of the first sleeve body 21, and a second washer ring 24 is fixedly connected to the upper part of the second sleeve body 23. The first sleeve body 21 and the second sleeve body 23 are respectively movably fitted onto two connecting rings 1. The first washer ring 25 and the second washer ring 24 are in movable contact with the connecting rings 1. The first sleeve body 21 and the second sleeve body 23 are used to movably constrain two adjacent connecting rings 1, keeping the connecting rings 1 connected.

[0020] As shown in Figures 1 and 3, the first movable sleeve 3 includes a third sleeve body 31, which is fitted onto the connecting ring 1. A third washer ring 32 is fixedly connected to the upper part of the third sleeve body 31, and the third washer ring 32 is movably embedded in the connecting ring 1. The stability of the constrained test tube 7 is ensured by the third washer ring 32 padding the connecting ring 1 located at the head.

[0021] As shown in Figures 1 and 4, the second movable sleeve 4 includes a fourth sleeve body 42, which is movably fitted onto the connecting ring 1 located at the end. A fourth pad ring 41 is fixedly connected to the lower part of the fourth sleeve body 42, and a second connecting block 43 is fixedly connected to the outside of the fourth sleeve body 42. The fourth pad ring 41 is used to place between the test tube 7 and the connecting ring 1 to ensure the stability of the test tube 7. The second connecting block 43 is used to connect the support 6.

[0022] As shown in Figures 1, 2, and 5, the support column 5 includes a first column 51, with a support block 52 fixedly connected to the lower end of the first column 51, and the upper end of the first column 51 fixedly connected to a first connecting block 22. The support block 52 is used to increase the contact area between the support column 5 and the table surface.

[0023] As shown in Figures 1, 4, and 6, the support 6 includes a second column 61, the upper end of which is fixedly connected to the second connecting block 43, and the lower end of which is fixedly connected to an arc-shaped support leg 62. The arc-shaped support leg 62 increases the support range, allowing the device to stand upright after being unfolded, and when the device is rolled up, the arc shape of the arc-shaped support leg 62 matches the arc-shaped posture.

[0024] Working principle: Unfold and straighten the connecting ring 1, guide sleeve 2, first movable sleeve 3, and second movable sleeve 4. Use the support column 5 and bracket 6 to maintain the upright posture. Insert the test tube 7 into the guide sleeve 2, first movable sleeve 3, and second movable sleeve 4. Put different culture media into the test tube 7. After completion, roll up the connecting ring 1, guide sleeve 2, and first movable sleeve 3.

[0025] The above description is merely a preferred embodiment of the invention, but the scope of protection of the invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the invention, based on the technical solution and inventive concept of the invention, should be included within the scope of protection of the invention.

[0026] The description briefly mentions the application directions of the invention to existing technologies known to those skilled in the art without modification, which are combined with the invention to form a complete technology; it avoids excessive popularization of technologies familiar to those skilled in the art, in order to help those skilled in the art quickly understand the main content of the invention.

Claims

1. A culture medium for rapid expansion of organoids, characterized in that: It includes a connecting ring (1), a guide sleeve (2) is movably connected between the two connecting rings (1), a first movable sleeve (3) is fitted on the connecting ring (1) at the head, and a second movable sleeve (4) is movably fitted on the connecting ring (1) at the end. A support column (5) is fixedly connected to the lower end of the guide sleeve (2), and a bracket (6) is fixedly connected to the lower end of the second movable sleeve (4). Test tubes (7) are inserted into the guide sleeve (2), the first movable sleeve (3), and the second movable sleeve (4), and the test tubes (7) are filled with culture medium.

2. The culture medium for rapid expansion of organoids according to claim 1, characterized in that: The guide sleeve (2) includes a first sleeve body (21) and a second sleeve body (23). A first connecting block (22) is fixedly connected between the first sleeve body (21) and the second sleeve body (23). A first washer ring (25) is fixedly connected to the lower part of the first sleeve body (21), and a second washer ring (24) is fixedly connected to the upper part of the second sleeve body (23). The first sleeve body (21) and the second sleeve body (23) are respectively movably sleeved on the two connecting rings (1). The first washer ring (25) is in movable contact with the connecting ring (1), and the second washer ring (24) is in movable contact with the connecting ring (1).

3. The culture medium for rapid expansion of organoids according to claim 1, characterized in that: The first movable sleeve (3) includes a third sleeve body (31), which is sleeved on the connecting ring (1). A third pad ring (32) is fixedly connected to the upper part of the third sleeve body (31), and the third pad ring (32) is movably embedded in the connecting ring (1).

4. The culture medium for rapid expansion of organoids according to claim 1, characterized in that: The second movable sleeve (4) includes a fourth sleeve body (42), which is movably sleeved on the connecting ring (1) located at the end. A fourth pad ring (41) is fixedly connected to the lower part of the fourth sleeve body (42), and a second connecting block (43) is fixedly connected to the outside of the fourth sleeve body (42).

5. A culture medium for rapid expansion of organoids according to claim 2, characterized in that: The support column (5) includes a first column (51), the lower end of which is fixedly connected to a support block (52), and the upper end of which is fixedly connected to a first connecting block (22).

6. The culture medium for rapid expansion of organoids according to claim 4, characterized in that: The bracket (6) includes a second column (61), the upper end of which is fixedly connected to a second connecting block (43), and the lower end of which is fixedly connected to an arc-shaped support leg (62).

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