Sample processing system

Abstract

The invention relates to the technical field of biological sample treatment, in particular to a sample treatment system which comprises a micro-fluidic chip, a positive pressure source and a negative pressure source, the micro-fluidic chip comprises a reaction chamber, a first chamber and a liquid outlet side, the first chamber is communicated with the reaction chamber and is used for storing reagents; a filtering structure is arranged between the reaction chamber and the liquid outlet side, and waste liquid output by the reaction chamber is filtered by the filtering structure and then is discharged to the liquid outlet side; the positive pressure source is used for providing positive pressure to drive the reagent in the first chamber to be pressed into the reaction chamber; the negative pressure source is used for providing negative pressure to drive waste liquid output by the reaction chamber to flow to the liquid outlet side after being filtered by the filtering structure; according to the invention, a sample reaction place is transferred from conventional experimental consumables to the micro-fluidic chip for operation, so that the steps of reagent adding, reaction, waste liquid removal and the like are all carried out on the chip, the use of a mechanical arm for pipetting is avoided, the integration of the system is improved, and the volume of the instrument is reduced; and the design of the filtering structure can reduce sample loss during waste liquid output.

Description

Technical Field

[0001] This invention relates to the field of biological sample processing technology, and more specifically to a sample processing system. Background Technology

[0002] In the field of life science research, biological samples often require pretreatment. The routine steps of sample pretreatment usually include adding reagents, reacting under certain conditions (appropriate reaction temperature, etc.), washing, and removing waste liquid. These repetitive and tedious steps often consume a lot of time and energy of the experimenters.

[0003] In response, commercially available automated sample pretreatment workstations can replace manual processing. However, these workstations are often large, heavy, and inconvenient to transport, making them more suitable for service providers with high sample throughput. They are less suitable for end-users with limited laboratory space and low sample throughput, such as university and research institution laboratories.

[0004] More importantly, when traditional automated pipetting stations remove waste liquid, tissue samples (especially dewaxed and rehydrated tissues) have a certain probability of adhering to the outer wall of the pipette tip and being lost; while for more fragmented samples, they may be directly sucked into the pipette tip and lost. Therefore, improvements are needed for handling small quantities of precious samples. Summary of the Invention

[0005] In order to solve at least one of the technical problems mentioned in the background art, the present invention aims to provide a sample processing system.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a sample processing system, comprising: A microfluidic chip includes a reaction chamber, a first chamber connected to the reaction chamber for storing reagents, and a liquid outlet side; a filtration structure is provided between the reaction chamber and the liquid outlet side, and the waste liquid output from the reaction chamber is filtered by the filtration structure and discharged to the liquid outlet side; A positive pressure source can be selectively connected to the first chamber to provide positive pressure to force the reagents in the first chamber into the reaction chamber; A negative pressure source can be selectively connected to the liquid outlet side to provide negative pressure to drive the waste liquid output from the reaction chamber to flow to the liquid outlet side after being filtered by the filtration structure.

[0007] As an optional embodiment of the present invention, the filtration structure includes one or more filtration channels communicating with the liquid outlet side and the reaction chamber.

[0008] As an optional embodiment of the present invention, the first chamber is connected to the reaction chamber through a first flow channel; the first flow channel is at least partially bent, and / or at least partially narrowed.

[0009] As an optional embodiment of the present invention, the positive pressure source includes a positive pressure output terminal for outputting positive pressure, and the first chamber includes a first docking terminal located below the positive pressure output terminal for docking with the positive pressure output terminal; a first lifting mechanism is provided between the positive pressure output terminal and the microfluidic chip, and the microfluidic chip and the positive pressure output terminal are driven to move vertically relative to each other through the first lifting mechanism, so that the positive pressure output terminal is connected or disconnected from the first docking terminal; The negative pressure source includes a negative pressure output terminal for outputting negative pressure, and the liquid outlet side includes a second chamber capable of storing waste liquid. The second chamber includes a second docking terminal located below the negative pressure output terminal for docking with the negative pressure output terminal. The negative pressure output end can move vertically synchronously with the positive pressure output end under the drive of the first lifting mechanism, so that the negative pressure output end can be connected or disconnected from the second docking end. Alternatively, the negative pressure output terminal is connected to a second lifting mechanism, which drives the negative pressure output terminal to move vertically so that the negative pressure output terminal is connected to or disconnected from the second docking terminal.

[0010] As an optional embodiment of the present invention, the negative pressure output end and the positive pressure output end move vertically synchronously; the first lifting mechanism includes a busbar and a driving member for driving the busbar to move vertically, the busbar is provided with a first through hole constituting the positive pressure output end and a second through hole constituting the negative pressure output end.

[0011] As an optional embodiment of the present invention, the negative pressure source includes a negative pressure output terminal for outputting negative pressure; the liquid outlet side includes a liquid outlet communicating with the reaction chamber; the liquid outlet is connected to a collection unit for collecting waste liquid; the collection unit includes a third chamber for storing the waste liquid output from the liquid outlet, and the negative pressure output terminal is connected to the third chamber to create negative pressure in the third chamber; the third chamber is connected to the liquid outlet.

[0012] As an optional embodiment of the present invention, the system further includes a grinding mechanism; the grinding mechanism includes a grinding rotor, which is capable of circumferential rotation and vertical movement within the reaction chamber, and the top of the reaction chamber is open for loading the grinding rotor; the lower end of the grinding rotor constitutes a grinding end.

[0013] As an optional embodiment of the present invention, a vent hole is provided on the peripheral wall of the reaction chamber; the grinding rotor can switch vertically between a first position, a second position, and a third position within the reaction chamber; in the first position, the grinding rotor is positioned above the vent hole, so that the reaction chamber is connected to the external air pressure through the vent hole; in the second position, the grinding end of the grinding rotor is in contact with the bottom wall of the reaction chamber; in the third position, the grinding end of the grinding rotor is spaced apart from the bottom wall of the reaction chamber by a certain distance, and the grinding rotor blocks the vent hole.

[0014] As an optional embodiment of the present invention, the reaction chamber has an opening at the top, and the system further includes a hot cover that can detachably cover the opening at the top of the reaction chamber.

[0015] As an optional embodiment of the present invention, the system further includes a temperature control unit, on which the microfluidic chip is placed; the temperature control unit includes a first temperature control seat and a second temperature control seat capable of independent temperature control, the first chamber corresponding to the first temperature control seat, and the reaction chamber corresponding to the second temperature control seat; The microfluidic chip has a first temperature zone corresponding to the first temperature control seat and a second temperature zone corresponding to the second temperature control seat at its bottom; the first chamber is located in the first temperature zone and the reaction chamber is located in the second temperature zone, and the first temperature zone and the second temperature zone are at least partially isolated from each other.

[0016] As an optional embodiment of the present invention, a groove is formed at the bottom of the microfluidic chip between the first temperature zone and the second temperature zone, thereby separating the first temperature zone and the second temperature zone. Alternatively, the microfluidic chip includes a first part and a second part that are independent of each other, with the first chamber located in the first part and the reaction chamber located in the second part; and the first part is provided with a transfer chamber that connects all the first chambers; the transfer chamber is connected to the reaction chamber through a pipeline; Alternatively, the microfluidic chip includes a chip body, a first cover plate, and a second cover plate; wherein the first cover plate and the second cover plate are respectively disposed on the front and back sides of the chip body, and the first cover plate and the second cover plate are staggered in the horizontal direction; wherein a first chamber is at least partially formed between one of the first cover plate and the second cover plate and the chip body, and a reaction chamber is at least partially formed between the other of the first cover plate and the second cover plate and the chip body; Alternatively, a first temperature control seat and a second temperature control seat, one of which is located on the upper side of the microfluidic chip and the other on the lower side of the microfluidic chip, and the one located on the upper side of the microfluidic chip has a channel for vertical insertion of the first chamber or the reaction chamber.

[0017] As an optional embodiment of the present invention, the system further includes a liquid injection unit, which includes a liquid pump and an infusion end, and the microfluidic chip further includes an injection chamber, wherein the infusion end is movable relative to the microfluidic chip to connect or disconnect the injection chamber.

[0018] Compared with existing technologies, the advantages of this solution are: This invention shifts the sample reaction site from conventional experimental consumables to a microfluidic chip, allowing steps such as adding reagents, reacting, and removing waste liquid to be performed on the chip. Compared to the traditional method of using a sample processing workstation, this avoids the use of a robotic arm for liquid handling, improves the system's integration, and reduces the instrument's size.

[0019] Furthermore, in this invention, a filtration structure is provided between the reaction chamber and the liquid outlet side, so that the waste liquid output from the reaction chamber after completion will be filtered by the filtration structure before being discharged through the liquid outlet side. Through the filtration of the filtration structure, some fine sample fragments can be left in the microfluidic chip and will not be discharged with the waste liquid, thereby reducing the loss of samples when outputting waste liquid. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of one embodiment of the present invention; Figure 2 This is a schematic diagram of the microfluidic chip of the present invention; Figure 3 for Figure 2 A magnified view of the location of the intermediate reaction chamber; Figure 4 This is a schematic diagram of the bottom structure of a microfluidic chip (corresponding to Form 1 in Embodiment 1). Figure 5 This is a schematic diagram of the structure of the microfluidic chip of the present invention, which includes a first part and a second part (corresponding to form two in embodiment 1). Figure 6 for Figure 5 A schematic diagram of the internal structure viewed from below; Figure 7 This is a schematic diagram of the structure of Form 3 in Example 1; Figure 8 for Figure 7 A schematic diagram of the structure in its assembled state; Figure 9 This is a schematic diagram of the structure of Form 4 in Example 1. Figure 1 ; Figure 10 This is a schematic diagram of the structure of Form 4 in Example 1. Figure 2 ; Figure 11 This is a partial structural diagram of the fourth working state in Example 1; Figure 12 A schematic diagram of another embodiment of the present invention; Figure 13 This is a partial structural diagram of the connector and adapter of the present invention in their assembled state; Figure 14 This is a schematic diagram of the grinding unit. Figure 15 This is a schematic diagram of the grinding rotor. Figure 16 This is a schematic diagram of the grinding rotor in its first position. Figure 17 This is a schematic diagram of the grinding rotor in its second position. Figure 18 This is a schematic diagram of the grinding rotor in its third position.

[0021] Figure label: 1. Microfluidic chip; 11. Reaction chamber; 111. Vent; 12. First chamber; 121. First docking end; 13. Filter structure; 131. Filter channel; 132. Transition channel; 133. Main channel; 14. First channel; 141. Bending structure; 142. Diameter reduction structure; 15. Second chamber; 151. Second docking end; 152. First container; 16. Liquid injection chamber; 17. First temperature zone; 18. Second temperature zone; 19. Trench; 1a. Chip body 1a; 1b. First cover plate 1b; 1c. Second cover plate 1c; 2. Positive pressure source; 21. First branch; 211. First valve; 3. Negative pressure source; 31. Second branch; 32. Second valve; 4. First lifting mechanism; 41. Driving component; 42. Combustion plate; 421. First through hole; 422. Second through hole; 43. Heat cover; 44. Connecting hole; 441. Connecting pipe; 442. Fourth valve; 45. Clearance hole; 5. Sealing gasket; 51. Perforation; 6. Collection unit; 61. Third chamber; 62. Connecting pipeline; 63. Third valve; 64. Adapter; 641. Socket; 65. Connector; 651. Liquid outlet; 66. First sealing ring; 7. Injection unit; 71. Liquid pump; 72. Injection needle; 73. Processing liquid container; 74. Fourth valve; 8. Grinding mechanism; 81. Grinding rotor; 811. Keyway; 812. Raised pegs; 82. Second sealing ring; 83. Drive shaft; 831. Raised key; 84. Spring; 9. Temperature control unit; 91. First temperature control socket; 92. Second temperature control socket; 910. Channel; 911. First sealing gasket; 921. Second sealing gasket; A1, Part 1; A11, Transit Chamber; A12, Piping; A2, Part 2. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be explained and described below with reference to the accompanying drawings. However, the following embodiments are only preferred embodiments of the present invention and not all of them. Other embodiments obtained by those skilled in the art based on the embodiments in the implementation methods without creative effort are all within the protection scope of the present invention.

[0023] In the following description, terms such as “inner,” “outer,” “upper,” “lower,” “left,” and “right” that indicate orientation or positional relationship are used only for the convenience of describing the embodiments and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0024] Example 1 Please see Figure 1 As shown, this embodiment provides a sample processing system that can be used for reagent addition, reaction, and waste liquid discharge during sample processing. The system mainly includes a microfluidic chip 1, a positive pressure source 2, and a negative pressure source 3. The following is a detailed description of each component.

[0025] Combination Figure 2 As shown, the microfluidic chip 1 includes a reaction chamber 11, a first chamber 12, and a liquid outlet side.

[0026] The reaction chamber 11 is mainly used as the site for sample reaction; the first chamber 12 is mainly used for storing reagents and is connected to the reaction chamber 11.

[0027] It is understandable that, such as Figure 1 As shown, there may be one or more first chambers 12, depending on the number of reagents required to participate in the sample processing process; for example, this embodiment shows the case of using multiple first chambers 12, each of which is connected to the reaction chamber 11.

[0028] The specific structure for connecting the first chamber 12 and the reaction chamber 11 is as follows: Figure 2 As shown, a first flow channel 14 is provided between each first chamber 12 and reaction chamber 11, and the first chamber 12 is connected to the reaction chamber 11 through the first flow channel 14.

[0029] To prevent reagents in the first chamber 12 from naturally diffusing into the reaction chamber 11 via the first flow channel 14, improvements are made to the first flow channel 14 in some embodiments: like Figure 3As shown, the first flow channel 14 is designed at least partially as a bent structure 141. For example, the first flow channel 14 is continuously bent to form a region similar to an intestinal structure. The intestinal structure can increase the flow resistance of the reagent, making it difficult for the reagent to diffuse naturally into the reaction chamber 11.

[0030] In addition, such as Figure 3 As shown, in some other optional embodiments, the first flow channel 14 may be at least partially a narrowed diameter structure 142. For example, a portion of the first flow channel 14 may gradually narrow in diameter along the flow direction of the reagent (which can also be understood as the direction from the first chamber 12 to the reaction chamber 11) to form the aforementioned narrowed diameter structure 142. This method can also increase the flow resistance of the reagent, making it difficult for the reagent to diffuse naturally into the reaction chamber 11.

[0031] It is understood that, in some embodiments, the first flow channel 14 may also be designed with the aforementioned intestinal structure and the reduced diameter structure 141.

[0032] The liquid outlet side mainly serves as the waste liquid output side of the microfluidic chip 1, which can also be understood as the waste liquid output side of the reaction chamber 11. It is mainly used to discharge the waste liquid after the reaction in the reaction chamber 11 is completed.

[0033] In order to reduce the loss of fine samples during wastewater discharge, in this embodiment, as follows: Figure 2 and Figure 3 As shown, a filter structure 13 is provided between the reaction chamber 11 and the liquid outlet side. The waste liquid output from the reaction chamber 11 is filtered by the filter structure 13 and then discharged to the liquid outlet side. In this way, the waste liquid output from the reaction chamber 11 after completion will be filtered by the filter structure 13 before being discharged through the liquid outlet side. Through the filtration of the filter structure 13, some fine sample fragments can be left in the microfluidic chip 1 and will not be discharged with the waste liquid, thereby reducing the loss of samples when outputting waste liquid.

[0034] In some embodiments, the filter structure 13 may be constructed as follows: Figure 3 As shown, the filtration structure 13 includes one or more filtration channels 131 that connect the liquid outlet side and the reaction chamber 11. The filtration channels 131 act as filter holes, so that when the reaction chamber 11 discharges waste liquid to the liquid outlet side, some samples in the waste liquid cannot pass through the filtration channels 131 and remain in the microfluidic chip 1, thereby reducing sample loss.

[0035] As for the cross-sectional dimensions of the filter channel 131, they can be determined based on the size of the sample to be intercepted. Generally speaking, the cross-sectional dimensions should not be larger than those of a typical fine sample.

[0036] Among them, such as Figure 3As shown, the filter structure 13 also includes a transition channel 132 located upstream of the liquid outlet side. The filter channel 131 is located between the transition channel 132 and the reaction chamber 11 to connect the transition channel 132 and the reaction chamber 11. For example, the transition channel 132 is connected to the liquid outlet side through a main channel 133. Here, the transition channel 132 is essentially a collecting channel, used to collect the waste liquid flowing out of the filter channel 131 and then discharge it to the liquid outlet side through the main channel 133.

[0037] Positive pressure source 2 can be selectively connected to the first chamber 12 to provide positive pressure, forcing the reagent in the first chamber 12 into the reaction chamber 11. "Selectively" here means that positive pressure source 2 can be selectively connected to or not connected to the first chamber 12 depending on the processing procedure. For example, when reagent needs to be added, positive pressure source 2 is connected to the first chamber 12, and under the action of positive pressure, the reagent in the first chamber 12 is forced into the reaction chamber 11 through the high-resistance first flow channel 14. When positive pressure source 2 is not needed, it can be separated from the first chamber 12.

[0038] As an optional embodiment, the positive pressure source 2 can be selectively connected to the first chamber 12, specifically configured as follows: like Figure 1 As shown, the positive pressure source 2 includes a positive pressure output terminal for outputting positive pressure. It can be understood that the number of positive pressure output terminals is determined by the number of first chambers 12. The number of positive pressure output terminals must be able to correspond one-to-one with the number of first chambers 12, that is, one positive pressure output terminal corresponds to one first chamber 12, so as to individually control whether the reagent is output from the first chamber 12.

[0039] For example, the positive pressure source 2 includes multiple first branches 21 that correspond one-to-one with the first chamber 12. Each first branch 21 is equipped with a first valve 211 that controls the opening and closing of the first branch 21. One first branch 21 corresponds to one positive pressure output terminal.

[0040] The first chamber 12 includes a first docking end 121 located below the positive pressure output end for docking with the positive pressure output end, for example, as... Figure 1 As shown, the top of the first chamber 12 is open to serve as the first docking end 121. Specifically, the first chamber 12 extends upward at least partially beyond the top surface of the microfluidic chip 1, forming a cylindrical structure, with the first chamber 12 formed inside the cylindrical structure. In this case, the first docking end 121 can be considered as the top of the cylindrical structure.

[0041] like Figure 1 As shown, a first lifting mechanism 4 is provided between the positive pressure output terminal and the microfluidic chip 1. The microfluidic chip 1 and the positive pressure output terminal are driven to move vertically relative to each other through the first lifting mechanism 4, so that the positive pressure output terminal is connected or disconnected from the first docking terminal 121.

[0042] In some embodiments, the first lifting mechanism 4 mainly includes a manifold 42 and a driving component 41 for driving the manifold 42 to move vertically. The driving component 41 may be a cylinder, a hydraulic cylinder, a lead screw linear module, or other components, and is not specifically limited here.

[0043] The busbar 42 is located above the microfluidic chip 1, and the busbar 42 has a number of first through holes 421 corresponding to the first chamber 12. The first through holes 421 are connected to the end of the first branch 21 connected to the positive pressure source 2, so that the first through holes 421 constitute the aforementioned positive pressure output end, that is, one first through hole 421 serves as a positive pressure output end.

[0044] When it is necessary to add the reagent in the first chamber 12 into the reaction chamber 11, the manifold 42 is controlled to descend until the bottom wall of the manifold 42 abuts against the top of the cylinder constituting the first chamber 12. At this time, the lower end of the first through hole 421 (i.e., the positive pressure output end) is connected to the top of the first chamber 12 (i.e., the first docking end 121). Then, the first valve 211 and the positive pressure source 2 corresponding to the predetermined first chamber 12 are opened. Thus, under the positive pressure output by the positive pressure source 2, the reagent in the first chamber 12 is forced into the reaction chamber 11 to achieve liquid addition.

[0045] Negative pressure source 3 can be selectively connected to the liquid outlet side to provide negative pressure to drive the waste liquid output from reaction chamber 11 through filter structure 13 to flow to the liquid outlet side. The term "selectively" here can be understood in the same way as the "selectively" mentioned in the previous text about positive pressure source 2, that is, negative pressure source 3 can be selected to be connected to or disconnected from the liquid outlet side as needed.

[0046] To achieve the selective connection between negative pressure source 3 and the liquid outlet side, the specific configuration can be as follows: like Figure 1 As shown, the negative pressure source 3 includes a negative pressure output terminal for outputting negative pressure. For example, the negative pressure source 3 includes a second branch 31, and each second branch 31 is provided with a second valve 32 to control the opening and closing of the second branch 31. The second branch 31 forms a negative pressure output terminal.

[0047] The liquid outlet side includes a second chamber 15 capable of storing waste liquid, and the second chamber 15 includes a second docking end 151 located below the negative pressure output end for docking with the negative pressure output end; for example, Figure 1 As shown, the top of the microfluidic chip 1 extends upward to form a cylindrical body with an opening at the top. The interior of the cylindrical body forms part of the second chamber 15, and the top of the cylindrical body constitutes the second docking end 151.

[0048] In addition, the bottom of the microfluidic chip 1 is provided with a first container 152 for collecting waste liquid discharged from the liquid outlet side, wherein the first container 152 is connected to the second chamber 15.

[0049] The negative pressure output end can move vertically synchronously with the positive pressure output end under the drive of the first lifting mechanism 4, so that the negative pressure output end can be connected or disconnected from the second docking end 151.

[0050] The first lifting mechanism 4 has been described in detail above and can be referred to the previous description, so it will not be repeated here. In addition, a second through hole 422 is provided on the busbar 42 at the position corresponding to the second docking end 151. The second through hole 422 is connected to the second branch 31, so the second through hole 422 is equivalent to the negative pressure output end of the negative pressure source 3.

[0051] When waste liquid needs to be extracted, the first lifting mechanism 4 drives the manifold 42 to move down, so that the bottom of the manifold 42 abuts against the top of the cylinder that constitutes the second chamber 15. At this time, the second through hole 422 (i.e., the negative pressure output end) is connected to the second docking end 151, thereby realizing the docking of the second docking end 151 and the negative pressure output end. Subsequently, the second valve 32 and the negative pressure source 3 are opened. Under the action of the negative pressure source 3, a negative pressure is formed inside the first container 152 and the second chamber 15. Under the action of this negative pressure, the waste liquid extracted from the reaction chamber 11 flows into the first container 152 and is collected.

[0052] It is understood that in this embodiment, when the first through hole 421 on the busbar 42 is connected to the first docking end 121, the second through hole 422 is also connected to the second docking end 151.

[0053] Furthermore, in order to improve the sealing between the docking ends and the through holes when the busbar 42 is docked with the two docking ends (i.e., the first docking end and the second docking end), in some embodiments, such as Figure 1 As shown, a sealing gasket 5 is provided between the busbar 42 and the microfluidic chip 1. The sealing gasket 5 has through holes 51 at the positions corresponding to the first through hole 421 and the second through hole 422. The through hole 51 between the first docking end 121 and the first through hole 421 connects the first docking end 121 and the first through hole 421. The through hole 51 between the second docking end 151 and the second through hole 422 connects the second docking end 151 and the second through hole 422.

[0054] As can be seen in the aforementioned embodiments, both the positive pressure output end and the negative pressure output end are driven by the same first lifting mechanism 4 to synchronously move vertically and connect or separate from the microfluidic chip 1.

[0055] In some other alternative embodiments, a second lifting mechanism (not shown in the figure) can be set separately for the negative pressure output terminal to drive the vertical displacement of the negative pressure output terminal to achieve docking or separation. The second lifting mechanism can be a similar mechanism to the first lifting mechanism 4.

[0056] To provide suitable temperature conditions for sample reaction and reagent storage, in some embodiments, such as Figure 1 As shown, the system also includes a temperature control unit 9, and the microfluidic chip 1 is placed on the temperature control unit 9. Generally, the temperature control unit 9 is fixed on the machine base of the system, and when in use, the microfluidic chip 1 is placed on the temperature control unit 9.

[0057] To meet the different temperature requirements of sample reaction and reagent storage, in some embodiments, The temperature control unit 9 includes a first temperature control seat 91 and a second temperature control seat 92 that can independently control the temperature of each other. The first chamber 12 corresponds to the first temperature control seat 91, and the reaction chamber 11 corresponds to the second temperature control seat 92. For example, when the microfluidic chip 1 is placed on the temperature control unit 9, the first chamber 12 is above the first temperature control seat 91, and the reaction chamber 11 is above the second temperature control seat 92. In this way, the first chamber 12 and the reaction chamber 11 can be independently controlled by the first temperature control seat 91 and the second temperature control seat 92, respectively.

[0058] Furthermore, in order to reduce the temperature interaction between the first chamber 12 and the reaction chamber 11, in some embodiments, such as Figure 4 As shown, the bottom of the microfluidic chip 1 is provided with a first temperature zone 17 corresponding to the first temperature control seat 91 and a second temperature zone 18 corresponding to the second temperature control seat 92; the first chamber 12 is located above the first temperature zone 17, the reaction chamber 11 is located above the second temperature zone 18, and the first temperature zone 17 and the second temperature zone 18 are at least partially isolated from each other.

[0059] The mutual isolation between the first temperature zone 17 and the second temperature zone 18 can be any one of the following forms one to four: Form 1: such as Figure 4 As shown, a groove 19 is formed at the bottom of the microfluidic chip 1 between the first temperature zone 17 and the second temperature zone 18. The groove 19 separates the first temperature zone 17 and the second temperature zone 18. The groove 19 can increase the lateral thermal resistance to reduce the temperature interaction between the first temperature zone 17 and the second temperature zone 18.

[0060] Form two, such as Figure 5 As shown, the microfluidic chip 1 includes a first part A1 and a second part A2 that are independent of each other, that is, the first part A1 and the second part A2 are phase-separated.

[0061] All first chambers 12 are located on the first part A1, and the bottom of the first part A1 forms the first temperature zone. The reaction chamber 11 is located on the second part A2, and the bottom of the second part A2 forms the second temperature zone. In addition, the first part A1 is provided with a transfer chamber A11 that connects all the first chambers 12. The transfer chamber A11 is connected to the reaction chamber 11 through a pipe A12, so that the first temperature zone 17 and the second temperature zone 18 can also be mutually influenced.

[0062] The transfer chamber A11 mainly serves as a transfer channel. During liquid addition, the reagent in the first chamber 12 first enters the transfer chamber A11 and is then transported to the reaction chamber 11 by the pipeline A12.

[0063] Form 3: such as Figure 7 and Figure 8 As shown, the microfluidic chip includes a chip body 1a, a first cover plate 1b, and a second cover plate 1c; wherein the first cover plate 1b and the second cover plate 1c are respectively disposed on the front and back sides of the chip body 1a, and the first cover plate 1b and the second cover plate 1c are staggered in the horizontal direction; wherein the first chamber 12 is at least partially formed between one of the first cover plate 1b and the second cover plate 1c and the chip body 1a, and the reaction chamber 11 is at least partially formed between the other of the first cover plate 1b and the second cover plate 1c and the chip body 1a; the first temperature control seat 91 and the second temperature control seat are both disposed on the lower side of the chip body 1a.

[0064] For example, a first cover plate 1b is disposed at the bottom of the chip body 1a, a first chamber 12 is disposed on the chip body 1a, and the bottom of the first chamber 12 is formed between the first cover plate 1b and the chip body 1a; a reaction chamber 11 is disposed on a second cover plate 1c, the second cover plate 1c is disposed at the top of the chip body 1a, and the bottom of the reaction chamber 11 is formed between the chip body 1a and the second cover plate 1c. In this case, the bottom surface of the first cover plate 1b constitutes the first temperature zone, and the bottom surface of the chip body 1a corresponding to the position of the second cover plate 1c constitutes the second temperature zone. This is equivalent to a certain height difference between the first temperature zone and the second temperature zone in the thickness direction of the chip body 1a, which helps to increase the vertical thermal resistance and reduce the temperature influence between the two temperature zones.

[0065] In addition, a transfer chamber A11 is provided on the chip body 1a. The transfer chamber A11 is located between the first chamber 12 and the reaction chamber 11, and is connected to the first chamber 12 and the reaction chamber 11 respectively. Furthermore, the bottom of the transfer chamber A11 is formed between the first cover plate 1b and the chip body 1a.

[0066] Form 4: Combination Figure 9-11As shown, in the first temperature control socket 91 and the second temperature control socket 92, one is located on the upper side of the microfluidic chip 1, and the other is located on the lower side of the microfluidic chip 1. The one located on the upper side of the microfluidic chip 1 has a channel for vertical insertion of the first chamber 12 or the reaction chamber 11. For example: The first temperature control seat 91 is located on the upper side of the microfluidic chip 1, for example, it is fixed to the bottom of the busbar 42; the channel 910 is located in the first temperature control seat 91; the second temperature control seat 92 is located on the lower side of the microfluidic chip 1; at this time, the upper side of the microfluidic chip 1 corresponding to the first chamber 12 is equivalent to the first temperature zone, and the lower side of the microfluidic chip 1 corresponding to the reaction chamber 11 is equivalent to the second temperature zone. Thus, the first temperature zone and the second temperature zone also have a certain height difference in the vertical direction, so as to increase the vertical thermal resistance and reduce the temperature influence between the two temperature zones.

[0067] In addition, in the embodiment provided in Form 4, in order to improve the sealing performance, a first sealing gasket 911 can be provided between the first temperature control seat 91 and the microfluidic chip 1, and a second sealing gasket 92 can be provided between the top of the reaction chamber 11 and the bottom wall of the manifold 42.

[0068] In some embodiments, such as Figure 1 As shown, the system also includes a heat cover 43, which is a heating element. It can be a heat source itself or a heat-conducting component that is in direct or indirect contact with a heat source to generate heat.

[0069] The hot cover 43 can detachably cover the top opening of the reaction chamber 11; for example, the hot cover 43 is fixed at the bottom of the manifold 42 corresponding to the position of the reaction chamber 11; by moving the manifold 42 down, the hot cover 43 covers the top opening of the reaction chamber 11.

[0070] Of course, in some other alternative embodiments, a separate lifting mechanism can be provided to drive the hot cover 43 to move vertically, so as to close or open the hot cover 43 and the top opening of the reaction chamber 11.

[0071] The hot cap 43 is mainly used to reduce the condensation and liquefaction of reagents. Specifically, during the reaction process, it is necessary to form a closed space inside the reaction chamber 11 to reduce the volatilization of reagents. If the temperature of the capping part covering the top opening of the reaction chamber 11 is low, the reagents evaporated inside the reaction chamber will condense and liquefy after contacting the capping part, thus reducing the amount of reagents. Therefore, in this embodiment, the hot cap 43 is used as the capping part. Firstly, it can form a closed space inside the reaction chamber 11. Secondly, since the hot cap 43 is a heating element, the evaporated reagents are not easily condensed and liquefied even if they come into contact with the hot cap 43, thus reducing the reduction in the amount of reagents.

[0072] It is worth noting that during operation, the temperature of the hot cap 43 needs to be controlled to be no lower than the reaction temperature of the sample.

[0073] In addition, to balance the gas pressure and ensure normal positive pressure liquid addition and negative pressure waste liquid extraction, in some embodiments, such as Figure 1 As shown, the manifold 42 is provided with a connecting hole 44 at the position corresponding to the reaction chamber 11. The lower end of the connecting hole 44 passes through the heat cover 43 and the sealing gasket 5. This ensures that when the manifold 42 drives the heat cover 43 to close to the top of the reaction chamber 11, the internal space of the reaction chamber 11 can be connected to the external air pressure through the connecting hole 44, thereby achieving the purpose of balancing the air pressure.

[0074] The connecting hole 44 is connected to a connecting pipe 441, and the connecting pipe 441 is equipped with a fourth valve 442 for controlling the opening and closing of the connecting pipe. When reagents need to be added, the fourth valve 442 is opened to balance the gas pressure. During the reaction, the fourth valve 442 is closed to prevent the reagents in the reaction chamber 11 from evaporating and being lost through the connecting pipe 441.

[0075] Of course, in some other alternative embodiments, in order to balance the air pressure, a cut (not shown in the figure) can be formed directly at the top opening of the reaction chamber 11. This cut can also allow the reaction chamber 11 to be connected to the external air pressure through the cut after the hot cover 43 is closed.

[0076] Example 2 To meet the application scenarios that use a relatively large amount of processing liquid during sample processing, this embodiment further improves upon Embodiment 1 to provide a sample processing system.

[0077] like Figure 7 As shown, the sample processing system provided in this embodiment also includes a liquid injection unit 7, which includes a liquid pump 71 and an infusion end. For example, an injection needle 72 is installed on the manifold 42, with one end of the injection needle 72 connected to the outlet of the liquid pump 71 and the other end penetrating the bottom of the manifold 42; at this time, the injection needle 72 constitutes the infusion end of the liquid injection unit.

[0078] like Figure 7 As shown, the microfluidic chip 1 also includes an injection chamber 16, which is connected to the reaction chamber 11. It can be understood that the injection chamber 16 can be one of the first chambers 12, or an additional injection chamber 16 can be provided on the microfluidic chip 1, or the transfer chamber A11 provided in embodiment 1 can be used as the injection chamber 16.

[0079] The infusion end (i.e., the injection needle 72) can move vertically relative to the microfluidic chip 1 to connect or disconnect the injection chamber 16. In this embodiment, the injection needle 72 is mounted on the manifold 42 so that it moves vertically synchronously with the manifold 42.

[0080] In addition, a fourth valve 74 is provided between the infusion needle and the outlet end of the liquid pump 71; when liquid injection is required, the fourth valve 74 is opened, and the liquid pump 71 adds the treatment liquid from the treatment liquid container 73 to the injection chamber 16.

[0081] Example 3 To adapt to the waste liquid collection in scenarios involving large-scale processing of liquids, this embodiment further improves upon Embodiment 2 and provides a sample processing system.

[0082] The difference between this embodiment and Embodiments 1 and 2 is that the methods of collecting waste liquid are different. Specifically, the connection methods of the liquid outlet side and the negative pressure source 3 are different.

[0083] like Figure 7 and Figure 8 As shown, the negative pressure source 3 includes a negative pressure output terminal for outputting negative pressure; the liquid outlet side includes a liquid outlet 651 that communicates with the reaction chamber 11, preferably the liquid outlet 651 is located at the bottom of the microfluidic chip 1.

[0084] The outlet 651 is connected to a collection unit 6 for collecting waste liquid; the collection unit 6 includes a third chamber 61 for storing the waste liquid output from the outlet 651, and in some embodiments, a second container is provided to provide the third chamber 61.

[0085] The negative pressure output terminal is connected to the third chamber 61 to create a negative pressure inside the third chamber 61. For example, the negative pressure source 3 is connected to the top of the third chamber 61 through a pipeline. The third chamber 61 is connected to the liquid outlet 651, and a third valve 63 is provided between the third chamber 61 and the liquid outlet 651. When collecting waste liquid, the negative pressure source 3 and the third valve 63 are opened. Under the action of the negative pressure source 3, a negative pressure is created inside the third chamber 61. Under the action of this negative pressure, the waste liquid in the reaction chamber 11 flows into the third chamber 61 through the liquid outlet 651 and is collected by the third chamber 61.

[0086] In addition, in order to enable the collection unit 6 to be independent of the microfluidic chip 1, in some embodiments, the collection unit 6 and the microfluidic chip 1 are designed to be detachably connected, specifically: like Figure 7 As shown, the collection unit 6 also includes a connector 65 and an adapter 64 that can be detachably connected to the connector 65. For example, the adapter 64 is plugged into the connector 65. Specifically: The connector 65 is connected to the bottom of the microfluidic chip 1 and extends downward, with the liquid outlet 651 located in the connector 65.

[0087] Combination Figure 8As shown, the adapter 64 has a socket 641, wherein the socket 641 is at least partially adapted to the shape of the connector 65 for insertion of the connector 65, and the adapter 64 is connected to a connecting pipe 62, a third valve 63 is provided on the connecting pipe 62, and the socket 641 is connected to the third chamber 61 through the connecting pipe 62.

[0088] The connector 65 can be plugged into or removed from the adapter 64. When the adapter 64 is connected to the connector 65, the outlet 651 of the connector 65 can be connected to the third chamber 61 through the adapter 64 for subsequent waste liquid collection.

[0089] In some embodiments, such as Figure 8 As shown, in order to improve the sealing performance of the connector 65 and the socket 641 when they are plugged in, a first sealing ring 66 is fitted between the socket 641 of the connector 65; for example, the first sealing ring 66 is pre-fixed on the inner peripheral wall of the socket 641.

[0090] It is understood that the method and structure of the negative pressure source 3 and the waste liquid collection unit 6 provided in this embodiment can be applied not only to embodiment 2, but also to embodiment 1.

[0091] Example 4 This embodiment further provides a sample processing system based on any one of embodiments 1-3, the difference being that, Figure 7 As shown, the system provided in this embodiment also includes a grinding mechanism 8, which grinds the sample in the reaction chamber 11.

[0092] Combination Figure 9 As shown, the grinding mechanism 8 includes a grinding rotor 81, which can rotate circumferentially and move vertically within the reaction chamber 11. The top of the reaction chamber 11 is open to allow the grinding rotor 81 to be inserted. The lower end of the grinding rotor 81 forms a grinding end. For example, a plurality of protrusions 812 are provided at the lower end of the grinding rotor 81, and the protrusions 812 play a grinding role.

[0093] To improve the sealing between the grinding rotor 81 and the reaction chamber 11, such as Figure 11 and Figure 12 As shown, a second sealing ring 82 is provided between the outer peripheral wall of the grinding rotor 81 and the inner peripheral wall of the reaction chamber 11. For example, the second sealing ring 82 is fixedly sleeved on the outer peripheral wall of the grinding rotor 81.

[0094] The presence of the grinding rotor 81 makes it difficult for the reaction chamber 11 to connect with the external air pressure, thus preventing the normal addition of reagents under positive pressure and the extraction of waste liquid under negative pressure; therefore, in this embodiment: The reaction chamber 11 has ventilation holes 111 on its peripheral wall; the grinding rotor 81 can switch vertically between a first position, a second position, and a third position within the reaction chamber 11.

[0095] In the first position, such as Figure 11 As shown, the grinding rotor 81 is positioned above the vent 111 so that the reaction chamber 11 can be connected to the external air pressure through the vent 111. In particular, it is necessary to ensure that the second sealing ring 82 is positioned above the vent 111. Thus, in the first position, without the blocking effect of the grinding rotor 81 and the second sealing ring 82, the reaction chamber 11 and the external air pressure can be connected through the vent 111, thereby achieving the purpose of balancing the internal air pressure of the reaction chamber 11.

[0096] In the second position, the grinding end of the grinding rotor 81 contacts the bottom wall of the reaction chamber 11; thus, the subsequent rotation of the grinding rotor 81 can cause the grinding end (i.e., the protrusions 812) to grind the sample.

[0097] The third position is between the first and second positions. In the third position, such as... Figure 13 As shown, the grinding end (i.e., the lower end) of the grinding rotor 81 is spaced apart from the bottom wall of the reaction chamber by a certain distance, and the second sealing ring 82 is located below the vent hole to block the vent hole 111. Thus, with the grinding rotor 81 and the second sealing ring 82 blocking the vent hole 111, the internal space of the reaction chamber 11 will not be connected to the external air pressure through the vent hole 111, thereby preventing the reagent in the reaction chamber 11 from evaporating through the vent hole 111.

[0098] The specific operation process of the grinding rotor can be as follows: in the first position, a positive pressure is provided by a positive pressure source to add a reagent into the reaction chamber; then, the grinding rotor is moved to the third position to carry out the reaction process; subsequently, the grinding rotor is moved back to the first position and the waste liquid is removed by a negative pressure source; the above steps are repeated until all kinds of reactions are completed, and then the grinding rotor is moved to the second position to carry out the grinding process.

[0099] In order to control the movement of the grinding rotor 81, in some embodiments, the grinding mechanism 8 further includes a drive assembly for driving the grinding rotor 81 to rotate circumferentially within the reaction chamber 11 to grind the sample. The drive assembly is also used to drive the grinding rotor 81 to move vertically to switch between a first position, a second position, and a third position.

[0100] As an optional embodiment, the drive assembly includes a drive shaft 83 and an elastic element. The drive shaft 83 is driven to rotate by a power source, such as a motor and a linear drive module connected to the motor for driving vertical movement of the motor, such as a lead screw or a cylinder. The drive shaft 83 is connected to the main shaft of the motor and is driven to rotate circumferentially by the motor.

[0101] The grinding rotor 81 is inserted into the reaction chamber 11; the drive shaft 83 is capable of moving vertically relative to the grinding rotor 81 to generate a first state and a second state, and the drive shaft 83 is driven vertically by a linear drive module.

[0102] In the first state, such as Figure 12 and Figure 13 As shown, the drive shaft 83 is coupled to the grinding rotor 81 so that the two are relatively positioned in the circumferential direction. This circumferential relative positioning can be understood as the two being relatively stationary in the circumferential direction so that they can rotate synchronously.

[0103] In the second state, such as Figure 7 As shown, the drive shaft 83 is separated from the grinding rotor 81; the elastic element is used to provide elastic force so that when the drive shaft 83 is in the second state, it drives the grinding rotor 81 to move upward and reset.

[0104] For example, the elastic element is a spring 84, which is sleeved on the grinding rotor 81. One end of the spring 84 abuts against or is fixed to the top of the grinding rotor 81, and the other end abuts against or is fixed to the top of the reaction chamber 11.

[0105] When the drive shaft 83 moves upward into the second state, without the pressure of the drive shaft 83, the grinding rotor 81 will move upward under the force of the spring 84 to reset to the first position.

[0106] The drive shaft 83 and the grinding rotor 81 can be coupled by a keyway. For example, a protruding key 831 is provided on the peripheral wall of the drive shaft 83, and the top of the grinding rotor 81 has a keyway 811 for the protruding key 831 to be inserted. The center of the grinding rotor 81 has an insertion hole 641 for the drive shaft 83 to be inserted. When the drive shaft 83 is inserted downward into the insertion hole 641 and the protruding key 831 is engaged in the keyway 811, the drive shaft 83 and the grinding rotor 81 are positioned relative to each other in the circumferential direction, so that the drive shaft 83 rotates and drives the grinding rotor 81 to rotate, thereby achieving the grinding purpose.

[0107] When the drive shaft 83 is driven to move upward, the drive shaft 83 will gradually be pulled out of the socket 641 and eventually completely separate from the grinding rotor 81. In this way, under the action of the spring 84, the grinding rotor 81 can be reset to the first position.

[0108] Furthermore, to avoid the presence of the busbar 42 interfering with the vertical movement of the drive shaft 83, such as... Figure 7 As shown, a clearance hole 45 is provided on the busbar 42 at the position corresponding to the drive shaft 83 to allow the drive shaft 83 to pass vertically through the clearance hole 45.

[0109] Furthermore, the grinding rotor 81 is a heating element, which can be a heat source itself or a heat-conducting component that is in direct or indirect contact with a heat source to generate heat, thereby reducing reagent condensation and loss. It is understood that in this case, the heating cap 43 can be omitted, and the grinding rotor 81 can still function as the heating cap 43.

[0110] Example 5 This embodiment provides a sample processing method, including the following steps: S1. Provide a microfluidic chip 1, wherein the microfluidic chip 1 is the microfluidic chip 1 in any of the systems in Examples 1-4.

[0111] The microfluidic chip 1 mainly includes a reaction chamber 11, a first chamber 12 connected to the reaction chamber 11 for storing reagents, and a liquid outlet side; a filter structure 13 is provided between the reaction chamber 11 and the liquid outlet side, and the waste liquid output from the reaction chamber 11 is filtered by the filter structure 13 and discharged to the liquid outlet side; the filter structure 13 here can be referred to the description of the aforementioned embodiment, and will not be repeated here.

[0112] S2. Inputting reagents into reaction chamber 11: Provide positive pressure source 2 and connect it to the first chamber 12. The positive pressure provided by positive pressure source 2 drives the reagents in the first chamber 12 into the reaction chamber 11 to react.

[0113] S3. After the reaction is completed, a negative pressure source 3 is provided and connected to the liquid outlet side. The negative pressure provided by the negative pressure source 3 drives the waste liquid output from the reaction chamber 11 to flow to the liquid outlet side after being filtered by the filter structure 13.

[0114] The specific principles and steps of adding liquid from positive pressure source 2 and pumping waste liquid from negative pressure source 3 can be referred to the description of the aforementioned embodiments, and will not be elaborated further here.

[0115] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

Claims

1. A sample processing system, characterized in that, include: A microfluidic chip includes a reaction chamber, a first chamber connected to the reaction chamber for storing reagents, and a liquid outlet side; a filtration structure is provided between the reaction chamber and the liquid outlet side, and the waste liquid output from the reaction chamber is filtered by the filtration structure and discharged to the liquid outlet side; A positive pressure source can be selectively connected to the first chamber to provide positive pressure to force the reagents in the first chamber into the reaction chamber; A negative pressure source can be selectively connected to the liquid outlet side to provide negative pressure to drive the waste liquid output from the reaction chamber to flow to the liquid outlet side after being filtered by the filtration structure.

2. The sample processing system according to claim 1, characterized in that, The filtration structure includes one or more filtration channels that connect the liquid outlet side to the reaction chamber.

3. The sample processing system according to claim 1, characterized in that, The first chamber is connected to the reaction chamber via a first flow channel; the first flow channel is at least partially bent, and / or at least partially narrowed.

4. The sample processing system according to claim 1, characterized in that, The positive pressure source includes a positive pressure output terminal for outputting positive pressure, and the first chamber includes a first docking terminal located below the positive pressure output terminal for docking with the positive pressure output terminal; a first lifting mechanism is provided between the positive pressure output terminal and the microfluidic chip, and the microfluidic chip and the positive pressure output terminal are driven to move vertically relative to each other through the first lifting mechanism, so that the positive pressure output terminal is connected or disconnected from the first docking terminal; The negative pressure source includes a negative pressure output terminal for outputting negative pressure, and the liquid outlet side includes a second chamber capable of storing waste liquid. The second chamber includes a second docking terminal located below the negative pressure output terminal for docking with the negative pressure output terminal. The negative pressure output end can move vertically synchronously with the positive pressure output end under the drive of the first lifting mechanism, so that the negative pressure output end can be connected or disconnected from the second docking end. Alternatively, the negative pressure output terminal is connected to a second lifting mechanism, which drives the negative pressure output terminal to move vertically so that the negative pressure output terminal is connected to or disconnected from the second docking terminal.

5. A sample processing system according to claim 4, characterized in that, The negative pressure output end and the positive pressure output end move vertically synchronously; the first lifting mechanism includes a manifold and a driving component for driving the manifold to move vertically, the manifold is provided with a first through hole constituting the positive pressure output end and a second through hole constituting the negative pressure output end.

6. The sample processing system according to claim 1, characterized in that, The negative pressure source includes a negative pressure output terminal for outputting negative pressure; the liquid outlet side includes a liquid outlet communicating with the reaction chamber; the liquid outlet is connected to a collection unit for collecting waste liquid; the collection unit includes a third chamber for storing the waste liquid output from the liquid outlet, and the negative pressure output terminal is connected to the third chamber to create negative pressure in the third chamber; the third chamber is connected to the liquid outlet.

7. The sample processing system according to claim 1, characterized in that, The system also includes a grinding mechanism; the grinding mechanism includes a grinding rotor, which is capable of circumferential rotation and vertical movement within the reaction chamber, and the top of the reaction chamber is open to allow the grinding rotor to be inserted. The lower end of the grinding rotor forms the grinding end.

8. A sample processing system according to claim 7, characterized in that, Ventilation holes are provided on the peripheral wall of the reaction chamber; the grinding rotor can switch vertically between a first position, a second position, and a third position within the reaction chamber. In the first position, the grinding rotor is positioned above the vent hole, so that the reaction chamber is connected to the external air pressure through the vent hole; In the second position, the grinding end of the grinding rotor contacts the bottom wall of the reaction chamber; In the third position, the grinding end of the grinding rotor is separated from the bottom wall of the reaction chamber by a certain distance, and the grinding rotor blocks the vent.

9. A sample processing system according to claim 1, characterized in that, The reaction chamber has an opening at the top, and the system also includes a heat cover that can be detachably closed to the top opening of the reaction chamber.

10. A sample processing system according to claim 1, characterized in that, The system also includes a temperature control unit, on which the microfluidic chip is placed; the temperature control unit includes a first temperature control seat and a second temperature control seat that can independently control the temperature of each other, the first chamber corresponds to the first temperature control seat, and the reaction chamber corresponds to the second temperature control seat; The microfluidic chip is provided with a first temperature zone corresponding to the first temperature control socket and a second temperature zone corresponding to the second temperature control socket; the first chamber is located in the first temperature zone and the reaction chamber is located in the second temperature zone, and the first temperature zone and the second temperature zone are at least partially isolated from each other.

11. A sample processing system according to claim 10, characterized in that, The microfluidic chip has a groove formed at the bottom of the chip between the first temperature zone and the second temperature zone, which separates the first temperature zone and the second temperature zone. Alternatively, the microfluidic chip includes a first part and a second part that are independent of each other, with the first chamber located in the first part and the reaction chamber located in the second part; and the first part is provided with a transfer chamber that connects all the first chambers; the transfer chamber is connected to the reaction chamber through a pipeline; Alternatively, the microfluidic chip includes a chip body 1a, a first cover plate 1b, and a second cover plate 1c; wherein the first cover plate 1b and the second cover plate 1c are respectively disposed on the front and back sides of the chip body 1a; wherein a first chamber is at least partially formed between one of the first cover plate 1b and the second cover plate 1c and the chip body 1a, and a reaction chamber is at least partially formed between the other of the first cover plate 1b and the second cover plate 1c and the chip body 1a; Alternatively, a first temperature control seat and a second temperature control seat, one of which is located on the upper side of the microfluidic chip and the other on the lower side of the microfluidic chip, and the one located on the upper side of the microfluidic chip has a channel for vertical insertion of the first chamber or the reaction chamber.

12. A sample processing system according to claim 1, characterized in that, The system also includes a liquid injection unit, which includes a liquid pump and an infusion end. The microfluidic chip also includes an injection chamber, and the infusion end is movable relative to the microfluidic chip to connect or disconnect the injection chamber.

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