Immunochromatography detection result reading method and device based on solid quantum enhancement

Abstract

The invention provides an immunochromatography detection result reading method and device based on solid quantum enhancement, and relates to the technical field of immunochromatography detection. The immunochromatography detection result reading method comprises the following steps: emitting exciting light to a standard substance to excite the standard substance to generate a fluorescence signal for detection; microwaves are output to a standard substance, so that the standard substance generates spin resonance, and the intensity change of a fluorescence signal is caused; collecting the fluorescence signal after intensity change to obtain an optical detection magnetic resonance ODMR reference spectral line; determining a corresponding microwave calibration frequency according to the optical detection magnetic resonance ODMR reference spectral line; exciting light and microwaves which are calibrated according to the microwave calibration frequency and are subjected to amplitude or frequency modulation are emitted to a tested sample; and respectively detecting the fluorescence intensity variation of the quality control line and the detection line to obtain an immunochromatography detection result of the detected sample. The microwave frequency is calibrated by using the standard substance, the interference of background fluorescence is effectively shielded, and the detection sensitivity and accuracy are improved.

Description

Technical Field

[0001] This application relates to the field of immunochromatographic detection technology, and more specifically, to a method and apparatus for reading immunochromatographic detection results based on solid-state quantum enhancement. Background Technology

[0002] Immunochromatography is an immunoassay technique that enables rapid detection through the specific binding reaction of antigens and antibodies. Its core mechanism is to use chromatography to bind the analyte in the sample with immobilized antigens / antibodies, forming a visible or detectable signal to complete the analysis. This technique is widely used in fields such as biological detection and clinical diagnosis.

[0003] Currently, commonly used immunochromatographic techniques include colloidal gold immunochromatography and fluorescence immunochromatography. Colloidal gold immunochromatography uses colloidal gold particles as a label, and qualitative detection is achieved by observing the color development results with the naked eye. However, it suffers from poor sensitivity and difficulty in accurate quantification. Fluorescence immunochromatography uses fluorescent labels to enhance the signal, and its detection sensitivity is improved compared to the colloidal gold method. However, the nitrocellulose membrane and test strip cartridge materials in the immunochromatographic carrier emit fluorescence after stimulation, resulting in the collected fluorescence intensity not entirely coming from the fluorescent substance to be tested, ultimately affecting the accuracy of the test results.

[0004] Existing technologies also include detection methods based on diamond nitrogen-vacancy (NV) centers. Diamond NV centers are solid-state quantum materials with unique optical and spin properties. Detection is achieved by exciting NV centers to generate fluorescence and combining it with microwave resonance, which improves detection sensitivity to some extent. However, these methods do not consider the influence of environmental factors such as magnetic fields and temperature on the band gap of NV centers, which can easily lead to a mismatch between the microwave frequency and the band gap of NV centers, resulting in detection errors. Furthermore, the integration and adaptability of the detection process are poor, making it difficult to meet the detection needs of different specifications of test strips and multiple indicators. Summary of the Invention

[0005] The purpose of this application is to provide a method and device for reading immunochromatographic detection results based on solid-state quantum enhancement. It utilizes diamond NV color center standard material to achieve precise calibration of microwave frequency, and combines microwave amplitude modulation or frequency modulation with phase-locked detection technology to effectively shield background fluorescence interference, improve the sensitivity and accuracy of detection, and solve the technical problems of low sensitivity, poor quantitative effect, and susceptibility to background fluorescence interference in existing immunochromatographic detection technology, as well as the technical problems of existing quantum-based detection methods being easily affected by environmental factors and having large detection errors.

[0006] According to a first aspect of this application, at least one embodiment of this application provides a method for reading immunochromatographic detection results based on solid-state quantum enhancement. The method includes: emitting excitation light to a standard substance to excite the standard substance to generate a fluorescence signal for detection; outputting microwaves to the standard substance to induce spin resonance in the standard substance and cause an intensity change in the fluorescence signal; acquiring the fluorescence signal after the intensity change to obtain an optically detected magnetic resonance (ODMR) reference spectral line; determining a corresponding microwave calibration frequency based on the ODMR reference spectral line; emitting the excitation light and microwaves calibrated according to the microwave calibration frequency to a control line and a detection line of the sample being tested; and detecting the fluorescence intensity changes of the control line and the detection line respectively to obtain the immunochromatographic detection result of the sample being tested.

[0007] For example, in some embodiments of this application, the standard material includes diamond NV color center standard material.

[0008] For example, in some embodiments of this application, the step of outputting microwaves to the standard material to cause the standard material to generate spin resonance and cause a change in the intensity of the fluorescence signal includes: outputting microwaves using a triangular wave or sawtooth wave frequency sweep method.

[0009] For example, in some embodiments of this application, emitting the excitation light and the microwave calibrated according to the microwave calibration frequency to the control line and the detection line of the sample under test includes: amplitude modulation or frequency modulation of the microwave calibrated according to the microwave calibration frequency to cause an intensity change in the fluorescence signal of the control line and the detection line.

[0010] For example, in some embodiments of this application, the step of detecting the fluorescence intensity changes of the control line and the detection line respectively to obtain the immunochromatographic detection result of the tested sample includes: determining that the immunochromatographic detection result of the tested sample is positive when the fluorescence intensity change of the control line reaches a first preset threshold and the fluorescence intensity change of the detection line reaches a second preset threshold; determining that the immunochromatographic detection result of the tested sample is negative when the fluorescence intensity change of the control line reaches the first preset threshold but the fluorescence intensity change of the detection line does not reach the second preset threshold; and determining that the immunochromatographic process has failed when the fluorescence intensity change of the control line does not reach the first preset threshold.

[0011] According to a second aspect of this application, at least one embodiment of this application provides an immunochromatographic detection result reading device based on solid-state quantum enhancement. The immunochromatographic detection result reading device is used to execute the immunochromatographic detection result reading method as described in any one of the first aspects. The immunochromatographic detection result reading device includes: a laser for emitting excitation light to a standard substance to excite the standard substance to generate a fluorescence signal for detection; a microwave component for outputting microwaves to the standard substance, causing the standard substance to generate spin resonance and resulting in an intensity change of the fluorescence signal; a fluorescence acquisition component for acquiring the fluorescence signal after the intensity change; and a control component connected to the laser, the microwave component, and the fluorescence acquisition component, respectively, for determining an optically detected magnetic resonance (ODMR) reference spectral line based on the fluorescence signal acquired by the fluorescence acquisition component, and determining a corresponding microwave calibration frequency based on the ODMR reference spectral line. The system is configured to control the laser to emit excitation light to the control line and the detection line of the sample, and to control the microwave assembly to emit microwaves calibrated according to the microwave calibration frequency to the control line and the detection line of the sample; a phase-locked loop assembly, connected to the fluorescence acquisition assembly and the control assembly respectively, is used to extract the fluorescence intensity change of the control line and the detection line based on the fluorescence signals acquired by the fluorescence acquisition assembly; the control assembly is also used to detect the fluorescence intensity change of the control line and the detection line respectively to obtain the immunochromatographic detection result of the sample; a bracket is used to support the standard substance and the sample; and a displacement assembly, connected to the bracket and the control assembly respectively, is used to be controlled by the control assembly to move the bracket so that the laser and the microwave assembly are sequentially aligned with the control line and the detection line on the standard substance and the sample.

[0012] For example, in some embodiments of this application, the microwave component is configured to output microwaves using a triangular wave or sawtooth wave frequency sweep method when detecting the standard substance.

[0013] For example, in some embodiments of this application, the microwave component is configured to: when detecting the control line and the detection line, to perform amplitude modulation or frequency modulation on the microwave calibrated according to the microwave calibration frequency, so as to cause an intensity change in the fluorescence signal of the control line and the detection line.

[0014] For example, in some embodiments of this application, it further includes: an interaction component connected to the control component, for receiving detection instructions and transmitting them to the control component.

[0015] Through the above exemplary embodiments, the immunochromatographic detection result reading method and apparatus based on solid-state quantum enhancement provided in this application have at least one of the following beneficial effects: Improving detection accuracy and eliminating the influence of environmental factors: Precise calibration of microwave frequency is achieved through diamond NV center standard material. The microwave calibration frequency matching the current environment is determined based on the ODMR (Optically Detected Magnetic Resonance) reference spectrum. This solves the technical problem of microwave frequency mismatch caused by changes in the NV center bandgap due to environmental factors such as magnetic field and temperature. It effectively avoids detection errors and improves the accuracy of detection results. Improved detection sensitivity and shielding of background fluorescence interference: By using microwave amplitude modulation or frequency modulation combined with phase-locked detection technology, regular changes in the fluorescent signal of the marker are generated. The specific fluorescence intensity change signal is accurately extracted through the phase-locked component, effectively shielding the irregular spontaneous fluorescence interference of background substances such as nitrocellulose membrane and test strip card material. Compared with traditional fluorescence immunochromatography technology, the detection sensitivity is greatly improved, and accurate quantitative detection of the analyte can be achieved. Enhanced device adaptability to meet diverse testing needs: Automatic switching of the detection site is achieved through the displacement component, which can flexibly adapt to the control line and detection line positions of different specifications of immunochromatographic test strips. At the same time, it can realize the sequential detection of multiple detection lines on a single test strip, meet the detection needs of single and multiple indicators, and has strong scalability. Simplified detection process and easy system integration: The detection method of this application uses microwave amplitude modulation or frequency modulation to replace complex microwave sequence modulation. The components of the detection device are modularly designed and controlled by the control component. The detection process is highly automated, requiring no complex manual operation. The device selection and connection method are simple, making it easy to achieve system integration and industrial application.

[0016] It should be understood that the above general description and the following detailed description are merely exemplary and do not limit this application. Attached Figure Description

[0017] The above and other objects, features, and advantages of this application will become more apparent from the detailed description of exemplary embodiments with reference to the accompanying drawings. The drawings described below are merely some embodiments of this application and are not intended to limit the scope of this application.

[0018] Figure 1 This is a schematic diagram of the structure of the immunochromatographic test strip and holder according to an embodiment of this application; Figure 2 This is a schematic diagram of the structure of the immunochromatographic detection result reading device based on solid-state quantum enhancement according to an embodiment of this application; Figure 3This is a schematic diagram of an immunochromatographic detection result reading method based on solid-state quantum enhancement according to an embodiment of this application; Figures 4A-4D This is a schematic diagram illustrating the workflow of the immunochromatographic detection result reading method according to an embodiment of this application. Detailed Implementation

[0019] Exemplary embodiments will now be described more fully with reference to the accompanying drawings. However, these exemplary embodiments can be implemented in many forms and should not be construed as limited to the embodiments set forth herein; rather, they are provided so that this application will be thorough and complete, and will fully convey the concept of the exemplary embodiments to those skilled in the art. The same reference numerals in the drawings denote the same or similar parts, and therefore repeated descriptions of them will be omitted.

[0020] The described features, structures, or characteristics can be combined in any suitable manner in one or more embodiments. Numerous specific details are provided in the following description to give a full understanding of embodiments of this disclosure. However, those skilled in the art will recognize that the technical solutions of this disclosure can be practiced without one or more of these specific details, or other methods, components, materials, devices, etc. In these cases, well-known structures, methods, devices, implementations, materials, or operations will not be shown or described in detail.

[0021] The flowcharts shown in the accompanying drawings are merely illustrative and do not necessarily include all content and operations / steps, nor do they necessarily have to be performed in the described order. For example, some operations / steps can be broken down, while others can be combined or partially combined; therefore, the actual execution order may change depending on the specific circumstances.

[0022] The terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or apparatuses.

[0023] Those skilled in the art will understand that the accompanying drawings are merely schematic diagrams of exemplary embodiments, and the modules or processes in the drawings are not necessarily essential for implementing this application, and therefore cannot be used to limit the scope of protection of this application.

[0024] Figure 1 This is a schematic diagram of the structure of the immunochromatographic test sample and the holder according to an embodiment of this application.

[0025] like Figure 1 As shown, the bracket is used to hold the standard material and the sample to be tested. The standard material is fixed to one side of the bracket, relatively independent of the sample placement area, ensuring that the calibration and testing processes do not interfere with each other, and guaranteeing that the physical environment (magnetic field, temperature, and other environmental factors) of the calibrator and the sample to be tested is consistent. The immunochromatographic sample includes a control line (C line) and a test line (T line).

[0026] According to some embodiments, the standard material is a diamond NV color center standard material.

[0027] Diamond NV centers, composed of nitrogen atoms replacing carbon atoms in the diamond lattice and adjacent lattice vacancies, possess stable optical and spin properties. Their fluorescence signal, modulated by microwave resonance, provides a quantum enhancement basis for detection. This application selects nanodiamond NV centers as markers for immunochromatographic detection. Continuous green laser irradiation excites the nanodiamond NV centers, causing them to transition from the ground state to an excited state. Subsequently, they emit red fluorescence and return to the ground state. When microwaves of a specific frequency are applied to the nanodiamond NV centers, and this microwave frequency matches the band gap of the diamond NV centers, resonance occurs, leading to a decrease in the intensity of the red fluorescence emitted by the nanodiamond NV centers.

[0028] Figure 2 This is a schematic diagram of the structure of the immunochromatographic detection result reading device based on solid-state quantum enhancement according to an embodiment of this application.

[0029] The immunochromatographic assay result reading device includes: a laser 101, a microwave component 102, a fluorescence acquisition component 103, a phase-locked loop component 104, a control component 105, a bracket 106, and a displacement component 107.

[0030] Laser 101 is electrically connected to control component 105 and is used to emit green excitation light. The excitation light can act on the control line and detection line of the standard substance, the test sample, and the test sample, respectively, to excite the NV color center of the nanodiamond to transition from the ground state to the excited state, thereby generating a red fluorescence signal and providing a basic fluorescence source for detection.

[0031] The microwave component 102 is electrically connected to the control component 105 and is used to output microwave signals and radiate them to the measured part through an antenna.

[0032] The microwave component 102 is configured to: output microwaves using a triangular wave or sawtooth wave frequency sweep method when detecting standard substances; and to perform amplitude modulation or frequency modulation on microwaves calibrated according to microwave calibration frequency when detecting quality control lines and detection lines, so as to achieve spin resonance modulation and fluorescence signal intensity change induction.

[0033] According to some embodiments, microwave component 102 includes devices such as voltage-controlled oscillator, direct frequency synthesis (DDS), and phase-locked loop that can achieve accurate frequency microwave output.

[0034] The fluorescence acquisition component 103 is electrically connected to the control component 105. The fluorescence acquisition component 103 is used to directionally collect fluorescence signals emitted by the standard substance, control line, and detection line, and transmit them to the control component 105. The control component 105 determines the optically detected magnetic resonance (ODMR) reference spectral line based on the fluorescence signal acquired by the fluorescence acquisition component 103, and determines the microwave calibration frequency corresponding to the standard substance based on the ODMR reference spectral line.

[0035] According to some embodiments, the fluorescence acquisition component 103 includes a photodetector.

[0036] The control component 105, as the core control unit of the device, is electrically connected to the laser 101, microwave component 102, fluorescence acquisition component 103, phase-locked module 104, and displacement component 107. The functions of the control component 105 include: determining the optically detected magnetic resonance (ODMR) reference spectral line based on the fluorescence signal, and determining the microwave calibration frequency based on the ODMR reference spectral line. The control component 105 is also used to send control commands to the laser 101, microwave component 102, phase-locked module 104, and displacement component 107 to coordinate the excitation light emission, microwave output, signal acquisition, and displacement adjustment processes. The control component 105 is also used to receive the fluorescence intensity change of the test sample transmitted by the phase-locked module 104 and, in conjunction with a preset threshold, determine the immunochromatographic detection result.

[0037] According to some embodiments, the control component 105 may be a device with data processing and control functions, such as a microcontroller, DSP, FPGA, ARM, or industrial computer.

[0038] The phase-locked loop (PLL) assembly 104 is electrically connected to the fluorescence acquisition assembly 103 and the control assembly 105, respectively. The functions of the PLL assembly 104 include: extracting the regular changes in fluorescence intensity generated by the control line and the detection line under microwave amplitude modulation or frequency modulation based on the fluorescence signals corresponding to the control line and the detection line acquired by the fluorescence acquisition assembly 103, so as to shield the irregular interference of background fluorescence and provide accurate data for the determination of detection results.

[0039] According to some embodiments, the phase-locked assembly 104 may be an analog phase-locked device or a digital phase-locked device.

[0040] The bracket 106 is used to hold the standard material and the sample to be tested. The standard material is fixed to one side of the bracket and is relatively independent of the area where the sample to be tested is placed, ensuring that the calibration process and the testing process do not interfere with each other, and ensuring that the physical environment (environmental factors such as magnetic field and temperature) of the calibration material and the sample to be tested are consistent.

[0041] The displacement component 107 is connected to the bracket 106 and the control component 105 respectively, and is used to receive the control command of the control component 105 to drive the bracket 106 to move, so that the excitation light path of the laser 101 and the antenna radiation area of ​​the microwave component 102 are sequentially and accurately aligned with the standard material, the quality control line and the detection line of the sample to be tested, so as to realize the automatic switching of the detection site and adapt to different specifications of test strips and multiple index detection needs.

[0042] According to some embodiments, the displacement component 107 may employ devices capable of precise displacement, such as linear stepper motors, electric actuators, and piezoelectric ceramics.

[0043] According to an example embodiment, the immunochromatographic assay result reading device further includes an interactive component 108.

[0044] The interactive component 108 is connected to the control component 105 and is used to receive detection commands (such as start detection, parameter settings, etc.) input by the operator and transmit them to the control component 105. At the same time, the interactive component 108 can display information such as detection progress, microwave frequency calibration results, fluorescence intensity data and final detection results, so that the operator can keep track of the device's working status in real time.

[0045] According to some embodiments, the interactive component 108 may be a device for human-computer interaction, such as a touch screen, keyboard, mouse, display, or communication interface.

[0046] Figure 3 This is a schematic diagram of an immunochromatographic detection result reading method based on solid-state quantum enhancement according to an embodiment of this application.

[0047] The method for reading immunochromatographic test results includes the following steps: S201 emits excitation light to a standard substance to excite the standard substance to generate a fluorescent signal for detection.

[0048] S202 outputs microwaves to a standard substance, causing the standard substance to generate spin resonance and resulting in a change in the intensity of the fluorescence signal.

[0049] According to the example embodiment, the sample to be tested is added to the sample application area of ​​the solid-state quantum-enhanced immunochromatographic test strip. After the lateral chromatography process is completed, the test strip is placed in the sample carrying area of ​​the holder, ensuring that the control line (C line) and the detection line (T line) of the test strip are within the effective detection range. Figure 4A As shown.

[0050] The control component sends commands to the displacement component, causing the bracket to move and align the laser and microwave components with the diamond NV color center standard material on the bracket, such as... Figure 4BAs shown, the control component activates the laser to emit green excitation light, while simultaneously controlling the microwave component to output microwaves in a triangular wave or sawtooth wave frequency sweep mode. The microwave frequency covers the frequency range corresponding to the conventional bandgap of the diamond NV color center. The NV color centers in the standard material are excited and produce red fluorescence. When the microwave frequency matches the bandgap of the NV color center, the NV color center undergoes spin resonance, and the fluorescence intensity decreases significantly.

[0051] In the step of outputting microwaves to the standard material by the microwave component, the microwave component outputs microwaves using a triangular wave or sawtooth wave frequency sweep method. By sweeping the frequency with triangular waves or sawtooth waves, the matching range between the microwave frequency and the bandgap of the standard material can be fully covered, ensuring the acquisition of complete optically detected magnetic resonance (ODMR) reference spectral lines, and providing an accurate basis for subsequent microwave frequency calibration.

[0052] S203 collects fluorescence signals after intensity changes to obtain optically detected magnetic resonance (ODMR) reference spectral lines.

[0053] According to an example embodiment, the fluorescence acquisition component collects the fluorescence signal of the intensity change.

[0054] S204, determine the corresponding microwave calibration frequency based on the optically detected magnetic resonance (ODMR) reference spectral lines.

[0055] According to the example embodiment, the control component determines the optically detected magnetic resonance (ODMR) reference spectral line based on the fluorescence signal collected by the fluorescence acquisition component, analyzes the ODMR reference spectral line, and identifies the characteristic frequency of fluorescence intensity abrupt change in the spectral line. This characteristic frequency is the microwave calibration frequency that matches the NV color center bandgap under the current environment (magnetic field, temperature, etc.), thereby completing the environmental adaptability calibration of the microwave frequency.

[0056] S205 emits excitation light and microwaves calibrated according to the microwave calibration frequency to the quality control line and detection line of the sample under test, respectively.

[0057] According to the example embodiment, the control component sends a command to the displacement component, causing the displacement component to move the bracket, aligning the laser and microwave components with the control line of the test strip, such as... Figure 4C As shown. The control component controls the laser to continuously emit excitation light, while simultaneously controlling the microwave component to output microwaves calibrated according to the microwave calibration frequency and subjected to amplitude or frequency modulation. The fluorescence acquisition component collects the fluorescence signal from the quality control line and converts it into an electrical signal, while the phase-locked loop component extracts the change in fluorescence intensity and transmits it to the control component.

[0058] After the quality control line is tested, the control component sends a command to the displacement component, which then moves the bracket to align the laser and microwave components with the test line of the test strip. Figure 4D As shown, repeat the above steps to complete the acquisition of fluorescence signals and extraction of fluorescence intensity changes of the detection line.

[0059] The microwave component modulates the amplitude or frequency of the microwave calibrated according to the microwave calibration frequency, so that the microwave amplitude or frequency exhibits regular changes in intensity. This, in turn, causes the fluorescence signal of the marker (nanodiamond NV color center) at the quality control line and the detection line to produce synchronous and regular changes in intensity, creating conditions for the extraction of specific signals.

[0060] S206 was used to detect the changes in fluorescence intensity at the control line and the test line, respectively, to obtain the immunochromatographic detection results of the tested sample.

[0061] According to the example embodiment, the control component calls a preset first threshold (effective threshold for quality control line) and a second preset threshold (positive threshold for detection line), and makes a determination based on the change in fluorescence intensity: If the change in fluorescence intensity of the control line is greater than or equal to the first set threshold, and the change in fluorescence intensity of the detection line is greater than or equal to the second set threshold, it indicates that both the control line and the detection line are enriched with nanodiamond NV color center markers, and the test result of the sample is determined to be positive. If the change in fluorescence intensity of the control line is greater than or equal to the first set threshold, and the change in fluorescence intensity of the detection line is less than the second set threshold, it indicates that only the control line has marker enrichment and the detection line has no marker enrichment, and the test result of the sample is determined to be negative. If the change in fluorescence intensity of the control line is less than the first set threshold, it indicates that the immunochromatography has failed and the test result is invalid. The operator will be notified through the interactive component.

[0062] The method for reading immunochromatographic detection results based on solid-state quantum enhancement in this application is also applicable to multiple index detection.

[0063] When the sample being tested is an immunochromatographic test strip with multiple detection lines, the detection method and apparatus of this application can achieve multiple index detection in the following ways: After completing the quality control line detection, the control component sends a continuous displacement command to the displacement component according to the preset multiple detection line position parameters.

[0064] The displacement component moves the bracket sequentially, so that the laser and microwave components are aligned with the detection lines (T1 line, T2 line...Tn line) on the test strip.

[0065] For each detection line, repeat the detection process of step S206 above, and extract the fluorescence intensity change of each detection line through the phase-locked loop assembly.

[0066] The control component combines the set thresholds corresponding to each detection line to determine the detection results of each indicator, and finally outputs a comprehensive detection report of multiple indicators.

[0067] The method for reading immunochromatographic detection results based on solid-state quantum enhancement in this application is also applicable to test strips of different specifications.

[0068] When the samples being tested are immunochromatographic test strips of different specifications (with differences in the positions of the control line and the test line), adaptation can be achieved in the following ways: The operator can input the specifications of the test strip (the coordinates of the control line, test line and multiple test line) through the interactive component, or select a preset test strip specification template through the interactive component.

[0069] The control component automatically adjusts the motion trajectory and positioning accuracy of the displacement component based on the received specifications.

[0070] In the subsequent testing process, the displacement component precisely drives the bracket to move according to the instructions of the control component, so that the laser and microwave components are accurately aligned with each testing part, without the need to adjust the mechanical structure of the device, and realizes rapid adaptation of test strips of different specifications.

[0071] Through the above exemplary embodiments, the immunochromatographic detection result reading method and device based on solid-state quantum enhancement provided in this application have the following beneficial effects: Precise microwave frequency calibration is achieved using diamond NV center standard material; a microwave calibration frequency matching the current environment is determined based on ODMR reference spectral lines, effectively solving the microwave frequency mismatch problem caused by changes in the NV center bandgap due to environmental factors such as magnetic fields and temperature, thus avoiding detection errors and significantly improving the accuracy of detection results; a combination of microwave amplitude modulation or frequency modulation and phase-locked detection technology is used to create regular changes in the fluorescent signal of the marker; the specific fluorescence intensity change signal is accurately extracted through the phase-locked component, successfully shielding materials such as nitrocellulose membrane and test strip cartridge. Compared to traditional fluorescence immunochromatography, the irregular autofluorescence interference from background substances significantly improves detection sensitivity, enabling accurate detection of analytes. Automatic switching of the detection site is achieved using a displacement component, flexibly adapting to the control and detection line positions of different sizes of immunochromatographic test strips. It also supports sequential detection of multiple detection lines on a single test strip, meeting diverse detection needs for both single and multiple indicators, and offering strong scalability. The detection method uses microwave amplitude modulation or frequency modulation instead of complex microwave sequence modulation. The components of the detection device are modularly designed and uniformly coordinated and controlled by a control component, resulting in a high degree of automation in the detection process, eliminating the need for complex manual operation. Device selection and connection methods are simple, facilitating system integration and industrial application.

[0072] It should be clearly understood that this application describes how specific examples are formed and used, but this application is not limited to any details of these examples. Rather, based on the teachings of the disclosure of this application, these principles can be applied to many other embodiments.

[0073] Furthermore, it should be noted that the above figures are merely illustrative representations of the processes included in the method according to exemplary embodiments of this application, and are not intended to be limiting. It is readily understood that the processes shown in the above figures do not indicate or limit the temporal order of these processes. Additionally, it is readily understood that these processes may be executed synchronously or asynchronously, for example, in multiple modules.

[0074] Exemplary embodiments of this application have been specifically shown and described above. It should be understood that this application is not limited to the detailed structures, arrangements, or implementation methods described herein; rather, this application is intended to cover various modifications and equivalent arrangements that fall within the objectives and scope of the appended claims.

Claims

1. A method for reading immunochromatographic detection results based on solid-state quantum enhancement, characterized in that, The method for reading the immunochromatographic detection results includes: Excitation light is emitted onto a standard substance to excite the standard substance to generate a fluorescence signal for detection; Microwaves are output to the standard substance, causing the standard substance to generate spin resonance and resulting in a change in the intensity of the fluorescence signal; The fluorescence signal after intensity change is collected to obtain the optically detected magnetic resonance (ODMR) reference spectral line; The corresponding microwave calibration frequency is determined based on the optically detected magnetic resonance (ODMR) reference spectral line. The excitation light and microwaves calibrated according to the microwave calibration frequency are emitted to the quality control line and detection line of the sample under test; The changes in fluorescence intensity of the control line and the detection line are detected separately to obtain the immunochromatographic detection results of the tested sample.

2. The method for reading immunochromatographic detection results as described in claim 1, characterized in that, The standard material includes diamond NV color center standard material.

3. The method for reading immunochromatographic detection results as described in claim 1, characterized in that, The process of outputting microwaves to the standard substance, causing the standard substance to generate spin resonance, and inducing a change in the intensity of the fluorescence signal includes: Microwaves are output using a triangular wave or sawtooth wave frequency sweep method.

4. The method for reading immunochromatographic detection results as described in claim 1, characterized in that, The emission of the excitation light and the microwave calibrated according to the microwave calibration frequency to the control line and detection line of the sample under test includes: The microwave calibrated according to the microwave calibration frequency is amplitude modulated or frequency modulated to cause an intensity change in the fluorescence signal of the control line and the detection line.

5. The method for reading immunochromatographic detection results as described in claim 1, characterized in that, The step of detecting the changes in fluorescence intensity of the control line and the detection line respectively to obtain the immunochromatographic detection results of the sample includes: If the change in fluorescence intensity of the control line reaches a first set threshold and the change in fluorescence intensity of the detection line reaches a second set threshold, the immunochromatographic test result of the tested sample is determined to be positive. If the change in fluorescence intensity of the control line reaches the first set threshold, but the change in fluorescence intensity of the detection line does not reach the second set threshold, the immunochromatographic test result of the sample is determined to be negative. If the change in fluorescence intensity at the control line does not reach the first set threshold, the immunochromatography is determined to have failed.

6. A device for reading immunochromatographic detection results based on solid-state quantum enhancement, characterized in that, The immunochromatographic assay result reading device is used to perform the immunochromatographic assay result reading method as described in any one of claims 1-5, and the immunochromatographic assay result reading device comprises: A laser is used to emit excitation light onto a standard substance to excite the standard substance to generate a fluorescence signal for detection. A microwave component is used to output microwaves to the standard substance, causing the standard substance to generate spin resonance and resulting in an intensity change of the fluorescence signal; A fluorescence acquisition component is used to acquire the fluorescence signal after the intensity change; A control component, connected to the laser, the microwave component, and the fluorescence acquisition component respectively, is used to determine the optically detected magnetic resonance (ODMR) reference spectral line based on the fluorescence signal acquired by the fluorescence acquisition component, and to determine the corresponding microwave calibration frequency based on the ODMR reference spectral line. It is also used to control the laser to emit the excitation light to the control line and the detection line of the sample under test, and to control the microwave component to emit microwaves calibrated according to the microwave calibration frequency to the control line and the detection line of the sample under test. A phase-locked assembly, connected to the fluorescence acquisition assembly and the control assembly respectively, is used to extract the fluorescence intensity change of the control line and the detection line based on the fluorescence signals acquired by the fluorescence acquisition assembly. The control assembly is also used to detect the fluorescence intensity change of the control line and the detection line respectively to obtain the immunochromatographic detection result of the sample being tested. A bracket is used to support the standard substance and the sample to be tested; A displacement component, connected to the bracket and the control component respectively, is used to receive control from the control component to move the bracket so that the laser and the microwave component are sequentially aligned with the standard material, the quality control line and the detection line on the sample under test.

7. The immunochromatographic assay result reading device as described in claim 6, characterized in that, The microwave component is configured as follows: When testing the standard substance, a triangular wave or sawtooth wave frequency sweep method is used to output microwaves.

8. The immunochromatographic assay result reading device as described in claim 6, characterized in that, The microwave component is configured as follows: When testing the control line and the test line, the microwave calibrated according to the microwave calibration frequency is amplitude modulated or frequency modulated to cause an intensity change in the fluorescence signal of the control line and the test line.

9. The immunochromatographic assay result reading device as described in claim 6, characterized in that, Also includes: An interactive component, connected to the control component, is used to receive detection commands and transmit them to the control component.

Images