Test card

Abstract

The invention provides a test card which comprises a main body part, a first sealing cover part, a cover body and a dehumidification part, the main body part is provided with a sample adding cavity and a detection cavity, the surface of the main body part is provided with a first opening and a second opening, the first opening is communicated with the sample adding cavity, and the second opening is communicated with the detection cavity; the cover body can seal the first opening in an opening and closing manner; the second opening is sealed by the first sealing cover piece; wherein at least the cover body, the first sealing cover piece and the main body piece jointly form a sealed space comprising a space in the sample adding cavity and a space in the detection cavity, all the spaces in the sealed space are communicated with one another, and the dehumidification piece is located in the sealed space. According to the test card provided by the invention, the cover body is designed to be openable and closable, so that a sample can be conveniently added into the sample adding cavity, and meanwhile, a sealed space can be formed for mounting a dehumidification part, so that the consumption of the dehumidification part is saved and the assembly efficiency is improved.

Description

Technical Field

[0001] This application relates to the field of medical device technology, specifically to a test card. Background Technology

[0002] During the production and manufacturing of test cards, the cleanliness, temperature, and humidity of the production environment are strictly controlled to ensure the stability of the reagents during the production process on the test cards. Appropriate desiccants and aluminum-plastic bags are also selected for packaging. Desiccants further reduce the humidity environment inside the packaging, ensuring that the reagent performance does not significantly degrade within its shelf life. However, during the process of filling the packaging bags, it is easy for omissions or overfills to occur, and since the packaging bags are relatively large, a certain amount of desiccant is required to achieve the desired drying effect. Summary of the Invention

[0003] To address the aforementioned technical problems, this application provides a test card with low drying costs and high assembly efficiency.

[0004] One embodiment of this application provides a test card, comprising: a main body having a sample application chamber and a detection chamber, the surface of the main body having a first opening and a second opening, the first opening communicating with the sample application chamber and the second opening communicating with the detection chamber; a cover for opening and closing to seal the first opening; a first sealing member for sealing the second opening; and a dehumidifying member; wherein at least the cover, the first sealing member, and the main body together form a sealed space including the space inside the sample application chamber and the space inside the detection chamber, the spaces in the sealed space being interconnected, and the dehumidifying member being located in the sealed space.

[0005] According to one embodiment of this application, the main body is provided with a waste liquid chamber, the detection chamber and the waste liquid chamber are connected, the waste liquid chamber is used to collect excess samples, and the dehumidification component is installed in the waste liquid chamber.

[0006] According to one embodiment of this application, the main body is provided with a dehumidification chamber, the detection chamber and the dehumidification chamber are connected, and the dehumidification component is located in the dehumidification chamber.

[0007] According to one embodiment of this application, the cover is provided with a receiving cavity, the receiving cavity is connected to the sample addition cavity, and the dehumidifying element is located in the receiving cavity.

[0008] According to one embodiment of this application, the cover includes a boss, which is configured to be inserted into the sealing space when the cover seals the first opening. The boss has a receiving cavity, and the sealing space includes a space inside the receiving cavity. The dehumidifying element is installed in the receiving cavity.

[0009] According to one embodiment of this application, the cover includes a movable member and a fixed member. The boss is provided on the movable member, the fixed member is fixedly connected to the main body, the movable member is movably connected to the fixed member, and the movable member can rotate relative to the fixed member to open or close the first opening.

[0010] According to one embodiment of this application, the first open edge of the main body extends toward one side of the cover to form a sample inlet tube, the sample inlet tube has a sample inlet hole, the sample inlet hole is connected to the sample addition chamber, the cover is configured to open or close the sample inlet hole, and the boss is configured to be inserted into the sample inlet hole when the cover seals the sample inlet hole.

[0011] According to one embodiment of this application, the first open edge of the main body extends toward one side of the cover to form a sample inlet tube. One side of the cover is provided with a connecting groove, and the sample inlet tube is inserted into the connecting groove. The sample inlet tube of the main body is provided with a limiting hole around it, and the periphery of the cover is provided with a limiting part. The limiting part and the limiting hole form a snap-fit ​​engagement to install the cover onto the main body.

[0012] According to one embodiment of this application, the main body has a first side and a second side disposed opposite to each other. The first opening and the second opening are disposed on the first side. The main body is provided with a connecting channel that connects the sample application chamber and the detection chamber. The first opening and the second opening are separated by a channel wall adjacent to the connecting channel on the first side. The second side is provided with a third opening that connects to the connecting channel. The test card includes a second cap that seals the third opening. The cap, the first cap, the second cap, and the main body together form a sealed space including the space inside the sample application chamber and the space inside the detection chamber.

[0013] According to one embodiment of this application, the main body component has a groove on one side corresponding to the first surface. The groove opening forms the second opening. The first part of the groove forms the detection chamber. The second part of the groove forms the waste liquid chamber. The third part of the groove forms a buffer channel. The fourth part of the groove forms a flow channel. The fifth part of the groove forms a diversion channel. The connecting channel sequentially connects the buffer channel and the flow channel to the diversion channel. The detection chamber, the waste liquid chamber, and the diversion channel are connected. The dehumidifying component is located in the waste liquid chamber.

[0014] The test card provided in this application has a cover that can be opened and closed to seal the first opening. While facilitating the addition of samples to the sample filling chamber, it can also form a sealed space for accommodating the dehumidifier, which can effectively limit the drying range of the dehumidifier, improve the drying effect, save the amount of dehumidifier used, reduce drying costs, and eliminate the need for subsequent assembly steps of the dehumidifier, thus improving assembly efficiency. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of an embodiment of the molecular diagnostic device of this application;

[0017] Figure 2 This is a schematic diagram of the structure of an embodiment of the test card of this application;

[0018] Figure 3 yes Figure 2 The diagram shows an exploded view of the test card's structure.

[0019] Figure 4 yes Figure 2 A schematic diagram of the structure of one embodiment of the main body of the test card shown;

[0020] Figure 5 yes Figure 4 A schematic diagram of another embodiment of the main body shown;

[0021] Figure 6 yes Figure 4 A structural schematic diagram of the main component from another angle;

[0022] Figure 7 yes Figure 4 A structural schematic diagram of the main component from another angle;

[0023] Figure 8 yes Figure 4 Cross-sectional schematic diagram of the main component shown

[0024] Figure 9 yes Figure 4 A structural schematic diagram of the main component from another angle;

[0025] Figure 10 yes Figure 2 A schematic diagram of the cover of the test card shown;

[0026] Figure 11 yes Figure 10A cross-sectional schematic diagram of the cover shown;

[0027] Figure 12 yes Figure 10 The diagram shows an exploded view of the cover structure.

[0028] Figure 13 yes Figure 10 A partial structural diagram of the cover shown;

[0029] Figure 14 This is a flowchart illustrating an embodiment of the assembly method of this application;

[0030] Figure 15 This is a flowchart illustrating another embodiment of the assembly method of this application;

[0031] Figure 16 yes Figure 14 A flowchart illustrating an embodiment of step S200 of the assembly method shown;

[0032] Figure 17 yes Figure 14 A flowchart illustrating another embodiment of step S200 of the assembly method shown. Detailed Implementation

[0033] The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be particularly noted that the following embodiments are for illustrative purposes only and do not limit the scope of the application. Similarly, the following embodiments are only some, not all, embodiments of the present application, and all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of the present application.

[0034] The terms "first," "second," and "third" used in the embodiments of this application are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first," "second," or "third" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified. All directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of this application are only used to explain the relative positional relationships and movement of components in a specific posture (as shown in the figures). If the specific posture changes, the directional indication will also change accordingly. The terms "comprising" and "having," and any variations thereof, in the embodiments of this application are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or components inherent to these processes, methods, products, or devices.

[0035] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0036] This application provides a test card 10 for use in molecular diagnostic equipment to perform testing on samples loaded on the test card 10 and further process them to form diagnostic data. For example... Figure 1 As shown, molecular diagnostic equipment utilizes molecular diagnostic technology, which refers to diagnostic techniques that use nucleic acids or proteins as biomarkers for clinical testing. This provides information and decision-making support for the prediction, diagnosis, prevention, treatment, and prognosis of diseases. Especially in the face of various emerging infectious diseases, rapid and accurate molecular diagnosis is the most economical and effective measure.

[0037] The molecular diagnostic equipment may include a rack 50, a card delivery seat 20 mounted on the rack 50, a card detection seat 30 mounted on the rack 50, and a control circuit board 40 mounted on the rack 50. The card delivery seat 20 is used to hold test cards 10. The card delivery seat 20 is used for centrifuging the test cards 10. The card delivery seat 20 can slide relative to the rack 50 to transport the test cards 10 to the card detection seat 30. The card detection seat 30 generates excitation light to detect the test cards 10 and generate a detection signal. The control circuit board 40 is used to control the sliding of the card delivery seat 20 on the rack 50, control the card detection seat 30 to detect the test cards 10, receive the detection signal, and process the detection signal to generate diagnostic data.

[0038] In some embodiments, the molecular diagnostic device may further include output devices such as a display and a printer that are electrically connected to the control circuit board 40, so as to output the diagnostic data of the molecular diagnostic device through the output devices. Of course, a memory for storing diagnostic data may also be provided within the molecular diagnostic device.

[0039] Furthermore, in some embodiments, the molecular diagnostic device may also include an input device such as a display or keyboard that can be electrically connected to the control circuit board 40, so as to input control commands to the molecular diagnostic device, such as the control circuit board 40, through the input device, so as to realize the control of the molecular diagnostic device on the test card delivery seat 20 and / or the test card test seat 30 through the control circuit board 40.

[0040] Please refer to the following: Figures 2 to 4 The test card 10, also known as a molecular diagnostic centrifugal test card, is provided with a sample addition chamber 1101 and a detection chamber 1102. The sample addition chamber 1101 is used to add liquid samples, and the detection chamber 1102 is used to detect liquid samples. The molecular diagnostic device can drive the test card 10 to perform centrifugal motion. Under the action of the centrifugal field, the liquid sample in the sample addition chamber 1101 can flow to the detection chamber 1102 for detection.

[0041] In some embodiments, the sample loading chamber 1101 is also used for the pretreatment of liquid samples. The pretreatment methods may include one or more, such as chemical treatment, heat treatment, enzyme treatment, and physical separation. In some embodiments, dried reagents may be pre-loaded in the sample loading chamber 210 for the pretreatment of liquid samples. The reagents may be air-dried or oven-dried in the sample loading chamber 210, or they may be added to the sample loading chamber 1101 as lyophilized reagents.

[0042] In some embodiments, the detection chamber 1102 may be pre-loaded with detection reagents for reaction with liquid samples. The detection reagents may include solid-phase reagents, such as lyophilized bulbs. The detection reagents may also be loaded into the detection chamber 1102 in liquid form and formed into dry reagents through a drying process.

[0043] The test card 10 of this application includes a main body 110, a first sealing member 120, a cover 130, and a dehumidifying member 140. A sample addition chamber 1101 and a detection chamber 1102 are provided on the main body 110. Figure 8 As shown, the surface of the main body 110 is provided with a first opening 1013 and a second opening 1014. The first opening 1013 is connected to the sample dispensing chamber 101, and the second opening 1014 is connected to the detection chamber 1102. The cover 130 can be opened and closed to seal the first opening 1013. The first sealing member 120 seals the second opening 1014. At least the cover 130, the first sealing member 120, and the main body 110 together form a sealed space 101 including the space inside the sample dispensing chamber 1101 and the space inside the detection chamber 1102. The spaces in the sealed space 101 are interconnected. The dehumidifying member 140 is located in the sealed space 101. Optionally, the sealed space 101 is defined as being formed by at least the cover 130, the first sealing member 120, and the main body 110, and includes the space inside the sample application chamber 1101 and the space inside the detection chamber 1102. It can be understood that any space formed by the test card itself and connected to the detection chamber 1102 belongs to the sealed space 101. This definition can be understood as follows: because the sealed space 101 is not outside the test card, there is no need to design a complex, costly, and cumbersome drying method as in existing technologies; at the same time, because it is connected to the detection chamber 1102, the fundamental purpose of drying can be achieved; and at the same time, because the sealed space 101 is constituted by the test card itself, the design is simple, the style is flexible, and the effect is significant.

[0044] Optionally, the sample application chamber 1101 is connected to the first opening 1013. When the cover 130 opens the first opening 1013, the sample application chamber 1101 is connected to the outside through the first opening 1013, and the sample can be added into the sample application chamber 1101 through the first opening 1013. When the cover 130 seals the first opening 1013, the cover 130, the first sealing member 120, and the main body 110 together form a sealed space 101. The detection chamber 1102 is equipped with a detection reagent. The sample application chamber 1101 and the detection chamber 1102 are connected, and the sample can flow from the sample application chamber 1101 into the detection chamber 1102 to react with the detection reagent for detection.

[0045] Furthermore, the dehumidifying component 140 can be a desiccant. A desiccant is a substance that can remove moisture from a damp material. Desiccants can include chemical desiccants or physical desiccants. Chemical desiccants, such as calcium sulfate and calcium chloride, dry by combining with water to form hydrates; physical desiccants, such as silica gel and activated alumina, dry by physically adsorbing water. Specifically, the dehumidifying component 140 can be spherical silica gel, with a diameter of 2mm to 5mm, for example, 2mm, 3mm, 3.5mm, 5mm, or any value between these values.

[0046] In some embodiments, the dehumidifier 140 is a relatively independent material. The dehumidifier 140 can have its own independent packaging or be a bare solid. The dehumidifier 140 can be used to reduce humidity and minimize reagent performance degradation. In the existing test card 10 packaging process, a step of "filling the packaging bag with desiccant" is required. During automated production, there are instances of missed or overfilled desiccant. Missed desiccant affects reagent performance, while overfilling wastes material costs and is difficult to detect before and after sealing. During transportation, the packaging bag may be squeezed, causing the desiccant to break and leak into the packaging. Leaked desiccant powder / particles can affect reagent performance and directly lead to product failure. The dehumidifier 140 in this application is built into the test card 10, eliminating the need for the "filling the packaging bag with desiccant" step, saving time and production costs. Simultaneously, the volume of the sealed space 101 formed on the test card 10 is much smaller than the space of the sealed bag, reducing the drying range of the dehumidifier 140 and thus saving the amount of dehumidifier 140 used.

[0047] Furthermore, the test card 10 can be made of rigid plastic or metal alloy material, so that the dehumidifier 140 built into the test card 10 can be prevented from being crushed. In some other embodiments, the test card 10 can be provided with a corresponding rigid structure as needed to accommodate the dehumidifier 140.

[0048] Specifically, the main body 110 can be made of materials such as ABS (Acrylonitrile Butadiene Styrene Plastic), PDMS (Polydimethylsiloxane), PC (Polycarbonate), PMMA (Polymethyl methacrylate), PS (General Purpose Polystyrene), PP (Polypropylene), COC (copolymers of cycloolefin), or COP (Cyclo-Olefin Polymer), and can be processed by injection molding, CNC machine tool processing, or 3D printing.

[0049] In some embodiments, the first sealing member 120 may be a sealing film, and the first sealing member 120 may be made of materials such as compacting adhesive, UV-curable adhesive, or optical-grade double-sided adhesive, or it may be made of a material similar to the main body member 110. The first sealing member 120 and the main body member 110 may be sealed by means of, for example, ultrasonic welding, laser welding, or adhesive sealing.

[0050] Optionally, the dehumidifier 140 can be installed on the main body 110 or the cover 130. Specifically, the dehumidifier 140 can be installed at any position on the main body 110 or the cover 130, as long as the space where the dehumidifier 140 is located is in communication with the detection chamber 1102, and the dehumidifier 140 can adsorb and dry the moisture in the detection chamber 1102.

[0051] Optionally, the main body 110 is provided with a waste liquid chamber 1103. The detection chamber 1102 and the waste liquid chamber 1103 are connected. The sealed space 101 includes the space inside the sample addition chamber 1101, the space inside the detection chamber 1102, and the space inside the waste liquid chamber 1103. The waste liquid chamber 1103 is used to collect excess samples, and the dehumidifier 140 is installed in the waste liquid chamber 1103. Using centrifugation technology, the liquid sample added to the sample addition chamber 1101 can be guided to the detection chamber 1102 for detection. After the detection chamber 1102 is filled, excess liquid sample can flow to the waste liquid chamber 1103 for collection. The dehumidifier 140 is housed in the waste liquid chamber 1103, enabling the reuse of the waste liquid chamber 1103. This allows the waste liquid chamber 1103 to both house the dehumidifier 140 and collect waste liquid. Simultaneously, it avoids the need to specifically create a cavity on the main body 110 to house the dehumidifier 140, simplifying the manufacturing process.

[0052] In some embodiments, the waste liquid chamber 1103 can be a tank structure, the second opening 1014 includes the opening of the waste liquid chamber 1103, and the first sealing member 120 can seal the opening of the waste liquid chamber 1103. In some other embodiments, the waste liquid chamber 1103 can be a cavity structure disposed within the main body 110, and the waste liquid chamber 1103 is not directly connected to the outside.

[0053] Furthermore, the direction from the waste liquid chamber 1103 to the detection chamber 1102 can be opposite to the rotation direction of the test card 10, so that the sample flowing from the sample application chamber 1101 to the detection chamber 1102 first fills the detection chamber 1102 and then fills the waste liquid chamber 1103. For example, if the test card 10 rotates clockwise, the direction from the waste liquid chamber 1103 to the detection chamber 1102 is counterclockwise. In some other embodiments, without affecting the normal use of the test card 10, the dehumidifier 140 can also be built into the detection chamber 1102 or the sample application chamber 1101. For example, there can be multiple detection chambers 1102, and the dehumidifier 140 can be located in the detection chamber 1102 at the foremost point along the rotation direction of the test card 10.

[0054] In some embodiments, such as Figure 5 As shown, the main body 110 is provided with a dehumidification chamber 1104, the detection chamber 1102 and the dehumidification chamber 1104 are connected, the sealed space 101 includes the space inside the sample addition chamber 1101, the space inside the detection chamber 1102, the space inside the waste liquid chamber 1103 and the space inside the dehumidification chamber 1104, and the dehumidification component 140 is located in the dehumidification chamber 1104.

[0055] In some embodiments, the dehumidification chamber 1104 can be a groove structure, the second opening 1014 includes the groove of the dehumidification chamber 1104, and the first cover 120 can seal the groove of the dehumidification chamber 1104. In some other embodiments, the dehumidification chamber 1104 can be a cavity structure disposed within the main body 110, and the dehumidification chamber 1104 is not directly connected to the outside.

[0056] Optionally, such as Figure 6 and Figure 7 As shown, the main body 110 has a first surface 112 and a second surface 113 arranged opposite to each other. A first opening 1013 and a second opening 1014 are provided on the first surface 112. The main body 110 is provided with a connecting channel 1015, which connects the sample application chamber 1101 and the detection chamber 1102.

[0057] Optionally, the main body 110 has a groove 1107 on the side corresponding to the first surface 112. The groove opening of the groove 1107 forms a second opening 1014. The first part of the groove 1107 forms a detection chamber 1102. The second part of the groove 1107 forms a waste liquid chamber 1103. The third part of the groove 1107 forms a buffer channel 1016. The fourth part of the groove 1107 forms a flow channel 1018. The fifth part of the groove 1107 forms a diversion channel 1017. The connecting channel 1015 sequentially connects to the diversion channel 1017 through the buffer channel 1016 and the flow channel 1018. The detection chamber 1102, the waste liquid chamber 1103 and the diversion channel 1017 are connected. The dehumidifying component 140 is located in the waste liquid chamber 1103.

[0058] In some embodiments, the number of detection cavities 1102 can be one or more, for example, the number of detection cavities 1102 can be 1, 2, 4, 5, 8, etc. Multiple detection cavities 1102 can be arranged sequentially along the rotation direction of the test card 10 and all of them are connected to the diversion channel 1017.

[0059] In some embodiments, such as Figure 3 , Figure 8 and Figure 9 As shown, the first opening 1013 and the second opening 1014 are separated by a channel wall 116 adjacent to the connecting channel 1015 of the first surface 112. The second surface 113 has a third opening 1019, which communicates with the connecting channel 1015. The test card 10 includes a second cover 150 that seals the third opening 1019. The cover 130, the first cover 120, the second cover 150, and the main body 110 together form a sealed space 101 including the space inside the sample filling chamber 1101 and the space inside the detection chamber 1102.

[0060] Furthermore, the second cover 150 can be a sealing film, and the second cover 150 can be fixed to the second surface 113 by means of bonding, hot pressing, etc.

[0061] In some embodiments, the connecting channel 1015 may also be built into the main body 110, with both ends of the connecting channel 1015 communicating with the sample filling cavity 1101 and the groove 1107, respectively. The second surface 113 does not have a third opening 1019 communicating with the connecting channel 1015, thus eliminating the need for a second sealing member 150 for sealing. In some other embodiments, the connecting channel 1015 may also be located on the first surface 112, with the second opening 1014 communicating with the connecting channel 1015, allowing the first sealing member 120 to seal the connecting channel 1015. In this case, only the cover 130, the first sealing member 120, and the main body 110 are needed to form a sealed space 101.

[0062] Optionally, such as Figure 4As shown, the main body 110 can be a plate-like structure. The main body 110 can generally be fan-shaped, specifically a fan ring, fan blade, or disc shape. For example, the main body 110 can be a fan shape formed by connecting two straight sides and one arc-shaped side end to end, or a fan ring formed by connecting one straight side, one outer arc-shaped side, one straight side, and one inner arc-shaped side end to end. The main body 110 can also be other shapes, such as triangles or trapezoids, which will not be elaborated here. The main body 110 can include a first arc-shaped side 114 and a second arc-shaped side 115. The first arc-shaped side 114 and the second arc-shaped side 115 have the same center. The radius of the first arc-shaped side 114 is larger than the radius of the second arc-shaped side 115. The two ends of the first arc-shaped side 114 and the two ends of the second arc-shaped side 115 are connected by straight sides to form a fan ring. The detection chamber 1102 is positioned near the first arc-shaped edge 114, and the sample application chamber 1101 is positioned near the second arc-shaped edge 115. Multiple detection chambers 1102 can be arranged sequentially along the first arc-shaped edge 114, and the shape of the diversion channel 1017 can also be an arc with the same center as the first arc-shaped edge 114. The flow channel 1018 can be connected to the rearmost end of the diversion channel 1017 along the rotation direction of the test card 10. For example, when the test card 10 rotates clockwise along a pivot axis passing through the center of the first arc-shaped edge 114 and perpendicular to the first surface 112, with the first arc-shaped edge 114 as the bottom and the second arc-shaped edge 115 as the top, the flow channel 1018 is connected to the rightmost end of the diversion channel 1017, while the waste liquid chamber 1103 is located to the left of the detection chamber 1102. The dehumidifier 140 can be located in the waste liquid chamber 1103, or the dehumidifier 140 can be located in the leftmost detection chamber 1102.

[0063] Optionally, such as Figure 10 and Figure 11 As shown, the cover 130 has a receiving cavity 1301, which is connected to the sample application cavity 1101. The dehumidifying component 140 is located in the receiving cavity 1301. The sealed space 101 includes the space inside the receiving cavity 1301. When the cover 130 seals the first opening 1013, the receiving cavity 1301 is connected to the detection cavity 1102, and the dehumidifying component 140 inside the receiving cavity 1301 can dry the sealed space 101.

[0064] Furthermore, the sealing space 101 may include all the communicating spaces inside the main body 110. In some other embodiments, the sealing space 101 may also be a part of the space inside the main body 110, including the space inside the detection cavity 1102. For example, the sealing space 101 may include the space inside the detection cavity 1102, and one of the spaces including the space inside the waste liquid cavity 1103, the space inside the dehumidification cavity 1104, the space inside the receiving cavity 1301, etc., or include a space formed by any combination of the above-mentioned multiple cavities.

[0065] In some embodiments, the cover 130 includes a boss 1311, which is configured to be inserted into the sealing space 101 when the cover 130 seals the first opening 1013. The boss 1311 has a receiving cavity 1301 in which the dehumidifier 140 is installed.

[0066] In some embodiments, the cover 130 is detachably connected to the main body 110. The cover 130 can be detachably connected to the main body 110 by means of threaded connection, snap-fit, etc. For example, the cover 130 can be snapped to the main body 110 by a snap-fit ​​structure to seal the first opening 1013; when it is necessary to open the first opening 1013, the cover 130 and the main body 110 can be disengaged to remove the cover 130 so that the sample filling chamber 1101 can communicate with the outside.

[0067] Furthermore, the cover 130 includes a movable member 131 and a fixed member 132. A boss 1311 is provided on the movable member 131. The fixed member 132 is fixedly connected to the main body 110. The movable member 131 is movably connected to the fixed member 132. The movable member 131 can rotate relative to the fixed member 132 to open or close the first opening 1013.

[0068] Specifically, a connector 133 connects the movable part 131 and the fixed part 132, forming a flip-top structure. The connector 133 can be a hinge, allowing the movable part 131 and the fixed part 132 to be connected. The movable part 131 can rotate relative to the fixed part 132. A latch can be provided on the side of the movable part 131 away from the connector 133, and a slot can be provided on the side of the fixed part 132 away from the connector 133. The movable part 131 and the fixed part 132 can form a snap-fit ​​engagement when the cover 130 is closed. The cover 130 can be made of a plastic with a certain degree of elasticity, allowing the latch of the movable part 131 to undergo elastic deformation to achieve the switching between the open and closed states of the cover 130.

[0069] In some other embodiments, the movable member 131 and the fixed member 132 may also be detachably connected. Further, the movable member 131 and the fixed member 132 may be detachably connected by means of threaded connection, snap-fit, or other methods. For example, the movable member 131 may be a groove structure, with its inner wall thread engaging with the outer wall thread of the fixed member 132. When the cover 130 is in the closed state, the movable member 131 can be fixed to the fixed member 132 by threaded connection to seal the sample filling chamber 1101. When the cover 130 opens the first opening 1013, the movable member 131 can be detached from the fixed member 132, allowing the sample filling chamber 1101 to communicate with the outside.

[0070] Optionally, such as Figure 6As shown, the edge of the first opening 1013 of the main body 110 extends toward the cover 130 to form a sample inlet tube 111. The sample inlet tube 111 has a sample inlet hole 1105, which is connected to the sample addition chamber 1101. The cover 130 is configured to open or close the sample inlet hole 1105. The boss 1311 is configured to be inserted into the sample inlet hole 1105 when the cover 130 seals the sample inlet hole 1105.

[0071] In some embodiments, the movable member 131 may rotate relative to the fixed member 132 to open or close the inlet port 1105. The boss 1311 is configured to be inserted into the inlet port 1105 when the movable member 131 closes the inlet port 1105.

[0072] In some embodiments, one side of the cover 130 is provided with a connecting groove (not shown in the figure), the sample inlet tube 111 is inserted into the connecting groove, the sample inlet tube 111 of the main body 110 is provided with a limiting hole 1106 around it, and the periphery of the cover 130 is provided with a limiting part 1321. The limiting part 1321 and the limiting hole 1106 form a snap-fit ​​engagement to install the cover 130 onto the main body 110.

[0073] Furthermore, such as Figure 9 As shown, the fixing member 132 of the cover 130 has a through hole 1302. The movable member 131 can cover one end of the through hole 1302 to form a connecting groove, and the sample inlet tube 111 is inserted into the through hole 1302. The fixing member 132 has a limiting part 1321, which is arranged around the through hole 1302. The limiting part 1321 and the limiting hole 1106 form a snap-fit ​​engagement to fix the fixing member 132 to the main body 110. The boss 1311 is inserted into the sample inlet hole 1105 when the movable member 131 covers the through hole 1302, and is pulled out of the sample inlet hole 1105 when the movable member 131 opens the through hole 1302.

[0074] Specifically, the number of limiting holes 1106 can be multiple and arranged at intervals around the sample inlet tube 111. For example, the number of limiting holes 1106 can be three. In some other embodiments, the fastener 132 can also be fixed to the main body 110 by means of bonding, welding, screw connection or threaded connection, etc., which will not be described in detail here.

[0075] Furthermore, such as Figures 11 to 13 As shown, a filter element 1312 can be provided on the boss 1311. The shape of the receiving cavity 1301 can be annular around the filter element 1312. The filter element 1312 can block the escape of pollutants such as aerosols and biomolecules in the test card 10, and can also prevent external substances from entering the sealed space 101 and contaminating the reagent.

[0076] Furthermore, the boss 1311 is provided with a filter groove 1303, and the receiving cavity 1301 is arranged around the filter groove 1303. The bottom of the filter groove 1303 is provided with an air inlet 1304, which communicates with the filter groove 1303. The filter element 1312 is inserted into the filter groove 1303. When the cover 130 is closed, the air inlet 1304 is located on the side of the boss 1311 facing the sample filling cavity 1101, and the air inlet 1304 communicates with the sample filling cavity 1101 when the cover 130 is closed.

[0077] Optionally, the movable component 131 is provided with a fourth opening 1305, which is located on the side of the movable component 131 away from the air inlet 1304. The fourth opening 1305 communicates with the receiving cavity 1301 and the filter tank 1303. The dehumidifier 140 can be installed into the receiving cavity 1301 through the fourth opening 1305, and the filter 1312 can be installed into the filter tank 1303 through the fourth opening 1305. The movable component 131 includes a third cover 134, which covers the fourth opening 1305.

[0078] Furthermore, the boss 1311 is provided with a first wall 1313, which forms a filter groove 1303. The third cover 134 is provided with a second wall 1341, which forms a positioning groove 1308. The first wall 1313 is inserted into the positioning groove 1308. The first wall 1313 is provided with a first notch 1306, and the filter groove 1303 is connected to the receiving cavity 1301 through the first notch 1306. The side wall of the receiving cavity 1301 is provided with a second notch 1307 near the connector 133. The receiving cavity 1301 can be connected to the outside through the second notch 1307. The second notch 1307 is used to adjust the internal air pressure of the test card 10. During the production or testing of the test card 10, there may be a heating process, which may cause the internal air pressure to rise. When the air pressure in the sealed space 101 is too high, the gas can be discharged from the second notch 1307.

[0079] Specifically, the third cover 134 can be fixed to the movable part 131 by welding, bonding, snap-fitting, or other methods.

[0080] This application also provides an assembly method, such as... Figure 14 As shown, the assembly method is used to assemble the test card 10 of the above embodiment. The assembly method includes:

[0081] Step S200: Install the dehumidifier 140 and the test reagent.

[0082] The detection reagent is contained in the detection chamber 1102. The detection reagent may include a solid-phase reagent, such as lyophilized bulbs. Solid-phase fixation refers to immobilizing the reagent on the surface of a specific solid medium through adsorption and fixation. The detection reagent may also include dry reagents, which include primers used in the amplification reaction and one or more of the following: DNA (deoxyribonucleic acid) binding dyes, enzymes, magnesium sulfate, potassium chloride, and dNTPs (nucleoside triphosphates). The dry reagent may be introduced into the detection chamber 1102 in a liquid state and then dried to form a dry reagent.

[0083] Step S500: Seal the test card 10 into a bag.

[0084] In the existing assembly process, the dehumidifier 140 is located outside the test card 10. After installing the test reagent and placing the test card 10 into an aluminum-plastic bag, the dehumidifier 140 must also be placed into the aluminum-plastic bag and then sealed. The entire process is time-consuming, requiring continuous operation from reagent loading to sealing, and placing strict requirements on the temperature and humidity of the production environment. This application installs the dehumidifier 140 and the test reagent onto the test card 10 in the same process. The dehumidifier 140 is installed simultaneously with the test reagent being loaded into the detection chamber 1102 of the main component 110. For example, the desiccant can be placed into the waste liquid chamber 1103, eliminating the "desiccant bagging" step. After the test card 10 is sealed, the sealed space 101 formed by the test card 10 is very small, and the entrance and exit for exchange with the external environment are also small, forming a relatively enclosed space. The desiccant reduces the humidity within this space to a very low level, isolating the subsequent production environment. Therefore, the requirements for temperature and humidity in the production environment can be appropriately reduced, saving energy. This also prevents subsequent processes from affecting earlier processes, making production arrangements more flexible.

[0085] Furthermore, such as Figure 15 As shown, the assembly method may include:

[0086] Step S100: Cover the flow channel 1018 with the second cover 150.

[0087] The second sealing member 150 can be pressed against the second surface 113. The flow channel 1018 connecting the sample dispensing chamber 1101 and the detection chamber 1102 is provided on the second surface 113 of the main body 110, which facilitates the separate sealing of the sample dispensing chamber 1101 and the detection chamber 1102, thereby improving the sealing effect. In some embodiments, after the second sealing member 150 is pressed against the second surface 113, the process of pressing a label may also be included.

[0088] Step S200: Install the dehumidifier 140 and the test reagent.

[0089] Step S300: Cover the second opening 1014 with the first cover 120.

[0090] Step S400: Cover the first opening 1013 with the cover 130.

[0091] The detection chamber 1102 can be connected to the outside through the second opening 1014, and the sample addition chamber 1101 can be connected to the outside through the first opening 1013. The first sealing member 120 can be pressed against the first surface 112 to seal the second opening 1014. The cover 130 can be closed to seal the first opening 1013, so that the sealed space 101 is not connected to the outside, thereby reducing the drying range of the dehumidifying member 140.

[0092] Step S500: Seal the test card 10 into a bag.

[0093] Furthermore, such as Figure 16 As shown, step S200 may include:

[0094] Step S210: Install the dehumidifier 140 and the test reagent onto the main body 110;

[0095] Step S220: Determine the installation status of the dehumidifier 140 through optical imaging analysis.

[0096] The solidification process of the detection reagent can be carried out by spotting liquid reagents, drying, or loading lyophilized reagents. This process, along with the loading of the desiccant, can be combined into a single production step. These steps can be integrated into the assembly process of the test card 10, avoiding frequent loading and unloading and shortening assembly time. The first surface 112 of the main component 110 can simultaneously house the dehumidifier 140 and the detection reagent. For example, the dehumidifier 140 can be installed in the waste liquid chamber 1103, and the detection reagent can be installed in the detection chamber 1102. During automated assembly, the presence and overloading of the dehumidifier 140 can be detected using optical imaging or other methods, preventing omissions or overloading. Specifically, the presence and overloading of the dehumidifier 140 can be determined by analyzing the image information of the first surface 112.

[0097] In some other embodiments, such as Figure 17 As shown, the test reagent and the dehumidifier 140 can be installed separately, and step S200 may include:

[0098] Step S230: Install the test reagent onto the main body 110.

[0099] Step S240: Install the dehumidifier 140 onto the cover 130.

[0100] The dehumidifier 140 is installed in the receiving cavity 1301 of the cover 130. After the cover 130 is sealed to the first opening 1013 in step S400, the cover 130, the first sealing member 120, the main body 110, and the second sealing member 150 together form a sealed space 101 including the space inside the sample dispensing cavity 1101, the space inside the detection cavity 1102, the space inside the receiving cavity 1301, and the space inside the connecting channel 1015. The dehumidifier 140 can dry the sealed space 101. The dehumidifier 140 can also be pre-installed on the cover 130 during the assembly of the cover 130. For example, the dehumidifier 140 can be installed on the cover 130 together with the filter 1312 in the same process.

[0101] The test card 10 provided in this application has a cover 130 that can be opened and closed to seal the first opening 1013. While facilitating the addition of samples to the sample filling chamber 1101, it can also form a sealed space 101 for accommodating the dehumidifier 140. This can effectively limit the drying range of the dehumidifier 140, improve the drying effect, and help save the amount of dehumidifier 140 used. At the same time, it eliminates the need for subsequent assembly steps of the dehumidifier 140. The integration of the test card 10 assembly process is improved, which helps to shorten the assembly time, improve the assembly efficiency, and appropriately relax the temperature and humidity requirements of the production environment, thus saving energy.

[0102] The above description is only a part of the embodiments of this application and does not limit the scope of protection of this application. Any equivalent device or equivalent process transformation made based on the content of this application specification and drawings, or direct or indirect application in other related technical fields, are similarly included in the patent protection scope of this application.

Claims

1. A test card, characterized in that, include: The main body has a sample dispensing chamber and a detection chamber. The surface of the main body has a first opening and a second opening. The first opening is connected to the sample dispensing chamber, and the second opening is connected to the detection chamber. The cover can be opened and closed to seal the first opening; The first sealing element seals the second opening; Dehumidifiers; Wherein, at least the cover, the first sealing member, and the main body member together form a sealed space including the space inside the sample addition chamber and the space inside the detection chamber, and the spaces in the sealed space are interconnected, and the dehumidifying member is located in the sealed space.

2. The test card according to claim 1, characterized in that, The main body is provided with a waste liquid chamber, the detection chamber and the waste liquid chamber are connected, the waste liquid chamber is used to collect excess samples, and the dehumidification component is installed in the waste liquid chamber.

3. The test card according to claim 1, characterized in that, The main body is provided with a dehumidification chamber, the detection chamber and the dehumidification chamber are connected, and the dehumidification component is located in the dehumidification chamber.

4. The test card according to claim 1, characterized in that, The cover has a receiving cavity, which is connected to the sample addition cavity, and the dehumidifying element is located in the receiving cavity.

5. The test card according to claim 1, characterized in that, The cover includes a boss configured to be inserted into the sealing space when the cover seals the first opening. The boss has a receiving cavity, and the sealing space includes a space inside the receiving cavity. The dehumidifying element is installed in the receiving cavity.

6. The test card according to claim 5, characterized in that, The cover includes a movable part and a fixed part. The boss is provided on the movable part. The fixed part is fixedly connected to the main body. The movable part is movably connected to the fixed part. The movable part can rotate relative to the fixed part to open or close the first opening.

7. The test card according to claim 5, characterized in that, The first open edge of the main body extends toward one side of the cover to form a sample inlet tube. The sample inlet tube has a sample inlet hole, which is connected to the sample dispensing chamber. The cover is configured to open or close the sample inlet hole. The boss is configured to be inserted into the sample inlet hole when the cover seals the sample inlet hole.

8. The test card according to claim 5, characterized in that, The first open edge of the main body extends toward one side of the cover to form a sample inlet tube. One side of the cover is provided with a connecting groove, and the sample inlet tube is inserted into the connecting groove. The sample inlet tube of the main body is provided with a limiting hole around it, and the periphery of the cover is provided with a limiting part. The limiting part and the limiting hole form a snap-fit ​​engagement to install the cover onto the main body.

9. The test card according to claim 1, characterized in that, The main body has a first side and a second side arranged opposite to each other. The first opening and the second opening are located on the first side. The main body has a connecting channel that connects the sample application chamber and the detection chamber. The first opening and the second opening are separated by a channel wall adjacent to the connecting channel on the first side. The second side has a third opening that connects to the connecting channel. The test card includes a second cap that seals the third opening. The cap, the first cap, the second cap, and the main body together form a sealed space including the space inside the sample application chamber and the space inside the detection chamber.

10. The test card according to claim 9, characterized in that, The main body has a groove on one side corresponding to the first surface. The groove opening forms the second opening. The first part of the groove forms the detection chamber. The second part of the groove forms the waste liquid chamber. The third part of the groove forms the buffer channel. The fourth part of the groove forms the flow channel. The fifth part of the groove forms the diversion channel. The connecting channel sequentially connects the buffer channel and the flow channel to the diversion channel. The detection chamber, the waste liquid chamber, and the diversion channel are connected. The dehumidifying component is located in the waste liquid chamber.

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