Sample adding device and method, sample analyzer and method

Abstract

The application discloses a sample adding device and a sample adding method thereof, a sample analyzer and a sample adding method thereof. The sample adding device comprises a sampling needle, a first driving member, a second driving member and a controller. The controller is configured to control the first driving member to move the sampling needle to a liquid container, control the second driving member to draw liquid from the liquid container after the liquid in the liquid container is higher than a needle opening of the sampling needle, control the first driving member to move the needle opening of the sampling needle away from a liquid surface of the liquid container, control the second driving member to draw a preset volume of gas, control the first driving member to move the sampling needle to an empty cup container, and control the second driving member to discharge a liquid drop from the sampling needle when a distance between the needle opening of the sampling needle and a bottom wall of the empty cup container is within a preset range, and the speed of the liquid drop reaching the needle opening of the sampling needle is greater than 0. The sample adding device can improve the accuracy and repeatability of sample adding, and can also improve the efficiency of sample adding.

Description

Technical Field

[0001] This application relates to the field of medical device technology, and in particular to a sample application device and its application method, and a sample analyzer and its application method. Background Technology

[0002] Currently, sample analysis instruments are widely used in fields such as chemistry, life sciences, pharmaceuticals, and the environment, and accuracy and repeatability are important indicators for evaluating the performance of analytical instruments.

[0003] During operation, sample analysis instruments often require the addition of samples to empty containers. When adding small-volume samples, it is necessary to prevent the sample from adhering to the tip of the sampling needle and being unable to be expelled.

[0004] In the prior art, when small droplets are drained, the droplets are easily affected by the adhesion force of the liquid on the outer surface of the sampling needle at the needle tip, and they wet and spread along the outer surface of the sampling needle, thus causing the draining to fail. Summary of the Invention

[0005] This application provides a sample dispensing device and its dispensing method, and a sample analyzer and its dispensing method, to solve the technical problem in the prior art that when small volumes of liquid are drained, the droplets at the nozzle of the sampling needle are easily affected by the adhesion force of the liquid on the outer surface of the sampling needle, and thus wet and spread along the outer surface of the sampling needle, resulting in drainage failure.

[0006] To solve the above-mentioned technical problems, one technical solution adopted in this application is: providing a sampling device, which includes: a sampling needle, a first driving member, a second driving member, and a controller. The sampling needle is used to aspirate / displace liquid; the first driving member is connected to the sampling needle and is used to drive the sampling needle to move; the second driving member is connected to the sampling needle and is used to control the sampling needle to aspirate / displace liquid; the controller is connected to the first driving member and the second driving member, and is used to: control the first driving member to move the sampling needle to a liquid container, wherein the liquid in the liquid container covers the needle of the sampling needle. After the sampling needle enters the liquid container, the second driving component is controlled to draw liquid from the liquid container; the first driving component is controlled to move the needle tip away from the liquid surface of the liquid container; the second driving component is then controlled to draw a preset volume of gas from the sampling needle; the first driving component is controlled to move the sampling needle to an empty cup container; when the distance between the needle tip and the bottom wall of the empty cup container is within a preset range, the second driving component is controlled to discharge a droplet from the sampling needle. The droplet contacts the bottom wall and, under the pulling force of the bottom wall, detaches from the needle tip. The velocity of the droplet when it reaches the needle tip is greater than 0.

[0007] In some specific embodiments, the preset volume of the gas is greater than or equal to the volume corresponding to the liquid acceleration section.

[0008] In some specific embodiments, the controller is also used to: control the second drive unit so that when the sampling needle discharges the droplet, the velocity of the droplet when it reaches the nozzle of the sampling needle is less than 1.5 m / s.

[0009] In some specific embodiments, the controller is also used to: control the second drive unit so that when the sampling needle discharges the droplet, the velocity of the droplet when it reaches the tip of the sampling needle is greater than 0.3 m / s.

[0010] In some specific embodiments, the volume of the droplet expelled by the sampling needle each time is less than or equal to 10 microliters.

[0011] In some specific embodiments, the distance between the tip of the sampling needle and the bottom wall of the empty cup container is preset to a range of 1.7mm-0.5mm.

[0012] In some specific embodiments, the gas includes air.

[0013] To solve the above-mentioned technical problems, one technical solution adopted in this application is: providing a sampling method for a sampling device, based on any of the above embodiments of the sampling device, wherein the sampling method includes: controlling a first driving member to move a sampling needle to a liquid container, wherein after the liquid in the liquid container submerges the needle tip, controlling a second driving member to draw liquid from the liquid container; controlling the first driving member to move the needle tip away from the liquid surface of the liquid container, and then controlling the second driving member to draw a preset volume of gas from the sampling needle; controlling the first driving member to move the sampling needle to an empty cup container, wherein when the distance between the needle tip and the bottom wall of the empty cup container is within a preset range, controlling the second driving member to discharge a droplet from the sampling needle, wherein the droplet contacts the bottom wall and detaches from the needle tip under the pulling force of the bottom wall, wherein the velocity of the droplet when it reaches the needle tip is greater than 0.

[0014] To solve the above-mentioned technical problems, another technical solution adopted in this application is to provide a sample analyzer, which includes a sample addition device and a detection device according to any of the above embodiments. The sample addition device is used to add droplets to an empty cup container to form a sample to be tested in the empty cup container; the detection device is used to detect the sample to be tested.

[0015] To solve the above-mentioned technical problems, another technical solution adopted in this application is: to provide a sample addition method for a sample analyzer, based on any of the above embodiments of the sample analyzer, the sample addition method includes the sample addition method of any of the above embodiments.

[0016] The beneficial effects of this application are as follows: Unlike the prior art, the sampling device provided in this application includes a sampling needle, a first driving member, a second driving member, and a controller. The sampling needle is used to aspirate / discharge liquid. The first driving member is connected to the sampling needle and is used to drive the sampling needle to move. The second driving member is connected to the sampling needle and is used to control the aspiration / discharge of liquid by the sampling needle. The controller is used to: control the first driving member to move the sampling needle to a liquid container; after the liquid in the liquid container has submerged the needle tip, control the second driving member to aspirate liquid from the liquid container; control the first driving member to move the needle tip away from the liquid surface of the liquid container, and then control the second driving member to aspirate a preset volume of gas; control the first driving member to move the sampling needle to an empty cup container; when the distance between the needle tip and the bottom wall of the empty cup container is within a preset range, control the second driving member to discharge a droplet from the sampling needle; the droplet contacts the bottom wall and, under the pulling force of the bottom wall, detaches from the needle tip; wherein the velocity of the droplet when it reaches the needle tip is greater than 0. In this application, after the sampling needle collects the liquid, it draws in a section of gas to accelerate the liquid discharge. This increases the speed of the liquid at the needle tip, allowing the droplets to be discharged more quickly, reducing the influence of liquid adhesion on the outer wall of the sampling needle, eliminating the height limit of the liquid level above the needle tip during sampling, and improving sampling efficiency. Furthermore, during liquid discharge, the increased depth of the droplets relaxes the distance limit between the needle tip and the bottom wall of the empty cup, improving the efficiency of liquid discharge and enhancing the accuracy and repeatability of liquid discharge. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of a framework of an embodiment of a sample addition device provided in this application;

[0018] Figure 2 This is a schematic flowchart of an embodiment of a sample addition method of a sample addition device provided in this application;

[0019] Figure 3 This is a schematic diagram of an embodiment of a sampling needle being inserted into a liquid container to aspirate liquid;

[0020] Figure 4 This is a schematic diagram of an embodiment of the sampling needle being lifted after it has absorbed all the liquid.

[0021] Figure 5 This is a schematic diagram of an embodiment where the sampling needle draws up a portion of gas after drawing up the liquid;

[0022] Figure 6 This is a schematic diagram of an embodiment of the sampling needle draining liquid in an empty cup container;

[0023] Figure 7This is a schematic diagram of an embodiment of the sampling needle being raised after the liquid has been drained from the empty cup container;

[0024] Figure 8 This is a schematic diagram of the framework of an embodiment of a sample analyzer provided in this application. Detailed Implementation

[0025] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, it should be noted that, for ease of description, only the parts relevant to this application are shown in the accompanying drawings, not the entire structure. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this application.

[0026] The terms "first," "second," etc., used in this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or apparatuses.

[0027] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0028] When using sample analyzers, it is common to add small samples, such as 5 microliters, using a sampling needle. For small sample addition, it is necessary to prevent droplets from adhering to the needle tip and being unable to drain. Specifically, some liquid adheres to the outer surface of the sampling needle during sampling. The deeper the needle penetrates, the larger the surface area of ​​liquid adhering to the needle. During drainage, the liquid adhering to the needle's outer surface creates adhesion resistance, and droplets at the needle tip easily spread along the wetted area of ​​the needle's outer surface, thus preventing the droplets from falling normally. This application provides a sampling device that can be used for both large and small sample addition. For small sample addition, this device is particularly effective, eliminating the limitation that the sample must reach above the needle tip during aspiration and relaxing the distance requirement between the needle tip and the bottom of the cup during drainage, thus improving drainage efficiency and accuracy. The structure and method of the sampling device provided in this application will be described in detail below.

[0029] Please see Figure 1 As shown, Figure 1 This is a schematic diagram of an embodiment of a sample application device provided in this application. Specifically, the sample application device 10 includes: a sampling needle 11, a first driving member 13 and a second driving member 14 connected to the sampling needle 11, and a controller 12 connected to the first driving member 13 and the second driving member 14.

[0030] The sampling needle 11 is used to draw liquid from a liquid container and discharge the drawn liquid into an empty cup container. The liquid container contains liquid, which can be a sample, such as a blood sample, urine sample, or saliva sample. In other embodiments, the liquid can also be a reagent, such as a hemolytic agent or staining agent. This application does not specifically limit the type of liquid drawn by the sampling needle 11.

[0031] The sampling needle 11, after drawing liquid, can be used for draining small volumes of liquid (e.g., less than 10 microliters). The sampling device 10 of this application has a good effect on draining small volume droplets, which can improve the efficiency and accuracy of draining. It can also be understood that the sampling device 10 of this application can also be used for draining large volumes of liquid (e.g., greater than 10 microliters).

[0032] The first driving member 13 is connected to the sampling needle 11 and is used to drive the sampling needle 11 to move. Specifically, the first driving member 13 can drive the sampling needle 11 to move in the up, down, left, and right directions. For example, the first driving member 13 can drive the sampling needle 11 to move downwards so that the sampling needle 11 is inserted into the liquid container. After the needle tip of the sampling needle 11 is submerged in the liquid surface, it begins to draw liquid. After the sampling needle 11 has finished sampling, the first driving member 13 lifts the sampling needle 11 so that the sampling needle 11 is away from the liquid surface. The first driving member 13 may include a power mechanism such as a motor or a cylinder. The structure of the first driving member 13 is within the scope of what those skilled in the art can understand and will not be described in detail here.

[0033] The second driving element 14 is connected to the sampling needle 11 and is used to control the sampling needle 11 to draw in / out liquid. Specifically, when the sampling needle 11 moves into the liquid container and the tip of the sampling needle 11 is submerged below the liquid surface, the second driving element 14 controls the sampling needle 11 to draw in liquid. After drawing in liquid, the sampling needle 11 moves into an empty cup container, and the second driving element 14 controls the sampling needle 11 to discharge droplets into the empty cup container. The second driving element 14 may include a syringe or a metering pump or other power structure. The structure of the second driving element 14 is within the scope of understanding for those skilled in the art and will not be described in detail here.

[0034] The controller 12 is connected to the first drive unit 13 and the second drive unit 14, and the controller 12 is used to control the first drive unit 13 and the second drive unit 14. The first drive unit 13 and the second drive unit 14 drive the sampling needle 11 based on the control commands of the controller 12, so that the sampling needle 11 can perform liquid aspiration and drainage operations. Specifically, the controller 12 can control the first drive unit 13 to move the sampling needle 11 up, down, left, and right, and the controller 12 can also control the second drive unit 14 to make the sampling needle 11 perform liquid aspiration or drainage operations.

[0035] In the sampling device 10 of this application, the sampling needle 11 is capable of discharging liquid in mid-air. The controller 12, through reasonable control of the first driving component 13 and the second driving component 14, ensures that the sampling needle 11 absorbs a certain amount of gas after absorbing the liquid. This method allows the droplets to reach the needle tip of the sampling needle 11 at a faster speed during discharging, resulting in a rapid droplet fall and less influence from the adhesion force of the liquid on the outer surface of the sampling needle 11. Therefore, the sampling device 10 of this application can eliminate the height limitation of the liquid exceeding the needle tip of the sampling needle 11 during sampling, remove the limitation on the needle insertion speed of the sampling needle 11, and improve sampling efficiency. Moreover, the sampling needle 11 can increase the depth of droplet penetration during discharging, thus relaxing the distance requirement between the needle tip of the sampling needle 11 and the bottom wall of the empty container during discharging. The sampling method of the sampling device 10 will be described in detail below.

[0036] Please see Figure 2 As shown, Figure 2 This is a schematic flowchart of an embodiment of a sampling method using a sampling device provided in this application. This sampling method is implemented based on the structure of the sampling device 10 in any of the above embodiments. In some specific embodiments, the controller 12 can implement the following sampling method:

[0037] S11: Control the first driving unit to move the sampling needle to the liquid container. After the liquid in the liquid container covers the needle tip, control the second driving unit to make the sampling needle draw liquid from the liquid container.

[0038] When the sampling device 10 needs to add a sample, please refer to [reference needed]. Figure 3 As shown, the controller 12 controls the first drive unit 13 to move the sampling needle 11, so that the sampling needle 11 moves to the upper side of the liquid container 15, and then drives the sampling needle 11 to move down, so that the needle tip 111 of the sampling needle 11 is submerged in the liquid surface of the liquid container 15, so that the sampling needle 11 can draw liquid 20 from the liquid container 15.

[0039] After the sampling needle 11's nozzle 111 is submerged in the liquid container 15, some liquid 20 adheres to the outer surface of the sampling needle 11. When draining small-volume (less than 20 microliters) droplets, the liquid 20 on the outer surface of the sampling needle 11 may adsorb the droplets draining from the nozzle 111, preventing them from draining. To solve this problem, the prior art requires controlling the height of the sampling needle 11 submerged in the liquid container 15 during sampling, thereby reducing the amount of liquid on the outer surface of the sampling needle 11. Generally, the prior art limits the height of the liquid 20 above the nozzle 111 to less than 1 mm during sampling.

[0040] In this application, the sampling device 10 can eliminate the influence of the liquid 20 on the outer surface of the sampling needle 11 on the drainage. Therefore, the height of the liquid 20 above the needle opening 111 of the sampling needle 11 during sampling is not limited and can be greater than 1 mm. As a result, the sampling needle 11 can be inserted at high speed to find the liquid surface, so as to speed up the instrument and improve the sampling efficiency.

[0041] S12: Control the first driving component to make the sampling needle tip leave the liquid surface of the liquid container, and then control the second driving component to make the sampling needle draw up a preset volume of gas.

[0042] After the sampling needle 11 has drawn liquid from the liquid container 15, as follows: Figure 4 As shown, the controller 12 controls the first drive unit 13, which drives the sampling needle 11 away from the liquid surface of the liquid container 15, so that the sampling needle 11 is lifted away from the liquid surface.

[0043] like Figure 5As shown, the controller 12 then controls the sampling needle 11 to draw in a preset volume of gas, forming a gas column at the end of the sampling needle 11 as an acceleration section when the liquid 20 in the sampling needle 11 is discharged. The preset volume of gas is greater than or equal to the volume corresponding to the acceleration section when the liquid 20 is discharged from the sampling needle 11, so that the speed of the liquid 20 when it reaches the nozzle 111 of the sampling needle 11 reaches a predetermined value. During the process of the second driving member 14 driving the liquid and gas in the sampling needle 11 towards the nozzle of the sampling needle 11, the liquid and gas in the sampling needle 11 are in a stationary state before the second driving member 14 starts driving. This can be understood as the discharge speed of the liquid and gas in the sampling needle 11 being 0 at this time. Regardless of whether the second driving member 14 provides stable or variable power to the liquid and gas in the sampling needle 11, after the second driving member 14 starts driving, the discharge speed of the liquid and gas in the sampling needle 11 begins to increase from 0. During this process, the gas discharged from the sampling needle 11 with a speed gradually increasing from 0 is the acceleration section.

[0044] Optionally, the sampling needle 11 can draw in a further amount of air. In other embodiments, the sampling needle 11 can also draw in a further amount of inert gas such as nitrogen or carbon dioxide via the second drive member 14, thereby avoiding chemical reaction with the liquid 20 inside the sampling needle 11. The type of gas drawn in by the sampling needle 11 can be selected and set according to the chemical properties of the liquid 20.

[0045] The gas section at the end of the sampling needle 11 is used to accelerate the liquid 20 when the sampling needle 11 discharges liquid, so as to ensure that the droplets leave the needle opening 111 at a large and stable speed, thus ensuring the accuracy and reliability of the discharge.

[0046] Specifically, during drainage, the increase in velocity of the liquid 20 within the sampling needle 11 is a gradual process. During drainage, the gas in the acceleration section is gradually accelerated, resulting in a higher velocity when the liquid droplet reaches the needle opening 111. In other words, the discharged droplet has a higher velocity when leaving the needle opening 111. Compared to methods without an additional gas section, where the droplet velocity at the needle opening 111 starts from 0 and gradually increases, this application, by adding a gas section, enables the droplet to exit the needle opening 111 at a higher velocity.

[0047] S13: Control the first driving component to move the sampling needle to the empty cup container. When the distance between the sampling needle orifice and the bottom wall of the empty cup container is within a preset range, control the second driving component to discharge the droplet from the sampling needle. The droplet contacts the bottom wall and is pulled away from the sampling needle orifice by the bottom wall. The speed of the droplet when it reaches the sampling needle orifice is greater than 0.

[0048] After the sampling needle 11 draws in a section of air at its tip, as... Figure 6As shown, the controller 12 controls the first drive unit 13 to move the sampling needle 11 into the empty cup container 30 for air drainage.

[0049] When the distance between the nozzle 111 of the sampling needle 11 and the bottom wall of the empty cup container 30 is within a preset range, the second driving component 14 is controlled to cause the sampling needle 11 to discharge the droplet 40. The droplet 40 moves in the air column section of the sampling needle 11, and its speed begins to increase. When the droplet 40 reaches the nozzle 111 of the sampling needle 11, its speed is relatively high. Combined with the gravity of the droplet 40, the droplet 40 falls to the bottom wall of the empty cup container 30. After contacting the bottom wall of the empty cup container 30, the droplet 40 detaches from the nozzle 111 of the sampling needle 11 under the pulling force of the bottom wall of the empty cup container 30. Finally, as... Figure 7 As shown, the sampling needle 11 is lifted under the drive of the second driving member 14 to complete the current drainage. The drainage method of the sampling device 10 in this application is as follows: the liquid is not pulled off, but the liquid droplet 40 is adsorbed by the bottom wall of the empty cup container 30 to make the droplet detach from the sampling needle 11. With this drainage method, the detachment of the droplet 40 from the needle port 111 of the sampling needle 11 is easy to control and the sampling repeatability is good.

[0050] In the above embodiment, the droplet 40 has a relatively high speed when it just starts to detach from the nozzle of the sampling needle 11. Combined with the gravity of the droplet 40 itself, the droplet 40 dripping from the nozzle 111 of the sampling needle 11 can fall quickly. Then, relying on the adsorption force of the bottom wall of the empty cup container 30, it can better overcome the discharge resistance of the droplet 40 (the adhesion force of the liquid 20 on the outer surface of the sampling needle 11) and pull the droplet 40 into the empty cup container 30 to complete the liquid addition.

[0051] Furthermore, the velocity of the droplet 40 when it reaches the nozzle 111 of the sampling needle 11 is less than 1.5 m / s to avoid the droplet 40 breaking due to excessive velocity, which would affect the repeatability and reliability of the drainage. Preferably, the velocity of the droplet 40 when it reaches the nozzle 111 of the sampling needle 11 is less than 1 m / s. In this way, during the drainage process, the droplet can be drained quickly while preventing it from breaking, thus balancing drainage efficiency and drainage effect.

[0052] When the sampling needle 11 drains liquid, the velocity of the droplet 40 reaching the nozzle 111 of the sampling needle 11 is greater than 0.3 m / s. This ensures that the droplet 40 leaves the nozzle 111 at a relatively high velocity, thus preventing the droplet 40 from being affected by the adsorption of liquid 20 on the outer surface of the sampling needle 11. The depth to which the droplet 40 penetrates the bottom wall of the empty cup container 30 is increased, thereby widening the distance between the nozzle 111 of the sampling needle 11 and the bottom wall of the empty cup container 30 during drainage. This method improves both the drainage efficiency and accuracy.

[0053] Taking a sample dispensing droplet 40 with a volume of less than 10 microliters per dispensing cycle as an example, the distance between the tip 111 of the sampling needle 11 and the bottom wall of the empty cup container 30 can be within the range of 1.7-0.5 mm to achieve accurate dispensing. In contrast, during the non-gas-absorbing section, the distance between the tip 111 of the sampling needle 11 and the bottom wall of the empty cup container 30 needs to be within the range of 0.5-1 mm. Therefore, the sampling device 10 of this application significantly relaxes the distance requirement between the tip 111 of the sampling needle 11 and the bottom wall of the empty cup container 30.

[0054] The high-speed insertion of the sampling device 10 can easily result in the distance between the sampling needle 111 and the bottom wall of the empty cup container 30 exceeding 1 mm after insertion. However, the proposed solution relaxes the limitation on the insertion distance of the sampling needle 11. Therefore, the proposed solution can meet the requirements of instrument speed increase while ensuring the repeatability and accuracy of sample distribution.

[0055] The inventors of this application have conducted extensive experiments and found that the sample dispensing device 10 of any of the above embodiments is effective when applied to the dispensing of droplets 40 of no more than 10 microliters each time, and can significantly improve the repeatability and accuracy of sample dispensing.

[0056] In summary, the sampling device 10 provided in the above embodiments can perform aerial sampling, the droplet 40 is easily controlled to detach from the needle orifice 111, and the sampling repeatability is good. After the sampling needle 11 aspirates liquid, it aspirates an additional section of gas to act as an acceleration section when the droplet 40 is discharged. In this way, the droplet 40 reaches the needle orifice 111 of the sampling needle 11 at a relatively high speed. Combined with the gravity of the droplet 40, the droplet 40 falls rapidly, eliminating the influence of the liquid 20 on the outer surface of the sampling needle 11 on the droplet 40, and eliminating the limitation that the liquid level in the liquid container 15 does not exceed the height of the needle orifice 111 of the sampling needle 11 during liquid aspiration. This facilitates the rapid insertion of the sampling needle 11 during liquid aspiration and improves sampling efficiency.

[0057] The droplet 40 at the needle tip 111 of the sampling needle 11 falls rapidly, increasing the depth of the droplet 40, thereby widening the distance between the needle tip 111 and the bottom wall of the empty cup container 30 when the sampling needle 11 drains the liquid, which also helps to improve the drainage speed and the reliability of drainage.

[0058] This application also provides a sample analyzer; please refer to [link / reference]. Figure 8 As shown, Figure 8 This is a schematic diagram of a sample analyzer embodiment provided in this application. Specifically, the sample analyzer 80 includes a sample addition device 10 and a detection device 81. The sample addition device 10 is used to add droplets into an empty cup container in mid-air to form a sample to be tested in the empty cup container; the detection device 81 is used to detect the sample to be tested.

[0059] The sample application device 10 is any of the sample application devices 10 described above. For the structure of the sample application device 10, please refer to the description of the above embodiments, which will not be repeated here.

[0060] The sample analyzer 80 can be a blood sample analyzer or other liquid sample analyzer.

[0061] This application also provides a sample addition method for a sample analyzer. Based on the sample analyzer 80 of any of the above embodiments, the sample addition method includes the sample addition method of the sample addition device of any of the above embodiments. For the sample addition method of the sample addition device, please refer to the description of any of the above embodiments, which will not be repeated here.

[0062] The above description is merely an embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A sample dispensing device, characterized in that, The sampling device includes: Sampling needle, used for aspirating / draining liquid; A first driving element is connected to the sampling needle, and the first driving element is used to drive the sampling needle to move. The second driving component is connected to the sampling needle, and the second driving component is used to control the sampling needle to draw / expel liquid; A controller, connecting the first drive unit and the second drive unit, is used for: Control the first driving component to move the sampling needle to the liquid container. After the liquid in the liquid container covers the needle tip of the sampling needle, control the second driving component to make the sampling needle draw liquid from the liquid container. Control the first driving component to make the needle tip of the sampling needle leave the liquid surface of the liquid container, and then control the second driving component to make the sampling needle draw up a preset volume of gas; The first driving component is controlled to move the sampling needle to an empty cup container. When the distance between the needle tip of the sampling needle and the bottom wall of the empty cup container is within a preset range, the second driving component is controlled to discharge a droplet from the sampling needle. The droplet contacts the bottom wall and is pulled away from the needle tip of the sampling needle under the pull of the bottom wall. The velocity of the droplet when it reaches the needle tip of the sampling needle is greater than 0.

2. The sample addition device according to claim 1, characterized in that, The preset volume of the gas is greater than or equal to the volume of the liquid during the acceleration phase when it is discharged from the sampling needle.

3. The sample addition device according to claim 2, characterized in that, The controller is further configured to: control the second driving member so that when the sampling needle discharges the droplet, the velocity of the droplet reaching the needle opening of the sampling needle is less than 1.5 m / s.

4. The sample addition device according to claim 2, characterized in that, The controller is further configured to: control the second driving member so that when the sampling needle discharges the droplet, the velocity of the droplet when it reaches the needle opening of the sampling needle is greater than 0.3 m / s.

5. The sample dispensing device according to any one of claims 1-4, characterized in that, The volume of the droplet ejected by the sampling needle each time is less than or equal to 10 microliters.

6. The sample addition device according to claim 5, characterized in that, The preset range of the distance between the sampling needle tip and the bottom wall of the empty cup container is 1.7mm-0.5mm.

7. The sample addition device according to claim 1, characterized in that, The gas includes air.

8. A method for adding samples using a sample adding device, characterized in that, Based on the sampling device according to any one of claims 1-7, wherein the sampling method includes: The first driving unit is controlled to move the sampling needle to the liquid container. After the liquid in the liquid container covers the needle tip of the sampling needle, the second driving unit is controlled to make the sampling needle draw liquid from the liquid container. Control the first driving component to make the needle tip of the sampling needle leave the liquid surface of the liquid container, and then control the second driving component to make the sampling needle draw up a preset volume of gas; The first driving component is controlled to move the sampling needle to an empty cup container. When the distance between the needle tip of the sampling needle and the bottom wall of the empty cup container is within a preset range, the second driving component is controlled to discharge a droplet from the sampling needle. The droplet contacts the bottom wall and is pulled away from the needle tip of the sampling needle under the pull of the bottom wall. The velocity of the droplet when it reaches the needle tip of the sampling needle is greater than 0.

9. A sample analyzer, characterized in that, The sample analyzer includes: The sample dispensing device according to any one of claims 1-7 is used to add droplets to an empty cup container to form a sample to be tested in the empty cup container; A detection device for detecting the sample to be tested.

10. A sample addition method for a sample analyzer, characterized in that, Based on the sample analyzer of claim 9, the sample addition method includes the sample addition method of claim 8.

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