Horizontal screw centrifuge for yeast separation of nucleic acid

Abstract

The utility model provides a horizontal spiral centrifuge for separating nucleic acid from yeast. The horizontal spiral centrifuge comprises an outer shell, a rotary drum and a spiral which rotate coaxially are arranged in the outer shell, a liquid discharging cavity and a residue discharging cavity are formed in the two sides of the outer shell, a residue discharging head and a liquid discharging pump wheel are arranged at the two ends of the rotary drum, the residue discharging head is arranged in the residue discharging cavity to rotate, and the liquid discharging pump wheel is arranged in the liquid discharging cavity to rotate; a slag storage cylinder is fixedly arranged at a slag discharging opening in the bottom of the slag discharging cavity, and a cover plate opened through pressure induction is arranged at the bottom of the slag storage cylinder. By introducing an advanced automatic control system, an optimized mechanical structure and a high-efficiency cleaning system, the operation efficiency and safety of the equipment are improved, long-term stable operation is ensured, and the equipment is particularly suitable for the field of biotechnology application with high requirements for hygienic standards and operation safety. According to the design, the user experience is remarkably improved, and a reliable, efficient and easy-to-maintain solution is provided for the user.

Description

Technical Field

[0001] This utility model relates to the field of centrifuges, specifically to a horizontal screw centrifuge for separating nucleic acids from yeast. Background Technology

[0002] Existing horizontal decanter centrifuges, when used for separating nucleic acids from yeast, generate foam three times the volume of the clear liquid when the clear liquid is thrown into the clear liquid chamber. The large amount of foam requires storage space, which limits the efficiency of separating nucleic acids from yeast. There are also dead corners in the clear liquid chamber during cleaning, which can lead to microbial contamination. Furthermore, the separated particulate nucleic acids are scattered in all directions when thrown out of the slag outlet, making collection inconvenient. Utility Model Content

[0003] The main objective of this invention is to provide a horizontal screw centrifuge for separating nucleic acids from yeast, thereby solving the problems mentioned in the background art.

[0004] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows: it includes an outer shell, a rotating drum and a spiral that rotate coaxially inside the outer shell, a liquid discharge chamber and a slag discharge chamber on both sides of the outer shell, a slag discharge head and a liquid discharge pump wheel at both ends of the rotating drum, the slag discharge head rotating in the slag discharge chamber, and the liquid discharge pump wheel rotating in the liquid discharge chamber.

[0005] A slag storage cylinder is fixed at the slag discharge port at the bottom of the slag discharge chamber, and a cover plate that is opened by pressure sensing is provided at the bottom of the slag storage cylinder.

[0006] Preferably, bearing seats are provided on both sides of the outer casing, and the drum and the spiral abut against the bearing seats and rotate.

[0007] Preferably, a cleaning pipe is fixed at the top of the drainage chamber, and multiple nozzles are fixed on the cleaning pipe. The nozzles face the drainage pump wheel, and the cleaning pipe is connected to the cleaning liquid tank through the pump.

[0008] Preferably, one side of the drain pump wheel is connected to the drum through multiple overflow ports, and multiple arc-shaped plates are provided in the circumferential direction of the drain pump wheel, with the overflow ports located inside the arc-shaped plates.

[0009] Preferably, one side of the cover plate is hinged to the slag storage cylinder by a pin, and a connecting seat is fixed on one side of the slag storage cylinder. A hinged pushing mechanism is provided between the connecting seat and the cover plate. A pressure sensor is provided on the control pipeline of the pushing mechanism to detect the pressure during pushing.

[0010] Preferably, the pushing mechanism is a hydraulic cylinder, with the cylinder barrel end hinged to the connecting seat, the piston rod end hinged to the middle of the cover plate, and the pressure sensor installed in the oil circuit of the pushing oil port.

[0011] This invention provides a horizontal spiral centrifuge for isolating nucleic acids from yeast, with the following advantages:

[0012] 1. By pumping cleaning fluid from the nozzle, the drain pump impeller and drain chamber are efficiently cleaned, reducing manual intervention and improving work efficiency. A hydraulic cylinder combined with a pressure sensor automates the opening and closing of the slag storage cylinder cover, ensuring that solid waste is discharged at the appropriate time and simplifying the operation process.

[0013] 2. Pressure sensors monitor the hydraulic system pressure in real time to prevent overpressure and enhance equipment safety. When abnormally high pressure is detected, the system can automatically stop operation to avoid equipment damage or safety hazards. Precise control of the cover's opening and closing via the hydraulic system protects mechanical components from impact forces, extending the equipment's service life.

[0014] 3. The overflow port and arc-shaped plate design ensure that the separated liquid flows gently and efficiently into the drain pump impeller and is quickly discharged from the drain chamber, reducing turbulence and foam formation and improving cleaning efficiency. The automatic cleaning function helps maintain the cleanliness of the equipment's interior, making it particularly suitable for applications requiring high hygiene standards, such as yeast nucleic acid separation in biopharmaceutical production.

[0015] 4. The combined design of the overflow port and the arc-shaped plate makes full use of limited space, achieving efficient liquid management and making the entire device more compact, saving installation space. Since most mechanical actions are completed by the hydraulic system, wear on mechanical parts is reduced, the need for daily maintenance is lowered, and cleaning and inspection are also easier.

[0016] 6. The optimized liquid diversion and guiding design accelerates the drainage speed and shortens the time of each processing cycle, thereby improving overall operating efficiency. The automated slag removal mechanism reduces downtime and ensures continuous and efficient operation. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments:

[0018] Figure 1 This is a front sectional view of the overall structure of this utility model;

[0019] Figure 2 This is a utility model Figure 1 Sectional view of AA;

[0020] Figure 3 This is a side view of the connecting shaft of the discharge pump wheel of this utility model;

[0021] Figure 4 This is a schematic diagram of the cover plate connection structure of this utility model;

[0022] In the diagram: 1. Rotary drum; 2. Spiral; 3. Outer shell; 4. Slag discharge head; 5. Bearing seat; 6. Drainage chamber; 7. Cleaning pipe; 8. Slag discharge chamber; 9. Drainage pump wheel; 901. Overflow port; 902. Arc plate; 10. Drainage port; 11. Slag discharge port; 12. Slag storage cylinder; 13. Cover plate; 14. Connecting seat; 15. Pushing mechanism. Detailed Implementation

[0023] like Figures 1-4 As shown, a horizontal screw centrifuge for isolating nucleic acids from yeast includes an outer shell 3, inside which a coaxially rotating drum 1 and a spiral 2 are provided. On both sides of the outer shell 3, there are a liquid discharge chamber 6 and a slag discharge chamber 8. At both ends of the drum 1, there are slag discharge heads 4 and liquid discharge pump wheels 9. The slag discharge heads 4 rotate in the slag discharge chamber 8, and the liquid discharge pump wheels 9 rotate in the liquid discharge chamber 6.

[0024] A slag storage cylinder 12 is fixedly installed at the slag discharge port 11 at the bottom of the slag discharge chamber 8, and a cover plate 13 is provided at the bottom of the slag storage cylinder 12 that is opened by pressure sensing.

[0025] The separated liquid is discharged from the discharge pump wheel 9 into the discharge chamber 6, and the separated solid enters the discharge chamber 8 through the slag discharge head 4 and falls into the slag storage cylinder 12 from the slag discharge port 11 at the bottom. Through pressure sensing monitoring, when there is too much solid in the slag storage cylinder 12 and the pressure is too high, the control cover plate 13 is opened to discharge the material.

[0026] The outer casing 3 is the external frame of the entire centrifuge, housing all internal components and providing structural support. The drum 1 and the spiral 2 are coaxially mounted and rotate together under motor drive. The drum provides centrifugal force, while the spiral rotates inside the drum to push the solid material towards one end, thus achieving solid-liquid separation.

[0027] The two chambers, liquid discharge chamber 6 and slag discharge chamber 8, are located on both sides of the outer shell and are used to collect the separated liquid and solid waste. The liquid is discharged into the liquid discharge chamber through the liquid discharge pump wheel 9, while the solid is guided into the slag discharge chamber by the slag discharge head 4.

[0028] The slag discharge head 4 and the liquid discharge pump wheel 9 are located at opposite ends of the drum, helping to control the flow direction of the separated materials. The slag discharge head pushes the solids into the slag discharge chamber, while the liquid discharge pump wheel assists in the discharge of the liquid.

[0029] The slag storage cylinder 12 is located at the slag discharge port at the bottom of the slag discharge chamber and is used to temporarily store the solid material separated from the drum. When the pressure inside the slag storage cylinder reaches a certain threshold, the sensor will trigger the cover to open, allowing excess solids to be discharged from the slag discharge port.

[0030] Preferably, the outer casing 3 is provided with bearing seats 5 on both sides, and the drum 1 and the spiral 2 rotate against the bearing seats 5.

[0031] It also includes a drive unit for driving the drum 1 and the screw 2 to rotate.

[0032] Bearing housings 5 ​​are located on both sides of the housing and contain bearings to support the drum and auger, allowing them to rotate smoothly coaxially. Proper lubrication and maintenance are essential for maintaining the effective operation of the bearing housings.

[0033] The drive unit is mounted outside the housing and connected to the drum and auger via a transmission mechanism. When the motor starts, it transmits power to the drum and auger, causing them to rotate. For applications requiring fine speed adjustment, the drive unit may include a speed change mechanism to adjust the speed as needed.

[0034] Preferably, a cleaning pipe 7 is fixedly installed at the top of the drainage chamber 6, and multiple nozzles are fixedly installed on the cleaning pipe 7. The nozzles are directly facing the drainage pump wheel 9, and the cleaning pipe 7 is connected to the cleaning liquid tank through the pump.

[0035] After the separation operation is completed, during the cleaning process, the cleaning solution in the cleaning solution tank is pumped out from the nozzle on the cleaning pipe 7 to clean the drain pump wheel 9 and the drain chamber 6.

[0036] The cleaning pipe 7 is installed at the top of the drain chamber 6 and is responsible for delivering the cleaning fluid to each nozzle. Multiple nozzles are fixed to the cleaning pipe, and their positions and angles are precisely set to ensure that the sprayed cleaning fluid can directly cover the drain pump wheel 9 and the internal surface of the drain chamber 6. These nozzles are designed to provide sufficient pressure and coverage to effectively remove residues.

[0037] The pump is used to draw cleaning fluid from the tank into the cleaning pipe. This pump needs sufficient head to ensure the cleaning fluid is sprayed out of the nozzle at the appropriate pressure. The cleaning fluid tank stores a specially formulated cleaning fluid, which may contain disinfectants or other chemicals to ensure thorough cleaning and compliance with biosafety requirements.

[0038] After the separation operation is completed, the operator can start the cleaning procedure. The cleaning solution is drawn from the water tank and delivered to each nozzle through cleaning pipes via a pump. The high-pressure cleaning solution sprayed from the nozzles directly impacts the inner wall of the drain pump impeller 9 and the drain chamber 6, removing any residual yeast or nucleic acid material and preventing cross-contamination. The waste liquid after cleaning can be discharged through the drain port at the bottom of the drain chamber into a dedicated waste liquid treatment system.

[0039] Preferably, one side of the drain pump wheel 9 is connected to the drum 1 through multiple overflow ports 901, and multiple arc-shaped plates 902 are provided in the circumferential direction of the drain pump wheel 9, with the overflow ports 901 located inside the arc-shaped plates 902.

[0040] The liquid separated inside the drum 1 enters the drain pump wheel 9 from the overflow port 901, and is diverted and guided by the arc plate 902 to avoid the liquid directly impacting the drain chamber 6. At the same time, it can also accelerate the liquid in the drain chamber 6 to be discharged from the drain port 10.

[0041] Overflow ports 901 are located on one side of the discharge pump impeller and are connected to the inside of the drum. These overflow ports allow the separated liquid to flow smoothly from the inside of the drum into the discharge pump impeller. They are evenly distributed, ensuring the stability and uniformity of the liquid inflow.

[0042] The arc-shaped plate 902 is positioned around the overflow port along the circumference of the discharge pump impeller. The main function of the arc-shaped plate is to divert and guide the liquid flowing in from the overflow port, reducing the possibility of the liquid directly impacting the inner wall of the discharge chamber, while also helping the liquid to be discharged from the discharge port more quickly.

[0043] When the centrifuge is running, the liquid and solid matter separate under centrifugal force. The liquid moves towards the center of the drum through the inner wall and enters the discharge pump impeller 9 through the overflow port 901. The liquid entering the discharge pump impeller is divided into multiple small-flow streams by the arc-shaped plate 902. The design of the arc-shaped plate helps guide the liquid flow along a specific path, avoiding strong impact on the inner wall of the discharge chamber 6 and reducing the risk of turbulence and foam formation. Due to the presence of the arc-shaped plate, the liquid can flow more orderly in the discharge chamber, which not only improves the discharge efficiency but also reduces the residence time of the liquid in the discharge chamber, promoting the rapid discharge of the liquid from the discharge port 10.

[0044] Preferably, one side of the cover plate 13 is hinged to the slag storage cylinder 12 by a pin, and a connecting seat 14 is fixedly provided on one side of the slag storage cylinder 12. A hinged pushing mechanism 15 is provided between the connecting seat 14 and the cover plate 13. A pressure sensor is provided on the control pipeline of the pushing mechanism 15 to detect the pressure during pushing.

[0045] The jacking mechanism 15 is a hydraulic cylinder. The cylinder end is hinged to the connecting seat 14, and the piston rod end is hinged to the middle of the cover plate 13. The pressure sensor is installed in the oil circuit of the jacking oil port.

[0046] One side of the cover plate 13 is hinged to the slag storage cylinder 12 by a pin, allowing the cover plate to rotate between opening and closing. The connecting seat 14 is fixed to one side of the slag storage cylinder 12, providing a stable mounting point for the hydraulic cylinder.

[0047] A pressure sensor is installed in the hydraulic circuit of the hydraulic cylinder's jacking port to detect pressure changes in the hydraulic system in real time. This not only helps monitor the force exerted during the jacking process but also serves as part of a safety mechanism to prevent overpressure.

[0048] When solid matter accumulates to a certain level in the slag storage cylinder, the internal pressure increases, triggering the sensor. The control system then sends a command to the hydraulic system. Hydraulic oil enters the hydraulic cylinder through the oil port, pushing the piston rod to retract, thereby controlling the opening of the cover plate 13.

[0049] The above embodiments are merely preferred technical solutions of this utility model and should not be considered as limitations on this utility model. The protection scope of this utility model should be the technical solution described in the claims, including equivalent substitutions of the technical features described in the claims. That is, equivalent substitutions and improvements within this scope are also within the protection scope of this utility model.

Claims

1. A horizontal spiral centrifuge for isolating nucleic acids from yeast, characterized in that: Includes an outer shell (3), inside which is a coaxially rotating drum (1) and a spiral (2), and on both sides of the outer shell (3) are a liquid discharge chamber (6) and a slag discharge chamber (8), and at both ends of the drum (1) are a slag discharge head (4) and a liquid discharge pump wheel (9), the slag discharge head (4) is located in the slag discharge chamber (8) and rotates, and the liquid discharge pump wheel (9) is located in the liquid discharge chamber (6) and rotates; A slag storage cylinder (12) is fixed at the slag discharge port (11) at the bottom of the slag discharge chamber (8), and a cover plate (13) is provided at the bottom of the slag storage cylinder (12) that is opened by pressure sensing.

2. The horizontal spiral centrifuge for isolating nucleic acids from yeast according to claim 1, characterized in that: The outer shell (3) has bearing seats (5) on both sides, and the drum (1) and the screw (2) rotate against the bearing seats (5).

3. The horizontal spiral centrifuge for isolating nucleic acids from yeast according to claim 1, characterized in that: A cleaning pipe (7) is fixedly installed at the top of the drain chamber (6). Multiple nozzles are fixedly installed on the cleaning pipe (7). The nozzles face the drain pump wheel (9). The cleaning pipe (7) is connected to the cleaning liquid tank through the pump.

4. The horizontal spiral centrifuge for isolating nucleic acids from yeast according to claim 1, characterized in that: The side of the discharge pump wheel (9) is connected to the drum (1) through multiple overflow ports (901). Multiple arc plates (902) are provided in the circumferential direction of the discharge pump wheel (9), and the overflow ports (901) are located inside the arc plates (902).

5. A horizontal spiral centrifuge for isolating nucleic acids from yeast according to claim 1, characterized in that: One side of the cover plate (13) is hinged to the slag storage cylinder (12) by a pin. A connecting seat (14) is fixed on one side of the slag storage cylinder (12). A hinged jacking mechanism (15) is provided between the connecting seat (14) and the cover plate (13). A pressure sensor is provided on the control pipeline of the jacking mechanism (15) to detect the pressure during jacking.

6. A horizontal spiral centrifuge for isolating nucleic acids from yeast according to claim 5, characterized in that: The jacking mechanism (15) is a hydraulic cylinder. The cylinder end of the hydraulic cylinder is hinged to the connecting seat (14), and the piston rod end is hinged to the middle of the cover plate (13). The pressure sensor is installed in the oil circuit of the jacking oil port.

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