Reagent mixing container and detection instrument comprising same

By using elastic conduction components and centrifugal forces in the reagent mixing container, the automatic mixing of reagents is solved, and the problem of poor timeliness and repetition caused by manual operation in the prior art is improved, and the accuracy of the test and operation standardization are improved.

CN222111591UActive Publication Date: 2024-12-06BASO DIAGNOSTICS INC ZHUHAI
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Patent Information

Application Number
CN202420796345.1
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-17
Publication Date
2024-12-06
Estimated Expiration
2034-04-17

AI Technical Summary

Technical Problem

In the prior art, the reagent mixing operation process relies on manual dropping, resulting in poor timeliness and repetition, and lack of standardized operation procedures.

Method used

A reagent mixing container is designed, including independent working cavity and elastic conduction assembly, and the automatic mixing of reagents is achieved by centrifugal force. By setting independent cavity and elastic conduction assembly in the container body, the elastic conduction assembly is in Under the action of centrifugal force, the conduction between the cavity is achieved and the automatic mixing of reagents is achieved.

Benefits of technology

The reagent detection operation process is simplified, the accuracy and repeatability of the test are improved, the errors caused by operators are reduced, and the reagents are automatically mixed.

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Abstract

The utility model relates to the technical field of medical instruments, and discloses a reagent mixing container and a detection instrument comprising the same, the mixing container is matched with a centrifugal machine, the mixing container comprises a container body and an elastic conduction assembly, and the elastic conduction assembly is arranged along the axial direction of the container body. A working cavity, a first cavity and a second cavity are independently arranged in the container body, the first cavity and the second cavity are used for containing reagents, a first through hole is formed between the working cavity and the first cavity, and a second through hole is formed between the working cavity and the second cavity; and the elastic conduction assembly is installed in the working cavity, and the elastic conduction assembly extends from the bottom of the working cavity to the top of the working cavity and at least extends to completely block the first through hole or the second through hole. The functions of automatically adding reagents, uniformly mixing, centrifuging, removing supernate and shaking and re-suspending are realized by physical means, and compared with automatic equipment which is widely applied to biochemical detection, the standard operation process is improved, and meanwhile, the portability and cost advantages in the experiment process are reserved to the greatest extent.
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Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a reagent mixing container. Background Art

[0002] At present, in clinical transfusion compatibility testing, condensing amine medium reagents are widely used as auxiliary agents for the screening and detection of complete antibodies and incomplete antibodies and for cross-matching experiments and other blood bank work due to their convenience, speed and high sensitivity. Condensing amine reagents are mostly composed of three solutions: low-ion solution, condensing amine solution and suspension. The use process is to add reagents in sequence and mix the reaction system, let it stand, centrifuge, remove the supernatant and other operations according to the steps. In the prior art, most of the processes for mixing two or more reagents are manually added by staff, which has a certain degree of impact on the timeliness and repeatability of the test, and the effects vary significantly between different operators. There is a lack of standardized operating procedures. In existing operations, in addition to the use of complex automated equipment, there are few improvements to the reagent mixing container itself.

[0003] Therefore, how to provide a device that can automatically mix reagents to achieve standardization of operating procedures is an urgent problem that those skilled in the art need to solve. Utility Model Content

[0004] In view of this, the utility model provides a reagent mixing container to solve the problem that the timeliness and repeatability of the test are poor due to manual operation in the existing reagent mixing operation process.

[0005] In order to solve the above technical problems, the utility model adopts the following technical solutions:

[0006] A reagent mixing container, the mixing container is placed obliquely in the centrifuge, and the mixing container comprises:

[0007] A container body, wherein a mutually independent working cavity, a first cavity and a second cavity are provided inside the container body, and the working cavity, the first cavity and the second cavity are all extended along a first direction, the first direction is a direction from the closed end of the container body toward the open end of the container body, a first through hole is provided between the working cavity and the first cavity, and a second through hole is provided between the working cavity and the second cavity;

[0008] An elastic conductive component is installed in the working chamber, the elastic conductive component extends from the bottom to the top of the working chamber and at least extends to completely block one of the first through hole and the second through hole, the distance from the first through hole to the open end of the container body is greater than the distance from the second through hole to the open end of the container body; from the closed end of the working chamber toward the open end, the distance between the axis of the working chamber and the axis of the centrifuge gradually decreases.

[0009] Preferably, the elastic conductive component includes a first elastic member and a first working part; the first elastic member is arranged at the bottom of the working chamber, one end of the first working part abuts against the first elastic member, and the other end extends toward the opening end of the working chamber and at least extends to completely block one of the first through hole and the second through hole. Under the action of centrifugal force, the first elastic member is compressed to gradually increase the distance from the top of the elastic conductive component to the axis of the centrifuge, so that the first through hole is connected to the second through hole.

[0010] Preferably, the mixing container further comprises a first connecting component and a third cavity; the first connecting component is detachably arranged on the top of the container body, a third cavity is arranged inside the first connecting component, and the third cavity can be connected with the first cavity in a working state.

[0011] Preferably, a pressing piece is slidably arranged in the third cavity, and the end of the pressing piece close to the first cavity is enclosed with the inner wall of the third cavity to form a reagent cavity.

[0012] Preferably, the first connecting component includes a first accommodating cavity, which is formed by the inner wall of the first connecting component, the outer edge of the third cavity and the outer edge of the container body, the top of the third cavity extends away from the container body and at least extends to be flush with the outer edge of the top of the first accommodating cavity, and a water absorbing layer is arranged in the first accommodating cavity.

[0013] Preferably, the first connecting component further includes a second accommodating cavity, the second accommodating cavity is arranged at the top of the first accommodating cavity, and the second accommodating cavity is adapted to the top of the third cavity so that the third cavity is located in the first connecting component.

[0014] Preferably, the second connecting component is provided with a sealing portion at one end close to the container body, and the liquid outlet at the bottom of the third cavity abuts against the sealing portion.

[0015] In addition, the utility model also provides a detection instrument, comprising any of the reagent mixing containers described above.

[0016] The utility model provides a reagent mixing container, which has the following beneficial effects compared with the prior art:

[0017] The utility model effectively simplifies the operation process of reagent detection by improving the reagent mixing container itself, improves the accuracy and repeatability of the test, and reduces the operation error caused by the operator. Specifically, the utility model improves the container body, opens independent cavities in the container body to accommodate different reagents, and opens a working cavity in the container body to accommodate the elastic conductive component, and at the same time combines the centrifugal force generated by the centrifugal process, under the action of the centrifugal force, the elastic conductive component is displaced in the working cavity, and the distance from the top of the elastic conductive component to the centrifuge transmission shaft gradually increases, thereby making the first cavity and the second cavity mutually conductive, and realizing automatic mixing of different reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0019] Figure 1 It is a cross-sectional view of the overall structure of the reagent mixing container of the utility model;

[0020] Figure 2 It is a three-dimensional structural schematic diagram of the reagent mixing container of the utility model;

[0021] Figure 3 It is a schematic diagram of the structure of the reagent mixing container of the utility model except the container body;

[0022] Figure 4 It is a schematic diagram of the container body in the reagent mixing container of the utility model in a non-working state;

[0023] Figure 5 It is a schematic diagram of the container body in the reagent mixing container of the utility model in a working state;

[0024] Figure 6 It is a schematic diagram of the final state of the reagent mixing container of the utility model when in use.

[0025] In the figure:

[0026] 100-container body, 110-first cavity, 111-first through hole, 120-second cavity, 121-second through hole, 130-working cavity, 131-first elastic member, 132-first working part, 200-first connecting assembly, 210-first accommodating cavity, 220-water absorbing layer, 230-second accommodating cavity, 300-third cavity, 310-reagent cavity, 320-second elastic member, 330-second working part, 400-second connecting assembly, 500-sealing part. DETAILED DESCRIPTION

[0027] The following is a further detailed description of the specific implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0028] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0029] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0030] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0031] like Figure 1 As shown, on the one hand, the embodiment of the utility model provides a reagent mixing container, and the mixing container is placed obliquely in the centrifuge. It should be noted that the utility model only describes the structure of the mixing container, and the size of the mixing container can be designed into a variety of different models according to the size of the test tube rack in the centrifuge. In addition, the "top", "bottom", etc. involved below are all based on the reagent mixing container. Figure 1Description of the status shown.

[0032] like Figure 4-5 As shown, in some embodiments of the present invention, the mixing container includes a container body 100 and an elastic conductive component. When the container body 100 is tilted and placed in the centrifuge, the angle between the container body 100 and the horizontal plane is α, and α is less than 90°.

[0033] Among them, along the axial direction of the container body 100, a working cavity 130 and a first cavity 110 and a second cavity 120 for containing reagents are independently arranged inside the container body 100, and the working cavity 130, the first cavity 110 and the second cavity 120 are all extended along the first direction, and the first direction is the direction from the closed end of the container body 100 toward the open end of the container body 100; a first through hole 111 is opened between the working cavity 130 and the first cavity 110, and a second through hole 121 is opened between the working cavity 130 and the second cavity 120; the elastic conductive component is installed in the working cavity 130, and the elastic conductive component extends from the bottom to the top of the working cavity 130 and at least extends to completely block one of the first through hole 111 and the second through hole 121; from the closed end of the working cavity 130 toward the open end, the distance between the axis of the working cavity and the axis of the centrifuge gradually decreases.

[0034] Understandable, such as Figure 4 As shown, in the non-working state, the first through hole 111 and / or the second through hole 121 are blocked by the elastic conductive component, so that the reagent in the second cavity 120 cannot enter the first cavity 110; and as shown Figure 5 As shown, in the working state, the centrifuge applies centrifugal force to the elastic conductive component. At this time, the elastic conductive component moves from the top to the bottom of the working chamber 130 under the action of centrifugal force, and the distance from the top of the elastic conductive component to the centrifuge drive shaft gradually increases, that is, during the movement, the blocking part of the first through hole 111 and / or the second through hole 121 is released, and the first through hole 111 and the second through hole 121 are connected, thereby realizing the connection between the first cavity 110 and the second cavity 120, that is, under the action of centrifugal force, the reagent in the second cavity 120 enters the first cavity 110, and the two reagents in the two cavities are mixed.

[0035] Furthermore, the distance from the first through hole to the open end of the container body is greater than the distance from the second through hole to the open end of the container body. Under the dual effects of gravity and centrifugal force, the reagent in the second cavity can more easily enter the first cavity for mixing.

[0036] In some embodiments of the present invention, the elastic conductive component includes a first elastic member 131 and a first working portion 132; the first elastic member 131 is arranged at the bottom of the working cavity 130, one end of the first working portion 132 abuts against the first elastic member 131, and the other end extends toward the open end of the working cavity 130 and at least extends to completely block the first through hole 111 and / or the second through hole 121.

[0037] Optionally, the cross-sectional area of ​​the first working portion 132 is equal to the cross-sectional area of ​​the working chamber 130. Under the action of centrifugal force, the first elastic member 131 is compressed to move the elastic conductive component toward the bottom of the working chamber 130, and the distance from the top of the elastic conductive component to the axis of the centrifuge gradually increases, so that the first through hole 111 is connected with the second through hole 121, and then the reagent in the second chamber 120 passes through the second through hole 121, the working chamber 130, and the first through hole 111 in turn and then enters the first chamber 110, thereby realizing centrifugal mixing of the two reagents.

[0038] It can be understood that the first cavity 110 serves as the final mixing area for all reagents, there is only one first cavity 110 and its volume is relatively large, and the reagent in the second cavity 120 is added to the first cavity 110 as an additive. Since the type of additive may not be unique depending on the specific test, the second cavity 120 can be provided with multiple second cavities 120, and the multiple second cavities 120 are independent of each other and not connected.

[0039] In some embodiments of the present invention, Figure 1-3 As shown, the mixing container also includes a first connecting component 200 and a third cavity 300; the first connecting component 200 is detachably fixed to the top of the container body 100, and the third cavity 300 is arranged inside the first connecting component. In the working state, the third cavity 300 can be connected with the first cavity 110.

[0040] Specifically, the first connecting component 200 and the third cavity 300 can be two independent structures or an integrally formed structure. When the first connecting component 200 and the third cavity 300 are two independent structures, the first connecting component 200 is provided with a first limiting hole, and the third cavity 300 is installed on the first connecting component 200 through the first limiting hole.

[0041] It is understandable that when it comes to reagents that need to be added after centrifugation, the container body 100 can cooperate with the first connecting component 200 and the third cavity 300 to form a mixing container. After the reagents in the first cavity 110 and the second cavity 120 are centrifuged and mixed, the first connecting component 200 can be connected to the container body 100 to achieve the operation of dripping the reagent in the third cavity 300 into the first cavity 110.

[0042] In some embodiments of the present invention, a pressing piece is slidably arranged in the third cavity 300, and the end of the pressing piece close to the first cavity 110 is enclosed with the inner wall of the third cavity 300 to form a reagent cavity 310. Under external pressure, the pressing piece moves toward the reagent cavity 310 to allow the reagent in the reagent cavity 310 to enter the first cavity 110.

[0043] It can be understood that by setting a pressing piece on the top of the reagent chamber 310, on the one hand, a certain degree of sealing can be formed for the reagent in the reagent chamber 310, thereby preventing the reagent in the reagent chamber 310 from overflowing during transportation; on the other hand, in the working state, by pressing the pressing piece, the reagent in the reagent chamber 310 can be controllably immersed in the first cavity 110, thereby achieving quantitative control.

[0044] In some embodiments of the present invention, the pressing member includes a second elastic member 320 and a second working part 330; one end of the second elastic member 320 is close to the bottom of the third cavity 300 and forms a reagent cavity 310 with the inner wall of the third cavity 300, and the other end of the second elastic member 320 extends toward the top of the third cavity 300 to abut against the second working part 330.

[0045] It is understandable that in the working state, pressing the second working part 330 can drive the second elastic member 320 to move toward the bottom of the third cavity 300 , thereby causing the reagent in the reagent cavity 310 to drip into the first cavity 110 .

[0046] The second elastic member 320 may be a spring, and the combination of the second elastic member 320 and the second working part 330 may ensure the three-dimensional stability of the third cavity 300 during the actual assembly, production and transportation. Since the amount of each component reagent is required to be relatively accurate in most reaction systems, conventional pressure heads such as soft rubber drippers may cause reagents to overflow due to extrusion deformation during assembly or transportation, affecting the amount of reagents that ultimately participate in the reaction.

[0047] In some embodiments of the utility model, the first connecting component 200 includes a first accommodating cavity 210, which is formed by the inner wall of the first connecting component 200, the outer edge of the third cavity 300 and the outer edge of the container body 100. The top of the third cavity 300 extends away from the container body 100 and at least extends to be flush with the outer edge of the top of the first accommodating cavity 210. A water absorbing layer 220 is arranged in the first accommodating cavity 210.

[0048] It is understandable that, since it is usually necessary to perform a step of removing the supernatant after the reagent centrifugal mixing is completed, by providing a water-absorbing layer 220, the container body 100 can be poured in the step of removing the supernatant, so that the supernatant in the first cavity 110 is adsorbed in the water-absorbing layer 220, and the supernatant is removed. The operation is simple and convenient and it is safer and more hygienic. Preferably, the material of the water-absorbing layer 220 can be absorbent foam, and there is no special limitation on this. It only needs to have a material with water absorption properties. In addition, the purpose of limiting the top of the third cavity 300 to be flush with the outer edge of the top of the first accommodating cavity 210 or exceeding the outer edge of the top of the first accommodating cavity 210 is to prevent the supernatant from flowing back into the third cavity 300 during the pouring process, so as to ensure the accuracy of the detection process.

[0049] In some embodiments of the present invention, the first connecting component 200 also includes a second accommodating cavity 230, which is arranged on the top of the first accommodating cavity 210, and the second accommodating cavity 230 is adapted to the top of the third cavity 300 so that the third cavity 300 is located in the first connecting component 200.

[0050] Furthermore, the mixing container also includes a second connecting component 400, which is detachably arranged between the first connecting component 200 and the container body 100, and the second connecting component 400 is provided with a second limiting hole matching the first limiting hole, so that the third cavity 300 can be fixed after passing through the first limiting hole and the second limiting hole in sequence.

[0051] Furthermore, a sealing portion 500 is provided at one end of the second connecting assembly 400 close to the container body 100 , and the liquid outlet at the bottom of the third cavity 300 abuts against the sealing portion 500 .

[0052] It can be understood that the second connection component 400 can not only realize the connection between the first connection component 200 and the container body 100 to form a complete set of reagent detection devices, but also through the combination of the second connection component 400, the first accommodating chamber 210 and the second accommodating chamber 230, it can ensure that the third cavity 300 is not exposed during transportation, and avoid the situation where the third cavity 300 is damaged during transportation and causes the reagent to overflow. In addition, the sealing part 500 is in contact with the liquid outlet at the bottom of the third cavity 300, which can prevent the reagent in the third cavity 300 from entering the first cavity 110 in advance and affecting the reagent detection process.

[0053] On the other hand, an embodiment of the utility model further provides a detection instrument including any of the above-mentioned reagent mixing containers, such as a centrifuge. During use, the reagent mixing container can be placed in the centrifuge to detect the sample, and the detection method is simple and quick.

[0054] Next, the reagent mixing container is described in more detail by taking the use of the coagulation amine medium reagent as an example.

[0055] The reagent mixing container includes a container body 100, in which a first cavity 110, a second cavity 120 and a working cavity 130 are independently arranged, wherein the first cavity 110 and the second cavity 120 are respectively filled with a low-ion medium and a condensation amine solution, and an elastic conductive component is arranged in the working cavity 130, and the elastic conductive component includes a first elastic member 131 and a first working part 132, for example, it can be a spring and a piston. Under normal conditions, the piston of the first working part 132 blocks the first through hole 111 and / or the second through hole 121 through the action of the spring of the first elastic member 131. During operation, under the action of centrifugal force, the spring is deformed by force to move the piston downward to connect the first through hole 111 and the second through hole 121, and the condensation amine solution in the second cavity 120 immediately enters the first cavity 110 and joins the reaction system.

[0056] The reagent mixing container also includes a first connecting component 200, a second connecting component 400 and a third cavity 300. The third cavity 300 is a dropper structure, and a second working part 330, a second elastic member 320 and a reagent cavity 310 for containing a heavy suspension are sequentially arranged inside the third cavity 300 from top to bottom. Before the mixing container is used, the first connecting component 200 and the container body 100 are connected through the second connecting component 400. The second connecting component 400 can be a connecting sleeve. The first connecting component 200 acts as a cover of the third cavity 300, and a water-absorbing foam is arranged inside the first connecting component 200 as a water-absorbing layer 220. The liquid outlet of the third cavity 300 is abutted with a sealing rubber pad as a sealing part 500. When working, the liquid in the reagent cavity 310 is squeezed out by pressing the second working part 330, so as to achieve the purpose of dripping the reagent in the third cavity 300 into the first cavity 110.

[0057] The working principle of the reagent mixing container is as follows: when the container body 100 is placed in the centrifuge, the angle between the container body 100 and the horizontal plane is α, α < 90°, the centrifugal parameters commonly used in the polycondensation methodology are about 1000g, and the horizontal force of the container body 100 during the acceleration process is F 水平 In the working state, the axial downward pressure on the first elastic member 131 is approximately F 压 =F 水平 *cosα, the force and deformation of the first elastic member 131 are positively correlated F=Kx, where x is the deformation distance of the spring force, and K is the stiffness coefficient, which is determined by the material, number of turns, and area of ​​the spring. When the spring is pressed down to deform to meet the requirement that the downward movement distance of the first working portion 132 exceeds the height difference between the first through hole 111 and the second through hole 121 and the plane of the container body 100, the first through hole 111 and the second through hole 121 are connected, so that the reagent in the second cavity 120 is immersed in the first cavity 110 to participate in the reaction.

[0058] The procedure for using the reagent mixing container is as follows:

[0059] (1) Unscrew the second connecting component 400 and the first connecting component 200, tear off the sealing aluminum film on the top of the container body 100, add the sample to be tested into the first cavity 110, and put the container body 100 into the centrifuge for centrifugation; the container body 100 is placed at an angle such as Figure 3 As shown, from the open end to the closed end of the container body 100, the distance between the container body 100 and the centrifuge drive shaft increases, that is, the container body 100 is tilted toward the centrifuge drive shaft.

[0060] (2) After balancing, the centrifuge is run at a low speed for 60 seconds to allow the sample to be tested to be fully mixed and reacted with the low-ion medium in the first cavity 110. At the same time, the polycondensate solution in the second cavity 120 enters the first cavity 110 under the action of centrifugation. After the centrifugation is completed, the centrifuge is allowed to stand for 15 seconds;

[0061] (3) The centrifuge is centrifuged at the test speed for 10 seconds. Under the action of the reagent and the centrifugal force, the reactants will be deposited at the bottom of the first cavity 110. After the procedure is completed, the container body 100 is taken out;

[0062] (4) Figure 6 As shown, the first connecting assembly 200 is installed to the open end of the container body 100, and the container body 100 is tilted so that the supernatant is absorbed by the absorbent foam, thereby completing the function of removing the supernatant;

[0063] (5) Press the second working part 330 to make the resuspended liquid in the reagent chamber 310 drip into the first cavity 110, gently shake the container body 100, and read the result.

[0064] The utility model realizes the functions of automatic reagent addition, mixing, centrifugation, supernatant removal and shaking resuspension by physical means, and cooperates with a centrifuge to complete automatic sample addition in stages. Compared with the automated equipment widely used in biochemical tests, the utility model improves the standardized operation process while retaining the portability and cost advantage of the experimental process to the greatest extent.

[0065] The above is only a preferred embodiment of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and substitutions without departing from the technical principle of the present invention. These improvements and substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A reagent mixing container, characterized in that: The mixing container is placed obliquely in the centrifuge, and the mixing container comprises: A container body, wherein a mutually independent working cavity, a first cavity and a second cavity are provided inside the container body, and the working cavity, the first cavity and the second cavity are all extended along a first direction, the first direction is a direction from the closed end of the container body toward the open end of the container body, a first through hole is provided between the working cavity and the first cavity, and a second through hole is provided between the working cavity and the second cavity; An elastic conductive component is installed in the working chamber, the elastic conductive component extends from the bottom to the top of the working chamber and at least extends to completely block one of the first through hole and the second through hole; the distance from the first through hole to the open end of the container body is greater than the distance from the second through hole to the open end of the container body, and the distance between the axis of the working chamber and the axis of the centrifuge gradually decreases from the closed end of the working chamber toward the open end.

2. The reagent mixing container according to claim 1, characterized in that: The elastic conductive component includes a first elastic member and a first working portion; The first elastic member is arranged at the bottom of the working chamber, one end of the first working part abuts against the first elastic member, and the other end extends toward the opening end of the working chamber and at least extends to completely block one of the first through hole and the second through hole. Under the action of centrifugal force, the first elastic member is compressed to gradually increase the distance from the top of the elastic conductive component to the axis of the centrifuge, so that the first through hole is conductive with the second through hole.

3. The reagent mixing container according to claim 1, characterized in that: The mixing container also includes a first connecting assembly and a third cavity; The first connecting component is detachably arranged on the top of the container body, and a third cavity is arranged inside the first connecting component. In a working state, the third cavity can be communicated with the first cavity.

4. The reagent mixing container according to claim 3, characterized in that: A pressing piece is slidably arranged in the third cavity, and the end of the pressing piece close to the first cavity is enclosed with the inner wall of the third cavity to form a reagent cavity.

5. The reagent mixing container according to claim 3, characterized in that: The first connecting component includes a first accommodating cavity, which is formed by the inner wall of the first connecting component, the outer edge of the third cavity and the outer edge of the container body. The top of the third cavity extends away from the container body and at least extends to be flush with the outer edge of the top of the first accommodating cavity. A water absorbing layer is arranged in the first accommodating cavity.

6. The reagent mixing container according to claim 5, characterized in that: The first connection component also includes a second accommodating cavity, which is arranged at the top of the first accommodating cavity, and the second accommodating cavity is adapted to the top of the third cavity, so that the third cavity is located in the first connection component.

7. The reagent mixing container according to claim 3, characterized in that: The mixing container further comprises a second connecting component, which is detachably arranged between the first connecting component and the container body.

8. The reagent mixing container according to claim 7, characterized in that: The second connecting component is provided with a sealing portion at one end close to the container body, and the liquid outlet at the bottom of the third cavity abuts against the sealing portion.

9. A detection instrument, characterized in that: A reagent mixing container comprising the reagent mixing container according to any one of claims 1 to 8.