Vacuum closed pathogen nucleic acid detection device

Abstract

The utility model discloses a vacuum closed pathogen nucleic acid detection device which comprises a plurality of groups of reagent tubes for storing reaction liquid; a plurality of groups of storage boxes are arranged, and a plurality of vertically-through storage cavities are formed in the storage boxes and are used for independently storing reagent tubes; the refrigerating box is used for storing a plurality of groups of storage boxes and taking and placing the storage boxes in a drawing and pulling mode; the utility model relates to the technical field of nucleic acid detection. According to the vacuum closed pathogen nucleic acid detection device, the refrigeration box is used for storing a detection sample, the effects of refrigeration and heat preservation can be achieved, the device is suitable for collecting nucleic acid samples outdoors for a long time, the drawer drawing type storage box is used for storing reagent tubes, a group of nucleic acid samples can be pushed into one grid after being put into the storage box, and the nucleic acid samples can be kept to be refrigerated in time; meanwhile, the reagent tube adopts an up-and-down isolated structure, the swab is inserted into the lower layer of the valve, stirred and then drawn out, and the lower layer reaction liquid can be kept in a vacuum closed state and is not in contact with air, so that the nucleic acid sample can be prevented from deteriorating.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection, in particular to a vacuum-sealed pathogen nucleic acid detection device. Background Art

[0002] Nucleic acid testing is to find out whether the patient's respiratory specimens, blood or feces contain nucleic acids of foreign invading viruses to determine whether the patient is infected with the virus. After obtaining the patient's sample, the test must be carried out as soon as possible. If the sample cannot be tested immediately and needs to be transported, it should be packaged at low temperature in accordance with the requirements of the instructions and sent to a special testing agency for testing. During the test, a reagent tube containing reagents will be used to extract the nucleic acid in the sample.

[0003] CN217324090U discloses a vacuum-sealed pathogen nucleic acid detection cup, comprising a detection device and a detection cup. When in use, a reaction liquid tank is placed in a sampling cabin, the reaction cabin is in a vacuum environment, and the sampling cabin and the reaction cabin are in an isolated state. After sampling and collecting samples, the cap is covered, and the reaction cabin is pushed in the direction of the sampling cabin, so that a through pipe establishes a channel between the sampling cabin and the reaction cabin, and the sample is sucked into the reaction cabin through the negative pressure effect of the reaction cabin. After the sample flows into the reaction cabin, the reaction cabin is reset, and the reaction cabin and the sampling cabin are isolated again. Finally, the infection situation is judged according to the reaction result. The whole process is closed operation to prevent the detection sample from volatilizing into the air, so as to achieve absolute isolation from the air and avoid environmental pollution. The operation is simple and convenient, which provides convenience for the staff and ensures that the detection results are more accurate and effective.

[0004] Although the device can achieve a closed reaction between the reaction solution and the nucleic acid sample, the structure is relatively complex. In actual nucleic acid testing, disposable reagent tubes are generally used to store the reaction solution and samples. The structure of the test cup is relatively complex and is not suitable for one-time use. The cost is too high. Repeated use requires additional disinfection, which will also increase costs.

[0005] In actual sampling, generally a certain number of samples are taken before the entire tray of reagent tubes is placed in a low-temperature storage box for storage. This process takes a certain amount of time. If the ambient temperature is high, the reagent tubes may partially deteriorate, affecting the detection accuracy. Utility Model Content

[0006] In view of the deficiencies in the prior art, the utility model provides a vacuum-sealed pathogen nucleic acid detection device to solve the above-mentioned problems.

[0007] To achieve the above objectives, the utility model is implemented through the following technical solutions: a vacuum-sealed pathogen nucleic acid detection device, comprising:

[0008] Reagent tubes, provided in several groups for storing reaction solutions;

[0009] The storage box is provided with multiple groups, and has multiple vertically connected storage chambers for independently storing reagent tubes;

[0010] A refrigerator box, used to store multiple groups of storage boxes and to take out and put the storage boxes by pulling and pulling, the inner surface of the opening of the refrigerator box is sealed between the storage boxes, and a refrigeration and heat preservation structure is arranged in the refrigerator box;

[0011] The upper and lower ends of the reagent tube are both provided with openable end covers, and the interior of the reagent tube is divided into upper and lower layers by an isolation structure arranged in the middle. The reaction liquid level in the reagent tube is higher than the isolation structure. The isolation structure can be pushed open by a swab to allow the collected sample to be in the reaction liquid in the lower space, and the lower reaction liquid is in a vacuum state through the closed isolation structure and the upper reaction liquid.

[0012] Preferably, the reagent tube comprises:

[0013] The tube body is arranged to be through-through from top to bottom, and a convex ring is arranged in the middle of the inner part;

[0014] The isolation structure is a valve with a cross seam inside, and the valve is adhered to the top of the convex ring.

[0015] Preferably, the end cover comprises a sealing plug inserted into the top end of the tube body for sealing and a sealing cover threadedly connected to the bottom end of the tube body, and one side of the sealing plug is connected to the side surface of the tube body through a flexible plastic belt.

[0016] Preferably, positioning strips are provided on all four walls of the storage cavity of the storage box, the reagent tube can be inserted between the four positioning strips, and the bottom ends of the positioning strips are bent inward to support the reagent tube, a panel with a handle is provided on one side of the storage box, and the panel can cover the opening of the refrigerator, and a magnetic sheet is embedded and pasted on the other side of the storage box for magnetically sucking the storage box into the refrigerator.

[0017] Preferably, multiple groups of top blocks are provided at the top of the refrigerator corresponding to the multiple groups of storage cavities of the storage boxes. The top blocks can be inserted between four positioning strips of the same group to eject the reagent tubes, and the inner side of the bottom of the positioning strip is chamfered. An iron sheet adapted to the position of the magnetic suction sheet is provided in the refrigerator.

[0018] Preferably, a partition for separating a plurality of groups of storage boxes is further provided in the refrigerator, and a handle for lifting the refrigerator is provided on the top of the refrigerator.

[0019] Preferably, the refrigeration and thermal insulation structure includes an insulating lining adhered to the inner wall of the cold storage box, and a semiconductor refrigeration plate arranged at the bottom of the inner surface of the insulating lining. A cooling fan connected to the semiconductor refrigeration plate and dissipating the heat derived therefrom is installed on the outside of the cold storage box. The cooling fan has a built-in power supply and switch, and the iron sheet is adhered to the surface of the insulating lining.

[0020] The utility model provides a vacuum-sealed pathogen nucleic acid detection device. Compared with the prior art, it has the following beneficial effects:

[0021] 1. The vacuum-sealed pathogen nucleic acid detection device uses a refrigerator to store the detection samples, which can achieve the effect of refrigeration and insulation. It is suitable for collecting nucleic acid samples outside for a long time, and uses a drawer-pull-out storage box to store reagent tubes. After a group of nucleic acid samples are placed, one compartment can be pushed in to keep the nucleic acid samples refrigerated in time. At the same time, the outer part of the storage box can continue to hold reagent tubes, which is easy to use. At the same time, the reagent tube itself adopts an upper and lower isolation structure. After the swab is inserted into the lower layer of the valve and stirred and then drawn out, the lower layer reaction liquid can maintain a vacuum-sealed state and does not contact with the air, which can prevent the nucleic acid sample from deteriorating. The upper space can also store the broken swab normally, and the reaction liquid after the reaction can be poured out separately from the lower end, which ensures the detection quality, and the reagent tube has a simple structure and low cost.

[0022] 2. The vacuum-sealed pathogen nucleic acid detection device supports and positions the reagent tubes by providing positioning bars in the storage box, so that the reagent tubes can remain suspended in the air. The top block on the top of the refrigerator facilitates the ejection of a whole row of reagent tubes together, thereby facilitating the extraction of the reagent tubes, thus avoiding the problem of the reagent tubes being deeply hidden in the storage box and being inconvenient to remove, and the operation is labor-saving and convenient.

[0023] 3. The vacuum-sealed pathogen nucleic acid detection device can cool the refrigerator by setting a semiconductor refrigeration plate at the bottom, and the thermal insulation lining can achieve a good thermal insulation effect, ensuring that the refrigerator has a long-term refrigeration effect, avoiding the deterioration of samples, and indirectly ensuring the detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0025] Figure 2 It is a schematic diagram of the storage box of the utility model;

[0026] Figure 3 This is a schematic diagram of the combination of the reagent tube and the positioning strip of the utility model;

[0027] Figure 4 It is a cross-sectional view of the reagent tube of the utility model;

[0028] Figure 5 It is a schematic diagram of the positioning strip of the utility model;

[0029] Figure 6 It is a cross-sectional view of the refrigerator of the present utility model.

[0030] In the figure: 1-reagent tube, 11-tube body, 12-valve, 13-sealing plug, 14-sealing cover, 2-storage box, 21-positioning strip, 22-magnetic sheet, 3-refrigerator, 31-top block, 32-partition, 33-handle, 34-insulation lining, 35-semiconductor refrigeration plate, 36-cooling fan, 37-iron sheet. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0032] The utility model provides three technical solutions:

[0033] Figure 1-Figure 4 The first embodiment is shown: a vacuum-sealed pathogen nucleic acid detection device, comprising:

[0034] Reagent tubes 1, provided with several groups for storing reaction solutions;

[0035] The storage box 2 is provided with multiple groups, and has multiple vertically connected storage cavities for independently storing the reagent tubes 1;

[0036] The refrigerator 3 is used to store multiple groups of storage boxes 2 and to take out and put the storage boxes 2 by pulling. The inner surface of the opening of the refrigerator 3 is sealed between the storage boxes 2, and a refrigeration and heat preservation structure is provided in the refrigerator 3;

[0037] The upper and lower ends of the reagent tube 1 are both provided with openable end covers, and the interior of the reagent tube 1 is divided into upper and lower layers by an isolation structure arranged in the middle. The reaction liquid level in the reagent tube 1 is higher than the isolation structure. The isolation structure can be opened by a swab to allow the collected sample to be in the reaction liquid in the lower space, and the lower reaction liquid is placed in a vacuum state through the closed isolation structure and the upper reaction liquid.

[0038] Reagent tube 1 includes:

[0039] The tube body 11 is provided through the top and bottom, and a convex ring is provided in the middle of the inner part;

[0040] The isolation structure is a valve 12 with a cross seam inside, and the valve 12 is adhered to the top of the convex ring;

[0041] The end cap includes a sealing plug 13 inserted into the top of the tube body 11 for sealing and a sealing cover 14 threadedly connected to the bottom of the tube body 11, and one side of the sealing plug 13 is connected to the side of the tube body 11 through a flexible plastic belt. The connection through the flexible plastic belt can prevent the sealing plug 13 from being lost after being removed, and also makes it possible for the sealing plug 13 not to be placed on the desktop to avoid contamination.

[0042] The refrigerator 3 is used to store the test samples, which can achieve the effect of refrigeration and insulation, and is suitable for collecting nucleic acid samples outside for a long time. The drawer-pull-out storage box 2 is used to store the reagent tube 1. After a group of nucleic acid samples are placed, it can be pushed into a grid to keep the nucleic acid samples refrigerated in time. At the same time, the outer part of the storage box 2 can continue to hold the reagent tube 1, which is easy to use. At the same time, the reagent tube 1 itself adopts an upper and lower isolation structure. After the swab is inserted into the lower layer of the valve 12 and stirred and then pulled out, the lower layer reaction liquid can maintain a vacuum-sealed state and does not contact with the air, which can prevent the nucleic acid sample from deteriorating. The upper space can also store the broken swab normally, and the reaction liquid after the reaction can be poured out separately from the lower end, which ensures the detection quality. The reagent tube 1 has a simple structure and low cost.

[0043] Figure 2-Figure 3 and Figure 5 The second embodiment is shown, which is mainly different from the first embodiment in that: the four walls of the storage cavity of the storage box 2 are all provided with positioning strips 21, the reagent tube 1 can be inserted between the four positioning strips 21, and the bottom end of the positioning strip 21 is bent inward to support the reagent tube 1, a panel with a handle is provided on one side of the storage box 2, and the panel can cover the opening of the refrigerator 3, and a magnetic suction sheet 22 is embedded and pasted on the other side of the storage box 2 for magnetically sucking the storage box 2 in the refrigerator 3 to prevent the storage box 2 from sliding out easily;

[0044] A plurality of top blocks 31 are arranged at the top of the refrigerator 3 corresponding to the plurality of storage cavities of the storage box 2. The top blocks 31 can be inserted between four positioning strips 21 of the same group to push out the reagent tube 1, and a chamfer is arranged on the inner side of the bottom of the positioning strip 21. An iron sheet 37 adapted to the position of the magnetic sheet 22 is arranged in the refrigerator 3.

[0045] By providing a positioning bar 21 in the storage box 2 to support and position the reagent tube 1, the reagent tube 1 can be kept suspended in the air, and by utilizing the top block 31 on the top of the refrigerator 3, the entire row of reagent tubes 1 can be pushed out together, thereby facilitating the extraction of the reagent tube 1, thus avoiding the problem of the reagent tube 1 being deeply hidden in the storage box 2 and being inconvenient to remove, and the operation is labor-saving and convenient.

[0046] Figure 1 and Figure 6The third embodiment is shown, which is mainly different from the second embodiment in that a partition 32 is further provided in the refrigerator 3 to separate the multiple groups of storage boxes 2, and a handle 33 for pulling the refrigerator 3 is provided on the top of the refrigerator 3, and the handle 33 is a flexible belt, which hangs on one side and does not cover the top block 31;

[0047] The refrigeration and heat preservation structure includes a heat preservation lining 34 adhered to the inner wall of the cold storage box 3, and a semiconductor refrigeration plate 35 arranged at the bottom of the inner surface of the heat preservation lining 34. A cooling fan 36 connected to the semiconductor refrigeration plate 35 and dissipating the heat derived therefrom is installed on the outside of the cold storage box 3. The cooling fan 36 has a built-in power supply and a switch, and an iron sheet 37 is adhered to the surface of the heat preservation lining 34.

[0048] By setting a semiconductor refrigeration plate 35 at the bottom, the refrigerator 3 can be cooled, and the thermal insulation lining 34 can achieve a better thermal insulation effect, ensuring that the refrigerator 3 has a long-term refrigeration effect, avoiding the deterioration of the sample, and indirectly ensuring the detection accuracy.

[0049] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art, wherein the model parameters of the heat dissipation fan 36 and the semiconductor refrigeration plate 35 are not specifically limited, and both are products available on the market.

[0050] When in use, start the cooling fan 36 to dissipate heat from the semiconductor refrigeration plate 35, so that the semiconductor refrigeration plate 35 is cooled, pull out a row of storage boxes 2, then take out the reagent tube 1 and open the sealing plug 13, insert the sampled swab into the reagent tube 1, and insert it under the valve 12 for stirring, then withdraw the swab, so that the valve 12 can be automatically restored to its original state, and then break the swab at the tube mouth, and keep the broken part with the cotton ball in the reagent tube 1, and then plug the sealing plug 13 tightly.

[0051] Place the reagent tube 1 into the storage cavity of the storage box 2 close to the refrigerator 3, and then push the storage box 2 inward one grid to move the reagent tube 1 into the refrigerator 3 for storage. Repeat the operation until the storage box 2 is fully pushed in, then pull out the next row of storage boxes 2, until all storage boxes 2 are full of reagent tubes 1, and then the refrigerator 3 can be lifted away as a whole.

[0052] When the reagent tube 1 needs to be taken out, a row of storage boxes 2 is completely pulled out and placed on the top of the refrigerator 3, and the top block 31 is pressed down so that the top block 31 lifts the reagent tube 1 for a while, so that the top of the reagent tube 1 is exposed from the storage box 2, and the reagent tube 1 can be easily taken out. After taking it out, the sealing cover 14 at the bottom can be unscrewed to pour out the reaction liquid in the lower layer.

[0053] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0054] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A vacuum-sealed pathogen nucleic acid detection device, characterized in that: include: Reagent tubes, provided in several groups for storing reaction solutions; The storage box is provided with multiple groups, and has multiple vertically connected storage chambers for independently storing reagent tubes; A refrigerator box, used to store multiple groups of storage boxes and to take out and put the storage boxes by pulling and pulling, the inner surface of the opening of the refrigerator box is sealed between the storage boxes, and a refrigeration and heat preservation structure is arranged in the refrigerator box; The upper and lower ends of the reagent tube are both provided with openable end covers, and the interior of the reagent tube is divided into upper and lower layers by an isolation structure arranged in the middle. The reaction liquid level in the reagent tube is higher than the isolation structure. The isolation structure can be pushed open by a swab to allow the collected sample to be in the reaction liquid in the lower space, and the lower reaction liquid is in a vacuum state through the closed isolation structure and the upper reaction liquid.

2. A vacuum-sealed pathogen nucleic acid detection device according to claim 1, characterized in that: The reagent tube comprises: The tube body is arranged to be through-through from top to bottom, and a convex ring is arranged in the middle of the inner part; The isolation structure is a valve with a cross seam inside, and the valve is adhered to the top of the convex ring.

3. A vacuum-sealed pathogen nucleic acid detection device according to claim 1, characterized in that: The end cover comprises a sealing plug inserted into the top end of the tube body for sealing and a sealing cover threadedly connected to the bottom end of the tube body, and one side of the sealing plug is connected to the side surface of the tube body through a flexible plastic belt.

4. A vacuum-sealed pathogen nucleic acid detection device according to claim 2, characterized in that: The four walls of the storage cavity of the storage box are provided with positioning strips, the reagent tube can be inserted between the four positioning strips, and the bottom ends of the positioning strips are bent inward to support the reagent tube. A panel with a handle is provided on one side of the storage box, and the panel can cover the opening of the refrigerator. A magnetic sheet is embedded and pasted on the other side of the storage box for magnetically sucking the storage box into the refrigerator.

5. A vacuum-sealed pathogen nucleic acid detection device according to claim 4, characterized in that: The top of the refrigerator is provided with multiple groups of top blocks corresponding to the multiple groups of storage cavities of the storage boxes. The top blocks can be inserted between four positioning strips of the same group to push out the reagent tubes, and the bottom inner side of the positioning strip is chamfered. An iron sheet adapted to the position of the magnetic suction sheet is arranged in the refrigerator.

6. A vacuum-sealed pathogen nucleic acid detection device according to claim 1, characterized in that: The refrigerator is also provided with a partition for separating a plurality of groups of storage boxes, and a handle for pulling the refrigerator is provided on the top of the refrigerator.

7. A vacuum-sealed pathogen nucleic acid detection device according to claim 1, characterized in that: The refrigeration and heat preservation structure includes a heat preservation lining adhered to the inner wall of the refrigerator, and a semiconductor refrigeration plate arranged at the bottom of the inner surface of the heat preservation lining. A cooling fan connected to the semiconductor refrigeration plate and dissipating the heat derived from the semiconductor refrigeration plate is installed on the outside of the refrigerator. The cooling fan has a built-in power supply and switch.

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