Layered cell culture bottle
By introducing short longitudinal partition plates, transverse inclined partition plates and inclined tube ports into cell stratified culture bottles, the contact contamination problem caused by a single bottle port is solved, and multiple sets of bottle bodies are realized through silicone rubber column blocks. Safe stacking improves the success rate and safety of cultivation.
Patent Information
- Application Number
- CN202420535261.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-19
- Publication Date
- 2024-12-10
- Estimated Expiration
- 2034-03-19
AI Technical Summary
The existing cell stratified culture flasks are designed with a single bottle opening and a single internal space, which easily leads to contact contamination when injecting the culture liquid and extracting the waste liquid, which in turn affects the success of culture.
A cell-layered culture flask including a short longitudinal partition plate, a transverse inclined partition plate, a silicone rubber column block and an inclined tube mouth were designed to separate the injection and suction ports through the partition plate and the inclined tube mouth structure to prevent the Contact contamination, and achieve safe stacking and anti-slip connection of multiple sets of bottles through silicone rubber column blocks.
It effectively prevents contact pollution, improves the success rate of culture, and through the design of the partition plate and silicone rubber column block, it blocks air impurities and prevents culture failure caused by temperature unevenness, improving the safety and efficiency of culture.
Smart Images
Figure CN222139137U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of culture bottle equipment, in particular to a cell layered culture bottle. Background Art
[0002] A cell stratification culture flask generally refers to a culture flask designed to better simulate the cell growth environment during the cell culture process; existing cell stratification culture flasks are generally designed with a single bottle mouth and a single internal space when in use, and both the injection of culture fluid and the extraction of waste fluid are operated through the single bottle mouth, which may cause contact contamination and thus cause culture failure. Utility Model Content
[0003] The utility model aims to provide a cell stratified culture bottle in order to solve the problem that the existing cell stratified culture bottles are usually designed with a single bottle mouth and a single internal space when in use, and both the injection of culture fluid and the extraction of waste fluid are operated through the single bottle mouth, which may cause contact contamination and thus cause culture failure.
[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell stratified culture bottle, comprising: a culture bottle body, a short longitudinal partition plate fixedly connected to one upper end of the interior of the culture bottle body, a transverse inclined partition plate fixedly connected to one side end of the short longitudinal partition plate, silicone rubber column blocks embedded at the four upper ends of the culture bottle body, column block upper embedding grooves opened at the four upper ends of the culture bottle body, and column block lower embedding grooves opened at the four lower ends of the culture bottle body.
[0005] As a further solution of the utility model: an oblique injection nozzle is fixedly connected to one end of the side of the culture bottle body close to the short longitudinal partition plate, and one end of the oblique injection nozzle is fixedly connected to a injection threaded nozzle.
[0006] As a further solution of the utility model: an oblique pipe opening for liquid suction is fixedly connected to the upper side of one end of the culture bottle body away from the short longitudinal partition plate, and one end of the oblique pipe opening for liquid suction is fixedly connected to a threaded pipe opening for liquid suction.
[0007] As a further solution of the utility model: the short longitudinal partition plate and the transverse inclined partition plate are fixedly connected to the two ends of the inner side of the culture bottle body to divide the interior of the culture bottle body into two spaces, and a through groove structure is provided between the rear end of the transverse inclined partition plate and the rear end of the interior of the culture bottle body.
[0008] As a further solution of the utility model: the number of the silicone rubber column blocks, the column block upper embedding grooves and the column block lower embedding grooves are all set in four groups, and the silicone rubber column blocks can be adapted to be embedded in the column block upper embedding grooves and the column block lower embedding grooves.
[0009] As a further solution of the utility model: the inclined injection nozzle and the injection threaded nozzle are connected to the interior of the culture bottle body and are inclined toward the groove embedded in the column block, and the inclined injection nozzle is threadedly connected to the sealing bottle cap through the injection threaded nozzle.
[0010] As a further solution of the utility model: the inclined pipe opening for liquid suction and the threaded pipe opening for liquid suction are connected to the interior of the culture bottle body and are inclined toward the threaded pipe opening for liquid injection; the inclined pipe opening for liquid suction is threadedly connected to a sealed bottle cap through the threaded pipe opening for liquid suction.
[0011] Compared with the prior art, the beneficial effects of the utility model are:
[0012] The utility model discloses that when waste liquid needs to be sucked out in the late stage of culture, the culture bottle body can be slowly turned over by holding the inclined injection nozzle and one end of the injection thread nozzle so that the waste liquid inside is concentrated on the inclined suction nozzle and one end of the suction thread nozzle, and the turning is continued until the waste liquid passes through the gap between the transverse inclined partition plate and the culture bottle body, and then the waste liquid can be conveniently sucked out by opening the bottle cap at one end of the suction oblique nozzle and the suction thread nozzle. With such a structure, the injection and suction can be separated to prevent contact contamination from causing culture failure, and when sucking liquid, the obliquely arranged transverse inclined partition plate and the suction oblique nozzle can also prevent impurities in the air from directly falling into the culture bottle body and the culture bin to a certain extent, making the use safer and more efficient.
[0013] In the utility model, when multiple groups of culture bottles are used for multiple group culture, the silicone rubber column block can be embedded in the groove embedded in the column block, and the bottle bodies of another group of culture bottles can be inserted into the silicone rubber column block through the groove embedded under the column block below for stacking. Such a structure can achieve a good connection and stacking effect. Compared with the traditional direct stacking, it can effectively separate the gaps between the bottle bodies of multiple groups of culture bottles to prevent the problem of local temperature differences caused by close stacking, which may lead to culture failure. In addition, the silicone rubber column block can not only achieve rapid alignment and stacking, but also have a certain anti-slip buffering effect to prevent the bottles from colliding with each other and being damaged. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 This is a schematic diagram of the overall structure of the cell stratification culture bottle of the utility model;
[0015] Figure 2 It is a schematic diagram of the cross-section internal structure of the cell stratification culture bottle of the utility model;
[0016] Figure 3 It is a schematic diagram of the bottom structure of the cell layered culture bottle of the utility model.
[0017] In the figure: 1. culture bottle body; 2. short longitudinal partition; 3. transverse inclined partition; 4. silicone rubber column block; 5. groove embedded on the column block; 6. groove embedded under the column block; 7. inclined injection nozzle; 8. threaded injection nozzle; 9. inclined aspiration nozzle; 10. threaded aspiration nozzle. DETAILED DESCRIPTION
[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0019] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, which are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as a limitation of the present utility model. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "set" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or it can be the internal connection of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances. The following is an explanation of the embodiments of the present utility model based on the overall structure of the present utility model.
[0020] Reference Figures 1 to 3In the embodiment of the utility model, the cell stratification culture bottle comprises: a culture bottle body 1, a short longitudinal partition plate 2 is fixedly connected to one end of the upper part of the culture bottle body 1, a transverse inclined partition plate 3 is fixedly connected to one end of the side of the short longitudinal partition plate 2, the short longitudinal partition plate 2 and the transverse inclined partition plate 3 are fixedly connected to the two ends of the inner side of the culture bottle body 1 on both sides, the interior of the culture bottle body 1 is divided into two parts of space, a through groove structure is provided between the tail end of the transverse inclined partition plate 3 and the tail end of the interior of the culture bottle body 1, a silicone rubber column block 4 is embedded at the four ends above the culture bottle body 1, a column block upper embedding groove 5 is provided at the four ends above the culture bottle body 1, and a column block lower embedding groove 6 is provided at the four ends below the culture bottle body 1, the silicone rubber column block 4, the column block upper embedding groove 5 and the column block lower embedding groove 6 are all arranged in four groups, and the silicone rubber column block 4 can be adapted to be embedded in the column block upper embedding groove 5 and the column block lower embedding groove 6.
[0021] The above scheme is adopted: such a structure can separate injection and aspiration to prevent contact contamination causing culture failure, and during aspiration, the obliquely arranged transverse inclined partition plate 3 can also prevent impurities in the air from directly falling into the culture bottle body 1 to a certain extent, making the use of the culture chamber safer and more efficient, and when using multiple groups of culture bottles for multiple groups of culture, the silicone rubber column block 4 can be embedded in the groove 5 on the column block, and another group of culture bottle bodies 1 can be inserted into the silicone rubber column block 4 through the groove 6 embedded under the column block below for stacking. Such a structure can achieve a good connection and stacking effect. Compared with traditional direct stacking, it can effectively separate the gaps between multiple groups of culture bottle bodies 1 to prevent the problem of culture failure caused by local temperature differences caused by fitting stacking, and the silicone rubber column block 4 can not only quickly align the stacking, but also have a certain anti-slip buffering effect to prevent the bottles from colliding with each other and being damaged.
[0022] Reference Figures 1 to 3 , an oblique injection nozzle 7 is fixedly connected to one end of the culture bottle body 1 close to the short longitudinal partition plate 2, and an injection threaded nozzle 8 is fixedly connected to one end of the oblique injection nozzle 7. The injection oblique nozzle 7 and the injection threaded nozzle 8 are connected to the interior of the culture bottle body 1 and are inclined toward the direction of the groove 5 embedded on the column block, and the injection oblique nozzle 7 is screwed to the sealed bottle cap through the injection threaded nozzle 8; an aspiration oblique nozzle 9 is fixedly connected to the top of one end of the culture bottle body 1 away from the short longitudinal partition plate 2, and an aspiration oblique nozzle 9 is fixedly connected to one end of the aspiration oblique nozzle 9. The aspiration oblique nozzle 9 and the aspiration threaded nozzle 10 are connected to the interior of the culture bottle body 1 and are inclined toward the injection threaded nozzle 8, and the aspiration oblique nozzle 9 is screwed to the sealed bottle cap through the aspiration threaded nozzle 10.
[0023] Adopting the above scheme: when it is necessary to suck out the waste liquid, hold the inclined injection nozzle 7 and one end of the injection threaded nozzle 8 and slowly flip the culture bottle body 1 to make the internal waste liquid concentrate on the inclined suction nozzle 9 and one end of the suction threaded nozzle 10, and continue to flip until the waste liquid passes through the gap between the transverse inclined partition plate 3 and the culture bottle body 1, and then open the bottle cap at one end of the suction oblique nozzle 9 and the suction threaded nozzle 10 to conveniently carry out the waste liquid suction work.
[0024] The working principle of the utility model is as follows: when in use, the bottle body 1 of the culture bottle 1 with the groove 5 embedded on the column block is first placed upward, the sealed bottle cap at one end of the inclined injection nozzle 7 and the injection threaded nozzle 8 is opened, and the culture liquid is injected into the bottle body 1 of the culture bottle through the inclined injection nozzle 7 to carry out the culture work. When the waste liquid needs to be sucked out in the later stage of the culture, the inclined injection nozzle 7 and the injection threaded nozzle 8 are held and the bottle body 1 of the culture bottle is slowly turned over so that the internal waste liquid is concentrated on the inclined suction nozzle 9 and the suction threaded nozzle 10. The turning is continued until the waste liquid passes through the gap between the transverse inclined partition plate 3 and the bottle body 1 of the culture bottle, and then the waste liquid suction work can be conveniently carried out by opening the bottle cap at one end of the suction nozzle 9 and the suction threaded nozzle 10. Such a structure can separate the injection and suction to prevent contact contamination and cause the culture The culturing failure can be prevented, and during the aspiration, the obliquely arranged transverse inclined partition plate 3 and the oblique aspiration nozzle 9 can also, to a certain extent, prevent impurities in the air from directly falling into the culture bottle body 1, making the use in the culture chamber safer and more efficient. When multiple groups of culture bottles are used for multiple groups of culture, the silicone rubber column block 4 can be embedded in the groove 5 embedded in the column block, and another group of culture bottle bodies 1 can be inserted into the silicone rubber column block 4 through the groove 6 embedded under the column block below for stacking. Such a structure can achieve a good connection and stacking effect. Compared with the traditional direct stacking, it can effectively separate the gaps between the multiple groups of culture bottle bodies 1 to prevent the problem of culturing failure caused by local temperature differences caused by the fitting stacking, and the silicone rubber column block 4 can not only play a role in rapid alignment and stacking, but also have a certain anti-slip buffering effect to prevent the bottles from colliding with each other and being damaged.
[0025] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A cell stratification culture flask, characterized in that: include: A culture bottle body (1), wherein a short longitudinal partition plate (2) is fixedly connected to one upper end of the interior of the culture bottle body (1), a transverse inclined partition plate (3) is fixedly connected to one side end of the short longitudinal partition plate (2), silicone rubber column blocks (4) are embedded at the four upper ends of the culture bottle body (1), column block upper embedding grooves (5) are opened at the four upper ends of the culture bottle body (1), and column block lower embedding grooves (6) are opened at the four lower ends of the culture bottle body (1).
2. The cell stratification culture bottle according to claim 1, characterized in that: An oblique injection nozzle (7) is fixedly connected to one end of the culture bottle body (1) near the short longitudinal partition plate (2), and one end of the oblique injection nozzle (7) is fixedly connected to a threaded injection nozzle (8).
3. The cell stratification culture bottle according to claim 1, characterized in that: An oblique pipe opening (9) for liquid suction is fixedly connected to the upper side of one end of the culture bottle body (1) away from the short longitudinal partition plate (2), and one end of the oblique pipe opening (9) for liquid suction is fixedly connected to a threaded pipe opening (10) for liquid suction.
4. The cell stratification culture bottle according to claim 1, characterized in that: The short longitudinal partition plate (2) and the transverse inclined partition plate (3) are fixedly connected to the two ends of the inner side of the culture bottle body (1) to divide the interior of the culture bottle body (1) into two spaces, and a through groove structure is provided between the rear end of the transverse inclined partition plate (3) and the rear end of the interior of the culture bottle body (1).
5. The cell stratification culture bottle according to claim 1, characterized in that: The silicone rubber column blocks (4), the column block upper embedding grooves (5) and the column block lower embedding grooves (6) are all provided in four groups, and the silicone rubber column blocks (4) can be adapted to be embedded in the column block upper embedding grooves (5) and the column block lower embedding grooves (6).
6. The cell stratification culture bottle according to claim 2, characterized in that: The inclined injection pipe opening (7) and the injection threaded pipe opening (8) are connected to the interior of the culture bottle body (1) and are inclined toward the groove (5) embedded in the column block. The inclined injection pipe opening (7) is threadedly connected to the sealing bottle cap through the injection threaded pipe opening (8).
7. The cell stratification culture bottle according to claim 3, characterized in that: The inclined pipe opening (9) for liquid suction and the threaded pipe opening (10) for liquid suction are connected to the interior of the culture bottle body (1) and are inclined toward the threaded pipe opening (8) for liquid injection. The inclined pipe opening (9) for liquid suction is threadedly connected to a sealed bottle cap through the threaded pipe opening (10).