Amide hydrolase engineering bacterial membrane treatment device

Abstract

The utility model discloses an amide hydrolase engineering bacterial membrane treatment device, which belongs to the technical field of amide hydrolase engineering and comprises a membrane treatment unit, a peristaltic pump unit and a material barrel, the membrane treatment unit is connected with the material barrel through a first hose, and the material barrel is connected with the peristaltic pump unit through a second hose. The peristaltic pump unit is connected with the membrane treatment unit through a third hose; the problems that in the prior art, the labor intensity of workers is large, potential safety hazards exist, and materials are prone to loss can be solved. By opening the peristaltic pump unit, materials enter the peristaltic pump unit from the material barrel and then are added into the feed port of the membrane treatment unit for membrane treatment, and when the material adding speed is too high and the materials reach the liquid leakage port, the materials can flow back into the material barrel from the liquid leakage port, so that the materials are circularly treated until the materials are subjected to membrane treatment. The yield of fermentation liquor can be increased, the material loss is reduced, the potential safety hazard in the operation process is eliminated, and the labor force of personnel in the operation process can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of amide hydrolase engineering, in particular to an amide hydrolase engineering bacterial film processing device. Background Art

[0002] Currently, the fermentation broth of amide hydrolase engineered bacteria needs to undergo membrane experiments to determine the optimized R&D process. The membrane treatment is mainly for purifying the fermentation broth to remove the biological impurities remaining after the intracellular enzyme reaction. The membrane treatment can improve the purity of the main components in the fermentation broth.

[0003] Among them, when the amide hydrolase engineered bacteria fermentation liquid is treated by the membrane, the fermentation liquid is about 50kg each time, while the membrane feed port is only about 12kg. Therefore, each operation requires personnel to add materials continuously, resulting in high labor intensity. Because the membrane equipment is placed on the operating table, the operator needs to raise it to complete the operation, which poses certain safety hazards. At the same time, during the operation, the material may be spilled, causing material loss. Utility Model Content

[0004] The purpose of the utility model is to provide an amide hydrolase engineering biofilm processing device, which can improve the yield of fermentation liquid, reduce material loss, eliminate safety hazards in the operation process, reduce the labor force in the operation process, and solve the problems in the prior art.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] An amide hydrolase engineering bacterial membrane processing device comprises a membrane processing unit, a peristaltic pump unit and a material barrel, wherein the membrane processing unit is connected to the material barrel via a first hose, the material barrel is connected to the peristaltic pump unit via a second hose, and the peristaltic pump unit is connected to the membrane processing unit via a third hose.

[0007] Preferably, the membrane treatment unit comprises a feed bin, and the feed bin is a cylindrical structure with an interlayer.

[0008] Preferably, a feed port is provided on the feed bin, and a liquid leakage port is also provided on the feed bin, and the liquid leakage port is located at the upper end of the feed bin.

[0009] Preferably, the material barrel is provided with a liquid outlet, and the material barrel is also provided with a material inlet.

[0010] Preferably, the material inlet is connected to the inlet end of the peristaltic pump unit through a second hose, and the outlet end of the peristaltic pump unit is connected to the feed port through a third hose.

[0011] Preferably, the liquid leakage port is connected to the liquid outlet via a first hose.

[0012] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0013] The utility model opens the peristaltic pump unit, and the material enters the peristaltic pump unit from the material barrel, and is then added to the feed port of the membrane treatment unit for membrane treatment. When the material is added too quickly, when the material reaches the leakage port, the material will flow back into the material barrel from the leakage port, and the material will be processed in this way in a cycle until the material has passed through the membrane treatment. The yield of the fermentation liquid of the amide hydrolase engineering bacteria fermentation liquid during the membrane process can be improved, material loss can be reduced, safety hazards existing in the operation process can be eliminated, and human labor in the operation process can be reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a structural schematic diagram of the amide hydrolase engineering biofilm treatment device of the utility model;

[0015] Figure 2 It is a structural schematic diagram of the membrane treatment unit of the utility model;

[0016] Figure 3 It is a structural schematic diagram of the material barrel of the utility model.

[0017] In the figure: 1. membrane treatment unit; 11. feed bin; 12. feed port; 13. liquid leakage port; 2. peristaltic pump unit; 3. material barrel; 31. liquid outlet; 32. material inlet; 4. first hose; 5. second hose; 6. third hose. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] In order to solve the existing technical problems that each time the operation is performed, the personnel need to add materials continuously, which leads to high labor intensity. Because the membrane equipment is placed on the operating table, the operator needs to raise it to complete the operation, which has certain safety hazards. At the same time, during the operation, the personnel may spill the materials, causing material loss. Please refer to Figure 1-Figure 3 , this embodiment provides the following technical solutions:

[0020] An amide hydrolase engineering bacterial membrane processing device includes a membrane processing unit 1, a peristaltic pump unit 2 and a material barrel 3, wherein the membrane processing unit 1 is connected to the material barrel 3 via a first hose 4, the material barrel 3 is connected to the peristaltic pump unit 2 via a second hose 5, and the peristaltic pump unit 2 is connected to the membrane processing unit 1 via a third hose 6.

[0021] It should be noted that the material barrel 3 is moved to the membrane processing unit 1 and connected to the peristaltic pump unit 2, so that one end of the second hose 5 is connected to the material inlet 32 ​​at the bottom of the material barrel 3, and the other end of the second hose 5 is connected to the inlet end of the peristaltic pump unit 2, and one end of the third hose 6 is connected to the feed port 12 of the feed bin 11, and the other end of the third hose 6 is connected to the outlet end of the peristaltic pump unit 2, and then the leakage port 13 of the feed bin 11 is connected to the liquid outlet 31 of the material barrel 3 through the first hose 4.

[0022] In this embodiment, the membrane treatment unit 1 comprises a feed bin 11, which is a cylindrical structure with an interlayer.

[0023] In this embodiment, a feed port 12 is provided on the feed bin 11 , and a liquid leakage port 13 is also provided on the feed bin 11 , and the liquid leakage port 13 is located at the upper end of the feed bin.

[0024] It should be noted that the feed port 12 is placed directly above the feed bin 11, and the feed port 12 can be moved according to usage requirements.

[0025] It should be noted that the liquid leakage port 13 is located at 2 / 3 of the height of the feed bin 11 , which can prevent overflow caused by excessively high liquid level in the feed bin 11 .

[0026] In this embodiment, a liquid outlet 31 is provided on the material barrel 3 , and a material inlet 32 ​​is also provided on the material barrel 3 .

[0027] In this embodiment, the material inlet 32 ​​is connected to the inlet end of the peristaltic pump unit 2 through the second hose 5 , and the outlet end of the peristaltic pump unit 2 is connected to the feed port 12 through the third hose 6 .

[0028] It should be noted that, when the peristaltic pump unit 2 is turned on, the material enters the peristaltic pump unit 2 from the material barrel 3 and is then added to the feed port 12 of the membrane treatment unit 1 for membrane treatment.

[0029] In this embodiment, the liquid leakage port 13 is connected to the liquid outlet 31 through the first hose 4 .

[0030] It should be noted that when the material is added too quickly, when the material reaches the position of the leakage port 13, the material will flow back into the material barrel 3 from the leakage port 13, and the material will be processed in a cycle until the material has passed through the membrane.

[0031] Working principle: turn on the peristaltic pump unit 2, the material enters the peristaltic pump unit 2 from the material barrel 3, and is then added to the feed port 12 of the membrane treatment unit 1 for membrane treatment. When the material is added too quickly, when the material reaches the leakage port 13, the material will flow back from the leakage port 13 to the material barrel 3, and the material will be processed in this cycle until the material has passed the membrane treatment.

[0032] It can improve the yield of the fermentation liquid of the amide hydrolase engineering bacteria fermentation liquid during the membrane passing process, reduce material loss, eliminate the safety hazards in the operation process, and reduce the labor force in the operation process.

[0033] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0034] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An amide hydrolase engineering bacterial membrane treatment device, comprising a membrane treatment unit (1), a peristaltic pump unit (2) and a material barrel (3), characterized in that: The membrane processing unit (1) is connected to the material barrel (3) via a first hose (4), the material barrel (3) is connected to the peristaltic pump unit (2) via a second hose (5), and the peristaltic pump unit (2) is connected to the membrane processing unit (1) via a third hose (6).

2. The amide hydrolase engineering bacterial membrane treatment device according to claim 1, characterized in that: The membrane treatment unit (1) comprises a feed bin (11), and the feed bin (11) is a cylindrical structure with an interlayer.

3. The amide hydrolase engineering bacterial membrane treatment device according to claim 2, characterized in that: The feed bin (11) is provided with a feed port (12), and the feed bin (11) is also provided with a liquid leakage port (13), and the liquid leakage port (13) is located at the upper end of the feed bin.

4. The amide hydrolase engineering bacterial membrane treatment device according to claim 3, characterized in that: The material barrel (3) is provided with a liquid outlet (31), and the material barrel (3) is also provided with a material inlet (32).

5. The amide hydrolase engineering bacterial membrane treatment device according to claim 4, characterized in that: The material inlet (32) is connected to the inlet end of the peristaltic pump unit (2) via a second hose (5), and the outlet end of the peristaltic pump unit (2) is connected to the feed port (12) via a third hose (6).

6. The amide hydrolase engineering bacterial membrane treatment device according to claim 5, characterized in that: The liquid leakage port (13) is connected to the liquid outlet (31) via a first hose (4).

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