Stem cell and immune cell freezing system

Abstract

The utility model provides a stem cell and immune cell freezing system, belongs to the technical field of stem cell and immune cell freezing storage, and aims to solve the problem that stem cells and immune cells which are not taken out from a freezing box are unfrozen. Comprising a freezer; a freezing structure is fixedly arranged on the outer side of the freezing box, and a locking device is fixedly arranged on the outer side of the top of the freezing box. A containing assembly is fixedly arranged in the freezing box, and the top of the containing assembly is fixedly arranged on a connecting assembly. A sliding disc, a sealing groove C and a sealing strip B are arranged; when a group of reagent tubes filled with stem cells or immune cells are taken, other reagent tubes filled with stem cells or immune cells are still in a freezing environment; furthermore, all stem cells and immune cells in the freezing box can be prevented from being in contact with outside air; the unfreezing phenomenon of the stem cells and the immune cells which are not taken out from the freezing box can be prevented.

Description

Technical Field

[0001] The utility model belongs to the technical field of stem cell and immune cell freezing storage, and more specifically, particularly relates to a stem cell and immune cell freezing system. Background Art

[0002] Stem cell and immune cell freezing is the process of isolating and culturing stem cells and immune cells from different human tissues, testing and identifying them, and then freezing them at deep low temperatures so that they can be revived and re-infused into patients when clinically needed, in order to achieve the purpose of treating the disease.

[0003] Existing stem cells and immune cells are usually cryopreserved in a cryopreservation box. Currently, the cryopreservation box generally has an opening on the top and a box lid on the outside of the opening. When the box lid is opened, all the stem cells and immune cells in the cryopreservation box will come into contact with the outside air. This may cause the stem cells and immune cells that have not been removed from the cryopreservation box to thaw, which may cause a certain degree of damage to the stem cells and immune cells, reducing the practicality of the cryopreservation box. Utility Model Content

[0004] In order to solve the above technical problems, the utility model provides a stem cell and immune cell freezing system to solve the problem raised in the above background technology that the stem cells and immune cells not taken out of the freezer may be thawed, which may cause a certain degree of damage to the stem cells and immune cells.

[0005] The utility model provides a stem cell and immune cell freezing system, which is achieved by the following specific technical means:

[0006] A stem cell and immune cell freezing system, comprising a freezing box; a sealing strip A is fixedly arranged inside the top side of the freezing box, a box cover is movably arranged on the top of the freezing box, and a handle is fixedly arranged on the top of the box cover; a sealing groove A is opened inside the bottom side of the box cover, a sealing strip A is movably arranged inside the sealing groove A, and four groups of fixing hooks are symmetrically fixedly arranged on the outside of the box cover; a freezing structure is fixedly arranged on the outside of the freezing box, and a locking device is fixedly arranged on the outside of the top of the freezing box; a containing assembly is fixedly arranged inside the freezing box, and the top of the containing assembly is fixedly arranged on the connecting assembly;

[0007] A partition is fixedly arranged inside the freezer, and a sealing groove C is arranged in a straight line at the bottom of the partition; a sealing groove B is arranged in a straight line inside the partition, and a connecting groove is arranged in a straight line inside the top side of the partition; connecting holes are opened inside the partition, and the connecting holes are located on both sides of the connecting groove; the freezing structure includes: a liquid storage tank, a liquid outlet pipe and a liquid pump; the liquid storage tank is fixedly arranged outside the freezer, and liquid nitrogen is stored in the liquid storage tank; one end of the liquid outlet pipe is fixedly arranged inside the bottom side of the liquid storage tank; the liquid pump is fixedly arranged outside the freezer, and a fixed connection is arranged between the liquid inlet of the liquid pump and the other end of the liquid outlet pipe.

[0008] In at least some embodiments, a temperature sensing probe is fixedly disposed on the inner bottom of the freezer, and a control module is fixedly disposed on the outer side of the freezer, and the control module is electrically connected to the temperature sensing probe and the liquid pump.

[0009] In at least some embodiments, the freezing structure also includes: a fixed tube and a nozzle; the fixed tube is fixedly arranged inside the freezer, and one end of the fixed tube is fixedly connected to the liquid outlet of the liquid pump; the nozzle is fixedly arranged in a straight line inside one side of the fixed tube.

[0010] In at least some embodiments, the locking device includes: a locking seat, a handle, a fixed rod, a sliding block and a locking ring; the locking seat is fixedly arranged on the outside of the freezer; one end of the handle is rotatably arranged on the inside of the locking seat; the fixed rod is fixedly arranged inside the handle; the sliding block is slidably arranged between the inside of the handle and the outside of the fixed rod, and a spring member is arranged between the top of the sliding block and the top of the fixed rod; the locking ring is rotatably arranged on the outside of the sliding block, and the locking ring is movably arranged on the inside of the fixed hook.

[0011] In at least some embodiments, the containing assembly includes: a guide vertical rod, a sliding plate, a sealing strip B, a vertical plate, a fixing ring and a sealing strip C; the guide vertical rod is fixedly arranged between the bottom of the partition and the inside of the freezer; the sliding plate is slidably arranged on the outside of the guide vertical rod, and a spring member is arranged between the bottom of the sliding plate and the bottom end of the guide vertical rod; the sealing strip B is fixedly arranged on the top of the sliding plate, and the sealing strip B is movably arranged inside the sealing groove C; the vertical plate is fixedly arranged on the top of the sliding plate in a circular array; the fixing ring is fixedly arranged on the top of the vertical plate; the sealing strip C is fixedly arranged on the outside of the fixing ring, and the sealing strip C is movably arranged inside the sealing groove B.

[0012] In at least some embodiments, the containing assembly further includes: a fixed seat, an auxiliary seat and an elastic plate; the fixed seat is fixedly disposed at the inner bottom of the vertical plate; the auxiliary seat is slidably disposed at the inner top of the vertical plate through a dovetail groove; the elastic plate is rotatably disposed between the fixed seat and the auxiliary seat, and the elastic plate is configured as an arc structure.

[0013] In at least some embodiments, the connecting component includes: a threaded column, a connecting frame, a connecting block, an auxiliary plate and a handle; the threaded column is arranged inside the fixing ring through a threaded connection; the connecting frame is fixedly arranged at the top of the threaded column, and the bottom of the connecting frame is in contact with the top of the fixing ring, and the connecting frame is movably arranged inside the connecting groove; the connecting block is slidably arranged inside the two sides of the connecting frame, and one side of the bottom of the connecting block is arranged as a bevel structure; a spring member is arranged between the connecting block and the inside of the connecting frame, and the connecting block is slidably arranged inside the connecting hole; the auxiliary plate is fixedly arranged on the top of the connecting block, and the auxiliary plate is slidably arranged inside the top side of the connecting frame; the handle is fixedly arranged on the top of the connecting frame.

[0014] Compared with the prior art, the utility model has the following beneficial effects:

[0015] 1. In the utility model, by providing a sliding plate, a sealing groove C, a sealing strip B and a partition, when taking out a group of reagent tubes containing stem cells or immune cells, the remaining reagent tubes containing stem cells or immune cells can remain in a frozen environment, thereby preventing all stem cells and immune cells in the freezer from contacting with the outside air, which is beneficial to preventing the stem cells and immune cells that have not been taken out of the freezer from thawing.

[0016] 2. In the utility model, by providing a partition and a box cover, the top of the freezer can be double-sealed, which is beneficial to ensuring the sealing effect and the freezing preservation effect; by providing an elastic plate, the elasticity of the annular array elastic plate can be used to clamp and fix the reagent tubes containing stem cells and immune cells, which can prevent the reagent tubes from tipping over or shaking when the freezer is moved, thereby enhancing the practicality of the freezer.

[0017] 3. In the utility model, by providing a spring member between the bottom of the sliding plate and the bottom end of the guide vertical rod, the spring member can automatically push out the reagent tube containing stem cells and immune cells through the sliding plate, so that the reagent tube is located above the partition, which is conducive to taking and placing the reagent tube and enhancing the convenience of the freezer. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0019] Figure 2 It is a cross-sectional structural schematic diagram of the freezer box of the utility model.

[0020] Figure 3 It is a schematic diagram of the internal structure of the freezer of the utility model.

[0021] Figure 4 It is a structural schematic diagram of the lower surface of the box cover of the utility model.

[0022] Figure 5It is a structural schematic diagram of the upper surface of the sliding disk of the utility model.

[0023] Figure 6 It is a structural schematic diagram of the lower surface of the partition of the utility model.

[0024] Figure 7 It is a structural schematic diagram of the containing component of the utility model.

[0025] Figure 8 It is a structural schematic diagram of the connection component of the utility model.

[0026] In the figure, the corresponding relationship between the component names and the figure numbers is as follows:

[0027] 1. Freezer; 101. Sealing strip A; 102. Box cover; 103. Sealing groove A; 104. Handle; 105. Fixing hook; 106. Temperature sensor probe; 107. Control module; 108. Partition plate; 109. Sealing groove B; 1010. Sealing groove C; 1011. Connecting groove; 1012. Connecting hole;

[0028] 2. Refrigeration structure; 201. Liquid storage tank; 202. Liquid outlet pipe; 203. Liquid pump; 204. Fixed pipe; 205. Sprinkler;

[0029] 3. Locking device; 301. Locking seat; 302. Handle; 303. Fixed rod; 304. Sliding block; 305. Locking ring;

[0030] 4. Container assembly; 401. Guide vertical rod; 402. Sliding plate; 403. Sealing strip B; 404. Vertical plate; 405. Fixing ring; 406. Sealing strip C; 407. Fixing seat; 408. Auxiliary seat; 409. Elastic plate;

[0031] 5. Connecting assembly; 501. Threaded column; 502. Connecting frame; 503. Connecting block; 504. Auxiliary plate; 505. Handle. DETAILED DESCRIPTION

[0032] The implementation of the utility model is further described in detail below in conjunction with the drawings and examples.

[0033] Embodiment 1:

[0034] As attached Figure 1 To Attachment Figure 8 As shown:

[0035] The utility model provides a stem cell and immune cell freezing system, comprising a freezing box 1; a sealing strip A101 is fixedly arranged inside the top side of the freezing box 1, and a box cover 102 is movably arranged on the top of the freezing box 1, and a handle 104 is fixedly arranged on the top of the box cover 102; a sealing groove A103 is opened inside the bottom side of the box cover 102, and a sealing strip A101 is movably arranged inside the sealing groove A103, and four groups of fixing hooks 105 are symmetrically fixedly arranged on the outer side of the box cover 102; a freezing structure 2 is fixedly arranged on the outer side of the freezing box 1, and a locking device 3 is fixedly arranged on the outer side of the top of the freezing box 1; a containing component 4 is fixedly arranged inside the freezing box 1, and the top of the containing component 4 is fixedly arranged on the connecting component 5;

[0036] In the disclosed embodiment, a partition 108 is fixedly arranged inside the freezer 1, and a sealing groove C1010 is arranged in a straight line at the bottom of the partition 108; a sealing groove B109 is arranged in a straight line inside the partition 108, and a connecting groove 1011 is arranged in a straight line inside the top side of the partition 108; a connecting hole 1012 is opened inside the partition 108, and the connecting hole 1012 is located on both sides of the connecting groove 1011; the freezing structure 2 includes: a liquid storage tank 201, a liquid outlet pipe 202, a liquid pump 203, a fixed pipe 204 and a nozzle 205 ; Liquid storage tank 201 is fixedly arranged outside the freezing box 1, and liquid nitrogen is stored inside the liquid storage tank 201; one end of the liquid outlet pipe 202 is fixedly arranged inside the bottom side of the liquid storage tank 201; liquid pump 203 is fixedly arranged outside the freezing box 1, and the liquid inlet of the liquid pump 203 and the other end of the liquid outlet pipe 202 are arranged to be fixedly connected; fixed pipe 204 is fixedly arranged inside the freezing box 1, and one end of the fixed pipe 204 is fixedly connected to the liquid outlet of the liquid pump 203; nozzles 205 are fixedly arranged inside one side of the fixed pipe 204 in a linear arrangement; the bottom of the inner side of the freezing box 1 A temperature sensing probe 106 is fixedly arranged, and a control module 107 is fixedly arranged on the outside of the freezer 1, and the control module 107 is electrically connected to the temperature sensing probe 106 and the liquid pump 203; the locking device 3 includes: a locking seat 301, a handle 302, a fixed rod 303, a sliding block 304 and a locking ring 305; the locking seat 301 is fixedly arranged on the outside of the freezer 1; one end of the handle 302 is rotatably arranged on the inside of the locking seat 301; the fixed rod 303 is fixedly arranged inside the handle 302; the sliding block 304 is slidably arranged A spring member is arranged between the inside of the handle 302 and the outside of the fixed rod 303, and between the top of the sliding block 304 and the top of the fixed rod 303; the locking ring 305 is rotatably arranged on the outside of the sliding block 304, and the locking ring 305 is movably arranged on the inside of the fixed hook 105; its specific function is: by arranging the sliding plate 402 and the sealing groove C1010 and the sealing strip B403 and the partition 108; when taking a group of reagent tubes containing stem cells or immune cells, the remaining reagent tubes containing stem cells or immune cells can remain in a frozen environment.

[0037] Embodiment 2:

[0038] As attached Figure 3 , Attachment Figure 5 With attached Figure 7 As shown: Based on the first embodiment, the containing assembly 4 includes: a guide vertical rod 401, a sliding plate 402, a sealing strip B403, a vertical plate 404, a fixing ring 405, a sealing strip C406, a fixing seat 407, an auxiliary seat 408 and an elastic plate 409; the guide vertical rod 401 is fixedly arranged between the bottom of the partition 108 and the inside of the freezer 1; the sliding plate 402 is slidably arranged on the outside of the guide vertical rod 401, and a spring member is arranged between the bottom of the sliding plate 402 and the bottom end of the guide vertical rod 401; the sealing strip B403 is fixedly arranged on the top of the sliding plate 402, and the sealing strip B403 is movably arranged inside the sealing groove C1010; the vertical plate 404 is fixedly arranged on the top of the sliding plate 402 in an annular array; The fixing ring 405 is fixedly arranged on the top of the vertical plate 404; the sealing strip C406 is fixedly arranged on the outside of the fixing ring 405, and the sealing strip C406 is movably arranged inside the sealing groove B109; the fixing seat 407 is fixedly arranged on the inner bottom of the vertical plate 404; the auxiliary seat 408 is slidably arranged on the inner top of the vertical plate 404 through a dovetail groove; the elastic plate 409 is rotatably arranged between the fixing seat 407 and the auxiliary seat 408, and the elastic plate 409 is arranged as an arc structure; its specific function is: by arranging a spring part between the bottom of the sliding disk 402 and the bottom end of the guide vertical rod 401, the spring part can automatically push out the reagent tube containing stem cells and immune cells through the sliding disk 402, which is conducive to taking and placing the reagent tube.

[0039] Embodiment three:

[0040] As attached Figure 5 With attached Figure 8As shown: on the basis of the first and second embodiments, the connection assembly 5 includes: a threaded column 501, a connection frame 502, a connection block 503, an auxiliary plate 504 and a handle 505; the threaded column 501 is arranged inside the fixing ring 405 through a threaded connection; the connection frame 502 is fixedly arranged on the top of the threaded column 501, and the bottom of the connection frame 502 is in contact with the top of the fixing ring 405, and the connection frame 502 is movably arranged inside the connection groove 1011; the connection block 503 is slidably arranged inside the two sides of the connection frame 502, and one side of the bottom of the connection block 503 is set as a bevel structure; the connection block 503 and the connection A spring member is arranged between the inside of the frame 502, and the connecting block 503 is slidably arranged inside the connecting hole 1012; the auxiliary plate 504 is fixedly arranged on the top of the connecting block 503, and the auxiliary plate 504 is slidably arranged inside the top side of the connecting frame 502; the handle 505 is fixedly arranged on the top of the connecting frame 502; its specific function is: by providing the connecting block 503, the connecting hole 1012 and the spring member, the connecting frame 502 can be quickly fixed in the connecting groove 1011, which is conducive to placing the reagent tube containing stem cells and immune cells in the freezing space between the inner bottom of the freezer 1 and the bottom of the partition 108.

[0041] The specific usage and function of this embodiment are as follows:

[0042] In the present invention, when in use, the reagent tube containing stem cells and immune cells is pressed into the inner side of the elastic plate 409; the elastic plate 409 uses its own elasticity to clamp and fix the reagent tube on the inner side of the vertical plate 404; then the threaded column 501 is screwed into the interior of the fixing ring 405 by means of a threaded connection; then the connecting frame 502 is pressed down by the handle 505 to press the reagent tube down to the bottom of the partition 108; when the connecting frame 502 slides into the connecting groove 1011, the partition 108 squeezes the connecting block 503, and the connecting block 503 slides into the interior of the connecting frame 502 through the bottom bevel. When the sealing strip When C406 slides into the sealing groove B109, the spring member drives the connecting block 503 to slide into the connecting hole 1012, so that the connecting frame 502 is fixed inside the connecting groove 1011; so that the reagent tube containing stem cells and immune cells is placed between the partition 108 and the inner bottom of the freezing box 1; then the liquid nitrogen in the liquid storage tank 201 is pumped into the fixed tube 204 through the liquid pump 203 and the liquid outlet pipe 202, so that the liquid nitrogen is sprayed into the space between the inner bottom of the freezing box 1 and the bottom of the partition 108 through the nozzle 205, so that the reagent tube containing stem cells and immune cells is frozen. The temperature sensor probe 107 detects the temperature of the space between the bottom of the inner side of the freezer 1 and the bottom of the partition 108. When the temperature reaches a predetermined value, the temperature sensor probe 107 transmits a temperature signal to the control module 106, and the control module 106 controls the liquid pump 203 to stop working. At the same time, the box cover 102 is placed on the top of the freezer 1, and the locking ring 305 is placed inside the fixing hook 105, and the handle 302 is pressed down to make the handle 302 in a vertical state, so that the sliding block 304 pulls down the locking ring 305 through the spring member, thereby making the locking ring 305 The box cover 102 is fixed by the fixing hook 105; when a group of reagent tubes needs to be taken out, the box cover 102 is opened; then the connecting block 503 is pulled out from the connecting hole 1012 through the auxiliary plate 504, so that the sliding plate 402 and the spring member at the bottom of the guide vertical rod 401 push the sliding plate 402 out, so that the sealing strip B403 slides into the sealing groove C1010; the reagent tubes are automatically popped out; and because of the provision of the partition 108 and the sliding plate 402 as well as the sealing strip B403 and the sealing groove C1010, the remaining two groups of reagent tubes are still in a freezing environment.

Claims

1. A stem cell and immune cell freezing system, characterized in that: include: A freezer (1); a sealing strip A (101) is fixedly arranged inside the top side of the freezer (1), a box cover (102) is movably arranged on the top of the freezer (1), and a handle (104) is fixedly arranged on the top of the box cover (102); a sealing groove A (103) is opened inside the bottom side of the box cover (102), a sealing strip A (101) is movably arranged inside the sealing groove A (103), and four groups of fixing hooks (105) are symmetrically fixedly arranged on the outside of the box cover (102); a freezing structure (2) is fixedly arranged on the outside of the freezer (1), and a locking device (3) is fixedly arranged on the outside of the top of the freezer (1); a containing assembly (4) is fixedly arranged inside the freezer (1), and the top of the containing assembly (4) is fixedly arranged on the connecting assembly (5); The freezer (1) is fixedly provided with a partition (108) inside, and a sealing groove C (1010) is provided at the bottom of the partition (108) in a straight line arrangement; a sealing groove B (109) is provided inside the partition (108) in a straight line arrangement, and a connecting groove (1011) is provided inside the top side of the partition (108) in a straight line arrangement; a connecting hole (1012) is provided inside the partition (108), and the connecting hole (1012) is located on both sides of the connecting groove (1011); The freezing structure (2) comprises: a liquid storage tank (201), a liquid outlet pipe (202) and a liquid pump (203); the liquid storage tank (201) is fixedly arranged outside the freezing box (1), and liquid nitrogen is stored inside the liquid storage tank (201); one end of the liquid outlet pipe (202) is fixedly arranged inside the bottom side of the liquid storage tank (201); the liquid pump (203) is fixedly arranged outside the freezing box (1), and a liquid inlet of the liquid pump (203) and the other end of the liquid outlet pipe (202) are arranged to be fixedly connected.

2. A stem cell and immune cell freezing system according to claim 1, characterized in that: A temperature sensing probe (106) is fixedly arranged on the bottom of the inner side of the freezer (1), and a control module (107) is fixedly arranged on the outer side of the freezer (1), and the control module (107) is electrically connected to the temperature sensing probe (106) and the liquid pump (203).

3. A stem cell and immune cell freezing system according to claim 1, characterized in that: The freezing structure (2) further comprises: a fixed pipe (204) and a nozzle (205); the fixed pipe (204) is fixedly arranged inside the freezing box (1), and one end of the fixed pipe (204) is fixedly connected to the liquid outlet of the liquid pump (203); the nozzle (205) is fixedly arranged in a linear manner inside one side of the fixed pipe (204).

4. A stem cell and immune cell freezing system according to claim 1, characterized in that: The locking device (3) comprises: a locking seat (301), a handle (302), a fixing rod (303), a sliding block (304) and a locking ring (305); the locking seat (301) is fixedly arranged on the outside of the freezer (1); one end of the handle (302) is rotatably arranged on the inside of the locking seat (301); the fixing rod (303) is fixedly arranged inside the handle (302); the sliding block (304) is slidably arranged between the inside of the handle (302) and the outside of the fixing rod (303), and a spring member is arranged between the top of the sliding block (304) and the top of the fixing rod (303); the locking ring (305) is rotatably arranged on the outside of the sliding block (304), and the locking ring (305) is movably arranged on the inside of the fixing hook (105).

5. A stem cell and immune cell freezing system according to claim 1, characterized in that: The containing assembly (4) comprises: a guide vertical rod (401), a sliding plate (402), a sealing strip B (403), a vertical plate (404), a fixing ring (405) and a sealing strip C (406); the guide vertical rod (401) is fixedly arranged between the bottom of the partition (108) and the inside of the freezer (1); the sliding plate (402) is slidably arranged outside the guide vertical rod (401), and a spring is arranged between the bottom of the sliding plate (402) and the bottom end of the guide vertical rod (401). The invention relates to a sealing member; a sealing strip B (403) is fixedly arranged on the top of the sliding plate (402), and the sealing strip B (403) is movably arranged inside the sealing groove C (1010); the vertical plates (404) are fixedly arranged on the top of the sliding plate (402) in an annular array; the fixing ring (405) is fixedly arranged on the top of the vertical plates (404); the sealing strip C (406) is fixedly arranged on the outside of the fixing ring (405), and the sealing strip C (406) is movably arranged inside the sealing groove B (109).

6. A stem cell and immune cell freezing system according to claim 5, characterized in that: The containing assembly (4) further comprises: a fixed seat (407), an auxiliary seat (408) and an elastic plate (409); the fixed seat (407) is fixedly arranged at the inner bottom of the vertical plate (404); the auxiliary seat (408) is slidably arranged at the inner top of the vertical plate (404) through a dovetail groove; the elastic plate (409) is rotatably arranged between the fixed seat (407) and the auxiliary seat (408), and the elastic plate (409) is arranged as an arc structure.

7. A stem cell and immune cell freezing system according to claim 5, characterized in that: The connection assembly (5) comprises: a threaded column (501), a connection frame (502), a connection block (503), an auxiliary plate (504) and a handle (505); the threaded column (501) is arranged inside the fixing ring (405) through a threaded connection; the connection frame (502) is fixedly arranged on the top of the threaded column (501), and the bottom of the connection frame (502) is in contact with the top of the fixing ring (405), and the connection frame (502) is movably arranged inside the connection groove (1011); the connection block (503) ) are slidably arranged inside the two sides of the connecting frame (502), and one side of the bottom of the connecting block (503) is arranged as a bevel structure; a spring member is arranged between the connecting block (503) and the inside of the connecting frame (502), and the connecting block (503) is slidably arranged inside the connecting hole (1012); the auxiliary plate (504) is fixedly arranged on the top of the connecting block (503), and the auxiliary plate (504) is slidably arranged inside the top side of the connecting frame (502); the handle (505) is fixedly arranged on the top of the connecting frame (502).

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