Full-automatic plasmid small purification instrument

Abstract

The utility model provides a full-automatic small plasmid purification instrument which comprises a shell part, a first lifting device, a horizontal moving device and a tray are arranged in the shell part, a pipettor is connected onto the first lifting device, a pore plate seat for placing a pore plate is arranged on the horizontal moving device, and the horizontal moving device drives the pore plate seat to move below the pipettor; the tray is arranged on the tray bearing frame and is positioned below the pore plate seat moving area; a pushing device used for pushing a tray is arranged at one end of the pore plate seat, a tray outlet corresponding to the tray is formed in the shell component, a bin door plate is arranged on the outer side of the tray outlet, and the upper side of the bin door plate is hinged to the shell component. According to the utility model, the tray is arranged below the moving area of the pore plate seat and is used for receiving the dropped reagent, so that the reagent is prevented from dropping into equipment and polluting the equipment.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a full-automatic plasmid small extraction and purification instrument. Background Art

[0002] At present, the extraction and purification of Escherichia coli plasmid DNA requires steps such as injection, oscillation, magnetic bead extraction, washing, and elution. The pure plasmid DNA after extraction can be directly used for applications such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection.

[0003] At present, when plasmid purification equipment is performing plasmid extraction and purification operations, the pipette inside the plasmid purification equipment needs to repeatedly transfer reagents. During the process of transferring the reagents, if the pipette is improperly operated or the pipette malfunctions, the reagents can easily drip into the equipment, thereby contaminating the equipment and making it more troublesome to clean up. Summary of the invention

[0004] The utility model aims to solve the deficiencies in the prior art and provide a fully automatic plasmid small extraction and purification instrument.

[0005] The purpose of the utility model is achieved through the following technical solutions: a fully automatic plasmid small extraction and purification instrument, including a shell component, a first lifting device, a horizontal moving device, and a tray are arranged in the shell component, a pipette is connected to the first lifting device, a well plate seat for placing a well plate is arranged on the horizontal moving device, and the horizontal moving device drives the well plate seat to move under the pipette;

[0006] The pallet is placed on the pallet carrier, and the pallet is located below the moving area of ​​the orifice plate seat; a pushing device for pushing the pallet is provided at one end of the orifice plate seat, a pallet outlet corresponding to the pallet is provided on the outer shell component, a door plate is provided on the outer side of the pallet outlet, and the upper side of the door plate is hinged to the outer shell component.

[0007] In the utility model, a tray is arranged below the moving area of ​​the orifice plate seat, and the tray is used to catch the dripping reagent to prevent the reagent from dripping into the inside of the equipment and causing pollution to the equipment; when it is necessary to take out the tray, one end of the tray is pushed by the pushing device, and after the tray is pushed by the pushing device, the other end of the tray will push open the compartment door plate and extend from the tray outlet, and the tray automatically pushes the compartment door plate open, which makes it convenient for the operator to take out the tray, and the pushing device pushes the tray out and then resets it; when it is necessary to load the tray into the equipment, it is only necessary to open the compartment door plate first, and then load the tray into the equipment from the tray outlet, and place the tray on the tray carrier; in this way, the removal and loading of the tray are convenient.

[0008] Preferably, the pushing device is an electric cylinder.

[0009] Preferably, the electric cylinder is provided with a retractable push rod, and a rubber head is provided at the front end of the push rod.

[0010] Preferably, the pushing device is arranged on a fixing frame.

[0011] Preferably, the tray carrier is provided with a guide groove area, the tray is placed in the guide groove area on the tray carrier, and the tray can move along the length direction of the guide groove area.

[0012] Preferably, the tray carrier is provided with a magnet, and the lower end of the tray is provided with a magnetic attraction piece corresponding to the magnet. When the tray is loaded in place, the magnetic attraction piece at the lower end of the tray and the magnet on the tray carrier attract each other, thereby achieving the positioning of the tray.

[0013] Preferably, the magnetic attraction member is made of iron.

[0014] Preferably, a second lifting device is provided in the outer shell component, and a magnetic sleeve fixing frame and a magnetic rod fixing frame are connected to the second lifting device. A plurality of magnetic sleeves are provided at the lower end of the magnetic sleeve fixing frame, and a plurality of magnetic rods are provided at the lower end of the magnetic rod fixing frame. The magnetic rods are correspondingly arranged above the magnetic sleeves; and a cavity into which the magnetic rods can be inserted is provided on the magnetic sleeves.

[0015] The beneficial effects of the utility model are as follows: in the utility model, a tray is arranged below the moving area of ​​the orifice plate seat, and the tray is used to catch dripping reagents to prevent the reagents from dripping into the interior of the equipment and causing pollution to the equipment; when the tray needs to be taken out, one end of the tray is pushed by a pushing device, and after the tray is pushed by the pushing device, the other end of the tray will push open the compartment door plate and extend from the tray outlet, which makes it convenient for the operator to take out the tray. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the utility model.

[0017] Figure 2 It is a schematic diagram of the internal structure of the utility model.

[0018] Figure 3 This is a schematic diagram of the internal structure of the utility model after the tray is removed.

[0019] Figure 4 for Figure 3 Enlarged view of the middle-A part.

[0020] In the figure: 1, shell component, 2, compartment door plate, 3, first lifting device, 4, second lifting device, 5, pipette, 6, magnetic rod fixing frame, 7, magnetic rod, 8, magnetic sleeve fixing frame, 9, magnetic sleeve, 10, horizontal moving device, 11, orifice plate seat, 12, orifice plate, 13, tray carrying frame, 14, tray, 15, electric cylinder, 16, fixing frame, 17, magnet, 18, push rod, 19, rubber head. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.

[0022] like Figures 1 to 4 As shown, a fully automatic plasmid small extraction and purification instrument comprises a housing component 1, a first lifting device 3 and a horizontal moving device 10 are arranged in the housing component 1, a pipette 5 is connected to the first lifting device 3, and the pipette 5 is driven to move up and down by the first lifting device 3. A well plate seat 11 for placing a well plate 12 is arranged on the horizontal moving device 10, and the well plate 12 is placed on the well plate seat 11, and the horizontal moving device 10 drives the well plate seat 11 to move below the pipette 5.

[0023] A second lifting device 4 is provided in the housing component 1, and a magnetic sleeve fixing frame 8 and a magnetic rod fixing frame 6 are connected to the second lifting device 4. The magnetic sleeve fixing frame 8 and the magnetic rod fixing frame 6 are driven to move up and down by the second lifting device 4, and the magnetic sleeve fixing frame 8 and the magnetic rod fixing frame 6 can move independently. There are a plurality of magnetic sleeves 9 at the lower end of the magnetic sleeve fixing frame 8, and a plurality of magnetic rods 7 are provided at the lower end of the magnetic rod fixing frame 6, and the magnetic rods 7 are correspondingly arranged above the magnetic sleeves 9. The magnetic sleeve 9 is provided with a cavity into which the magnetic rods 7 can be inserted.

[0024] The tray 14 is placed on the tray carrier 13, and the tray 14 is located below the moving area of ​​the orifice plate seat 11. The tray carrier 13 is provided with a guide groove area, and the tray 14 is placed in the guide groove area on the tray carrier 13, and the tray 14 can move along the length direction of the guide groove area. A pushing device for pushing the tray 14 is provided at one end of the orifice plate seat 11, and the pushing device is arranged on the fixing frame 16. In this embodiment, the pushing device is an electric cylinder 15. A retractable push rod 18 is provided on the electric cylinder 15, and a rubber head 19 is provided at the front end of the push rod 18.

[0025] The tray carrier 13 is provided with a magnet 17, and the lower end of the tray 14 is provided with a magnetic attraction piece corresponding to the magnet 17, and the magnetic attraction piece is made of iron. When the tray 14 is loaded into place, the magnetic attraction piece at the lower end of the tray 14 and the magnet 17 on the tray carrier 13 attract each other, thereby achieving the positioning of the tray 14.

[0026] The outer shell component 1 is provided with a tray 14 outlet corresponding to the tray 14, and a door plate 2 is provided outside the tray 14 outlet, and the upper side of the door plate 2 is hinged to the outer shell component 1. The door plate 2 can be flipped up or down, and under normal circumstances, the door plate 2 automatically closes the tray 14 outlet under the action of gravity.

[0027] In the utility model, a tray 14 is arranged below the moving area of ​​the orifice plate seat 11, and the tray 14 is used to catch the dripping reagent to prevent the reagent from dripping into the inside of the equipment and causing pollution to the equipment; when it is necessary to take out the tray 14, one end of the tray 14 is pushed by the pushing device, and after the tray 14 is pushed by the pushing device, the other end of the tray 14 will push open the compartment door plate 2 and extend from the tray 14 outlet, and the tray 14 automatically pushes the compartment door plate 2 open, which makes it convenient for the operator to take out the tray 14, and the pushing device pushes the tray 14 out and then resets it; when it is necessary to load the tray 14 into the equipment, it is only necessary to open the compartment door plate 2 first, and then load the tray 14 into the equipment from the tray 14 outlet, and place the tray 14 on the tray carrier 13; in the above manner, the removal and loading of the tray 14 are facilitated.

[0028] The present utility model is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of products under the inspiration of the present utility model. However, no matter what changes are made in the shape or structure, all those having the same or similar technical solutions as the present application fall within the protection scope of the present utility model.

Claims

1. A fully automatic plasmid purification instrument, characterized in that: It comprises a shell component, in which a first lifting device, a horizontal moving device and a tray are arranged, the first lifting device is connected to a pipette, the horizontal moving device is provided with a well plate seat for placing the well plate, and the horizontal moving device drives the well plate seat to move under the pipette; The pallet is placed on the pallet carrier, and the pallet is located below the moving area of ​​the orifice plate seat; a pushing device for pushing the pallet is provided at one end of the orifice plate seat, a pallet outlet corresponding to the pallet is provided on the outer shell component, a door plate is provided on the outer side of the pallet outlet, and the upper side of the door plate is hinged to the outer shell component.

2. A fully automatic plasmid purification instrument according to claim 1, characterized in that: The pushing device is an electric cylinder.

3. A fully automatic plasmid purification instrument according to claim 2, characterized in that: The electric cylinder is provided with a telescopic push rod, and the front end of the push rod is provided with a rubber head.

4. A fully automatic plasmid purification instrument according to claim 1, characterized in that: The pushing device is arranged on a fixing frame.

5. A fully automatic plasmid purification instrument according to claim 1, characterized in that: The tray carrier is provided with a guide groove area, the tray is placed in the guide groove area on the tray carrier, and the tray can move along the length direction of the guide groove area.

6. A fully automatic plasmid purification instrument according to any one of claims 1 to 5, characterized in that: The tray carrier is provided with a magnet, and the lower end of the tray is provided with a magnetic attraction piece corresponding to the magnet.

7. A fully automatic plasmid purification instrument according to claim 6, characterized in that: The magnetic attraction piece is made of iron.

8. A fully automatic plasmid purification instrument according to any one of claims 1 to 5, characterized in that: A second lifting device is provided in the shell component, and a magnetic sleeve fixing frame and a magnetic rod fixing frame are connected to the second lifting device. A plurality of magnetic sleeves are provided at the lower end of the magnetic sleeve fixing frame, and a plurality of magnetic rods are provided at the lower end of the magnetic rod fixing frame. The magnetic rods are correspondingly arranged above the magnetic sleeves; a cavity is provided on the magnetic sleeve for the magnetic rods to extend into.

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