Full-automatic nucleic acid extraction device

Abstract

The utility model provides a full-automatic nucleic acid extraction device which comprises a full-automatic nucleic acid extraction device body, a consumable storage assembly is installed on the front side of the full-automatic nucleic acid extraction device body, a clamping assembly is installed on the upper left side of the full-automatic nucleic acid extraction device body, and the clamping assembly is installed on the upper left side of the consumable storage assembly. Compared with the prior art, the full-automatic nucleic acid extraction device has the following beneficial effects that by arranging the full-automatic nucleic acid extraction device, a user only needs to place consumables and samples, the device can automatically complete nucleic acid extraction, the samples can respond at any time without waiting, the reaction is quick, the whole equipment is low in flux, and the whole equipment can be started at any time without accumulating the samples to a certain quantity; the starting-up cost is low, so that the waiting time can be reduced, and the influence on the detection accuracy due to long waiting time is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a full-automatic nucleic acid extraction device. Background Art

[0002] With the application of PCR technology in various fields, the application scenarios of supporting equipment such as nucleic acid extraction devices are also very broad. However, at present, first, the startup cost of large-throughput nucleic acid extraction devices is high, and they generally cannot be operated at any time. Instead, they are operated after a certain number of samples have accumulated. Therefore, the samples cannot be processed in time, which affects the accuracy of the results. Second, large-throughput samples are subjected to nucleic acid extraction at the same time, and the risk of cross-contamination between samples is high, especially when different samples are purified for the same target substance, such as when positive patient samples and negative patient samples are subjected to nucleic acid extraction at the same time, cross-contamination is prone to occur. Third, large-throughput nucleic acid extraction devices are expensive, and most of the using institutions often share one. Different samples need to be sent to a special nucleic acid extraction institution, which involves transportation costs and time delays, and there is a high risk of sample loss or accurate analysis of the results. Therefore, small-throughput, automated, low cross-contamination rate, fast reaction and ready-to-run nucleic acid extraction devices have become the bottleneck of the application of PCR technology in the field of diagnostic analysis. Therefore, we hope to design a nucleic acid extraction device with a new structure to solve this problem. Utility Model Content

[0003] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a fully automatic nucleic acid extraction device to solve the problems raised in the above-mentioned background technology.

[0004] The utility model is realized by the following technical scheme: a fully automatic nucleic acid extraction device, comprising: a fully automatic nucleic acid extraction device, a consumable storage component is installed on the front side of the fully automatic nucleic acid extraction device, a clamping component is installed on the upper left side of the fully automatic nucleic acid extraction device, and the clamping component is installed on the upper left side of the consumable storage component;

[0005] A Y-axis moving component is installed on the middle upper side of the fully automatic nucleic acid extraction device, a magnetic adsorption component is installed on the lower side of the front surface of the Y-axis moving component, an X-axis moving component is installed on the front upper side of the Y-axis moving component, a rotating cover-opening clamp is installed between the upper left side of the Y-axis moving component and the left side of the X-axis moving component, and a purified nucleic acid collection component is installed on the right rear side of the fully automatic nucleic acid extraction device;

[0006] The purified nucleic acid collection component is placed at the rear side of the consumable storage component, a row of pipetting components is installed between the upper right side of the Y-axis moving component and the right side of the X-axis moving component, and a single row of pipetting components is installed at the front side of the X-axis moving component.

[0007] As a preferred embodiment, a magnetic adsorption motor is installed on the lower right front side of the magnetic adsorption component, and a magnet is installed on the front end of the magnetic adsorption motor. The setting of the magnetic adsorption component allows the magnetic beads to carry the target substance to the inner wall of the pipette tip by the magnet, which is helpful for subsequent work.

[0008] As a preferred embodiment, a row lifting motor is installed at the lower end of the row pipetting assembly, a mounting plate is installed at the front side of the row pipetting assembly, and a piston motor is installed on the upper side of the mounting plate.

[0009] As a preferred embodiment, the lower end of the piston motor is equipped with multiple piston rods through a fixed block 1, and the lower end of each piston rod is movably installed inside the upper end of a piston lumen, and the upper sides of the multiple piston lumens are fixed inside the fixed block 2, and the left and right sides of the fixed block 2 are respectively fixedly connected to the fixed block 3 through a fixed rod;

[0010] The rear side of the fixed block three is fixedly connected to the upper end of the row lifting motor, the lower sides of the multiple piston tube cavities are fixedly connected to the fixed block three, the rear side of the fixed block two is connected to the front lower side of the mounting plate, and each lower end of the piston tube cavity is movably connected to a pipette tip one.

[0011] As a preferred embodiment, a sampling tube is placed on the left side of the consumable storage assembly, and a nucleic acid extraction well plate is placed on the right rear side of the consumable storage assembly. The nucleic acid extraction well plate contains magnetic beads for nucleic acid extraction. The consumable storage assembly can store a variety of consumables to facilitate fully automatic nucleic acid extraction.

[0012] As a preferred embodiment, a second pipette tip is placed on the front side of the consumable storage component, a third pipette tip is placed on the front side of the consumable storage component, and the third pipette tip is placed on the front side of the second pipette tip.

[0013] After adopting the above technical scheme, the beneficial effects of the utility model are as follows: by setting up a fully automatic nucleic acid extraction device, the user only needs to put consumables and samples, and the device automatically completes the nucleic acid extraction. The sample does not need to wait for the device to respond at any time, the response is fast, and the entire device has a low throughput. It is not necessary to accumulate samples to a certain number before starting the machine, and it can be started at any time, and the startup cost is low, which can also reduce the waiting time and reduce the impact on the accuracy of the test due to the long waiting time;

[0014] By setting up a row of pipetting components and a single row of pipetting components, in conjunction with a Y-axis moving component, a magnetic adsorption component, an X-axis moving component and a rotating opening clamp, manual operations can be reduced, transportation costs can be reduced, the impact of time can be reduced, and the accuracy of sample detection can be improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0016] Figure 1 The utility model is a schematic diagram of the overall structure of a fully automatic nucleic acid extraction device.

[0017] Figure 2 The utility model is a schematic diagram of the explosion of a fully automatic nucleic acid extraction device.

[0018] Figure 3 This is a schematic diagram of a front view of a fully automatic nucleic acid extraction device of the present invention.

[0019] Figure 4 It is a schematic diagram of a magnetic adsorption component of a fully automatic nucleic acid extraction device of the utility model.

[0020] Figure 5 The utility model is a schematic diagram of a linked liquid transfer assembly of a fully automatic nucleic acid extraction device.

[0021] Figure 6 This is a schematic diagram of a consumable storage component of a fully automatic nucleic acid extraction device of the utility model.

[0022] In the figure, 1- fully automatic nucleic acid extraction device, 11- clamping assembly, 12- Y-axis moving assembly, 14- X-axis moving assembly, 15- rotating cover opening clamp, 17- single-row pipetting assembly, 18- purified nucleic acid collection assembly;

[0023] 13-magnetic adsorption component, 131-magnetic adsorption motor, 132-magnet;

[0024] 16-linked pipetting assembly, 161-linked lifting motor, 162-pipette tip 1, 163-piston lumen, 164-piston rod, 165-piston motor;

[0025] 19-consumable storage assembly, 191-sampling tube, 192-nucleic acid extraction well plate, 193-pipette tip 2, 194-pipette tip 3. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] See also Figures 1 to 6 The utility model provides a technical solution: a fully automatic nucleic acid extraction device 1, comprising: a fully automatic nucleic acid extraction device 1, a consumable storage component 19 is installed on the front side of the fully automatic nucleic acid extraction device 1, a clamping component 11 is installed on the upper left side of the fully automatic nucleic acid extraction device 1, and the clamping component 11 is installed on the upper left side of the consumable storage component 19;

[0028] A Y-axis moving component 12 is installed on the middle upper side of the fully automatic nucleic acid extraction device 1, a magnetic adsorption component 13 is installed on the lower side of the front surface of the Y-axis moving component 12, an X-axis moving component 14 is installed on the front upper side of the Y-axis moving component 12, a rotating cover opening clamp 15 is installed between the upper left side of the Y-axis moving component 12 and the left side of the X-axis moving component 14, and a purified nucleic acid collection component 18 is installed on the right rear side of the fully automatic nucleic acid extraction device 1;

[0029] The purified nucleic acid collection component 18 is placed at the rear of the consumable storage component 19 , a row of pipetting components 16 is installed between the upper right side of the Y-axis moving component 12 and the right side of the X-axis moving component 14 , and a single row of pipetting components 17 is installed at the front side of the X-axis moving component 14 .

[0030] Please refer to the figure, a magnetic adsorption motor 131 is installed on the lower right front side of the magnetic adsorption component 13, and a magnet 132 is installed at the front end of the magnetic adsorption motor 131. The setting of the magnetic adsorption component 13 allows the magnetic beads to carry the target substance to the inner wall of the pipette tip 162 by the magnet 132, which is helpful for subsequent work.

[0031] A row lifting motor 161 is installed at the lower end of the row pipetting assembly 16, a mounting plate is installed at the front side of the row pipetting assembly 16, and a piston motor 165 is installed on the upper side of the mounting plate.

[0032] The lower end of the piston motor 165 is equipped with a plurality of piston rods 164 through a fixed block 1, and the lower end of each piston rod 164 is movably installed inside the upper end of a piston tube cavity, and the upper sides of the plurality of piston tube cavities are fixed inside the fixed block 2, and the left and right sides of the fixed block 2 are respectively fixedly connected to the fixed block 3 through a fixed rod;

[0033] The rear side of fixed block three is fixedly connected to the upper end of the row lifting motor 161, the lower sides of multiple piston cavities are fixedly connected to fixed block three, the rear side of fixed block two is connected to the front lower side of the mounting plate, and each piston cavity lower end is movably connected to a pipette tip 162.

[0034] As the first embodiment of the present utility model, the following is the working process of the fully automatic nucleic acid extraction device 1:

[0035] Step 1: Connect the power cord and turn on the power switch. Each device will automatically reset and communicate normally.

[0036] Step 2: Place the sampling tube 191 containing sample information, the nucleic acid extraction consumables nucleic acid extraction well plate 192, the second pipette tip 193, and the third pipette tip 194 into the consumable storage component 19, and place the opened collection and purification nucleic acid container into the purified nucleic acid collection component 18;

[0037] Step 3: Check that everything is correct and start the program. The Y-axis moving assembly 12 and the X-axis moving assembly 14 move the rotating cover opening clamp 15 to the top of the first sampling tube 191 in a coordinated manner. The rotating cover opening clamp 15 descends to clamp the tube cover of the sampling tube 191 and moves the sampling tube 191 to the top of the clamping assembly 11 and then descends. When the sampling tube 191 is in place, the clamping assembly 11 clamps it immediately. At this time, the rotating cover opening clamp 15 rotates and rises until the tube cover and the sampling tube 191 are completely separated and the cover opening action is completed. The rotating cover opening clamp 15 returns to its original position with the tube cover;

[0038] Step 4, the rotating opening jaw 15 is reset with the tube cover, the Y-axis moving assembly 12 and the X-axis moving assembly 14 are linked to move the single-row pipette assembly 17 to the first row, the first pipette tip 1 162 is directly above (all the pipette tips 1, 2, and 3 in the full text are the same object), the lifting motor of the single-row pipette assembly 17 descends to obtain a pipette tip 3 and then resets; under the cooperation of the Y-axis moving assembly 12 and the X-axis moving assembly 14, it moves to the sampling tube 191 whose tube cover is opened in the third step, and descends to a suitable position through the lifting motor provided, and absorbs the sample in the first sampling tube 191 under the action of the liquid aspiration motor provided; under the cooperation of the Y-axis moving assembly 12 and the X-axis moving assembly 14, it moves to the first position of the first row of the nucleic acid extraction well plate 192, then descends and discharges the sample into the well plate, and the used pipette tip 3 is unloaded to the original position and the single-row pipette assembly 17 is reset.

[0039] Step 5: After the single-row pipetting assembly 17 is reset, the cover-opening jaw 15 is rotated to move the tube cover and re-close the tube cover on the sampling tube 191 opened in the third step, clamping the tube cover, and the clamping assembly 11 is reset to release the tube cover, and the cover-opening jaw 15 is rotated to put the sample tube back to the sample tube position of the consumable storage assembly 19 and reset;

[0040] Step 6: Repeat the third, fourth and fifth steps to move other samples to other positions in the first row of the nucleic acid extraction well plate 192 in the consumable storage component 19 to complete the sampling of the samples.

[0041] Step 7, after the sampling is completed, the Y-axis moving component 12 moves the piston tube cavity of the linked pipette component 16 to the top of the second row of pipette tips three of the consumable storage component 19, and the linked lifting motor 161 descends to obtain a row of pipette tips three and then resets; the Y-axis moving component 12 moves the linked pipette component 16 with the pipette tips three 194 to the fourth row position of the nucleic acid extraction well plate 192 of the consumable storage component 19, and then the linked lifting motor 161 descends to extend the pipette tips three 194 below the liquid surface, and the piston motor 165 reciprocates to pull and push the piston rod 164 to cooperate with the piston tube cavity to the fourth row of nucleic acid extraction well plate 192 After the liquid is aspirated and mixed, and the liquid is completely aspirated, the magnetic adsorption motor 131 of the magnetic adsorption component 13 pushes the magnet 132 out to stick to the pipette tip three 194 that is full of liquid. At this time, the magnetic beads in the liquid are attracted to the inner wall of the pipette tip three 194 with the target substance by the magnet 132, and the piston motor 165 pushes the piston rod 164 downward to discharge all the liquid in the pipette tip three 194 into the fourth row of nucleic acid extraction well plates 192. The row lifting motor 161 carries the pipette tip three 194 with the magnetic beads on the inner wall up until it is a certain distance away from the nucleic acid extraction well plate 192, completing the sample lysis and the capture of the target substance.

[0042] Step 8: The Y-axis moving component 12 moves the pipette tip 194 with magnetic beads to the 5th row of the nucleic acid extraction well plate 192 of the consumable storage component 19, and the row lifting motor 161 lowers the pipette tip 194 with magnetic beads on the inner wall until it is completely immersed in the liquid surface of the 5th row of the nucleic acid extraction well plate 192. The magnetic adsorption motor 131 of the magnetic adsorption component 13 resets the magnet 132 to disengage the pipette tip 194 with magnetic beads on the inner wall, and the piston motor 165 reciprocates to pull and push the piston rod 164 to cooperate with the piston lumen to suck and discharge the liquid in the 5th row of the nucleic acid extraction well plate 192 to completely remove the magnetic beads on the inner wall. After washing and mixing, the liquid is completely sucked out, and the magnetic adsorption motor 131 of the magnetic adsorption component 13 pushes the magnet 132 to stick to the pipette tip three 194 full of liquid. At this time, the magnetic beads in the liquid are attracted to the inner wall of the pipette tip three 194 with the target substance by the magnet 132, and the piston motor 165 pushes the piston rod 164 to move downward to discharge all the liquid in the pipette tip three 194 into the 5th row of nucleic acid extraction well plates 192. The row lifting motor 161 carries the pipette tip three 194 with the magnetic beads on the inner wall up until it is a certain distance away from the nucleic acid extraction well plate 192, completing the first cleaning of the target substance.

[0043] Step 9, repeat step 8, move the pipette tip 3 194 containing the target substance to the 6th and 7th rows of the nucleic acid extraction well plate 192 of the consumable storage component 19, and complete the second and third cleaning of the target substance.

[0044] Step ten, the Y-axis moving component 12 moves the pipette tip three 194 with magnetic beads to the 8th row position of the nucleic acid extraction well plate 192 of the consumable storage component 19, the row lifting motor 161 brings the pipette tip three 194 with magnetic beads on the inner wall down until it is completely immersed in the liquid surface of the 8th row of the nucleic acid extraction well plate 192, the magnetic adsorption motor 131 of the magnetic adsorption component 13 resets the magnet 132 to separate the pipette tip three 194 with magnetic beads on the inner wall, the piston motor 165 reciprocates and pushes the piston rod 164 to cooperate with the piston lumen to suck and discharge the liquid in the 8th row of the nucleic acid extraction well plate 192 to completely wash out the magnetic beads on the inner wall, and the Y-axis moving component 12 cooperates with the row lifting motor 161 to move to return the used pipette tip three 194 to the 2nd row of the consumable storage component 19.

[0045] Step 11, the Y-axis moving component 12 moves the piston tube cavity of the linked pipetting component 16 to directly above the third row of pipette tips 193 of the consumable storage component 19, the linked lifting motor 161 descends to obtain a row of pipette tips 193 and then resets, the Y-axis moving component 12 moves the piston tube cavity with the pipette tips 193 to directly above the eighth row of nucleic acid extraction well plates 192, and the liquid in the eighth row of nucleic acid extraction well plates 192 is transferred to the pipette tips 193 through the piston motor 165 in cooperation with the piston rod 164 and the piston tube cavity, and the pipette tips 193 are discharged into the container of the purified nucleic acid collection component 18 through the Y-axis moving component 12 in cooperation with the linked lifting motor 161 and the piston motor 165 to complete the entire nucleic acid extraction process.

[0046] Step 12: Take out the container of the purified nucleic acid collection component 18, close the tube cover and put it into the PCR instrument for use.

[0047] See also Figures 2 to 6 A sampling tube 191 is placed on the left side of the consumable storage component 19, and a nucleic acid extraction well plate 192 is placed on the right rear side of the consumable storage component 19. The nucleic acid extraction well plate 192 contains magnetic beads for nucleic acid extraction. The consumable storage component 19 can store a variety of consumables to facilitate fully automatic nucleic acid extraction.

[0048] A second pipette tip 193 is placed on the front side of the consumable storage component 19 , a third pipette tip 194 is placed on the front side of the consumable storage component 19 , and the third pipette tip 194 is placed on the front side of the second pipette tip 193 .

[0049] As a second embodiment of the present utility model, based on the above-mentioned first embodiment, by setting up a fully automatic nucleic acid extraction device 1, the user only needs to put consumables and samples, and the device automatically completes the nucleic acid extraction. The sample does not need to wait for the device to respond at any time, the response is fast, and the entire device has a low throughput. It is not necessary to accumulate samples to a certain number before starting up, and it can be started at any time. The startup cost is low, which can also reduce the waiting time and reduce the impact on the accuracy of the test due to the long waiting time;

[0050] By setting up a row of pipetting components 16 and a single row of pipetting components 17, in conjunction with the Y-axis moving component 12, the magnetic adsorption component 13, the X-axis moving component 14 and the rotating cover opening clamp 15, manual operations can be reduced, transportation costs can be reduced, the impact of time can be reduced, and the accuracy of sample detection can be improved.

[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fully automatic nucleic acid extraction device, include: The fully automatic nucleic acid extraction device (1) is characterized in that a consumable storage component (19) is installed on the front side of the fully automatic nucleic acid extraction device (1), a clamping component (11) is installed on the upper left side of the fully automatic nucleic acid extraction device (1), and the clamping component (11) is installed on the upper left side of the consumable storage component (19); A Y-axis moving component (12) is installed on the middle upper side of the fully automatic nucleic acid extraction device (1), a magnetic adsorption component (13) is installed on the lower side of the front surface of the Y-axis moving component (12), an X-axis moving component (14) is installed on the front upper side of the Y-axis moving component (12), a rotating cover opening clamp (15) is installed between the upper left side of the Y-axis moving component (12) and the left side of the X-axis moving component (14), and a purified nucleic acid collection component (18) is installed on the right rear side of the fully automatic nucleic acid extraction device (1); The purified nucleic acid collection component (18) is placed at the rear side of the consumable storage component (19), a row of pipetting components (16) is installed between the upper right side of the Y-axis moving component (12) and the right side of the X-axis moving component (14), and a single row of pipetting components (17) is installed at the front side of the X-axis moving component (14).

2. A fully automatic nucleic acid extraction device as claimed in claim 1, Features: A magnetic attraction motor (131) is installed on the lower right front side of the magnetic attraction component (13), and a magnet (132) is installed on the front end of the magnetic attraction motor (131).

3. A fully automatic nucleic acid extraction device as claimed in claim 1, Features: A row of lifting motors (161) are installed at the lower end of the row of pipetting components (16), a mounting plate is installed at the front side of the row of pipetting components (16), and a piston motor (165) is installed on the upper side of the mounting plate.

4. A fully automatic nucleic acid extraction device as claimed in claim 3, Features: The lower end of the piston motor (165) is equipped with a plurality of piston rods (164) through a fixed block 1, and the lower end of each of the piston rods (164) is movably installed inside the upper end of a piston tube cavity (163), and the upper sides of the plurality of piston tube cavities (163) are fixed inside a fixed block 2, and the left side and the right side of the fixed block 2 are respectively fixedly connected to the fixed block 3 through a fixed rod; The rear side of the fixed block three is fixedly connected to the upper end of the row lifting motor (161), the lower sides of the multiple piston tube cavities (163) are fixedly connected to the fixed block three, the rear side of the fixed block two is connected to the front lower side of the mounting plate, and the lower end of each piston tube cavity (163) is movably connected to a pipette tip one (162).

5. A fully automatic nucleic acid extraction device as claimed in claim 1, Features: A sampling tube (191) is placed on the left side of the consumable storage component (19), and a nucleic acid extraction well plate (192) is placed on the right rear side of the consumable storage component (19). The nucleic acid extraction well plate (192) contains magnetic beads for nucleic acid extraction.

6. A fully automatic nucleic acid extraction device as claimed in claim 5, Features: A second pipette tip (193) is placed on the front side of the consumable storage component (19), a third pipette tip (194) is placed on the front side of the consumable storage component (19), and the third pipette tip (194) is placed on the front side of the second pipette tip (193).

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