Cell separation device
By using a sliding connection plate and moving part in the cell separation device, combining magnets and booster springs, the problem of limited speed of the electric push rod is solved, the efficiency of separation between cells and shrapnel is improved, and faster shrapnel recovery and cell collection are achieved.
Patent Information
- Application Number
- CN202420759130.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-12
- Publication Date
- 2024-12-13
- Estimated Expiration
- 2034-04-12
AI Technical Summary
In the prior art, due to the properties of the electric push rod itself, the pressure plate strength is difficult to be quickly revoked, which affects the shrapnel recovery speed and thus affects the cell separation effect.
The sliding connection plate and the moving part are adopted to realize the relative activity between the moving parts through the driving assembly, and the magnet and rotating rod are used to achieve rapid compression and recovery of the shrapnel, and the shrapnel reset speed is increased by combining the power spring and the buffer block.
The separation effect between cells and shrapnel is improved, the obstacles in the shrapnel recovery process are reduced, and the separation efficiency is achieved faster.
Smart Images
Figure CN222160193U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell culture equipment, in particular to a cell separation device. Background Art
[0002] At present, in the process of in vitro cell culture, the culture plate is usually placed in a sterile environment with suitable temperature, suitable pH and certain nutrition by using the adherent culture method, so that the cells can grow and reproduce normally on the culture plate, thereby effectively increasing the number of cells to be cultured. However, the problem usually faced by this culture method is that it is not convenient to separate the cells from the culture plate when collecting the cultured cells.
[0003] In response to the above problems, after searching, a Chinese utility model patent with publication number CN220432843U discloses a cell culture separation device, the core solution of which is to drive the pressure plate to move by an electric push rod, thereby applying pressure to the spring, so that the spring is in an elastic contraction state. When separating cells, the pressure state of the pressure plate is canceled by the electric push rod, so that the spring quickly recovers under the action of its own elastic force, thereby using inertia to shake off the cells. This patent can effectively achieve the separation between cells and springs, thereby helping to quickly collect the cultured cells. The inventor believes that there are still certain technical defects in its solution. Specifically: the speed of the electric push rod is limited during movement, and its active end is difficult to complete a stroke displacement in an instant, so the force applied by the pressure plate is difficult to be quickly withdrawn in a short time, which ultimately makes the recovery process of the spring easy to be obstructed, thereby affecting the reset speed, which is easy to affect the degree of cell detachment. Utility Model Content
[0004] The purpose of this section is to summarize some aspects of the embodiments of the utility model and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and utility model name of this application to avoid blurring the purpose of this section, specification abstract and utility model name, and such simplifications or omissions cannot be used to limit the scope of the utility model.
[0005] In view of the following technical problems in the prior art: In the above patents, due to the nature of the electric push rod itself, it is difficult to quickly withdraw the pressure of the pressure plate, thereby affecting the recovery speed of the spring, thereby affecting the separation of cells. To solve this technical problem, the utility model provides the following technical solutions:
[0006] A cell separation device, comprising an incubator, further comprising:
[0007] There are at least two connecting plates slidably disposed on the incubator;
[0008] A spring sheet connected between the two connecting plates;
[0009] There are at least two moving parts slidably disposed on the incubator and located in the middle area of the two connecting plates, and the moving parts are detachably connected to the connecting plates;
[0010] A driving assembly is provided, through which the two moving parts move relative to each other.
[0011] As a preferred technical solution for a cell separation device, magnets are fixedly disposed on the moving part and the connecting plate.
[0012] As a preferred technical solution of a cell separation device, the driving assembly includes a rotating rod rotatably disposed on the incubator, and the moving part is threadedly engaged with the rotating rod.
[0013] As a preferred technical solution for a cell separation device, the incubator is fixedly provided with a plurality of guide rods, and the connecting plate is provided with ear plates which are slidably mounted on the guide rods.
[0014] As a preferred technical solution of a cell separation device, a booster spring is sleeved on the guide rod, and an end of the booster spring contacts the ear plate.
[0015] As a preferred technical solution of a cell separation device, a bracket is fixedly connected to the incubator, and the end of the guide rod is connected to the bracket via a buffer block.
[0016] As a preferred technical solution of a cell separation device, the spring piece is detachably connected to the connecting plate.
[0017] As a preferred technical solution of a cell separation device, the end of the spring is bent in a hook shape, and a slot matching the shape of the end of the spring is constructed on the connecting plate for accommodating the end of the spring.
[0018] The cell separation device provided by the utility model has the beneficial effect that: through the cooperation between the moving part and the driving assembly, the present application can conveniently realize the mutual approach between the two moving parts, thereby realizing the elastic compression of the spring sheet, and when the connection between the moving part and the connecting plate is released, the connecting plate is not constrained by external force. Therefore, compared with the prior art, the obstruction to the spring sheet during elastic recovery can be reduced, so as to realize the rapid recovery of the shape of the spring sheet, thereby improving the separation effect between the cell and the spring sheet. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following briefly introduces the drawings required for the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:
[0020] Figure 1 It is a three-dimensional diagram of the utility model.
[0021] Figure 2 It is a top view of the partial structure shown in the utility model.
[0022] Figure 3 For the utility model Figure 2 Enlarged view of point A.
[0023] Figure 4 For the utility model Figure 2 Enlarged view of point B.
[0024] Figure 5 It is a schematic diagram of the disassembly between the connecting plate and the spring sheet in the present invention.
[0025] Figure numerals: 1, incubator; 2, connecting plate; 3, spring; 4, moving part; 5, driving assembly; 501, rotating rod; 6, magnet; 7, guide rod; 8, ear plate; 9, booster spring; 10, bracket; 11, buffer block; 12, slot. DETAILED DESCRIPTION
[0026] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.
[0027] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.
[0028] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.
[0029] Secondly, the present invention is described in detail with reference to the schematic diagram. When describing the embodiments of the present invention, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.
[0030] Reference Figure 1-3 One embodiment of the utility model provides a cell separation device, including a culture box 1 for providing a culture environment for the in vitro growth process of cells. The culture box 1 can also be equipped with equipment such as atomizing nozzles, water tanks, and electric heating lamps to maintain the required culture environment. This part of the equipment will not be described in detail in the following. In addition, the utility model also includes the following parts:
[0031] The connecting plates 2 are slidably arranged on the incubator 1, and the number of the connecting plates 2 is at least two. The two connecting plates 2 can be arranged side by side longitudinally or side by side transversely. Figure 1 The setting method given in the figure is horizontally distributed side by side, which is for reference;
[0032] A spring sheet 3 connected between the two connecting plates 2, wherein the spring sheets 3 are multiple and arranged side by side, and are used for culturing cells;
[0033] A moving part 4 slidably disposed on the incubator 1, wherein the sliding direction thereof is consistent with the sliding direction of the connecting plate 2, and the number of the moving parts 4 is at least two, so as to correspond to the connecting plates 2 and be located in the middle area of the two connecting plates 2, and the moving part 4 and the connecting plates 2 can be connected or disconnected;
[0034] The driving component 5, specifically, the two moving parts 4 can move closer to each other or move away from each other under the action of the driving component 5;
[0035] Based on the above, when the cultured cells are separated from the springs 3, the two moving parts 4 are moved to be close to the two connecting plates 2 respectively through the driving component 5, and the two moving parts 4 are kept connected to the two connecting plates 2 respectively, so that the two moving parts 4 are brought close to each other under the action of the driving component 5, thereby pulling the two connecting plates 2 close to each other to achieve pressure on the spring 3, so that the spring 3 is in a bent state. In this state, when the connection between any one of the moving parts 4 and the connecting plate 2 is released, or the connection between the two moving parts 4 and the two connecting plates 2 is released at the same time, the two connecting plates 2 will not be pulled by each other, so that the spring 3 is not bound by external force. Therefore, compared with the disclosed patents in the prior art, the utility model has a faster reset speed when realizing the elastic reset of the spring 3, thereby being able to better improve the separation effect between the cells and the spring 3.
[0036] Further, see Figure 2 , Figure 3 and Figure 5 Magnets 6 are fixedly provided on the moving part 4 and the connecting plate 2. When the two are close to and in contact, the two magnets 6 are attracted to each other, thereby realizing the connection between the moving part 4 and the connecting plate 2. Through the magnetic connection method, when the two need to be separated, the two moving parts 4 are kept close to each other, and finally the two magnets 6 break free and separate under the elastic force provided by the spring sheet 3, thereby realizing the separation process between the connecting plate 2 and the moving part 4 more conveniently.
[0037] Further, see Figure 1 The driving assembly 5 includes a rotating rod 501 rotatably arranged on the incubator 1, and the moving part 4 is threadedly matched with the rotating rod 501. The threaded matching directions between the two moving parts 4 and the rotating rod 501 are opposite, so that while keeping the rotating rod 501 rotating, the two moving parts 4 can be simply and quickly moved closer to or away from each other.
[0038] Further, see Figure 1 and Figure 2 The incubator 1 is fixedly provided with guide rods 7, the number of which is several and symmetrically distributed on both sides of the incubator 1. The connecting plate 2 is constructed with ear plates 8, the number of which corresponds to the guide rods 7. The ear plates 8 are slidably sleeved on the guide rods 7 to achieve stable sliding of the connecting plate 2 on the incubator 1.
[0039] Further, see Figure 1 and Figure 2 A booster spring 9 is also sleeved on the guide rod 7, and its two ends are in contact with the ear plate 8, thereby providing an extrusion force in a separation direction between the two connecting plates 2, so that the elastic force provided by the booster spring 9 can be further increased when the spring piece 3 is reset. Since the spring piece 3 is in a squeezed state for a long time, its elastic force is easy to decay. Through the effect of the booster spring 9, even if the elastic force of the spring piece 3 is weakened, the recovery process of the spring piece 3 can still be achieved with the help of the booster spring 9.
[0040] Further, see Figure 1 , Figure 2 and Figure 4The incubator 1 is fixedly connected with a bracket 10, and the end of the guide rod 7 is connected to the bracket 10 through a buffer block 11, so as to be fixed on the incubator 1. The buffer block 11 can be a rubber block or a wooden block, and its size is larger than the diameter of the guide rod 7. When the ear plate 8 drives the connecting plate 2 to reset under the action of elastic force, the ear plate 8 finally hits the buffer block 11, thereby avoiding the rigid contact between the ear plate 8 and the incubator 1, so as to protect the entire device.
[0041] Further, see Figure 5 The shrapnel 3 and the connecting plate 2 are detachably connected, so that the shrapnel 3 can be directly removed from the device when cells are transplanted onto the shrapnel 3. At the same time, the shrapnel 3 can also be removed from the device when cleaning and maintaining it, thereby further improving the practical effect of the device.
[0042] Further, see Figure 2 and Figure 5 Regarding the connection method between the spring piece 3 and the connecting plate 2, the end of the spring piece 3 is bent in a hook shape, and the connecting plate 2 is configured with a slot 12 adapted to the shape of the end, which is used to accommodate the end. Therefore, when connecting the spring piece 3 and the connecting plate 2, the end of the spring piece 3 can be inserted along the slot 12, and the same is true when taking it out. This structure makes it more convenient to disassemble and assemble the spring piece 3 and the connecting plate 2.
[0043] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.
[0044] It should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.
Claims
1. A cell separation device, comprising an incubator (1), characterized in that: Also includes: A connecting plate (2) slidably disposed on the incubator (1), the number of which is at least two; A spring sheet (3) connected between the two connecting plates (2); There are at least two moving parts (4) slidably disposed on the incubator (1) and located in the middle area of the two connecting plates (2), and the moving parts (4) and the connecting plates (2) are detachably connected; A driving assembly (5), wherein the two moving parts (4) move relative to each other via the driving assembly (5).
2. The cell separation device according to claim 1, characterized in that: Magnets (6) are fixedly arranged on the moving part (4) and the connecting plate (2).
3. The cell separation device according to claim 1, characterized in that: The driving assembly (5) comprises a rotating rod (501) rotatably arranged on the incubator (1), and the moving part (4) is threadably engaged with the rotating rod (501).
4. The cell separation device according to claim 1, characterized in that: The incubator (1) is fixedly provided with a plurality of guide rods (7), and the connecting plate (2) is provided with ear plates (8) which are slidably sleeved on the guide rods (7).
5. The cell separation device according to claim 4, characterized in that: The guide rod (7) is sleeved with a booster spring (9), the end of which contacts the ear plate (8).
6. The cell separation device according to claim 4, characterized in that: A support (10) is fixedly connected to the incubator (1), and the end of the guide rod (7) is connected to the support (10) via a buffer block (11).
7. The cell separation device according to claim 1, characterized in that: The spring sheet (3) and the connecting plate (2) are detachably connected.
8. The cell separation device according to claim 7, characterized in that: The end of the spring sheet (3) is in a hook-shaped bent structure, and the connecting plate (2) is provided with a slot (12) that matches the shape of the end of the spring sheet (3) and is used to accommodate the end of the spring sheet (3).