Perfusion device for cord blood mesenchymal stem cell culture

Abstract

The utility model relates to the technical field of medical research, in particular to a perfusion device for cord blood mesenchymal stem cell culture, which comprises a base, the upper end of the base is fixedly connected with a plurality of supports, the upper ends of the supports are fixedly connected with a support plate, the upper end of the support plate is fixedly connected with a nutrition box, and the outer side of the lower end of the nutrition box is fixedly communicated with a parallel communication device; the perfusion device for umbilical cord blood mesenchymal stem cell culture has the beneficial effects that the parallel communication device is arranged, so that nutrients are provided for different incubators by the same nutrition box, and meanwhile, wastes generated by the different incubators can be collected and treated by the same collection box, so that the operation steps during cell culture are reduced, and the operation efficiency is improved. And the use efficiency of the device is improved.

Description

Technical Field

[0001] The utility model relates to the field of medical research, in particular to a perfusion device for culturing umbilical cord blood mesenchymal stem cells. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. In cell culture, a perfusion device is required to provide the cultured cells with nutrients to maintain cell activity, and the waste liquid produced by cell metabolism must also be collected and treated.

[0003] A traditional perfusion device usually corresponds to a culture box and a waste collection box separately. When adding nutrients and collecting waste, each culture box needs to be processed accordingly, and the operation steps are relatively complicated. Therefore, the utility model proposes a perfusion device for culturing umbilical cord blood mesenchymal stem cells to solve the above problems. Utility Model Content

[0004] The purpose of the utility model is to provide a perfusion device for culturing umbilical cord blood mesenchymal stem cells to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a perfusion device for culturing umbilical cord blood mesenchymal stem cells, the perfusion device for culturing umbilical cord blood mesenchymal stem cells comprising: a base, a plurality of brackets are fixedly connected to the upper end of the base, a support plate is fixedly connected to the upper end of the bracket, a nutrient box is fixedly connected to the upper end of the support plate, and a parallel connecting device is fixedly connected to the outer side of the lower end of the nutrient box.

[0006] Preferably, the parallel connection device includes a transport pipe, the lower end of the transport pipe is fixedly connected to a pull-out incubator, the inner end of the pull-out incubator is fixedly connected to a connecting pipe, the connecting pipes converge to a converging pipe, and the lower end of the converging pipe is clamped with a waste liquid collection box.

[0007] Preferably, the transport pipes are fixedly connected to the outer end of the nutrient tank in a circular array, and a transport pump is fixedly connected to the outer side of the transport pipes.

[0008] Preferably, a plurality of transport pipe holes are provided in a circular array on the upper end of the support plate, and transport pipes are sleeved in the transport pipe holes.

[0009] Preferably, a pull-out block is fixedly connected to the outer side of the pull-out incubator.

[0010] Preferably, the pull-out incubator has a base at the lower end corresponding to the sliding support.

[0011] Compared with the prior art, the beneficial effects of the utility model are:

[0012] The perfusion device for culturing umbilical cord blood mesenchymal stem cells proposed by the utility model is provided with a parallel connecting device, so that different culture boxes are provided with nutrients by the same nutrient box, and waste materials generated by different culture boxes can be collected and processed by the same collection box, thereby reducing the operation steps during cell culture and improving the use efficiency of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the main structure of the utility model;

[0014] Figure 2 It is a schematic diagram of the bottom side of the structure of the utility model;

[0015] Figure 3 It is a schematic diagram of the internal structure connection of the utility model.

[0016] In the figure: 1 nutrient box, 2 support plate, 3 transport pipe, 4 transport pump, 5 transport pipe hole, 6 base, 7 bracket, 8 pull-out block, 9 pull-out culture box, 10 waste liquid collection box, 11 connecting pipe, 12 converging pipe. DETAILED DESCRIPTION

[0017] In order to make the purpose and technical solution of the utility model clearly and completely described, and the advantages more clearly understood, the embodiments of the utility model are further described in detail in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are part of the embodiments of the utility model, not all of the embodiments, and are only used to explain the embodiments of the utility model, and are not used to limit the embodiments of the utility model. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0018] Embodiment 1

[0019] See also Figure 1-Figure 3 The utility model provides a technical solution: a perfusion device for culturing umbilical cord blood mesenchymal stem cells, the perfusion device for culturing umbilical cord blood mesenchymal stem cells comprises: a base 6, a plurality of brackets 7 are fixedly connected to the upper end of the base 6, a support plate 2 is fixedly connected to the upper end of the bracket 7, a nutrient box 1 is fixedly connected to the upper end of the support plate 2, the nutrient box 1 is used to provide nutrients for the cultured cells, and a parallel connecting device is fixedly connected to the outer side of the lower end of the nutrient box 1.

[0020] Embodiment 2

[0021] On the basis of embodiment one, the parallel connecting device includes a transport pipe 3, the lower end of the transport pipe 3 is fixedly connected to a pull-out incubator 9, the inner end of the pull-out incubator 9 is fixedly connected to a connecting pipe 11, the connecting pipes 11 converge and connect to a converging pipe 12, the lower end of the converging pipe 12 is clamped with a waste liquid collection box 10, the transport pipe 3 is fixedly connected to the outer end of the nutrient box 1 in a circular array, the outer side of the transport pipe 3 is fixedly connected to a transport pump 4, the upper end of the support plate 2 is provided with a plurality of transport pipe holes 5 in a circular array, the transport pipe 3 is sleeved in the transport pipe hole 5, the transport pump 4 can transport nutrients in the nutrient box 1 to the pull-out incubator 9 respectively, and at the same time, the waste liquid generated by cells in different pull-out incubators 9 is collected in the converging pipe 12 through the connecting pipe 11, and finally collected in the waste liquid collection box 10.

[0022] Embodiment 3

[0023] On the basis of the second embodiment, a pull-out block 8 is fixedly connected to the outside of the pull-out incubator 9, and a base 6 is correspondingly supported by the sliding at the lower end of the pull-out incubator 9. The pull-out incubator 9 can detect the cells cultured inside by pulling.

[0024] In actual use, when cells are cultured in the pull-out incubator 9, a connecting pipe 11 is fixedly connected to the inner end of the pull-out incubator 9, and the connecting pipes 11 converge and connect to a converging pipe 12, and a waste liquid collection box 10 is clamped at the lower end of the converging pipe 12. The transport pipe 3 is fixedly connected to the outer end of the nutrient box 1 in a circular array, and a transport pump 4 is fixedly connected to the outer side of the transport pipe 3. A plurality of transport pipe holes 5 are opened in a circular array at the upper end of the support plate 2, and the transport pipe 3 is sleeved in the transport pipe hole 5. The transport pump 4 can transport nutrients in the nutrient box 1 to the pull-out incubator 9 respectively. At the same time, the waste liquid generated by the cells in different pull-out incubators 9 is collected in the converging pipe 12 through the connecting pipe 11, and finally collected in the waste liquid collection box 10.

[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A perfusion device for culturing umbilical cord blood mesenchymal stem cells, Features: The perfusion device for culturing umbilical cord blood mesenchymal stem cells comprises: A base (6), wherein a plurality of brackets (7) are fixedly connected to the upper end of the base (6), a support plate (2) is fixedly connected to the upper end of the bracket (7), a nutrient box (1) is fixedly connected to the upper end of the support plate (2), and a parallel connection device is fixedly connected to the outer side of the lower end of the nutrient box (1).

2. A perfusion device for culturing umbilical cord blood mesenchymal stem cells according to claim 1, Features: The parallel connection device comprises a transport pipe (3), the lower end of the transport pipe (3) is fixedly connected to a pull-out incubator (9), the inner end of the pull-out incubator (9) is fixedly connected to a connecting pipe (11), the connecting pipes (11) converge to connect to a converging pipe (12), and the lower end of the converging pipe (12) is clamped to a waste liquid collection box (10).

3. A perfusion device for culturing umbilical cord blood mesenchymal stem cells according to claim 2, Features: The transport pipes (3) are fixedly connected to the outer end of the nutrient tank (1) in a circular array, and a transport pump (4) is fixedly connected to the outer side of the transport pipe (3).

4. A perfusion device for culturing umbilical cord blood mesenchymal stem cells according to claim 1, Features: The upper end of the support plate (2) is provided with a plurality of transport pipe holes (5) in a circumferential array, and the transport pipes (3) are sleeved in the transport pipe holes (5).

5. A perfusion device for culturing umbilical cord blood mesenchymal stem cells according to claim 2, Features: A drawer block (8) is fixedly connected to the outside of the drawer-type incubator (9).

6. A perfusion device for culturing umbilical cord blood mesenchymal stem cells according to claim 2, Features: The lower end of the pull-out incubator (9) is supported by a base (6) corresponding to the sliding connection.

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