Embryo vitrification freezing vessel
By designing an embryo vitrified freezer dish with fixed loading rods, the problem of inconvenient operation of embryo loading rods in the existing technology is solved, and a more efficient embryo freezing process and a larger capacity freezing capacity are achieved, and laboratory work efficiency and safety are improved. sex.
Patent Information
- Application Number
- CN202420620071.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-28
- Publication Date
- 2024-12-17
- Estimated Expiration
- 2034-03-28
AI Technical Summary
In the existing vitrification and freezing technology, the lack of tools to fix embryo loading rods leads to operators need to hold the loading rods in their hands, affecting work efficiency and physical consumption.
An embryonic vitrification freezing dish is designed, including the body of the frozen dish, loading tank and freezing tank. The vitrification freezing loading rod is fixed through the notch, reducing manual operation, improving work efficiency, and providing the same focal plane operation under a microscope, simplifying the embryo Transfer process.
By fixing the load rod, the workload of the operator is reduced, the efficiency of embryo loading and freezing is improved, repeated focus is avoided, and more embryos can be frozen at one time, reducing the risk of bacterial contamination.
Smart Images
Figure CN222171067U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of vitrification freezing, in particular to an embryo vitrification freezing dish. Background Art
[0002] like Figure 1 In the prior art, the vitrification freezing carrier rod includes a handle 1, an inner rod 2, a carrier rod 3, and an embryo marking point 4. The handle 1 is connected to one end of the inner rod 2, and the other end of the inner rod 2 is connected to the carrier rod 3. The top of the carrier rod 3 is provided with an embryo marking point 4. In the prior art, during vitrification freezing, on the inverted cover of the petri dish, 1 drop of WS and ES droplets are sterilely distributed to transfer the embryo from the petri dish to the WS droplet, and slowly balance in the WS droplet for a few minutes; then the sample is carefully transferred from the WS droplet to the ES droplet with the culture medium, and during the ES balance time, 1 drop of VS droplet is sterilely distributed, and then the sample is sucked into the tip of the pipette, transferred to the VS droplet, and placed for a period of time. Then the embryo is sucked under the microscope, the petri dish is removed, the vitrification carrier rod is taken under the microscope, the embryo is loaded on the vitrification carrier rod, and it is placed in liquid nitrogen for rapid freezing, and the vitrification carrier rod is sealed. The vitrification carrier rod loaded with the embryo is placed in a cryotube or freezing sleeve filled with liquid nitrogen to complete the vitrification freezing of the embryo. Then repeat the vitrification of the next embryo. From the above prior art, it can be seen that the vitrification of embryos in the laboratory is carried out on an inverted cover of a petri dish, and there is no tool used in the relevant patent. The carrier rod needs to be held by the operator by hand and cannot be fixed well, which affects the work efficiency. Summary of the invention
[0003] The utility model aims to provide an embryo vitrification freezing dish to solve the problems raised in the above background technology.
[0004] The vitrification freezing dish comprises a freezing dish body, a loading slot is arranged at the front end of the bottom of the freezing dish body, a freezing slot is arranged at the rear end of the loading slot, and notches are arranged on the left and right sides of the freezing dish body corresponding to the positions of the loading slots, and the size of the notches is adapted to the size of the inner rod of the vitrification freezing carrier rod. After the inner rod is placed in the notch, the vitrification freezing carrier rod can be fixed to prevent shaking. When the vitrification freezing is performed, it is not necessary to hold it with hands to fix it, which saves physical strength and improves work efficiency.
[0005] Furthermore, the loading slot is in the shape of an elongated strip, and the left end of the loading slot is flush with the inner wall of the left side of the freezing dish body, and the right end of the loading slot is flush with the inner wall of the right side of the freezing dish body. When the vitrification freezing carrier rod is fixed in the notch, the position of the embryo marking point is on the same focal plane of the microscope as the freezing slot. When transferring the embryo from the freezing slot to the embryo marking point, it is only necessary to simply translate the embryo vitrification freezing dish.
[0006] Furthermore, the freezing tanks have at least three rows, one row of WS washing solution tanks for containing WS washing solution, one row of ES balancing solution tanks for containing ES balancing solution, and one row of VS vitrification solution tanks for containing VS vitrification solution.
[0007] Furthermore, the front end of the WS washing solution tank column is marked with WS, the front end of the ES balancing solution tank column is marked with ES, and the front end of the VS vitrification solution tank column is marked with VS.
[0008] Further, the loading slot includes a left loading slot and a right loading slot, and the left loading slot and the right loading slot are spaced apart.
[0009] Furthermore, the left loading slot and the right loading slot are spaced apart and staggered from each other, the notch on the left side of the freezing dish body corresponds to the left loading slot, and the notch on the right side of the freezing dish body corresponds to the right loading slot.
[0010] Further, the freezing tank includes a left freezing tank and a right freezing tank, the left freezing tank has at least three columns, one column for WS washing solution tanks for WS washing solution, one column for ES balancing solution tanks for ES balancing solution, and one column for VS vitrification solution tanks for VS vitrification solution, and the right freezing tank has at least three columns, one column for WS washing solution tanks for WS washing solution, one column for ES balancing solution tanks for ES balancing solution, and one column for VS vitrification solution tanks for VS vitrification solution. Further, the freezing tank can be set in multiple rows, preferably 10 rows, so that an embryo vitrification freezing dish can freeze more embryos than a culture dish.
[0011] Furthermore, the depth of the loading groove is the same as that of the freezing groove, or the depth of the loading groove is deeper than that of the freezing groove, and the difference between the depth of the loading groove and the freezing groove is the thickness of the top end of the carrier rod. In this way, when the carrier rod is fixed at the notch, the freezing groove and the upper surface of the front end of the carrier rod are in the same focal plane of the microscope or almost in the same focal plane. When loading embryos, there is no need to repeatedly focus, thereby improving work efficiency.
[0012] Furthermore, the loading slots are used to place vitrification freezing rods; the capacity of each freezing slot is not less than 50 μL.
[0013] Furthermore, a fixing buckle of the loading rod is arranged in the loading slot. The vitrified freezing dish is also provided with an upper cover to prevent bacterial contamination.
[0014] The vitrification method using this device is as follows:
[0015] S1: Without dripping liquid in the loading tank, fix the vitrification rod in the loading tank through the notch, and fix the handle of the vitrification rod in the notch;
[0016] S2: Drop 50-100 μL of WS washing solution into the WS washing solution tank, take out the culture dish containing embryos from the incubator, and select the embryos with the best quality for vitrification under the microscope, transfer the embryos from the culture dish to the WS washing solution tank with culture medium, and start the timer. The embryos should slowly balance in the WS washing solution tank for 1-3 minutes; during this period, drop 50-100 μL of ES balancing solution into the ES balancing solution tank;
[0017] S3: Carefully transfer the embryos from the WS droplet to the ES equilibration solution tank with culture medium and start the timer. The embryos should slowly equilibrate in the ES equilibration solution tank for 6-10 minutes. During this period, 50-100 μL VS vitrification solution is dropped into the VS vitrification solution tank.
[0018] S4: Pipette the embryo into the pipette tip and transfer it to the VS vitrification solution tank and leave it there for at least 30 seconds.
[0019] S5: Transfer the embryo to a vitrified carrier straw; then take the vitrified carrier straw and seal it in liquid nitrogen;
[0020] S6: The vitrified carrier is placed in a cryotube or freezing sleeve filled with liquid nitrogen to complete the freezing of the embryo and proceed to the freezing of the next embryo.
[0021] Compared with the prior art, the utility model has the following beneficial effects: when the vitrification freezing dish of the utility model is in use, the carrier rod is fixed by the notch, which reduces the workload and improves the work efficiency; when loading embryos, the VS vitrification solution tank and the upper surface of the top of the carrier rod are located on the same focal plane of the microscope, and the transfer of embryos does not require repeated focusing, thereby improving the work efficiency; at the same time, the vitrification freezing dish has a large number of freezing slots, and more embryos can be frozen in one batch. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is a schematic diagram of the structure of a vitrification freezing rod in the prior art;
[0023] Figure 2 This is a schematic diagram of the three-dimensional structure of the vitrified freezing dish in the first embodiment of the utility model;
[0024] Figure 3 for Figure 2 Schematic diagram of the structure of the vitrified freezing dish from top view;
[0025] Figure 4 It is a schematic diagram of the three-dimensional structure of the vitrified freezing dish in the second embodiment of the present utility model.
[0026] Figure 5 for Figure 4 Schematic diagram of the top view of the vitrification freezing dish.
[0027] Figure 6 for Figure 4 Schematic diagram of the upper cover structure of the vitrification freezing dish.
[0028] In the figure: 1. handle; 2. inner rod; 3. carrier rod; 4. embryo marking point; 10. freezing dish body; 20. loading slot; 21. left loading slot; 22. right loading slot; 30. freezing slot; 31. left freezing slot; 32. right freezing slot; 40. notch; 41. left side notch; 42. right side notch, upper cover 50. DETAILED DESCRIPTION
[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0030] Example 1
[0031] like Figure 2-3 The utility model discloses a vitrified freezing dish, comprising a freezing dish body 10, a loading slot 20 is provided at the bottom front end of the freezing dish body 10, a freezing slot 30 is provided at the rear end of the loading slot 20, and a notch 40 is provided on the left and right sides of the freezing dish body 10 corresponding to the position of the loading slot. The loading slot 20 is in a long strip shape, and the left end of the loading slot 20 is flush with the inner wall of the left side of the freezing dish body 10, and the right end of the loading slot 20 is flush with the inner wall of the right side of the freezing dish body 10. The freezing slot 30 has at least three rows, one row of WS washing solution slots for WS washing solution, one row of ES balancing solution slots for ES balancing solution, and one row of VS vitrification solution slots for VS vitrification solution. Preferably, the freezing tank 30 includes a left freezing tank 31 and a right freezing tank 32. The left freezing tank 31 has at least 9 columns and is divided into 3 groups. Three columns of tanks in each group are adjacent in sequence, one column of WS washing solution tanks for WS washing solution, one column of ES balancing solution tanks for ES balancing solution, and one column of VS vitrification solution tanks for VS vitrification solution; the right freezing tank has 9 columns and is divided into 3 groups. Three columns of tanks in each group are adjacent in sequence, one column of WS washing solution tanks for WS washing solution, one column of ES balancing solution tanks for ES balancing solution, and one column of VS vitrification solution tanks for VS vitrification solution. Further, for the convenience of distinguishing the WS washing solution tanks, ES balancing solution tanks, and VS vitrification solution tanks, the front end of the WS washing solution tank column is marked with WS, the front end of the ES balancing solution tank column is marked with ES, and the front end of the VS vitrification solution tank column is marked with VS. They can also be marked above each tank, or different colors can be set on the tanks.
[0032] Furthermore, the depth of the loading slot 20 is the same as that of the freezing slot 30. Thus, when operating under a microscope, after the embryos are sucked from the VS vitrification solution tank, the microscope does not need to be adjusted or only needs to be fine-tuned, and the vitrification freezing dish is translated to make the loading slot 20 at the best visual position, and then the embryos can be loaded onto the vitrification freezing carrier rod.
[0033] Furthermore, the loading slot 20 can hold the vitrification freezing carrier straw, and the capacity of each freezing slot 30 is not less than 50 μL. In order to better fix the carrier straw, a fixing buckle of the carrier straw is provided in the loading slot, and the fixing buckle and the notch together fix the carrier straw.
[0034] Embodiment 2:
[0035] like Figure 4-5 , further improved on the basis of Example 1.
[0036] The loading slot 20 includes a left loading slot 21 and a right loading slot 22, and the left loading slot 21 and the right loading slot 22 are separated in the middle. Further, the left loading slot 21 and the right loading slot 22 are separated in the middle and staggered from each other, the left side notch 41 of the freezing dish body 10 corresponds to the left loading slot 21, and the right side notch 42 of the freezing dish body 10 corresponds to the right loading slot 22. The freezing tank 30 includes a left freezing tank 31 and a right freezing tank 32. The left loading slot 21 and the right loading slot 22 are staggered from each other, which can make the structure more compact, and the vitrified freezing carrier rod can be purchased directly from the market without special customization, which is conducive to reducing costs. Further, the vitrified freezing dish is also provided with an upper cover 50, which can prevent or reduce the invasion of bacteria.
[0037] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention of the utility model.
Claims
1. An embryo vitrification freezing dish, comprising a freezing dish body, characterized in that: The front end of the bottom of the freezing dish body is provided with a loading slot, the rear end of the loading slot is provided with a freezing slot, and the left and right sides of the freezing dish body are provided with notches corresponding to the positions of the loading slots.
2. The vitrified freezing dish according to claim 1, characterized in that: The loading slot is in the shape of an elongated strip, and the left end of the loading slot is flush with the inner wall of the left side of the freezing dish body, and the right end of the loading slot is flush with the inner wall of the right side of the freezing dish body.
3. The vitrified freezing dish according to claim 1, characterized in that: The freezing tanks have at least three columns, one column for containing WS washing solution tanks, one column for containing ES balancing solution tanks, and one column for containing VS vitrification solution tanks.
4. The vitrified freezing dish according to claim 3, characterized in that: The front end of the WS washing solution tank column is marked with WS, the front end of the ES balancing solution tank column is marked with ES, and the front end of the VS vitrification solution tank column is marked with VS.
5. The vitrified freezing dish according to claim 1, characterized in that: The loading slots include a left loading slot and a right loading slot, and the left loading slot and the right loading slot are spaced apart in between.
6. The vitrified freezing dish according to claim 5, characterized in that: The left loading slot and the right loading slot are spaced apart and staggered from each other, the notch on the left side of the freezing dish body corresponds to the left loading slot, and the notch on the right side of the freezing dish body corresponds to the right loading slot.
7. The vitrified freezing dish according to claim 5, characterized in that: The freezing tank includes a left freezing tank and a right freezing tank. The left freezing tank has at least three columns, one column of WS washing solution tanks for WS washing solution, one column of ES balancing solution tanks for ES balancing solution, and one column of VS vitrification solution tanks for VS vitrification solution. The right freezing tank has at least three columns, one column of WS washing solution tanks for WS washing solution, one column of ES balancing solution tanks for ES balancing solution, and one column of VS vitrification solution tanks for VS vitrification solution.
8. The vitrified freezing dish according to claim 1, characterized in that: The depth of the loading groove is the same as that of the freezing groove or the depth of the loading groove is deeper than that of the freezing groove, and the difference between the depth of the loading groove and the freezing groove is the thickness of the top end of the carrier rod.
9. The vitrified freezing dish according to claim 1, characterized in that: The capacity of the loading tank is not less than 1 ml; the capacity of each freezing tank is not less than 50 μL.
10. The vitrified freezing dish according to claim 1, characterized in that: A fixing buckle of the loading rod is arranged in the loading slot, and the vitrified freezing dish is also provided with an upper cover.