Novel nucleic acid sampling device

Abstract

The utility model discloses a novel nucleic acid sampling device which comprises a cylindrical sampling tube comprising an upper tube and a lower tube, a sealing cover is arranged at an opening of the upper pipe, a port of the lower end of the upper pipe is not sealed, and internal threads are arranged at the port of the lower end and used for being in threaded connection with the lower pipe; an external thread is arranged at the port of the upper end of the lower pipe and is used for being in threaded connection with the upper pipe; the lower pipe is in a sealed state, and a sealing film is arranged at a port of the lower pipe; a swab head is arranged at one end of the swab rod, and the diameter of the joint of the swab rod and the swab head is smaller than that of the swab rod, so that the swab rod is convenient to break. By adopting the nucleic acid sampling device disclosed by the invention, the sealing cover does not need to be repeatedly opened and closed in the whole collection process and detection process, the operation is simple and convenient, the swab rod is easy to break, and the pollution caused in the collection and detection processes is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of sampling tubes, in particular to a novel nucleic acid sampling device. Background Art

[0002] Nucleic acid collection refers to the process of collecting and extracting nucleic acids (such as DNA or RNA) from biological samples in biological experiments or clinical diagnosis. The main purpose of nucleic acid collection is to obtain nucleic acid information in the sample for subsequent molecular biology experiments, genetic testing, disease diagnosis, genetic research and other fields. Nucleic acid collection usually involves the following steps: Sample collection: Collect samples from organisms, such as blood, saliva, tissues, cells, etc., to obtain nucleic acids. The method of sample collection will vary depending on the specific experimental purpose and sample type. Nucleic acid extraction: The collected samples are subjected to nucleic acid extraction, that is, nucleic acids are separated from cells or tissues. Nucleic acid quantification and quality testing: The extracted nucleic acids are quantified and quality tested to determine the concentration and purity of the nucleic acids. This can be done by photometry, gel electrophoresis, fluorescence quantification and other methods. Nucleic acid storage: The extracted nucleic acids can be stored for a short or long term for subsequent experiments or analysis. Appropriate nucleic acid storage conditions can ensure the stability and integrity of the nucleic acids.

[0003] The nucleic acid sampling device currently in use is an integrated sampling tube with a screw cap. The tube cap needs to be rotated before and after collection, and the collection swab stick needs to be inserted into the tube mouth to break at the breaking point. The tube cap should be rotated and tightened before being sent to the laboratory. The lid should be opened before testing and stacked in a certain order. Be careful not to contaminate each other. The lids should not pollute the environment as much as possible and the order should be correct. After the machine test is completed, the lids should be put back in order. The collection process and the testing process require the lid to be opened and closed twice, which is inconvenient to operate and multiple openings are prone to increase contamination. In addition, the collection swab stick is not easy to break. It usually requires two hands to hold the two ends of the swab stick to break it, which further increases the possibility of contamination. Summary of the invention

[0004] In order to solve the problems of the prior art, the purpose of the utility model is to provide a new type of nucleic acid sampling device, which cooperates with the upper and lower segmented sampling tube and the easy-to-break swab stick, and does not need to repeatedly open and close the cover during the collection process and the detection process. The operation is simple, the swab stick is easy to break, and the pollution is reduced. The specific contents are as follows:

[0005] A novel nucleic acid sampling device, comprising:

[0006] The sampling tube is cylindrical and includes an upper tube and a lower tube; a sealing cap is provided at the opening of the upper tube, the lower end port of the upper tube is not sealed, and an internal thread is provided at the lower end port for connecting with the thread of the lower tube; an external thread is provided at the upper end port of the lower tube for connecting with the thread of the upper tube; the lower tube is in a sealed state, and a sealing film is provided at the port;

[0007] The swab stick has a swab head at one end, and the diameter of the connection between the swab stick and the swab head is smaller than the diameter of the swab stick, so it is easy to break.

[0008] Furthermore, a plurality of fan-shaped flexible baffles fixedly connected to the edge of the cover are arranged in the cover, the edges of the flexible baffles are adjacent to each other, and a hole is opened at the center of the circular surface formed by the plurality of flexible baffles.

[0009] Furthermore, the plane where the circular surface formed by the plurality of flexible baffles is located is lower than the plane where the end of the cover is located in the vertical direction.

[0010] Furthermore, the middle section of the upper tube is also provided with a breaking surface, which is parallel to the plane where the end surface of the upper tube is located, and a small hole that can pass the swab head is opened in the middle to assist in breaking the swab head.

[0011] Furthermore, the edge of the small hole opened in the middle of the broken surface is serrated; the distance between the broken surface and the bottom end of the upper tube is greater than the length of the swab head.

[0012] Furthermore, a nucleic acid preservation solution is sealed in the lower tube.

[0013] Furthermore, the outer surface of the cover is provided with concave-convex anti-slip textures.

[0014] The working principle of a new nucleic acid sampling device is as follows:

[0015] The sampling tube is an integrated type, divided into two parts, the upper and lower parts. The mouth of the upper tube 1 is sealed by a number of fan-shaped flexible baffles 31, with an open hole in the center, which is not airtight. After sampling, the swab head 61 can directly pass through the seal. A serrated inner edge fracture surface 11 is provided in the upper tube 1 of the sampling tube. The connection between the swab rod 6 and the swab head 61 is pressed against this place to break the swab head 61 so that the swab head 61 falls into the upper tube 1. The lower tube 2 and the upper tube 1 are separated into two physically independent containers by a thin mold material, which contain a nucleic acid preservation solution 5. When testing nucleic acid, the long gun tip of the pipette is used to vertically enter the tube mouth from the membrane cover 3 of the upper tube 1, pass through the fracture surface 11, pierce the mold material at the top of the lower tube 2, and hold the pipette to draw a circle, so that the long gun tip of the pipette expands the piercing range of the membrane material, so that the upper sampling head falls into the lower nucleic acid preservation solution 5, so that it is fully mixed and shaken before testing.

[0016] The utility model has the following beneficial effects compared with the prior art:

[0017] The use of the nucleic acid sampling device described in the present application eliminates the need to repeatedly open and close the cover during the entire collection and testing process, is easy to operate, and the swab stick is easy to break, thereby reducing contamination caused during the collection and testing process. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the three-dimensional structure of the sampling tube according to the embodiment of the utility model;

[0019] Figure 2 This is a schematic diagram of the structure of the swab stick according to an embodiment of the utility model;

[0020] Figure 3 This is a front cross-sectional view of the sampling tube according to the embodiment of the utility model;

[0021] Figure 4 This is an exploded view of the sampling tube structure described in the embodiment of the utility model;

[0022] Figure 5 This is a schematic diagram of the swab stick breaking according to an embodiment of the utility model;

[0023] Figure 6 Schematic diagram of the swab head stored in the upper tube according to the embodiment of the utility model

[0024] In the figure:

[0025] 1—upper tube, 11—fracture surface; 2—lower tube; 3—sealing cover, 31—flexible baffle; 4—sealing film; 5—nucleic acid preservation solution; 6—swab stick, 61—swab head. DETAILED DESCRIPTION

[0026] In order to make the technical means and creative features realized by the present invention and achieve the purpose of being easy to understand, the technical solution of the invention is further explained below in combination with one embodiment and a specific implementation method of a new nucleic acid sampling device provided by the present invention.

[0027] like Figure 1-6 As shown, the specific embodiments given for the invention are as follows:

[0028] A novel nucleic acid sampling device includes a sampling tube and a swab stick 6. The sampling tube is cylindrical, including an upper tube 1 and a lower tube 2; a cover 3 is provided at the opening of the upper tube 1, and a concave-convex anti-slip pattern is provided on the outer surface of the cover 3. Four fan-shaped flexible baffles 31 fixedly connected to the edge of the cover 3 are provided in the cover 3, the edges of the flexible baffles 31 are adjacent to each other, and a hole is provided at the center of the circular surface formed by the four flexible baffles 31. The plane where the circular surface formed by the four flexible baffles 31 is located is lower than the plane where the end of the cover 3 is located in the vertical direction. The lower end port of the upper tube 1 is not sealed, and an internal thread is provided at the lower end port for threaded connection with the lower tube 2; a breaking surface 11 is also provided and installed in the middle section of the upper tube 1, and the breaking surface 11 is parallel to the plane where the end face of the upper tube 1 is located, and a small hole that can pass through the swab head 61 is provided in the middle to assist in breaking the swab head 61. The edge of the small hole in the middle of the broken surface 11 is serrated; the distance between the broken surface 11 and the bottom end of the upper tube 1 is greater than the length of the swab head 61. The upper end of the lower tube 2 is provided with an external thread for threaded connection with the upper tube 1; the lower tube 2 is in a sealed state, and a sealing film 4 is provided at the port; the lower tube 2 is sealed with a nucleic acid preservation liquid 5. A swab head 61 is provided at one end of the swab rod 6, and the diameter of the connection between the swab rod 6 and the swab head 61 is smaller than the diameter of the swab rod 6, which is easy to break.

[0029] The sampling tube is an integrated type, divided into two parts, the upper and lower parts. The mouth of the upper tube 1 is sealed by four fan-shaped flexible baffles 31, with an open hole in the center, which is not airtight. After sampling, the swab head 61 can directly pass through the seal. A serrated inner edge fracture surface 11 is provided in the upper tube 1 of the sampling tube. The connection between the swab rod 6 and the swab head 61 is pressed against this place to break the swab head 61 so that the swab head 61 falls into the upper tube 1. The lower tube 2 and the upper tube 1 are separated into two physically independent containers by a thin mold material, which contain a nucleic acid preservation solution 5. When testing nucleic acid, the long gun tip of the pipette is used to vertically enter the tube mouth from the membrane cover 3 of the upper tube 1, pass through the fracture surface 11, pierce the mold material at the top of the lower tube 2, and hold the pipette to draw a circle, so that the long gun tip of the pipette expands the piercing range of the membrane material, so that the upper sampling head falls into the lower nucleic acid preservation solution 5, so that it is fully mixed and shaken before testing.

[0030] It should be understood that the above specific embodiments are only used to illustrate or explain the principles of the present invention, and do not constitute a limitation on the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc. made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. The claims attached to the present invention are intended to cover all changes and modifications that fall within the scope and boundaries of the attached claims, or the equivalent forms of such scope and boundaries.

Claims

1. A novel nucleic acid sampling device, characterized in that: include: The sampling tube is cylindrical and comprises an upper tube (1) and a lower tube (2); a sealing cap (3) is arranged at the opening of the upper tube (1); the lower end port of the upper tube (1) is not sealed, and an internal thread is arranged at the lower end port for being threadedly connected to the lower tube (2); an external thread is arranged at the upper end port of the lower tube (2) for being threadedly connected to the upper tube (1); the lower tube (2) is in a sealed state, and a sealing film (4) is arranged at the port; The swab rod (6) has a swab head (61) at one end, and the diameter of the connection between the swab rod (6) and the swab head (61) is smaller than the diameter of the swab rod (6), so as to facilitate breaking.

2. A novel nucleic acid sampling device as claimed in claim 1, characterized in that: A plurality of fan-shaped flexible baffles (31) fixedly connected to the edge of the cover (3) are arranged inside the cover (3), the edges of the flexible baffles (31) are adjacent to each other, and a hole is opened at the center of the circular surface formed by the plurality of flexible baffles (31).

3. A novel nucleic acid sampling device as claimed in claim 2, characterized in that: The plane where the circular surface formed by the plurality of flexible baffles (31) is located is lower than the plane where the end of the cover (3) is located in the vertical direction.

4. A novel nucleic acid sampling device as claimed in claim 1, characterized in that: The middle section of the upper tube (1) is also provided with a breaking surface (11), which is parallel to the plane where the end surface of the upper tube (1) is located, and a small hole is opened in the middle to allow the swab head (61) to pass through, so as to assist in breaking off the swab head (61).

5. A novel nucleic acid sampling device as claimed in claim 4, characterized in that: The edge of the small hole opened in the middle of the broken surface (11) is serrated; the distance between the broken surface (11) and the bottom end of the upper tube (1) is greater than the length of the swab head (61).

6. A novel nucleic acid sampling device as claimed in claim 1, characterized in that: The lower tube (2) is sealed with a nucleic acid preservation solution (5).

7. A novel nucleic acid sampling device according to claim 1 or 2, characterized in that: The outer surface of the sealing cover (3) is provided with concave and convex anti-slip patterns.

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