Hybrid reaction platform and nucleic acid chip detector

Abstract

The hybridization reaction platform comprises a first mounting base, a platform body, a heating device, a swinging device and a control device, the platform body is mounted on the first mounting base, and the two ends of the platform body are rotationally connected with the first mounting base, so that the two ends of the platform body can relatively swing up and down. The platform body has a reaction tank. The heating device is arranged at the bottom of the reaction tank and is used for heating the reaction tank. The swinging device comprises an eccentric wheel and a first driving part, the eccentric wheel and the platform body are connected to one end, connected with the first mounting base, of the platform body, and the driving part is electrically connected with the eccentric wheel and used for driving the eccentric wheel to do circular motion so as to drive the two ends of the platform body to swing up and down. The control device is electrically connected with the heating device and the first driving piece and used for controlling the working states of the heating device and the first driving piece. The swing structure of the hybridization reaction platform can fully mix hybridization reagents, is simple in structure and can lighten the hybridization reaction platform.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, and in particular to a hybridization reaction platform and a nucleic acid chip detector. Background Art

[0002] With the rapid development of molecular biology technology, analytical and diagnostic technology has been widely used in many fields such as clinical testing, medical research, disease prevention and control, and food safety and hygiene. Molecular hybridization instrument is a device that uses nucleic acid molecular hybridization technology to detect whether the genome to be tested contains known gene sequences. It is also an in vitro diagnostic device. Usually, one or more reagents need to be added to multiple reaction boxes, and hybridization reactions occur under heating or non-heating conditions. After the test measurement is completed, the waste liquid in the reaction tank is discharged.

[0003] Shaking is required at each stage of the hybridization reaction process. In the prior art, the hybridization reaction platform is usually shaken by moving forward and backward. However, since the reaction platform is relatively large and heavy, the shaking structure design is relatively complicated to achieve the purpose of sufficient mixing, which makes the entire hybridization reaction module relatively bulky. Therefore, a hybridization platform shaking structure with a simple structure is urgently needed. Utility Model Content

[0004] The utility model provides a hybridization reaction platform and a nucleic acid chip detector. The swing structure of the hybridization reaction platform can fully mix the hybridization reagent, and the swing structure has a simple structure, which can lighten the hybridization reaction platform.

[0005] According to the first aspect of the present application, in one embodiment, a hybridization reaction platform is provided, comprising:

[0006] a first mounting base;

[0007] The platform body is mounted on the first mounting base, and the two ends of the platform body are rotatably connected to the first mounting base so that the two ends of the platform body can swing up and down relatively; the platform body has a reaction groove, and the reaction groove is used for hybridization reaction;

[0008] A heating device, disposed at the bottom of the reaction tank, for heating the reaction tank;

[0009] The swing device comprises an eccentric wheel and a first driving member; the eccentric wheel is connected to the platform body at one end of the platform body connected to the first mounting base, and the first driving member is electrically connected to the eccentric wheel and is used to drive the eccentric wheel to make a circular motion, so as to drive the two ends of the platform body to swing up and down;

[0010] And a control device, which is electrically connected to the heating device and the first driving member and is used to control the working states of the heating device and the first driving member.

[0011] Further, the first mounting base includes a first mounting member and a second mounting member; the platform body includes a first end and a second end, the center of the first end is rotatably connected to the first mounting member, and the center of the second end is rotatably connected to the second mounting member;

[0012] The eccentric wheel is connected to the first end at a position offset from the center of the first end.

[0013] Furthermore, a reaction box is also included, the reaction box includes a plurality of quadruple hybridization boxes, the plurality of quadruple hybridization boxes are arranged at intervals in the reaction tank, and the quadruple hybridization box is used for hybridization reagents to perform hybridization reactions.

[0014] Furthermore, the quadruple hybridization box includes eight, and the eight quadruple hybridization boxes are arranged in two rows, and four quadruple hybridization boxes in each row are arranged from the first end to the second end. Further, it also includes a flip cover component, which is arranged on the platform body and is used to cover the reaction tank.

[0015] Furthermore, the flip cover assembly includes a cover member, a third mounting member, a driving wheel, a driven wheel, a transmission belt and a second driving member; the second driving member is electrically connected to the control device, and the control device is used to control the working state of the second driving member;

[0016] The third mounting member is mounted on the side of the reaction tank on the platform body, and one side of the covering member is rotatably mounted on the third mounting member;

[0017] The second driving member is installed on the platform body, the driving wheel is connected to the output end of the second driving member, the driven wheel is arranged on the connection end of the covering member and the third mounting member, and the driven wheel is connected to the driving wheel through a transmission belt; the second driving member is used to drive the driving wheel to drive the driven wheel to rotate to cover the reaction tank.

[0018] Furthermore, the flip cover assembly includes two, which are respectively arranged on both sides of the platform body.

[0019] Furthermore, the heating device includes a heating sheet, a graphite sheet, a heating sheet fixing member, a heat insulating sheet and a bottom plate. The graphite sheet, the heating sheet, the heating sheet fixing member, the heat insulating sheet and the bottom plate are stacked. The graphite sheet is the bottom of the reaction tank and is used for heat conduction.

[0020] Furthermore, the heating plate is a Pi heating plate; and the reaction tank is made of aluminum.

[0021] According to the second aspect of the present application, a nucleic acid chip detector is provided in one embodiment, and the nucleic acid chip detector includes the hybridization reaction platform protected by the first aspect.

[0022] The utility model provides a hybridization reaction platform and a nucleic acid chip detector, wherein the hybridization reaction platform comprises a first mounting base, a platform body, a heating device and a swinging device. By rotatably connecting the two ends of the platform body to the first mounting base, the function of the platform body swinging left and right can be realized, and by connecting the eccentric wheel and the end of the platform body connected to the first mounting base, when the first driving member drives the eccentric wheel to make a circular motion, the eccentric wheel can drive the platform body to move, so that the platform body swings left and right. The swinging structure of the hybridization platform is simple, and while achieving uniform mixing of the hybridization reagents in the reaction tank, it can also make the hybridization platform lightweight. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A schematic diagram of the structure of a hybridization reaction platform according to an embodiment of the utility model;

[0024] Figure 2 for Figure 1 An exploded view of the hybridization reaction platform;

[0025] Figure 3 is a schematic diagram of a portion of the structure of the hybridization reaction platform in the embodiment;

[0026] Figure 4 Schematic diagram of a partial structure of a hybridization reaction platform of an embodiment.

[0027] Figure numerals: hybridization reaction platform-100, first mounting base-110, first mounting member-111, second mounting member-112; platform body-120, reaction tank-121; heating device-130, graphite sheet-131, heating sheet-132, heating sheet fixing member-133, heat insulating sheet-134, bottom plate-135; rocking device-140, eccentric wheel-141, first driving member 142; flip cover assembly-150, covering member-151, third mounting member-152, driving wheel-153, driven wheel-154, transmission belt-155, second driving member-156; reaction box-160. DETAILED DESCRIPTION

[0028] The present application is further described in detail below by specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments adopt associated similar element numbers. In the following embodiments, many detailed descriptions are intended to enable the present application to be better understood. However, those skilled in the art can easily recognize that some of the features can be omitted in different situations, or can be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations based on the description in the specification and the general technical knowledge in the art.

[0029] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementations, and the operation steps involved in each embodiment can also be replaced or adjusted in a sequence in a manner that is obvious to those skilled in the art. Therefore, the specification and drawings are only for the purpose of clearly describing a certain embodiment and do not mean that the composition and / or sequence are necessary.

[0030] The serial numbers of the components in this document, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings).

[0031] The embodiment of the present utility model provides a hybridization reaction platform 100, please refer to Figure 1-4 , includes a first mounting base 110, a platform body 120, a heating device 130, a rocking device 140 and a control device (not shown).

[0032] Please refer to Figure 1-3 The platform body 120 is mounted on the first mounting base 110, and the two ends of the platform body 120 are rotatably connected to the first mounting base 110 so that the two ends of the platform body 120 can swing up and down relatively. The platform body 120 has a reaction tank 121, and the reaction tank 121 is used for hybridization reaction. The heating device 130 is arranged at the bottom of the reaction tank 121, and is used to heat the reaction tank 121. The swing device 140 includes an eccentric wheel 141 and a first driving member 142, the eccentric wheel 141 is connected to the platform body 120 at one end of the platform body 120 connected to the first mounting base 110, and the first driving member 142 is electrically connected to the eccentric wheel 141, and is used to drive the eccentric wheel 141 to do circular motion, so as to drive the two ends of the platform body 120 to swing up and down. The control device is electrically connected to the heating device 130 and the first driving member 142, and is used to control the working state of the heating device 130 and the first driving member 142.

[0033] In the present invention, both ends of the platform body 120 are rotatably connected to the first mounting base 110, so that the platform body 120 can swing left and right. In addition, the eccentric wheel 141 is connected to one end of the platform body 120 connected to the first mounting base 110, so that when the first driving member 142 drives the eccentric wheel 141 to make a circular motion, the eccentric wheel 141 can drive the platform body 120 to move, so that the two ends of the platform body 120 can swing up and down relatively. The swing device 140 of the present invention has a simple structure, and can achieve uniform mixing of the hybridization reagents while also making the hybridization platform lightweight.

[0034] In an embodiment of the present utility model, the first mounting base 110 includes a first mounting member 111 and a second mounting member 112, and the platform body 120 includes a first end and a second end, the center of the first end is rotatably connected to the first mounting member 111, and the center of the second end is rotatably connected to the second mounting member 112. The eccentric wheel 141 is connected to a position of the first end that deviates from the center of the first end. Specifically, the first driving member 142 is disposed on a side of the platform body 120 that is away from the reaction tank 121.

[0035] By suspending the platform body 120 on the first mounting base 110 and arranging the first driving member 142 on the side of the platform body 120 away from the reaction tank 121 , the swing function of the platform body 120 can be achieved while saving space.

[0036] Please refer to Figure 1 The hybridization reaction platform 100 of this embodiment further includes a reaction box 160, which includes a plurality of quadruple hybridization boxes, which are arranged at intervals in the reaction tank 121, and the quadruple hybridization boxes are used for hybridization reagents to perform hybridization reactions. Specifically, the quadruple hybridization boxes include eight, and the eight quadruple hybridization boxes are evenly arranged in two rows, and four quadruple hybridization boxes in each row are arranged in a direction from the first end to the second end.

[0037] In combination with actual experimental conditions, the utility model divides eight quadruple hybridization boxes into two rows, and the four quadruple hybridization boxes in each row are arranged in the direction from the first end to the second end of the platform body 120. In addition, since the first mounting member 111 and the second mounting member 112 are respectively arranged at the first end and the second end of the platform body 120, the maximum swing amplitude of the platform body 120 of ±5° can be achieved, which can ensure that the reagents in the quadruple hybridization box will not be shaken out and can also ensure the smooth progress of the experiment.

[0038] Please refer to Figure 3The hybridization reaction platform 100 further includes a flip cover assembly 150, which is disposed on the platform body 120 and is used to cover the reaction tank 121. Specifically, the flip cover assembly 150 includes a cover 151, a third mounting member 152, a driving wheel 153, a driven wheel 154, a transmission belt, and a second driving member 156. The second driving member 156 is electrically connected to a control device, and the control device is used to control the working state of the second driving member 156. The third mounting member 152 is mounted on the side of the reaction tank 121 on the platform body 120, and one side of the cover 151 is rotatably mounted on the third mounting member 152. The second driving member 156 is mounted on the platform body 120, the driving wheel 153 is connected to the output end of the second driving member 156, the driven wheel 154 is disposed on the connection end of the cover 151 and the third mounting member 152, and the driven wheel 154 is connected to the driving wheel 153 through a transmission belt 155. The second driving member 156 is used to drive the driving wheel 153 to drive the driven wheel 154 to rotate so as to cover the reaction tank 121. In addition, a silicone film is also provided on the side of the covering member 151 facing the reaction tank 121.

[0039] The utility model ingeniously utilizes the synchronous movement of the driving wheel 153 and the driven wheel 154 to connect one end of the covering member 151 with the driven wheel 154, so as to drive the covering member 151 to open or cover the notch of the reaction tank 121 through the second driving member 156, thereby avoiding the problem of mistakes caused by manual opening or closing of the cover and contamination of the hybridization reagent in the reaction tank 121.

[0040] Please refer to Figure 2 The hybridization reaction platform 100 includes two flip cover components 150 , which are respectively disposed on both sides of the platform body 120 .

[0041] In other embodiments, in addition to using the control device to control the working state of the heating device 130, a temperature control meter, a temperature probe and a relay may also be used. The temperature probe is electrically connected to the temperature control meter, and the temperature probe is used to monitor the temperature of the reaction tank 121. One end of the relay is electrically connected to the heating device 130, and the other end is electrically connected to the temperature control meter. The temperature control meter is electrically connected to the control device. The control device controls the working state of the temperature control meter, and the temperature control meter controls the opening and closing of the relay according to the temperature measured by the temperature probe, thereby controlling the heating temperature of the heating device 130.

[0042] Please refer to Figure 2 The heating device 130 includes a heating sheet 132, a graphite sheet 131, a heating sheet fixing member 133, a heat insulating sheet 134 and a bottom plate 135. The graphite sheet 131, the heating sheet 132, the heating sheet 132 fixing member, the heat insulating sheet 134 and the bottom plate 135 are stacked. The graphite sheet 131 is the bottom of the reaction tank 121 and is used for heat conduction. The heating sheet fixing member 133 is used to fix the heating sheet 132 and the graphite sheet 131 on the platform body 120.

[0043] Since the graphite sheet 131 has good heat conduction effect, the graphite sheet 131 is used as the bottom of the reaction tank 121, so that the temperature of each part of the reaction tank 121 can be evenly distributed.

[0044] In this embodiment, the heating plate 132 is a Pi heating plate 132, and the reaction tank 121 is made of aluminum. Since the reaction tank 121 is relatively large, each part in the reaction tank 121 needs to maintain a uniform temperature, so the heating plate 132 is selected as a Pi heating plate 132.

[0045] The hybridization reaction platform 100 in this embodiment can be applied to a 32-throughput nucleic acid chip detector using a membrane strip hybridization method, and can detect multiple genotypes.

[0046] The above specific examples are used to illustrate the present invention, which are only used to help understand the present invention and are not intended to limit the present invention. For those skilled in the art of the present invention, some simple deductions, deformations or substitutions can be made based on the idea of ​​the present invention.

Claims

1. A hybridization reaction platform, characterized in that: include: a first mounting base; The platform body is mounted on the first mounting base, and the two ends of the platform body are rotatably connected to the first mounting base so that the two ends of the platform body can swing up and down relatively; the platform body has a reaction groove, and the reaction groove is used for hybridization reaction; A heating device, disposed at the bottom of the reaction tank, for heating the reaction tank; The swing device comprises an eccentric wheel and a first driving member; the eccentric wheel is connected to the platform body at one end of the platform body connected to the first mounting base, and the first driving member is electrically connected to the eccentric wheel and is used to drive the eccentric wheel to make a circular motion, so as to drive the two ends of the platform body to swing up and down; and a control device, wherein the control device is electrically connected to the heating device and the first driving member and is used to control the working states of the heating device and the first driving member.

2. The hybridization reaction platform according to claim 1, characterized in that: The first mounting base includes a first mounting member and a second mounting member; the platform body includes a first end and a second end, the center of the first end is rotatably connected to the first mounting member, and the center of the second end is rotatably connected to the second mounting member; The eccentric wheel is connected to the first end at a position offset from the center of the first end.

3. The hybridization reaction platform according to claim 2, characterized in that: It also includes a reaction box, which includes a plurality of quadruple hybridization boxes, and the plurality of quadruple hybridization boxes are arranged in the reaction tank at intervals, and the quadruple hybridization boxes are used for hybridization reagents to perform hybridization reactions.

4. The hybridization reaction platform according to claim 3, characterized in that: The quadruple hybridization boxes include eight boxes, and the eight quadruple hybridization boxes are arranged in two rows, with four quadruple hybridization boxes in each row arranged in a direction from the first end to the second end.

5. The hybridization reaction platform according to claim 1, characterized in that: It also includes a flip cover assembly, which is arranged on the platform body and is used to cover the reaction tank.

6. The hybridization reaction platform according to claim 5, characterized in that: The flip cover assembly includes a cover member, a third mounting member, a driving wheel, a driven wheel, a transmission belt and a second driving member; the second driving member is electrically connected to the control device, and the control device is used to control the working state of the second driving member; The third mounting member is mounted on the side of the reaction tank on the platform body, and one side of the covering member is rotatably mounted on the third mounting member; The second driving member is installed on the platform body, the driving wheel is connected to the output end of the second driving member, the driven wheel is arranged on the connecting end of the covering member and the third mounting member, and the driven wheel is connected to the driving wheel through the transmission belt; the second driving member is used to drive the driving wheel to drive the driven wheel to rotate to cover the reaction tank.

7. The hybridization reaction platform according to claim 6, characterized in that: The flip cover assembly includes two, which are respectively arranged on both sides of the platform body.

8. The hybridization reaction platform according to claim 1, characterized in that: The heating device comprises a heating sheet, a graphite sheet, a heating sheet fixing part, a heat insulating sheet and a bottom plate, wherein the graphite sheet, the heating sheet, the heating sheet fixing part, the heat insulating sheet and the bottom plate are stacked, and the graphite sheet is the bottom of the reaction tank for heat conduction.

9. The hybridization reaction platform according to claim 8, characterized in that: The heating plate is a Pi heating plate; the reaction tank is made of aluminum.

10. A nucleic acid chip detector, characterized in that: The nucleic acid chip detector comprises the hybridization reaction platform as described in any one of claims 1-9.

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