High performance liquid chromatography column

Abstract

The utility model discloses a high performance liquid chromatographic column which comprises a column shell, positioning blocks are fixedly installed on the outer side of a top sealing cover and the outer side of a bottom sealing cover respectively, hoops are installed on the inner sides of the positioning blocks, plugs are installed on the inner sides of the top sealing cover and the bottom sealing cover in a sliding mode, springs are installed on the outer sides of sliding rods, and the springs are connected with the springs. The outer side of the sliding rod is connected with a supporting rod in a sliding mode, the inner side of the supporting rod is provided with filtering sieve plates, chromatographic packing is arranged between the filtering sieve plates, different filtering sieve plates and chromatographic packing need to be selected according to different analysis liquids, and urgent use and sudden stop of the chromatographic column can be achieved in a downward pressing mode; the upper positioning block and the lower positioning block can be rapidly clamped and fixed through the hoop, rapid disassembly of the device is achieved, the supporting rod and the plug can be disassembled by sliding the sliding block, the upper ring body and the lower ring body can be disassembled by screwing the screw, then the filtering sieve plate is replaced, and therefore the working efficiency can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of liquid chromatography columns, in particular to a high performance liquid chromatography column. Background Art

[0002] High performance liquid chromatography is an analytical technique widely used in chemical analysis, drug analysis, environmental monitoring and other fields. The high performance liquid chromatography column is a key component in the HPLC analysis system, and its performance directly affects the accuracy and repeatability of the analysis results.

[0003] The liquid chromatography column is a key component used in liquid chromatographs. It consists of a stationary phase and a mobile phase. The stationary phase is usually composed of an inner wall or porous ball coated with a thin layer or liquid, while the mobile phase is the substance that flows continuously in the chromatographic column. The function of the liquid chromatography column is to separate different components in a mixture so that they can be separated or mixed.

[0004] At present, commercial high-performance liquid chromatography columns mainly include silica-based columns, polymer columns, etc., but these columns have certain limitations. In chromatographic analysis, different filter screens and chromatographic fillers need to be selected according to the liquid to be analyzed. However, the existing liquid chromatography columns are complicated to disassemble and assemble. For example, the columns have poor stability and do not have an anti-dumping function.

[0005] Currently, no effective solution has been proposed for the problems in the related technologies. Utility Model Content

[0006] In view of the problems in the related technology, the utility model proposes a high performance liquid chromatography column to overcome the above technical problems existing in the existing related technology.

[0007] To this end, the specific technical solutions adopted by the utility model are as follows:

[0008] A high performance liquid chromatography column comprises a column shell, a top sealing cover is slidably mounted on the top end of the column shell, a bottom sealing cover is slidably mounted on the bottom end of the column shell, positioning blocks are fixedly mounted on the outer sides of the top sealing cover and the bottom sealing cover, a clamp is mounted on the inner side of the positioning block, a plug is slidably mounted on the inner side of the top sealing cover and the bottom sealing cover, a sliding rod is fixedly connected to the center of the plug, a spring is mounted on the outer side of the sliding rod, a support rod is slidably connected to the outer side of the sliding rod, a filter screen plate is mounted on the inner side of the support rod, chromatographic fillers are arranged between the filter screen plates, and the column shell comprises an upper part, an upper second part, a middle part, a lower second part and a lower part.

[0009] As a further solution of the utility model, the positioning blocks are arranged symmetrically in the upper and lower parts, and the clamps are provided in three groups, and the three groups of clamps are arranged at equal intervals.

[0010] As a further solution of the utility model, a slider is slidably installed on the inner end of the support rod, and a spring is installed between the slider and the support rod.

[0011] As a further solution of the utility model, the inner sides of the upper part and the lower part are both provided with a card slot, and the size of the upper part is equal to the size of the lower part.

[0012] As a further solution of the utility model, the inner sides of the upper two parts and the lower two parts are fixedly connected with wire sleeves, a lower ring body is installed on one side of the filter screen plate, and an upper ring body is installed on the other side of the filter screen plate, and screws are provided on the inner sides of the lower ring body and the upper ring body.

[0013] As a further solution of the utility model, the screw is spirally connected to the thread sleeve, and the filter screen plate is provided with two upper and lower groups.

[0014] As a further solution of the utility model, the column shell is in a hollow cylindrical shape, and the chromatographic filler is made of a composite of nano-silicon dioxide and polystyrene.

[0015] The beneficial effects of the utility model are:

[0016] The utility model is provided with a column shell, a top cover, a bottom cover, a plug, a spring, a support rod, an upper ring body, a lower ring body, and a filter screen plate. During chromatographic analysis, different filter screen plates and chromatographic fillers need to be selected according to different analyzed liquids. The chromatographic column can be used for emergency use and emergency stop by pressing down. The upper and lower positioning blocks can be quickly clamped and fixed by the clamp to realize the rapid disassembly of the device. The sliding slider can realize the disassembly of the support rod and the plug. The upper ring body and the lower ring body can be disassembled by screwing the screws, and then the filter screen plate can be replaced, thereby improving work efficiency.

[0017] The utility model is provided with a column shell, a filter screen plate, a chromatographic filler, etc. The filter screen plate has various types. According to the type of liquid analysis, one of polyethersulfone, nylon filter membrane and polytetrafluoroethylene filter membrane is selected and replaced. The chromatographic filler is made of nano-silicon dioxide and polystyrene composite, and the filling amount is 50-70%. It has high thermal stability. The nano-composite material has a high specific surface area, can provide more separation positions, improve the separation effect, and has a smooth surface that is easy to clean. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 It is a schematic diagram of the overall structure of a high performance liquid chromatography column according to an embodiment of the utility model;

[0020] Figure 2 It is a schematic diagram of the installation of a plug and an upper cover or a lower cover of a high performance liquid chromatography column according to an embodiment of the utility model;

[0021] Figure 3 It is a schematic diagram of the installation structure of a filter screen plate of a high performance liquid chromatography column according to an embodiment of the utility model;

[0022] Figure 4 It is a schematic diagram of the installation of a chromatographic filler of a high performance liquid chromatography column according to an embodiment of the utility model;

[0023] Figure 5 It is a schematic diagram of the overall structure of a support rod of a high performance liquid chromatography column according to an embodiment of the utility model;

[0024] Figure 6 The utility model is a schematic diagram of the installation structure of the bottom cover and the plug of a high performance liquid chromatography column according to an embodiment of the utility model.

[0025] In the figure:

[0026] 1. Column shell; 101. Upper part; 102. Upper second part; 103. Middle part; 104. Lower second part; 105. Lower part; 2. Top cover; 3. Bottom cover; 4. Positioning block; 5. Clamp; 6. Plug; 7. Slide rod; 8. Spring; 9. Support rod; 10. Slider; 11. Chromatographic packing; 12. Thread sleeve; 13. Lower ring body; 14. Filter screen plate; 15. Upper ring body; 16. Screw. DETAILED DESCRIPTION

[0027] To further illustrate each embodiment, the present invention provides drawings, which are part of the disclosure of the present invention and are mainly used to illustrate the embodiments. They can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, ordinary technicians in the field should be able to understand other possible implementation methods and advantages of the present invention. The components in the figures are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0028] According to an embodiment of the utility model, a high performance liquid chromatography column is provided.

[0029] Please refer to the instruction manual Figure 1-6According to an embodiment of the utility model, a high performance liquid chromatography column comprises a column shell 1, a top sealing cover 2 is slidably installed on the top of the column shell 1, a bottom sealing cover 3 is slidably installed on the bottom end of the column shell 1, positioning blocks 4 are fixedly installed on the outsides of the top sealing cover 2 and the bottom sealing cover 3, a clamp 5 is installed on the inside of the positioning block 4, a plug 6 is slidably installed on the inside of the top sealing cover 2 and the bottom sealing cover 3, a sliding rod 7 is fixedly connected to the center of the plug 6, a spring 8 is installed on the outside of the sliding rod 7, a support rod 9 is slidably connected to the outside of the sliding rod 7, a filter screen plate 14 is installed on the inside of the support rod 9, a chromatographic filler 11 is arranged between the filter screen plates 14, and the column shell 1 comprises an upper part 101, an upper second part 102, a middle part 103, a lower second part 104 and a lower part 105.

[0030] Adopting the anti-dumping method, when the liquid chromatography column is taken out, the plug 6 is tightly fitted with the top cover 2 or the bottom cover 3 under the action of the spring 8, which can prevent the liquid therein from leaking. By pressing down, the emergency stop of the chromatographic column can be realized. The upper and lower positioning blocks 4 can be quickly clamped and fixed by the clamp 5 to realize the rapid disassembly of the device. The sliding slider 10 can realize the disassembly of the support rod 9 and the plug 6. The upper ring body 15 and the lower ring body 13 can be disassembled by screwing the screw 16, and then the filter screen plate 14 can be replaced. The filter screen plate 14 has various types. Polyether sulfone: hydrophilic, low protein adsorption, suitable for rapid filtration of large volume aqueous solution samples. Mixed fiber filter membrane: not suitable for organic solvents, especially for water-based solvents. Nylon filter membrane: suitable for most organic solvents and aqueous solutions, strong acids can be used, not suitable for solvents such as DMF, THF, CHCI3. Polytetrafluoroethylene filter membrane: organic system, suitable for all organic solvents, acids and salts.

[0031] In one embodiment, please refer to the attached specification Figure 1-6 As a further solution of the utility model, the positioning blocks 4 are symmetrically arranged in the upper and lower parts, and the clamps 5 are provided in three groups, and the three groups of clamps 5 are arranged at equal intervals.

[0032] The top cover 2, the bottom cover 3 and the column shell 1 can be quickly assembled and disassembled by the clamp 5.

[0033] In another embodiment, please refer to the attached Figure 1-6 As a further solution of the utility model, a slider 10 is slidably installed on the inner end of the support rod 9, a spring is installed between the slider 10 and the support rod 9, and slots are provided on the inner sides of the upper part 101 and the lower part 105, and the size of the upper part 101 is equal to that of the lower part 105.

[0034] The sliding of the slider 10 can make the support rod 9 engage with or disengage from the column housing 1 .

[0035] In one embodiment, please refer to the attached specification Figure 1-6 As a further solution of the utility model, the inner sides of the upper two parts 102 and the lower two parts 104 are fixedly connected with a silk sleeve 12, a lower ring body 13 is installed on one side of the filter screen plate 14, and an upper ring body 15 is installed on the other side of the filter screen plate 14, and screws 16 are provided on the inner sides of the lower ring body 13 and the upper ring body 15, and the screws 16 are spirally connected to the silk sleeve 12, and the filter screen plate 14 is provided with two upper and lower groups.

[0036] The upper ring body 15 and the lower ring body 13 can be separated by screwing the screw 16 , and then the filter screen plate 14 can be replaced.

[0037] In another embodiment, please refer to the attached Figure 1-6 As a further solution of the utility model, the column shell 1 is in a hollow cylindrical shape, and the chromatographic filler 11 is made of a composite of nano-silicon dioxide and polystyrene.

[0038] The chromatographic filler is a new type of material, and its preparation method is: compounding a nano material with a high specific surface area with a polymer with good thermal stability to obtain a nano composite material with high stability and high separation efficiency.

[0039] Embodiment 1:

[0040] Column shell: made of polytetrafluoroethylene (PTFE) material, with an inner diameter of 2.1mm; chromatographic filler: composite of nano-silica (SiO2) and polystyrene (PS), with a filling amount of 50-70%; stationary phase: methanol is used as the mobile phase; mobile phase: water is used as the mobile phase; pump: a high-pressure pump is used, and the pump speed is 0.5-2.0mL / min; detector: an ultraviolet detector (UV Detector) is used, and the detection wavelength is 254nm.

[0041] Embodiment 2:

[0042] On the basis of Example 1, the chromatographic filler is changed to a composite of nano-silicon dioxide (SiO2) and polyvinyl pyrrolidone (PVP).

[0043] Embodiment 3:

[0044] Based on Example 1, the stationary phase was changed to acetonitrile (ACN) as the mobile phase.

[0045] The novel high performance liquid chromatography column has the following advantages: 1. Good stability: the novel chromatographic filler has high thermal stability and can be used for a long time under high temperature conditions without degradation; 2. Good separation effect: the nanocomposite material has a high specific surface area, can provide more separation positions, and improve the separation effect; 3. Long service life: the novel chromatographic filler has good mechanical strength and can withstand multiple chromatographic analyses without damage; 4. Easy to clean: the surface of the novel chromatographic filler is smooth, not easy to adsorb sample components, and is easy to clean and maintain. Adopting the anti-dumping method, when the liquid chromatography column is taken out, the plug 6 is tightly fitted with the top cover 2 or the bottom cover 3 under the action of the spring 8, which can prevent the liquid therein from leaking. By pressing down, the emergency stop of the chromatographic column can be realized. The upper and lower positioning blocks 4 can be quickly clamped and fixed by the clamp 5 to realize the rapid disassembly of the device. The sliding slider 10 can realize the disassembly of the support rod 9 and the plug 6. The upper ring body 15 and the lower ring body 13 can be disassembled by screwing the screw 16, and then the filter screen plate 14 can be replaced. The filter screen plate 14 has various types. Polyether sulfone: hydrophilic, low protein adsorption, suitable for rapid filtration of large volume aqueous solution samples. Mixed fiber filter membrane: not suitable for organic solvents, especially for water-based solvents. Nylon filter membrane: suitable for most organic solvents and aqueous solutions, strong acids can be used, not suitable for solvents such as DMF, THF, CHCI3. Polytetrafluoroethylene filter membrane: organic system, suitable for all organic solvents, acids and salts.

[0046] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A high performance liquid chromatography column, comprising a column shell (1), characterized in that: A top sealing cover (2) is slidably mounted on the top of the column shell (1), a bottom sealing cover (3) is slidably mounted on the bottom of the column shell (1), positioning blocks (4) are fixedly mounted on the outer sides of the top sealing cover (2) and the bottom sealing cover (3), a clamp (5) is mounted on the inner side of the positioning block (4), a plug (6) is slidably mounted on the inner side of the top sealing cover (2) and the bottom sealing cover (3), a sliding rod (7) is fixedly connected to the center of the plug (6), a spring (8) is mounted on the outer side of the sliding rod (7), a support rod (9) is slidably connected to the outer side of the sliding rod (7), a filter screen plate (14) is mounted on the inner side of the support rod (9), and a chromatographic filler (11) is arranged between the filter screen plates (14); The column shell (1) comprises an upper portion (101), upper second portions (102), a middle portion (103), lower second portions (104) and a lower portion (105).

2. A high performance liquid chromatography column according to claim 1, characterized in that: The positioning blocks (4) are arranged symmetrically in an upper and lower manner, and three groups of the clamps (5) are provided. The three groups of the clamps (5) are arranged at equal intervals.

3. A high performance liquid chromatography column according to claim 1, characterized in that: A slider (10) is slidably mounted on the inner end of the support rod (9), and a spring is mounted between the slider (10) and the support rod (9).

4. A high performance liquid chromatography column according to claim 1, characterized in that: The inner sides of the upper part (101) and the lower part (105) are both provided with a card slot, and the size of the upper part (101) is equal to the size of the lower part (105).

5. A high performance liquid chromatography column according to claim 1, characterized in that: The inner sides of the upper two parts (102) and the lower two parts (104) are fixedly connected with a thread sleeve (12); a lower ring body (13) is installed on one side of the filter screen plate (14); an upper ring body (15) is installed on the other side of the filter screen plate (14); and screws (16) are provided on the inner sides of the lower ring body (13) and the upper ring body (15).

6. A high performance liquid chromatography column according to claim 5, characterized in that: The screw (16) is spirally connected to the thread sleeve (12), and the filter screen plate (14) is provided with two upper and lower groups.

7. A high performance liquid chromatography column according to claim 1, characterized in that: The column shell (1) is in the shape of a hollow cylinder, and the chromatographic filler (11) is made of a composite of nano-silicon dioxide and polystyrene.

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