Rapid detection device for human cytomegalovirus

Abstract

The utility model discloses a human cytomegalovirus rapid detection device which comprises a reagent tube and a reagent tube cover used for sealing the reagent tube, and further comprises a material storage barrel arranged above the reagent tube cover, a crRNA-Cas12a enzyme complex is stored in the material storage barrel through a feeding assembly, the material storage barrel is made of a rubber material, and the material storage barrel is made of a plastic material. The reagent tube is filled with a buffer solution and a fluorescent probe, a through hole is formed in the reagent tube cover, the lower end of the storage barrel is detachably connected with the through hole through a threaded clamp, and a control assembly for controlling liquid adding is arranged in the through hole. According to the rapid detection device for the human cytomegalovirus, the reagent tube is matched with the crRNA-Cas12a enzyme complex on the reagent tube cover, the buffer solution and the fluorescent probe, so that the rapid detection operation on the human cytomegalovirus is completed, and the addition of the crRNA-Cas12a enzyme complex is sealed and controlled in the whole detection process; and the probability of pollution caused by contact with air in the entering process is reduced, and the detection result is ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of human cytomegalovirus detection, and specifically relates to a human cytomegalovirus rapid detection device. Background Art

[0002] For the determination of human cytomegalovirus, it is necessary to use detection equipment for auxiliary detection. The process of using the detection setting for auxiliary detection is time-consuming and costly, and is not convenient for the rapid detection of human cytomegalovirus. In addition, in the process of using the detection equipment for detection, the titration and addition process of the detection reagent is prone to contact and contamination with the air, which affects the detection results.

[0003] In view of this, the present utility model is proposed. Utility Model Content

[0004] In order to solve the above technical problems, the basic concept of the technical solution adopted by the utility model is:

[0005] A rapid detection device for human cytomegalovirus, comprising a reagent tube and a reagent tube cover for sealing the reagent tube, and also comprising a storage barrel arranged above the reagent tube cover, wherein the crRNA-Cas12a enzyme complex is stored in the storage barrel through a feeding component, and the storage barrel is made of rubber material, the interior of the reagent tube is filled with a buffer solution and a fluorescent probe, the reagent tube cover is provided with a through hole, the lower end of the storage barrel is detachably connected to the through hole through a threaded card, and a control component for liquid addition control is arranged inside the through hole;

[0006] A sealing component is arranged on the reagent tube cover and is used for sealing after the reagent tube cover is closed on the reagent tube.

[0007] The control component includes a fixed cylinder fixed inside the through hole, a conical groove is opened at the lower end of the fixed cylinder, a ball is arranged inside the through hole for abutting against the conical groove to block the fixed cylinder, and an elastic component is arranged inside the through hole for pushing the ball.

[0008] The elastic component includes a mounting plate fixed inside the through hole, the ball is located between the fixing tube and the mounting plate, an extrusion spring for pushing and extruding the ball is connected between the mounting plate and the ball, and the ball is abutted against the conical groove under the elastic force of the extrusion spring, and a guide component for guiding the movement of the ball is provided on the mounting plate.

[0009] The mounting plate is provided with a plurality of openings for liquid circulation.

[0010] The guide assembly comprises a sleeve fixed on the mounting plate, a slide rod is slidably connected to the sleeve, one end of the slide rod is fixed to the ball, and the extrusion spring is sleeved on the outer side of the sleeve.

[0011] The sealing assembly comprises a mounting groove opened on the outside of the reagent tube cover, and a sealing ring is installed on the mounting groove.

[0012] The feeding assembly comprises a feeding pipe which is installed in communication with the upper end of the material storage barrel, and a control valve for controlling the inlet and outlet of materials is installed on the feeding pipe.

[0013] Compared with the prior art, the utility model has the following beneficial effects:

[0014] The utility model provides a rapid detection device for human cytomegalovirus, which completes the rapid detection operation of human cytomegalovirus through the mutual cooperation of the crRNA-Cas12a enzyme complex, the buffer solution and the fluorescent probe on the reagent tube and the reagent tube cover. The addition of the crRNA-Cas12a enzyme complex is sealed and controlled during the entire detection process, thereby reducing the probability of contamination caused by contact with air during the introduction process and ensuring the detection result.

[0015] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In the attached picture:

[0017] Figure 1 It is a schematic diagram of the overall appearance structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the reagent tube cover structure of the utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the control component, elastic component and guide component of the utility model;

[0020] Figure 4 This is the detection principle diagram of the utility model.

[0021] In the figure: 101-reagent tube; 102-reagent tube cover; 2-storage cylinder; 3-through hole; 401-fixed cylinder; 402-conical groove; 403-sphere; 501-mounting plate; 502-extrusion spring; 6-opening; 701-sleeve; 702-slide rod; 801-mounting groove; 802-sealing ring; 901-feeding tube; 902-control valve. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. The following embodiments are used to illustrate the utility model.

[0023] like Figures 1 to 3As shown, a rapid detection device for human cytomegalovirus includes a reagent tube 101 and a reagent tube cover 102 for sealing the reagent tube 101, and also includes a storage barrel 2 arranged above the reagent tube cover 102, wherein the crRNA-Cas12a enzyme complex is stored in the storage barrel 2 through a feeding component, and the storage barrel 2 is made of rubber material;

[0024] The reagent tube 101 is filled with a buffer solution and a fluorescent probe, and a through hole 3 is provided on the reagent tube cover 102. The lower end of the storage barrel 2 is detachably connected to the through hole 3 via a threaded clamp, and a control component for liquid addition control is provided inside the through hole 3; a sealing component is provided on the reagent tube cover 102 for sealing after the reagent tube cover 102 is covered on the reagent tube 101;

[0025] The rapid detection operation of human cytomegalovirus is completed through the mutual cooperation of the crRNA-Cas12a enzyme complex, buffer and fluorescent probe on the reagent tube 101 and the reagent tube cover 102, and the addition of the crRNA-Cas12a enzyme complex is sealed and controlled during the entire detection process to reduce the probability of contamination caused by contact with air during the entry process, thereby ensuring the detection result.

[0026] The control component includes a fixed cylinder 401 fixed inside the through hole 3, a conical groove 402 is opened at the lower end of the fixed cylinder 401, a sphere 403 is arranged inside the through hole 3 for blocking the fixed cylinder 401 against the conical groove 402, and an elastic component is arranged inside the through hole 3 for pushing the sphere 403; the crRNA-Cas12a enzyme complex is flushed into the interior of the storage cylinder 2 through the feeding pipe 901, and after the flushing is completed, the feeding pipe 901 is blocked by the control valve 902, and the crRNA-Cas12a enzyme complex flushed into the interior of the storage cylinder 2 is flushed into the storage cylinder 2. The Cas12a enzyme complex is stored in the fixed cylinder 401 in a sealed state under the blocking effect of the sphere 403 and the conical groove 402 on the fixed cylinder 401. After the feeding is completed, the storage cylinder 2 is squeezed to increase the pressure inside the storage cylinder 2. Under the action of the internal pressure of the storage cylinder 2, the sphere 403 is pushed to no longer resist the conical groove 402. At this time, the crRNA-Cas12a enzyme complex inside the storage cylinder 2 flows into the interior of the reagent tube 101 through the gap between the conical groove 402 on the through hole 3 and the sphere 403.

[0027] The elastic component includes a mounting plate 501 fixed inside the through hole 3, the ball 403 is located between the fixed cylinder 401 and the mounting plate 501, and an extrusion spring 502 is connected between the mounting plate 501 and the ball 403 for pushing and squeezing the ball 403, and the ball 403 is pressed against the conical groove 402 under the elastic force of the extrusion spring 502, and a guide component for guiding the movement of the ball 403 is arranged on the mounting plate 501; the extrusion spring 502 on the mounting plate 501 facilitates pushing the ball 403 and the conical groove 402 to press against each other for sealing.

[0028] The mounting plate 501 is provided with a plurality of openings 6 for liquid circulation; the openings 6 facilitate the crRNA-Cas12a enzyme complex to pass through the mounting plate 501.

[0029] The guide assembly includes a sleeve 701 fixed on the mounting plate 501, a slide rod 702 is slidably connected to the sleeve 701, one end of the slide rod 702 is fixed to the ball 403, and the extrusion spring 502 is sleeved on the outside of the sleeve 701; the movement of the ball 403 after being subjected to force is guided by the sleeve 701 and the slide rod 702.

[0030] The sealing component includes an installation groove 801 opened on the outside of the reagent tube cover 102, and a sealing ring 802 is installed on the installation groove 801; the reagent tube cover 102 is covered on the reagent tube 101, and during the covering process, the sealing ring 802 on the installation groove 801 abuts against the inner wall of the reagent tube 101 for sealing.

[0031] The feeding assembly includes a feeding pipe 901 which is installed on the upper end of the storage barrel 2, and a control valve 902 for controlling the inlet and outlet of materials is installed on the feeding pipe 901; the feeding pipe 901 facilitates the feeding of materials into the interior of the storage barrel 2, and the control valve 902 facilitates the sealing after the feeding.

[0032] During the detection operation of human cytomegalovirus, the buffer solution and the fluorescent probe are pre-placed into the interior of the reagent tube 101, and the crRNA-Cas12a enzyme complex is flushed into the interior of the storage barrel 2 through the feeding tube 901. After the flushing is completed, the feeding tube 901 is blocked by the control valve 902, and the crRNA-Cas12a enzyme complex flushed into the interior of the storage barrel 2 is stored in the interior of the fixed barrel 401 in a sealed state under the blocking effect of the sphere 403 and the conical groove 402 on the fixed barrel 401;

[0033] After the buffer, fluorescent probe and crRNA-Cas12a enzyme complex are prepared, the reagent tube cover 102 is covered on the reagent tube 101. During the covering process, the sealing ring 802 on the mounting groove 801 abuts against the inner wall of the reagent tube 101 for sealing;

[0034] After the reagent tube cover 102 is closed, the sample to be tested, such as milk, is added to the reagent tube 101. After the addition is completed, the storage barrel 2 is squeezed to increase the pressure inside the storage barrel 2. Under the action of the internal pressure of the storage barrel 2, the sphere 403 is pushed to no longer resist the conical groove 402. At this time, the crRNA-Cas12a enzyme complex inside the storage barrel 2 flows into the interior of the reagent tube 101 through the gap between the conical groove 402 and the sphere 403 on the through hole 3, and is evenly mixed with the liquid inside the reagent tube 101. After sufficient reaction, the reagent tube 101 is irradiated with an ultraviolet lamp. If it is positive, it will emit fluorescence under the irradiation of the ultraviolet lamp. If not, no fluorescence will occur, thereby completing the rapid detection operation of human cytomegalovirus, and the entire detection process is sealed and controlled for the addition of the crRNA-Cas12a enzyme complex, reducing the probability of contamination caused by contact with air during the entry process, and ensuring the detection result;

[0035] The reasons for the detection reaction are as follows:

[0036] crRNA is a guide RNA gRNA that specifically recognizes the target sequence in CRISPR technology. It will specifically bind to the target sequence; crRNA will bind to the Cas12a enzyme to form a complex, and the Cas12a enzyme will specifically recognize a site PAM before the crRNA-bound fragment and cut it;

[0037] The fluorescent probe is specifically bound to the PAM site, which means that when the Cas12a enzyme cuts, the probe will also be cut off. The probe has two groups, FAM is the fluorescent group, and BHQ1 is the quenching group. When the two groups are connected together, they will not emit fluorescence. When they are cut and the two groups are separated, they will emit fluorescence.

Claims

1. A rapid detection device for human cytomegalovirus, comprising: A reagent tube (101) and a reagent tube cover (102) for sealing the reagent tube (101); It is characterized by further comprising: A material storage barrel (2) is arranged above the reagent tube cover (102), wherein the crRNA-Cas12a enzyme complex is stored in the material storage barrel (2) through a feeding component, and the material storage barrel (2) is made of a rubber material, the interior of the reagent tube (101) is filled with a buffer solution and a fluorescent probe, and the reagent tube cover (102) is provided with a through hole (3), the lower end of the material storage barrel (2) is detachably connected to the through hole (3) through a threaded clamp, and a control component for liquid addition control is arranged inside the through hole (3); A sealing component is arranged on the reagent tube cover (102) and is used for sealing the reagent tube cover (102) after it is covered on the reagent tube (101).

2. A rapid detection device for human cytomegalovirus according to claim 1, characterized in that: The control component comprises a fixed cylinder (401) fixed inside the through hole (3); a conical groove (402) is provided at the lower end of the fixed cylinder (401); a sphere (403) is provided inside the through hole (3) for abutting against the conical groove (402) to block the fixed cylinder (401); and an elastic component is provided inside the through hole (3) for pushing the sphere (403).

3. A rapid detection device for human cytomegalovirus according to claim 2, characterized in that: The elastic component comprises a mounting plate (501) fixed inside the through hole (3); the ball (403) is located between the fixing tube (401) and the mounting plate (501); a squeezing spring (502) for pushing and squeezing the ball (403) is connected between the mounting plate (501) and the ball (403); the ball (403) abuts against the conical groove (402) under the elastic force of the squeezing spring (502); and a guide component for guiding the movement of the ball (403) is arranged on the mounting plate (501).

4. A rapid detection device for human cytomegalovirus according to claim 3, characterized in that: The mounting plate (501) is provided with a plurality of openings (6) for liquid circulation.

5. A rapid detection device for human cytomegalovirus according to claim 3, characterized in that: The guide assembly comprises a sleeve (701) fixed on the mounting plate (501), a slide rod (702) is slidably connected to the sleeve (701), one end of the slide rod (702) is fixed to the ball (403), and the extrusion spring (502) is sleeved on the outside of the sleeve (701).

6. A rapid detection device for human cytomegalovirus according to claim 1, characterized in that: The sealing assembly comprises a mounting groove (801) opened on the outside of the reagent tube cover (102), and a sealing ring (802) is installed on the mounting groove (801).

7. A rapid detection device for human cytomegalovirus according to claim 1, characterized in that: The feeding assembly comprises a feeding pipe (901) which is installed on the upper end of the material storage barrel (2), and a control valve (902) for controlling the inlet and outlet of materials is installed on the feeding pipe (901).

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