Novel liquid-based adhesive glass slide

Abstract

The utility model relates to the technical field of glass slides, in particular to a novel liquid-based adhesion glass slide which comprises a glass slide body, the glass slide body comprises a bottom layer, a first layer, a nano coating, a molecular modification layer, a middle layer and a liquid-based layer, the first layer is located on the surface of the bottom layer, the nano coating is located on the surface of the first layer, and the molecular modification layer is located on the surface of the middle layer. The molecular modification layer is located on the surface of the nano coating, the middle layer is located on the surface of the molecular modification layer, and the liquid base layer is located on the surface of the middle layer. According to the utility model, a multi-layer structural design is adopted, the glass material at the bottom layer provides stable support, the silicon dioxide layer enhances the surface smoothness and corrosion resistance, the polymer nano coating improves the durability and adhesive force, and the molecular modification layer of the bioactive polypeptide enhances the biological recognition function and improves the specificity and sensitivity of biological detection; and the liquid base layer of the macromolecular humectant keeps the sample wet and enhances the treatment stability.

Description

Technical Field

[0001] The utility model relates to the technical field of glass slides, in particular to a novel liquid-based adhesive glass slide. Background Art

[0002] The liquid-based cytology technology used in recent years is significantly better than ordinary Pap smears in sampling. It is a more accurate and useful method for cervical cancer screening, and plays an important auxiliary role in clinical diagnosis and treatment, efficacy observation, and prognosis judgment. There are many kinds of liquid-based adhesive slides on the market, but the adhesion performance of the products varies. The slides often lack sufficient chemical modification layers, which limits their application in the biomedical field. Due to the lack of a special biorecognition layer, the sensitivity and specificity of the slides in the detection of complex biological samples are insufficient. In addition, the surface treatment technology of conventional slides is not enough to effectively control the nanostructure, resulting in adhesion and mechanical stability problems. These problems are particularly obvious during long-term storage or complex operations. In actual operations, they may lead to unreliable and poor repeatability of sample processing, thereby affecting the accuracy and practicality of the final test results, which need to be improved. Utility Model Content

[0003] The purpose of the utility model is to solve the technical problems raised in the above background technology.

[0004] The utility model adopts the following technical scheme: a novel liquid-based adhesive glass slide, comprising a glass slide body, wherein the glass slide body comprises a bottom layer, a first layer, a nano-coating, a molecular modification layer, an intermediate layer and a liquid-based layer, wherein the first layer is located on the surface of the bottom layer, the nano-coating is located on the surface of the first layer, the molecular modification layer is located on the surface of the nano-coating, the intermediate layer is located on the surface of the molecular modification layer, and the liquid-based layer is located on the surface of the intermediate layer.

[0005] Preferably, the material used for the bottom layer is glass.

[0006] Preferably, the material used for the first layer is silicon dioxide.

[0007] Preferably, the nanocoating is composed of polymer nanoparticles and is prepared by a solution process.

[0008] Preferably, the molecular modification layer uses oxygen plasma to increase the functional groups on the surface, and uses a silane coupling agent to form a self-assembled monolayer on the surface of the slide to fix the polypeptide.

[0009] Preferably, the intermediate layer is made of a polymer film.

[0010] Preferably, the liquid-based layer is coated on the surface of the glass slide using a polymer moisturizing agent, and the polymer moisturizing agent is polyethylene glycol.

[0011] Compared with the prior art, the advantages and positive effects of the utility model are:

[0012] In the utility model, a multi-layer structure design is adopted, the glass material of the bottom layer provides a stable and solid supporting platform, the first layer of silicon dioxide enhances the smoothness and chemical corrosion resistance of the surface, and helps to evenly coat the subsequent layer materials, the nano coating is composed of polymer nanoparticles, which not only improves the overall durability of the structure, but also improves the adhesion and biocompatibility of the slide through microscopic structural regulation, the molecular modification layer uses bioactive polypeptides to enhance the biorecognition function, so that the specificity and sensitivity of the slide in biological detection and medical applications are greatly improved, and the liquid base layer of the polymer moisturizer effectively maintains the wet state of the sample, and improves the stability and reliability during the sample processing process. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 A schematic diagram of a novel liquid-based adhesive slide is provided for the utility model;

[0014] Figure 2 A cross-sectional view of a novel liquid-based adhesive slide is proposed for the utility model;

[0015] Figure 3 A novel process flow chart of liquid-based adhesive slide is proposed for the utility model.

[0016] Legend:

[0017] 1. Slide body; 2. Bottom layer; 3. First layer; 4. Nano coating; 5. Molecular modification layer; 6. Middle layer; 7. Liquid base layer. DETAILED DESCRIPTION

[0018] In order to more clearly understand the above-mentioned purpose, features and advantages of the utility model, the utility model is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0019] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0020] Example

[0021] See also Figure 1-2The utility model provides a technical solution: a new type of liquid-based adhesive glass slide, including a glass slide body 1, the glass slide body 1 includes a bottom layer 2, a first layer 3, a nano coating 4, a molecular modification layer 5, an intermediate layer 6 and a liquid base layer 7, the material used for the bottom layer 2 is glass, and the glass has good optical properties and biocompatibility, the first layer 3 is located on the surface of the bottom layer 2, the material used for the first layer 3 is silicon dioxide, which can improve the wettability and adhesion of the surface, the nano coating 4 is located on the surface of the first layer 3, the nano coating 4 is composed of polymer nanoparticles, and is prepared by a solution method to enhance the surface roughness and effective surface area, thereby improving the adhesion of cells and samples. The molecular modification layer 5 is located on the surface of the nano coating 4, and the molecular modification layer 5 uses oxygen plasma to increase the functional groups on the surface, and uses a silane coupling agent to form a self-assembled monolayer on the surface of the glass slide to fix the polypeptide to achieve specific cell recognition, interaction or manipulation functions. The middle layer 6 is located on the surface of the molecular modification layer 5, and the middle layer 6 uses a polymer film to further enhance the interaction between the glass slide and the cell sample and provide more adhesion and stability. The liquid base layer 7 is located on the surface of the middle layer 6, and the liquid base layer 7 is coated on the surface of the glass slide with a polymer moisturizer. The polymer moisturizer is polyethylene glycol, which provides a protective layer for the infiltrated sample.

[0022] The utility model discloses a novel liquid-based adhesive glass slide. It is coated with an adhesive agent on a conventional glass slide, and then cleaned and dried to obtain a novel liquid-based adhesive glass slide. Optical-grade glass is selected as the bottom material. Borosilicate glass is commonly selected. The thickness is generally 1.0 mm. The glass substrate is polished and ultrasonically cleaned with ethanol and distilled water to ensure that the surface is dust-free and smooth. Subsequently, a silicon dioxide layer of about 50 nm thick is coated on the glass surface by sol-gel technology. The precursor used is tetraethyl orthosilicate (TEOS), and it is baked at 150°C for 2 hours to solidify. The nano-coating is prepared by solution polymerization of polystyrene (PS) nanoparticles with a particle diameter of about 100 nm. It is applied by dip coating or spin coating to increase the surface roughness of the glass slide to improve the attachment of cells and samples. After the surface of the nano-coating is treated with oxygen plasma, a 0.5% 3-aminopropyltriethoxysilane (APTES) solution is used to coat the surface of the glass slide. The surface was left to stand at room temperature for 12 hours to achieve surface functionalization and increase functional groups to provide support for the fixation of polypeptides, thereby forming a molecular modification layer. Polyvinyl alcohol (PVA) was applied to the surface of the molecular modification layer by spin coating with a thickness of about 20 μm to form an intermediate layer, which can enhance the interaction and adhesion with the cell sample. Polyethylene glycol (PEG) with a molecular weight of 4000 Da was sprayed on the surface of the intermediate layer with a coating thickness of about 300 μm to provide a lasting moisturizing effect, provide a protective layer for the sample, and optimize the sample processing and storage conditions.

[0023] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A novel liquid-based adhesive glass slide, comprising a glass slide body (1), characterized in that: The glass slide body (1) comprises a bottom layer (2), a first layer (3), a nano coating (4), a molecular modification layer (5), an intermediate layer (6) and a liquid base layer (7), wherein the first layer (3) is located on the surface of the bottom layer (2), the nano coating (4) is located on the surface of the first layer (3), the molecular modification layer (5) is located on the surface of the nano coating (4), the intermediate layer (6) is located on the surface of the molecular modification layer (5), and the liquid base layer (7) is located on the surface of the intermediate layer (6).

2. The novel liquid-based adhesive slide according to claim 1 is characterized in that: The material used for the bottom layer (2) is glass.

3. The novel liquid-based adhesive slide according to claim 1 is characterized in that: The material used for the first layer (3) is silicon dioxide.

4. The novel liquid-based adhesive slide according to claim 1 is characterized in that: The nano coating (4) is composed of polymer nanoparticles and is prepared by a solution method.

5. The novel liquid-based adhesive slide according to claim 1 is characterized in that: The molecular modification layer (5) uses oxygen plasma to increase the functional groups on the surface, and uses a silane coupling agent to form a self-assembled monolayer on the surface of the slide to fix the polypeptide.

6. The novel liquid-based adhesive slide according to claim 1 is characterized in that: The intermediate layer (6) is made of a polymer film.

7. The novel liquid-based adhesive slide according to claim 1 is characterized in that: The liquid base layer (7) is coated on the surface of the glass slide using a polymer moisturizing agent, and the polymer moisturizing agent is polyethylene glycol.

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