Convenient tumor tissue cleaning device for 3D (three-dimensional) organoid culture

Abstract

The utility model relates to the technical field of tumor tissue cleaning, and particularly discloses a convenient tumor tissue cleaning device for 3D (three-dimensional) organoid culture, which comprises an operation table, a cleaning device and a control system, the rotary seat is cylindrical on the whole and is rotationally arranged on the mounting frame by taking a column core as a shaft; the through valves are arranged on the operation table, the through valves are tightly attached to the circumferential face of the rotary seat to form movable connection, water inlets of the through valves communicate with the cleaning fluid storage bottle A, and water outlets of the through valves are located in the sides, attached to the circumferential face of the rotary seat, of the through valves; a water inlet of the suction pump is communicated with a water outlet of a liquid guide cavity in the rotary seat, and a water inlet of the liquid guide cavity is formed in the circumferential surface of the rotary seat and is opposite to a water outlet of one of the through valves. According to the utility model, the suction pump can be quickly switched to be communicated with different cleaning liquid storage bottles A, the switching operation is convenient, and the cleaning efficiency of tumor tissues is indirectly improved.

Description

Technical Field

[0001] The utility model belongs to the field of tumor tissue cleaning, and specifically relates to a convenient tumor tissue cleaning device for 3D organoid culture. Background Art

[0002] Tumor organoid culture is a technology that uses patient-derived tumor tissue to culture 3D cell cultures in vitro. It can not only highly retain the 3D tissue structure similar to that of the corresponding tumor when it grows in vivo, but also retain the patient's biological genetic information and its heterogeneity after multiple passages, which can provide a strong basis and support for the prediction and formulation of subsequent precise individualized treatment strategies for patients. This fully makes up for the defects of the 2D cell line culture system, such as the easy loss of tumor heterogeneity during long-term culture, and its inability to simulate the complex structure and pathological characteristics of tumors in patients.

[0003] At present, the pathological study of tumor tissue specimens has an important impact on the treatment of patients. The collected tumor tissue generally needs to be cleaned to remove excess tissues such as mesentery and blood vessels, and the patient-derived bacteria are removed by cleaning fluid for research use. A Chinese patent with publication number CN214718735U discloses a flushing device for tumor tissue chemical staining films. The flushing device uses a servo motor installed in a power cavity and an auxiliary gear to make the staining film clamping mechanism installed in the cleaning cavity rotate and swing, and can evenly spray and flush different types of tumor tissue chemical staining films clamped;

[0004] However, different types or concentrations of tissue cleaning fluids are usually used for cleaning different tumor tissues or at different steps. The existing tumor tissue cleaning device has a single pipeline. When cleaning different tumor tissues or at different steps, the pipeline needs to be manually connected to different cleaning fluid storage bottles, which is inconvenient to operate. Utility Model Content

[0005] The purpose of the utility model is to provide a convenient tumor tissue cleaning device for 3D organoid culture to solve the problems raised in the above-mentioned background technology.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A convenient tumor tissue cleaning device for 3D organoid culture, comprising:

[0008] An operating table, on which a mounting frame is arranged;

[0009] The swivel seat is cylindrical in shape and is arranged on the mounting frame to rotate with the column core as the axis;

[0010] A plurality of through valves are provided and arranged on the operating table. The through valves are closely fitted to the circumference of the rotary seat to form a dynamic connection. The water inlet of the through valve is connected to the cleaning liquid storage bottle A. The water outlet of the through valve is located on the side of the through valve that fits the circumference of the rotary seat.

[0011] A suction pump, wherein the water inlet of the suction pump is connected to the water outlet of the liquid guiding cavity inside the rotary seat, and the water inlet of the liquid guiding cavity is arranged on the peripheral surface of the rotary seat and is opposite to the water outlet of one of the through valves;

[0012] The water inlet of the nozzle is connected with the water outlet of the suction pump through a conduit.

[0013] Preferably, the column core of the swivel seat is located vertically, and the swivel seat is driven to rotate by a driving unit.

[0014] Preferably, a bracket is arranged on the table top of the operating table, and the through valve is connected to the bracket.

[0015] Preferably, the water inlet of the through valve is connected to a connecting pipe, and the overhanging end of the connecting pipe extends to the inner bottom of the cleaning liquid storage bottle A.

[0016] Preferably, a sealing gasket is arranged on one side of the through valve that is in contact with the circumferential surface of the rotary seat, and the sealing gasket is in contact with the circumferential surface of the rotary seat to form a dynamic connection.

[0017] Preferably, a placement rack is provided on the table top of the operating table, and the nozzle is located on the placement rack.

[0018] Through the setting of the above technical scheme, a plurality of cleaning liquid storage bottles A are respectively used to store cleaning liquids (buffers) of different types or concentrations. When cleaning the tumor tissue, when different cleaning liquids are needed for cleaning, the rotating seat can be controlled to rotate. The circumferential surface of the rotating seat is deflected, and the water inlet of the liquid guide cavity located on the circumferential surface of the rotating seat rotates synchronously, and can be rotated to the required valve position connected to the cleaning liquid storage bottle A, so that the water inlet of the suction pump can be connected to the valve through the rotating seat, and then connected to the corresponding cleaning liquid storage bottle A. During cleaning, the cleaning liquid in the corresponding cleaning liquid storage bottle A can be sucked by controlling the suction pump to start, and the tumor tissue can be sprayed and cleaned using a nozzle. This solution can quickly switch the suction pump to be connected to different cleaning liquid storage bottles A, and the switching operation is convenient.

[0019] Compared with the prior art, the beneficial effect of the utility model is that through the cooperation between the rotating seat and a plurality of valves, when cleaning the tumor tissue, when different cleaning solutions are needed for cleaning, the rotating seat can be controlled to rotate, and then the suction pump can be quickly switched to connect with different cleaning solution storage bottles A, and the switching operation is convenient, which indirectly improves the cleaning efficiency of the tumor tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1This is a three-dimensional appearance diagram of the utility model;

[0021] Figure 2 It is a partial structural schematic diagram of the utility model;

[0022] Figure 3 It is a top view of the local structure of the utility model;

[0023] Figure 4 It is a partial structural sectional view of the utility model.

[0024] In the figure: 10, operating table; 20, nozzle; 30, mounting frame; 31, driving unit; 32, rotating seat; 321, liquid guiding cavity; 40, suction pump; 41, water inlet; 42, conduit; 50, bracket; 51, through valve; 52, through cavity; 53, connecting pipe; 54, sealing gasket; 60, placement rack. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Embodiment 1: A convenient tumor tissue cleaning device for 3D organoid culture, comprising: an operating table 10, on which a mounting frame 30 is arranged; a rotating seat 32, which is cylindrical as a whole and is arranged on the mounting frame 30 with the cylindrical core as the axis; a plurality of valves 51 are arranged on the operating table 10, the valves 51 are tightly fitted to the circumference of the rotating seat 32 to form a dynamic connection, the water inlet of the valve 51 is connected to the cleaning liquid storage bottle A, and the water outlet of the valve 51 is located on the side of the valve 51 that fits the circumference of the rotating seat 32; a suction pump 40, the water inlet 41 of the suction pump 40 is connected to the water outlet of the liquid guide cavity 321 inside the rotating seat 32, the water inlet of the liquid guide cavity 321 is opened on the circumference of the rotating seat 32 and is opposite to one of the water outlets of the valve 51; a nozzle 20, the water inlet of which is connected to the water outlet of the suction pump 40 through a conduit 42.

[0027] In the above scheme, a plurality of cleaning liquid storage bottles A are respectively used to store cleaning liquids (buffers) of different types or concentrations. When cleaning the tumor tissue, when different cleaning liquids are needed for cleaning, the rotating seat 32 can be controlled to rotate. The circumferential surface of the rotating seat 32 is deflected, and the water inlet of the liquid guide cavity 321 located on the circumferential surface of the rotating seat 32 rotates synchronously, and can be rotated to the position of the through valve 51 connected to the required cleaning liquid storage bottle A, so that the water inlet 41 of the suction pump 40 can be connected to the through valve 51 through the rotating seat 32, and then connected to the corresponding cleaning liquid storage bottle A. During cleaning, the cleaning liquid in the corresponding cleaning liquid storage bottle A can be sucked by controlling the suction pump 40 to start, and the tumor tissue can be sprayed and cleaned using the nozzle 20. This scheme can quickly switch the suction pump 40 to be connected to different cleaning liquid storage bottles A, and the switching operation is convenient.

[0028] Furthermore, the column core of the rotating seat 32 is located in the vertical direction, and the rotating seat 32 is driven to rotate by the driving unit 31. The driving unit 31 is preferably a servo motor, which can accurately control the rotation amount of the rotating seat 32.

[0029] In the solution, in order to ensure the stable arrangement of the through valve 51 , a bracket 50 is arranged on the table top of the operating table 10 , and the through valve 51 is connected to the bracket 50 .

[0030] The water inlet of the through valve 51 is connected to a connecting pipe 53, and the overhanging end of the connecting pipe 53 extends to the inner bottom of the cleaning liquid storage bottle A. The suction pump 40 can suck the cleaning liquid in the cleaning liquid storage bottle A through the swivel seat 32, the through cavity 52 inside the through valve 51 and the connecting pipe 53 in sequence.

[0031] As a preferred solution, in order to facilitate the fixed-point storage of the nozzle 20 , a placement rack 60 is provided on the table top of the operating table 10 , and the nozzle 20 is located on the placement rack 60 .

[0032] Embodiment 2: The difference between this embodiment and embodiment 1 is that, in order to ensure the sealing performance between the through valve 51 and the circumference of the rotating seat 32, a sealing gasket 54 is arranged on the side of the through valve 51 that is in contact with the circumference of the rotating seat 32, and the sealing gasket 54 is in contact with the circumference of the rotating seat 32 and forms a dynamic connection. In this way, when the cleaning liquid is transported, it can be ensured that the movable gap between the through valve 51 and the circumference of the guide tube 42 will not leak.

[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A convenient tumor tissue cleaning device for 3D organoid culture, characterized in that: include: An operating table (10) having a mounting frame (30) arranged on the table top; The swivel seat (32) is cylindrical in shape and is rotatably arranged on the mounting frame (30) with the core of the column as an axis; A plurality of through valves (51) are provided and arranged on the operating table (10); the through valves (51) are closely fitted to the circumference of the rotating seat (32) to form a dynamic connection; a water inlet of the through valve (51) is connected to the cleaning liquid storage bottle A; and a water outlet of the through valve (51) is located on a side of the through valve (51) that is fitted to the circumference of the rotating seat (32); A suction pump (40), wherein a water inlet (41) of the suction pump (40) is connected to a water outlet of a liquid guiding cavity (321) inside the rotating seat (32), and the water inlet of the liquid guiding cavity (321) is arranged on the peripheral surface of the rotating seat (32) and is opposite to a water outlet of one of the through valves (51); The water inlet of the spray head (20) is connected to the water outlet of the suction pump (40) through a conduit (42).

2. A convenient tumor tissue cleaning device for 3D organoid culture according to claim 1, characterized in that: The column core of the rotary seat (32) is located in the vertical direction, and the rotary seat (32) is driven to rotate by the driving unit (31).

3. The convenient tumor tissue cleaning device for 3D organoid culture according to claim 1, characterized in that: A bracket (50) is arranged on the table top of the operating table (10), and a through valve (51) is connected to the bracket (50).

4. A convenient tumor tissue cleaning device for 3D organoid culture according to claim 1 or 3, characterized in that: The water inlet of the through valve (51) is connected to a connecting pipe (53), and the overhanging end of the connecting pipe (53) extends to the inner bottom of the cleaning liquid storage bottle A.

5. The convenient tumor tissue cleaning device for 3D organoid culture according to claim 1, characterized in that: A sealing gasket (54) is arranged on one side of the through valve (51) that is in contact with the circumferential surface of the rotating seat (32). The sealing gasket (54) is in contact with the circumferential surface of the rotating seat (32) to form a dynamic connection.

6. The convenient tumor tissue cleaning device for 3D organoid culture according to claim 1, characterized in that: A placement rack (60) is arranged on the table top of the operating table (10), and the spray head (20) is located on the placement rack (60).

Images