Three-dimensional culture device for tumor stem cells

Abstract

The utility model relates to the technical field of stem cell culture, and discloses a tumor stem cell three-dimensional culture device which comprises a container, a cover plate is arranged at the top of the container, a liquid adding pipe is fixedly connected to the end face of the cover plate in an inserted mode, the lower end of the liquid adding pipe fixedly communicates with a hollow plate, and a heating table is arranged in the hollow plate; the interior of the heating table is hollow, an electric heater is fixedly installed in the heating table, the container and the heating table are jointly provided with a circulation heating mechanism, the circulation heating mechanism and the heating table are both filled with heat conduction liquid, and a plurality of liquid inlet holes are formed in the bottom of the hollow plate. The temperature difference between the culture medium and the environment can be effectively reduced, and it is prevented that when the culture medium is added, the environment temperature becomes low, and culture of tumor stem cells is affected.

Description

Technical Field

[0001] The present application relates to the technical field of stem cell culture, and in particular to a three-dimensional tumor stem cell culture device. Background Art

[0002] Three-dimensional stem cell culture is a method of culturing stem cells. Different from traditional two-dimensional culture, it provides a three-dimensional structure that is closer to the in vivo environment, providing better conditions for the growth and differentiation of stem cells.

[0003] At present, when stem cells are cultured in three dimensions, culture medium needs to be added to the vessel. For example, a three-dimensional tumor stem cell culture device disclosed in patent publication number CN213506972U adds culture medium to the vessel through a special feeding funnel and a pipe. However, the culture medium is usually in a low-temperature state before being added, and during the stem cell culture process, the inside of the vessel needs to be kept in a suitable constant temperature state. After the culture medium is added, the temperature inside the vessel will drop rapidly due to the added culture medium. The temperature can only be restored by continuing to heat the vessel through a heating device. However, temperature recovery takes a certain amount of time, and sudden temperature changes are not conducive to the culture of stem cells. Utility Model Content

[0004] The purpose of the present application is to solve the problem in the prior art that after the culture medium is added, the temperature inside the vessel will drop rapidly due to the added culture medium, and the temperature can only be restored by continuing to heat the vessel through a heating device, but the temperature recovery takes a certain amount of time, and sudden temperature changes are not conducive to the cultivation of stem cells. A three-dimensional culture device for tumor stem cells is proposed.

[0005] In order to achieve the above purpose, this application adopts the following technical solutions:

[0006] A three-dimensional tumor stem cell culture device comprises a container, wherein a cover plate is provided on the top of the container, a liquid adding tube is fixedly inserted on the end surface of the cover plate, a lower end of the liquid adding tube is fixedly connected to a hollow plate, a heating platform is provided inside the hollow plate, the interior of the heating platform is hollow, and an electric heater is fixedly installed inside the heating platform, a circulating heating mechanism is installed together with the heating platform, and the interiors of the circulating heating mechanism and the heating platform are both filled with heat transfer liquid, and a plurality of liquid inlet holes are provided at the bottom of the hollow plate.

[0007] Preferably, the circulating heating mechanism includes an annular heat exchange jacket fixedly mounted on the inner wall of the container, and the interior of the annular heat exchange jacket is hollow. A circulating pump is fixedly mounted on the outer wall of the container, and the suction end of the circulating pump is connected to the interior of the annular heat exchange jacket. An output end of the circulating pump is fixedly connected to an infusion pipe, and the infusion pipe is connected to the interior of the heating platform. The heating platform and the annular heat exchange jacket are fixedly connected to a reflux pipe.

[0008] Preferably, the lower end of the hollow plate is provided with two symmetrically arranged inclined surfaces, and each of the liquid inlet holes is evenly distributed on the inclined surface on the same side.

[0009] Preferably, the cross section of the heating platform is triangular.

[0010] Preferably, a heat insulation cover is fixedly mounted on the outer wall of the container, and the circulation pump, the infusion pipe and the reflux pipe are all arranged on the inner side of the heat insulation cover.

[0011] Preferably, two heat conducting plates are fixedly mounted on the side walls of the heating platform, and one side wall of the two heat conducting plates is in contact with the heat conducting liquid.

[0012] Compared with the prior art, the present application provides a three-dimensional tumor stem cell culture device, which has the following beneficial effects:

[0013] 1. The three-dimensional culture device for tumor stem cells can preheat the culture medium during the process of the culture medium entering the container through the cooperation of the container, the cover plate, the liquid adding tube, the hollow plate, the heating table, the electric heater, the heat transfer liquid and the liquid inlet hole, so that the culture medium is close to the temperature of the tumor stem cell culture environment in the container, thereby effectively reducing the temperature difference between the culture medium and the environment, and preventing the environment temperature from becoming low when the culture medium is added, thereby affecting the culture of tumor stem cells.

[0014] 2. The three-dimensional tumor stem cell culture device can utilize the electric heater and heat transfer fluid inside the heating platform through the circulating heating mechanism to culture the tumor stem cells at a suitable temperature during the tumor stem cell culture process.

[0015] Moreover, the parts not involved in the device are the same as the existing technology or can be implemented by using the existing technology. The present application can effectively reduce the temperature difference between the culture medium and the environment, and prevent the addition of the culture medium from causing the ambient temperature to drop and affecting the cultivation of tumor stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a three-dimensional tumor stem cell culture device proposed in this application;

[0017] Figure 2 This is a schematic cross-sectional view of a three-dimensional tumor stem cell culture device proposed in this application;

[0018] Figure 3 A three-dimensional tumor stem cell culture device proposed in this application Figure 2 A magnified view of the structure of part A.

[0019] In the figure: 1. container; 2. cover plate; 3. liquid adding pipe; 4. hollow plate; 5. heating table; 6. electric heater; 7. heat transfer liquid; 8. liquid inlet hole; 9. annular heat exchange jacket; 10. circulation pump; 11. liquid infusion pipe; 12. return pipe; 13. heat insulation cover; 14. heat transfer plate. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments.

[0021] Reference Figure 1-3 A three-dimensional culture device for tumor stem cells includes a container 1, a cover plate 2 is provided on the top of the container 1, a liquid adding tube 3 is fixedly inserted on the end surface of the cover plate 2, a hollow plate 4 is fixedly connected to the lower end of the liquid adding tube 3, a heating platform 5 is provided inside the hollow plate 4, the interior of the heating platform 5 is hollow, and an electric heater 6 is fixedly installed inside the heating platform 5, a circulating heating mechanism is installed together with the heating platform 5, and the circulating heating mechanism and the interior of the heating platform 5 are filled with heat transfer liquid 7, and a plurality of liquid inlet holes 8 are opened at the bottom of the hollow plate 4.

[0022] The circulating heating mechanism includes an annular heat exchange sleeve 9 fixedly mounted on the inner wall of the container 1, and the interior of the annular heat exchange sleeve 9 is hollow. A circulating pump 10 is fixedly mounted on the outer wall of the container 1, and the suction end of the circulating pump 10 is connected to the interior of the annular heat exchange sleeve 9. The output end of the circulating pump 10 is fixedly connected to an infusion tube 11, and the infusion tube 11 is connected to the interior of the heating platform 5. The heating platform 5 and the annular heat exchange sleeve 9 are fixedly connected to a return pipe 12. Through this arrangement, the electric heater 6 and the heat transfer fluid 7 inside the heating platform 5 can be used to assist in culturing tumor stem cells at a suitable temperature. A special temperature controller is installed inside the container 1 to control the operation of the electric heater 6. The temperature controller can monitor the temperature inside the container 1 and adjust the heat emitted by the heater 6 according to the temperature. This is an existing mature technology, so it will not be described here.

[0023] The lower end of the hollow plate 4 is provided with two symmetrically arranged inclined surfaces, and each liquid inlet hole 8 is evenly distributed on the inclined surface on the same side. This arrangement can prevent the culture medium from accumulating inside the hollow plate 4.

[0024] The cross section of the heating platform 5 is arranged in a triangular shape, and this arrangement can avoid the culture medium from accumulating on the end surface of the heating platform 5 as much as possible.

[0025] The outer wall of the container 1 is fixedly mounted with a heat insulation cover 13, and the circulation pump 10, the liquid infusion pipe 11, and the return pipe 12 are all arranged on the inner side of the heat insulation cover 13. This arrangement can reduce the temperature loss of the heat transfer liquid 7 during the circulation flow.

[0026] Two heat conducting plates 14 are fixedly mounted on the side walls of the heating platform 5, and one side wall of the two heat conducting plates 14 is in contact with the heat conducting liquid 7. This arrangement can improve the heat exchange efficiency between the culture medium and the heat conducting liquid 7.

[0027] In the present application, when in use, before adding the culture medium, the electric heater 6 is started. At this time, the heat generated by the electric heater 6 will be transferred to the outer surface of the heating platform 5 through the heat transfer fluid 7. After waiting for not less than 3 minutes, the culture medium is added through the liquid adding tube 3. The culture medium will flow along the liquid adding tube 3 to the heating platform 5. The culture medium contacts the outer surface of the heating platform 5, so that heat can be exchanged with the heating platform 5. At this time, the temperature of the culture medium will rise. The culture medium after heat exchange enters the container 1 through the liquid inlet hole 8 at the bottom of the hollow plate 4 and contacts the tumor stem cells. Since the culture medium is pre-heated, the temperature difference between the culture medium and the environment in which the tumor stem cells are cultured can be reduced.

[0028] The above is only a preferred specific implementation manner of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can make equivalent substitutions or changes within the technical scope disclosed in the present application according to the technical solution and utility model concept of the present application, which should be covered by the protection scope of the present application.

Claims

1. A three-dimensional tumor stem cell culture device, comprising a container (1), characterized in that: The top of the container (1) is provided with a cover plate (2), the end surface of the cover plate (2) is fixedly plugged with a liquid adding pipe (3), the lower end of the liquid adding pipe (3) is fixedly connected to a hollow plate (4), a heating platform (5) is provided inside the hollow plate (4), the interior of the heating platform (5) is hollow, and an electric heater (6) is fixedly installed inside the heating platform (5), the container (1) and the heating platform (5) are jointly provided with a circulating heating mechanism, and the interiors of the circulating heating mechanism and the heating platform (5) are both filled with heat transfer liquid (7), and a plurality of liquid inlet holes (8) are provided at the bottom of the hollow plate (4).

2. A three-dimensional tumor stem cell culture device according to claim 1, characterized in that: The circulating heating mechanism comprises an annular heat exchange jacket (9) fixedly mounted on the inner wall of a container (1), and the interior of the annular heat exchange jacket (9) is hollow. A circulating pump (10) is fixedly mounted on the outer wall of the container (1), and the suction end of the circulating pump (10) is connected to the interior of the annular heat exchange jacket (9). The output end of the circulating pump (10) is fixedly connected to a liquid infusion pipe (11), and the liquid infusion pipe (11) is connected to the interior of a heating platform (5). The heating platform (5) and the annular heat exchange jacket (9) are fixedly connected to a return pipe (12).

3. The three-dimensional tumor stem cell culture device according to claim 1, characterized in that: The lower end of the hollow plate (4) is provided with two symmetrically arranged inclined surfaces, and each of the liquid inlet holes (8) is evenly distributed on the inclined surface on the same side.

4. A three-dimensional tumor stem cell culture device according to claim 3, characterized in that: The cross section of the heating platform (5) is arranged in a triangular shape.

5. The three-dimensional tumor stem cell culture device according to claim 2, characterized in that: A heat insulation cover (13) is fixedly mounted on the outer wall of the container (1), and the circulation pump (10), the liquid infusion pipe (11), and the return pipe (12) are all arranged on the inner side of the heat insulation cover (13).

6. The three-dimensional tumor stem cell culture device according to claim 1, characterized in that: Two heat-conducting plates (14) are fixedly mounted on the side walls of the heating platform (5), and one side wall of the two heat-conducting plates (14) is in contact with the heat-conducting liquid (7).

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