Portable sample detector

Abstract

The portable sample detector comprises a detector body, a display screen and a plurality of groups of buttons are mounted on the outer wall of the detector body, a liquid inlet end and a liquid outlet end are fixedly connected to the outer walls of the two sides of the detector body respectively, a detection cavity is formed in the detector body, a peristaltic pump and a flowing chamber are mounted in the detection cavity, and the peristaltic pump is connected with the flowing chamber. And a fixing device for fixing the magnet is mounted on the outer wall of the side plate. According to the utility model, devices such as the pin and the side plate are arranged, the fixing hole is formed in the outer wall of the magnet, and the pin is inserted into the fixing hole, so that the magnet can be fixed, and when the magnetism of the magnet is weak, the pin can be pulled out of the fixing hole so as to release the fixation of the magnet; when the magnet is replaced, the pin can be conveniently pulled out of the fixing hole, fixing of the magnet is relieved, then the magnet is replaced with a new magnet, and due to the detachable design, the magnet can be replaced easily and rapidly without complex operation or tools.

Description

Technical Field

[0001] The utility model relates to the technical field of detectors, in particular to a portable sample detector. Background Art

[0002] Portable sample detectors are mobile, easy-to-carry devices used to detect specific analytes in biological samples, environmental samples, or other types of samples. Portable sample detectors are usually designed for rapid, on-site, or instant testing. They can be used to detect a variety of analytes, such as pathogens, chemicals, pollutants, drug residues, etc. These detectors are often used in medical diagnosis, environmental monitoring, food safety testing, industrial quality control, and other fields.

[0003] Most of the detectors in the field of immunoassay and biomolecule detection currently on the market require the use of magnets to fix the magnetic beads at a specific position on the surface of the working electrode during detection. This can ensure stable contact between the magnetic beads and the electrode for subsequent detection and analysis. However, there are certain defects in actual use. For example, during use, magnets often need to be used in conjunction with electrodes. During the repeated magnetization and demagnetization of the magnets, material fatigue will occur, thereby gradually weakening the magnetism. As a result, the fixation of the magnetic beads on the electrode surface is unstable, affecting the sensitivity of the detection. Therefore, it is necessary to redesign a portable sample detector to address the above problems. Utility Model Content

[0004] The utility model aims to solve the shortcomings in the prior art and provides a portable sample detector.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A portable sample detector, including a detector body, a display screen and a plurality of buttons are installed on the outer wall of the detector body, a liquid inlet and a liquid outlet are fixedly connected to the outer walls on both sides of the detector body, a detection chamber is opened in the detector body, a peristaltic pump and a flow chamber are installed in the detection chamber, a reaction chamber is installed in the flow chamber, a working electrode and two groups of electrodes are installed on the outer wall of the flow chamber, the two groups of electrodes are symmetrically arranged on the outer wall of the flow chamber, a spring is installed on the outer wall of the working electrode, and a magnet is sleeved on the spring on the outer wall, a side plate is fixedly connected to the outer wall of the flow chamber, and a fixing device for fixing the magnet is installed on the outer wall of the side plate;

[0007] The fixing device comprises a pin slidably connected to the outer wall of the side plate, a fixing hole is opened on the upper end surface of the magnet, the end of the pin is slidably connected in the fixing hole and contacts the inner wall of the fixing hole, and an elastic mechanism is installed on the outer wall of the side plate.

[0008] Preferably, the elastic mechanism comprises a second spring fixedly connected to the upper end surface of the side plate, and the other end of the second spring is fixedly connected to the pin.

[0009] Preferably, a first injection port and a second injection port are provided on an outer wall of the liquid inlet end, and both the first injection port and the second injection port are connected to a peristaltic pump.

[0010] Preferably, the peristaltic pump is connected to the interior of the reaction chamber through two groups of connecting tubes, and the liquid outlet is connected to the interior of the reaction chamber.

[0011] Preferably, a photomultiplier tube is installed on the outer wall of the flow chamber.

[0012] The utility model has the following beneficial effects:

[0013] 1. The utility model provides devices such as pins and side plates, and opens a fixing hole on the outer wall of the magnet. The pin is inserted into the fixing hole to fix the magnet. When the magnetism of the magnet weakens, the pin can be pulled out of the fixing hole to release the fixation of the magnet. In this way, when the magnetism of the magnet weakens, the pin can be easily pulled out of the fixing hole to release the fixation of the magnet, and then a new magnet can be replaced. This detachable design makes it simple and quick to replace the magnet without complicated operations or tools.

[0014] 2. The utility model is provided with spring 2, side plate and other devices. When the magnet is replaced, the pin is pulled outward, and spring 2 is in a stretched state. When the replacement is completed, the pin is released, and the spring is restored to drive the pin to slide into the fixing hole. The reset force of the spring can ensure the tight connection between the pin and the fixing hole, providing a reliable fixing effect. This can prevent the magnet from loosening or falling off during use, and improve the stability and accuracy of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a structural schematic diagram of a portable sample detector proposed by the utility model;

[0016] Figure 2 for Figure 1 Section view.

[0017] Figure 3 for Figure 1 Schematic diagram of the structure of the peristaltic pump and flow chamber.

[0018] Figure 4 for Figure 3 Schematic diagram of the internal structure.

[0019] Figure 5 for Figure 3 A schematic diagram of the enlarged structure in the middle.

[0020] In the figure: 1 detector body, 2 button, 3 display screen, 4 liquid outlet, 5 liquid inlet, 6 first injection port, 7 second injection port, 8 detection chamber, 9 peristaltic pump, 10 flow chamber, 11 photomultiplier tube, 12 fixing hole, 13 working electrode, 14 spring 1, 15 magnet, 16 electrode, 17 side plate, 18 pin, 19 spring 2, 20 reaction chamber. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0022] Reference Figure 1-5 A portable sample detector comprises a detector body 1, a display screen 3 and a plurality of groups of buttons 2 are installed on the outer wall of the detector body 1, a liquid inlet 5 and a liquid outlet 4 are fixedly connected to the outer walls of both sides of the detector body 1, a detection chamber 8 is opened in the detector body 1, a peristaltic pump 9 and a flow chamber 10 are installed in the detection chamber 8, a reaction chamber 20 is installed in the flow chamber 10, a working electrode 13 and two groups of electrodes 16 are installed on the outer wall of the flow chamber 10, the two groups of electrodes 16 are symmetrically arranged on the outer wall of the flow chamber 10, a spring 14 is installed on the outer wall of the working electrode 13, and a magnet 15 is sleeved on the spring on the outer wall, a side plate 17 is fixedly connected to the outer wall of the flow chamber 10, and a fixing device for fixing the magnet 15 is installed on the outer wall of the side plate 17;

[0023] The fixing device includes a pin 18 slidably connected to the outer wall of the side plate 17. A fixing hole 12 is opened on the upper end surface of the magnet 15. The end of the pin 18 is slidably connected in the fixing hole 12 and contacts the inner wall of the fixing hole 12. An elastic mechanism is installed on the outer wall of the side plate 17.

[0024] The elastic mechanism includes a spring 19 fixedly connected to the upper end surface of the side plate 17, and the other end of the spring 19 is fixedly connected to the pin 18. The outer wall of the liquid inlet end 5 is provided with a first injection port 6 and a second injection port 7. The first injection port 6 and the second injection port 7 are both connected to the peristaltic pump 9. The peristaltic pump 9 is connected to the interior of the reaction chamber 20 through two groups of connecting pipes, and the liquid outlet end 4 is connected to the interior of the reaction chamber 20. A photomultiplier tube 11 is installed on the outer wall of the flow chamber 10.

[0025] In the utility model, when the magnetism of the magnet 15 weakens after being used for a period of time, the pin 18 can be pulled out from the fixing hole 12 by pulling the pin 18 upwards. At this time, the spring 2 19 is in a stretched state, so that the fixed limit of the magnet 15 can be released. After it is taken out from the outer wall of the spring 14, a new magnet 15 is replaced and plugged into the outer wall of the spring 14. The pin 18 is released, and the spring 2 19 is reset, driving the pin 18 to slide downward and plugged into the fixing hole 12 to fix the magnet 15. By regularly replacing the magnet 15, its magnetism is guaranteed to be in a fixed value range, thereby ensuring that the magnetic beads are fixed and stable on the surface of the electrode 16 to avoid affecting the sensitivity of the detection.

[0026] The following is the method of using this device during testing;

[0027] Step 1: The antibody to be detected that is conjugated with an activated terpyridine ruthenium derivative, namely [Ru(bpy)3]2++N-hydroxysuccinamide ester (NHS) and the antibody to be detected that is conjugated with biotin are added to a reaction cup together with the serum to be tested and incubated for 9 minutes.

[0028] Step 2: Add the streptavidin-coated magnetic beads into the reaction cup and incubate again for 9 minutes to allow biotin to bind to the avidin to connect the magnetic beads and the antibody to be detected to form a double antibody sandwich structure.

[0029] In the third step, the peristaltic pump 9 sucks the formed [Ru(bpy)3]2+-antibody-antigen-antibody-magnetic bead complex into the flow chamber 10. At this time, the magnetic beads are adsorbed to the surface of the electrode 16 by the magnet 15 under the working electrode 13. At the same time, the free antibodies to be detected (antibodies bound to biotin and [Ru(bpy)3]2+) are also sucked out of the reaction chamber 20.

[0030] Step 4: The peristaltic pump 9 adds a buffer solution containing tripropylamine (TPA), and at the same time, a voltage is applied to the electrode 16 to start the ECL reaction process. The luminescent agent [Ru(bpy)3]2+ and the electron donor TPA can each lose an electron at the same time on the anode surface to undergo an oxidation reaction, so that the divalent [Ru(bpy)3]2+ is oxidized to a trivalent one, the latter being a strong oxidant; on the other hand, TPA is oxidized to a cationic free radical TPA+●, which is very unstable and can spontaneously lose a proton (H+) to form a free radical TPA●, which is a very strong reducing agent that can donate an electron to the trivalent [Ru(bpy)3]3+ to form an excited state of [Ru(bpy)3]2+, and TPA itself is oxidized to dipropylamine and propionaldehyde.

[0031] The excited state [Ru(bpy)3]2+ decays through the fluorescence mechanism, emitting a photon with a wavelength of 620nm, and regenerating the ground state [Ru(bpy)3]2+. This process is repeated on the surface of the electrode 16, generating many photons, and the photomultiplier tube 11 detects the light intensity. The light intensity is linearly related to the concentration of [Ru(bpy)3]2+, so the content of the antigen to be detected, that is, the substance to be detected, can be measured.

[0032] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A portable sample detector, comprising a detector body (1), characterized in that: The outer wall of the detector body (1) is provided with a display screen (3) and a plurality of groups of buttons (2); the outer walls on both sides of the detector body (1) are respectively fixedly connected with a liquid inlet (5) and a liquid outlet (4); a detection chamber (8) is provided in the detector body (1); a peristaltic pump (9) and a flow chamber (10) are installed in the detection chamber (8); a reaction chamber (20) is installed in the flow chamber (10); a working electrode (13) and two groups of electrodes (16) are installed on the outer wall of the flow chamber (10); the two groups of electrodes (16) are symmetrically arranged on the outer wall of the flow chamber (10); a spring (14) is installed on the outer wall of the working electrode (13); and a magnet (15) is sleeved on the spring; a side plate (17) is fixedly connected to the outer wall of the flow chamber (10); a fixing device for fixing the magnet (15) is installed on the outer wall of the side plate (17); The fixing device comprises a pin (18) slidably connected to the outer wall of the side plate (17); a fixing hole (12) is provided on the upper end surface of the magnet (15); an end of the pin (18) is slidably connected in the fixing hole (12) and contacts the inner wall of the fixing hole (12); and an elastic mechanism is installed on the outer wall of the side plate (17).

2. A portable sample detector according to claim 1, characterized in that: The elastic mechanism comprises a second spring (19) fixedly connected to the upper end surface of the side plate (17), and the other end of the second spring (19) is fixedly connected to the pin (18).

3. A portable sample detector according to claim 2, characterized in that: The outer wall of the liquid inlet end (5) is provided with a first injection port (6) and a second injection port (7), and the first injection port (6) and the second injection port (7) are both connected to a peristaltic pump (9).

4. A portable sample detector according to claim 3, characterized in that: The peristaltic pump (9) is connected to the interior of the reaction chamber (20) via two sets of connecting pipes, and the liquid outlet end (4) is also connected to the interior of the reaction chamber (20).

5. A portable sample detector according to claim 4, characterized in that: A photomultiplier tube (11) is installed on the outer wall of the flow chamber (10).

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