Rapid extraction device for recombinant protein

Abstract

The utility model relates to the technical field of biology, and discloses a rapid extraction device for recombinant protein, which comprises a stabilizing block, the inner wall of the stabilizing block is rotatably connected with a rotating shaft, the top end of the rotating shaft is fixedly connected with a rotating disc, the outer wall of a bidirectional threaded rod is in threaded connection with a clamping block, and the inner wall of a connecting disc is fixedly connected with a supporting plate. And the outer wall of the clamping block is slidably connected to the outer wall of the supporting plate, the outer wall of the clamping block is slidably connected to the interior of the inserting groove, a mounting groove is formed in the centrifugal disc, a connecting plate is fixedly connected to the outer wall of the stabilizing block, and a driving assembly is arranged on the outer wall of the connecting plate. According to the device disclosed by the utility model, the rotating disc drives the connecting disc to perform centrifugation so as to perform centrifugal extraction on recombinant protein, the motor drives the bidirectional threaded rod to quickly fix the centrifugal disc through the clamping block, and the centrifugal disc is provided with a plurality of mounting grooves, so that a plurality of test tubes filled with materials can be put in the mounting grooves to perform extraction in the use process.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a rapid extraction device for recombinant proteins. Background Art

[0002] Recombinant proteins refer to proteins synthesized in heterologous expression systems through genetic engineering technology. These proteins are usually produced by modifying genes obtained from other species and expressing them in host cells. Rapid extraction devices are usually designed to efficiently extract target proteins from a large number of samples. They can process a large number of samples in a short time, improving the efficiency and yield of extraction, which is especially important for applications that require a large amount of protein. Cellular components such as cell fragments and cell nuclei can be separated from dissolved proteins by centrifugation. High-speed centrifugation can extract proteins in the supernatant.

[0003] During the use of most devices, when encountering molds for shells of various materials, the device may become unusable or unable to achieve the effect of rapid extraction. During the use process, the technicians will be required to operate it all the time and it will be impossible to extract according to a variety of different loading bottles. Utility Model Content

[0004] In order to remedy the above deficiencies, the utility model provides a rapid extraction device for recombinant proteins, aiming to improve the problem that when encountering molds for shells of various materials, the device may not be able to be used or cannot achieve rapid extraction. During use, the technicians need to operate it all the time and it is impossible to extract according to a variety of different loading bottles.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A rapid extraction device for recombinant protein comprises a stabilizing block, the inner wall of the stabilizing block is rotatably connected to a rotating shaft, the top of the rotating shaft is fixedly connected to a rotating disk, the upper surface of the rotating disk is fixedly connected to a connecting disk, the outer wall of the connecting disk is fixedly connected to a motor, the output end of the motor is fixedly connected to a bidirectional threaded rod, the outer wall of the bidirectional threaded rod is threadedly connected to a clamping block, the inner wall of the connecting disk is fixedly connected to a supporting plate, the outer wall of the clamping block is slidably connected to the outer wall of the supporting plate, the upper surface of the supporting plate is slidably connected to a centrifugal disk, a slot is provided inside the centrifugal disk, the outer wall of the clamping block is slidably connected to the inside of the slot, an installation groove is provided inside the centrifugal disk, the outer wall of the stabilizing block is fixedly connected to the connecting plate, and the outer wall of the connecting plate is provided with a driving assembly.

[0007] Preferably, the driving assembly includes a first motor, an outer wall of the first motor is fixedly connected to the upper surface of the connecting plate, an output end of the first motor is fixedly connected to a first transmission roller, an outer wall of the first transmission roller is slidably connected to a belt, an outer wall of the belt is slidably connected to a second transmission roller, and an upper surface of the second transmission roller is fixedly connected to the bottom end of the rotating shaft.

[0008] Preferably, the outer wall of the connecting plate is fixedly connected to the outer shell, and the outer wall of the outer shell is fixedly connected to the hinge.

[0009] Preferably, the outer wall of the hinge is fixedly connected to a door panel, and the inner wall of the door panel is fixedly connected to a door handle.

[0010] Preferably, the outer wall of the door panel is slidably connected to the inner wall of the outer shell, and the inner wall of the outer shell is fixedly connected to an outer frame.

[0011] Preferably, a second motor is fixedly connected to an outer wall of the outer frame, and an output end of the second motor is fixedly connected to a first sprocket.

[0012] Preferably, the outer wall of the first sprocket is meshedly connected with a chain, and the outer wall of the chain is meshedly connected with a second sprocket.

[0013] Preferably, fans are fixedly connected to outer walls of the first sprocket and the second sprocket, and a dustproof net is fixedly connected to the inner wall of the outer frame.

[0014] The utility model has the following beneficial effects:

[0015] 1. In the utility model, the centrifugal extraction of recombinant protein can be performed by driving the connecting disk to centrifuge through the turntable, and the bidirectional threaded rod driven by the motor can quickly fix the centrifugal disk through the block, and there are multiple mounting grooves on the centrifugal disk. During use, multiple test tubes containing materials can be placed in for extraction.

[0016] 2. In the utility model, the first sprocket and the second sprocket are driven to move by the second motor, and the heat dissipated by the internal electronic components during long-term operation can be discharged by the fan. The dustproof net can prevent dust from entering the interior and polluting the internal electronic components during use.

[0017] 3. In the utility model, the centrifugal disc can be quickly installed and disassembled through the card block. When test tubes of different sizes need to be extracted during use, the centrifugal disc can be replaced by sliding the card block out from the inside of the slot. During use, it can better adapt to various extraction molds. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a three-dimensional diagram of the rapid extraction device for recombinant protein proposed by the utility model;

[0019] Figure 2 This is a schematic diagram of a belt of a rapid extraction device for recombinant protein proposed in the utility model;

[0020] Figure 3 This is a schematic diagram of a support plate of a rapid extraction device for recombinant protein proposed in the utility model;

[0021] Figure 4 This is a schematic diagram of the outer frame of the rapid extraction device for recombinant protein proposed by the utility model.

[0022] Legend:

[0023] 1. Connecting plate; 2. First motor; 3. First transmission roller; 4. Belt; 5. Second transmission roller; 6. Stabilizing block; 7. Rotating shaft; 8. Turntable; 9. Dust screen; 10. Connecting plate; 11. Motor; 12. Bidirectional threaded rod; 13. Block; 14. Support plate; 15. Centrifugal disc; 16. Slot; 17. Casing; 18. Hinge; 19. Door panel; 20. Door handle; 21. Outer frame; 22. Second motor; 23. First sprocket; 24. Chain; 25. Fan; 26. Second sprocket; 27. Mounting slot. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings of the specification of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Reference Figure 1-Figure 3The utility model provides an embodiment: a rapid extraction device for recombinant protein, comprising a stabilizing block 6, the inner wall of the stabilizing block 6 is rotatably connected with a rotating shaft 7, the top of the rotating shaft 7 is fixedly connected with a rotating disk 8, the upper surface of the rotating disk 8 is fixedly connected with a connecting disk 10, the outer wall of the connecting disk 10 is fixedly connected with a motor 11, the output end of the motor 11 is fixedly connected with a bidirectional threaded rod 12, the outer wall of the bidirectional threaded rod 12 is threadedly connected with a clamping block 13, the inner wall of the connecting disk 10 is fixedly connected with a supporting plate 14, the outer wall of the clamping block 13 is slidably connected to the outer wall of the supporting plate 14, and the upper surface of the supporting plate 14 is slidably connected with a centrifugal disk 15, a slot 16 is provided inside the centrifugal disc 15, the outer wall of the block 13 is slidably connected to the inside of the slot 16, a mounting groove 27 is provided inside the centrifugal disc 15, the outer wall of the stabilizing block 6 is fixedly connected to the connecting plate 1, and the outer wall of the connecting plate 1 is provided with a driving assembly; the driving assembly includes a first motor 2, the outer wall of the first motor 2 is fixedly connected to the upper surface of the connecting plate 1, the output end of the first motor 2 is fixedly connected to the first transmission roller 3, the outer wall of the first transmission roller 3 is slidably connected to the belt 4, the outer wall of the belt 4 is slidably connected to the second transmission roller 5, and the upper surface of the second transmission roller 5 is fixedly connected to the bottom end of the rotating shaft 7;

[0026] Specifically, the motor 11 drives the block 13 and the bidirectional threaded rod 12 to move, and the block 13 is inserted into the slot 16 to fix the centrifugal disk 15 under the limit of the support plate 14. The centrifugal disk 15 can be replaced according to molds of different sizes. During use, the first motor 2 drives the first transmission roller 3 and the belt 4 to move, and the belt 4 drives the second transmission roller 5 and the rotating shaft 7 to rotate inside the stabilizing block 6, which can have a better limiting effect. During use, the rotating shaft 7 drives the turntable 8 and the connecting disk 10 to rotate. During use, the test tube mold is inserted into the installation groove 27 and centrifugally rotated through the movement of the connecting disk 10. During use, the multiple installation grooves 27 on the centrifugal disk 15 can extract multiple test tubes containing samples at one time.

[0027] Reference Figure 1-Figure 2 The outer wall of the connecting plate 1 is fixedly connected to a housing 17, and the outer wall of the housing 17 is fixedly connected to a hinge 18; the outer wall of the hinge 18 is fixedly connected to a door panel 19, and the inner wall of the door panel 19 is fixedly connected to a door handle 20; the outer wall of the door panel 19 is slidably connected to the inner wall of the housing 17, and the inner wall of the housing 17 is fixedly connected to an outer frame 21;

[0028] Specifically, the door panel 19 is opened through the door handle 20 by limiting the position of the hinge 18, so that the internal structure can be better maintained and repaired during use.

[0029] Reference Figure 4The outer wall of the outer frame 21 is fixedly connected to the second motor 22, and the output end of the second motor 22 is fixedly connected to the first sprocket 23; the outer wall of the first sprocket 23 is meshedly connected to the chain 24, and the outer wall of the chain 24 is meshedly connected to the second sprocket 26; the outer walls of the first sprocket 23 and the second sprocket 26 are both fixedly connected to the fan 25, and the inner wall of the outer frame 21 is fixedly connected to the dustproof net 9;

[0030] Specifically, the second motor 22 drives the first sprocket 23 to rotate, and the first sprocket 23 drives the chain 24 and the second sprocket 26 to rotate on the outer wall of the outer frame 21, and drives the fan 25 to discharge the heat emitted by the electronic components inside the outer shell 17. The dustproof net 9 can prevent dust from entering the interior of the outer shell 17 during use and causing dust pollution to the electronic components inside the outer shell 17.

[0031] Working principle: When the device is needed, the motor 11 drives the block 13 and the bidirectional threaded rod 12 to move, and the block 13 is inserted into the slot 16 to fix the centrifugal disc 15 under the limit of the support plate 14. During use, the centrifugal disc 15 can be replaced according to molds of different sizes to replace the mounting slot 27. During use, the first motor 2 drives the first transmission roller 3 and the belt 4 to move, and the belt 4 drives the second transmission roller 5 and the rotating shaft 7 to rotate inside the stabilizing block 6, which can have a better limiting effect. During use, the rotating shaft 7 drives the rotating disc 8 and the connecting disc 10 to rotate. Rotation, during use, the test tube mold is inserted into the installation groove 27 and centrifugally rotated through the movement of the connecting plate 10. During use, the multiple installation grooves 27 on the centrifugal plate 15 can extract multiple test tubes containing samples at one time. During use, the first sprocket 23 is driven to rotate by the second motor 22 outside the outer frame 21, and the chain 24 and the second sprocket 26 are driven to rotate on the outer wall of the outer frame 21 by the first sprocket 23. The heat emitted by the electronic components inside the housing 17 during use can be discharged by driving the fan 25. During use, the dustproof net 9 can prevent During the process, dust enters the interior of the housing 17 and causes dust pollution to the electronic components inside the housing 17. During use, the door panel 19 is opened through the door handle 20 through the limit of the hinge 18, and the internal structure can be better maintained and repaired during use. During use, the device can not only achieve the centrifugal extraction of recombinant proteins by driving the connecting disk 10 through the turntable 8, but also drive the bidirectional threaded rod 12 through the block 13 to quickly fix the centrifugal disk 15 through the motor 11, and there are multiple installation grooves 27 on the centrifugal disk 15. During use, multiple test tubes containing materials can be placed for The extraction effect is achieved, and the first sprocket 23 and the second sprocket 26 are driven to move by the second motor 22. The fan 25 can discharge the heat dissipated by the internal electronic components during long-term work. The dustproof net 9 can prevent dust from entering the interior and causing pollution to the internal electronic components during use. The centrifugal disk 15 can be quickly installed and disassembled through the block 13. When test tubes of different sizes need to be extracted during use, the block 13 can be slid out from the inside of the slot 16 to replace the centrifugal disk 15. During use, it can better adapt to various extraction molds.

[0032] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A rapid extraction device for recombinant proteins, comprising a stabilizing block (6), characterized in that: The inner wall of the stabilizing block (6) is rotatably connected to a rotating shaft (7), the top end of the rotating shaft (7) is fixedly connected to a rotating disk (8), the upper surface of the rotating disk (8) is fixedly connected to a connecting disk (10), the outer wall of the connecting disk (10) is fixedly connected to a motor (11), the output end of the motor (11) is fixedly connected to a bidirectional threaded rod (12), the outer wall of the bidirectional threaded rod (12) is threadedly connected to a clamping block (13), the inner wall of the connecting disk (10) is fixedly connected to a supporting plate (14), and the inner wall of the connecting disk (10) is fixedly connected to a supporting plate (14). ), the outer wall of the clamping block (13) is slidably connected to the outer wall of the supporting plate (14), the upper surface of the supporting plate (14) is slidably connected to a centrifugal disc (15), a slot (16) is provided inside the centrifugal disc (15), the outer wall of the clamping block (13) is slidably connected to the inside of the slot (16), a mounting groove (27) is provided inside the centrifugal disc (15), the outer wall of the stabilizing block (6) is fixedly connected to a connecting plate (1), and a driving component is provided on the outer wall of the connecting plate (1).

2. The rapid extraction device for recombinant protein according to claim 1, characterized in that: The driving assembly comprises a first motor (2), the outer wall of the first motor (2) is fixedly connected to the upper surface of the connecting plate (1), the output end of the first motor (2) is fixedly connected to a first transmission roller (3), the outer wall of the first transmission roller (3) is slidably connected to a belt (4), the outer wall of the belt (4) is slidably connected to a second transmission roller (5), and the upper surface of the second transmission roller (5) is fixedly connected to the bottom end of the rotating shaft (7).

3. The rapid extraction device for recombinant protein according to claim 2, characterized in that: The outer wall of the connecting plate (1) is fixedly connected to a housing (17), and the outer wall of the housing (17) is fixedly connected to a hinge (18).

4. The rapid extraction device for recombinant protein according to claim 3, characterized in that: The outer wall of the hinge (18) is fixedly connected to a door panel (19), and the inner wall of the door panel (19) is fixedly connected to a door handle (20).

5. The rapid extraction device for recombinant protein according to claim 4, characterized in that: The outer wall of the door panel (19) is slidably connected to the inner wall of the outer shell (17), and the inner wall of the outer shell (17) is fixedly connected to an outer frame (21).

6. The rapid extraction device for recombinant protein according to claim 5, characterized in that: The outer wall of the outer frame (21) is fixedly connected to a second motor (22), and the output end of the second motor (22) is fixedly connected to a first sprocket (23).

7. The rapid extraction device for recombinant protein according to claim 6, characterized in that: The outer wall of the first sprocket (23) is meshedly connected with a chain (24), and the outer wall of the chain (24) is meshedly connected with a second sprocket (26).

8. The rapid extraction device for recombinant protein according to claim 7, characterized in that: The outer walls of the first sprocket (23) and the second sprocket (26) are both fixedly connected to a fan (25), and the inner wall of the outer frame (21) is fixedly connected to a dustproof net (9).

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