Simple protein purification device

Abstract

The utility model provides a simple protein purification device, which relates to the technical field of protein purification, and comprises a peristaltic pump, a first container, a second container and a plurality of chromatography components, the peristaltic pump is provided with a pump head, the pump head is provided with at least one first interface, at least one second interface, a plurality of third interfaces and a plurality of fourth interfaces, the first container is connected with the first interface through a conduit, the second container is connected with the second interface through a conduit, and each chromatography assembly is respectively connected with the third interface and the fourth interface through conduits; the peristaltic pump is used for extracting to-be-purified protein in the first container into the peristaltic pump through the first interface, conveying the to-be-purified protein into the plurality of chromatography assemblies through the plurality of fourth interfaces, purifying to obtain target protein, extracting the target protein into the peristaltic pump through the plurality of third interfaces, and conveying the target protein into the second container through the second interface. According to the utility model, the purification condition of protein is convenient to explore, the protein purification process is accelerated through the multi-channel design formed by the plurality of chromatography assemblies and the plurality of interfaces of the pump head, and the protein purification efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification, in particular to a simple protein purification device. Background Art

[0002] Protein separation and purification has always been an important operation technology in biotechnology applications. Separating and purifying proteins with high activity and high purity has always been the goal pursued by the majority of scientific researchers. At present, there are many commercial protein purifiers and protein purification pre-packed columns, but commercial protein purifiers and pre-packed columns have high procurement costs, high maintenance costs, and high usage requirements. Improper operation can easily damage the instrument or reduce the service life of the column. In addition, protein separation and purification operations have many problems such as complicated preliminary preparations and low timeliness. For example, in the process of purifying a newly expressed protein, it is necessary to explore the purification conditions of the protein. This process requires a lot of manpower and time using commercial protein purifiers, and the work intensity is high.

[0003] The invention and use of simple protein purification devices can greatly improve the efficiency of daily laboratory protein purification condition exploration and small-scale rapid protein purification. After exploring the protein purification conditions, large-scale purification or purification using commercial protein purification instruments can greatly improve the efficiency of protein purification and effectively save the time cost of experimenters. Utility Model Content

[0004] In view of the problem that the protein purification devices in the prior art are too complicated and not convenient for rapid purification of small amounts of protein, resulting in low protein purification efficiency, the utility model provides a simple protein purification device, which is convenient for exploring the purification conditions of the protein, and accelerates the protein purification process and improves the protein purification efficiency by forming a multi-channel design through multiple chromatography components and multiple interfaces of the pump head.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A simple protein purification device, comprising:

[0007] A peristaltic pump having a pump head, wherein the pump head has at least a first interface and a second interface, and a plurality of third interfaces and fourth interfaces;

[0008] A first container connected to the first interface via a conduit;

[0009] a second container connected to the second interface via a conduit;

[0010] A plurality of chromatography components, each of which is connected to the third interface and the fourth interface respectively through a conduit;

[0011] Among them, the peristaltic pump extracts the protein to be purified in the first container into the peristaltic pump through the first interface, and transports it to several chromatography components through several fourth interfaces to purify and obtain the target protein. The peristaltic pump extracts the target protein into the peristaltic pump through several third interfaces, and transports it to the second container through the second interface.

[0012] As described above, the simple protein purification device, further, the chromatography component comprises a nickel affinity chromatography column, and both ends of the nickel affinity chromatography column are respectively connected to the third interface and the fourth interface through conduits.

[0013] The simple protein purification device as described above further comprises a third container, wherein the third container has openings at both ends and a receiving cavity inside, wherein a refrigerant is placed in the receiving cavity, and the nickel affinity chromatography column is placed in the third container with both ends passing through the two openings respectively.

[0014] The simple protein purification device as described above further comprises a support frame, wherein the support frame comprises a support plate, and the support plate is provided with a plurality of fixing holes for embedding the third container.

[0015] As described above, the simple protein purification device, further, the support frame also includes a base plate and a support column, the support plate is slidably connected to the support column, a through hole is provided on the support plate, one end of the support column is fixed to the base plate, the other end of the support column passes through the through hole, the support plate can move along the axial direction of the support column, and the support column is also provided with a fixing ring for fixing the support plate, and the fixing ring is provided with a threaded hole compatible with the bolt.

[0016] The simple protein purification device as described above further comprises a fourth container, in which a refrigerant is placed, and the second container is placed in the fourth container.

[0017] The simple protein purification device as described above, further, the conduit is a hose.

[0018] The simple protein purification device as described above, further, the support frame is made of acrylic.

[0019] Compared with the prior art, the utility model has the following beneficial effects:

[0020] 1. The utility model can realize automatic and rapid protein loading through a peristaltic pump;

[0021] 2. The utility model can realize rapid chromatography of protein samples and improve the efficiency of protein purification through the multi-channel design formed by multiple interfaces of the pump head of the peristaltic pump and multiple chromatography components;

[0022] 3. The utility model can avoid denaturation and inactivation of protein due to overly high temperature during the protein purification process. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 This is a schematic diagram of the structure of a protein purification device according to an embodiment of the utility model;

[0025] In the figure: 1, peristaltic pump; 2, first container; 3, second container; 4, nickel affinity chromatography column; 5, third container; 6, catheter; 7, support plate; 8, fixing hole; 9, bottom plate; 10, support column; 11, fixing ring; 12, bolt; 13, fourth interface; 14, third interface; 15, second interface; 16, first interface. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0027] Example:

[0028] It should be noted that the terms "first", "second", etc. in the specification and claims of the utility model and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the utility model described here can be implemented in an order other than those illustrated or described here. In addition, the terms "including" and "having" in the embodiments of the utility model and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units that are clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0029] It should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0030] In the description of the present invention, "multiple" means at least two, such as two, three, etc., unless otherwise clearly defined. In addition, unless otherwise clearly defined and defined, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0032] The utility model provides a technical solution: a simple protein purification device, which includes a peristaltic pump 1, a first container 2, a second container 3 and a plurality of chromatography components, the peristaltic pump 1 has a pump head, the pump head has at least a first interface 16 and a second interface 15, and a plurality of third interfaces 14 and a fourth interface 13, the first container 2 is connected to the first interface 16 through a conduit 7, the second container 3 is connected to the second interface 15 through the conduit 7, and each chromatography component is respectively connected to the third interface 14 and the fourth interface 13 through the conduit 7; wherein the peristaltic pump 1 extracts the protein to be purified in the first container 2 into the peristaltic pump 1 through the first interface 16, and transports it to the plurality of chromatography components through the plurality of fourth interfaces 13 to purify and obtain the target protein, the peristaltic pump 1 extracts the target protein into the peristaltic pump 1 through the plurality of third interfaces 14, and transports it to the second container 3 through the second interface 15.

[0033] Specifically, compared with the traditional protein purification device, the protein purification device adopts a peristaltic pump 1 with multiple interfaces on the pump head, and is simultaneously connected to multiple chromatography components to form a multi-channel design structure. In this way, when conducting protein purification condition exploration experiments, the speed of protein chromatography can be accelerated and the protein purification efficiency can be improved; even different types of chromatography components can be set to conduct protein purification experiments under different conditions at the same time to improve the adaptability of the device. Among them, the chromatography components include but are not limited to affinity chromatography columns, size exclusion columns, ion exchange chromatography columns, hydrophobic interaction chromatography columns, etc. In addition, the use of a peristaltic pump 1 to transport protein samples has the advantages of high transmission accuracy and simple operation. In this way, the flow rate of the protein can be adjusted by the peristaltic pump 1 to achieve rapid and automatic sampling.

[0034] As an optional embodiment, in some embodiments, the chromatography component includes a nickel affinity chromatography column 4, and both ends of the nickel affinity chromatography column 4 are respectively connected to the third interface 14 and the fourth interface 13 through a conduit 7. Among them, the chromatography component of the protein purification device adopts an affinity chromatography column, preferably, a nickel affinity chromatography column 4 is adopted, and the protein can be purified by binding the protein to the nickel in the chromatography column, and the protein purification effect is better.

[0035] In the above embodiment, further, a third container 5 is included, the two ends of the third container 5 have openings and a receiving cavity (not shown) inside, a refrigerating substance (not shown) is placed in the receiving cavity, and the nickel affinity chromatography column 4 is placed in the third container 5 and the two ends pass through the two openings respectively. Among them, in order to prevent the protein drink from being degraded due to the high ambient temperature during the protein purification process, the nickel affinity chromatography column 4 can be placed in the third container 5, and the refrigerating substance in the receiving cavity of the third container 5 is used to keep the temperature at a relatively low state. The refrigerating substance includes but is not limited to ice cubes, dry ice, etc. In addition, since the protein device is a simple device and is used in daily laboratories, a plastic bottle with the bottom of the bottle cut off and the bottle cap removed can be used as the third container 5, so that the cost can be reduced, waste can be utilized, and it is green and environmentally friendly.

[0036] In the above embodiment, further, a support frame is included, and the support frame includes a support plate 7, and a plurality of fixing holes 8 for clamping the third container 5 are provided on the support plate 7. In order to improve the stability of the placement of the third container 5, a support frame can be provided, and fixing holes 8 that match the shape of the third container 5 can be provided on the support plate 7 of the support frame (for example, if the third container 5 is cylindrical, the fixing holes 8 are circular, and the size of the fixing holes 8 can be adjusted according to the size of the third container 5 to meet the requirements for fixing the third container 5), and the third container 5 can be clamped on the support plate 7, and the fixing structure is stable and easy to operate.

[0037] In the above embodiment, further, the support frame also includes a base plate 9 and a support column 10, the support plate 7 is slidably connected to the support column 10, a through hole is provided on the support plate 7, one end of the support column 10 is fixed on the base plate 9, and the other end of the support column 10 passes through the through hole, the support plate 7 can move along the axial direction of the support column 10, and a fixing ring 11 for fixing the support plate 7 is also provided on the support column 10, and a threaded hole compatible with the bolt 12 is provided on the fixing ring 11.

[0038] Specifically, in order to further improve the adaptability of the protein purification device, the support frame has the function of adjustable height, and a through hole is opened on the support plate 7 for the support column 10 to pass through, so that the support plate 7 can move axially of the support column 10. At the same time, the bottom surface of the support plate 7 abuts against the top surface of the fixing ring 11, and the fixing ring 11 is tightened to the support column 10 by the screwing fit of the bolt 12 and the threaded hole, thereby fixing the support plate 7.

[0039] As an optional embodiment, in some embodiments, a fourth container (not shown) is further included, in which a refrigerant is placed, and the second container 3 is placed in the fourth container. In order to prevent the target protein in the second container 3 from being degraded due to excessively high ambient temperature, the second container 3 can be placed in the fourth container containing the refrigerant, so that the second container 3 is kept at a relatively low temperature. The refrigerant includes but is not limited to ice cubes, dry ice, etc.

[0040] As an optional implementation, in some embodiments, the conduit 7 is a hose. The conduit 7 can be a hose made of rubber or plastic, and the length of the hose can be adjusted according to actual use conditions to meet the test requirements. The hose has good pressure resistance and bending resistance, is not easy to break, and can adapt to various use environments, with high adaptability.

[0041] As an optional implementation, in some embodiments, the support frame is made of acrylic, which has good processing performance and can be manufactured into support frames of different shapes according to requirements, and has good physical properties, which can improve the stability of the support frame.

[0042] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0043] The above embodiments are only for illustrating the technical concept and features of the utility model, and their purpose is to enable ordinary technicians in the field to understand the content of the utility model and implement it accordingly, and they cannot be used to limit the protection scope of the utility model. Any equivalent changes or modifications made according to the essence of the content of the utility model should be included in the protection scope of the utility model.

Claims

1. A simple protein purification device, characterized in that: include: A peristaltic pump having a pump head, wherein the pump head has at least a first interface and a second interface, and a plurality of third interfaces and fourth interfaces; A first container connected to the first interface via a conduit; a second container connected to the second interface via a conduit; A plurality of chromatography components, each of which is connected to the third interface and the fourth interface respectively through a conduit; Among them, the peristaltic pump extracts the protein to be purified in the first container into the peristaltic pump through the first interface, and transports it to several chromatography components through several fourth interfaces to purify and obtain the target protein. The peristaltic pump extracts the target protein into the peristaltic pump through several third interfaces, and transports it to the second container through the second interface.

2. The simple protein purification device according to claim 1, characterized in that: The chromatography component comprises a nickel affinity chromatography column, and two ends of the nickel affinity chromatography column are respectively connected to the third interface and the fourth interface through conduits.

3. The simple protein purification device according to claim 2, characterized in that: It also includes a third container, which has openings at both ends and a containing cavity inside, in which a refrigerant is placed, and the nickel affinity chromatography column is placed in the third container with both ends passing through the two openings respectively.

4. The simple protein purification device according to claim 3, characterized in that: It also includes a support frame, which includes a support plate. The support plate is provided with a plurality of fixing holes for locking the third container.

5. The simple protein purification device according to claim 4, characterized in that: The support frame also includes a base plate and a support column, the support plate is slidably connected to the support column, a through hole is provided on the support plate, one end of the support column is fixed to the base plate, the other end of the support column passes through the through hole, the support plate can move along the axial direction of the support column, and a fixing ring for fixing the support plate is also provided on the support column, and a threaded hole compatible with the bolt is provided on the fixing ring.

6. The simple protein purification device according to claim 1, characterized in that: It also includes a fourth container, in which a refrigerant is placed, and the second container is placed in the fourth container.

7. The simple protein purification device according to claim 1, characterized in that: The conduit is a hose.

8. The simple protein purification device according to claim 4, characterized in that: The support frame is made of acrylic.

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