Cell filtering device

Abstract

The utility model provides a cell filtering device, and relates to the field of medical treatment. The cell filtering device comprises a support, a double-end needle, a filtering container and a negative pressure type container, the double-end needle, the filtering container and the negative pressure type container are arranged on the support in the first direction, the negative pressure type container is communicated with the filtering container through the double-end needle, the filtering container is provided with a filtering membrane, and the double-end needle comprises a first end and a second end. The first end is arranged in the filtering container in a penetrating mode, and the second end is arranged in the negative pressure type container in a penetrating mode. The cells can be automatically driven to move towards the negative pressure type container through negative pressure in the negative pressure type container, cell screening is achieved through the filtering membrane of the filtering container, the cell filtering device is simple and reliable, the preparation cost of the cell filtering device is greatly reduced, and medical staff can conveniently use the cell filtering device.

Description

Technical Field

[0001] The present application relates to the medical field, and in particular, to a cell filtration device. Background Art

[0002] Circulating tumor cells (CTCs) refer to tumor cells that are released from solid tumors or metastatic lesions into the peripheral blood circulation spontaneously or due to diagnostic and therapeutic procedures. They are an important cause of postoperative recurrence and distant metastasis in patients with malignant tumors.

[0003] In recent years, with the continuous improvement of detection technology, CTCs detection, as a new type of non-invasive diagnostic tool, has become a hot topic in clinical research for its application value in early detection of postoperative recurrence and distant metastasis, evaluation of efficacy and prognosis, etc. However, due to the extremely small number of CTCs in peripheral blood, it is usually necessary to find only a few tumor cells among about 100 million white blood cells and 50 billion red blood cells. Therefore, in order to improve the detection rate of CTCs, it is usually necessary to enrich CTCs before detection, that is, to separate CTCs from blood or other samples.

[0004] At present, the enrichment methods of CTCs are mainly divided into immunomagnetic separation method and enrichment method based on the physical properties of CTCs according to their principles. Among them, the enrichment method based on the physical properties of CTCs mainly includes density gradient centrifugation method and filtration method.

[0005] The usual filtration method is the separation method based on tumor cell size (full name in English is isolation by size of epithelial tumor cells, abbreviated in English as ISET), which mainly separates tumor cells based on the size difference between tumor cells and normal cells. The cell morphology separated by this physical method is intact, and the various antigens or molecular markers on the surface are not destroyed, which does not affect the characteristics of the cells, providing good conditions for subsequent detection.

[0006] Existing cell filtration devices include pumps, valves, connecting pipes, waste liquid collection, power supplies, control circuits and other components. The filtration devices are large in size and take up a lot of space. In addition, the filtration devices are complicated to operate and have high preparation costs, which is not conducive to the promotion and use of medical technology. Utility Model Content

[0007] The purpose of the present application is to provide a cell filtration device that can simplify the structure of the cell filtration device, facilitate carrying and operation by medical personnel, reduce the preparation cost of the cell filtration device, and facilitate the promotion and use of medical technology.

[0008] In the first aspect, the utility model provides a cell filtration device, which is used to screen first cells with a preset size. The cell filtration device includes a bracket and a double-ended needle installed on the bracket along a first direction, a filtration container and a negative pressure container. The negative pressure container is connected to the filtration container through the double-ended needle. The filtration container is provided with a filtration membrane. The double-ended needle includes a first end and a second end. The first end is penetrated through the filtration container, and the second end is penetrated through the negative pressure container.

[0009] In an optional embodiment, the bracket includes a main body and a frame fixedly connected to each other, the main body is provided with a through hole for the double-ended needle to pass through, the negative pressure container is mounted on the main body, and the filter container is mounted on the frame.

[0010] In an optional embodiment, the main body is further provided with a mounting groove, the mounting groove is communicated with the through hole, and the groove wall of the mounting groove is adapted to the outer periphery of the negative pressure container.

[0011] In an optional embodiment, the frame is provided with a receiving groove and a limiting portion, the limiting portion is arranged on a groove wall of the receiving groove, and the groove wall of the receiving groove is adapted to the outer periphery of the filter container.

[0012] In an optional embodiment, the filter container includes a tube body and a rubber plug sealed to the tube body, the rubber plug is matched with the limiting portion, the first end is penetrated by the rubber plug, and the outer periphery of the tube body is matched with the groove wall of the containing groove.

[0013] In an optional embodiment, the cell filtration device also includes a needle withdrawal spring and a limiting member, a limiting space is defined between the limiting member and the bracket, the needle withdrawal spring is installed in the limiting space, and the needle withdrawal spring is also provided with a mounting hole, and the double-headed needle is passed through the mounting hole.

[0014] In an optional embodiment, the needle withdrawal spring includes an abutment portion and a plurality of elastic legs, the abutment portion is provided with the mounting hole, the plurality of elastic legs are fixedly connected to the abutment portion, and each of the elastic legs is supported on the bracket.

[0015] In an optional embodiment, the limiting member is configured as a plurality of screws, and a limiting space is defined between the plurality of screws and the bracket.

[0016] In an optional embodiment, the first end is provided with a liquid inlet, which is communicated with the filter container, and the second end is provided with a liquid outlet, which is communicated with the negative pressure container.

[0017] In an optional embodiment, the cell filtration device further comprises an elastic sleeve, wherein the elastic sleeve is sleeved on the double-ended needle and is suitable for shielding or exposing the liquid outlet.

[0018] Compared with the prior art, the beneficial effects of this application are:

[0019] The present application improves the flexibility of use of the cell filtration device by using a bracket as a fulcrum for carrying and using, and the filtration container and the negative pressure container of the present application are connected by a double-ended needle. The negative pressure in the negative pressure container will automatically drive the cells to move toward the negative pressure container, and the filter membrane of the filtration container is used to achieve cell screening, which is simple and reliable, greatly reduces the preparation cost of the cell filtration device, and is convenient for medical personnel to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 An exploded schematic diagram of a cell filtration device in some embodiments is shown;

[0022] Figure 2 Shown is a schematic diagram of the assembly of a cell filtration device in some embodiments;

[0023] Figure 3 Schematic cross-sectional views of the stents in some embodiments are shown;

[0024] Figure 4 Shows a schematic diagram of the structure of a double-ended needle in some embodiments;

[0025] Figure 5 It shows a schematic structural diagram of a needle withdrawal spring in some embodiments;

[0026] Figure 6 Schematic cross-sectional views of cell filtration devices in some embodiments are shown.

[0027] Description of main component symbols:

[0028] 100-bracket; 110-main body; 111-installation part; 112-through hole; 113-installation slot; 114-visible window; 120-frame; 121-accommodation slot; 122-limiting part; 200-double-ended needle; 210-liquid inlet; 220-liquid outlet; 300-filter container; 310-tube body; 320-rubber plug; 400-negative pressure container; 410-silicone plug; 500-needle withdrawal spring; 510-abutment part; 511-installation hole; 520-elastic support leg; 600-limiting piece; 700-elastic sleeve. DETAILED DESCRIPTION

[0029] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

[0030] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0031] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0032] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0033] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0034] Embodiment 1

[0035] This embodiment provides a cell filtration device for screening first cells having a first size. Exemplarily, this embodiment can set the first cells as circulating tumor cells. This embodiment is suitable for screening circulating tumor cells in peripheral blood. The size of circulating tumor cells is larger than the size of other normal cells in peripheral blood.

[0036] See also Figure 1 and Figure 2 The cell filtration device comprises a support 100 and a double-ended needle 200, a filtration container 300 and a negative pressure container 400 mounted on the support 100 along a first direction. In this embodiment, the first direction is a vertical direction, in which the filtration container 300 is located at the top, the double-ended needle 200 is located in the middle, and the negative pressure container 400 is located at the bottom.

[0037] See also Figure 1 and Figure 3 The support 100 serves as a fulcrum for carrying and using, thereby improving the flexibility of using the cell filtration device. The support 100 includes a body 110 and a frame 120 which are fixedly connected to each other.

[0038] The body 110 is provided with a mounting portion 111, a through hole 112, a mounting groove 113 and a visible window 114. The through hole 112 penetrates the mounting portion 111, and the mounting groove 113 is connected to the through hole 112. In this embodiment, the mounting groove 113 can be set as a circular groove, and the visible window 114 is set on the groove wall of the mounting groove 113. The implementation methods of the visible window 114 include but are not limited to the following two implementation methods:

[0039] The first implementation is to directly open the visible window 114 on the wall of the installation groove 113. The second implementation is to set the visible window 114 made of transparent material on the wall of the installation groove 113.

[0040] The frame body 120 is provided with a receiving groove 121 and a limiting portion 122 . The limiting portion 122 is disposed on the groove wall of the receiving groove 121 . In this embodiment, the receiving groove 121 can be set as a through groove.

[0041] See also Figure 1 , Figure 3 and Figure 4 The double-ended needle 200 is inserted into the through hole 112 and fixedly connected to the main body 110. In this embodiment, the double-ended needle 200 includes a first end and a second end. The first end is inserted into the filter container 300 and is provided with a liquid inlet 210, and the liquid inlet 210 is communicated with the filter container 300. The second end is inserted into the negative pressure container 400 and is provided with a liquid outlet 220, and the liquid outlet 220 is communicated with the negative pressure container 400, that is, the negative pressure container 400 is communicated with the filter container 300 through the double-ended needle 200, and the liquid inlet 210 and the liquid outlet 220 are respectively located on the sides of the double-ended needle 200.

[0042] This embodiment realizes the connection between the negative pressure container 400 and the filtering container 300 through the double-ended needle 200, which has the advantages of simplifying the pipeline, improving reliability, reducing the preparation cost and use cost, and being simple to operate.

[0043] Please continue reading Figure 1 The filter container 300 is installed on the frame 120. Specifically, the outer periphery of the filter container 300 is matched with the groove wall of the containing groove 121. In actual use, the filter container 300 includes a tube body 310 and a plug 320 sealed with the tube body 310. The plug 320 is matched with the limiting portion 122, and the outer periphery of the tube body 310 is matched with the groove wall of the containing groove 121.

[0044] After the medical staff installs the filter container 300 to the frame 120, the first end of the double-ended needle 200 pierces the rubber plug 320 and passes through the rubber plug 320. At this time, the filter container 300 is connected to the double-ended needle 200, but due to the lack of pressure difference, the peripheral blood remains in the filter container 300 and does not flow into the double-ended needle 200.

[0045] The filter container 300 is provided with a filter membrane, which is laid flat in the filter container 300, and after the pressure difference is generated, the filter container 300 can filter all peripheral blood, thereby retaining the circulating tumor cells in the peripheral blood above the filter membrane, that is, the circulating tumor cells are retained in the filter container 300, and other normal cells are filtered into the double-ended needle 200, thereby completing the screening of circulating tumor cells. It should be understood that the filter membrane is provided with honeycomb filter holes, and the pore size of the filter holes is smaller than the size of the circulating tumor cells.

[0046] See also Figure 1 and Figure 3 The negative pressure container 400 is installed on the main body 110. Specifically, the outer circumference of the negative pressure container 400 is adapted to the groove wall of the mounting groove 113. Corresponding to the setting of the mounting groove 113 as a circular groove, the outer circumference of the negative pressure container 400 is cylindrical.

[0047] The negative pressure container 400 refers to a container in a negative pressure state. The preset negative pressure in the negative pressure container 400 can draw the normal cells in the filtration container 300 into the negative pressure container 400 .

[0048] In actual use, after the medical staff installs the negative pressure container 400 to the main body 110, the second end of the double-ended needle 200 pierces the silicone plug 410 of the negative pressure container 400 and passes through the negative pressure container 400. At this time, the negative pressure container 400 is connected to the double-ended needle 200, and a pressure difference is generated between the negative pressure in the negative pressure container 400 and the pressure in the filter container 300. Normal cells in the peripheral blood flow to the negative pressure container 400 through the double-ended needle 200 under the filtering action of the filter membrane, realizing the function of radial separation and enrichment, thereby achieving the purpose of sample separation.

[0049] After the separation is completed, the normal cells in the negative pressure container 400 are defined as waste liquid. The negative pressure container 400 can store the waste liquid and transfer it to the next medical treatment process, effectively avoiding the problem of biological contamination.

[0050] In this embodiment, the negative pressure container 400 may be a blood collection tube or other container with a preset negative pressure state.

[0051] Since the present embodiment is provided with a visible window 114, when the negative pressure container 400 is connected to the double-ended needle 200, the flow of normal cells can be visually observed and the filtration condition can be monitored in time. When medical personnel observe that the flow stops, indicating that the filtration is finished, the negative pressure container 400 can be pulled out at this time.

[0052] See also Figure 1 and Figure 5 The cell filtering device also includes a needle withdrawal spring piece 500 and a limiting member 600. A limiting space is defined between the limiting member 600 and the bracket 100. The needle withdrawal spring piece 500 is installed in the limiting space, and the needle withdrawal spring piece 500 is also provided with a mounting hole 511, and the double-headed needle 200 is passed through the mounting hole 511.

[0053] The needle withdrawal spring 500 includes a contact portion 510 and a plurality of elastic legs 520 . The contact portion 510 is provided with a mounting hole 511 . The plurality of elastic legs 520 are fixedly connected to the contact portion 510 , and each elastic leg 520 is supported on the mounting portion 111 of the bracket 100 .

[0054] The limiting member 600 is configured as a plurality of screws, and a limiting space is defined between the plurality of screws and the bracket 100. Specifically, in this embodiment, the number of screws can be set to two, and both screws are disposed above the mounting portion 111.

[0055] Please continue reading Figure 1When installing the filter container 300, the filter container 300, under the action of the upper groove wall of the accommodating groove 121, presses against the needle withdrawal spring piece 500 and causes the needle withdrawal spring piece 500 to deform. When removing the filter container 300, the medical staff pulls out the filter container 300, the filter container 300 loses the pressing effect of the groove wall, and the needle withdrawal spring piece 500 resumes its deformation, thereby ejecting the filter container 300.

[0056] See also Figure 1 and Figure 4 The cell filtering device further comprises an elastic sleeve 700 , which is sleeved on the double-ended needle 200 and is suitable for covering or exposing the liquid outlet 220 .

[0057] Before the negative pressure container 400 is installed on the main body 110, the elastic sleeve 700 covers the liquid outlet 220. On the one hand, it can prevent peripheral blood from flowing out of the liquid outlet 220 and causing biological contamination. On the other hand, the elastic sleeve 700 can protect the medical hygiene of the liquid outlet 220 and prevent the double-ended needle 200 from being contaminated by the external environment.

[0058] When the negative pressure container 400 is installed, the negative pressure container 400 pushes the elastic sleeve 700 upward to expose the liquid outlet 220, so that the negative pressure container 400 can be connected with the double-ended needle 200 after installation. In this embodiment, the negative pressure container 400 and the filter container 300 are both disposable consumables. After one use, the negative pressure container 400 and the filter container 300 are both treated as medical waste.

[0059] Please continue reading Figure 1 , Figure 2 and Figure 6 Based on the above content, this embodiment further explains the use principle of the cell filtration device as follows:

[0060] S100. Pre-install the elastic sleeve 700 on the second end of the double-ended needle 200, and

[0061] 700 shields the liquid outlet 220 .

[0062] S200 . Pass the double-ended needle 200 through the through hole 112 and fix the double-ended needle 200 to the mounting portion 111 of the bracket 100 , so that the first end is located above the mounting portion 111 and the second end is located below the mounting portion 111 .

[0063] S300. Place the needle withdrawal spring piece 500 on the mounting portion 111, and install two stoppers 600 to ensure that the needle withdrawal spring piece 500 can be elastically deformed and will not fall off.

[0064] S400. After the filter container 300 is installed on the frame 120, the first end of the double-ended needle 200 pierces the rubber plug 320 and passes through the rubber plug 320, and the filter container 300 presses against the needle withdrawal spring piece 500 under the action of the upper groove wall of the accommodating groove 121 and causes the needle withdrawal spring piece 500 to deform.

[0065] S500. After the negative pressure container 400 is installed on the main body 110, the second end of the double-ended needle 200 pierces the silicone plug 410 of the negative pressure container 400 and penetrates the negative pressure container 400, and normal cells gradually flow from the filtering device to the negative pressure container 400.

[0066] S600. After the flow stops, the negative pressure container 400 is pulled out, and the ring tumor cells are retained in the filter container 300.

[0067] S700. Pull out the filter container 300. The filter container 300 loses the supporting effect of the groove wall, and the needle withdrawal spring 500 recovers its deformation, thereby ejecting the filter container 300.

[0068] This embodiment utilizes the negative pressure in the negative pressure container 400 to achieve the cell screening function of peripheral blood, which is simple and reliable, greatly reduces the preparation cost of the cell filtering device, and is convenient for medical personnel to use.

[0069] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0070] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A cell filtering device for screening first cells having a preset size, characterized in that: It includes a bracket and a double-ended needle installed on the bracket along a first direction, a filter container and a negative pressure container, the negative pressure container is connected to the filter container through the double-ended needle, the filter container is provided with a filter membrane, the double-ended needle includes a first end and a second end, the first end is penetrated through the filter container, and the second end is penetrated through the negative pressure container.

2. The cell filtration device according to claim 1, characterized in that The bracket comprises a main body and a frame which are fixedly connected to each other, the main body is provided with a through hole for the double-ended needle to pass through, the negative pressure container is installed on the main body, and the filter container is installed on the frame.

3. The cell filtration device according to claim 2, characterized in that The main body is further provided with a mounting groove, the mounting groove is communicated with the through hole, and the groove wall of the mounting groove is adapted to the outer periphery of the negative pressure container.

4. The cell filtration device according to claim 2, characterized in that The frame is provided with a receiving groove and a limiting portion, wherein the limiting portion is arranged on a groove wall of the receiving groove, and the groove wall of the receiving groove is matched with the outer periphery of the filtering container.

5. The cell filtration device according to claim 4, characterized in that The filter container includes a tube body and a rubber plug sealed with the tube body, the rubber plug is matched with the limiting portion, the first end is penetrated by the rubber plug, and the outer periphery of the tube body is matched with the groove wall of the containing groove.

6. The cell filtration device according to any one of claims 1 to 4, characterized in that: It also includes a needle withdrawal spring and a limiting member, wherein a limiting space is defined between the limiting member and the bracket, the needle withdrawal spring is installed in the limiting space, and the needle withdrawal spring is also provided with a mounting hole, and the double-ended needle is passed through the mounting hole.

7. The cell filtration device according to claim 6, characterized in that The needle withdrawal spring comprises an abutment portion and a plurality of elastic legs, the abutment portion is provided with the mounting hole, the plurality of elastic legs are fixedly connected to the abutment portion, and each of the elastic legs is supported on the bracket.

8. The cell filtration device according to claim 7, characterized in that The limiting member is configured as a plurality of screws, and a limiting space is defined between the plurality of screws and the bracket.

9. The cell filtration device according to any one of claims 1 to 4, characterized in that: The first end is provided with a liquid inlet, which is communicated with the filter container, and the second end is provided with a liquid outlet, which is communicated with the negative pressure container.

10. The cell filtration device according to claim 9, characterized in that It also includes an elastic sleeve, which is sleeved on the double-ended needle and is suitable for covering or exposing the liquid outlet.

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