Device for cell-free expression optimization

Abstract

The utility model discloses a device for cell-free expression optimization, which belongs to the technical field of biological detection instruments and comprises an EP tube, and a sensor component used for measuring temperature and / or measuring concentration of biomolecules in a reaction system is mounted at the upper end of the EP tube. The sensor assembly comprises a sensor device and a sealing device, the EP pipe is connected with the sensor device, and the sensor device is connected with the sealing device; a sealing membrane assembly convenient for experiment recording is mounted on the EP tube; and an inert device for assisting oscillation and uniform mixing is mounted on the EP pipe. Through the mode, the temperature difference between the metal bath and the reaction liquid in the test tube is eliminated by measuring the experiment temperature through the device, the stability of cell-free expression is improved, and meanwhile, the reaction device is sealed, so that pollution caused by long-time contact between an internal solution and external air during next-step operation is avoided; the recorded part is protected through the device, and the situation that the surface of the recorded part is polluted or rubbed to be damaged and cannot be recorded is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection instruments, and in particular to a device for optimizing cell-free expression. Background Art

[0002] With the development of science and technology, the country has invested more and more in research in the biological field, so more and more experimental consumables are needed. Centrifuge tubes are an indispensable and important product in biological experiments. They can seal experimental samples to avoid external contamination.

[0003] For example, Chinese utility model patent CN216678287U discloses a centrifuge tube, in which a tube cover is detachably fixed at the tube mouth to seal the entire tube cavity from the outside world, and the corresponding tube wall is flat to provide a good reaction environment for the reaction substances during the culture process.

[0004] However, it still has the following disadvantages. Some experiments require real-time control of the temperature of the experimental reaction to prevent the substances involved in the reaction from losing their activity due to high temperature, or the transcription and translation reaction efficiency is low under low temperature conditions. At the same time, the device may not be sufficiently shaken during the shaking process, resulting in the material not being mixed evenly.

[0005] Based on this, the utility model designs a device for cell-free expression optimization to solve the above problems. Utility Model Content

[0006] In view of the above-mentioned shortcomings of the prior art, the utility model provides a device for optimizing cell-free expression.

[0007] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A device for cell-free expression optimization, comprising an EP tube, wherein a sensor component for measuring temperature and / or the concentration of biomolecules in a reaction system is installed at the upper end of the EP tube;

[0009] The sensor assembly includes a sensor device and a sealing device, the EP tube is connected to the sensor device, and the sensor device is connected to the sealing device;

[0010] The EP tube is equipped with a sealing membrane assembly for easy experimental recording;

[0011] The EP tube is equipped with an inert device for assisting oscillation and mixing.

[0012] Furthermore, the sensor device includes a sealing cover, a slot, a petal-shaped sealing ring and a temperature probe.

[0013] Furthermore, the upper end of the EP tube is slidably connected with a sealing cover, which is connected to the sealing device. A slot is provided in the middle of the upper end of the sealing cover, a petal-type sealing ring is fixedly connected to the bottom of the slot, a temperature probe is slidably connected in the slot, and the temperature probe is in close contact with the petal-type sealing ring.

[0014] Furthermore, the sealing device includes a sealing cover 2, a connecting rod and a protrusion, the left end of the sealing cover 1 is fixedly connected to one end of the connecting rod, the other end of the connecting rod is fixedly connected to the sealing cover 2, the outer wall of the sealing cover 2 is fixedly connected to the end away from the connecting rod with the protrusion, and the inner wall of the sealing cover 2 is snap-connected to the sealing cover 1.

[0015] Furthermore, the petal-shaped sealing ring is a petal-shaped sealing ring made of rubber material.

[0016] Furthermore, the sealing film assembly includes a sealing film, a pull ring cap and a recording sticker. The outer wall of the front end of the EP tube is fixedly connected to the recording sticker, the outer wall of the upper end of the EP tube is contacted with the sealing film, the sealing film is covered on the recording sticker, and the pull ring cap is fixedly connected to the middle part of the front end of the sealing film.

[0017] Furthermore, the connection between the two ends of the sealing film is in a zigzag shape.

[0018] Furthermore, the inert device includes zirconia beads, and a plurality of zirconia beads are placed at the bottom of the EP tube.

[0019] The utility model has the following technical effects:

[0020] 1. The utility model seals the EP tube tightly with a sealing cover 1 of the sensor device of the sensor assembly, drives the sealing cover 2 to rotate along the direction of the connecting rod by pulling the convex block of the sealing device, slides the temperature probe into the EP tube along the slot, and sticks to the temperature probe with a petal-shaped sealing ring. The temperature during the experiment is measured by the device, thereby eliminating the temperature difference between the metal bath and the reaction solution in the test tube, improving the stability of the cell-free expression, and at the same time, the sealing of the reaction device prevents the internal solution from being contaminated by long-term contact with the outside air during the next operation.

[0021] 2. The utility model drives the sealing film to rotate along the outer wall of the EP tube by pulling the pull ring cap of the sealing film assembly, records the required contents of the experiment by the recording sticker, and protects the recorded part by the device to prevent its surface from being damaged by pollution or friction and unable to record. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0023] Figure 1 A three-dimensional device for optimizing cell-free expression according to the utility model Figure 1 ;

[0024] Figure 2 This is a front view of a device for cell-free expression optimization according to the utility model;

[0025] Figure 3 This is a left view of a device for cell-free expression optimization according to the utility model;

[0026] Figure 4 For along Figure 3 AA direction cross-sectional view;

[0027] Figure 5 For along Figure 2 BB direction cross-sectional view;

[0028] Figure 6 for Figure 5 Enlarged view of point C in the middle.

[0029] The numbers in the figure represent:

[0030] 1. EP tube 2. Sensor assembly 21. Sensor device 22. Sealing device 211. Sealing cover 1 212. Slot 213. Petal-type sealing ring 214. Temperature probe 221. Sealing cover 2 222. Connecting rod 223. Bump 3. Sealing film assembly 31. Sealing film 32. Pull ring cap 33. Recording sticker 4. Inert device 41. Zirconia beads. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0032] The utility model is further described below in conjunction with embodiments.

[0033] The terms “left”, “right”, “front”, “back”, “up” and “down” mentioned in the following description are oriented in the viewing direction of the front view.

[0034] Example 1

[0035] Please refer to the instruction manual Figure 1-6 , a device for cell-free expression optimization, comprising an EP tube 1;

[0036] The upper end of the EP tube 1 is provided with a sensor assembly 2 for measuring temperature and / or for measuring the concentration of biomolecules in the reaction system;

[0037] The sensor assembly 2 includes a sensor device 21 and a sealing device 22, the EP tube 1 is connected to the sensor device 21, and the sensor device 21 is connected to the sealing device 22;

[0038] The sensor device 21 includes a sealing cover 211, a slot 212, a petal-shaped sealing ring 213 and a temperature probe 214. The upper end of the EP tube 1 is fitted and slidably connected with the sealing cover 211, and the sealing cover 211 is connected to the sealing device 22. A slot 212 is provided in the middle of the upper end of the sealing cover 211, and a petal-shaped sealing ring 213 is fixedly connected to the bottom of the slot 212. The temperature probe 214 is slidably connected in the slot 212, and the temperature probe 214 is fitted and contacted with the petal-shaped sealing ring 213;

[0039] The sealing device 22 comprises a sealing cover 221, a connecting rod 222 and a protrusion 223. The left end of the sealing cover 211 is fixedly connected to one end of the connecting rod 222, the other end of the connecting rod 222 is fixedly connected to the sealing cover 221, the outer wall of the sealing cover 221 is fixedly connected to the protrusion 223 at one end away from the connecting rod 222, and the inner wall of the sealing cover 221 is snap-connected to the sealing cover 1 211;

[0040] Preferably, the petal-shaped sealing ring 213 is a petal-shaped sealing ring 213 made of rubber material;

[0041] The petal-shaped sealing ring 213 made of the rubber material can increase the friction with the outer wall of the temperature probe 214 to fix it;

[0042] In the embodiment of the utility model, when the device starts to measure the temperature in the experiment, the EP tube 1 is tightly sealed by the sealing cover 1 211 of the sensor device 21 of the sensor assembly 2, and the sealing cover 221 is driven to rotate along the direction of the connecting rod 222 by pulling the protrusion 223 of the sealing device 22, and the temperature probe 214 is slid into the EP tube 1 along the slot 212, and the petal-shaped sealing ring 213 is used to close the temperature probe 214. The temperature during the experiment is measured by the device, which eliminates the temperature difference between the metal bath and the reaction solution in the test tube, improves the stability of the cell-free expression, and at the same time, the sealing of the reaction device prevents the internal solution from being contaminated by long-term contact with the outside air during the next operation.

[0043] Example 2

[0044] Please refer to the instruction manual Figure 5-6 , the EP tube 1 is installed with a sealing membrane assembly 3 for easy experimental recording;

[0045] The sealing film assembly 3 includes a sealing film 31, a pull ring cap 32 and a recording sticker 33. The front end outer wall of the EP tube 1 is fixedly connected with the recording sticker 33. The upper end outer wall of the EP tube 1 is in contact with the sealing film 31. The sealing film 31 is coated on the recording sticker 33. The pull ring cap 32 is fixedly connected to the middle of the front end of the sealing film 31.

[0046] Preferably, the connection between the head and tail ends of the sealing film 31 is in a zigzag shape;

[0047] The sawtooth connection facilitates tearing off the sealing film 31 to use the recording sticker 33 inside;

[0048] In the embodiment of the utility model, when the device is ready to record, the sealing film 31 is driven to rotate along the outer wall of the EP tube 1 by pulling the pull ring cap 32 of the sealing film assembly 3, and the required content of the experiment is recorded by the recording sticker 33. The recorded part is protected by the device to prevent its surface from being contaminated or rubbed, which may cause it to be damaged and unable to record.

[0049] The EP tube 1 is provided with an inert device 4 for assisting oscillation and mixing;

[0050] The inert device 4 includes zirconia beads 41, and a plurality of zirconia beads 41 are placed at the bottom of the EP tube 1;

[0051] In the embodiment of the utility model, when the device starts the laboratory, by adding a proper amount of zirconium oxide beads 41 of the inert device 4 into the EP tube 1, the disadvantage of poor horizontal oscillation and mixing effect of the solution in the EP tube is overcome, and the level of cell-free expression is improved.

[0052] The sensor assembly may include one or a combination of a temperature sensor and a biosensor, which are used to measure the temperature and the concentration of biomolecules in the reaction system respectively, so as to achieve real-time monitoring of the corresponding indicators.

[0053] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for optimizing cell-free expression, comprising an EP tube (1), characterized in that: The upper end of the EP tube (1) is provided with a sensor component (2) for measuring temperature and / or for measuring the concentration of biomolecules in the reaction system; The sensor assembly (2) comprises a sensor device (21) and a sealing device (22), the EP tube (1) is connected to the sensor device (21), and the sensor device (21) is connected to the sealing device (22); The EP tube (1) is provided with a sealing membrane assembly (3) for facilitating experimental recording; The EP tube (1) is equipped with an inert device (4) for assisting oscillation and mixing.

2. The device for cell-free expression optimization according to claim 1, characterized in that: The sensor device (21) comprises a sealing cover (211), a slot (212), a petal-shaped sealing ring (213) and a temperature probe (214).

3. The device for cell-free expression optimization according to claim 2, characterized in that: The upper end of the EP tube (1) is fitted and slidably connected with a sealing cover (211), the sealing cover (211) is connected to a sealing device (22), a slot (212) is provided in the middle of the upper end of the sealing cover (211), a petal-shaped sealing ring (213) is fixedly connected to the bottom of the slot (212), a temperature probe (214) is slidably connected in the slot (212), and the temperature probe (214) is fitted and contacted with the petal-shaped sealing ring (213).

4. The device for cell-free expression optimization according to claim 3, characterized in that: The sealing device (22) comprises a second sealing cover (221), a connecting rod (222) and a protrusion (223); the left end of the first sealing cover (211) is fixedly connected to one end of the connecting rod (222); the other end of the connecting rod (222) is fixedly connected to the second sealing cover (221); the end of the outer wall of the second sealing cover (221) away from the connecting rod (222) is fixedly connected to the protrusion (223); and the inner wall of the second sealing cover (221) is snap-connected to the first sealing cover (211).

5. The device for cell-free expression optimization according to claim 4, characterized in that: The petal-shaped sealing ring (213) is a petal-shaped sealing ring (213) made of rubber material.

6. The device for cell-free expression optimization according to claim 5, characterized in that: The sealing film assembly (3) comprises a sealing film (31), a pull ring cap (32) and a recording sticker (33); the front end outer wall of the EP tube (1) is fixedly connected to the recording sticker (33); the upper end outer wall of the EP tube (1) is in contact with the sealing film (31); the sealing film (31) is coated on the recording sticker (33); and the pull ring cap (32) is fixedly connected to the middle part of the front end of the sealing film (31).

7. The device for cell-free expression optimization according to claim 6, characterized in that: The connection points at both ends of the sealing film (31) are connected in a zigzag shape.

8. The device for cell-free expression optimization according to claim 7, characterized in that: The inert device (4) comprises zirconium oxide beads (41), and a plurality of zirconium oxide beads (41) are placed at the bottom of the EP tube (1).

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