Cell culture device

Abstract

The utility model relates to a cell culture device. The cell culture device comprises a shell, rollers, a partition plate, a filter box and an air blower, the bottom of the inner wall of the shell is connected with the partition plate. An inclined culture structure for inclined shaking of cells is arranged in the shell; a discharging structure used for automatic discharging is arranged on the side face of the partition plate. A pushing structure for keeping the interior of the shell sealed is arranged at the bottom of the inner wall of the shell; the inner wall side surface of the shell is connected with a blower; an air inlet window is arranged on the side surface of the shell; according to the device, the efficiency and the accuracy of cell culture can be effectively improved, the loose structure of cells can be effectively improved in an inclined shaking manner, so that the problems of cell aggregation and the like are reduced, discrete cells can be conveniently counted, the counting difficulty of workers is reduced, and the counting efficiency of the workers is improved. Meanwhile, the device is combined with a rotating door through a sucking disc mode, so that the damage to the internal environment of the shell in the sampling process of the culture dish can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of culture devices, in particular to a cell culture device. Background Art

[0002] Currently, there are a large number of cell culture devices on the market, especially in application scenarios such as large-scale tissue engineering and stem cell culture that require multiple culture tasks to be performed simultaneously. Each cell culture device set up separately will take up a lot of space.

[0003] The Chinese patent with publication number CN219972324U discloses a cell continuous culture device, including an incubator, a gas manufacturing device, a shelf, a culture dish, a water supply device, a carbon dioxide concentration meter, a bracket and a covering device, wherein the gas manufacturing device is arranged at the lower part of the incubator, the shelf is arranged on the inner wall of the incubator, the culture dish is arranged on the shelf, the water supply device is arranged on the outer wall of the incubator and is located above the culture dish, the carbon dioxide concentration meter is arranged on the inner wall of the incubator, the bracket is arranged on the outer wall of the incubator and is located at the upper part, and the covering device is arranged on the bracket and can cover the upper end surface of the incubator. The utility model belongs to the field of biological cell culture technology, specifically refers to a cell continuous culture device that is convenient for accurately controlling carbon dioxide concentration, convenient for applying water, and capable of light.

[0004] However, the above-mentioned publicly disclosed scheme has the following shortcomings: in actual use, it is difficult to count the number of cells during the cell culture process. The fixed culture structure of the above-mentioned device is prone to produce cell clusters, which increases the difficulty of counting. At the same time, the above-mentioned device is prone to damage the internal dust-free environment during the sampling process, thereby posing a risk of introducing miscellaneous bacteria. Utility Model Content

[0005] The purpose of the utility model is to address the technical problems existing in the background technology. The utility model proposes a cell culture device. The utility model can effectively improve the efficiency and accuracy of cell culture through the device, and can effectively improve the loose structure of cells by tilting and shaking, thereby reducing problems such as cell clustering, and discrete cells can be easy to count, thereby reducing the counting difficulty of staff. At the same time, the device can reduce the damage to the internal environment of the shell during the sampling process of the culture dish through the combination of the suction cup and the rotating door, thereby reducing the external environment air from entering the shell of the device during the automatic sampling process of the device, and ensuring the purity of the culture dish.

[0006] The utility model provides a cell culture device, comprising a shell, a roller, a partition plate, a filter box and a blower;

[0007] The bottom of the shell is rotatably connected to the roller; the bottom of the inner wall of the shell is connected to the partition plate; a tilted culture structure for tilting and shaking cells is provided inside the shell; a discharge structure for automatic discharge is provided on the side of the partition plate; a pushing structure for keeping the inside of the shell sealed is provided on the bottom of the inner wall of the shell; the side of the inner wall of the shell is connected to the blower; an air inlet window is provided on the side of the shell; the air outlet end of the blower is connected to the filter box; the filter box is connected to the inner wall of the shell; the side of the filter box passes through the side of the partition plate.

[0008] Preferably, the inclined culture structure includes a rotating motor, a turntable, a rotating connecting rod, a rotating bracket, a linkage rod, a supporting bracket and a culture dish; a motor bracket is provided on the bottom of the inner wall of the shell, and the motor bracket is connected to the rotating motor; the output end of the rotating motor is transmission-connected to the turntable; the bottom of the turntable is rotationally connected to the bottom of the inner wall of the shell; the side of the turntable is rotationally connected to a rotating connecting rod; the rotating connecting rod is rotationally connected to the rotating bracket; the side of the rotating bracket is rotationally connected to the linkage rod; the side of the rotating bracket is connected to the supporting bracket; the side of the supporting bracket is rotationally connected to the side of the inner wall of the shell; the top of the supporting bracket is connected to multiple groups of culture dishes; the multiple groups of supporting brackets and the culture dishes move synchronously through the linkage rod.

[0009] Preferably, the discharging structure includes a transverse slide rail, a vertical slide rail, a connecting rod, a second rotating connecting rod, a suction cup and a vacuum pump; the side of the transverse slide rail is connected to the partition plate; the side of the transverse slide rail is slidably connected to the vertical slide rail; the vertical slide rail is slidably connected to the connecting rod; the connecting rod is rotatably connected to the second rotating connecting rod; the end of the second rotating connecting rod away from the connecting rod is connected to the suction cup; the top of the suction cup is connected to the vacuum pump; and the vacuum pump is connected to the second rotating connecting rod.

[0010] Preferably, the pushing structure includes a telescopic rod, a blocking member, a pushing plate, a rotating door, a spring and a spring bracket; the side of the telescopic rod is connected to the bottom of the inner wall of the shell; the output end of the telescopic rod is transmission-connected to the pushing plate; the bottom of the inner wall of the shell is connected to multiple groups of blocking members; the multiple groups of blocking members are slidingly connected to the pushing plate; a through hole is provided on the bottom of the partition plate, and the side of the partition plate is rotationally connected to the rotating door; the bottom of the inner wall of the shell is connected to the spring bracket; the spring bracket is connected to the spring; and the spring is connected to the rotating door.

[0011] Preferably, the bottom of the suction cup is in an inverted bowl shape, and the inner wall of the suction cup is in contact and squeezed with the side of the culture dish; a sealing ring is provided on the side of the inner wall of the suction cup.

[0012] Preferably, a groove is provided on the top of the support bracket, a limiting piece is provided inside the groove, and the limiting piece is connected to the bottom side of the culture dish.

[0013] Preferably, a rotating shaft is provided on the side of the partition plate, the rotating shaft is rotatably connected to the rotating door, and a sealing member is provided on the side of the rotating door.

[0014] Preferably, the transverse slide rail and the vertical slide rail are power slide rails, and sliding stoppers are provided on both ends of the transverse slide rail and the vertical slide rail.

[0015] The above technical solution of the utility model has the following beneficial technical effects:

[0016] The utility model can effectively improve the efficiency and accuracy of cell culture through the device, and can effectively improve the loose structure of cells by tilting and shaking, thereby reducing the problem of cell clustering and aggregation, and the discrete cells can be easy to count, thereby reducing the counting difficulty of the staff. At the same time, the device can reduce the damage to the internal environment of the shell during the sampling of the culture dish by the combination of the suction cup and the rotating door, thereby reducing the external environment air from entering the shell of the device during the automatic sampling of the device, ensuring the purity of the culture dish, and the structure of the filter box and the blower of the device can ensure the internal ventilation environment of the device while filtering out dust and bacteria in the air. The combination of the spring and the rotating door can effectively improve the sealing stability of the device, and after the culture dish is discharged, the rotating door can be quickly closed to ensure the stability of the environment inside the shell. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings are only used to illustrate the principles, implementation methods, applications, characteristics and effects of the specific embodiments of the present application and other related contents, and shall not be considered as limitations of the present application.

[0018] In the drawings of the specification:

[0019] Figure 1 It is a front view of an embodiment of a cell culture device of the utility model;

[0020] Figure 2 This is a schematic diagram of the structure of the support bracket in the embodiment of the utility model;

[0021] Figure 3 This is a schematic diagram of the structure of the linkage rod in the embodiment of the utility model;

[0022] Figure 4 This is a schematic diagram of the structure of the spring in the embodiment of the utility model;

[0023] Figure 5 It is a schematic diagram of the structure of the culture dish in the embodiment of the utility model.

[0024] The reference numerals in the above drawings are described as follows:

[0025] 1. Shell; 2. Roller; 3. Partition plate; 4. Rotating motor; 5. Turntable; 6. Rotating connecting rod 1; 7. Rotating bracket; 8. Linking rod; 9. Support bracket; 10. Culture dish; 11. Horizontal slide rail; 12. Vertical slide rail; 13. Connecting rod; 14. Rotating connecting rod 2; 15. Suction cup; 16. Vacuum pump; 17. Telescopic rod; 18. Blocking member; 19. Push plate; 20. Rotating door; 21. Spring; 22. Spring bracket; 23. Filter box; 24. Blower. DETAILED DESCRIPTION

[0026] In order to explain in detail the possible application scenarios, technical principles, specific schemes that can be implemented, and the purposes and effects that can be achieved, the following is a detailed description of the specific embodiments listed in conjunction with the accompanying drawings. The embodiments described herein are only used to more clearly illustrate the technical solutions of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0027] Reference to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or association with other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the various technical features mentioned in the embodiments can be combined in any way to form a corresponding implementable technical solution.

[0028] Unless otherwise defined, the technical terms used in this document have the same meanings as those generally understood by those skilled in the art to which this application belongs; the use of relevant terms in this document is only for describing specific embodiments and is not intended to limit this application.

[0029] In the description of this application, the term "and / or" is an expression used to describe the logical relationship between objects, indicating that three relationships may exist, for example, X and / or Y, which means: X exists, Y exists, and X and Y exist at the same time. In addition, the character " / " in this article generally indicates that the objects before and after are in a logical relationship of "or".

[0030] In the present application, terms such as “first” and “second” are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship of quantity, priority or sequence between these entities or operations.

[0031] Without further limitations, in this application, the words "include", "comprises", "has" or other similar expressions used in the sentences are intended to cover non-exclusive inclusion. These expressions do not exclude the presence of additional elements in the process, method or product including the elements, so that the process, method or product including a series of elements may include not only those limited elements, but also other elements not explicitly listed, or also include elements inherent to such process, method or product.

[0032] Similar to the understanding in the Examination Guidelines, in this application, expressions such as "greater than", "less than", "exceed" and the like are understood to exclude the number itself; expressions such as "above", "below", "within" and the like are understood to include the number itself. In addition, in the description of the embodiments of this application, "multiple" means more than two (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups", "multiple times", etc., unless otherwise clearly and specifically limited.

[0033] In the description of the embodiments of the present application, space-related expressions used, such as "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "vertical", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicate the orientation or position relationship based on the orientation or position relationship shown in the specific embodiments or drawings, and are only for the convenience of describing the specific embodiments of the present application or facilitating the reader's understanding, and do not indicate or imply that the referred device or component must have a specific position, a specific orientation, or be constructed or operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.

[0034] Unless otherwise expressly specified or limited, in the description of the embodiments of the present application, the terms such as "install", "connect", "connect", "fix", "set", etc. used should be understood in a broad sense. For example, the "connection" can be a fixed connection, a detachable connection, or an integrated setting; it can be a mechanical connection, an electrical connection, or a communication connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. For technicians in the technical field to which the present application belongs, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0035] Embodiment 1

[0036] like Figure 1-Figure 5 As shown, a cell culture device proposed by the utility model includes a housing 1, a roller 2, a partition plate 3, a filter box 23 and a blower 24;

[0037] The bottom of the shell 1 is rotatably connected to the roller 2; the bottom of the inner wall of the shell 1 is connected to the partition plate 3; an inclined culture structure for tilting and shaking cells is provided inside the shell 1; a discharge structure for automatic discharge is provided on the side of the partition plate 3; a pushing structure for keeping the inside of the shell 1 sealed is provided on the bottom of the inner wall of the shell 1; the side of the inner wall of the shell 1 is connected to the blower 24; an air inlet window is provided on the side of the shell 1; the air outlet end of the blower 24 is connected to the filter box 23; the filter box 23 is connected to the inner wall of the shell 1; the side of the filter box 23 passes through the side of the partition plate 3.

[0038] Furthermore, the inclined culture structure includes a rotating motor 4, a rotating disk 5, a rotating connecting rod 6, a rotating bracket 7, a linkage rod 8, a supporting bracket 9 and a culture dish 10; a motor bracket is provided on the bottom of the inner wall of the shell 1, and the motor bracket is connected to the rotating motor 4; the output end of the rotating motor 4 is transmission-connected to the rotating disk 5; the bottom of the rotating disk 5 is rotationally connected to the bottom of the inner wall of the shell 1; the side of the rotating disk 5 is rotationally connected to the rotating connecting rod 6; the rotating connecting rod 6 is rotationally connected to the rotating bracket 7; the side of the rotating bracket 7 is rotationally connected to the linkage rod 8; the side of the rotating bracket 7 is connected to the supporting bracket 9; the side of the supporting bracket 9 is rotationally connected to the side of the inner wall of the shell 1; the top of the supporting bracket 9 is connected to multiple groups of culture dishes 10; multiple groups of supporting brackets 9 and the culture dishes 10 move synchronously through the linkage rod 8;

[0039] Furthermore, the discharging structure includes a transverse slide rail 11, a vertical slide rail 12, a connecting rod 13, a rotating connecting rod 14, a suction cup 15 and a vacuum pump 16; the side of the transverse slide rail 11 is connected to the partition plate 3; the side of the transverse slide rail 11 is slidably connected to the vertical slide rail 12; the vertical slide rail 12 is slidably connected to the connecting rod 13; the connecting rod 13 is rotatably connected to the rotating connecting rod 14; the end of the rotating connecting rod 14 away from the connecting rod 13 is connected to the suction cup 15; the top of the suction cup 15 is connected to the vacuum pump 16; the vacuum pump 16 is connected to the rotating connecting rod 14;

[0040] Furthermore, the pushing structure includes a telescopic rod 17, a blocking member 18, a pushing plate 19, a rotating door 20, a spring 21 and a spring bracket 22; the side of the telescopic rod 17 is connected to the bottom of the inner wall of the shell 1; the output end of the telescopic rod 17 is transmission-connected to the pushing plate 19; the bottom of the inner wall of the shell 1 is connected to multiple groups of blocking members 18; the multiple groups of blocking members 18 are slidingly connected to the pushing plate 19; a through hole is provided on the bottom of the partition plate 3, and the side of the partition plate 3 is rotationally connected to the rotating door 20; the bottom of the inner wall of the shell 1 is connected to the spring bracket 22; the spring bracket 22 is connected to the spring 21; the spring 21 is connected to the rotating door 20.

[0041] In this embodiment, the device can realize automatic sampling and discharging while improving the discrete effect of the cell culture process, thereby reducing the technical difficulty while ensuring the stability of the device. The structure of the turntable 5 and the rotating connecting rod 6 can realize the left and right tilting effect of the device, reducing the risk of cell clustering, making the cells more discrete and easy to count. At the same time, the structure of the suction cup 15 and the vacuum pump 16 can effectively improve the adsorption stability of the device. At the same time, the adsorption and transportation method can effectively improve the pollution isolation effect of the device, avoiding the introduction of pollution during the discharging process while improving the automation efficiency of the device. The culture dish 10 is transported between the blocking members 18 by the suction cup 15, thereby achieving the effects of pushing the discharging and automatically closing the door of the device.

[0042] Embodiment 2

[0043] like Figure 1-Figure 5 As shown, a cell culture device proposed by the utility model, compared with the first embodiment, in this embodiment, the bottom of the suction cup 15 is in an inverted bowl shape, and the inner wall of the suction cup 15 is in contact and squeezed with the side of the culture dish 10; a sealing ring is provided on the side of the inner wall of the suction cup 15; the culture dish 10 can be adsorbed and transported through the structure of the suction cup 15, and the structure of the sealing ring can effectively improve the adsorption stability of the device when the vacuum pump 16 generates negative pressure.

[0044] Those skilled in the art will appreciate that, although some embodiments herein include certain features included in other embodiments but not other features, the combination of features of different embodiments is meant to be within the scope of the present application and form different embodiments. For example, in the claims, any one of the claimed embodiments may be used in any combination.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them; although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application, and they should all be included in the scope of the claims and specification of the present application. In particular, as long as there is no structural conflict, the various technical features mentioned in the various embodiments can be combined in any way. The present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions that fall within the scope of the claims.

Claims

1. A cell culture device, characterized in that: It comprises a housing (1), a roller (2), a partition plate (3), a filter box (23) and a blower (24); The bottom of the shell (1) is rotatably connected to the roller (2); the bottom of the inner wall of the shell (1) is connected to the partition plate (3); an inclined culture structure for tilting and shaking cells is provided inside the shell (1); a discharge structure for automatic discharge is provided on the side of the partition plate (3); a pushing structure for keeping the inside of the shell (1) sealed is provided on the bottom of the inner wall of the shell (1); the side of the inner wall of the shell (1) is connected to the blower (24); an air inlet window is provided on the side of the shell (1); the air outlet end of the blower (24) is connected to the filter box (23); the filter box (23) is connected to the inner wall of the shell (1); and the side of the filter box (23) passes through the side of the partition plate (3).

2. A cell culture device according to claim 1, characterized in that: The inclined culture structure comprises a rotating motor (4), a rotating disk (5), a rotating connecting rod (6), a rotating bracket (7), a linkage rod (8), a supporting bracket (9) and a culture dish (10); a motor bracket is provided on the bottom of the inner wall of the shell (1), and the motor bracket is connected to the rotating motor (4); the output end of the rotating motor (4) is transmission-connected to the rotating disk (5); the bottom of the rotating disk (5) is rotationally connected to the bottom of the inner wall of the shell (1); the side of the rotating disk (5) is rotationally connected to the rotating connecting rod (6); the rotating connecting rod (6) is rotationally connected to the rotating bracket (7); the side of the rotating bracket (7) is rotationally connected to the linkage rod (8); the side of the rotating bracket (7) is connected to the supporting bracket (9); the side of the supporting bracket (9) is rotationally connected to the side of the inner wall of the shell (1); the top of the supporting bracket (9) is connected to multiple groups of culture dishes (10); the multiple groups of supporting brackets (9) and the culture dishes (10) move synchronously through the linkage rod (8).

3. A cell culture device according to claim 1, characterized in that: The discharging structure comprises a transverse slide rail (11), a vertical slide rail (12), a connecting rod (13), a second rotating connecting rod (14), a suction cup (15) and a vacuum pump (16); the side of the transverse slide rail (11) is connected to the partition plate (3); the side of the transverse slide rail (11) is slidably connected to the vertical slide rail (12); the vertical slide rail (12) is slidably connected to the connecting rod (13); the connecting rod (13) is rotatably connected to the second rotating connecting rod (14); the end of the second rotating connecting rod (14) away from the connecting rod (13) is connected to the suction cup (15); the top of the suction cup (15) is connected to the vacuum pump (16); and the vacuum pump (16) is connected to the second rotating connecting rod (14).

4. A cell culture device according to claim 1, characterized in that: The pushing structure comprises a telescopic rod (17), a blocking member (18), a pushing plate (19), a rotating door (20), a spring (21) and a spring bracket (22); the side of the telescopic rod (17) is connected to the bottom of the inner wall of the shell (1); the output end of the telescopic rod (17) is transmission-connected to the pushing plate (19); the bottom of the inner wall of the shell (1) is connected to multiple groups of blocking members (18); the multiple groups of blocking members (18) are slidably connected to the pushing plate (19); a through hole is provided on the bottom of the partition plate (3); the side of the partition plate (3) is rotationally connected to the rotating door (20); the bottom of the inner wall of the shell (1) is connected to the spring bracket (22); the spring bracket (22) is connected to the spring (21); and the spring (21) is connected to the rotating door (20).

5. A cell culture device according to claim 1, characterized in that: The bottom of the suction cup (15) is in the shape of an inverted bowl, and the inner wall of the suction cup (15) is in contact and squeezed with the side of the culture dish (10); a sealing ring is provided on the side of the inner wall of the suction cup (15).

6. A cell culture device according to claim 1, characterized in that: A groove is provided on the top of the support bracket (9), a limiting piece is provided inside the groove, and the limiting piece is connected to the bottom side of the culture dish (10).

7. A cell culture device according to claim 1, characterized in that: A rotating shaft is provided on the side of the partition plate (3), the rotating shaft is rotatably connected to the rotating door (20), and a sealing member is provided on the side of the rotating door (20).

8. The cell culture device according to claim 1, characterized in that: The transverse slide rail (11) and the vertical slide rail (12) are power slide rails, and sliding blocking members are arranged at both ends of the transverse slide rail (11) and the vertical slide rail (12).

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