Automatic split charging and filling device for solid culture medium

Abstract

The utility model provides an automatic split charging and filling device for a solid culture medium, and relates to the technical field of experimental devices, the automatic split charging and filling device for the solid culture medium comprises a box body; the tray is telescopically arranged at the opening of the box body, and a sealing plate capable of sealing the opening is arranged on the tray; the turnover frame is rotationally arranged on the tray; the culture dish is detachably arranged on the overturning frame; the turnover driving mechanism is arranged on the tray and is connected with the turnover frame; the filling mechanism is used for filling a culture medium into the culture dish. The turnover frame is driven by the turnover driving mechanism to turn over, the culture dish is inverted, and condensate water in the culture dish is discharged. Therefore, an experimenter does not need to manually pour out condensate water in the culture dishes one by one, the workload of the experimenter is reduced, and the working efficiency is improved.

Description

Technical Field

[0001] The present application relates to the technical field of experimental devices, and in particular to an automatic dispensing and filling device for solid culture medium. Background Art

[0002] Solid culture medium refers to a culture medium that is solid at normal culture temperature. Its processing method is to add 1.5% to 2.0% coagulant (such as agar) to the liquid culture medium, heat it to 100°C to dissolve it, and then cool and solidify it at a temperature below 40°C to make it a solid state, namely solid culture medium. Solid culture medium is widely used in the isolation, identification, preservation, counting and observation of colony characteristics of microorganisms.

[0003] A microbial solid culture medium quantitative filler is a device used to quantitatively add solid culture medium to a culture dish. For example, the utility model with publication number CN211497589U discloses a microbial solid culture medium quantitative filler. The microbial solid culture medium quantitative filler is provided with an electric heating wire. After the culture medium solution is introduced into the inner cavity of the storage tank, the electric heating wire is energized by a battery to heat the culture medium solution in the inner cavity to more than 40 degrees to prevent solidification. At the same time, a hydraulic pump is provided. When an experiment is required, the hydraulic pump is started to introduce a certain volume of culture medium solution into the inner cavity of the metering tank, and then introduced into the culture dish to prepare a solid flat plate.

[0004] However, after the culture medium is quantitatively added to the culture dish through the microbial solid culture medium quantitative filler, the culture medium needs to be cooled naturally. In order to prevent the culture medium from being contaminated during the cooling process, the culture dish cover is closed on the culture dish to seal it. After the cooling is completed, a certain amount of condensed water will be generated on the inner wall of the culture dish and the culture dish cover. After the condensed water flows to the surface of the culture medium, it will cause the surface of the culture medium to be too wet, and the inoculated bacteria will easily spread, affecting the experimenter's observation and statistics of the strains. Therefore, the experimenter needs to pour out the condensed water in the culture dish one by one after the cooling is completed, which increases the workload of the experimenter and reduces the work efficiency. Utility Model Content

[0005] The purpose of the present application is to provide an automatic solid culture medium dispensing and filling device, which is used to solve the problem that after the microbial solid culture medium quantitative filler in the related art is filled and cooled, the experimenter needs to pour out the condensed water in the culture dish one by one, which leads to a large workload and low work efficiency.

[0006] The present application provides a solid culture medium automatic filling and filling device adopts the following technical solution:

[0007] A solid culture medium automatic dispensing and filling device, comprising:

[0008] A box body, wherein the box body is provided with an opening;

[0009] A tray, the tray being retractably disposed at the opening of the box body, and the tray being provided with a sealing plate capable of sealing the opening;

[0010] A turning frame, the turning frame is rotatably arranged on the tray;

[0011] A culture dish, which is detachably mounted on the turning frame;

[0012] A turning drive mechanism, which is disposed on the tray and connected to the turning frame, and is used to drive the turning frame to rotate;

[0013] A filling mechanism is used to inject culture medium into the culture dish.

[0014] Optionally, a limiting mechanism is further included, wherein the flip frame is provided with a positioning groove for accommodating the culture dish, the culture dish is provided with a limiting portion, the limiting mechanism includes a limiting member and a driving assembly, the driving assembly is provided on the flip frame and connected to the limiting member, and the driving assembly is used to drive the limiting member to act on the limiting portion to limit the culture dish from escaping from the positioning groove.

[0015] Optionally, the limiting member is a strip plate, and two strip plates are provided. The two strip plates are slidably arranged on the flip frame, and the driving assembly is used to drive the two strip plates to move towards or away from each other, and the limiting portion is an annular groove provided on the outer peripheral wall of the culture dish, and the strip plate can be clamped in the annular groove.

[0016] Optionally, the driving assembly includes a rotating shaft and a first worm gear, a rack is provided on the strip plate, the rotating shaft is rotatably arranged on the flip frame, a gear and a first worm wheel are provided on the rotating shaft, the racks on the two strip plates are respectively meshed with the gears, and the first worm gear is rotatably arranged on the flip frame and meshed with the first worm wheel.

[0017] Optionally, the flipping drive mechanism includes a second worm, a pivot is provided on the flipping frame, the flipping frame is rotatably arranged on the tray via the pivot, a second worm wheel is provided on the pivot, the second worm is rotatably arranged on the tray and meshes with the second worm wheel, and the second worm is penetrated through the sealing plate.

[0018] Optionally, a drainage groove is provided on the side wall of the box body, a through groove penetrating the drainage groove is provided on the inner wall of the box body, water retaining plates are provided on both sides of the tray, and the end of the tray opposite to the sealing plate can be inserted into the drainage groove through the through groove.

[0019] Optionally, it also includes an ultraviolet sterilization lamp, which is arranged in the box and above the turning frame.

[0020] Optionally, the filling mechanism includes a translation assembly, a storage tank, a heating element, a metering pump and a filling head, the storage tank is used to store culture medium, the heating element is arranged on the storage tank, the translation assembly is arranged on the box body, the filling head is located in the box body and connected to the translation assembly, the filling head is connected to the outlet end of the metering pump through a first electric heating tube, and the inlet end of the metering pump is connected to the storage tank through a second electric heating tube.

[0021] In summary, the present application includes at least one of the following beneficial technical effects: when it is necessary to add culture medium to the culture dish, first pull the tray out of the box, then place the culture dish on the flip rack, and then push the tray into the box. When the tray is completely pushed into the box, the sealing plate just blocks the opening of the box, and the box is closed, so that the culture dish is in a closed space to reduce external pollution. Then, the culture medium is quantitatively added to the culture dish through the filling mechanism. When the filling is completed, the culture dish filled with the culture medium is placed in the box for cooling. After the cooling is completed, the liquid culture medium solidifies into a solid culture medium and adheres to the inner wall of the culture dish. At the same time, a certain amount of condensed water will accumulate in the culture dish. At this time, the flip drive mechanism drives the flip rack to flip, the culture dish is inverted, and the condensed water in the culture dish is discharged. There is no need for the experimenter to manually pour out the condensed water in the culture dish one by one, thereby reducing the workload of the experimenter and improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the structure of the automatic filling and filling device for solid culture medium in the embodiment of the present application;

[0023] Figure 2 It is a cross-sectional view of the solid culture medium automatic dispensing and filling device from the first perspective in the embodiment of the present application;

[0024] Figure 3 It is a cross-sectional view of the second viewing angle of the automatic dispensing and filling device for solid culture medium in the embodiment of the present application;

[0025] Figure 4 It is a cross-sectional view of the solid culture medium automatic dispensing and filling device from a third perspective in the embodiment of the present application;

[0026] Figure 5 for Figure 2 A partial enlarged schematic diagram of part A;

[0027] Figure 6 for Figure 3 A partial enlarged schematic diagram of part B.

[0028] Description of reference numerals:

[0029] 10. Box body; 11. Opening; 12. Drainage groove; 13. Through groove; 14. Guide rod; 15. Slide rail; 16. Discharge outlet;

[0030] 20. tray; 21. cover plate; 211. handle; 22. water retaining plate; 24. sliding sleeve;

[0031] 30. Turning frame; 31. Guide rail; 32. Pivot; 321. Second worm gear; 33. Positioning groove; 34. Avoidance groove;

[0032] 40. culture dish; 41. annular groove; 50. turning drive mechanism; 51. second worm;

[0033] 60. Filling mechanism; 61. Storage tank; 63. Dosing pump; 64. Filling head; 65. First electric heating tube; 66. Second electric heating tube; 67. Translation seat; 671. Screw sleeve; 68. Screw rod; 69. Translation motor;

[0034] 70. Strip plate; 71. Sliding sleeve; 72. Rack;

[0035] 80. driving assembly; 81. rotating shaft; 811. gear; 812. first worm gear; 82. first worm;

[0036] 90. Ultraviolet germicidal lamp. DETAILED DESCRIPTION

[0037] The following is combined with Figure 1 -Attached Figure 6 , further details of this application are given.

[0038] The embodiment of the present application discloses an automatic filling and dispensing device for solid culture medium.

[0039] Reference Figures 1 to 3 A solid culture medium automatic filling and dispensing device includes a box body 10, a tray 20, a turning frame 30, a culture dish 40, a turning drive mechanism 50, a filling mechanism 60, a limiting mechanism and an ultraviolet sterilization lamp 90. The box body 10 is provided with an opening 11, and the tray 20 is telescopically arranged at the opening 11 of the box body 10. More specifically, the box body 10 is provided with a slide rail 15, and the tray 20 is provided with a sliding sleeve 24. The tray 20 is slidably sleeved on the slide rail 15 through the sliding sleeve 24.

[0040] The tray 20 is provided with a sealing plate 21 capable of sealing the opening 11 , and the sealing plate 21 is provided with a handle 211 . When the tray 20 is completely pushed into the box body 10 , the sealing plate 21 just seals the opening 11 of the box body 10 , thereby closing the box body 10 .

[0041] A drainage groove 12 is provided on the side wall of the box body 10, a discharge port 16 communicating with the drainage groove 12 and the outside of the box body 10 is provided on the outer wall of the box body 10, a through groove 13 penetrating the drainage groove 12 is provided on the inner wall of the box body 10, water retaining plates 22 are provided on both sides of the tray 20, and the end of the tray 20 opposite to the sealing plate 21 can be inserted into the drainage groove 12 through the through groove 13.

[0042] The turning frame 30 is rotatably disposed on the tray 20 . The ultraviolet sterilization lamp 90 is disposed in the box 10 . The ultraviolet sterilization lamp 90 is located above the turning frame 30 .

[0043] Reference Figures 2 to 4 The flip driving mechanism 50 is disposed on the tray 20 and connected to the flip frame 30. The flip driving mechanism 50 is used to drive the flip frame 30 to rotate. In an optional embodiment, the specific structure of the flip driving mechanism 50 and the specific connection relationship with the flip frame 30 are as follows:

[0044] The flipping drive mechanism 50 includes a second worm 51, a pivot 32 is provided on the flipping frame 30, the flipping frame 30 is rotatably arranged on the tray 20 via the pivot 32, a second worm gear 321 is provided on the pivot 32, the second worm 51 is rotatably arranged on the tray 20 and meshes with the second worm gear 321, and the second worm 51 is passed through the sealing plate 21. The second worm 51 can be rotated to drive the second worm gear 321 and the pivot 32 to rotate, thereby driving the flipping frame 30 to flip.

[0045] Reference Figure 2 , Figure 3 , Figure 5 and Figure 6 The culture dish 40 is detachably arranged on the turning frame 30. More specifically, a positioning groove 33 for accommodating the culture dish 40 is provided on the turning frame 30. A limiting portion is provided on the culture dish 40. The limiting mechanism includes a limiting member and a driving assembly 80. The driving assembly 80 is arranged on the turning frame 30 and connected to the limiting member. The driving assembly 80 is used to drive the limiting member to act on the limiting portion to limit the culture dish 40 from escaping from the positioning groove 33.

[0046] In an optional embodiment, the limiting member is a strip plate 70, and two strip plates 70 are provided. The two strip plates 70 are slidably arranged on the turning frame 30. More specifically, a guide rail 31 is provided on the turning frame 30, and a sliding sleeve 71 is provided on the two strip plates 70. The sliding sleeve 71 is slidably arranged on the guide rail 31.

[0047] The driving assembly 80 is used to drive the two strip plates 70 to move towards or away from each other. The limiting portion is an annular groove 41 arranged on the outer peripheral wall of the culture dish 40. The strip plate 70 can be snapped into the annular groove 41. The turning frame 30 is provided with an avoidance groove 34 that penetrates the positioning groove 33 and is used to avoid the strip plate 70.

[0048] In an optional embodiment, the specific structure of the driving assembly 80 and the specific connection relationship between the driving assembly 80 and the two strip plates 70 are as follows: the driving assembly 80 includes a rotating shaft 81 and a first worm 82, a rack 72 is provided on the strip plate 70, the rotating shaft 81 is rotatably arranged on the flip frame 30, two rotating shafts 81 are provided, gears 811 are respectively provided on the two rotating shafts 81, and a first worm gear 812 is also provided on one of the rotating shafts 81, the racks 72 on the two strip plates 70 are respectively meshed with the gears 811, and the first worm gear 82 is rotatably arranged on the flip frame 30 and meshed with the first worm gear 812.

[0049] When the culture dish 40 is placed in the positioning groove 33, the first worm gear 812, the rotating shaft 81 and the gear 811 can be driven to rotate by rotating the first worm 82, and then the two strip plates 70 are driven to move towards each other under the interaction of the gear 811 and the rack 72, so that the strip plates 70 pass through the avoidance groove 34 and engage with the annular groove 41, thereby limiting the culture dish 40 and preventing the culture dish 40 from falling off the turning frame 30.

[0050] Reference Figures 1 to 3 The filling mechanism 60 is used to inject culture medium into the culture dish 40. In an optional embodiment, the specific structure of the filling mechanism 60 is as follows:

[0051] The filling mechanism 60 includes a translation assembly, a storage tank 61, a heating element, a metering pump 63 and a filling head 64. The storage tank 61 is used to store culture medium. The heating element is arranged on the storage tank 61. The heating element is used to heat the culture medium in the storage tank 61. The heating element can adopt an electric heating wire, which can be wrapped around the outer peripheral side of the storage tank 61. The translation assembly is arranged on the box body 10. More specifically, the translation assembly includes a translation seat 67, a screw rod 68 and a translation motor 69. A guide rod 14 is provided in the box body 10. The translation seat 67 is slidably provided on the guide rod 14. A screw sleeve 671 is provided on the translation seat 67. The screw rod 68 is rotatably provided in the box body 10 and is screwed to the screw sleeve 671. The translation motor 69 is fixed on the box body 10 and connected to the screw rod 68.

[0052] The filling head 64 is located inside the box body 10 and is fixedly connected to the translation seat 67 of the translation assembly. The filling head 64 is connected to the outlet end of the metering pump 63 through the first electric heating pipe 65. The first electric heating pipe 65 is a hose. The inlet end of the metering pump 63 is connected to the storage tank 61 through the second electric heating pipe 66.

[0053] The implementation principle of the automatic solid culture medium dispensing and filling device of the present embodiment is as follows: when the culture medium needs to be filled into the culture dish 40, the tray 20 is first pulled out of the box 10, and then the culture dish 40 is placed in the positioning groove 33 of the flip frame 30, and then the first worm 82 is rotated, and the first worm gear 812, the rotating shaft 81 and the gear 811 are driven to rotate by the first worm 82, and then the two strip plates 70 are driven to move towards each other under the interaction between the gear 811 and the rack 72, so that the strip plates 70 pass through the avoidance groove 34 and engage with the annular groove 41, so as to limit the culture dish 40.

[0054] Then, the tray 20 is pushed into the box 10. When the tray 20 is completely pushed into the box 10, the sealing plate 21 is just sealed at the opening 11 of the box 10, and the box 10 is sealed, so that the culture dish 40 is in a closed space to reduce external contamination. At the same time, when the tray 20 is completely pushed into the box 10, the end of the tray 20 opposite to the sealing plate 21 can be inserted into the drainage groove 12 through the through groove 13.

[0055] Next, the translation motor 69 drives the screw rod 68 to rotate, and the screw rod 68 drives the translation seat 67 and the filling head 64 to move, and the filling head 64 is moved above the culture dish 40. Then, the quantitative pump 63 is started to quantitatively fill the culture medium in the storage tank 61 into the culture dish 40. During the filling process, the culture medium is heated by the heating element, the first electric heating tube 65 and the second electric heating tube 66 to maintain the fluidity of the culture medium.

[0056] After the filling is completed, the culture dish 40 filled with the culture medium is placed in the box 10 for cooling. When the cooling is completed, the liquid culture medium solidifies into a solid culture medium and adheres to the inner wall of the culture dish 40, and a certain amount of condensed water accumulates in the culture dish 40. At this time, the second worm 51 is rotated, and the second worm gear 321 and the pivot 32 are driven to rotate by the second worm 51, and the flip frame 30 is driven to flip, and the culture dish 40 is turned upside down, so that the condensed water in the culture dish 40 flows to the tray 20, and the condensed water on the tray 20 flows into the drain groove 12 from the end of the tray 20 opposite to the sealing plate 21, and is discharged to the outside of the box 10 from the discharge port 16.

[0057] Next, the flipping frame 30 and the culture dish 40 are flipped and straightened by the flipping drive mechanism 50, and then the tray 20 is pulled out of the box body 10, and then the driving component 80 drives the strip plate 70 to separate from the annular groove 41, and the culture dish 40 containing the solid culture medium is taken out to complete the quantitative filling of the solid culture medium.

[0058] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. The same components are represented by the same figure marks. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A solid culture medium automatic filling and dispensing device, characterized in that: include: A box body (10), wherein the box body (10) is provided with an opening (11); A tray (20), the tray (20) being retractably disposed at the opening (11) of the box body (10), and the tray (20) being provided with a sealing plate (21) capable of sealing the opening (11); A turning frame (30), the turning frame (30) being rotatably disposed on the tray (20); a culture dish (40), wherein the culture dish (40) is detachably mounted on the turning frame (30); a turning drive mechanism (50), the turning drive mechanism (50) being arranged on the tray (20) and connected to the turning frame (30), the turning drive mechanism (50) being used to drive the turning frame (30) to rotate; A filling mechanism (60), wherein the filling mechanism (60) is used to inject culture medium into the culture dish (40).

2. The solid culture medium automatic filling and dispensing device according to claim 1, characterized in that: The invention also comprises a limiting mechanism, wherein the turning frame (30) is provided with a positioning groove (33) for accommodating the culture dish (40), and the culture dish (40) is provided with a limiting portion, and the limiting mechanism comprises a limiting member and a driving assembly (80), wherein the driving assembly (80) is provided on the turning frame (30) and connected to the limiting member, and the driving assembly (80) is used to drive the limiting member to act on the limiting portion to limit the culture dish (40) from escaping from the positioning groove (33).

3. The automatic filling and dispensing device for solid culture medium according to claim 2, characterized in that: The limiting member is a strip plate (70), and two strip plates (70) are provided. The two strip plates (70) are slidably arranged on the turning frame (30). The driving assembly (80) is used to drive the two strip plates (70) to move towards or away from each other. The limiting portion is an annular groove (41) arranged on the outer peripheral wall of the culture dish (40), and the strip plate (70) can be clamped with the annular groove (41).

4. The automatic filling and dispensing device for solid culture medium according to claim 3, characterized in that: The driving assembly (80) comprises a rotating shaft (81) and a first worm (82); a rack (72) is provided on the strip plate (70); the rotating shaft (81) is rotatably arranged on the flip frame (30); a gear (811) and a first worm wheel (812) are provided on the rotating shaft (81); the racks (72) on the two strip plates (70) are respectively meshed with the gear (811); the first worm (82) is rotatably arranged on the flip frame (30) and meshed with the first worm wheel (812).

5. The automatic filling and dispensing device for solid culture medium according to claim 1, characterized in that: The flipping driving mechanism (50) comprises a second worm (51); a pivot (32) is provided on the flipping frame (30); the flipping frame (30) is rotatably mounted on the tray (20) via the pivot (32); a second worm wheel (321) is provided on the pivot (32); the second worm (51) is rotatably mounted on the tray (20) and meshes with the second worm wheel (321); and the second worm (51) is passed through the sealing plate (21).

6. The automatic filling and dispensing device for solid culture medium according to claim 1, characterized in that: A drainage groove (12) is provided on the side wall of the box body (10), a through groove (13) penetrating the drainage groove (12) is provided on the inner side wall of the box body (10), water retaining plates (22) are provided on both sides of the tray (20), and an end of the tray (20) opposite to the sealing plate (21) can be inserted into the drainage groove (12) through the through groove (13).

7. The automatic filling and dispensing device for solid culture medium according to claim 1, characterized in that: It also includes an ultraviolet sterilization lamp (90), which is arranged in the box (10) and located above the turning frame (30).

8. The solid culture medium automatic filling and dispensing device according to claim 1, characterized in that: The filling mechanism (60) comprises a translation assembly, a storage tank (61), a heating element, a metering pump (63) and a filling head (64); the storage tank (61) is used to store culture medium; the heating element is arranged on the storage tank (61); the translation assembly is arranged on the box body (10); the filling head (64) is located in the box body (10) and is connected to the translation assembly; the filling head (64) is connected to the outlet end of the metering pump (63) through a first electric heating tube (65); and the inlet end of the metering pump (63) is connected to the storage tank (61) through a second electric heating tube (66).

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