Cell culture device capable of adjusting gas concentration

By introducing internal and external pressure difference display device and automatic gas regulating device into the cell culture device, the problem of lowering gas concentration during cell culture is solved, automatic regulation and stability of gas concentration is achieved, and the operation process is simplified.

CN222331934UActive Publication Date: 2025-01-10ANHUI CHUANGJIA SAFETY ENVIRONMENT TECH CO LTD
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Patent Information

Application Number
CN202420947656.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-30
Publication Date
2025-01-10
Estimated Expiration
2034-04-30

AI Technical Summary

Technical Problem

During the cell culture process, the existing cell culture device gradually decreases due to the respiration of cells during the cell culture process, which requires frequent adjustment and is troublesome to operate.

Method used

A cell culture device including an incubator, an air regulating device and a telescopic device is designed to automatically adjust the gas concentration through an internal and external pressure difference display device and an automatic gas regulating device. The test tube is fixed by a rotating motor and a telescopic device, and the internal and external pressure is passed. The difference display device and the air regulating device automatically replenishes air to ensure the stability of the gas concentration.

Benefits of technology

It realizes automatic adjustment of gas concentration during cell culture, reduces the frequency of manual operation, and improves the stability and efficiency of gas inside the incubator.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the field of cell culture, and particularly discloses a cell culture device capable of adjusting gas concentration, which comprises an incubator, a gas adjusting device and a telescopic device, an internal and external pressure difference display device is arranged on the side surface of the incubator, an air adjusting device is arranged at the top of the incubator, a rotating motor is arranged at the bottom of the incubator, a motor shaft is arranged on the rotating motor, a rotating plate is arranged on the motor shaft, the rotating plate is arranged at the inner bottom of the incubator, and a telescopic device is arranged on the rotating plate. A fixing frame is arranged at the top of the telescopic device, test tubes are arranged on the fixing frame, a tube taking opening is formed in the top of the incubator, a cover body is arranged on the tube taking opening, a spring shaft is arranged between the cover body and the tube taking opening, and foot supports are arranged at the bottom of the incubator; according to the cell culture device, the gas concentration can be adjusted, the cell culture device is convenient to take out, and the internal pressure of the culture box is convenient.
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Description

Technical Field

[0001] The utility model relates to the field of cell culture, in particular to a cell culture device capable of adjusting gas concentration. Background Art

[0002] Cell biology is a discipline that studies life processes at the cellular level. In the early days, cytology was mainly based on morphological description. Later, cytology absorbed the achievements of molecular biology and went deep into the ultrastructural level. It mainly studied biological processes such as cell growth, metabolism and heredity. Cytology also developed into cell biology, and the culture of cells requires cell culture equipment.

[0003] In the process of cell culture in the existing cell culture device, the gas concentration inside the culture device becomes lower and lower due to the respiration of cells, and needs to be adjusted continuously, which is very troublesome. Therefore, a cell culture device with adjustable gas concentration is proposed. Utility Model Content

[0004] The purpose of the utility model is to provide a cell culture device capable of adjusting gas concentration, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture device with adjustable gas concentration, comprising an incubator, a gas regulating device, and a telescopic device; an internal and external pressure difference display device is arranged on the side of the incubator, a gas regulating device is arranged on the top of the incubator, a rotating motor is arranged on the bottom of the incubator, a motor shaft is arranged on the rotating motor, a rotating plate is arranged on the motor shaft, the rotating plate is arranged at the bottom of the incubator, a telescopic device is arranged on the rotating plate, a fixing frame is arranged on the top of the telescopic device, a test tube is arranged on the fixing frame, a tube taking port is arranged on the top of the incubator, a cover body is arranged on the tube taking port, a spring shaft is arranged between the cover body and the tube taking port, and a foot support is arranged on the bottom of the incubator.

[0006] Preferably, a connecting tube is provided inside the internal and external pressure difference display device, the connecting tube has a U-shaped structure, a filter screen port is provided on one end of the connecting tube, a connecting port is provided on the other end of the connecting tube, the connecting port is connected to the incubator, and liquid is provided inside the connecting tube.

[0007] Preferably, the air regulating device is provided with an air intake pipe, a filter port is provided on the top of the air intake pipe, a sleeve is provided inside the air regulating device and is sleeved on the outside of the air intake pipe, a spring support plate is provided on the bottom of the sleeve, a slot is provided between the spring support plate and the sleeve, a plurality of spring bodies are provided on the spring support plate, a blocking plate is provided on the top of the spring body, and the blocking plate is provided at the bottom of the air intake pipe.

[0008] Preferably, a telescopic rod is provided on the telescopic device, a supporting plate is provided on the telescopic rod, and a fixing frame is provided on the top of the supporting plate.

[0009] Preferably, the fixing frame is provided with a top plate, the top plate is provided with a support rod, a hole groove is provided in the middle of the top plate, and the test tube is arranged inside the hole groove.

[0010] Compared with the prior art, the utility model has the following beneficial effects: the fixed frame inside the incubator can be driven to rotate by rotating the motor, the telescopic device can adjust the height of the fixed frame, the test tube can be fixed by the fixed frame, and the test tube can be put in and taken out through the tube taking port; the air pressure inside the incubator can be displayed by the internal and external pressure difference display device through the liquid level difference in the tube; the air inside the incubator is automatically regulated by the air regulating device, when the gas inside the incubator is reduced to a certain level, the air pressure pushes the blocking plate down, and the external air enters the incubator from the air inlet pipe, and when the air pressure reaches equilibrium, the spring body continues to push the blocking plate back to block the air inlet pipe. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0012] Figure 2 This is a schematic diagram of the structure of the internal and external pressure difference display device of the utility model;

[0013] Figure 3 This is a schematic diagram of the internal structure of the incubator of the utility model;

[0014] Figure 4 It is a schematic diagram of the telescopic device and the fixing frame structure of the utility model;

[0015] Figure 5 This is a schematic diagram of the structure of the gas regulating device of the utility model;

[0016] Numbers in the figure: 1. incubator; 11. tube taking port; 12. cover; 13. spring shaft; 14. foot support; 2. internal and external pressure difference display device; 21. connecting pipe; 22. liquid in the tube; 23. filter port; 24. connecting port; 3. air regulating device; 31. air inlet pipe; 32. filter port; 33. blocking plate; 34. spring body; 35. spring support plate; 36. sleeve; 4. rotating motor; 41. motor shaft; 42. rotating plate; 5. telescopic device; 51. telescopic rod; 52. support plate; 6. fixing frame; 61. top plate of the frame; 62. support rod; 63. test tube. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0018] In the description of the present invention, it should be noted that the terms "vertical", "up", "down", "horizontal", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0019] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0020] See also Figure 1-5 The utility model provides a technical solution: a cell culture device capable of adjusting gas concentration, comprising a culture box 1, a gas regulating device 3, and a telescopic device 5; an internal and external pressure difference display device 2 is arranged on the side of the culture box 1, a gas regulating device 3 is arranged on the top of the culture box 1, a rotating motor 4 is arranged on the bottom of the culture box 1, a motor shaft 41 is arranged on the rotating motor 4, a rotating plate 42 is arranged on the motor shaft 41, the rotating plate 42 is arranged at the bottom of the culture box 1, a telescopic device 5 is arranged on the rotating plate 42, a fixing frame 6 is arranged on the top of the telescopic device 5, a test tube 63 is arranged on the fixing frame 6, a tube taking port 11 is arranged on the top of the culture box 1, a cover body 12 is arranged on the tube taking port 11, a spring shaft 13 is arranged between the cover body 12 and the tube taking port 11, and a foot support 14 is arranged on the bottom of the culture box 1.

[0021] Furthermore, a connecting tube 21 is provided inside the internal and external pressure difference display device 2, and the connecting tube 21 is in a U-shaped structure. A filter screen port 23 is provided on one end of the connecting tube 21, and a connecting port 24 is provided on the other end of the connecting tube 21. The connecting port 24 is connected to the incubator 1, and a tube liquid 22 is provided inside the connecting tube 21.

[0022] Furthermore, the air regulating device 3 is provided with an air intake pipe 31, and a filter port 32 is provided on the top of the air intake pipe 31. A sleeve 36 is provided inside the air regulating device 3 and sleeved on the outside of the air intake pipe 31. A spring support plate 35 is provided on the bottom of the sleeve 36, and a groove is provided between the spring support plate 35 and the sleeve 36. A plurality of spring bodies 34 are provided on the spring support plate 35, and a blocking plate 33 is provided on the top of the spring body 34. The blocking plate 33 is provided at the bottom of the air intake pipe 31.

[0023] Furthermore, a telescopic rod 51 is provided on the telescopic device 5 , a supporting plate 52 is provided on the telescopic rod 51 , and a fixing frame 6 is provided on the top of the supporting plate 52 .

[0024] Furthermore, the fixing frame 6 is provided with a frame top plate 61 , the frame top plate 61 is provided with a support rod 62 , a hole groove is provided in the middle of the frame top plate 61 , and the test tube 63 is provided inside the hole groove.

[0025] Working principle: by opening the cover body 12, the test tube 63 is inserted into the fixing frame 6 inside the incubator 1 from the tube taking port 11, the test tube 63 is fixed by the fixing frame 6, and the cover body 12 is attached to the tube taking port 11 by the spring shaft 13. The rotating motor 4 can drive the test tube 63 inside the incubator 1 to rotate, so as to facilitate the adjustment of the position of the test tube 63. The rotating fixing frame 6 is inserted into multiple groups of test tubes 63, and the fixing frame 6 can be moved upward by the telescopic device 5, thereby driving the test tube 63 to move upward. The rotating motor 4 adjusts the position of the test tube 63, so as to facilitate the removal of the test tube 63. The internal and external pressure difference display device 2 is set to display the approximate air pressure inside the incubator 1 through the position difference of the liquid 22 in the tube. When the air pressure inside the incubator 1 is too low, the blocking plate 33 cannot block the air inlet pipe 31, and gas is supplied to the inside of the incubator 1 through the air inlet pipe 31.

[0026] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture device with adjustable gas concentration, characterized in that: The invention comprises an incubator (1), an air regulating device (3), and a telescopic device (5); an internal and external pressure difference display device (2) is arranged on the side of the incubator (1); an air regulating device (3) is arranged on the top of the incubator (1); a rotating motor (4) is arranged on the bottom of the incubator (1); a motor shaft (41) is arranged on the rotating motor (4); a rotating plate (42) is arranged on the motor shaft (41); the rotating plate (42) is arranged at the bottom of the incubator (1); a telescopic device (5) is arranged on the rotating plate (42); a fixing frame (6) is arranged on the top of the telescopic device (5); a test tube (63) is arranged on the fixing frame (6); a tube taking port (11) is arranged on the top of the incubator (1); a cover body (12) is arranged on the tube taking port (11); a spring shaft (13) is arranged between the cover body (12) and the tube taking port (11); and a foot support (14) is arranged on the bottom of the incubator (1).

2. A cell culture device with adjustable gas concentration according to claim 1, characterized in that: The internal and external pressure difference display device (2) is provided with a connecting tube (21) in a U-shaped structure, a filter screen port (23) is provided at one end of the connecting tube (21), a connecting port (24) is provided at the other end of the connecting tube (21), the connecting port (24) is connected to the incubator (1), and a tube liquid (22) is provided inside the connecting tube (21).

3. The cell culture device with adjustable gas concentration according to claim 1, characterized in that: The air regulating device (3) is provided with an air intake pipe (31), the top of the air intake pipe (31) is provided with a filter port (32), the air regulating device (3) is provided with a sleeve (36) sleeved on the outside of the air intake pipe (31), the bottom of the sleeve (36) is provided with a spring support plate (35), a slot is provided between the spring support plate (35) and the sleeve (36), a plurality of spring bodies (34) are provided on the spring support plate (35), a blocking plate (33) is provided on the top of the spring body (34), and the blocking plate (33) is provided at the bottom of the air intake pipe (31).

4. The cell culture device with adjustable gas concentration according to claim 1, characterized in that: The telescopic device (5) is provided with a telescopic rod (51), the telescopic rod (51) is provided with a supporting plate (52), and a fixing frame (6) is provided on the top of the supporting plate (52).

5. The cell culture device with adjustable gas concentration according to claim 1, characterized in that: The fixing frame (6) is provided with a frame top plate (61), the frame top plate (61) is provided with a support rod (62), a hole groove is provided in the middle of the frame top plate (61), and the test tube (63) is provided inside the hole groove.