Experimental antibody concentration treatment device

Abstract

The utility model discloses an experimental antibody concentration treatment device, which relates to the field of antibody concentration processing and comprises a box body, a top cover is movably buckled on the top of the box body, an observation window is mounted on the side wall of the box body, a motor is fixedly connected to the bottom wall of the box body, and an output shaft of the motor is in transmission connection with a driving shaft. The outer wall of the driving shaft is fixedly connected with a first stirring blade, the water absorption particles are stirred through rotation of the first stirring blade, a movable groove is prefabricated in the top cover, and the interior of the movable groove is movably connected with a clamping block through a spring. The motor is started to drive the driving shaft to rotate, then the stirring blade I is driven to rotate to turn over and stir water absorption particles placed in the box body so as to improve the water absorption efficiency, and meanwhile, the linkage shaft in transmission connection with the driving shaft synchronously rotates to drive the stirring blade II to rotate to stir antibodies to be concentrated; therefore, the overall concentration efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the field of antibody concentration processing, in particular to an experimental antibody concentration processing device. Background Art

[0002] Antibodies refer to proteins with immune functions produced in human or animal serum due to the invasion of viruses or bacteria. After the antibody is purified, its buffer is generally replaced with PBS for later labeling or other uses. If the concentration is too low, it needs to be concentrated. Concentrated antibodies can adjust the dilution ratio and prepare reagents by themselves. More use means more preparation, less use means less preparation, which can largely ensure the antibody titer and the repeatability of the experiment and the consistency of the results.

[0003] When in use, the existing concentrators usually place a dialysis bag containing an antibody sample together with water-absorbing particles, which absorb moisture from the antibodies to achieve antibody concentration. The existing concentrators also have the following problems when in use: When in use, the sample movement range inside the dialysis bag of the existing concentrator is small, resulting in poor overall water absorption efficiency, which may affect the final concentration effect.

[0004] Therefore, it is necessary to invent an experimental antibody concentration processing device to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to provide an experimental antibody concentration processing device to solve the problem that the existing concentration device proposed in the above background technology has a small sample activity range inside the dialysis bag when in use, resulting in poor overall water absorption efficiency, which may affect the final concentration effect.

[0006] In order to achieve the above-mentioned purpose, the utility model provides the following technical solutions: an experimental antibody concentration processing device, comprising a box, a top cover is movably buckled on the top of the box, an observation window is installed on the side wall of the box, a motor is fixedly connected to the bottom wall of the box, and the output shaft of the motor is transmission-connected to the drive shaft, and a stirring blade 1 is fixedly connected to the outer wall of the drive shaft, and the water-absorbing particles are stirred by the rotation of the stirring blade 1, and also includes:

[0007] The installation assembly is prefabricated with a movable groove inside the top cover, and a clamp block is movably connected to the movable groove through a spring, and the clamp block matches the card slot provided inside the dialysis box, so that the top cover and the dialysis box can achieve synchronous movement, thereby facilitating the taking and placing of the dialysis box;

[0008] The stirring assembly comprises: the bottom of the dialysis box body is rotatably connected to a linkage shaft through a bearing, and the outer wall of the linkage shaft is fixedly connected to a second stirring blade, and the side wall of the lower end of the linkage shaft is fixedly connected to a protrusion, and the protrusion matches the fitting groove arranged at the top of the driving shaft.

[0009] Preferably, the stirring blade 1 is in a "U" shape, and the upper end of the stirring blade 1 is flush with the top of the box body, and water-absorbing particles are placed inside the box body, and the water-absorbing particles are in contact with the stirring blade 1, so as to facilitate the stirring of the water-absorbing particles;

[0010] There is a gap between the stirring blade 1 and the clamping block to prevent the stirring blade 1 from colliding with the clamping block during the rotating stirring process.

[0011] Preferably, the use principle of the dialysis box is the same as that of the dialysis bag, and a sealing cover threadedly connected to the dialysis box is installed on the top of the dialysis box, so that the antibody moisture inside the dialysis box is absorbed by the water-absorbing particles to achieve a concentration effect.

[0012] Preferably, there are two clamping blocks, which are symmetrically arranged with reference to the central axis of the top cover as the axis of symmetry, and the top of the clamping block is in a "T" shape. The outer wall of the "T"-shaped end of the clamping block is in contact with the inner wall of the movable groove and is slidably connected, thereby ensuring the stability of the movable connection between the clamping block and the top cover.

[0013] Preferably, the two ends of the spring are fixedly connected to the inner wall of the movable groove and the side wall of the clamping block respectively, so that the clamping block can tightly clamp the dialysis box under the action of the spring elastic force.

[0014] Preferably, the lower end of the linkage shaft extends beyond the bottom of the dialysis box, and the lower end of the linkage shaft is fixedly connected with two protrusions symmetrically arranged with reference to the linkage shaft as the symmetry axis, so that when the lower end of the linkage shaft is matched with the mating groove set in the end of the drive shaft, the rotation of the drive shaft will drive the rotation of the linkage shaft.

[0015] In the above technical solution, the technical effects and advantages provided by the utility model are:

[0016] 1. The utility model starts the motor to drive the driving shaft to rotate, and then drives the stirring blade 1 to rotate to stir the water-absorbing particles placed inside the box to improve the water absorption efficiency. At the same time, the linkage shaft connected to the driving shaft rotates synchronously to drive the stirring blade 2 to rotate to stir the antibody to be concentrated, so that the antibody water is precipitated faster, thus ensuring the overall concentration efficiency is improved;

[0017] 2. By lifting and opening the top cover, the installation assembly can simultaneously drive the removal of the concentrated dialysis box, so that when the sample is concentrated, the installation and removal are more convenient and quick. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0019] Figure 1 It is a three-dimensional diagram of the overall structure of the utility model;

[0020] Figure 2 It is an exploded view of the overall structure of the utility model;

[0021] Figure 3 It is an exploded view of the box body and top cover (partially cut away) of the utility model;

[0022] Figure 4 For the utility model Figure 3 A magnified effect diagram of the structure at center A;

[0023] Figure 5 It is a three-dimensional diagram of the internal structure of the dialysis box (partially cut away) of the utility model.

[0024] Description of reference numerals:

[0025] 1. Box body; 2. Top cover; 3. Observation window; 4. Motor; 5. Drive shaft; 6. Stirring blade 1; 7. Dialysis box body; 8. Installation assembly; 81. Clamping block; 82. Movable slot; 83. Spring; 84. Slot; 9. Stirring assembly; 91. Linkage shaft; 92. Stirring blade 2; 93. Bump; 94. Fitting slot. DETAILED DESCRIPTION

[0026] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0027] The utility model provides Figure 1-5 The experimental antibody concentration processing device shown includes a box body 1, a top cover 2 is movably fastened on the top of the box body 1, an observation window 3 is installed on the side wall of the box body 1, a motor 4 is fixedly connected to the bottom wall of the box body 1, and the output shaft of the motor 4 is transmission-connected to the drive shaft 5, and the outer wall of the drive shaft 5 is fixedly connected to a stirring blade 6, and the water-absorbing particles are stirred by the rotation of the stirring blade 6, and also includes:

[0028] The mounting assembly 8 is prefabricated with a movable groove 82 inside the top cover 2, and a clamping block 81 is movably connected inside the movable groove 82 through a spring 83, and the clamping block 81 matches the card slot 84 provided inside the dialysis box 7, so that the top cover 2 and the dialysis box 7 can realize synchronous movement, thereby facilitating the taking and placing of the dialysis box 7;

[0029] The stirring assembly 9, the bottom of the dialysis box 7 is rotatably connected to a linkage shaft 91 through a bearing, and a stirring blade 92 is fixedly connected to the outer wall of the linkage shaft 91, and a protrusion 93 is fixedly connected to the side wall of the lower end of the linkage shaft 91, and the protrusion 93 matches the fitting groove 94 set at the top of the driving shaft 5.

[0030] The stirring blade 6 is in a "U" shape, and the upper end of the stirring blade 6 is flush with the top of the box body 1, and water-absorbing particles are placed inside the box body 1, and the water-absorbing particles are in contact with the stirring blade 6, which is convenient for stirring the water-absorbing particles;

[0031] There is a gap between the stirring blade 6 and the clamping block 81 to prevent the stirring blade 6 from colliding with the clamping block 81 during the rotating stirring process.

[0032] The operating principle of the dialysis box 7 is the same as that of the dialysis bag, and a sealing cover threadedly connected to the dialysis box 7 is installed on the top of the dialysis box 7, so that the antibody moisture inside the dialysis box 7 is absorbed by the water-absorbing particles to achieve a concentration effect.

[0033] There are two clamping blocks 81, which are symmetrically arranged with reference to the central axis of the top cover 2 as the symmetry axis, and the top of the clamping block 81 is in a "T" shape. The outer wall of the "T"-shaped end of the clamping block 81 is in contact with the inner wall of the movable groove 82 and is slidably connected, thereby ensuring the stability of the movable connection between the clamping block 81 and the top cover 2.

[0034] The two ends of the spring 83 are fixedly connected to the inner wall of the movable groove 82 and the side wall of the clamping block 81 respectively. Under the elastic force of the spring 83, the clamping block 81 can tightly clamp the dialysis box 7.

[0035] The lower end of the linkage shaft 91 extends beyond the bottom of the dialysis box 7, and the lower end of the linkage shaft 91 is fixedly connected with two protrusions 93 symmetrically arranged with reference to the linkage shaft 91 as the symmetry axis, so that when the lower end of the linkage shaft 91 is matched with the fitting groove 94 set at the end of the driving shaft 5, the rotation of the driving shaft 5 will drive the rotation of the linkage shaft 91.

[0036] Working principle: When using an experimental antibody concentration processing device, firstly, the driving shaft 5 connected to the output shaft of the motor 4 is driven to rotate by starting the motor 4, and then the stirring blade 1 6 fixedly connected to the outer wall of the driving shaft 5 is driven to rotate to stir the water-absorbing particles placed in the box 1 to improve the water absorption efficiency. At the same time, the driving shaft 5 is engaged with the lower end of the linkage shaft 91. During the rotation of the driving shaft 5, the linkage shaft 91 is synchronously rotated and driven to rotate the stirring blade 2 92 fixedly connected to the outer wall of the linkage shaft 91 and located inside the dialysis box 7 to stir the antibody to be concentrated, so that the antibody water is precipitated faster, thereby ensuring the improvement of the overall concentration efficiency;

[0037] After the subsequent concentration is completed, the top cover 2 is lifted and opened, and the dialysis box 7 that has been concentrated and connected with the clamping block 81 in the installation assembly 8 is taken out synchronously under the action of the installation assembly 8. The two clamping blocks 81 are moved in opposite directions so that the ends of the clamping blocks 81 leave the inside of the card slot 84, and the dialysis box 7 can be taken out. The clamping blocks 81 can be moved later so that the clamping blocks 81 are engaged with a new group of dialysis boxes 7 for a new round of concentration processing. The elastic force of the spring 83 makes the matching installation of the clamping blocks 81 and the dialysis box 7 more stable.

[0038] The water-absorbing particles inside the box can be extracted and replaced later by opening the observation window 3 .

[0039] The above only describes some exemplary embodiments of the present invention by way of illustration. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An experimental antibody concentration processing device, comprising a housing (1), characterized in that: The top of the box (1) is movably fastened with a top cover (2), the side wall of the box (1) is provided with an observation window (3), the bottom wall of the box (1) is fixedly connected with a motor (4), the output shaft of the motor (4) is drivingly connected with a drive shaft (5), and the outer wall of the drive shaft (5) is fixedly connected with a stirring blade (6), and further comprises: The mounting assembly (8) is prefabricated with a movable groove (82) inside the top cover (2), and a clamping block (81) is movably connected to the movable groove (82) via a spring (83), and the clamping block (81) matches a clamping groove (84) provided inside the dialysis box (7); A stirring assembly (9), wherein the bottom of the dialysis box (7) is rotatably connected to a linkage shaft (91) via a bearing, and a second stirring blade (92) is fixedly connected to the outer wall of the linkage shaft (91), and a protrusion (93) is fixedly connected to the side wall of the lower end of the linkage shaft (91), and the protrusion (93) matches the engaging groove (94) provided on the top of the driving shaft (5).

2. The experimental antibody concentration processing device according to claim 1, characterized in that: The stirring blade (6) is in a "U" shape, and the upper end of the stirring blade (6) is flush with the top of the box (1), and water-absorbing particles are placed inside the box (1), and the water-absorbing particles are in contact with the stirring blade (6); There is a gap between the stirring blade 1 (6) and the clamping block (81).

3. The experimental antibody concentration processing device according to claim 1, characterized in that: The use principle of the dialysis box (7) is the same as that of the dialysis bag, and a sealing cover threadedly connected to the dialysis box (7) is installed on the top of the dialysis box (7).

4. The experimental antibody concentration processing device according to claim 1, characterized in that: The clamping blocks (81) are provided in two in number. The two clamping blocks (81) are symmetrically arranged with reference to the central axis of the top cover (2) as a symmetric axis, and the top of the clamping block (81) is in a "T" shape. The outer wall of the "T"-shaped end of the clamping block (81) is in contact with and slidably connected to the inner wall of the movable groove (82).

5. The experimental antibody concentration processing device according to claim 1, characterized in that: The two ends of the spring (83) are respectively fixedly connected to the inner wall of the movable groove (82) and the side wall of the clamping block (81).

6. The experimental antibody concentration processing device according to claim 1, characterized in that: The lower end of the linkage shaft (91) extends beyond the bottom of the dialysis box (7), and the lower end of the linkage shaft (91) is fixedly connected to two protrusions (93) that are symmetrically arranged with reference to the linkage shaft (91) as a symmetry axis.

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