Device for detecting omethoate residues in vegetables

Abstract

The utility model provides a device for detecting omethoate residues in vegetables, which belongs to the field of pesticide detection and comprises a storage bin, a detection mechanism is arranged inside the storage bin, and an adjusting component is arranged outside the storage bin. The detection mechanism is arranged, pesticide residues in vegetables can be rapidly detected, meanwhile, the device is small and convenient and can be conveniently used anytime and anywhere, in the detection mechanism, a microwell plate is coated with a specific anti-omethoate antibody, then omethoate in a sample to be detected is combined with the antibody on the microwell plate, and the detection result is accurate. Then adding a secondary antibody marked with enzyme, enabling the secondary antibody to be combined with the antigen compound, and after a substrate is added, carrying out enzyme catalytic reaction to generate visible color change, so as to know the concentration of omethoate in the sample.

Description

Technical Field

[0001] The utility model relates to the field of pesticide detection, in particular to a device for detecting omethoate residues in vegetables. Background Art

[0002] Vegetables are an important part of people's daily diet, and their safety is directly related to human health. In the process of vegetable cultivation, in order to prevent and control pests and diseases, farmers usually use a large amount of pesticides, among which omethoate is a common organophosphorus pesticide. If the pesticide residue in vegetables exceeds the standard, it may cause acute poisoning or even death after human ingestion. Therefore, it is particularly important to detect pesticide residues in vegetables. When detecting omethoate, a detection device is required, but the existing detection device still has the following defects:

[0003] At present, commonly used methods for detecting pesticide residues include gas chromatography, liquid chromatography, etc. Although these methods have high accuracy, the detection process is cumbersome and requires expensive instruments and equipment. In addition, these methods usually need to be carried out under laboratory conditions, which is not conducive to rapid on-site detection.

[0004] Therefore, we made improvements on this and proposed a device for detecting omethoate residues in vegetables. Utility Model Content

[0005] The purpose of the utility model is to address the problem that the commonly used pesticide residue detection methods for an existing omethoate residue detection device in vegetables include gas chromatography, liquid chromatography, etc. Although these methods have high accuracy, the detection process is cumbersome and requires expensive instruments and equipment. In addition, these methods usually need to be carried out under laboratory conditions, which is not conducive to rapid on-site detection.

[0006] In order to achieve the above-mentioned utility model purpose, the utility model provides the following technical solutions:

[0007] A device for detecting omethoate residues in vegetables is provided to improve the above problems.

[0008] The utility model is as follows:

[0009] It comprises a storage bin, a detection mechanism is arranged inside the storage bin, and an adjustment component is arranged outside the storage bin.

[0010] The detection mechanism includes a microplate, an ELISA reader, a vertical groove, a placement frame, a feed hole, a feed tube and a cross bar. The microplate is arranged inside a storage bin, the ELISA reader is arranged at the bottom of the microplate, the vertical groove is penetrated and opened on two opposite side walls of the storage bin, the placement frame is slidably connected to the inside of the storage bin, the feed hole is opened at the bottom of the placement frame, the feed tube is connected and arranged at the bottom of the feed hole, the cross bar is fixedly connected to the side wall of the placement frame, and the cross bar is slidably connected to the vertical groove.

[0011] As a preferred technical solution of the utility model, the adjustment assembly includes a cylinder, a connecting hole, a micromotor, a screw and a connecting block. The cylinder is arranged on the outer side of the storage bin, the connecting hole is opened on the side wall of the cylinder, the micromotor is bolted to the inner top wall of the cylinder, the screw is coaxially arranged at the output end of the micromotor, and the connecting block is threadedly connected to the outside of the screw.

[0012] As a preferred technical solution of the utility model, a circulation hole is opened at the bottom of the storage bin, and the number of the circulation holes is several and evenly distributed along the width direction of the storage bin.

[0013] As a preferred technical solution of the utility model, a limiting rod is fixedly connected to the top of the storage bin, and the side wall of the limiting rod is tightly fitted with the side wall of the cross bar.

[0014] As a preferred technical solution of the present invention, a suction cup is fixedly connected to the bottom of the storage bin, and the number of the suction cups is four and they are respectively located at the four corners of the storage bin.

[0015] As a preferred technical solution of the utility model, two limiting blocks are fixedly connected to the two opposite side walls inside the storage bin, and an insert block is fixedly connected to the two opposite side walls of the microporous plate, and the insert block is located between two adjacent limiting blocks.

[0016] As a preferred technical solution of the present utility model, a timer is fixedly connected to the outer wall of the storage bin.

[0017] As a preferred technical solution of the utility model, a fixed plate is fixedly connected to the outer wall of the storage bin, and the bottom of the cylinder is rotatably connected to the top of the fixed plate.

[0018] As a preferred technical solution of the utility model, a fixing column is coaxially arranged on the top of the cylinder, and a plurality of anti-slip grooves are provided on the outer wall of the fixing column.

[0019] As a preferred technical solution of the utility model, the placement frame and the feed pipe are both made of stainless steel.

[0020] Compared with the prior art, the utility model has the following beneficial effects:

[0021] In the solution of the utility model:

[0022] 1. The utility model can quickly detect pesticide residues in vegetables through the detection mechanism. At the same time, the device is small and convenient, and can be used anytime and anywhere. In the detection mechanism, a specific anti-oxidation chlorpyrifos antibody is coated on a microplate, and then the chlorpyrifos in the sample to be tested is combined with the antibody on the microplate, and then a secondary antibody labeled with an enzyme is added. The secondary antibody will combine with the antigen complex. After the substrate is added, the enzyme catalyzes the reaction to generate a visible color change, and then the concentration of chlorpyrifos in the sample is compared with the standard curve to complete the preliminary detection;

[0023] 2. The utility model can automatically and accurately adjust the height of the placement frame by setting an adjustment component, thereby improving the accuracy of adding the extraction solution and the secondary antibody;

[0024] 3. The arrangement of the placement frame, the feeding hole and the feeding pipe of the utility model can meet the needs of batch testing of a number of vegetable extracts, thus greatly improving the testing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the structure of the device for detecting omethoate residues in vegetables provided by the utility model;

[0026] Figure 2 It is a left view of the device for detecting omethoate residues in vegetables provided by the utility model;

[0027] Figure 3 The utility model provides a device for detecting omethoate residues in vegetables Figure 2 The three-dimensional cross-section view at AA in the middle;

[0028] Figure 4 A front view of the device for detecting omethoate residues in vegetables provided by the utility model;

[0029] Figure 5 The utility model provides a device for detecting omethoate residues in vegetables Figure 4 Plane section at the middle BB;

[0030] Figure 6 The utility model provides a schematic diagram of the structure of the circulation hole of the device for detecting omethoate residues in vegetables.

[0031] Indicated in the figure:

[0032] 1. Storage bin; 2. Detection mechanism; 3. Adjustment assembly; 4. Circulation hole; 5. Limit rod; 6. Suction cup; 7. Limit block; 8. Insert block; 9. Timer; 10. Fixed plate; 11. Fixed column; 12. Anti-slip pattern; 201. Microplate; 202. ELISA reader; 203. Vertical slot; 204. Placement frame; 205. Feeding hole; 206. Feeding tube; 207. Cross bar; 301. Cylinder; 302. Connecting hole; 303. Micromotor; 304. Screw rod; 305. Connecting block. DETAILED DESCRIPTION

[0033] To make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be described clearly and completely in conjunction with the accompanying drawings. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments.

[0034] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the present invention to be protected, but merely represents some embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features and technical solutions in the embodiments may be combined with each other.

[0036] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0037] Preparation of vegetable extract: Take a certain amount of vegetable sample, add an appropriate amount of solvent (such as acetonitrile), oscillate and extract for a certain period of time, then filter the extract to remove solid impurities, and set the filtrate aside.

[0038] Example 1

[0039] like Figure 1-6 As shown, this embodiment provides a device for detecting omethoate residues in vegetables, including a storage compartment 1 , a detection mechanism 2 is disposed inside the storage compartment 1 , and an adjustment component 3 is disposed outside the storage compartment 1 .

[0040] like Figure 3 and Figure 5As shown, the detection mechanism 2 includes a microplate 201, an ELISA reader 202, a vertical slot 203, a placement frame 204, a feed hole 205, a feed tube 206 and a cross bar 207. The microplate 201 is arranged inside the storage bin 1, the ELISA reader 202 is arranged at the bottom of the microplate 201, the vertical slot 203 is penetrated and opened on the two opposite side walls of the storage bin 1, the placement frame 204 is slidably connected to the inside of the storage bin 1, the feed hole 205 is opened at the bottom of the placement frame 204, the feed tube 206 is connected and arranged at the bottom of the feed hole 205, the cross bar 207 is fixedly connected to the side wall of the placement frame 204, the cross bar is slidably connected to the vertical slot, the bottom of the ELISA reader 202 is bolted to the inner bottom wall of the storage bin 1, the number of the feed holes 205 and the feed tube 206 is the same as the number of holes on the microplate 201, the placement frame 2 There are two 04, and several feeding pipes 206 are provided with electromagnetic valves. When in use, the cross bar 207 is slid inside the vertical groove 203, and then the specific anti-oxidation maltose antibody is coated on the microplate 201, and then the extract is placed inside one of the placement frames 204, and then the electromagnetic valve is opened, and the maltose in the sample is combined with the antigen on the microplate 201. After the addition is completed, the placement frame 204 is placed aside, and then the secondary antibody labeled with an enzyme is added to the other placement frame 204, and then the electromagnetic valve is opened. The secondary antibody will combine with the antigen complex. After the substrate is added, the enzyme catalyzes the reaction to generate a visible color change, and then the microplate reader 202 is used to detect the color change in the hole of the microplate 201, and the concentration of maltose in the sample is calculated by the standard curve to complete the detection.

[0041] like Figure 3 As shown, the adjustment component 3 includes a cylinder 301, a connecting hole 302, a micro motor 303, a screw rod 304 and a connecting block 305. The cylinder 301 is arranged on the outer side of the storage bin 1, the connecting hole 302 is opened on the side wall of the cylinder 301, the micro motor 303 is bolted to the inner top wall of the cylinder 301, the screw rod 304 is coaxially arranged at the output end of the micro motor 303, the connecting block 305 is threadedly connected to the outside of the screw rod 304, and the outer wall of the connecting block 305 is detachably connected to the end of the cross bar 207 away from the placement frame 204. The number of the cylinder 301, the micro motor 303, the screw rod 304 and the connecting block 305 is two. , and the two connecting blocks 305 are respectively fixedly connected to the side walls of the two cross bars 207, the top of the screw rod 304 is detachably connected to the output end of the micro motor 303, the micro motor 303 can drive the screw rod 304 to rotate, and the bottom of the screw rod 304 is rotatably connected to the inner bottom wall of the cylinder 301. When detecting omethoate, the worker can start the micro motor 303, so that the output end of the micro motor 303 drives the screw rod 304 to rotate, so that the connecting block 305, the cross bar 207 and the placement frame 204 slide inside the connecting hole, thereby producing a position change, so that the liquid falling through the discharge pipe 206 accurately falls into the hole on the microporous plate 201.

[0042] like Figure 6 As shown, a circulation hole 4 is opened at the bottom of the storage bin 1. There are several circulation holes 4 which are evenly distributed along the width direction of the storage bin 1. When in use, the circulation holes 4 can allow the air inside the storage bin 1 to flow, preventing harmful gases from accumulating inside the storage bin 1.

[0043] like Figure 1 As shown, a limiting rod 5 is fixedly connected to the top of the storage bin 1, and the side wall of the limiting rod 5 fits tightly with the side wall of the cross bar 207. When in use, if the cross bar 207 is to be accurately inserted into the interior of the vertical groove 203, the side wall of the cross bar 207 is fit with the side wall of the limiting rod 5, and then the cross bar 207 is lowered, so that the cross bar 207 accurately enters the vertical groove 203.

[0044] like Figure 1 As shown, the bottom of the storage bin 1 is fixedly connected with a suction cup 6, and there are four suction cups 6, which are respectively located at the four corners of the storage bin 1. When in use, the device can be fixed on an external plate through the suction cup 6 to improve the stability of the device.

[0045] like Figure 5 As shown, two limiting blocks 7 are fixedly connected to the two opposite side walls inside the storage bin 1, and an insert block 8 is fixedly connected to the two opposite side walls of the microporous plate 201. The insert block 8 is located between two adjacent limiting blocks 7. When in use, the microporous plate 201 is placed inside the storage bin 1, and then the insert block 8 is aligned between the two limiting blocks 7, so as to limit the position of the insert block 8, and thus the microporous plate 201 can also be limited.

[0046] like Figure 1 As shown, a timer 9 is fixedly connected to the outer wall of the storage bin 1. When performing testing, each step of incubation needs to be strictly controlled in time. Too long or too short incubation time may lead to errors in the results. The timer 9 can help workers understand the incubation time.

[0047] like Figure 6 As shown, a fixed plate 10 is fixedly connected to the outer wall of the storage bin 1, and the bottom of the cylinder 301 is rotatably connected to the top of the fixed plate 10. There are two fixed plates 10, which are rotatably connected to the bottoms of the two cylinders 301. When in use, the cylinder 301 can be easily rotated by the fixed plate 10, thereby improving convenience.

[0048] like Figure 1 As shown, a fixing column 11 is coaxially arranged on the top of the cylinder 301, and a plurality of anti-slip grooves 12 are provided on the outer wall of the fixing column 11. When in use, the cylinder 301 can be conveniently rotated by the fixing column 11, and the anti-slip grooves 12 can prevent slipping when pinching the fixing column 11, thereby improving convenience.

[0049] Specifically, when the device for detecting omethoate residues in vegetables is used: the solenoid valve is a prior art and is not marked in the drawings of the specification, so it will not be elaborated on here. During the detection, the microplate 201 is coated with antibodies against specific omethoate, and then the cylinder 301 is rotated by the fixed column 11 until the cross bar 207 contacts the limiting rod 5, and then the micromotor 303 is started, and the output end of the micromotor 303 drives the screw rod 304 to rotate, thereby causing the connecting block 305, the cross bar 207 and one of the placement frames 204 to descend along the vertical groove 203, and then the extract is dripped into the interior of the placement frame 204 through an external dropper or a pipette, and then the solenoid valve is opened, and the omethoate in the sample binds to the antibodies on the microplate 201. After the addition is completed, the micromotor 303 is started to reverse its output end, so that the placement frame 204 rises, and then the cylinder 301 is rotated in the opposite direction, and then the secondary antibody marked with the enzyme is added to the inside of another placement frame 204, and then another adjustment component 3 is operated according to the same steps, and then the solenoid valve is opened, the secondary antibody will bind to the antigen complex, and after the substrate is added, the enzyme catalyzes the reaction to generate a visible color change, and then the microplate reader 202 is used to detect the color change in the hole of the microplate 201, and the concentration of omethoate in the sample is compared with the standard curve, so as to complete the preliminary detection and judgment; the standard curve represents the relationship between the color depth and the concentration of omethoate, and the specific preparation method belongs to the well-known technology and will not be repeated here.

[0050] All technical features in this embodiment can be freely combined according to actual needs.

[0051] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present technical solution is within the protection scope of the present invention.

Claims

1. A device for detecting omethoate residues in vegetables, comprising a storage compartment (1), characterized in that: A detection mechanism (2) is arranged inside the storage bin (1), and an adjustment component (3) is arranged outside the storage bin (1); The detection mechanism (2) comprises a microplate (201), an ELISA reader (202), a vertical slot (203), a placement frame (204), a feed hole (205), a feed pipe (206) and a cross bar (207); the microplate (201) is arranged inside the storage bin (1); the ELISA reader (202) is arranged at the bottom of the microplate (201); the vertical slot (203) is penetrated and opened on two opposite side walls of the storage bin (1); the placement frame (204) is slidably connected to the inside of the storage bin (1); the feed hole (205) is opened at the bottom of the placement frame (204); the feed pipe (206) is connected and arranged at the bottom of the feed hole (205); the cross bar (207) is fixedly connected to the side wall of the placement frame (204); and the cross bar (207) is slidably connected to the vertical slot (203); The adjustment assembly (3) comprises a cylinder (301), a communication hole (302), a micromotor (303), a screw (304) and a connection block (305); the cylinder (301) is arranged on an outer side of the storage bin (1); the communication hole (302) is formed on a side wall of the cylinder (301); the micromotor (303) is bolted to an inner top wall of the cylinder (301); the screw (304) is coaxially arranged at an output end of the micromotor (303); and the connection block (305) is threadedly connected to the outside of the screw (304).

2. The device for detecting omethoate residues in vegetables according to claim 1, characterized in that: The bottom of the storage bin (1) is provided with circulation holes (4), and the number of the circulation holes (4) is several and evenly distributed along the width direction of the storage bin (1).

3. The device for detecting omethoate residues in vegetables according to claim 1, characterized in that: A limiting rod (5) is fixedly connected to the top of the storage bin (1), and the side wall of the limiting rod (5) is tightly fitted with the side wall of the cross bar (207).

4. The device for detecting omethoate residues in vegetables according to claim 1, characterized in that: A suction cup (6) is fixedly connected to the bottom of the storage bin (1), and there are four suction cups (6), which are respectively located at four corners of the storage bin (1).

5. The device for detecting omethoate residues in vegetables according to claim 1, characterized in that: Two limiting blocks (7) are fixedly connected to two opposite side walls inside the storage bin (1), and an insert block (8) is fixedly connected to two opposite side walls inside the microporous plate (201), wherein the insert block (8) is located between two adjacent limiting blocks (7).

6. The device for detecting omethoate residues in vegetables according to claim 1, characterized in that: A timer (9) is fixedly connected to the outer wall of the storage bin (1).

7. The device for detecting omethoate residues in vegetables according to claim 2, characterized in that: A fixed plate (10) is fixedly connected to the outer wall of the storage bin (1), and the bottom of the cylinder (301) is rotatably connected to the top of the fixed plate (10).

8. The device for detecting omethoate residues in vegetables according to claim 2, characterized in that: A fixing column (11) is coaxially arranged on the top of the cylinder (301), and a plurality of anti-slip grooves (12) are provided on the outer wall of the fixing column (11).

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