Protein low-temperature purification device

Abstract

The utility model belongs to the technical field of protein purification, and particularly relates to a low-temperature protein purification device. The protein low-temperature purification device comprises a base, a temperature control cylinder and a cover plate, wherein the base is provided with a first phase change energy storage layer, a first heat insulation layer and a first shell from inside to outside; openings are formed in the two ends of the temperature control cylinder, the base is buckled to one end of the temperature control cylinder, and the temperature control cylinder is provided with a second phase change energy storage layer, a second heat insulation layer and a second shell from inside to outside; the cover plate is buckled to the other end of the temperature control cylinder, the base, the temperature control cylinder and the cover plate jointly define a containing cavity, and the chromatographic column is arranged in the containing cavity. Heat in the containing cavity is absorbed through the characteristics of the first phase change energy storage layer and the second phase change energy storage layer, the containing cavity becomes a low-temperature environment, the cover plate, the first heat insulation layer and the second heat insulation layer are arranged, heat exchange between the outside and the interior of the containing cavity is avoided, and the low-temperature environment of the containing cavity is kept. The low-temperature purification cost of the protein is reduced, and the body injury caused by long-term low-temperature environment of an experiment operator is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification, in particular to a low-temperature protein purification device. Background Art

[0002] Protein separation and purification technology is widely used in life science research, biomedicine, clinical diagnosis and other fields. Usually, chromatography columns filled with fillers are used for protein purification. The effect of protein purification has an important impact on the effectiveness, stability and production cost of biological products. For most proteins, especially for genetically engineered recombinant fusion proteins, the thermal stability of proteins is poor. Purification at room temperature can easily lead to loss of protein activity or even protein degradation, resulting in large losses.

[0003] In the prior art, when purifying proteins with poor thermal stability, the chromatography column is usually placed in a cold storage or freezer for purification; however, the cost of using a cold storage is high, which is difficult for smaller laboratories or enterprises to afford. At the same time, being in a low-temperature environment for a long time will have an adverse effect on the health of the experimental operators. Utility Model Content

[0004] The utility model aims to provide a protein low-temperature purification device to solve the problem that the cost of using cold storage for protein purification in the prior art is high; being in a low-temperature environment for a long time will have an adverse effect on the health of the experimental operator, reduce the cost of protein low-temperature purification, and avoid physical damage caused by the experimental operator being in a low-temperature environment for a long time.

[0005] To achieve this purpose, the utility model adopts the following technical solutions:

[0006] Protein low temperature purification device, comprising:

[0007] A base, a temperature control cylinder and a cover plate, wherein the base is provided with a first phase change energy storage layer, a first heat insulation layer and a first shell from the inside out; openings are provided at both ends of the temperature control cylinder, the base is buckled on one end of the temperature control cylinder, and the temperature control cylinder is provided with a second phase change energy storage layer, a second heat insulation layer and a second shell from the inside out; the cover plate is buckled on the other end of the temperature control cylinder, and the base, the temperature control cylinder and the cover plate are jointly arranged to form a containing cavity, and the chromatography column is placed in the containing cavity.

[0008] As an optional technical solution for the protein low-temperature purification device, the first phase change energy storage layer and the second phase change energy storage layer are both configured as phase change energy storage plates, and the phase change energy storage plates contain phase change materials.

[0009] As an optional technical solution for the protein low-temperature purification device, the first thermal insulation layer and the second thermal insulation layer are both configured as vacuum nanoplates.

[0010] As an optional technical solution for the protein low-temperature purification device, the second shell is provided with a first protrusion at one end and a first groove at the other end. The first shell is provided with one of an interlocking groove and an interlocking block to buckle the base to the temperature control cylinder.

[0011] As an optional technical solution for the protein low-temperature purification device, the cover plate is provided with the engaging groove and another of the engaging blocks so as to buckle the cover plate to the temperature control cylinder.

[0012] As an optional technical solution for the protein low-temperature purification device, the first protrusion and the first groove can be embedded so that the multiple temperature control cylinders can be interlocked.

[0013] As an optional technical solution for the protein low-temperature purification device, the cover plate is provided with an injection hole, the base is provided with a sample outlet hole, the injection tube can pass through the injection hole to be connected to the chromatography column, and the sample outlet tube can pass through the sample outlet hole to be connected to the chromatography column.

[0014] As an optional technical solution of the protein low-temperature purification device, the protein low-temperature purification device also includes a fixing frame, which is detachably arranged on the inner side of the base, and a clamp is arranged on the fixing frame, and the clamp is used to clamp the chromatography column.

[0015] As an optional technical solution for the protein low-temperature purification device, a support column is arranged on the outside of the base, and a plurality of support columns are arranged at intervals around the central axis of the base. The support columns are rotatably connected to the base, and the free ends of the support columns can abut or disengage from the base as they rotate.

[0016] As an optional technical solution for the protein low-temperature purification device, a handle is provided on the outer side of the cover plate.

[0017] Beneficial effects of the utility model:

[0018] The utility model provides a protein low-temperature purification device, including a base, a temperature control tube and a cover plate. The base is provided with a first phase change energy storage layer, a first heat insulation layer and a first shell from the inside out; openings are provided at both ends of the temperature control tube, the base is buckled to one end of the temperature control tube, and the temperature control tube is provided with a second phase change energy storage layer, a second heat insulation layer and a second shell from the inside out; the cover plate is buckled to the other end of the temperature control tube, and the base, the temperature control tube and the cover plate are jointly arranged to form a receiving cavity, and the chromatography column is placed in the receiving cavity. The heat in the receiving cavity is absorbed by utilizing the inherent characteristics of the first phase change energy storage layer and the second phase change energy storage layer, so that the receiving cavity becomes a low-temperature environment, and the cover plate, the first heat insulation layer and the second heat insulation layer are arranged to avoid heat exchange between the outside and the receiving cavity, so as to maintain the low-temperature environment of the receiving cavity. The cost of low-temperature purification of proteins is reduced, and the body damage caused by the long-term exposure of experimental operators to a low-temperature environment is avoided. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0020] Figure 1 It is a structural schematic diagram of a protein low-temperature purification device provided in an embodiment of the utility model;

[0021] Figure 2 yes Figure 1 The enlarged view of point A in the middle;

[0022] Figure 3 yes Figure 1 The enlarged view of point B in the middle;

[0023] Figure 4 This is a schematic diagram of the cover structure of the protein low-temperature purification device provided by the embodiment of the utility model;

[0024] Figure 5 It is a structural schematic diagram of a temperature control cylinder of a protein low-temperature purification device provided in an embodiment of the utility model;

[0025] Figure 6 It is a partial structural schematic diagram of a protein low-temperature purification device provided in an embodiment of the utility model.

[0026] In the figure:

[0027] 10. base; 11. first phase change energy storage layer; 12. first heat insulation layer; 13. first shell; 14. sample outlet;

[0028] 20. Temperature control tube; 21. Second phase change energy storage layer; 22. Second heat insulation layer; 23. Second shell;

[0029] 30. Cover plate; 31. Injection hole; 32. Handle;

[0030] 41. first protrusion; 42. first groove; 43. fitting groove; 44. fitting block;

[0031] 50. Fixing frame; 51. Clamp;

[0032] 60. Support column. DETAILED DESCRIPTION

[0033] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention, rather than all structures, are shown in the accompanying drawings.

[0034] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0036] In the description of this embodiment, the terms "upper", "lower", "right", etc., are based on the directions or positions shown in the drawings, and are only for the convenience of description and simplified operation, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used to distinguish in the description and have no special meaning.

[0037] In the prior art, when purifying proteins with poor thermal stability, the chromatography column is usually placed in a cold storage or freezer for purification; however, the cost of using a cold storage is high, which is difficult for smaller laboratories or enterprises to afford. At the same time, being in a low-temperature environment for a long time will have an adverse effect on the health of the experimental operators.

[0038] To solve the above problems, this embodiment provides a protein low temperature purification device, see Figure 1 and Figure 5, including a base 10, a temperature control tube 20 and a cover plate 30. The base 10 is provided with a first phase change energy storage layer 11, a first heat insulation layer 12 and a first shell 13 from the inside to the outside; the temperature control tube 20 is provided with openings at both ends, the base 10 is buckled at one end of the temperature control tube 20, and the temperature control tube 20 is provided with a second phase change energy storage layer 21, a second heat insulation layer 22 and a second shell 23 from the inside to the outside; the cover plate 30 is buckled at the other end of the temperature control tube 20, and the base 10, the temperature control tube 20 and the cover plate 30 are jointly arranged to form a receiving cavity, and the chromatography column is placed in the receiving cavity. The heat in the receiving cavity is absorbed by the characteristics of the first phase change energy storage layer 11 and the second phase change energy storage layer 21, so that the receiving cavity becomes a low-temperature environment, and the cover plate 30, the first heat insulation layer 12 and the second heat insulation layer 22 are arranged to avoid heat exchange between the outside and the receiving cavity, so as to maintain the low-temperature environment of the receiving cavity. The cost of low-temperature purification of proteins is reduced, and the body damage caused by long-term exposure of experimental operators to low-temperature environments is avoided.

[0039] Specifically, the first phase change energy storage layer 11 and the second phase change energy storage layer 21 are both configured as phase change energy storage plates, and phase change materials are contained inside the phase change energy storage plates. Phase change material is a thermal energy storage material. When the form of the phase change material is transformed from solid to liquid, it can absorb the heat in the accommodating cavity to reduce the temperature in the accommodating cavity. Exemplarily, before low-temperature purification of protein, the base 10 and the temperature control cylinder 20 are first placed in a refrigerator or freezer to freeze the phase change material into a solid state. During the low-temperature purification of protein, the phase change material continuously absorbs the heat of the accommodating cavity and transforms from a solid state to a liquid state to continuously provide a low-temperature environment for low-temperature purification of protein, thereby avoiding loss of activity or degradation of the protein and causing loss. Specifically, the phase change material has been disclosed in the patent with publication number CN2779325Y published by the State Intellectual Property Office of China, and will not be repeated here.

[0040] Further, the first thermal insulation layer 12 and the second thermal insulation layer 22 are both set as vacuum nanoplates. The vacuum nanoplate can isolate the first phase change energy storage layer 11 and the second phase change energy storage layer 21 from heat exchange with the external environment through the first shell 13 and the second shell 23, so that the low temperature environment is always maintained in the accommodation cavity. Specifically, the vacuum nanoplate can be set individually or in multiples, which is not limited here. Specifically, the thickness of the single-layer vacuum nanoplate is 10mm-40mm.

[0041] Further, see Figure 2 and Figure 3, a first protrusion 41 is provided at one end of the second shell 23, and a first groove 42 is provided at the other end, and the first shell 13 is provided with one of the fitting groove 43 and the fitting block 44, so as to buckle the base 10 to the temperature control cylinder 20. The cover plate 30 is provided with the other of the fitting groove 43 and the fitting block 44, so as to buckle the cover plate 30 to the temperature control cylinder 20. In this embodiment, the temperature control cylinder 20 is configured as a cylindrical cylinder, and a circular fitting groove 43 is provided on the end of the first shell 13, and a circular fitting block 44 is provided on the cover plate 30. The temperature control cylinder 20 and the base 10 are fitted together by the first protrusion 41 and the fitting groove 43, which can prevent the temperature control cylinder 20 from shaking on the base 10, increase the connection stability, and compared with the direct docking of the temperature control cylinder 20 and the base 10, the heat exchange at the joint is reduced, and the insulation effect in the accommodating cavity is improved. Furthermore, the abutting surfaces of the first protrusion 41 , the first groove 42 , the fitting groove 43 and the fitting block 44 may be provided with rubber pads or silicone pads to seal the joints and reduce heat exchange.

[0042] Further, see Figure 4 A handle 32 is provided on the outside of the cover plate 30. It is convenient to place the cover plate 30 on the temperature control cylinder 20 or open the cover plate 30. Specifically, the handle 32 can be set as a pull ring or a pull groove.

[0043] Furthermore, the first protrusion 41 and the first groove 42 can be engaged with each other, so that the multiple temperature control cylinders 20 can be interlocked. The multiple temperature control cylinders 20 can be interlocked and interlocked with each other to adapt to chromatography columns of different lengths, thereby increasing the versatility of the protein low-temperature purification device.

[0044] Further, see Figure 4 and Figure 6 The cover plate 30 is provided with an injection hole 31, and the base 10 is provided with an outlet hole 14. The injection tube can pass through the injection hole 31 to communicate with the chromatography column, and the outlet tube can pass through the outlet hole 14 to communicate with the chromatography column. During the low-temperature purification of proteins, it is necessary to add purification buffer and the like into the chromatography column to assist the low-temperature purification of proteins; when the purification is completed, the purified protein sample liquid is led out through the outlet tube to avoid the disappearance of the low-temperature environment of the containing chamber when the cover plate 30 is opened to add reagents and take out the sample liquid.

[0045] In this embodiment, the injection hole 31 is arranged at the edge of the cover plate 30 and is arranged as a circular notch, and the sample outlet hole 14 is arranged at the geometric center of the base 10. In actual use, the circular notch can be placed on the side away from the chromatography column to reduce the heat exchange at the injection hole 31. Furthermore, flexible sealing members, such as rubber rings or silicone rings, can be arranged between the injection hole 31 and the injection tube and between the sample outlet hole 14 and the sample outlet tube to reduce the heat exchange at the injection hole 31 and the sample outlet hole 14.

[0046] Furthermore, the protein low-temperature purification device also includes a fixed frame 50, which is detachably arranged on the inner side of the base 10, and a clamp 51 is arranged on the fixed frame 50, and the clamp 51 is used to clamp the chromatography column. Specifically, the fixed frame 50 and the base 10 can be screwed or plugged. When the base 10 is placed in a refrigerator or freezer, the fixed frame 50 is removed to save space in the refrigerator or freezer. In this embodiment, the clamp 51 is configured as an arc-shaped clamp with a fixed shape. In other embodiments, the clamp 51 can also be configured as a bolt-locked clamp to adapt to chromatography columns of different diameters, thereby increasing the versatility of the protein low-temperature purification device. The clamp 51 and the fixed frame 50 can be welded or glued, which is not limited here.

[0047] Further, see Figure 6 , a support column 60 is arranged on the outside of the base 10, and a plurality of support columns 60 are arranged at intervals around the central axis of the base 10. The support columns 60 are rotatably connected to the base 10, and the free ends of the support columns 60 can abut or disengage with the base 10 as they rotate. In this embodiment, four support columns 60 are arranged, and the four support columns 60 can be folded on the outside of the base 10, so that the base 10 can be placed in a refrigerator or freezer. The support columns 60 and the base 10 can be connected by hinges or rotating shafts. Since the rotatably connected and folded support columns 60 are common structures in the mechanical field, they are not described or listed here.

[0048] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For those skilled in the art, various obvious changes, readjustments and substitutions can be made without departing from the scope of protection of the present invention. It is not necessary and impossible to list all implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the scope of protection of the claims of the present invention.

Claims

1. A protein low temperature purification device, characterized in that: include: A base (10), wherein the base (10) is provided with a first phase-change energy storage layer (11), a first heat insulation layer (12) and a first shell (13) from the inside to the outside; A temperature control cylinder (20), wherein both ends of the temperature control cylinder (20) are provided with openings, the base (10) is buckled to one end of the temperature control cylinder (20), and the temperature control cylinder (20) is provided with a second phase change energy storage layer (21), a second heat insulation layer (22) and a second shell (23) from the inside to the outside; A cover plate (30), wherein the cover plate (30) is buckled onto the other end of the temperature control tube (20), and the base (10), the temperature control tube (20) and the cover plate (30) are jointly arranged to form a receiving cavity, and the chromatography column is placed in the receiving cavity.

2. The protein low temperature purification device according to claim 1, characterized in that: The first phase-change energy storage layer (11) and the second phase-change energy storage layer (21) are both configured as phase-change energy storage plates, wherein phase-change material is contained in the phase-change energy storage plates.

3. The protein low temperature purification device according to claim 1, characterized in that: The first heat insulation layer (12) and the second heat insulation layer (22) are both configured as vacuum nanoplates.

4. The protein low-temperature purification device according to claim 1, characterized in that: The second shell (23) is provided with a first protrusion (41) at one end and a first groove (42) at the other end, and the first shell (13) is provided with one of an engaging groove (43) and an engaging block (44) to buckle the base (10) to the temperature control cylinder (20).

5. The protein low-temperature purification device according to claim 4, characterized in that: The cover plate (30) is provided with the other of the engaging groove (43) and the engaging block (44) so ​​as to buckle the cover plate (30) to the temperature control cylinder (20).

6. The protein low temperature purification device according to claim 4, characterized in that: The first protrusion (41) and the first groove (42) can be engaged with each other so that a plurality of the temperature control cylinders (20) can be buckled with each other.

7. The protein low temperature purification device according to any one of claims 1 to 6, characterized in that: The cover plate (30) is provided with a sample injection hole (31), and the base (10) is provided with a sample outlet hole (14); a sample injection tube can pass through the sample injection hole (31) to communicate with the chromatography column, and a sample outlet tube can pass through the sample outlet hole (14) to communicate with the chromatography column.

8. The protein low temperature purification device according to any one of claims 1 to 6, characterized in that: The protein low-temperature purification device further comprises a fixing frame (50), wherein the fixing frame (50) is detachably arranged on the inner side of the base (10), and a clamp (51) is arranged on the fixing frame (50), and the clamp (51) is used to clamp the chromatography column.

9. The protein low temperature purification device according to any one of claims 1 to 6, characterized in that: A support column (60) is arranged on the outside of the base (10), and a plurality of the support columns (60) are arranged at intervals around the central axis of the base (10). The support columns (60) are rotatably connected to the base (10), and the free ends of the support columns (60) can abut against or disengage from the base (10) as they rotate.

10. The protein low temperature purification device according to any one of claims 1 to 6, characterized in that: A handle piece (32) is arranged on the outer side of the cover plate (30).

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