Built-in sample preservation mechanism of liquid nitrogen lifting pot

By designing a sample storage mechanism in the liquid nitrogen lifting pot, the problem of floating and stacking of sample collection tubes is solved, uniform cooling and efficient storage of samples are achieved, and the low-temperature storage and transportation needs of a large number of biological samples are met.

CN222366924UActive Publication Date: 2025-01-21NINGXIA MEDICAL UNIV
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Patent Information

Application Number
CN202421211777.8
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-30
Publication Date
2025-01-21
Estimated Expiration
2034-05-30

AI Technical Summary

Technical Problem

During the low-temperature storage and transportation of biological samples, existing liquid nitrogen lifting pots cannot effectively prevent the sample collection tube from floating, resulting in the sample being unable to be uniformly immersed by liquid nitrogen and quickly frozen. In the case of too many samples, the tube body stacking is serious and cannot meet a large number of samples. low temperature storage requirements.

Method used

A built-in sample storage mechanism in a liquid nitrogen lifting pot is designed, including a support column, a protective cover, a mounting rack and a support mechanism. Through the design of the mounting holes and support mechanism, the sample collection tubes can be placed in an orderly manner and prevented from floating, ensuring that the sample is completely uniform. Submerged in liquid nitrogen and flash frozen.

Benefits of technology

It achieves stable placement and uniform cooling of sample collection tubes, avoids sample floating and stacking phenomena, meets the needs for low-temperature storage and transportation of a large number of biological samples, and improves the efficiency and effect of sample preservation.

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Abstract

The utility model discloses a liquid nitrogen lifting pot built-in sample preservation mechanism which is characterized in that the outer wall of a supporting column is sleeved with a protective cover and a mounting frame, the mounting frame is located below the protective cover, a plurality of mounting holes used for mounting sample collecting pipes are formed in the mounting frame, and a supporting mechanism is arranged on the supporting column; the supporting mechanism is located below the mounting frame after being unfolded and makes contact with the bottom face of the mounting frame. A positioning mechanism is arranged on the supporting column and connected with the protective cover. According to the utility model, the sample collecting pipes can be orderly placed in the mounting holes, and the protective cover can just press the sample collecting pipes to prevent the sample collecting pipes from floating, so that all samples are completely and uniformly immersed in liquid nitrogen and are quickly frozen. After being unfolded, the supporting mechanism is located below the mounting frame and makes contact with the bottom face of the mounting frame, it is guaranteed that the structural temperature of the mounting frame cannot be loosened when the mounting frame enters liquid nitrogen, the cooling effect of the sample collecting pipe is optimal, and the positioning mechanism is arranged on the supporting column to limit the position of the protective cover and prevent the protective cover from moving in the liquid nitrogen and cannot press the sample collecting pipe.
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Description

Technical Field

[0001] The utility model relates to the technical field of quick freezing and transport storage of biological samples, in particular to a liquid nitrogen lifting pot with a built-in sample preservation mechanism. Background Art

[0002] In the process of medical treatment and scientific research, the scope of application of quick freezing and transportation storage of biological samples is increasing. However, in the process of collecting, storing and transporting a large number of biological samples for epidemiological surveys, it is impossible to allocate liquid nitrogen tanks to each surveyed area, and most standard liquid nitrogen tanks are imported products from foreign companies such as Europe and the United States. Such tanks are not only bulky but also expensive. It seems to be overkill for short-term storage and transportation of samples. At present, small liquid nitrogen barrels (such as 2-liter liquid nitrogen lifting pots) are used in China. They are made of double-layer stainless steel materials and filled with polyester foam anti-low temperature fillers in the middle. They can preserve biological samples at low temperatures for four hours, which can meet the requirements of rapid low-temperature quick freezing and transportation within four hours. However, when storing biological samples after collection, the biological sample collection tube will float on the liquid surface in liquid nitrogen and too many samples will cause the tube body to stack, and it is impossible to achieve that all samples are completely and evenly immersed in liquid nitrogen and quick frozen. The stainless steel biological sample tube rack can place the sample collection tube in a standardized manner and has gravity, but it is generally square and has a large volume and area, and cannot be placed in a liquid nitrogen lifting pot. Utility Model Content

[0003] The purpose of the utility model is to overcome the problems raised in the above-mentioned background technology, and provide a liquid nitrogen lifting pot with a built-in sample preservation mechanism, which is suitable for different specifications and models and can be placed in a small liquid nitrogen special barrel, thereby meeting the conditions for low-temperature storage and transportation of a large number of biological samples in a short period of time, and achieving the results of low cost and high efficiency.

[0004] The purpose of the utility model is mainly achieved through the following technical solutions:

[0005] The liquid nitrogen lifting pot has a built-in sample preservation mechanism, including a support column, the outer wall of the support column is covered with a protective cover and a mounting frame, and the mounting frame is located below the protective cover, and the mounting frame is provided with a number of mounting holes for installing sample collection tubes, and the support column is provided with a supporting mechanism, which is located below the mounting frame and in contact with the bottom surface of the mounting frame after unfolding; the support column is provided with a positioning mechanism, and the positioning mechanism is connected to the protective cover. At present, when storing biological samples after collection, the collection tube is directly placed in liquid nitrogen. Due to factors such as gravity, the biological sample collection tube will float on the liquid surface in liquid nitrogen and cannot be effectively cooled. At the same time, the biological sample collection tube is randomly scattered, and too many samples will cause the tube body to stack, and it is impossible to achieve that all samples are completely and evenly immersed in liquid nitrogen and quickly frozen. There are also tube racks designed for placement, but they are generally square and have a large volume and area, and cannot be placed in a liquid nitrogen lifting pot. In order to solve the above problems, this scheme designs a liquid nitrogen lifting pot with built-in sample preservation mechanism, by sleeved with a protective cover and a mounting frame on the outer wall of the support column, and the mounting frame is located below the protective cover, and the mounting frame is provided with a number of mounting holes for installing sample collection tubes, so that the sample collection tubes can be placed in the mounting holes in an orderly manner, and the protective cover can just press the sample collection tubes to prevent them from floating, so that all samples are completely and evenly immersed in liquid nitrogen and quickly frozen. In addition, in order to ensure that the structure is more stable, a supporting mechanism is provided on the support column, and after the supporting mechanism is unfolded, it is located below the mounting frame and contacts the bottom surface of the mounting frame, ensuring that the structural temperature of the mounting frame will not loosen when entering the liquid nitrogen, so that the cooling effect of the sample collection tube is optimal, and a positioning mechanism is provided on the support column, and the positioning mechanism is connected to the protective cover to limit the position of the protective cover to prevent it from moving in the liquid nitrogen and failing to press the sample collection tube.

[0006] Furthermore, the support mechanism includes a transverse support rod and an oblique support rod, and one end of the transverse support rod is connected to the support column and can rotate around the connection, and the oblique support rod includes an oblique support rod 1 and an oblique support rod 2, the oblique support rod 1 is connected to one end of the transverse support rod away from the support column and can rotate around the connection, the oblique support rod 1 and the oblique support rod 2 are connected and can rotate around the connection, and the other end of the oblique support rod 2 is connected to the support column and can rotate around the connection. By unfolding the transverse support rod to a horizontal state, the oblique support rod 1 and the oblique support rod 2 are unfolded to the same straight line, so that the transverse support rod, the oblique support rod 1, the oblique support rod 2 and the support column form a triangular structure, which can effectively support the mounting frame, and the transverse support rod is in contact with the bottom of the mounting frame, so that the mounting frame can remain stable.

[0007] Furthermore, in order to make the oblique support rod 1 and the oblique support rod 2 more stable after being unfolded, a connecting pin is provided on the oblique support rod 2, and the connecting pin passes through the oblique support rod 1 and the oblique support rod 2 at the same time, and the end of the connecting pin is bent and extended, and a locking hole is provided on the oblique support rod 1, and the end of the connecting pin can be inserted into the locking hole. The oblique support rod 1 and the oblique support rod 2 can both rotate around the connecting pin, and the connecting pin is designed to have a certain length, and it can have a certain movement space along its own axis. After the oblique support rod 1 and the oblique support rod 2 rotate to the same straight line, the connecting pin is moved, and the bent end is inserted into the locking hole to lock the positions of the oblique support rod 1 and the oblique support rod 2, so that the support is more stable, and when it is necessary to rotate, the end of the locking pin can be pulled out from the locking hole.

[0008] Furthermore, the positioning mechanism includes a positioning rod bent at 90°, one end of the positioning rod is fixed to the support column, and the other end is bent upward to form a positioning hook. A through hole is provided on the protective cover, and the support column passes through the through hole. A through groove and an arc groove are provided on the protective cover, and the through groove is connected to the through hole, and the other end of the through groove is connected to the arc groove, and the positioning rod can pass through the through groove and the arc groove. The positioning mechanism itself is fixed in position. When installing the protective cover, the protective cover is put on along the support column, and the positioning rod is passed through the space through the through groove. At this time, the length of the positioning hook exceeds the through groove, that is, the main body of the positioning rod is located above the protective cover, and the positioning hook is located below the protective cover. The protective cover is rotated to move the rod body of the positioning rod from the through groove into the arc groove, so that the protective cover can be positioned. It will not move when used in the pot, ensuring the suppression of the sample collection tube.

[0009] Furthermore, in order to facilitate operation, an end cover is provided at the top of the support column, and a handle is provided on the end cover. The handle is convenient for operators to take and put the entire mechanism.

[0010] In summary, compared with the prior art, the present invention has the following beneficial effects:

[0011] (1) The utility model can place the sample collection tubes in the mounting holes in an orderly manner, and the protective cover can just press the sample collection tubes to prevent them from floating, so that all samples are completely and evenly immersed in liquid nitrogen and quickly frozen. In order to ensure a more stable structure, a support mechanism is provided on the support column. After the support mechanism is unfolded, it is located below the mounting frame and contacts the bottom surface of the mounting frame to ensure that the structural temperature of the mounting frame will not loosen when entering the liquid nitrogen, so that the cooling effect of the sample collection tube is optimal. A positioning mechanism is provided on the support column, and the positioning mechanism is connected to the protective cover to limit the position of the protective cover to prevent it from moving in the liquid nitrogen and failing to press the sample collection tube.

[0012] (2) The utility model can effectively support the mounting frame by forming a triangular structure with the transverse support rod, the first oblique support rod, the second oblique support rod and the support column. The transverse support rod is in contact with the bottom of the mounting frame, so that the mounting frame can remain stable.

[0013] (3) The combination of the through groove and the arc groove of the utility model enables the rod body of the positioning rod to enter the arc groove from the through groove, thereby realizing the positioning of the protective cover. The protective cover will not move when used in the pot, thereby ensuring the pressing of the sample collection tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The drawings described herein are used to provide a further understanding of the embodiments of the present utility model, constitute a part of this application, and do not constitute a limitation of the embodiments of the present utility model. In the drawings:

[0015] Figure 1 It is the front view of the utility model.

[0016] Figure 2 for Figure 1 A partial schematic diagram of .

[0017] Figure 3 Schematic diagram of the expanded structure of the support mechanism.

[0018] Figure 4 for Figure 3 A partial schematic diagram of .

[0019] The names corresponding to the reference numerals in the accompanying drawings are:

[0020] 1-handle, 2-end cover, 3-protective cover, 4-sample collection tube, 5-mounting frame, 6-support column, 7-positioning rod, 8-arc groove, 9-through groove, 10-transverse support rod, 11-oblique support rod 1, 12-oblique support rod 2, 13-locking hole, 14-connecting pin. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the utility model more clearly understood, the utility model is further described in detail below in conjunction with embodiments and drawings. The schematic specific implementation methods of the utility model and their descriptions are only used to explain the utility model and are not intended to limit the utility model.

[0022] like Figures 1 to 4As shown, this embodiment includes a support column 6, an end cap 2 is provided at the top of the support column 6, and a handle 1 is provided on the end cap 2. The handle 1 is convenient for operators to take and place the entire mechanism. A protective cover 3 and a mounting frame 5 are sleeved on the outer wall of the support column 6, and the mounting frame 5 is located below the protective cover 3. The mounting frame 5 is provided with a plurality of mounting holes for mounting the sample collection tube 4. The mounting holes are convenient for placing the sample collection tube in order. The protective cover 3 can just press the sample collection tube to prevent it from floating, so that all samples are completely and evenly immersed in liquid nitrogen and quickly frozen. The protective cover 3 should cover all the mounting holes.

[0023] In order to ensure that the structure is more stable, a supporting mechanism is provided on the supporting column 6. After the supporting mechanism is unfolded, it is located below the mounting frame 5 and contacts with the bottom surface of the mounting frame 5, ensuring that the structural temperature of the mounting frame 5 will not loosen when entering the liquid nitrogen, so that the cooling effect of the sample collection tube is optimal. The supporting mechanism includes a transverse support rod 10 and an oblique support rod, and one end of the transverse support rod 10 is connected to the supporting column 6 and can rotate around the connection. The oblique support rod includes an oblique support rod 11 and an oblique support rod 2 12. The oblique support rod 1 11 is connected to one end of the transverse support rod 10 away from the supporting column 6 and can rotate around the connection. The oblique support rod 1 11 is connected to the oblique support rod 2 12 and can rotate around the connection. The other end of the oblique support rod 2 12 is connected to the support column 6 and can rotate around the connection. By unfolding the transverse support rod 10 to a horizontal state, and unfolding the oblique support rod 1 11 and the oblique support rod 2 12 to the same straight line, the transverse support rod 10, the oblique support rod 1 11, the oblique support rod 2 12 and the support column 6 form a triangular structure, which can effectively support the mounting frame 5. The transverse support rod 10 and the bottom of the mounting frame 5 are in contact, so that the mounting frame 5 can remain stable. At the same time, in order to facilitate the overall structure to be compact, a mounting groove can be formed in the outer wall of the support column 6. The folded transverse support rod 10, the oblique support rod 1 11 and the oblique support rod 2 12 can all be stored in the mounting groove without affecting the movement of the mounting frame 5 on the support column 6. It is best to set the support mechanism symmetrically along the support column 6 to make the support more stable.

[0024] The oblique support rod 12 is provided with a connecting pin 14, which passes through the oblique support rod 1 1 and the oblique support rod 1 12 at the same time. The end of the connecting pin 14 is bent and extended. The oblique support rod 1 1 is provided with a locking hole 13, and the end of the connecting pin 14 can be inserted into the locking hole 13. The oblique support rod 1 11 and the oblique support rod 2 12 can both rotate around the connecting pin 14, and the connecting pin 14 is designed to have a certain length, and it can have a certain movement space along its own axis. After the oblique support rod 1 11 and the oblique support rod 2 12 rotate to the same straight line, the connecting pin 14 is moved, and the bent end is inserted into the locking hole 13 to lock the positions of the oblique support rod 1 11 and the oblique support rod 2 12, so that the support is more stable. When it is necessary to rotate, the end of the locking pin 14 can be pulled out from the locking hole 13.

[0025] A positioning mechanism is provided on the support column 6, and the positioning mechanism is connected to the protective cover 3 to limit the position of the protective cover 3 to prevent it from moving in the liquid nitrogen and failing to press the sample collection tube. The positioning mechanism includes a positioning rod 7 bent at 90°, one end of the positioning rod 7 is fixed to the support column 6, and the other end is bent upward to form a positioning hook. A through hole is provided on the protective cover 3, and the support column 6 passes through the through hole. A through groove 9 and an arc groove 8 are provided on the protective cover 3, and the through groove 9 is connected to the through hole, and the other end of the through groove 9 is connected to the arc groove 8, and the positioning rod 7 can pass through the through groove 9 and the arc groove 8. The positioning mechanism itself is fixed in position. When installing the protective cover 3, the protective cover 3 is put on along the support column 6. After the positioning rod 7 is passed through the space by the through slot 9, the length of the positioning hook exceeds the through slot 9, that is, the main body of the positioning rod 7 is located above the protective cover 3, and the positioning hook is located below the protective cover 3. The protective cover 3 is rotated, and the rod body of the positioning rod 7 enters the arc groove 8 from the through slot 9, so that the protective cover 3 can be positioned. It will not move when used in the pot, ensuring the suppression of the sample collection tube. In order to stabilize the protective cover 3, the positioning mechanism can be symmetrically arranged along the support column 6, and the corresponding through slots 9 and arc grooves 8 are also symmetrically arranged.

[0026] The assembled utility model is placed in a 2-liter liquid nitrogen lifting pot filled with liquid nitrogen, and the lid of the 2-liter liquid nitrogen lifting pot is covered, thus completing the whole process of stably storing the sample collection tube using the liquid nitrogen lifting pot built-in sample storage rack of the utility model. Moreover, the scheme can be designed as a multi-layer structure according to needs, which can accommodate more sample collection tubes.

[0027] In laboratories, hospitals, research institutes and other places, liquid nitrogen tanks are undoubtedly indispensable devices for sample storage. As an important accessory of the liquid nitrogen tank, the cryostat is used to store cryotubes containing biological specimens in the liquid nitrogen container. The mechanism designed in this scheme has gravity and a lid on the tube rack, which can meet the requirements of standard collection of biological sample collection tubes; reduce the possibility of tubes floating or accumulating on liquid nitrogen, resulting in the inability to quickly freeze the samples and thus affect the subsequent experimental results; it is also convenient for sample storage and retrieval, summarization and organization, and effectively reduces liquid nitrogen loss and improves work efficiency; through the layered and gridded design, more samples can be stored in the liquid nitrogen tank to maximize space utilization. Each layer makes the cryotubes neat and orderly, which is convenient for identifying and taking samples and ensuring safe retrieval.

[0028] The above specific implementation methods further illustrate the purpose, technical solutions and beneficial effects of the utility model in detail. It should be understood that the above are only specific implementation methods of the utility model and are not used to limit the protection scope of the utility model. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the utility model should be included in the protection scope of the utility model.

Claims

1. The liquid nitrogen lifting pot has a built-in sample storage mechanism, which is characterized by: The invention comprises a support column (6), the outer wall of the support column (6) is covered with a protective cover (3) and a mounting frame (5), and the mounting frame (5) is located below the protective cover (3), and a plurality of mounting holes for mounting a sample collection tube (4) are provided on the mounting frame (5), and a support mechanism is provided on the support column (6), and the support mechanism is located below the mounting frame (5) and contacts the bottom surface of the mounting frame (5) after being unfolded; a positioning mechanism is provided on the support column (6), and the positioning mechanism is connected to the protective cover (3).

2. The liquid nitrogen lifting pot with built-in sample storage mechanism according to claim 1, characterized in that: The support mechanism comprises a transverse support rod (10) and an oblique support rod, wherein one end of the transverse support rod (10) is connected to the support column (6) and can rotate around the connection, and the oblique support rod comprises an oblique support rod 1 (11) and an oblique support rod 2 (12), wherein the oblique support rod 1 (11) is connected to one end of the transverse support rod (10) away from the support column (6) and can rotate around the connection, the oblique support rod 1 (11) is connected to the oblique support rod 2 (12) and can rotate around the connection, and the other end of the oblique support rod 2 (12) is connected to the support column (6) and can rotate around the connection.

3. The liquid nitrogen lifting pot with built-in sample storage mechanism according to claim 2, characterized in that: The second oblique support rod (12) is provided with a connecting pin (14), the connecting pin (14) passes through the first oblique support rod (11) and the second oblique support rod (12) at the same time, the end of the connecting pin (14) is bent and extended, and the first oblique support rod (11) is provided with a locking hole (13), and the end of the connecting pin (14) can be inserted into the locking hole (13).

4. The liquid nitrogen lifting pot with built-in sample storage mechanism according to claim 1, characterized in that: The positioning mechanism comprises a positioning rod (7) bent at 90°, one end of the positioning rod (7) being fixed to the support column (6), and the other end of the positioning rod (7) being bent upward to form a positioning hook.

5. The liquid nitrogen lifting pot with built-in sample storage mechanism according to claim 4, characterized in that: The protective cover (3) is provided with a through hole, the support column (6) passes through the through hole, the protective cover (3) is provided with a through slot (9) and an arc-shaped slot (8), the through slot (9) is connected to the through hole, the other end of the through slot (9) is connected to the arc-shaped slot (8), and the positioning rod (7) can pass through the through slot (9) and the arc-shaped slot (8).

6. The liquid nitrogen lifting pot with built-in sample storage mechanism according to claim 1, characterized in that: An end cover (2) is provided at the top end of the support column (6), and a handle (1) is provided on the end cover (2).