Mouse brain fixing instrument capable of being flexibly adjusted

Abstract

The utility model discloses a mouse brain fixing instrument capable of being flexibly adjusted, which comprises a base, and a mouse body fixing mechanism is arranged at the rear end of the base; a mouse brain fixing table is arranged in the middle of the base, two locking stand columns are arranged at one end of the mouse brain fixing table, adjustable supporting arms are arranged at the top ends of the two locking stand columns, the tail ends of the adjustable supporting arms are connected to mouse brain fixing pieces, four stand columns are arranged at the other end of the mouse brain fixing table, and ear rods are inserted into the middles of the two stand columns respectively. The position of the mouse brain fixing piece is finely adjusted front, back, left and right through the adjustable supporting arm, a micropore of the fixing piece is accurately aligned with a laboratory mouse skull windowing position, adjustment and use are flexible, the laboratory mouse is convenient to take and place, applicability is higher, and the brain imaging effect is effectively guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental mouse fixing equipment, in particular to a mouse brain fixing device which can be flexibly adjusted. Background Art

[0002] In the live mouse brain imaging experiment, whether the mouse's head is firmly fixed plays a key role in the success of the experiment. Before the experiment, a mouse brain fixator will be used to lock the experimental mouse. The mouse brain fixator commonly used in the laboratory is mainly composed of a base, a mouse body clamping mechanism and a mouse brain fixing mechanism. The body of the experimental mouse is locked on the clamping mechanism, and the microholes opened in the mouse skull are fixed to the mouse brain fixing mechanism by bolts, and finally the mouse brain is imaged and observed under a microscope. For example, a mouse brain fixator with application number CN202022672335.1 includes a base, a body fixer is provided on the base, a head fixer is provided on the side of the body fixer, a lifting mechanism is also provided on the base, a lifting platform is provided on the lifting mechanism, and the head fixator is fixed to the lifting platform; the above common types of experimental mouse brain fixators still have the following shortcomings in actual applications:

[0003] Due to differences in skull sizes among different experimental mice, the fixing angle of the fixation plate cannot be flexibly adjusted, resulting in a high failure rate in mouse brain imaging observations. At the same time, the bottom of the mouse brain is suspended in the air after fixation. Once the anesthetized experimental mouse wakes up and twists its head, it is easy for the fixation plate to fall off. Utility Model Content

[0004] The utility model aims to provide a flexibly adjustable rat brain fixation device to solve the problems of the existing experimental rat brain fixation device proposed in the above background technology that the device cannot be flexibly adjusted and has insufficient stability after fixation.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a flexibly adjustable mouse brain fixation instrument, comprising a base, a mouse body fixing mechanism for fixing experimental mice is fixedly arranged on one side of the top of the base, a mouse brain fixing platform is fixedly arranged in the middle of the top of the base, two locking columns are fixedly arranged on one side of the top of the mouse brain fixing platform, the tops of the two locking columns are rotatably provided with adjustable arms, one end of the two adjustable arms are provided with elliptical locking holes, an observation component for mouse brain fixation is installed between the two elliptical locking holes, the tops of the two adjustable arms are provided with first locking nails, the other side of the top of the mouse brain fixing platform is fixedly provided with a stimulation component for assisting mouse brain experiments, and the other side of the top of the base is fixedly provided with an adjustable brain support mechanism.

[0006] Preferably, the mouse body fixing mechanism includes a mouse body fixing platform and a mouse body fixing cover hinged on the mouse body fixing platform, a mouse body groove is opened in the middle of the top of the mouse body fixing platform, a hanging buckle is fixedly installed on the front of the mouse body fixing platform, a hanging buckle frame is fixedly arranged on the mouse body fixing cover, and the hanging buckle matches the hanging buckle frame.

[0007] Preferably, the observation assembly includes a fixing plate and locking bolts arranged at both ends of the fixing plate, the two locking bolts are respectively threadedly arranged on the inner sides of two elliptical lock holes, a reserved observation hole is opened in the middle of the fixing plate, and four upper mouse brain fixing microholes are opened on the periphery of the reserved observation hole.

[0008] Preferably, the fixing plate is configured as a rounded triangle.

[0009] Preferably, the stimulation component includes two stimulation columns and a needle slide hole opened in the middle of the stimulation columns, a stimulation needle is slidably arranged between the two needle slide holes, the top ends of the two stimulation columns are threadedly connected with a second locking pin, and the bottom end of the second locking pin extends into the interior of the needle slide hole.

[0010] Preferably, the adjustable brain support mechanism includes two U-shaped frames and fixed columns fixed on the U-shaped frames, a head support slide is slidably arranged between the two U-shaped frames, a mouse brain auxiliary fixing component is fixedly arranged at one end of the head support slide, and a locking limit component is fixedly arranged at the other end of the head support slide.

[0011] Preferably, the mouse brain auxiliary fixation component includes a lower support plate fixed to the bottom of the head support slide and lower mouse brain fixing microholes opened on the surface of the lower support plate, two directional sliding columns are fixedly arranged on the top of one end of the head support slide, a front tooth pressure plate is slidably arranged between the two directional sliding columns, a screw sleeve is fixedly arranged on the surface of the front tooth pressure plate, the inner side of the screw sleeve is threadedly connected with a locking screw, and the top end of the locking screw is rotatably arranged on the head support slide.

[0012] Preferably, the locking and limiting assembly includes an adjusting slide hole formed on the head support slide and a locking stud arranged inside the adjusting slide hole, and the locking stud is threadedly connected to the head support slide.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] The fixing plate of the experimental mouse brain fixation instrument is fixed on two supporting arms by bolts, and the position of the mouse brain fixing plate can be fine-tuned forward, backward, left and right by using the adjustable supporting arms according to the actual situation of the experimental mouse skull, so that the micro-holes of the fixing plate are accurately aligned with the window of the experimental mouse skull. The adjustment and use are flexible, the experimental mouse is convenient to take and put, the applicability is stronger, and the effect of mouse brain imaging is effectively guaranteed;

[0015] A brain support mechanism is added to the base, which can not only be adjusted forward and backward according to the fixed position of the mouse brain, but also can assist in fixing the mouse brain. The lower part of the mouse brain is fixed on the lower support plate to prevent the bottom of the mouse brain from falling empty. After fixation, it is stable and reliable, which can effectively prevent the problem of the mouse brain falling off during the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of the mouse brain fixation device of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the mouse brain fixation platform of the utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the adjustable brain support mechanism of the utility model;

[0019] Figure 4 This is a schematic diagram of the structure of the mouse body fixing mechanism of the utility model;

[0020] Figure 5 It is a top cross-sectional view of the adjustable swing arm of the utility model.

[0021] In the figure: 1. base; 2. mouse brain fixing platform; 3. adjustable brain support mechanism; 31. fixing column; 32. U-shaped frame; 33. head support slide; 34. adjustment slide hole; 35. locking stud; 36. incisor pressure plate; 37. directional slide column; 38. locking screw; 39. lower support plate; 310. lower mouse brain fixing microhole; 311. screw sleeve; 4. mouse body fixing mechanism; 41. mouse body fixing platform; 42. mouse body fixing cover; 43. hanging buckle; 44. hanging buckle frame; 45. mouse body groove; 5. locking column; 6. stimulation column; 7. adjustable support arm; 8. fixing plate; 9. locking bolt; 10. reserved observation hole; 11. upper mouse brain fixing microhole; 12. first locking nail; 13. second locking nail; 14. needle sliding hole; 15. stimulation needle; 16. elliptical locking hole. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0023] See also Figure 1-5The utility model provides a flexibly adjustable mouse brain fixation instrument, comprising a base 1, a mouse body fixing mechanism 4 for fixing experimental mice is fixedly arranged on one side of the top of the base 1, a mouse brain fixing platform 2 is fixedly arranged in the middle of the top of the base 1, two locking columns 5 are fixedly arranged on one side of the top of the mouse brain fixing platform 2, and adjustable arms 7 are rotatably arranged on the tops of the two locking columns 5, and an elliptical locking hole 16 is opened at one end of the two adjustable arms 7, and an observation component for fixing the mouse brain is installed between the two elliptical locking holes 16, and a first locking nail 12 is arranged on the tops of the two adjustable arms 7, a stimulation component for assisting mouse brain experiments is fixedly arranged on the other side of the top of the mouse brain fixing platform 2, and an adjustable brain support mechanism 3 is fixedly arranged on the other side of the top of the base 1.

[0024] See also Figure 1 and Figure 4 Further, the mouse body fixing mechanism 4 includes a mouse body fixing platform 41 and a mouse body fixing cover 42 hinged on the mouse body fixing platform 41, a mouse body groove 45 is opened in the middle of the top of the mouse body fixing platform 41, a hanging buckle 43 is fixedly installed on the front of the mouse body fixing platform 41, and a hanging buckle frame 44 is fixedly arranged on the mouse body fixing cover 42, and the hanging buckle 43 matches the hanging buckle frame 44;

[0025] When in use, open the mouse body fixing cover 42, place the anesthetized experimental mouse body on the mouse body groove 45 to expose the mouse head, cover the mouse body fixing cover 42, and press the hanging buckle 43 to hang the hanging buckle 43 on the hanging buckle frame 44. At this time, the experimental mouse is fixed.

[0026] See also Figure 1 and Figure 2 , further, the observation assembly includes a fixing plate 8 and locking bolts 9 arranged at both ends of the fixing plate 8, the two locking bolts 9 are respectively threadedly arranged on the inner sides of the two elliptical lock holes 16, a reserved observation hole 10 is opened in the middle of the fixing plate 8, four upper mouse brain fixing microholes 11 are opened on the periphery of the reserved observation hole 10, and the fixing plate 8 is set to a rounded triangle;

[0027] When in use, take out the fixing plate 8 and place it above the mouse brain so that the reserved observation hole 10 is directly opposite to the part of the mouse brain to be observed. Use small screws to pass through the upper mouse brain fixing microholes 11 in sequence to fix the fixing plate 8 on the opening of the mouse brain skull. Finally, rotate the adjustable support arm 7 to fix the fixing plate 8 on the adjustable support arm 7. The operation is simple.

[0028] See also Figure 1 and Figure 2 Further, the stimulation assembly includes two stimulation columns 6 and a needle sliding hole 14 opened in the middle of the stimulation column 6, a stimulation needle 15 is slidably arranged between the two needle sliding holes 14, and the top ends of the two stimulation columns 6 are both threadedly connected with a second locking nail 13, and the bottom end of the second locking nail 13 extends to the inside of the needle sliding hole 14;

[0029] During use, during the experiment, the experimenter can first loosen the second locking pin 13, push the stimulation needle 15, make the needle tip of the stimulation needle 15 close to the mouse brain, stimulate the mouse brain, and then turn the second locking pin 13 to lock the stimulation needle 15, so as to observe the neural state of the mouse brain when stimulated.

[0030] See also Figure 1 and Figure 3 Furthermore, the adjustable brain support mechanism 3 includes two U-shaped frames 32 and a fixed column 31 fixedly arranged on the U-shaped frames 32, a head support slide 33 is slidably arranged between the two U-shaped frames 32, one end of the head support slide 33 is fixedly provided with a mouse brain auxiliary fixing component, and the other end of the head support slide 33 is fixedly provided with a locking limit component, the mouse brain auxiliary fixing component includes a lower support plate 39 fixed to the bottom of the head support slide 33 and a lower mouse brain fixing microhole 310 opened on the surface of the lower support plate 39, and the head support slide 33 is fixedly provided with a mouse brain auxiliary fixing component. Two directional slides 37 are fixedly arranged on the top, and a front tooth pressure plate 36 is slidably arranged between the two directional slides 37. A screw sleeve 311 is fixedly arranged on the surface of the front tooth pressure plate 36. A locking screw 38 is threadedly connected to the inner side of the screw sleeve 311. The top end of the locking screw 38 is rotatably arranged on the head support slide 33. The locking limit assembly includes an adjustment slide hole 34 opened on the head support slide 33 and a locking stud 35 arranged on the inner side of the adjustment slide hole 34. The locking stud 35 is threadedly connected to the head support slide 33.

[0031] When in use, first loosen the locking stud 35, push the head support slide 33 forward until the mouse head can be placed on the lower support plate 39, and re-tighten the locking stud 35. Under the squeezing of the locking stud 35, the head support slide 33 is fixed and cannot slide. Use a small screw to pass through the lower mouse brain fixing microhole 310 to fix the microhole in the lower jaw of the mouse brain, and then turn the locking screw 38. The locking screw 38 drives the screw sleeve 311 to move, and then drives the incisor pressure plate 36 to slide on the directional slide column 37, clamping the upper teeth of the mouse and pressing the mouse's mouth and nose, so as to support and fix the bottom of the experimental mouse, prevent the experimental mouse head from being suspended in the air, improve the stability of the experiment, and prevent it from being detached, so as to increase the success rate of the experiment.

[0032] When the embodiment of the present application is used: open the mouse body fixing cover 42, place the anesthetized experimental mouse body on the mouse body groove 45, cover the mouse body fixing cover 42, detent the hanging buckle 43, and hang the hanging buckle 43 on the hanging buckle frame 44. At this time, the mouse body is fixed. According to the position of the mouse head, first loosen the locking stud 35, push the head support slide 33 forward until the mouse head can be placed on the lower support plate 39, and re-tighten the locking stud 35. Under the pressure of the locking stud 35, the head support slide 33 is fixed and cannot slide. Use a small screw to pass through the lower mouse brain fixing microhole 310 to fix the microhole in the lower jaw of the mouse brain, and then turn the locking stud 35. The screw 38 and the locking screw 38 drive the screw sleeve 311 to move, thereby driving the incisor pressure plate 36 to slide on the directional slide column 37 to press the mouse's mouth and nose. Finally, the fixing plate 8 is taken out and placed above the mouse's brain so that the reserved observation hole 10 is facing the part of the mouse's brain to be observed. Small screws are used to pass through the upper mouse brain fixing microholes 11 in sequence to fix the fixing plate 8 on the opening part of the mouse's skull. Then, the two adjustable support arms 7 are rotated in sequence, and the fixing plate 8 is placed on the two adjustable support arms 7. The fixing plate 8 is locked and fixed by the locking bolt 9. Finally, the two first locking nails 12 are tightened. At this time, the mouse's brain is fixed and experimental observation under a microscope can be carried out.

[0033] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A flexibly adjustable rat brain fixation device, comprising a base (1), characterized in that: A mouse body fixing mechanism (4) for fixing an experimental mouse is fixedly arranged on one side of the top of the base (1); a mouse brain fixing platform (2) is fixedly arranged in the middle of the top of the base (1); two locking columns (5) are fixedly arranged on one side of the top of the mouse brain fixing platform (2); adjustable arms (7) are rotatably arranged on the tops of the two locking columns (5); an elliptical locking hole (16) is provided at one end of the two adjustable arms (7); an observation component for fixing a mouse brain is installed between the two elliptical locking holes (16); a first locking pin (12) is provided at the top of the two adjustable arms (7); a stimulation component for assisting a mouse brain experiment is fixedly arranged on the other side of the top of the mouse brain fixing platform (2); and an adjustable brain support mechanism (3) is fixedly arranged on the other side of the top of the base (1).

2. The flexibly adjustable rat brain fixation device according to claim 1, characterized in that: The mouse body fixing mechanism (4) comprises a mouse body fixing platform (41) and a mouse body fixing cover (42) hinged on the mouse body fixing platform (41); a mouse body groove (45) is provided in the middle of the top of the mouse body fixing platform (41); a hanging buckle (43) is fixedly installed on the front of the mouse body fixing platform (41); a hanging buckle frame (44) is fixedly arranged on the mouse body fixing cover (42); and the hanging buckle (43) matches the hanging buckle frame (44).

3. The flexibly adjustable rat brain fixation device according to claim 1, characterized in that: The observation assembly comprises a fixing plate (8) and locking bolts (9) arranged at both ends of the fixing plate (8), wherein the two locking bolts (9) are respectively threadedly arranged on the inner sides of two elliptical lock holes (16), and a reserved observation hole (10) is opened in the middle of the fixing plate (8), and four upper mouse brain fixing microholes (11) are opened on the periphery of the reserved observation hole (10).

4. The flexibly adjustable rat brain fixation device according to claim 3, characterized in that: The fixing plate (8) is configured as a rounded triangle.

5. The flexibly adjustable rat brain fixation device according to claim 1, characterized in that: The stimulation component comprises two stimulation columns (6) and a needle slide hole (14) provided in the middle of the stimulation columns (6); a stimulation needle (15) is slidably arranged between the two needle slide holes (14); the top ends of the two stimulation columns (6) are both threadedly connected with a second locking pin (13); the bottom end of the second locking pin (13) extends into the interior of the needle slide hole (14).

6. The flexibly adjustable rat brain fixation device according to claim 1, characterized in that: The adjustable brain support mechanism (3) comprises two U-shaped frames (32) and a fixing column (31) fixedly arranged on the U-shaped frames (32); a head support slide (33) is slidably arranged between the two U-shaped frames (32); a mouse brain auxiliary fixing component is fixedly arranged at one end of the head support slide (33); and a locking limiter component is fixedly arranged at the other end of the head support slide (33).

7. The flexibly adjustable rat brain fixation device according to claim 6, characterized in that: The mouse brain auxiliary fixation component comprises a lower support plate (39) fixed to the bottom of the head support slide (33) and a lower mouse brain fixing microhole (310) opened on the surface of the lower support plate (39); two directional slide columns (37) are fixedly arranged on the top of one end of the head support slide (33); a front tooth pressure plate (36) is slidably arranged between the two directional slide columns (37); a screw sleeve (311) is fixedly arranged on the surface of the front tooth pressure plate (36); the inner side of the screw sleeve (311) is threadedly connected to a locking screw (38); the top end of the locking screw (38) is rotatably arranged on the head support slide (33).

8. The flexibly adjustable rat brain fixation device according to claim 6, characterized in that: The locking and limiting assembly comprises an adjusting slide hole (34) formed on the head support slide (33) and a locking stud (35) arranged inside the adjusting slide hole (34); the locking stud (35) is threadedly connected to the head support slide (33).

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