Polymerase chain reaction cabinet

Abstract

The utility model discloses a polymerase chain reaction cabinet which comprises a cabinet body and a temperature control mechanism, guide rails are fixedly connected to the inner walls of the left side and the right side of the cabinet body, a reaction drawer is slidably connected between the two guide rails, and sliding rails in bilateral symmetry are fixedly connected to the bottom wall of the reaction drawer; the temperature control mechanism comprises a first fixing base, a heating piece, a reaction assembly, a fixing assembly and a semiconductor chilling plate, the first fixing base is arranged on the front side of the middle of the bottom wall of the reaction drawer, the heating piece is fixedly connected to the upper surface of the first fixing base, and the fixing assembly is arranged on the rear side of the middle of the bottom wall of the reaction drawer; the upper surface of the fixing assembly is fixedly connected with a semiconductor refrigeration sheet, the refrigeration surface of the semiconductor refrigeration sheet faces upwards, a reaction assembly is slidably connected between the two sliding rails, and the lower surface of the reaction assembly is in contact with the upper surface of the heating sheet. The chain reaction rate is accelerated.

Description

Technical Field

[0001] The utility model relates to the technical field of polymerase chain reaction, in particular to a polymerase chain reaction cabinet. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be seen as a special DNA replication outside the body. The biggest feature of PCR is that it can greatly increase trace amounts of DNA. Therefore, whether it is ancient organisms in fossils, remains of historical figures, or hair, skin or blood left by murderers in homicides decades ago, as long as a tiny bit of DNA can be isolated, it can be amplified by PCR for comparison.

[0003] PCR uses the fact that DNA denatures into a single strand at 95°C in vitro. At low temperatures (usually around 60°C), primers and single strands combine according to the principle of base complementary pairing. The temperature is then adjusted to the optimal reaction temperature of DNA polymerase (around 72°C). DNA polymerase synthesizes complementary chains along the direction from phosphate to pentose (5'-3'). The PCR instrument manufactured based on polymerase is actually a temperature control device that can well control the denaturation temperature, annealing temperature, and extension temperature. Existing PCR instruments usually use air cooling or natural cooling to cool down, and one cycle takes a long time. For this reason, we propose a polymerase chain reaction cabinet. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the existing defects and provide a polymerase chain reaction cabinet, which can achieve rapid and uniform heating or cooling by replacing the reaction chamber, accelerate the chain reaction rate, and effectively solve the problems in the background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a polymerase chain reaction cabinet, comprising a cabinet body and a temperature control mechanism;

[0006] Cabinet: The inner walls on both sides are fixedly connected with guide rails, a reaction drawer is slidably connected between the two guide rails, and the bottom wall of the reaction drawer is fixedly connected with left-right symmetrical slide rails;

[0007] Temperature control mechanism: it includes a fixing seat 1, a heating plate, a reaction component, a fixing component and a semiconductor cooling plate. A fixing seat 1 is provided on the front side of the middle of the bottom wall of the reaction drawer, and the upper surface of the fixing seat 1 is fixedly connected with the heating plate. A fixing component is provided on the rear side of the middle of the bottom wall of the reaction drawer, and the upper surface of the fixing component is fixedly connected with the semiconductor cooling plate, and the cooling surface of the semiconductor cooling plate faces upward. The reaction component is slidably connected between the two slide rails, and the lower surface of the reaction component contacts the upper surface of the heating plate.

[0008] It also includes a single-chip microcomputer, which is arranged on the upper surface of the cabinet. The input end of the single-chip microcomputer is electrically connected to an external power supply, and the input ends of the heating plate and the semiconductor cooling plate are electrically connected to the output end of the single-chip microcomputer. Rapid and uniform heating or cooling can be achieved by replacing the reaction chamber, thereby accelerating the chain reaction rate.

[0009] Furthermore, the reaction assembly includes a slide, a temperature sensor, a temperature control chamber, a sample rack and limit columns. The slide is slidably connected between two slide rails, the lower surface of the slide is in contact with the upper surface of the heating plate, the upper surface of the slide is provided with a temperature control chamber, and the four corners of the upper end of the temperature control chamber are symmetrically provided with limit columns. The sample rack is inserted between the four limit columns, and temperature sensors are provided on the left and right sides of the temperature control chamber. The temperature sensors are bidirectionally electrically connected to the single-chip microcomputer to achieve precise internal temperature control.

[0010] Furthermore, the temperature control mechanism also includes a mounting seat, an electric push rod and connecting bolts. Mounting seats are provided on the left and right sides of the front end of the bottom wall of the reaction drawer. The upper ends of the mounting seats are fixedly connected to the electric push rods. The left and right sides of the rear side of the upper surface of the slide are threadedly connected with connecting bolts. The lower ends of the connecting bolts are respectively inserted into the inside of the telescopic ends of the adjacent electric push rods. The input ends of the electric push rods are electrically connected to the output ends of the single-chip microcomputer to push the slide to slide.

[0011] Furthermore, the temperature control mechanism also includes a water outlet pipe. The lower end of the slide is provided with a water outlet pipe, the water outlet pipe is connected to the interior of the temperature control chamber, the front end of the water outlet pipe is threadedly connected with a plugging cover, the front end of the reaction drawer is provided with a avoidance port, the external thread of the avoidance port is connected with an end cover, and the plugging cover is located inside the end cover to facilitate water drainage.

[0012] Furthermore, the fixing assembly includes a second fixing seat, a heat sink and glass fiber insulation cotton. A second fixing seat is provided on the rear side of the middle part of the bottom wall of the reaction drawer. The interior of the second fixing seat is fixedly connected with the heat sink. The inner wall of the second fixing seat is provided with glass fiber insulation cotton. The glass fiber insulation cotton is located between the second fixing seat and the heat sink. A semiconductor refrigeration plate is fixedly connected to the upper surface of the heat sink to dissipate heat for the semiconductor refrigeration plate.

[0013] Furthermore, a rubber pad is provided at the front end of the cabinet, the front side of the reaction drawer contacts the front side of the rubber pad, and the upper end of the cabinet is rotatably connected to a limit seat symmetrically distributed on the left and right through a pin shaft, and the edges of the limit seat respectively contact the front side of the reaction drawer to fix the reaction drawer.

[0014] Furthermore, a display screen is provided on the upper surface of the cabinet, and an input end of the display screen is electrically connected to an output end of the single-chip microcomputer, and the current water temperature is displayed in graphics and text.

[0015] Compared with the prior art, the beneficial effects of the utility model are: the polymerase chain reaction cabinet has the following advantages:

[0016] The sample is loaded into the sample rack, and then the reaction drawer is pushed into the cabinet. The limit seat is rotated, and the edges of the limit seat are respectively in contact with the front side of the reaction drawer to fix the reaction drawer. The heating plate is turned on by the single-chip microcomputer, and the heating plate heats the water inside the temperature control chamber. The temperature sensor detects the water temperature inside the temperature control chamber in real time and feeds back to the single-chip microcomputer. The single-chip microcomputer displays the current water temperature through the display screen. The temperature sensors on both sides respectively detect the adjacent water temperatures to make the detection result more accurate. After the water temperature reaches the denaturation temperature for a period of time, the single-chip microcomputer controls the telescopic end of the electric push rod to be pushed out, and the slide slides to the right between the two slide rails. The lower surface of the slide contacts the cooling surface of the semiconductor refrigeration plate, the semiconductor refrigeration plate is turned on, and the water temperature drops rapidly. After reaching the renaturation temperature, the semiconductor refrigeration plate is turned off. After waiting for a set period of time, the telescopic end of the electric push rod retracts, the slide returns to its original position, and the heating plate is heated to make the water temperature quickly return to the extension temperature. After repeatedly passing through the denaturation temperature, renaturation temperature, and extension temperature, the DNA of the sample increases significantly. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the internal structure of the cabinet of the utility model;

[0019] Figure 3 It is a schematic cross-sectional structural diagram of the reaction drawer of the utility model.

[0020] In the figure: 1 cabinet, 2 temperature control mechanism, 21 water outlet pipe, 22 fixing seat 1, 23 mounting seat, 24 heating plate, 25 electric push rod, 26 connecting bolt, 27 reaction component, 271 slide, 272 temperature sensor, 273 temperature control chamber, 274 sample rack, 275 limit column, 28 fixing component, 281 fixing seat 2, 282 heat sink, 283 glass fiber insulation cotton, 29 semiconductor refrigeration plate, 3 reaction drawer, 4 end cover, 5 rubber cushion, 6 limit seat, 7 display screen, 8 single chip computer, 9 plug cover, 10 slide rail, 11 guide rail. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1-3 ,This embodiment provides a technical solution: a polymerase chain reaction cabinet, including a cabinet body 1 and a temperature control mechanism 2;

[0023] Cabinet 1: The inner walls on both sides thereof are fixedly connected with guide rails 11, a reaction drawer 3 is slidably connected between the two guide rails 11, the bottom wall of the reaction drawer 3 is fixedly connected with left-right symmetrical slide rails 10, a rubber cushion 5 is arranged at the front end of the cabinet 1, the front side of the reaction drawer 3 contacts the front side of the rubber cushion 5, the upper end of the cabinet 1 is rotatably connected with left-right symmetrically distributed limit seats 6 through a pin shaft, the edges of the limit seats 6 contact the front side of the reaction drawer 3 respectively, a display screen 7 is arranged on the upper surface of the cabinet 1, the input end of the display screen 7 is electrically connected to the output end of the single-chip computer 8, the sample is loaded into the sample rack 274, and then the reaction drawer 3 is pushed into the cabinet 1, the limit seat 6 is rotated, the edges of the limit seat 6 contact the front side of the reaction drawer 3 respectively, and the reaction drawer 3 is fixed;

[0024] Temperature control mechanism 2: It includes a fixed seat 22, a heating plate 24, a reaction component 27, a fixed component 28 and a semiconductor cooling plate 29. A fixed seat 22 is provided on the front side of the middle of the bottom wall of the reaction drawer 3, and the upper surface of the fixed seat 22 is fixedly connected with the heating plate 24. A fixed component 28 is provided on the rear side of the middle of the bottom wall of the reaction drawer 3. A semiconductor cooling plate 29 is fixedly connected to the upper surface of the fixed component 28. The cooling surface of the semiconductor cooling plate 29 faces upward. A reaction component 27 is slidably connected between the two slide rails 10. The lower surface of the reaction component 27 contacts the upper surface of the heating plate 24. The reaction component 27 includes a slide 271, a temperature sensor 272, a temperature control chamber 273, a sample rack 274 and a limit column 275. The slide 271 is slidably connected to the two slide rails 10. The lower surface of the slide 271 contacts the upper surface of the heating plate 24, and the upper surface of the slide 271 is provided with a temperature control chamber 273. The four corners of the upper end of the temperature control chamber 273 are symmetrically provided with limit columns 275. A sample rack 274 is inserted between the four limit columns 275. The left and right sides of the temperature control chamber 273 are provided with temperature sensors 272. The temperature sensors 272 are bidirectionally electrically connected to the single-chip computer 8. The temperature control mechanism 2 also includes a mounting seat 23, an electric push rod 25 and a connecting bolt 26. The left and right sides of the front end of the bottom wall of the reaction drawer 3 are provided with mounting seats 23. The upper ends of the mounting seats 23 are fixedly connected with electric push rods 25. The left and right sides of the rear side of the upper surface of the slide 271 are threadedly connected with connecting bolts 26. The lower ends of the connecting bolts 26 They are respectively plugged into the inside of the telescopic ends of the adjacent electric push rods 25, and the input ends of the electric push rods 25 are electrically connected to the output ends of the single-chip computer 8. The temperature control mechanism 2 also includes a water outlet pipe 21. The lower end of the slide 271 is provided with a water outlet pipe 21, and the water outlet pipe 21 is connected to the inside of the temperature control chamber 273. The front end of the water outlet pipe 21 is threadedly connected with a plugging cover 9. The front end of the reaction drawer 3 is provided with an avoidance port, and the external thread of the avoidance port is connected with an end cover 4. The plugging cover 9 is located inside the end cover 4. The fixing component 28 includes a fixing seat 281, a heat sink 282 and glass fiber insulation cotton 283. The rear side of the middle part of the bottom wall of the reaction drawer 3 is provided with a fixing seat 281, and the interior of the fixing seat 281 is fixedly connected with a heat sink 282, and the bottom end of the heat sink 282 penetrates the reaction drawer 3. The bottom wall is convenient for heat extraction. The inner wall of the second fixing seat 281 is provided with glass fiber insulation cotton 283. The glass fiber insulation cotton 283 is located between the second fixing seat 281 and the heat sink 282. The upper surface of the heat sink 282 is fixedly connected with a semiconductor refrigeration sheet 29. The heat generating end of the semiconductor refrigeration sheet 29 is fixedly connected to the upper surface of the heat sink 282. The generated heat is extracted in time through the heat sink 282. The heating sheet 24 is turned on by the single-chip microcomputer 8. The heating sheet 24 heats the water inside the temperature control chamber 273. The temperature sensor 272 detects the water temperature inside the temperature control chamber 273 in real time and feeds it back to the single-chip microcomputer 8. The single-chip microcomputer 8 displays the current water temperature through the display screen 7. The temperature sensors 272 on both sides respectively detect the adjacent water temperatures to make the detection result more accurate.After the water temperature reaches the denaturation temperature for a period of time, the single chip computer 8 controls the telescopic end of the electric push rod 25 to be pushed out, and the slide 271 slides to the right between the two slide rails 10. The lower surface of the slide 271 contacts the cooling surface of the semiconductor cooling sheet 29, and the semiconductor cooling sheet 29 is turned on. The water temperature drops rapidly. After reaching the renaturation temperature, the semiconductor cooling sheet 29 is turned off. After waiting for a set period of time, the telescopic end of the electric push rod 25 is retracted, the slide 271 returns to its original position, and the heating sheet 24 is heated to make the water temperature quickly return to the extension temperature;

[0025] It also includes a single-chip microcomputer 8, which is arranged on the upper surface of the cabinet 1. The input end of the single-chip microcomputer 8 is electrically connected to an external power supply, and the input ends of the heating plate 24 and the semiconductor cooling plate 29 are both electrically connected to the output end of the single-chip microcomputer 8.

[0026] The working principle of a polymerase chain reaction cabinet provided by the utility model is as follows: the testing personnel first set the water temperature inside the temperature control chamber 273 to reach the denaturation temperature, annealing temperature, and the residence time after the extension temperature through the single-chip microcomputer 8, add water to the inside of the temperature control chamber 273 until the water level exceeds the height of the sample inside the sample tube, load the sample into the sample rack 274, and then push the reaction drawer 3 into the cabinet 1, rotate the limit seat 6, the edges of the limit seat 6 are respectively in contact with the front side of the reaction drawer 3, and the reaction drawer 3 is fixed, and the heating plate 24 is turned on by the single-chip microcomputer 8, and the heating plate 24 heats the water inside the temperature control chamber 273, and the temperature sensor 272 detects the water temperature inside the temperature control chamber 273 in real time and feeds back to the single-chip microcomputer 8, and the single-chip microcomputer 8 displays the current water temperature through the display screen 7, and the two sides The temperature sensor 272 detects the adjacent water temperatures respectively to make the detection result more accurate. After the water temperature reaches the denaturation temperature for a period of time, the single-chip microcomputer 8 controls the telescopic end of the electric push rod 25 to be pushed out, and the slide 271 slides to the right between the two slide rails 10. The lower surface of the slide 271 contacts the cooling surface of the semiconductor refrigeration plate 29, and the semiconductor refrigeration plate 29 is turned on. The water temperature drops rapidly, and the semiconductor refrigeration plate 29 is turned off after reaching the renaturation temperature. After waiting for a set period of time, the telescopic end of the electric push rod 25 retracts, the slide 271 returns to its original position, and the heating plate 24 is heated to make the water temperature quickly return to the extension temperature. After repeatedly passing through the denaturation temperature, renaturation temperature, and extension temperature, the DNA of the sample increases significantly. After use, the slide 271 is returned to its original position, and the end cover 4 and the plug cover 9 are unscrewed to drain the water.

[0027] It is worth noting that the single chip microcomputer 8 disclosed in the above embodiments can use STM32F103C8T6, the semiconductor refrigeration plate 29 can use TEC1-12707 semiconductor refrigeration plate, the temperature sensor 272 can use QAE26.93 water temperature sensor, and the electric push rod 25 can use SD50 electric push rod. The single chip microcomputer 8 controls the heating plate 24, the semiconductor refrigeration plate 29, the temperature sensor 272 and the electric push rod 25 using methods commonly used in the prior art.

[0028] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A polymerase chain reaction cabinet, characterized in that: It comprises a cabinet (1) and a temperature control mechanism (2); The cabinet (1) has guide rails (11) fixedly connected to the inner walls on both sides thereof, a reaction drawer (3) is slidably connected between the two guide rails (11), and a left-right symmetrical slide rail (10) is fixedly connected to the bottom wall of the reaction drawer (3); The temperature control mechanism (2) comprises a fixing seat (22), a heating plate (24), a reaction component (27), a fixing component (28) and a semiconductor cooling plate (29); a fixing seat (22) is provided on the front side of the middle of the bottom wall of the reaction drawer (3); the upper surface of the fixing seat (22) is fixedly connected to the heating plate (24); a fixing component (28) is provided on the rear side of the middle of the bottom wall of the reaction drawer (3); the upper surface of the fixing component (28) is fixedly connected to the semiconductor cooling plate (29); the cooling surface of the semiconductor cooling plate (29) faces upward; a reaction component (27) is slidably connected between the two slide rails (10); the lower surface of the reaction component (27) contacts the upper surface of the heating plate (24); It also includes a single-chip microcomputer (8), which is arranged on the upper surface of the cabinet (1), the input end of the single-chip microcomputer (8) is electrically connected to an external power supply, and the input ends of the heating plate (24) and the semiconductor cooling plate (29) are both electrically connected to the output end of the single-chip microcomputer (8).

2. A polymerase chain reaction cabinet according to claim 1, characterized in that: The reaction assembly (27) comprises a slide (271), a temperature sensor (272), a temperature control chamber (273), a sample rack (274) and a limiting column (275). The slide (271) is slidably connected between two slide rails (10). The lower surface of the slide (271) contacts the upper surface of the heating plate (24). The upper surface of the slide (271) is provided with a temperature control chamber (273). The limiting columns (275) are symmetrically arranged at the four corners of the upper end of the temperature control chamber (273). The sample rack (274) is inserted between the four limiting columns (275). The temperature sensors (272) are arranged on the left and right sides of the temperature control chamber (273). The temperature sensors (272) are bidirectionally electrically connected to the single-chip computer (8).

3. A polymerase chain reaction cabinet according to claim 2, characterized in that: The temperature control mechanism (2) also includes a mounting seat (23), an electric push rod (25) and a connecting bolt (26). The left and right sides of the front end of the bottom wall of the reaction drawer (3) are provided with mounting seats (23). The upper ends of the mounting seats (23) are fixedly connected to the electric push rods (25). The left and right sides of the rear side of the upper surface of the slide table (271) are threadedly connected to the connecting bolts (26). The lower ends of the connecting bolts (26) are respectively inserted into the inside of the telescopic ends of the adjacent electric push rods (25). The input ends of the electric push rods (25) are electrically connected to the output ends of the single-chip computer (8).

4. A polymerase chain reaction cabinet according to claim 2, characterized in that: The temperature control mechanism (2) further comprises a water outlet pipe (21), the lower end of the slide (271) is provided with the water outlet pipe (21), the water outlet pipe (21) is communicated with the interior of the temperature control chamber (273), the front end of the water outlet pipe (21) is threadedly connected to a plugging cover (9), the front end of the reaction drawer (3) is provided with an escape port, the outer portion of the escape port is threadedly connected to an end cover (4), and the plugging cover (9) is located inside the end cover (4).

5. A polymerase chain reaction cabinet according to claim 1, characterized in that: The fixing assembly (28) comprises a second fixing seat (281), a heat sink (282) and glass fiber heat insulation cotton (283); a second fixing seat (281) is provided on the rear side of the middle part of the bottom wall of the reaction drawer (3); the interior of the second fixing seat (281) is fixedly connected to the heat sink (282); the inner wall of the second fixing seat (281) is provided with glass fiber heat insulation cotton (283); the glass fiber heat insulation cotton (283) is located between the second fixing seat (281) and the heat sink (282); the upper surface of the heat sink (282) is fixedly connected to a semiconductor cooling plate (29).

6. A polymerase chain reaction cabinet according to claim 1, characterized in that: The front end of the cabinet (1) is provided with a rubber cushion (5), the front side of the reaction drawer (3) contacts the front side of the rubber cushion (5), and the upper end of the cabinet (1) is rotatably connected to a limit seat (6) symmetrically distributed on the left and right through a pin shaft, and the edges of the limit seat (6) respectively contact the front side of the reaction drawer (3).

7. A polymerase chain reaction cabinet according to claim 1, characterized in that: A display screen (7) is provided on the upper surface of the cabinet (1), and an input end of the display screen (7) is electrically connected to an output end of a single-chip computer (8).

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