PCR adapter for space transcriptome chip hybridization

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) adapter for space transcriptome chip hybridization, which comprises an adapter plate for space transcriptome chip hybridization of a 96-hole PCR instrument chip, the adapter plate is provided with two surfaces, one surface is provided with two groups of metal convex blocks, and the other surface is provided with 96 groups of conical rods. According to the invention, one surface of the adapter plate is provided with two groups of metal protruding blocks, two groups of space chips can be placed on the metal protruding blocks at the same time, the convenience is greatly improved when the space chips are placed or taken, the adapter plate is suitable for chips of various models, the working efficiency is effectively improved, and the cost is reduced. Moreover, due to the design that the surfaces of the metal protruding blocks are flat, abrasion to the chip can be reduced, the phenomenon of sliding off is avoided, and due to the fact that the adaptation plate is made of aluminum alloy materials, the heat conduction performance is good, reaction temperature control is more accurate, and cost is low.

Description

Technical Field

[0001] The utility model belongs to the technical field of spatial transcriptome chip adapters, and in particular relates to a PCR adapter for spatial transcriptome chip hybridization. Background Art

[0002] Spatial transcriptomics is an emerging and timely method that combines tissue sections with transcriptome sequencing to obtain spatial information and transcript information. It is a technology that analyzes and describes the expression profile of specific cell types in a spatial dimension to understand the expression differences between organs, tissues and pathological states. Compared with single-cell transcriptomics, it provides spatial location information, providing a more precise research direction for disease research, and is of great significance for in-depth understanding of disease pathogenic mechanisms and targeted treatment.

[0003] At present, the Chinese patent with the publication (announcement) number of CN216712064U discloses a spatial transcriptome chip, including: a substrate, a substrate forms a plurality of rectangular micropore areas, and a plurality of sub-rectangular micropore areas are formed inside the rectangular micropore area, and a plurality of micropore structures are evenly distributed in each sub-rectangular micropore area, and the micropore structure is used to place the coded microspheres. A spatial transcriptome chip provided by the utility model is constructed with a plurality of rectangular micropore areas on a substrate, and a plurality of sub-rectangular micropore areas are also formed in each rectangular micropore area, and a micropore structure is evenly distributed in each sub-rectangular micropore area, and the spatial transcriptome resolution is improved by the micropore structure, and a highly sensitive detection is achieved to meet the needs of scientists for subcellular structure analysis; and a glass substrate can be used, and a micropore structure can be made by etching technology, and its manufacturing process is simple, reducing the cost of consumables.

[0004] However, during the above-mentioned spatial transcriptome experiment, the chip (made of glass) needs to be placed in a PCR instrument for reaction. Therefore, an adapter that can fix the chip and has good thermal conductivity is needed as a carrier for the chip so that the chip can be used for experimental reaction in the PCR instrument. The existing adapter is provided in the 10X test kit and is only used for 10X chips. It is expensive and a universal chip adapter is needed to meet daily experimental needs. Utility Model Content

[0005] In order to solve the above technical problems, the purpose of the utility model is to provide a PCR adapter for spatial transcriptome chip hybridization, so as to solve the technical defect mentioned in the above technology that an adapter that can fix the chip and has good thermal conductivity is needed to serve as a carrier for the chip so that the chip can perform experimental reactions in a PCR instrument.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a PCR adapter for spatial transcriptome chip hybridization, comprising an adapter plate for 96-well PCR instrument chip spatial transcriptome chip hybridization, wherein the adapter plate is provided with two sides, one side has two groups of metal protrusions, and the other side has 96 groups of conical rods.

[0007] As a further solution of the present invention, the adapter plate is made of aluminum alloy.

[0008] As a further preferred embodiment of the present invention, the tapered rods are arranged in 8 rows horizontally and 12 columns vertically, for a total of 96 groups.

[0009] As a preferred solution of the utility model, a protrusion block is respectively provided at one end of the bottom of the conical rod, and the protrusion block is in the shape of a triangular conical metal protrusion.

[0010] As a further solution of the utility model, the protruding block is a triangular conical metal protrusion, which is consistent with the shape of the reaction tank set in the 96-well PCR instrument.

[0011] As a further solution of the utility model, a placement surface is provided at the inner center of the upper surface of the metal protruding block, and the placement surface is consistent with the size of the space chip.

[0012] Compared with the prior art, the PCR adapter for spatial transcriptome chip hybridization provided by the utility model has the following beneficial effects:

[0013] 1. Two sets of metal protrusions are provided on one side of the adapter plate, and two sets of space chips can be placed on the metal protrusions at the same time, which greatly improves the convenience when placing or taking. It can be applied to chips of various models, effectively improving work efficiency. The flat surface design of the metal protrusions can reduce the wear of the chip and prevent it from slipping. Since the adapter plate is made of aluminum alloy, it has good thermal conductivity, more accurate reaction temperature control, and low cost.

[0014] 2. A group is provided through the conical rod, which is consistent with the shape of the reaction tank of the 96-well PCR, so that the staff can place the adapter plate on the 96-well PCR instrument for use, thereby improving convenience. The raised block at the bottom of the conical rod is in the shape of a triangular conical metal raised block, which is convenient for placing the adapter plate when not in use. The raised block at the bottom of the conical rod leaves space at the bottom of the adapter plate, which is more convenient for taking and cleaning.

[0015] 3. The placement surface provided inside the upper surface of the metal protrusion can be suitable for chips of various sizes and specifications, reducing the problem of chip mismatch and affecting work efficiency, so as to effectively complete the chip adaptation work, and has good thermal conductivity and is easy to fix. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solution of the embodiment of the utility model, the drawings required for use in the embodiment will be briefly introduced below. Obviously, the drawings described below are only examples of the embodiment of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 It is a three-dimensional schematic diagram of the overall structure of an embodiment of the utility model;

[0018] Figure 2 This is a side physical diagram of the overall structure of an embodiment of the utility model;

[0019] Figure 3 This is a front view of the overall structure of an embodiment of the utility model;

[0020] Figure 4 This is a real picture of the back side of the overall structure of an embodiment of the utility model.

[0021] Reference numerals:

[0022] 1. Adapter board;

[0023] 2. tapered rod; 21. raised block;

[0024] 3. Metal protrusion; 31. Placement surface. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the embodiment of the utility model more clear, the following is a further detailed description of the embodiment of the utility model in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0026] In the description of the embodiments of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the embodiments of the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limitations on the embodiments of the present invention.

[0027] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly stipulated and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, an integral connection, or a detachable connection; it can be the internal connection of two components; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0028] See attached Figure 1 To Attachment Figure 4 As shown, a PCR adapter for spatial transcriptome chip hybridization includes an adapter plate 1 for 96-well PCR instrument chip spatial transcriptome chip hybridization, the adapter plate 1 has two sides, one side has two groups of metal protrusions 3, and the other side has 96 groups of conical rods 2, and the adapter plate 1 is made of aluminum alloy.

[0029] Among them, two groups of metal protrusions 3 are provided on one side of the adapter plate 1, and two groups of space chips can be placed on the metal protrusions 3 at the same time, which greatly improves the convenience when placing or taking. It can be applicable to chips of various models and effectively improves work efficiency. The smooth surface design of the metal protrusions 3 can reduce the wear of the chip and will not slip. Since the adapter plate 1 is made of aluminum alloy, it has good thermal conductivity, more accurate reaction temperature control, and low cost.

[0030] See attached Figure 1 -Attached Figure 2 As shown, the conical rods 2 are arranged in 8 horizontal rows and 12 vertical columns, with a total of 96 groups. A protrusion 21 is provided at one end of the bottom of the conical rods 2. The protrusion 21 is in the shape of a triangular conical metal protrusion, which is consistent with the shape of the reaction tank set in the 96-well PCR instrument.

[0031] Among them, 96 groups are provided through the conical rod 2, which is consistent with the shape of the reaction tank of the 96-well PCR, so that the staff can place the adapter plate 1 on the 96-well PCR instrument for use, thereby improving convenience. The protruding block 21 is provided at the bottom of the conical rod 2. The protruding block 21 is in the shape of a triangular conical metal protrusion, which is convenient for placement when the adapter plate 1 is not in use. The protruding block 21 at the bottom of the conical rod 2 leaves space at the bottom of the adapter plate 1, which is more convenient for taking and cleaning.

[0032] See attached Figure 4 As shown, a placement surface 31 is provided at the inner center of the upper surface of the metal protruding block 3, and the placement surface 31 is consistent with the size of the space chip.

[0033] Among them, the placement surface 31 provided inside the upper surface of the metal protrusion block 3 can be suitable for chips of various sizes and specifications, reducing chip mismatch and affecting work efficiency, so that the chip adaptation work can be effectively completed, and the thermal conductivity is good and it is easy to fix.

[0034] Working principle: When in use, place the adapter plate 1 on a 96-well PCR instrument, preheat it to the reaction temperature in advance, then place the chip on the placement surface 31 area on the metal protrusion 3 provided on the adapter plate 1, start the reaction program, and after the reaction is completed, remove the chip first, then remove the adapter plate 1, and use 75% alcohol to clean it for the next use.

[0035] The above shows and describes the basic principles of the present invention. The above are only preferred embodiments of the present invention and are not intended to limit the present invention. The above embodiments and descriptions in the specification are only intended to illustrate the principles of the present invention. Without departing from the scope of the present invention, any modifications, equivalent substitutions and improvements made within the spirit and scope of the present invention should be included in the protection scope of the present invention.

Claims

1. A PCR adapter for spatial transcriptome chip hybridization, characterized in that: The invention comprises an adapter plate (1) for 96-well PCR chip spatial transcriptome chip hybridization, wherein the adapter plate (1) is provided with two surfaces, one surface is provided with two groups of metal protruding blocks (3), and the other surface is provided with 96 groups of conical rods (2), a placement surface (31) is provided at the inner center of the upper surface of the metal protruding block (3), and the placement surface (31) is consistent with the size of the spatial chip.

2. A PCR adapter for spatial transcriptome chip hybridization according to claim 1, characterized in that: The adapter plate (1) is made of aluminum alloy.

3. A PCR adapter for spatial transcriptome chip hybridization according to claim 1, characterized in that: The tapered rods (2) are arranged in 8 rows horizontally and 12 columns vertically, with a total of 96 groups.

4. A PCR adapter for spatial transcriptome chip hybridization according to claim 3, characterized in that: A protruding block (21) is provided at one end of the bottom of the conical rod (2), and the protruding block (21) is in the shape of a triangular conical metal protrusion.

5. A PCR adapter for spatial transcriptome chip hybridization according to claim 4, characterized in that: The protruding block (21) is a triangular conical metal protrusion, which is consistent with the shape of the reaction tank set in the 96-well PCR instrument.

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