Respiration and metabolism oxygen consumption measuring device

Abstract

The utility model discloses a respiration and metabolism oxygen consumption measuring device which comprises an experiment cabin, an air inlet assembly and a differential pressure sensor, the experiment module is internally provided with an adsorption material for absorbing water and carbon dioxide; the air inlet assembly comprises a resistance valve, one end of the air inlet assembly is communicated with the experiment module through a second pipeline, the other end of the air inlet assembly is communicated with the atmosphere or a normal pressure air storage bag, a positive pressure interface of a differential pressure sensor is communicated with the experiment module through a first pipeline, and a negative pressure interface of the differential pressure sensor is communicated with the atmosphere; the differential pressure sensor is used for collecting air pressure changes in the experiment module and obtaining respiration data of a detected object according to the air pressure changes, and the respiration data comprises respiration frequency and / or lung ventilation capacity. The respiratory data such as the respiratory rate and the lung gas energy change of a detected object can be obtained without an oxygen sensor, the device is used for measuring the metabolic oxygen consumption, the service life of equipment is prolonged, and the cost is reduced.

Description

Technical Field

[0001] The present application relates to a respiratory measurement device, and in particular to a respiratory and metabolic oxygen consumption measurement device. Background Art

[0002] Hypoxia experiment is one of the basic teaching experiments commonly carried out in courses such as pathophysiology and human function. By observing the physiological and behavioral changes of rats, mice and other animals under hypoxia, we can understand the effects of different types of hypoxia on the body, in order to further provide a basis for the treatment and research of hypoxia-related diseases. Traditional hypoxia experiments usually use wide-mouth bottle manual observation method, tracheal intubation method, xiphoid process recording method, etc. The above methods are either unable to quantitatively monitor the respiratory movements of rats and mice, or need to be performed under anesthesia and trauma, and are unable to quantitatively monitor metabolic oxygen consumption in real time, so there are many deficiencies in methodology.

[0003] In recent years, quantitative monitoring devices for hypoxia experiments in rats and mice in a conscious state have been commercialized. These quantitative monitoring devices for hypoxia experiments mainly use oxygen sensors for detection, but oxygen sensors are expensive and have a short service life (usually less than two years). Therefore, due to factors such as the price of equipment and the price and life of oxygen sensors, the cost of using such devices to carry out teaching experiments is too high and it is still difficult to popularize them in teaching experiments. Therefore, how to increase the service life of the quantitative monitoring device for hypoxia experiments and reduce its price has become a technical problem that needs to be solved for the popularization of the device. Utility Model Content

[0004] In view of the above problems, the present application provides a respiratory and metabolic oxygen consumption measuring device to solve the technical problems of the short service life and high price of the above-mentioned existing devices.

[0005] To achieve the above objectives, the present application provides a respiratory and metabolic oxygen consumption measuring device, comprising:

[0006] The experimental cabin is a closed cabin for accommodating the test object and having built-in adsorption materials for absorbing water and carbon dioxide generated by the test object during the measurement process;

[0007] An air intake assembly, comprising a resistance valve, one end of which is connected to the experimental chamber through a second pipe, and the other end of which is connected to the atmosphere or to a normal pressure air storage bag, wherein oxygen or other gas is stored in the normal pressure air storage bag, and the normal pressure air storage bag maintains an internal pressure equal to that of the atmosphere; the resistance valve is arranged in series on the air intake path of the air intake assembly, and is used to increase the flow resistance of the gas flowing into and out of the experimental chamber;

[0008] The differential pressure sensor comprises a positive pressure interface and a negative pressure interface, wherein the positive pressure interface is connected to the experimental chamber through a first pipe, and the negative pressure interface is connected to the atmosphere; the differential pressure sensor is used to collect the air pressure changes in the experimental chamber.

[0009] Furthermore, the air intake assembly also includes an air extraction device and a selection valve;

[0010] The selection valve and the resistance valve are arranged in series, and the air extraction device is connected to the selection valve. The selection valve is used to select the air extraction device to be connected to the experimental chamber or to be connected to the normal pressure air storage bag or the atmosphere, so as to extract a predetermined amount of gas in the normal pressure air storage bag or a predetermined amount of atmosphere into the experimental chamber.

[0011] Furthermore, the selection valve is a three-way stopcock valve or an electromagnetic three-way valve, and the selection valve includes at least three connection ends, the first connection end is connected to the experimental cabin through the second pipe, the second connection end is connected to the air extraction device, and the third connection end is connected to the atmosphere or connected to the normal pressure air storage bag.

[0012] Furthermore, the air extraction device is a piston syringe or a syringe pump.

[0013] Furthermore, the resistance valve is an adjustable resistance valve assembly, including a resistance valve body with fixed resistance and a rotary regulating valve with adjustable conduction resistance connected in series with the resistance valve body; the rotary regulating valve is a throttle valve or a pressure regulating valve.

[0014] Furthermore, the experimental chamber comprises a bottle-shaped chamber body having an opening and a sealing plug adapted to the opening; the first pipe and the second pipe extend into the chamber body through the sealing plug;

[0015] The adsorption material is soda lime particles, which are spread on the bottom of the cabin to form an adsorption layer or a mixture of soda lime particles and color-changing silica gel particles; one end of the second pipe connected to the sealing plug is flush with the bottom surface of the sealing plug;

[0016] The first pipe extends into one end of the cabin and is inserted into the shallow area of ​​the adsorption layer.

[0017] Furthermore, the portion of the first pipe extending into the cabin is eccentrically arranged in the cabin.

[0018] Furthermore, it also includes a data collector and a display, wherein the data collector is connected to the differential pressure sensor and the display, and the data collector is used to receive the air pressure changes and send them to the display for display.

[0019] Different from the prior art, the above technical solution does not use expensive and easily worn oxygen sensors, but uses a combination of a differential pressure sensor and a resistance valve to detect the relative pressure change in the experimental chamber and the external atmospheric pressure. The pressure change can directly reflect the breathing frequency of the test subject. And according to Boyle's law and the ideal gas state equation pV=nRT, the breathing data such as the breathing frequency and lung gas energy change of the test subject can be calculated.

[0020] In some embodiments, since the water and carbon dioxide in the experimental chamber are absorbed by the adsorption material, the above-mentioned change in air pressure difference is positively correlated with the change in oxygen in the experimental chamber; when the experimental chamber is sealed, the oxygen consumption of the detection object can be monitored in real time through the above-mentioned change in air pressure difference.

[0021] The above-mentioned records related to the content of the utility model are only an overview of the technical solution of the present application. In order to enable ordinary technicians in the field to more clearly understand the technical solution of the present application, and then implement it according to the text of the specification and the contents recorded in the drawings, and to make the above-mentioned purpose and other purposes, features and advantages of the present application easier to understand, the following is an explanation in combination with the specific implementation method and drawings of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The drawings are only used to illustrate the principles, implementation methods, applications, features and effects of the specific embodiments of the present invention and other related contents, and shall not be considered as limitations of the present application.

[0023] In the drawings of the specification:

[0024] Figure 1 It is a structural schematic diagram of the respiratory and metabolic oxygen consumption measuring device described in a specific embodiment;

[0025] Figure 2a This is a schematic diagram of the structure of the experimental chamber and the first pipeline and the second pipeline described in the specific implementation method;

[0026] Figure 2b It is a structural schematic diagram of the experimental cabin described in another specific implementation mode;

[0027] Figure 3 It is a structural schematic diagram of the adjustable resistance valve described in the specific implementation method;

[0028] Figure 4 The breathing data of the detection object collected by the differential pressure sensor described in the specific implementation manner;

[0029] The reference numerals in the above drawings are described as follows:

[0030] 1. Experimental cabin; 11. Cabin body; 12. Sealing plug; 13. Second pipeline; 14. First pipeline; 15. Gas injection channel; 16. Valve;

[0031] 2. Differential pressure sensor; 21. Positive pressure interface; 22. Negative pressure interface;

[0032] 3. Air intake assembly; 31. Selector valve; 32. Resistance valve; 33. Air extraction device; 321. Resistance valve body; 322. Rotary regulating valve;

[0033] 4. Normal pressure air storage bag;

[0034] 5. Data collector;

[0035] 6. Display;

[0036] 7. Adsorption materials; DETAILED DESCRIPTION

[0037] In order to explain in detail the possible application scenarios, technical principles, specific schemes that can be implemented, and the purposes and effects that can be achieved, the following is a detailed description of the specific embodiments listed in conjunction with the accompanying drawings. The embodiments described herein are only used to more clearly illustrate the technical solutions of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0038] Reference to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or association with other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the various technical features mentioned in the embodiments can be combined in any way to form a corresponding implementable technical solution.

[0039] Unless otherwise defined, the technical terms used in this document have the same meanings as those generally understood by those skilled in the art to which this application belongs; the use of relevant terms in this document is only for describing specific embodiments and is not intended to limit this application.

[0040] In the description of this application, the term "and / or" is an expression used to describe the logical relationship between objects, indicating that three relationships may exist, for example, A and / or B, which means: A exists, B exists, and A and B exist at the same time. In addition, the character " / " in this article generally indicates that the objects before and after are in an "or" logical relationship.

[0041] In the present application, terms such as “first” and “second” are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship of quantity, priority or sequence between these entities or operations.

[0042] Without further limitations, in this application, the words "include", "comprises", "has" or other similar open-ended expressions used in sentences are intended to cover non-exclusive inclusion. These expressions do not exclude the presence of additional elements in the process, method or product including the elements, so that the process, method or product including a series of elements may include not only those limited elements, but also other elements not explicitly listed, or also include elements inherent to such process, method or product.

[0043] Similar to the understanding in the Examination Guidelines, in this application, expressions such as "greater than", "less than", "exceed" and the like are understood to exclude the number itself; expressions such as "above", "below", "within" and the like are understood to include the number itself. In addition, in the description of the embodiments of this application, "multiple" means more than two (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups", "multiple times", etc., unless otherwise clearly and specifically limited.

[0044] In the description of the embodiments of the present application, space-related expressions used, such as "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "vertical", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicate the orientation or position relationship based on the orientation or position relationship shown in the specific embodiments or drawings, and are only for the convenience of describing the specific embodiments of the present application or facilitating the reader's understanding, and do not indicate or imply that the referred device or component must have a specific position, a specific orientation, or be constructed or operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.

[0045] Unless otherwise expressly specified or limited, in the description of the embodiments of the present application, the terms such as "install", "connect", "connect", "fix", "set", etc. used should be understood in a broad sense. For example, the "connection" can be a fixed connection, a detachable connection, or an integrated setting; it can be a mechanical connection, an electrical connection, or a communication connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. For technicians in the technical field to which the present application belongs, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0046] See also Figure 1 This embodiment provides a respiratory and metabolic oxygen consumption measuring device. The respiratory and metabolic oxygen consumption measuring device can be used to measure the oxygen consumption, respiratory frequency and other respiratory data of various test objects such as mice and rats, and to perform hypoxia experiments on the test objects.

[0047] like Figure 1 As shown, in this embodiment, the respiratory and metabolic oxygen consumption measuring device includes: an experimental cabin 1, a differential pressure sensor 2 and an air intake assembly 3. The air extraction device 33 can be a manual piston syringe or an electric injection pump. The injection pump is connected to a power supply and a control switch, and the injection pump can be powered on by the control switch to pump the gas in the atmosphere or the normal pressure gas storage bag 4 into the experimental cabin 1.

[0048] Among them, the experimental cabin 1 is a closed cabin body 11 for accommodating the detection object, and an adsorbent material 7 is built in to absorb the water and carbon dioxide generated by the detection object during the measurement process. The experimental cabin 1 has an opening, and the detection object can be put in and taken out from the opening. The size of the experimental cabin 1 can be determined according to the size and quantity of the detection object. In some embodiments, the experimental cabin 1 includes a bottle-shaped cabin body 11 with an opening and a sealing plug 12 adapted to the opening; the adsorbent material 7 is soda lime particles, and of course, the adsorbent material 7 is not limited to soda lime particles. Specifically, in one embodiment, the experimental cabin 1 is a 250mL washing bottle as the cabin body 11, and the washing bottle is equipped with a sealing plug 12, and the soda lime particles are loaded into the bottom of the bottle to the 50mL scale position of the washing bottle to absorb the carbon dioxide and water produced by breathing. In some embodiments, the adsorbent material 7 can also be a mixture of soda lime particles and color-changing silica gel particles, wherein the color-changing silica gel particles can be used to absorb water vapor in the experimental cabin 1, and the soda lime particles focus on absorbing carbon dioxide in the experimental cabin 1. That is, in some embodiments, the adsorption material 7 may be only soda lime particles, while in other embodiments, in order to have a good effect of absorbing water and carbon dioxide, the adsorption material 7 may also be a mixture of soda lime particles and color-changing silica gel particles.

[0049] The air intake assembly 3 includes a resistance valve 32. One end of the air intake assembly 3 is connected to the experimental cabin 1 through the second pipe 13, and the other end of the air intake assembly 3 is connected to the atmosphere or to the normal pressure air storage bag 4. The normal pressure air storage bag 4 stores oxygen or other gases, and the normal pressure air storage bag 4 maintains an internal pressure equal to that of the atmosphere. The resistance valve 32 is arranged in series on the air intake path of the air intake assembly 3, and the resistance valve 32 is used to increase the flow resistance of the gas exchange in the experimental cabin 1. The normal pressure air storage bag 4 can be made by yourself using a fresh-keeping bag or a thin-walled rubber bladder.

[0050] The air intake assembly 3 is used to connect the experimental chamber 1 with the atmosphere or the normal pressure air storage bag 4, so that when the air intake assembly 3 is opened, the gas in the atmosphere or the normal pressure air storage bag 4 can enter the experimental chamber 1 (when the air intake assembly 3 is closed, the experimental chamber 1 forms a sealed space, and the gas cannot enter or exit the experimental chamber 1). It should be noted that the air intake assembly 3 does not limit the flow direction of the gas to only enter the experimental chamber 1 from the outside. When the air intake assembly 3 is opened, when the test subject breathes in the experimental chamber 1, the gas will enter and be discharged from the experimental chamber 1 through the air intake assembly 3 along with the breathing frequency.

[0051] The differential pressure sensor 2 includes a positive pressure interface 21 and a negative pressure interface 22, the positive pressure interface 21 is connected to the experimental cabin 1 through a first pipe 14, and the negative pressure interface 22 is connected to the atmosphere; the differential pressure sensor 2 is used to collect the air pressure changes in the experimental cabin 1, and obtain the breathing data of the detection object according to the air pressure changes, and the breathing data includes breathing frequency and / or lung ventilation volume.

[0052] The negative pressure interface 22 of the differential pressure sensor 2 is connected to the outside atmosphere, and the pressure difference between the gas pressure inside the experimental cabin 1 and the atmospheric pressure is calculated based on the atmospheric pressure outside the experimental cabin 1. The differential pressure sensor 2 is connected to the data collector 5, and the data collector 5 is connected to the display 6, so the data collected by the differential pressure sensor 2 can be displayed on the display 6 through the data collector 5.

[0053] In this embodiment, the end of the first pipe 14 connected to the experimental chamber 1 is completely closed by the differential pressure sensor 2, and the resistance valve 32 connected to the second pipe 13 produces a certain resistance to the gas flow, thereby also having a certain sealing effect on the experimental chamber 1.

[0054] When the experimental chamber 1 is completely sealed, the temperature T in the chamber remains stable, the sum V of the extracorporeal space volume and the lung volume of the test object (such as a mouse or rat) is a constant, and the total mass n of the gas inside and outside the body of the test object in the experimental chamber 1 is a constant, it can be known from Boyle's law and the ideal gas state equation pV=nRT that the air pressure measured by the differential pressure sensor 2 at the end of inspiration and the end of exhalation are equal (air pressure baseline value). During the inhalation process, the chest and abdomen of the test object expand outward, the extracorporeal space volume of the test object decreases and the air pressure increases, the lung volume of the test object increases and the intrapulmonary pressure decreases, and the gas enters the lungs driven by the pressure difference. Due to the limited expansion of the thorax and the lungs, as the gas enters the lungs, the intrapulmonary pressure gradually changes from decreasing to increasing, while the extracorporeal space pressure changes from increasing to decreasing, until the extracorporeal space pressure is equal to the intrapulmonary pressure again, and the inhalation ends. Specifically, during the inhalation phase, the air pressure in the space outside the test subject's body first increases from the baseline air pressure level, increases to a certain maximum value, and then turns to decrease until it drops to the baseline air pressure level. This is the positive phase wave of the inhalation phase; the exhalation phase is the opposite negative phase wave. The air pressure signal in the experimental cabin 1 is collected by the differential pressure sensor 2, and the respiratory rate data of the test subject can be obtained in real time. After the positive or negative phase wave of the air pressure fluctuation is integrated over the duration t of inspiration or exhalation, the area value can be used to represent the change in the lung ventilation volume of the test subject. However, it should be noted that when the volume difference between the test subjects is large, the area value can only be used for comparison before and after the intervention of the same individual.

[0055] Since the detection object breathes and consumes oxygen in the experimental cabin 1, the carbon dioxide and water produced by breathing are absorbed by soda lime, and the amount of gas substance n in the cabin will continue to decrease. Even if the breathing frequency and depth of the detection object do not change, the waveform of the positive and negative phase waves of breathing collected by the differential pressure sensor 2 will also change continuously, and cannot be used to evaluate the change of pulmonary ventilation. Therefore, in the present embodiment, a resistance valve 32 (preferably an adjustable resistance valve assembly) with a pipeline resistance much greater than the airway resistance of mice is designed to be connected to the atmosphere or the normal pressure airbag, and the pressure difference inside and outside the cabin caused by oxygen consumption can drive the external air or the gas in the normal pressure airbag to continuously enter the experimental cabin 1 through the resistance valve, maintaining the stability of the amount of gas substance n in the cabin. When the resistance of the resistance valve 32 is large enough, it has only a weak shunting effect on the pressure fluctuation caused by the detection object's breathing, and the breathing curve collected by the differential pressure sensor 2 can be used to analyze the change of the mouse pulmonary ventilation. In this embodiment, by increasing the flow resistance of gas exchange in the experimental chamber 1 through the resistance valve 32, the air intake component 3 can divert the air pressure fluctuation in the experimental chamber 1 caused by the breathing of the test subject, so that the air pressure change collected by the differential pressure sensor 2 can map the change of the lung ventilation volume of the test subject.

[0056] In the above embodiment, the respiratory and metabolic oxygen consumption measuring device does not use expensive and easily worn oxygen sensors, but uses a combination of a differential pressure sensor 2 and a resistance valve 32 to detect the relative pressure difference change between the experimental chamber 1 and the external atmospheric pressure, which can intuitively reflect the respiratory frequency of the test object. And according to Boyle's law and the ideal gas state equation pV=nRT, the respiratory data such as the respiratory frequency and lung gas energy change of the test object are calculated. Thereby, the service life of the respiratory and metabolic oxygen consumption measuring device is greatly improved, and its production and later maintenance costs are reduced.

[0057] like Figure 1 As shown, in one embodiment, the air intake assembly 3 also includes an air extraction device 33 and a selection valve 31. The air extraction device 33 can be a manual piston syringe. The selection valve 31 is used to control whether the experimental cabin 1 is connected to the air extraction device 33 through the second pipeline 13 or to the atmosphere or the normal pressure air storage bag 4. In some embodiments, the selection valve 31 is a three-way valve or an electromagnetic three-way valve, and the selection valve 31 includes at least three connecting ends, the first connecting end is connected to the experimental cabin 1 through the second pipeline 13, the second connecting end is connected to the air extraction device 33, and the third connecting end is connected to the atmosphere or connected to the normal pressure air storage bag 4.

[0058] The selection valve 31 and the resistance valve 32 are arranged in series, and the air extraction device 33 is connected to the selection valve 31. The selection valve 31 is used to select the air extraction device 33 to be connected to the experimental chamber 1 or to be connected to the normal pressure air storage bag 4 or the atmosphere, so as to extract a predetermined amount of gas in the normal pressure air storage bag 4 or a predetermined amount of atmosphere into the experimental chamber 1. The selection valve 31 is also used to close the air intake assembly 3 after the predetermined amount of atmosphere or the gas is extracted, collect the time required for the set amount of air pressure decay in the experimental chamber 1 or the air pressure decay amount within the set time through the differential pressure sensor 2, and calculate the metabolic oxygen consumption of the detection object.

[0059] When the air intake assembly 3 further includes an air extraction device 33 and a selection valve 31, the respiratory and metabolic oxygen consumption measuring device can be used to monitor oxygen consumption in real time. A certain amount (e.g., 1 mL) of atmosphere or oxygen can be pumped into the experimental chamber 1 through the air intake assembly 3, and then the experimental chamber 1 is sealed to monitor oxygen consumption.

[0060] For example, in one embodiment, the stopcock tee on the infusion tube is selected as the above-mentioned selector valve 31, and a 2mL syringe is selected as the above-mentioned air extraction device 33, and then the oxygen consumption is measured by gas injection pressure measurement. Use a 2mL disposable syringe to accurately extract a certain volume of air or oxygen according to the experimental needs and connect it with the infusion stopcock tee. When measuring oxygen consumption, the stopcock tee is rotated so that the experimental cabin 1 is only connected to the disposable syringe, and the gas in the disposable syringe is immediately injected into the experimental cabin 1, and the air pressure in the cabin increases, and the respiratory curve baseline recorded by the differential pressure sensor 2 rises to a certain level immediately. The mouse breathes in the experimental cabin 1, and oxygen is continuously consumed. The exhaled carbon dioxide and water are absorbed by soda lime, and the air pressure in the cabin is gradually reduced. When the air pressure returns to the atmospheric pressure level, the stopcock tee is rotated again so that the experimental cabin 1 remains connected to the stopcock tee, and the respiratory curve is continuously recorded. Use a respiratory and metabolic oxygen consumption measuring device to measure the time required for the baseline of the respiratory curve to recover from the highest value to the atmospheric pressure level after gas injection. Divide the injected gas volume by the air pressure recovery time to quantify the mouse oxygen consumption rate, expressed in mL·g-1·h-1. The changes in the mouse oxygen consumption rate can also be qualitatively analyzed by observing the changes in the baseline slope of the respiratory curve.

[0061] like Figure 2a As shown, in one embodiment, the experimental chamber 1 comprises a bottle-shaped chamber body 11 with an opening and a sealing plug 12 matched with the opening; the first pipe 14 and the second pipe 13 extend into the chamber body 11 through the sealing plug 12; the adsorption material 7 is soda lime particles, and the adsorption material 7 is spread on the bottom of the chamber body 11 to form an adsorption layer. One end of the second pipe 13 connected to the sealing plug 12 is flush with the bottom surface of the sealing plug 12; one end of the first pipe 14 extends into the chamber body 11 and is inserted into the shallow area of ​​the adsorption layer.

[0062] In this embodiment, the first pipe 14 is inserted into the superficial area of ​​the adsorption layer, and the second pipe 13 is flush with the bottom surface of the sealing plug 12, so that the detection object (such as a mouse) in the cabin can be prevented from approaching the pipe openings of the first pipe 14 and the second pipe 13, thereby avoiding the detection data deviation caused by the movement of the detection object, and ensuring the accuracy and data reliability of the respiratory and metabolic oxygen consumption measuring device. In addition, in one embodiment, in order to prevent the detection object from moving around the first pipe 14 in the experimental cabin 1, the part of the first pipe 14 extending into the cabin body 11 is eccentrically arranged in the cabin body 11. Since the movement of the detection object will change its cardiopulmonary exercise and change the temperature in the experimental cabin 1, which will affect the measurement results, in this embodiment, the first pipe 14 is eccentrically arranged in the cabin body 11, which can prevent the detection object from moving around it, and ensure the accuracy and data reliability of the respiratory and metabolic oxygen consumption measuring device.

[0063] like Figure 2bAs shown, in one embodiment, the experimental cabin 1 is further provided with an injection channel 15 and a valve 16, wherein the valve 16 is connected in series to the injection channel 15. When the valve 16 is opened, carbon monoxide, nitric oxide and other gases required for the breathing experiment can be injected into the experimental cabin 1 through the injection channel 15. Preferably, the diameter of the injection channel 15 and / or the chamber volume of the valve 16 should be set relatively small (for example, the inner diameter of the injection channel 15 is less than 1 mm), so that the injection channel 15 and the valve 16 have good air tightness, and at the same time, the switching moment between the opening and closing states of the valve 16 will not have a significant impact on the internal air pressure of the experimental cabin 1.

[0064] In this embodiment, when the experimental chamber 1 includes a bottle body and a sealing plug 12, the gas injection channel 15 is provided on the sealing plug 12. When an experimental project needs to inject gas into the experimental chamber, such as studying the effects of carbon monoxide, nitric oxide, etc. on breathing, the valve 16 can be opened to inject experimental gas into the experimental chamber 1 through the gas injection channel 15, and then the valve 16 can be closed.

[0065] like Figure 3 As shown, in one embodiment, the resistance valve 32 is an adjustable resistance valve assembly, including a resistance valve body 321 with fixed resistance and a rotary regulating valve 322 with adjustable conduction resistance connected in series with the resistance valve body 321; the rotary regulating valve 322 can be a throttle valve or a pressure regulating valve. By rotating the knob on the throttle valve or the pressure regulating valve, the conduction area inside the throttle valve or the pressure regulating valve can be adjusted, thereby adjusting the conduction resistance of the throttle valve or the pressure regulating valve. Preferably, the rotary regulating valve 322 is a micro throttle valve or a micro pressure regulating valve.

[0066] In this embodiment, the resistance valve 32 is an adjustable resistance valve assembly, so the resistance of the air intake assembly 3 can be adjusted according to the individual size or number of the detection objects, so that the respiratory and metabolic oxygen consumption measurement device can be applicable to a variety of different types of detection objects and improve its flexibility of use.

[0067] like Figure 4 As shown in the figure, the breathing data of the test object collected by the differential pressure sensor 2 in the above embodiment. Part A is the waveform data of the oxygen consumption of the test object repeatedly measured 10 times in the awake state and the oxygen consumption of the test object repeatedly measured 10 times in the anesthetized state. Part B is the breathing frequency data of the test object mapped by the pressure change in the experimental cabin 1 collected by the differential pressure sensor 2.

[0068] Finally, it should be noted that although the above embodiments have been described in the specification and drawings of this application, this does not limit the scope of patent protection of this application. All technical solutions generated by replacing or modifying equivalent structures or equivalent processes based on the essential concept of this application using the contents recorded in the specification and drawings of this application, as well as directly or indirectly implementing the technical solutions of the above embodiments in other related technical fields, are included in the scope of patent protection of this application.

Claims

1. A respiratory and metabolic oxygen consumption measuring device, characterized in that: include: The experimental chamber is a closed chamber for accommodating the test object and having built-in adsorption materials for absorbing water and carbon dioxide generated by the test object during the measurement process; An air intake assembly, comprising a resistance valve, one end of which is connected to the experimental chamber through a second pipe, and the other end of which is connected to the atmosphere or to a normal pressure gas storage bag, wherein oxygen or other gases are stored in the normal pressure gas storage bag; the resistance valve is arranged in series on the air intake path of the air intake assembly, and is used to increase the flow resistance of the gas flowing into and out of the experimental chamber; The differential pressure sensor comprises a positive pressure interface and a negative pressure interface, wherein the positive pressure interface is connected to the experimental chamber through a first pipe, and the negative pressure interface is connected to the atmosphere; the differential pressure sensor is used to collect the air pressure changes in the experimental chamber.

2. The respiratory and metabolic oxygen consumption measuring device according to claim 1, characterized in that: The air intake assembly also includes an air extraction device and a gate valve; The selection valve and the resistance valve are arranged in series, and the air extraction device is connected to the selection valve. The selection valve is used to select the air extraction device to be connected to the experimental chamber or to be connected to the normal pressure air storage bag or the atmosphere, so as to extract a predetermined amount of gas in the normal pressure air storage bag or a predetermined amount of atmosphere into the experimental chamber.

3. The respiratory and metabolic oxygen consumption measuring device according to claim 2, characterized in that: The selection valve is a three-way plug valve or an electromagnetic three-way valve, and the selection valve includes at least three connection ends, the first connection end is connected to the experimental cabin through the second pipeline, the second connection end is connected to the air extraction device, and the third connection end is connected to the atmosphere or connected to the normal pressure air storage bag.

4. The respiratory and metabolic oxygen consumption measuring device according to claim 2, characterized in that: The air extraction device is a piston-type syringe or a syringe pump.

5. The respiratory and metabolic oxygen consumption measuring device according to claim 1, characterized in that: The resistance valve comprises a resistance valve body with fixed resistance and a rotary regulating valve with adjustable conduction resistance connected in series with the resistance valve body; the rotary regulating valve is a throttle valve or a pressure regulating valve.

6. The respiratory and metabolic oxygen consumption measuring device according to claim 1, characterized in that: The experimental chamber comprises a bottle-shaped chamber body with an opening and a sealing plug adapted to the opening; the first pipe and the second pipe extend into the chamber body through the sealing plug; The adsorption material is soda lime particles, which are spread on the bottom of the cabin to form an adsorption layer or a mixture of soda lime particles and color-changing silica gel particles; one end of the second pipe connected to the sealing plug is flush with the bottom surface of the sealing plug; The first pipe extends into one end of the cabin and is inserted into the shallow area of ​​the adsorption layer.

7. The respiratory and metabolic oxygen consumption measuring device according to claim 6, characterized in that: The portion of the first pipe extending into the cabin is eccentrically arranged in the cabin.

8. The respiratory and metabolic oxygen consumption measuring device according to claim 1, characterized in that: It also includes a data collector and a display. The data collector is connected to the differential pressure sensor and the display. The data collector is used to receive the air pressure change and send it to the display for display.

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