Auxiliary sieve plate imbedding device

Abstract

The utility model discloses a sieve plate auxiliary imbedding device, the sieve plate auxiliary imbedding device comprises a sieve plate mold and a bearing piece, the sieve plate mold and the bearing piece are arranged to be plates with the same size and shape, and the sieve plate mold is horizontally installed on the bearing piece. A plurality of uniformly distributed first holes are formed in the sieve plate mold, and the aperture of each first hole is equal to the diameter of the sieve plate. According to the sieve plate auxiliary placement device, the sieve plate can be directly adsorbed by the affinity chromatography column, the connecting rod of the affinity chromatography column extends into the affinity chromatography column and is connected with the negative pressure pump, and the sieve plate is adsorbed in the bottom of the affinity chromatography column, so that a user does not need to manually place the sieve plate, and the sieve plate is prevented from shifting or turning over; and the speed of filling the sieve plate into the affinity chromatography column is accelerated, and the operation process is simple, convenient and practical.

Description

Technical Field

[0001] The utility model relates to the technical field of affinity chromatography, in particular to a sieve plate auxiliary inserting device. Background Art

[0002] Some biological molecules, such as antigens and antibodies, recombinant proteins and some metal ions, have specific affinity, so they can bind tightly to each other under certain conditions and dissociate from each other when the conditions are changed. Affinity chromatography is a chromatography technology designed to utilize the specific affinity between biological molecules. The basic principle of this technology is to extract the target protein by introducing a specific ligand and utilizing the affinity between the ligand and the target protein.

[0003] The basic operation of affinity chromatography requires the use of affinity chromatography columns, fillers and sieve plates, among which the sieve plate needs to be placed in the affinity chromatography column to fix the fillers in the affinity chromatography column and control the flow rate of the solution flowing through the affinity chromatography column. The sieve plate is sintered from special raw materials and has excellent hydrophilicity, so the user needs to keep it dry when storing it to avoid the sieve plate from contacting the liquid and becoming ineffective. In the prior art, the user must manually place the sieve plate from the upper port of the affinity chromatography column to the bottom of the affinity chromatography column. This operation is not only cumbersome, but once the user operates improperly, the sieve plate may tilt or flip in the affinity chromatography column during the placement process, thereby affecting the effect of the affinity chromatography. Utility Model Content

[0004] One advantage of the utility model is that it provides a sieve plate auxiliary insertion device, through which the sieve plate on the sieve plate auxiliary insertion device can be adsorbed by the affinity chromatography column. The user stretches the plunger of the affinity chromatography column to insert the sieve plate into the lumen of the affinity chromatography column, without manually inserting the sieve plate from the tube mouth of the affinity chromatography column into the lumen, thereby avoiding the sieve plate from flipping or shifting during the process of inserting the sieve plate into the affinity chromatography column.

[0005] One advantage of the utility model is that it provides a sieve plate auxiliary insertion device, in which the sieve plates are evenly and dispersedly placed on the sieve plate mold of the sieve plate auxiliary insertion device for use. During operation, the user can directly insert the affinity chromatography column into the sieve plate auxiliary insertion device and adsorb the sieve plate, which is simple to operate.

[0006] One advantage of the utility model is that it provides a sieve plate auxiliary insertion device, which is provided with a positioning piece, and the affinity chromatography column can be stably and quickly inserted into the sieve plate auxiliary insertion device through the positioning piece, thereby accelerating the insertion speed of the sieve plate.

[0007] One advantage of the utility model is that it provides a sieve plate auxiliary insertion device, and by arranging a placement piece and a positioning piece to be magnetically attracted to each other, a plurality of sieve plate auxiliary insertion devices can be stacked and placed for use.

[0008] In order to achieve at least one of the above advantages of the present invention, the present invention provides a sieve plate auxiliary placement device, the sieve plate auxiliary placement device comprising:

[0009] A sieve plate mold, wherein the sieve plate mold forms a plurality of evenly distributed first holes;

[0010] A supporting member defines an upper surface and a lower surface, the sieve plate mold is tightly mounted on the upper surface, and the first hole and the upper surface form a placement cavity.

[0011] According to an embodiment of the utility model, the sieve plate auxiliary insertion device also includes a positioning member, which is implemented as a plate with a certain thickness and forms a plurality of evenly distributed second holes with the same aperture as the first holes of the sieve plate mold.

[0012] According to an embodiment of the utility model, the sieve plate auxiliary insertion device also includes a placement piece, and the placement piece is connected to the lower surface of the supporting member.

[0013] According to an embodiment of the present invention, the positioning member is tightly mounted on a side of the screen plate mold opposite to the supporting member, and the second holes correspond to the first holes one by one.

[0014] According to an embodiment of the present invention, the placement cavity is adapted to the outer diameter of the affinity chromatography column.

[0015] According to an embodiment of the present invention, the storage element is implemented as a frame with a certain height.

[0016] According to an embodiment of the present invention, the supporting member is implemented to have a grid or mesh, and a channel for air circulation is formed between the upper surface and the lower surface.

[0017] According to an embodiment of the present invention, the positioning member is implemented as a magnetically attractive material, the object placement member is implemented as a magnetically attractive material, and the object placement member and the positioning member are magnetically connected.

[0018] According to an embodiment of the present invention, the sieve plate is horizontally placed in the placement cavity.

[0019] According to an embodiment of the present invention, the affinity chromatography column is vertically inserted into the placement cavity. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1A stereoscopic view of the screen plate auxiliary insertion device of the utility model is shown.

[0021] Figure 2 A separation diagram of the positioning member of the sieve plate auxiliary insertion device of the utility model is shown.

[0022] Figure 3 An exploded view of the sieve plate auxiliary insertion device of the utility model is shown.

[0023] Figure 4 A stereoscopic view of another embodiment of the screen plate auxiliary insertion device of the utility model is shown.

[0024] Figure 5 An exploded view of another embodiment of the sieve plate auxiliary insertion device of the utility model is shown.

[0025] Figure 6 The figure shows the insertion diagram of the affinity chromatography column of the sieve plate auxiliary insertion device of the utility model.

[0026] Figure 7 An enlarged view of the affinity chromatography column of the sieve plate-assisted insertion device of the utility model is shown. DETAILED DESCRIPTION

[0027] The following description is used to disclose the utility model so that those skilled in the art can implement the utility model. The preferred embodiments described below are only examples, and those skilled in the art can think of other obvious variations. The basic principles of the utility model defined in the following description can be applied to other embodiments, variations, improvements, equivalents, and other technical solutions that do not deviate from the spirit and scope of the utility model.

[0028] Those skilled in the art should understand that, in the disclosure of the present invention, the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms should not be understood as limiting the present invention.

[0029] It is to be understood that the term "one" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element can be one, while in another embodiment, the number of the element can be multiple, and the term "one" cannot be understood as a limitation on the number. The affinity chromatography column 900 can be placed on the sieve plate 800 on the sieve plate auxiliary insertion device by negative pressure adsorption, and the sieve plate 800 is placed at the bottom of the affinity chromatography column 900. Through the sieve plate auxiliary insertion device, the user does not need to manually place the sieve plate 800 from the upper end opening of the affinity chromatography column 900 to the bottom of the affinity chromatography column 900. The process of inserting the sieve plate 800 is fast, the force is uniform, the operation is simple, and the stability is high.

[0030] refer to Figures 1 to 4 The sieve plate auxiliary placement device includes a sieve plate mold 10 and a supporting member 20, and the sieve plate mold 10 is horizontally mounted on the supporting member 20. The sieve plate mold 10 forms a plurality of evenly distributed first holes 101, and the aperture of each first hole 101 is equal to the diameter of the sieve plate 800, so that each sieve plate 800 just falls into each first hole 101. The supporting member 20 is implemented as a plate member, and the supporting member 20 defines an upper surface 21 and a lower surface 22. The sieve plate mold 10 is closely mounted on the upper surface 21, and the first holes 101 of the sieve plate mold 10 and the upper surface 21 form a placement cavity 102, and the sieve plate 800 is horizontally placed in the placement cavity 102 as a standby. The upper surface 21 of the supporting member 20 supports the sieve plate mold 10 to prevent the sieve plate 800 from falling from the first hole 101 during the process of placing the sieve plate 800 .

[0031] Those skilled in the art can understand that the sieve plate 800 is made of special raw materials through sintering and has hydrophilicity, so the sieve plate 800 needs to be stored in a dry environment. Preferably, the support member 20 is implemented as a plate member with a grid or through holes, and a plurality of channels are formed between the upper surface 21 and the lower surface 22, so that water is not easy to accumulate in the placement cavity 102 and air circulation is maintained, so that the sieve plate 800 can be stored in the placement cavity 102 in a dry state, thereby preventing the sieve plate 800 from failing due to moisture.

[0032] The affinity chromatography column 900 includes an adsorption disk 901 and a connecting rod 902, and the connecting rod 902 is inserted into the interior of the affinity chromatography column 900 so as to be movable up and down. The connecting rod 902 defines a first end and a second end, and the connecting rod 902 is implemented as a hollow tube, the first end forms a first port 90201, and the second end forms a second port 90202, and a channel is formed between the first port 90201 and the second port 90202 for gas exchange. The second end vertically passes through a circular ring and is fixedly connected to the circular ring, and the adsorption disk 901 is horizontally connected to the circular ring and is located below the circular ring at a distance, at which time a cavity 9001 is formed between the circular ring and the adsorption disk 901, and the side wall of the cavity 9001 is sealed. The first port 90201 is connected to a negative pressure pump or other air suction device, the second port 90202 extends into the cavity 9001 for a distance and is connected to the cavity 9001, and the first port 90201 and the second port 90202 are connected, so that the air in the cavity 9001 can be sucked by the negative pressure pump connected to the first port 90201.

[0033] It should be noted that when the connecting rod 902 is fully inserted into the affinity chromatography column 900, the first end portion extends a certain distance out of the upper end portion of the affinity chromatography column 900. In this way, the user can pull the first end portion up and down to enable the connecting rod 902 to drive the adsorption disk 901 to move in the axial direction of the connecting rod 902.

[0034] The adsorption disk 901 is implemented as an annular circular plate having a plurality of evenly distributed adsorption holes 90101. Preferably, the diameter of the adsorption disk 901 is consistent with the inner diameter of the affinity chromatography column 900, and is also consistent with the inner diameter of the placement chamber 102, so that the contact area between the adsorption disk 901 and the sieve plate 800 can be maximized as much as possible, so that the sieve plate 800 can be more stably adsorbed and the sieve plate 800 can be stably placed at the bottom of the affinity chromatography column 900.

[0035] Specifically, the user vertically inserts the affinity chromatography column 900 into the placement cavity 102, and the sieve plate 800 placed in the placement cavity 102 fits with the adsorption disk 901, and the sieve plate 800 blocks the adsorption holes 90101 on the adsorption disk 901, and the cavity 9001 is in a closed state. The motor drives the negative pressure pump connected to the first port 90201, so that the air in the cavity 9001 is sucked away by the negative pressure pump through the channel between the first port 90201 and the second port 90202, so that the cavity 9001 forms a vacuum environment, and negative pressure is formed on the adsorption disk 901, so that the sieve plate 800 is stably adsorbed to the adsorption disk 901. The user continues to apply uniform upward force to the first end of the connecting rod 902 to stretch the connecting rod 902, so that the sieve plate 800 adsorbed on the adsorption disk 901 is uniformly placed at the bottom of the affinity chromatography column 900. When the sieve plate 800 contacts the bottom of the affinity chromatography column 900, the upward stretching of the connecting rod 902 is stopped and the negative pressure pump is turned off. After the air enters the cavity 9001, the sieve plate 800 is released from the adsorption disk 901, and then the connecting rod 902 and the adsorption disk 901 are taken out. In this way, the sieve plate 800 can be stably and accurately placed at the bottom of the affinity chromatography column 900 without flipping or offsetting.

[0036] Furthermore, the sieve plate auxiliary insertion device further includes a positioning member 30, which is implemented as a plate with a certain thickness and forms a plurality of evenly distributed second holes 301 having the same aperture as the first holes 101 of the sieve plate mold 10. The positioning member 30 is tightly mounted on the side of the sieve plate mold 10 opposite to the supporting member 20, and the second holes 301 correspond to the first holes 101 one by one, so that the affinity chromatography column 900 can be further inserted into the first holes 101 through the second holes 301, thereby adsorbing the sieve plate 800 in the placement cavity 102, so that the affinity chromatography column 900 can be more stably inserted into the placement cavity 102 without displacement.

[0037] Preferably, the sieve plate auxiliary placement device further comprises a placement member 40, and the placement member 40 is connected to the lower surface 22 of the support member 20, so that the sieve plate auxiliary placement device can be more stably placed on the placement plane. In this embodiment, the placement member 40 is implemented as a frame with a certain height, so that there is a certain distance between the lower surface 22 and the placement plane, and sufficient air can enter the placement cavity 102 from the lower surface 22, thereby keeping the placement cavity 102 dry.

[0038] Preferably, the positioning member 30 is implemented as a magnetically absorbable material, and the sieve plate mold 10 is a magnetic material, so that the positioning member 30 is detachably mounted on the sieve plate mold 10. When the user needs to place the sieve plate 800 in the placement cavity 102, the positioning member 30 can be directly removed from the sieve plate mold 10, which is simple to operate.

[0039] Preferably, the placement piece 40 is implemented as a magnetically attractive material, so that the placement piece 40 of one sieve plate auxiliary insertion device can be magnetically connected to the positioning piece 30 of another sieve plate auxiliary insertion device, thereby achieving the purpose of stacking and placing multiple sieve plate auxiliary insertion devices for standby use.

[0040] It should be understood by those skilled in the art that the embodiments of the present invention described above and shown in the accompanying drawings are only examples and do not limit the present invention. The advantages of the present invention have been fully and effectively realized. The functions and structural principles of the present invention have been demonstrated and explained in the embodiments, and the embodiments of the present invention may be deformed or modified in any way without departing from the principles.

Claims

1. A sieve plate assisted insertion device, characterized in that: The sieve plate auxiliary insertion device comprises: A sieve plate mold, wherein the sieve plate mold forms a plurality of evenly distributed first holes; A supporting member defines an upper surface and a lower surface, the sieve plate mold is tightly mounted on the upper surface, and the first hole and the upper surface form a placement cavity.

2. The sieve plate auxiliary insertion device according to claim 1, characterized in that: The sieve plate auxiliary placement device also includes a positioning member, which is implemented as a plate with a certain thickness and forms a plurality of evenly distributed second holes with the same diameter as the first holes of the sieve plate mold.

3. The sieve plate auxiliary insertion device according to claim 2, characterized in that: The sieve plate auxiliary placement device also includes a placement piece, which is connected to the lower surface of the supporting member.

4. The sieve plate auxiliary insertion device according to claim 3, characterized in that: The positioning member is closely mounted on a side of the sieve plate mold opposite to the supporting member, and the second holes correspond to the first holes one by one.

5. The sieve plate auxiliary insertion device according to claim 4, characterized in that: The positioning piece is implemented as a magnetically attractable material, the object placement piece is implemented as a magnetically attractable material, and the object placement piece and the positioning piece are connected in a magnetically attractable manner.

6. The sieve plate auxiliary insertion device according to claim 3, characterized in that: The storage element is implemented as a frame with a certain height.

7. The sieve plate auxiliary insertion device according to claim 1, characterized in that: The supporting member is implemented to have a grid or mesh, and a channel for air circulation is formed between the upper surface and the lower surface.

8. The sieve plate auxiliary insertion device according to claim 1, characterized in that: The placement cavity is adapted to the outer diameter of the affinity chromatography column.

9. The sieve plate auxiliary insertion device according to claim 1, characterized in that: The screen plate is placed horizontally in the placement cavity.

10. The sieve plate auxiliary insertion device according to claim 8, characterized in that: The affinity chromatography column is vertically inserted into the placement chamber.

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