T lymphocyte efficient separation kit

Abstract

The utility model discloses an efficient T lymphocyte separation kit which is characterized in that a separation frame is connected in a kit body, a plurality of groups of reagent bodies are arranged in the separation frame, and a kit cover is connected to the top of the kit body through a hinge; according to the efficient T lymphocyte separation kit, a separation frame can extrude a first spring when moving downwards, the first spring generates counter-acting force through self material deformation when being extruded, vibration impact of the separation frame is reduced, protection on cells in a reagent body is improved, and an air cushion can be extruded when expanding to the inner wall of the reagent box body; friction force can be generated when an air cushion is extruded, so that the sliding speed of the separation frame is reduced, protection on cells in the reagent bodies is further improved, finally, a rubber pad can be reset through self-elasticity of a second spring, the side faces of the reagent bodies can be extruded and positioned when the rubber pad is reset, collision between different reagent bodies is reduced, and the stability of the reagent bodies is improved. Therefore, cross contamination between cells is prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation kits, in particular to a T lymphocyte efficient separation kit. Background Art

[0002] The cell separation kit is a tool used to separate and purify cell samples from a mixture. It contains reagents, buffers and other necessary materials to provide a convenient, fast and efficient cell separation solution. The separation methods include density gradient centrifugation, immunomagnetic bead separation and cell screening. T lymphocytes, also known as T cells, are white blood cells in the immune system. They can produce cytokines to regulate immune responses and kill infected cells by cooperating with other cells, and play an important role in the function and disease of the immune system.

[0003] In the prior art, a Chinese patent with authorization announcement number CN217922064U discloses an auxiliary tumor-infiltrating T lymphocyte separation kit, which belongs to the field of cell separation kits. Its technical highlights include a kit body, a fixed base is arranged below the kit body, and a pipette auxiliary bracket is fixedly connected to the fixed base. The pipette auxiliary bracket is arranged on one side of the kit body, and the pipette auxiliary bracket includes a pipette sheath. The pipette sheath is provided with a collection space that allows the pipette to partially pass through. By inserting the pipette into the pipette sheath, the pipette head passes through the sheath, and the handle part cannot pass through the collection space, so that the experimenter does not have to rely entirely on his own strength when using it, which reduces the jitter or collection error when using the pipette and reduces the work intensity of the experimenter.

[0004] At present, most cell separation kits on the market still have certain deficiencies when used. As shown in the above-mentioned document, the device can make it easier to extract cells with a pipette and is convenient to operate. However, when the kit needs to be transported, the vibration generated can easily damage the cells. Secondly, the reagent bottles inside the box are unstable and may collide and cause cross infection, which results in certain deficiencies in use. Utility Model Content

[0005] The utility model aims to provide a T lymphocyte efficient separation kit to solve the problems in the above background technology that the cell separation kit is not well protected during transportation and is easily damaged, and the reagent itself is unstable and may cause cross infection.

[0006] To achieve the above object, the utility model provides the following technical solution: a T lymphocyte efficient separation kit, wherein the reagent box is connected to a partition frame, a plurality of reagent bodies are arranged inside the partition frame, and a box cover is connected to the top of the reagent box through a hinge;

[0007] The reagent box body is provided with a first sliding groove inside, a first sliding block slides inside the first sliding groove, a shock absorbing mechanism is provided inside the reagent box body to reduce vibration impact generated when the reagent box body is transported, the shock absorbing mechanism includes a first rotating shaft, and the first rotating shaft is fixed inside the reagent box body and on the top of the first sliding block, a second sliding groove is provided at the bottom of the partition frame, a second sliding block slides inside the second sliding groove, a second rotating shaft is fixed at the bottom of the second sliding block and the bottom of the partition frame, a lifting frame is connected between the second rotating shaft and the first rotating shaft, a first spring is connected between the partition frame and the reagent box body, and an auxiliary mechanism is also provided at the bottom of the partition frame;

[0008] A telescopic slot is provided on the top of the partition frame, and a squeezing mechanism is arranged inside the telescopic slot.

[0009] Furthermore, the first sliding block and the second sliding block respectively form an integral structure with the first rotating shaft and the second rotating shaft, the first rotating shaft, the lifting frame and the second rotating shaft form a linkage structure, and the lifting frame has two groups symmetrically front and back inside the reagent box.

[0010] Furthermore, the partition frame and the reagent box body form a telescopic structure through a first spring, and the first spring is distributed in a bilaterally symmetrical manner inside the reagent box body.

[0011] Furthermore, the auxiliary mechanism includes an airbag, which is connected between the partition frame and the reagent box. Placement grooves are opened on both sides of the partition frame. Air cushions are connected inside the placement grooves. A connecting tube is connected between the air cushion and the airbag.

[0012] Furthermore, the extrusion mechanism includes a second spring, and the second spring is connected to the inside of the telescopic slot, and the end of the second spring is connected to the telescopic block.

[0013] Furthermore, the telescopic block penetrates and slides inside the telescopic slot, an extrusion block is fixed on the top of the telescopic block, and a rubber pad is connected to the side of the extrusion block.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] 1. The T lymphocyte efficient separation kit can squeeze the first spring when the partition frame moves downward. When the first spring is squeezed, a reaction force is generated by the deformation of its own material, thereby reducing the vibration impact of the partition frame and improving the protection of the cells inside the reagent body. The air cushion can be squeezed when it expands to the inner wall of the reagent box. When the air cushion is squeezed, friction can be generated to reduce the speed of the partition frame when it slides down, further improving the protection of the cells inside the reagent body. Finally, the rubber pad can be reset by the elastic force of the second spring itself. When the rubber pad is reset, it can squeeze and position the side of the reagent body, thereby reducing the collision between different reagent bodies, thereby preventing cross contamination between cells.

[0016] 2. A lifting frame is provided, through which the partition frame can move up and down inside the reagent box. Compared with other shock absorption methods, it can disperse the vibration impact more effectively, has better stability, and provides better protection for the cells inside the reagent body;

[0017] 3. A first spring is provided, and the reaction force generated by the compression deformation of the first spring can attenuate the impact of the partition frame when it vibrates. The first spring is made of simple material and has low production cost, which saves expenses;

[0018] 4. An air cushion is provided, and gas is delivered to the air cushion through the air bag. The air cushion can expand and squeeze the inner wall of the reagent box, thereby further attenuating the impact caused by the vibration of the partition frame, and further improving the protection of the cells inside the reagent body;

[0019] 5. A rubber pad is provided. When the rubber pad is squeezed on the side of the reagent body, it can be deformed to increase the friction force, thereby improving the stability of the extrusion of the reagent body and preventing the reagent body from shaking strongly and affecting the environment of the internal cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall front-view three-dimensional structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the cross-sectional three-dimensional structure of the reagent box of the utility model;

[0022] Figure 3 This is an enlarged upward-looking three-dimensional structural schematic diagram of the partition frame of the utility model;

[0023] Figure 4 This is an enlarged three-dimensional structural diagram of the lifting frame of the utility model;

[0024] Figure 5 This is a schematic diagram of the split sectional three-dimensional structure of the utility model partition frame;

[0025] Figure 6 It is an enlarged three-dimensional structural schematic diagram of the extrusion block of the utility model.

[0026] In the figure: 1. reagent box; 2. partition frame; 3. reagent body; 4. box cover; 101. first sliding groove; 102. first sliding block; 103. first rotating shaft; 104. second sliding groove; 105. second sliding block; 106. second rotating shaft; 107. lifting frame; 108. first spring; 109. air bag; 110. placement slot; 111. air cushion; 112. connecting pipe; 201. telescopic groove; 202. second spring; 203. telescopic block; 204. extrusion block; 205. rubber pad. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0028] Embodiment 1:

[0029] like Figure 1-Figure 4 The technical solution shown in the figure, the present invention provides the following technical solution: In order to solve the problem that the cells inside the reagent body 3 may be damaged due to severe vibration during the transportation of the reagent kit, a shock absorbing mechanism is disclosed:

[0030] The reagent box body 1 is connected to a partition frame 2, and a plurality of reagent bodies 3 are arranged inside the partition frame 2. A box cover 4 is connected to the top of the reagent box body 1 through a hinge. A first sliding groove 101 is opened inside the reagent box body 1, and a first sliding block 102 slides inside the first sliding groove 101. A shock absorbing mechanism is arranged inside the reagent box body 1 to reduce the vibration and impact generated when the reagent box body 1 is transported. The shock absorbing mechanism includes a first rotating shaft 103, and the first rotating shaft 103 is fixed inside the reagent box body 1 and on the top of the first sliding block 102. A second sliding groove 104 is provided at the bottom of the partition frame 2, a second sliding block 105 slides inside the second sliding groove 104, a second rotating shaft 106 is fixed to the bottom of the second sliding block 105 and the bottom of the partition frame 2, a lifting frame 107 is connected between the second rotating shaft 106 and the first rotating shaft 103, a first spring 108 is connected between the partition frame 2 and the reagent box 1, an auxiliary mechanism is also provided at the bottom of the partition frame 2, a telescopic groove 201 is provided at the top of the partition frame 2, and an extrusion mechanism is provided inside the telescopic groove 201.

[0031] The first sliding block 102 and the second sliding block 105 form an integral structure with the first rotating shaft 103 and the second rotating shaft 106 respectively. The first rotating shaft 103, the lifting frame 107 and the second rotating shaft 106 form a linkage structure. The lifting frame 107 has two groups of symmetry in the front and back inside the reagent box body 1. The partition frame 2 forms a telescopic structure with the reagent box body 1 through the first spring 108. The first spring 108 is symmetrically distributed in the left and right inside the reagent box body 1. When the reagent kit is transported and vibrated, the partition frame 2 can squeeze the lifting frame 107 downward. When the lifting frame 107 is squeezed, it can rotate with the first rotating shaft 103 through the second rotating shaft 106. The lifting frame 107 When rotating, the second sliding block 105 and the first sliding block 102 can be pushed to move. When the second sliding block 105 moves, it can slide through the second sliding groove 104. When the first sliding block 102 moves, it can slide through the first sliding groove 101. When the second sliding block 105 and the first sliding block 102 slide, the partition frame 2 can slide down. When the partition frame 2 moves down, it can squeeze the first spring 108. When the first spring 108 is squeezed, it generates a reaction force through the deformation of its own material. The reaction force can reduce the impact force when the partition frame 2 moves down, reduce the vibration impact of the partition frame 2, and improve the protection of the cells inside the reagent body 3.

[0032] Embodiment 2:

[0033] like Figure 4-Figure 5 The technical solution shown in the figure, the present invention provides the following technical solution: In order to solve the problem that the shock absorbing structure of the reagent box may have limited effect and strong vibration may still cause cell damage, on the basis of embodiment 1, a flip mechanism is disclosed:

[0034] The auxiliary mechanism includes an airbag 109, which is connected between the partition frame 2 and the reagent box 1. Placement grooves 110 are opened on both sides of the partition frame 2. Air cushions 111 are connected inside the placement grooves 110. A connecting tube 112 is connected between the air cushion 111 and the airbag 109. While the reagent box protects the cells inside the reagent body 3 through the shock absorbing mechanism, the partition frame 2 can squeeze the airbag 109 when it slides downward. When the airbag 109 is squeezed, it can deform and transport the internal gas to the inside of the air cushion 111 through the connecting tube 112. At the same time, when the airbag 109 is deformed, it will generate resistance to reduce the impact force of the partition frame 2 sliding down. The air cushion 111 can be expanded by the transported gas, and the air cushion 111 can be squeezed when it expands to the inner wall of the reagent box 1. When the air cushion 111 is squeezed, friction can be generated to reduce the speed of the partition frame 2 when it slides down, thereby improving the shock absorbing effect of the reagent box and further improving the protection of the cells inside the reagent body 3.

[0035] Embodiment three:

[0036] like Figure 2 and Figure 6The technical solution shown in the figure, the present invention provides the following technical solution: In order to solve the problem that the reagent body 3 is placed unstably and the collision between the reagent bodies 3 may cause cross infection, an extrusion mechanism is disclosed:

[0037] The squeezing mechanism includes a second spring 202, and the second spring 202 is connected to the inside of the telescopic slot 201, and the end of the second spring 202 is connected to a telescopic block 203, and the telescopic block 203 penetrates and slides inside the telescopic slot 201. A squeezing block 204 is fixed on the top of the telescopic block 203, and a rubber pad 205 is connected to the side of the squeezing block 204. When the reagent body 3 of the reagent kit is stored, the reagent body 3 is moved to a dedicated position inside the partition frame 2 for insertion. When the reagent body 3 is inserted, the bottom arc surface can squeeze the side of the rubber pad 205, and when the rubber pad 205 is squeezed, it can be driven by the squeezing block 204 The telescopic block 203 moves, and the telescopic block 203 can slide through the telescopic groove 201 when moving. The telescopic block 203 can squeeze the second spring 202 when sliding. After the rubber pad 205 is squeezed to a certain position, the reagent body 3 can be inserted into the specified position of the partition frame 2. At this time, the rubber pad 205 can be reset by the elastic force of the second spring 202 itself. When the rubber pad 205 is reset, the side of the reagent body 3 can be squeezed and positioned. When the reagent body 3 is positioned, it can prevent random shaking, improve the stability of the cells, reduce the collision between different reagent bodies 3, and thus prevent cross contamination between cells.

[0038] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A T lymphocyte efficient separation kit, wherein a reagent box (1) is internally connected to a partition frame (2), a plurality of reagent bodies (3) are arranged inside the partition frame (2), and a box cover (4) is connected to the top of the reagent box (1) via a hinge, characterized in that ； The reagent box (1) is provided with a first sliding groove (101) inside, a first sliding block (102) is slidable inside the first sliding groove (101), a shock absorbing mechanism is provided inside the reagent box (1) to reduce the vibration impact generated when the reagent box (1) is transported, the shock absorbing mechanism comprises a first rotating shaft (103), and the first rotating shaft (103) is fixed inside the reagent box (1) and on the top of the first sliding block (102), a second sliding groove (104) is provided at the bottom of the partition frame (2), a second sliding block (105) is slidable inside the second sliding groove (104), a second rotating shaft (106) is fixed at the bottom of the second sliding block (105) and the bottom of the partition frame (2), a lifting frame (107) is connected between the second rotating shaft (106) and the first rotating shaft (103), a first spring (108) is connected between the partition frame (2) and the reagent box (1), and an auxiliary mechanism is also provided at the bottom of the partition frame (2); A telescopic groove (201) is provided on the top of the partition frame (2), and a squeezing mechanism is provided inside the telescopic groove (201).

2. A T lymphocyte efficient separation kit according to claim 1, characterized in that: The first sliding block (102) and the second sliding block (105) respectively form an integral structure with the first rotating shaft (103) and the second rotating shaft (106); the first rotating shaft (103), the lifting frame (107) and the second rotating shaft (106) form a linkage structure; the lifting frame (107) has two groups symmetrically arranged front and back inside the reagent box (1).

3. A T lymphocyte efficient separation kit according to claim 2, characterized in that: The partition frame (2) forms a telescopic structure with the reagent box (1) via a first spring (108), and the first spring (108) is distributed in a left-right symmetrical manner inside the reagent box (1).

4. A T lymphocyte efficient separation kit according to claim 1, characterized in that: The auxiliary mechanism comprises an air bag (109), wherein the air bag (109) is connected between the partition frame (2) and the reagent box (1), and placement grooves (110) are provided on both sides of the partition frame (2), and the placement grooves (110) are connected to air cushions (111), and a connecting pipe (112) is connected between the air cushion (111) and the air bag (109).

5. A T lymphocyte efficient separation kit according to claim 1, characterized in that: The squeezing mechanism comprises a second spring (202), and the second spring (202) is connected to the inside of the telescopic slot (201), and the end of the second spring (202) is connected to a telescopic block (203).

6. A T lymphocyte efficient separation kit according to claim 5, characterized in that: The telescopic block (203) penetrates and slides inside the telescopic slot (201); an extrusion block (204) is fixed on the top of the telescopic block (203); and a rubber pad (205) is connected to the side of the extrusion block (204).

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