High-throughput drug screening device and system

Abstract

The utility model relates to a high-throughput drug screening device and system. The high-throughput drug screening device comprises a host unit, a dispersed phase liquid supply unit, a continuous phase liquid supply unit, a micropore array chip unit and a collection unit. The device has the advantages that the host unit is used for controlling the dispersed phase liquid supply unit and the continuous phase liquid supply unit to output a dispersed phase and a continuous phase, nanoliter-scale micro-droplets are formed in the micropore array chip unit, a stable microenvironment is provided, each micro-droplet contains one or more drugs, loss of compounds and exchange of the compounds among the droplets are effectively reduced, and the drug delivery efficiency is improved. According to the micro-droplet screening device, the screening accuracy and reliability are improved, different micro-droplets are freely combined in the micro-pore array chip unit and are captured in different micro-pores by the micro-pore array chip unit, the micro-droplet preparation efficiency and accuracy are improved, compound exchange among the micro-droplets is reduced, and the problem of compound exchange among the micro-droplets is solved; the collecting unit is used for collecting redundant micro-droplets, the structure is simple, and operation is easy.

Description

Technical Field

[0001] The utility model relates to the field of microfluidic technology, and in particular to a high-throughput drug screening device and system. Background Art

[0002] In the field of biological science and drug research, cell-based compound screening is a crucial link. Through the interaction between cells and compounds, researchers can understand the biological activity of compounds and develop new drugs with therapeutic effects. However, traditional screening methods, such as test tube reactions or plate experiments, although feasible to a certain extent, usually involve a large number of test samples and complex experimental operations, which are time-consuming, labor-intensive, and costly. In addition, they are difficult to meet the requirements of large-scale sample testing and have significant limitations in terms of high throughput, high efficiency, and accuracy.

[0003] With the rapid development of the biomedical field, the demand for efficient and accurate drug screening methods is growing. The development of microfluidics and microwell array chip technology has provided more possibilities for high-throughput drug screening.

[0004] High-throughput drug screening technology is an experimental method based on the molecular and cellular levels. It uses automated operating systems, sensitive and rapid detection instruments, and computer data analysis. This technology achieves rapid, accurate, and efficient screening of a large number of compounds through automated, rapid, and large-scale compound screening. This technology has been widely used in the pharmaceutical field and has played an important role in promoting the development of new drugs. However, with the continuous deepening of life science research and the growing demand for drug development, the requirements for high-throughput drug screening technology are also increasing.

[0005] Droplet microfluidics is a technology that manipulates and generates droplets at a microscale, with high flexibility and controllability. By reacting and detecting compounds and biological targets in the form of droplets in microfluidic chips, high-throughput, low-consumption and rapid drug screening can be achieved. Droplet microfluidics can encapsulate cells in tiny droplets, isolate cells from the external environment, and improve the consistency and accuracy of the experiment. In addition, this technology can also realize the parallel processing of a large number of cells, greatly improving the screening throughput. Droplet microfluidics is also easy to integrate and automate, providing a new solution for high-throughput drug screening.

[0006] However, droplet microfluidics still faces an important challenge in cell-based compound screening, namely the exchange of compounds between droplets. In droplet microfluidics systems, compound exchange is mainly achieved through the dynamic equilibrium between surfactant molecules at the droplet-oil phase interface and the reverse micelles in the oil phase. This exchange mechanism may lead to two adverse consequences on the relevant time scale of compound screening: one is the loss of compounds from the droplets, resulting in false negative results; the other is the exchange of active compounds between droplets, resulting in false positive results. In addition, the microdroplet preparation devices in the prior art are often complex in structure and large in size.

[0007] There is no effective solution to the problems existing in the related technology, such as easy loss of compounds from droplets, exchange of compounds between droplets, and complex screening devices. Utility Model Content

[0008] The purpose of the utility model is to provide a high-throughput drug screening device and system to address the deficiencies in the prior art, so as to solve the problems existing in the related art, such as easy loss of compounds from droplets, exchange of compounds between droplets, and complexity of the screening device.

[0009] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0010] The first aspect of the utility model is to provide a high-throughput drug screening device, comprising:

[0011] A host unit, the host unit is used to control and provide power to drive the generation and flow of micro-droplets;

[0012] A dispersed phase liquid supply unit, the dispersed phase liquid supply unit is connected to the main unit and is used to store the dispersed phase liquid and to flow out the dispersed phase liquid under the action of the main unit;

[0013] A continuous phase liquid supply unit, the continuous phase liquid supply unit is connected to the main unit and is used to store the continuous phase liquid and to flow out the continuous phase liquid under the action of the main unit;

[0014] A microwell array chip unit, wherein the microwell array chip unit is connected to the dispersed phase liquid supply unit and the continuous phase liquid supply unit, respectively, and is used to form microdroplets from the dispersed phase liquid delivered by the dispersed phase liquid supply unit and the continuous phase liquid delivered by the continuous phase liquid supply unit, and to combine and capture the microdroplets;

[0015] A collecting unit is connected to the microwell array chip unit and is used to collect liquid flowing out of the microwell array chip unit.

[0016] In some embodiments, the host unit includes:

[0017] A host element, the host element is used to provide power to control and drive the formation and flow of micro-droplets;

[0018] A housing element, wherein the housing element covers the host element and is used to protect the host element;

[0019] A control element, which is disposed on a side of the housing element and connected to the host element, and is used for a user to control the host element;

[0020] A display element, which is disposed on the housing element and connected to the host element, and is used to display working parameters of the host element;

[0021] A first connecting element, which is disposed on a side of the housing element and is respectively connected to the host element and the dispersed phase liquid supply unit;

[0022] A second connecting element, a plurality of the second connecting elements are arranged on the side of the shell element and are respectively connected to the host element and the continuous phase liquid supply unit.

[0023] In some embodiments, the dispersed phase liquid supply unit comprises:

[0024] a first clamping element, the first clamping element being disposed on a horizontal plane;

[0025] A dispersed phase liquid supply element, which is disposed on the first clamping element and is used to store dispersed phase liquid and flow out dispersed phase liquid under the action of the main unit;

[0026] A dispersed phase transport element, wherein the first end of the dispersed phase transport element is connected to the second end of the dispersed phase liquid supply element, and the second end of the dispersed phase transport element is connected to the microwell array chip unit, and is used to transport dispersed phase liquid into the microwell array chip unit;

[0027] A first driving element, wherein the first driving element is disposed on the first clamping element, and a second end of the first driving element is connected to a first end of the dispersed phase liquid supply element, and is used to push the dispersed phase inside the dispersed phase liquid supply element to flow out;

[0028] a third connecting element, the third connecting element being disposed at the first end of the first driving element and connected to the host unit, and being used for transmitting a control signal transmitted by the host unit;

[0029] a fourth connecting element, which is disposed at the second end of the dispersed phase transport element and is connected to the microwell array chip unit;

[0030] A first locking element, wherein the first locking element is disposed on the first clamping element and is used to lock the first end of the dispersed phase liquid supply element on the first clamping element.

[0031] In some of the embodiments, the dispersed phase liquid supply unit further comprises:

[0032] A third locking element is disposed at the second end of the first clamping element and locks the second end of the dispersed phase liquid supply element to prevent the second end of the dispersed phase liquid supply element from shaking.

[0033] In some of the embodiments, the dispersed phase liquid supply unit further comprises:

[0034] a first sliding element, the first sliding element being disposed on a side portion of the first clamping element;

[0035] A first stabilizing element is connected to the first driving element and is slidably connected to the first sliding element, and is used to stabilize the position and direction of the first driving element.

[0036] In some of the embodiments, the dispersed phase liquid supply unit further comprises:

[0037] A first buffer element is arranged on the inner edge surface of the first locking element and is used to buffer the force between the dispersed phase liquid supply element and the first clamping element.

[0038] In some embodiments, the continuous phase liquid supply unit comprises:

[0039] a second clamping element, the second clamping element being disposed on a horizontal plane;

[0040] A continuous phase liquid supply element, which is disposed on the second clamping element and is used to store the continuous phase liquid and to flow out the continuous phase liquid under the action of the main unit;

[0041] A continuous phase transport element, wherein the first end of the continuous phase transport element is connected to the second end of the continuous phase liquid supply element, and the second end of the continuous phase transport element is connected to the microwell array chip unit, and is used to transport the continuous phase liquid into the microwell array chip unit;

[0042] a second driving element, the second driving element being disposed on the second clamping element, the second end of the second driving element being connected to the first end of the continuous phase liquid supply element, and being used for pushing the continuous phase inside the continuous phase liquid supply element to flow out;

[0043] a fifth connecting element, the fifth connecting element being disposed at the first end of the second driving element and connected to the host unit, and being used for transmitting a control signal transmitted by the host unit;

[0044] a sixth connecting element, which is disposed at the second end of the continuous phase transport element and is connected to the microwell array chip unit;

[0045] A second locking element, wherein the second locking element is disposed on the second clamping element and is used to lock the continuous phase liquid supply element on the second clamping element.

[0046] In some of the embodiments, the continuous phase liquid supply unit further comprises:

[0047] A fourth locking element is disposed at the second end of the second clamping element and locks the second end of the continuous phase liquid supply element to prevent the second end of the continuous phase liquid supply element from shaking.

[0048] In some of the embodiments, the continuous phase liquid supply unit further comprises:

[0049] a second sliding element, the second sliding element being disposed on a side portion of the second clamping element;

[0050] A second stabilizing element is connected to the second driving element and is slidably connected to the second sliding element, and is used to stabilize the position and direction of the second driving element.

[0051] In some of the embodiments, the continuous phase liquid supply unit further comprises:

[0052] A second buffer element, the second buffer element is arranged on the inner edge surface of the second locking element, and is used to buffer the force between the continuous phase liquid supply element and the second clamping element.

[0053] In some embodiments, the microwell array chip unit comprises:

[0054] A chip element, wherein the chip element is used to carry micro-droplets;

[0055] A dispersed phase liquid inlet element, the dispersed phase liquid inlet element is connected to the dispersed phase liquid supply unit and is used to transport the dispersed phase transported by the dispersed phase liquid supply unit;

[0056] A dispersed phase channel element, wherein a first end of the dispersed phase channel is connected to the dispersed phase liquid inlet element and is used for flowing the dispersed phase transported by the dispersed phase liquid supply unit;

[0057] A continuous phase liquid inlet element, the continuous phase liquid inlet element is connected to the continuous phase liquid supply unit and is used to transport the continuous phase transported by the continuous phase liquid supply unit;

[0058] A continuous phase channel element, wherein a first end of the continuous phase channel element is connected to the continuous phase liquid inlet element, and a second end of the continuous phase channel element is connected to the dispersed phase channel element, and is used to form micro-droplets at the second end of the continuous phase channel element;

[0059] A micro-droplet channel element, wherein the micro-droplet channel element is connected to the second ends of the dispersed phase channel element and the continuous phase channel element respectively, and forms a plurality of transverse and longitudinal branch channels for diverting micro-droplets;

[0060] A plurality of microporous elements, wherein the plurality of microporous elements are respectively connected to the micro-droplet channel element, and each of the microporous elements is used to accommodate at least one micro-droplet;

[0061] A plurality of liquid outlet elements are respectively connected to the micro-droplet channel element and the collecting unit, and are used for discharging the micro-droplets flowing out of the micro-droplet channel element.

[0062] In some embodiments, the collection unit comprises:

[0063] A conveying element, the conveying element is in communication with the microwell array chip unit and is used to convey the liquid flowing out of the microwell array chip unit;

[0064] A collecting element is communicated with the conveying element and is used to collect and store liquid flowing out of the conveying element.

[0065] In some of the embodiments, it also includes:

[0066] A fixing unit is detachably connected to the microwell array chip unit and is used to fix the microwell array chip unit.

[0067] In some embodiments, the fixing unit includes:

[0068] A fixing element, wherein the fixing element is hollow and is used to fix the microwell array chip unit and to visualize the microwell array chip unit;

[0069] A mounting slot element, the mounting slot element is provided on the fixing element and is used to place the microwell array chip unit;

[0070] A plurality of limiting elements are arranged on the fixing element and are used to limit the position of the microwell array chip unit to prevent the microwell array chip unit from moving.

[0071] In some of the embodiments, it also includes:

[0072] A cleaning unit is removably disposed on the dispersed phase liquid supply unit or the continuous phase liquid supply unit and is detachably connected to the micropore array chip unit for cleaning the liquid surface of the micropores of the micropore array chip unit.

[0073] In some embodiments, the cleaning unit comprises:

[0074] A cleaning liquid supply element, which is detachably connected to the dispersed phase supply unit or the continuous phase supply unit and is used to output the cleaning liquid under the action of the host unit;

[0075] A cleaning liquid delivery element, wherein a first end of the cleaning liquid delivery element is in communication with the cleaning liquid supply element, and a second end of the cleaning liquid delivery element is in communication with the microwell array chip unit, and is used for delivering the cleaning liquid of the cleaning liquid supply element to the microwell array chip unit;

[0076] A seventh connecting element is disposed at the second end of the cleaning liquid delivery element and is connected to the microwell array chip unit.

[0077] The second aspect of the utility model is to provide a high-throughput drug screening system, comprising:

[0078] The high-throughput drug screening device as described in the first aspect;

[0079] A detection device, which is used to observe and detect microdroplets in the microwell array chip unit to obtain drug screening results;

[0080] The data processing device is connected to the detection device and is used to receive and analyze the data output by the detection device and output the analysis result.

[0081] The utility model adopts the above technical solution, and compared with the prior art, has the following technical effects:

[0082] The utility model discloses a high-throughput drug screening device and system, which uses a host unit to control a dispersed phase liquid supply unit and a continuous phase liquid supply unit to output a dispersed phase and a continuous phase, accurately controls the generation of nanoliter microdroplets in a microwell array chip unit, and provides a stable microenvironment, so that each microdroplet contains one or more drugs, effectively reduces the loss of compounds and the exchange of compounds between droplets, and improves the accuracy and reliability of cell-based compound screening. Different microdroplets are freely combined in the microwell array chip unit and captured in different microwells by the microwell array chip unit. The collection unit is used to collect excess microdroplets, and the microwell array chip unit is fixed by the fixing unit. The device is used for protection and fixation, thereby improving the efficiency and accuracy of micro-droplet preparation. At the same time, the cleaning unit is used to reduce the concentration of surfactant molecules through oil washing and other methods, thereby reducing the exchange of compounds between droplets and solving the problem of compound exchange between droplets. The device has a simple structure and is easy to operate, which enables high-throughput screening of drug combinations and greatly improves the screening efficiency and the accuracy of screening results. The screening results can be recorded by a detection device, and the screening results can be analyzed by a data processing device to evaluate key indicators such as activity and toxicity of each drug combination. By comparing the screening results of different combinations, potential drug combinations can be determined, providing strong support for drug research and development. BRIEF DESCRIPTION OF THE DRAWINGS

[0083] Figure 1 1 is a schematic diagram of a high-throughput drug screening device according to an embodiment of the utility model;

[0084] Figure 2 is a schematic diagram of a host unit according to an embodiment of the utility model;

[0085] Figure 3 1 is a schematic diagram of a dispersed phase liquid supply unit according to an embodiment of the utility model;

[0086] Figure 4 1 is a schematic diagram of a continuous phase liquid supply unit according to an embodiment of the utility model;

[0087] Figure 5 is a schematic diagram of a microwell array chip unit according to an embodiment of the utility model;

[0088] Figure 6 is a schematic diagram of a collection unit according to an embodiment of the utility model;

[0089] Figure 7 is a schematic diagram of a dispersed phase liquid supply unit according to an embodiment of the utility model (II);

[0090] Figure 8 2 is a schematic diagram of a continuous phase liquid supply unit according to an embodiment of the utility model;

[0091] Fig. 9 2 is a schematic diagram of a high-throughput drug screening device according to an embodiment of the utility model;

[0092] Fig.10 is a schematic diagram of a fixing unit according to an embodiment of the utility model;

[0093] Fig.11 Schematic diagram of a high-throughput drug screening device according to an embodiment of the utility model (III);

[0094] Fig.12 is a schematic diagram of a cleaning unit according to an embodiment of the utility model;

[0095] Fig.13 It is a schematic diagram of a high-throughput drug screening system according to an embodiment of the utility model.

[0096] The accompanying drawings are denoted as follows:

[0097] 1000. High-throughput drug screening device;

[0098] 1100, host unit; 1101, host component; 1102, housing component; 1103, control component; 1104, display component; 1105, first connection component; 1106, second connection component;

[0099] 1200, dispersed phase liquid supply unit; 1201, first clamping element; 1202, dispersed phase liquid supply element; 1203, dispersed phase conveying element; 1204, first driving element; 1205, third connecting element; 1206, fourth connecting element; 1207, first locking element; 1208, third locking element; 1209, first sliding element; 1210, first stabilizing element; 1211, first buffer element;

[0100] 1300, continuous phase liquid supply unit; 1301, second clamping element; 1302, continuous phase liquid supply element; 1303, continuous phase conveying element; 1304, second driving element; 1305, fifth connecting element; 1306, sixth connecting element; 1307, second locking element; 1308, fourth locking element; 1309, second sliding element; 1310, second stabilizing element; 1311, second buffer element;

[0101] 1400, microwell array chip unit; 1401, chip element; 1402, dispersed phase inlet element; 1403, dispersed phase channel element; 1404, continuous phase inlet element; 1405, continuous phase channel element; 1406, microdroplet channel element; 1407, microwell element; 1408, liquid outlet element;

[0102] 1500, collecting unit; 1501, conveying element; 1502, collecting element;

[0103] 1600, fixing unit; 1601, fixing element; 1602, mounting slot element; 1603, limiting element;

[0104] 1700, cleaning unit; 1701, cleaning liquid supply element; 1702, cleaning liquid delivery element; 1703, seventh connecting element;

[0105] 2000, detection device;

[0106] 3000. Data processing device. DETAILED DESCRIPTION

[0107] In order to make the purpose, technical solutions and advantages of the present application clearer, the present application is described and illustrated below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not intended to limit the present application. Based on the embodiments provided in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present application.

[0108] Obviously, the drawings described below are only some examples or embodiments of the present application. For ordinary technicians in this field, the present application can also be applied to other similar scenarios based on these drawings without creative work. In addition, it can also be understood that although the efforts made in this development process may be complicated and lengthy, for ordinary technicians in this field related to the content disclosed in this application, some changes in design, manufacturing or production based on the technical content disclosed in this application are just conventional technical means, and should not be understood as insufficient content disclosed in this application.

[0109] Reference to "embodiments" in this application means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those of ordinary skill in the art that the embodiments described in this application may be combined with other embodiments without conflict.

[0110] Unless otherwise defined, the technical terms or scientific terms involved in this application should be understood by people with ordinary skills in the technical field to which this application belongs. The words "one", "a", "a", "the" and the like involved in this application do not indicate a quantitative limitation, and may represent the singular or plural. The terms "include", "comprise", "have" and any of their variations involved in this application are intended to cover non-exclusive inclusions; for example, a process, method, system, product or device that includes a series of steps or units (units) is not limited to the listed steps or units, but may also include steps or units that are not listed, or may also include other steps or units inherent to these processes, methods, products or devices. The words "connect", "connected", "coupled" and the like involved in this application are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. The "multiple" involved in this application refers to two or more. "And / or" describes the association relationship of associated objects, indicating that there can be three relationships, for example, "A and / or B" can mean: A exists alone, A and B exist at the same time, and B exists alone. The character " / " generally indicates that the objects before and after are in an "or" relationship. The terms "first", "second", "third", etc. involved in this application are only used to distinguish similar objects and do not represent a specific ordering of the objects.

[0111] Example 1

[0112] This embodiment relates to a high-throughput drug screening device.

[0113] An illustrative embodiment of the present utility model is as follows: Figure 1 As shown, a high-throughput drug screening device 1000 includes a host unit 1100, a dispersed phase liquid supply unit 1200, a continuous phase liquid supply unit 1300, a microwell array chip unit 1400 and a collection unit 1500. Among them, the host unit 1100 is used to control and provide power to drive the generation and flow of microdroplets; the dispersed phase liquid supply unit 1200 is connected to the host unit 1100, and is used to store the dispersed phase liquid and flow out the dispersed phase liquid under the action of the host unit 1100; the continuous phase liquid supply unit 1300 is connected to the host unit 1100, and is used to store the continuous phase liquid and flow out the continuous phase liquid under the action of the host unit 1100; the micropore array chip unit 1400 is connected to the dispersed phase liquid supply unit 1200 and the continuous phase liquid supply unit 1300 respectively, and is used to form microdroplets from the dispersed phase liquid transported by the dispersed phase liquid supply unit 1200 and the continuous phase liquid transported by the continuous phase liquid supply unit 1300, and to combine and capture the microdroplets; the collecting unit 1500 is connected to the micropore array chip unit 1400, and is used to collect the liquid flowing out of the micropore array chip unit 1400.

[0114] like Figure 2 As shown, the host unit 1100 includes a host element 1101 , a housing element 1102 , a manipulation element 1103 , a display element 1104 , a first connection element 1105 and a second connection element 1106 . Among them, the host element 1101 is used to provide power to control and drive the formation and flow of micro-droplets; the shell element 1102 covers the host element 1101 to protect the host element 1101; the control element 1103 is arranged on the side of the shell element 1102 and connected to the host element 1101, and is used for the user to control the host element 1101; the display element 1104 is arranged on the shell element 1102 and connected to the host element 1101, and is used to display the working parameters of the host element 1101; the first connecting element 1105 is arranged on the side of the shell element 1102, and is respectively connected to the host element 1101 and the dispersed phase liquid supply unit 1200; a plurality of second connecting elements 1106 are arranged on the side of the shell element 1102, and are respectively connected to the host element 1101 and the continuous phase liquid supply unit 1300.

[0115] In some of the embodiments, the host component 1101 includes but is not limited to a single-chip microcomputer, a chip, and a processor.

[0116] In some of the embodiments, the host component 1101 can precisely control the generation of microdroplets and provide a stable microenvironment.

[0117] The size of the housing element 1102 matches the size of the host element 1101. Generally, the radial size (such as outer diameter, length, width) of the housing element 1102 is larger than the radial size (such as outer diameter, length, width) of the host element 1101, and the height of the housing element 1102 is larger than the height of the host element 1101.

[0118] In some of the embodiments, the material of the housing element 1102 includes but is not limited to plastic.

[0119] In some of the embodiments, the housing element 1102 is a protective shell.

[0120] The size of the control element 1103 matches the size of the housing element 1102. Generally, the radial size (such as outer diameter, length, width) of the control element 1103 is smaller than the radial size (such as length, width) of the cross section of the housing element 1102 where the control element 1103 is located.

[0121] In some embodiments, there are multiple operating elements 1103 , which are spaced apart along the width direction and / or length direction of the housing element 1102 .

[0122] In some of the embodiments, the control element 1103 includes but is not limited to a power key, an adjustment key, and a confirmation key.

[0123] The size of the display element 1104 matches the size of the housing element 1102. Generally, the radial size (eg, length, width) of the display element 1104 is smaller than the radial size (eg, length, width) of the housing element 1102.

[0124] In some embodiments, the display element 1104 includes but is not limited to a liquid crystal display.

[0125] The first connection element 1105 and the housing element 1102 are connected in a fixed connection or a detachable connection. The fixed connection includes but is not limited to one-piece molding; the detachable connection includes but is not limited to threaded connection and plug-in.

[0126] The size of the first connecting element 1105 matches the size of the housing element 1102. Generally, the radial size (such as outer diameter) of the first connecting element 1105 is smaller than the radial size (such as length, width) of the cross section of the housing element 1102 where the first connecting element 1105 is located.

[0127] In some of the embodiments, the first connection element 1105 is a first connection interface.

[0128] The second connection element 1106 and the housing element 1102 are connected in a fixed connection or a detachable connection. The fixed connection includes but is not limited to one-piece molding; the detachable connection includes but is not limited to threaded connection and plug-in.

[0129] The size of the second connecting element 1106 matches the size of the housing element 1102. Generally, the radial size (eg, outer diameter) of the second connecting element 1106 is smaller than the radial size (eg, length, width) of the cross section of the housing element 1102 where the second connecting element 1106 is located.

[0130] In some of the embodiments, the second connection element 1106 is a second connection interface.

[0131] like Figure 3As shown, the dispersed phase liquid supply unit 1200 includes a first clamping element 1201, a dispersed phase liquid supply element 1202, a dispersed phase conveying element 1203, a first driving element 1204, a third connecting element 1205, a fourth connecting element 1206 and a first locking element 1207. The first clamping element 1201 is arranged on a horizontal plane; the dispersed phase liquid supply element 1202 is arranged on the first clamping element 1201, and is used to store the dispersed phase liquid and flow out the dispersed phase liquid under the action of the host unit 1100; the first end of the dispersed phase conveying element 1203 is connected to the second end of the dispersed phase liquid supply element 1202, and the second end of the dispersed phase conveying element 1203 is connected to the microwell array chip unit 1400, and is used to convey the dispersed phase liquid into the microwell array chip unit 1400; the first driving element 1204 is arranged on the first clamping element 1201, and the second end of the first driving element 1204 is connected to the microwell array chip unit 1400. The first end of the dispersed phase liquid supply element 1202 is connected to promote the dispersed phase inside the dispersed phase liquid supply element 1202 to flow out; the third connecting element 1205 is arranged at the first end of the first driving element 1204 and is connected to the main unit 1100, and is used to transmit the control signal transmitted by the main unit 1100; the fourth connecting element 1206 is arranged at the second end of the dispersed phase transport element 1203 and is connected to the micropore array chip unit 1400; the first locking element 1207 is arranged on the first clamping element 1201, and is used to lock the first end of the dispersed phase liquid supply element 1202 to the first clamping element 1201.

[0132] Specifically, the third connecting element 1205 is connected to the first connecting element 1105 .

[0133] In some of the embodiments, the material of the first clamping element 1201 includes but is not limited to plastic.

[0134] In some of the embodiments, the first clamping element 1201 is a first clamping seat.

[0135] In some embodiments, the first clamping element 1201 includes a first base, a first groove and a first notch, wherein the first base is disposed on a horizontal plane; the first groove is disposed on the first base, and the dispersed phase liquid supply element 1202 is removably disposed therein; the first notch is disposed at the second end of the first base and communicates with the first groove, so as to expose the second end of the dispersed phase liquid supply element 1202.

[0136] The dispersed phase liquid supply element 1202 and the first clamping element 1201 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to a snap connection.

[0137] The size of the dispersed phase supply element 1202 matches the size of the first clamping element 1201. Generally, the radial size (such as outer diameter, length, width) of the dispersed phase supply element 1202 is smaller than the radial size (such as inner diameter, length, width) of the first clamping element 1201.

[0138] In some of the embodiments, the dispersed phase liquid supply element 1202 is a dispersed phase syringe. Specifically, the dispersed phase liquid supply element 1202 includes a dispersed phase injection cylinder and a dispersed phase injection piston. The first end of the dispersed phase injection cylinder is detachably connected to the first groove of the first clamping element 1201, and the second end of the dispersed phase injection cylinder is connected to the first end of the dispersed phase conveying element 1203, which is used to store the dispersed phase liquid and convey the dispersed phase liquid to the dispersed phase conveying element 1203; the dispersed phase injection piston is detachably connected to the first driving element 1204, which is used to push the dispersed phase liquid inside the dispersed phase injection cylinder to flow to the dispersed phase conveying element 1203 under the action of the first driving element 1204.

[0139] The dispersed phase delivery element 1203 and the dispersed phase liquid supply element 1202 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to a threaded connection and a clamping connection.

[0140] The size of the dispersed phase transport element 1203 matches that of the dispersed phase supply element 1202. Generally, the radial dimension (eg, outer diameter) of the dispersed phase transport element 1203 is not greater than the radial dimension (eg, outer diameter) of the second end of the dispersed phase supply element 1202.

[0141] In some of the embodiments, the material of the dispersed phase transport element 1203 includes but is not limited to quartz.

[0142] In some of the embodiments, the dispersed phase transport element 1203 is a first capillary tube.

[0143] The size of the first driving element 1204 matches the size of the first clamping element 1201. Generally, the radial size (such as outer diameter, length, width) of the first driving element 1204 is smaller than the radial size (such as outer diameter, length, width) of the first clamping element 1201, and the axial size (such as length) of the first driving element 1204 is smaller than the axial size (such as length) of the first clamping element 1201.

[0144] In some embodiments, the first driving element 1204 includes a first driving motor, a first driving shaft and a first driving baffle. The first driving motor is disposed at the first base of the first clamping element 1201 and connected to the third connecting element 1205; the first end of the first driving shaft is connected to the first driving motor and is used to move under the action of the first driving motor; the first driving baffle is disposed at the second end of the first driving shaft and abuts against the dispersed phase injection piston of the dispersed phase liquid supply element 1202, and is used to push the dispersed phase injection piston to move along the axial direction of the dispersed phase injection cylinder under the action of the first driving shaft.

[0145] In some of the embodiments, the first driving shaft is a telescopic shaft, which is used to be telescopic under the action of the first driving motor.

[0146] The third connection element 1205 is connected to the first connection element 1105 in a detachable manner, wherein the detachable connection manner includes but is not limited to plugging.

[0147] The size of the third connecting element 1205 matches the size of the first clamping element 1201. Generally, the radial size (such as outer diameter) of the third connecting element 1205 is smaller than the radial size (such as outer diameter, length, width) of the first clamping element 1201.

[0148] In some embodiments, the third connection element 1205 includes a third connection interface and a first connection line, wherein the third connection interface is provided on the first drive motor; and the first connection line is connected to the third connection interface and the first connection element 1105 respectively.

[0149] In some embodiments, the first connection line includes but is not limited to a signal transmission line.

[0150] The fourth connecting element 1206 is connected to the dispersed phase transport element 1203 in a detachable manner, wherein the detachable connection manner includes but is not limited to plug-in connection.

[0151] The size of the fourth connecting element 1206 matches the size of the dispersed phase transport element 1203. Generally, the radial size (such as outer diameter) of the fourth connecting element 1206 is not less than the radial size (such as outer diameter, length, width) of the dispersed phase transport element 1203.

[0152] In some of the embodiments, the fourth connection element 1206 is a fourth connection interface.

[0153] The first locking element 1207 is connected to the dispersed phase liquid supply element 1202 in a detachable manner, wherein the detachable connection manner includes but is not limited to a snap connection.

[0154] The size of the first locking element 1207 matches the size of the first clamping element 1201. Generally, the outer diameter of the first locking element 1207 is smaller than the outer diameter of the first clamping element 1201 and larger than the inner diameter of the first clamping element 1201, and the width of the first locking element 1207 is smaller than the length of the first clamping element 1201.

[0155] The size of the first locking element 1207 matches the size of the dispersed phase supply element 1202. Generally, the outer diameter of the first locking element 1207 is larger than the outer diameter of the connection part of the dispersed phase supply element 1202, and the width of the first locking element 1207 is larger than the thickness of the connection part of the dispersed phase supply element 1202.

[0156] In some of the embodiments, the first locking element 1207 is a first slot.

[0157] like Figure 4 As shown, the continuous phase liquid supply unit 1300 includes a second clamping element 1301, a continuous phase liquid supply element 1302, a continuous phase conveying element 1303, a second driving element 1304, a fifth connecting element 1305, a sixth connecting element 1306, and a second locking element 1307. The second clamping element 1301 is arranged on a horizontal plane; the continuous phase liquid supply element 1302 is arranged on the second clamping element 1301, and is used to store the continuous phase liquid and flow out the continuous phase liquid under the action of the host unit 1100; the first end of the continuous phase conveying element 1303 is connected to the second end of the continuous phase liquid supply element 1302, and the second end of the continuous phase conveying element 1303 is connected to the micropore array chip unit 1400, and is used to convey the continuous phase liquid into the micropore array chip unit 1400; the second driving element 1304 is arranged on the second clamping element 1301, and the first end of the second driving element 1304 is connected to the micropore array chip unit 1400. The two ends are connected to the first end of the continuous phase liquid supply element 1302, and are used to push the continuous phase inside the continuous phase liquid supply element 1302 to flow out; the fifth connecting element 1305 is arranged at the first end of the second driving element 1304, and is connected to the main unit 1100, and is used to transmit the control signal transmitted by the main unit 1100; the sixth connecting element 1306 is arranged at the second end of the continuous phase conveying element 1303, and is connected to the micropore array chip unit 1400; the second locking element 1307 is arranged on the second clamping element 1301, and is used to lock the continuous phase liquid supply element 1302 in the second clamping element 1301.

[0158] Specifically, the fifth connecting element 1305 is connected to the second connecting element 1106 .

[0159] In some of the embodiments, the material of the second clamping element 1301 includes but is not limited to plastic.

[0160] In some of the embodiments, the second clamping element 1301 is a second clamping seat.

[0161] In some embodiments, the second clamping element 1301 includes a second base, a second groove, and a second notch. The second base is disposed on a horizontal plane; the second groove is disposed on the second base, and the continuous phase liquid supply element 1302 is removably disposed therein; and the second notch is disposed at the second end of the second base to expose the second end of the continuous phase liquid supply element 1302.

[0162] The continuous phase liquid supply element 1302 and the second clamping element 1301 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to a snap connection.

[0163] The size of the continuous phase supply element 1302 matches the size of the second clamping element 1301. Generally, the radial size (such as outer diameter, length, width) of the continuous phase supply element 1302 is smaller than the radial size (such as inner diameter, length, width) of the second clamping element 1301.

[0164] In some of the embodiments, the continuous phase liquid supply element 1302 is a continuous phase syringe. Specifically, the continuous phase liquid supply element 1302 includes a continuous phase injection cylinder and a continuous phase injection piston. The first end of the continuous phase injection cylinder is detachably connected to the second groove of the second clamping element 1301, and the second end of the continuous phase injection cylinder is connected to the first end of the continuous phase conveying element 1303. The continuous phase injection cylinder is used to store the continuous phase liquid and convey the continuous phase liquid to the continuous phase conveying element 1303; the continuous phase injection piston is detachably connected to the second driving element 1304, and is used to push the continuous phase liquid inside the continuous phase injection cylinder to flow to the continuous phase conveying element 1303 under the action of the second driving element 1304.

[0165] The continuous phase conveying element 1303 and the continuous phase liquid supply element 1302 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to a threaded connection and a clamping connection.

[0166] The size of the continuous phase transport element 1303 matches that of the continuous phase supply element 1302. Generally, the radial dimension (eg, outer diameter) of the continuous phase transport element 1303 is not greater than the radial dimension (eg, outer diameter) of the second end of the continuous phase supply element 1302.

[0167] In some of the embodiments, the material of the continuous phase transport element 1303 includes but is not limited to quartz.

[0168] In some of the embodiments, the continuous phase transport element 1303 is a second capillary.

[0169] The size of the second driving element 1304 matches the size of the second clamping element 1301. Generally, the radial size (such as outer diameter, length, width) of the second driving element 1304 is smaller than the radial size (such as outer diameter, length, width) of the second clamping element 1301, and the axial size (such as length) of the second driving element 1304 is smaller than the axial size (such as length) of the second clamping element 1301.

[0170] In some embodiments, the second driving element 1304 includes a second driving motor, a second driving shaft and a second driving baffle. The second driving motor is disposed on the second base of the second clamping element 1301 and connected to the fifth connecting element 1305; the first end of the second driving shaft is connected to the second driving motor and is used to move under the action of the second driving motor; the second driving baffle is disposed at the second end of the second driving shaft and abuts against the continuous phase injection piston of the continuous phase liquid supply element 1302, and is used to push the continuous phase injection piston to move along the axial direction of the dispersed phase injection cylinder under the action of the second driving shaft.

[0171] In some of the embodiments, the second driving shaft is a telescopic shaft, which is used to be telescopic under the action of the first driving motor.

[0172] The fifth connection element 1305 and the second connection element 1106 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to plugging.

[0173] The size of the fifth connecting element 1305 matches the size of the second clamping element 1301. Generally, the radial size (such as the outer diameter) of the fifth connecting element 1305 is smaller than the radial size (such as the outer diameter, length, width) of the second clamping element 1301.

[0174] In some embodiments, the fifth connection element 1305 includes a fifth connection interface and a second connection line, wherein the fifth connection interface is provided on the second drive motor; and the second connection line is connected to the fifth connection interface and the second connection element 1106 respectively.

[0175] In some embodiments, the second connection line includes but is not limited to a signal transmission line.

[0176] The sixth connecting element 1306 is connected to the continuous phase conveying element 1303 in a detachable manner, wherein the detachable connection manner includes but is not limited to plugging.

[0177] The size of the sixth connecting element 1306 matches the size of the continuous phase transport element 1303. Generally, the radial size (eg, outer diameter) of the sixth connecting element 1306 is not less than the radial size (eg, outer diameter, length, width) of the continuous phase transport element 1303.

[0178] In some of the embodiments, the sixth connection element 1306 is a sixth connection interface.

[0179] The second locking element 1307 is connected to the continuous phase liquid supply element 1302 in a detachable manner, wherein the detachable connection manner includes but is not limited to a snap connection.

[0180] The size of the second locking element 1307 matches the size of the second clamping element 1301. Generally, the outer diameter of the second locking element 1307 is smaller than the outer diameter of the second clamping element 1301 and larger than the inner diameter of the second clamping element 1301, and the width of the second locking element 1307 is smaller than the length of the second clamping element 1301.

[0181] The size of the second locking element 1307 matches the size of the continuous phase supply element 1302. Generally, the outer diameter of the second locking element 1307 is larger than the outer diameter of the connecting portion of the continuous phase supply element 1302, and the width of the second locking element 1307 is larger than the thickness of the connecting portion of the continuous phase supply element 1302.

[0182] In some of the embodiments, the second locking element 1307 is a second slot.

[0183] like Figure 5As shown, the microwell array chip unit 1400 includes a chip element 1401, a dispersed phase liquid inlet element 1402, a dispersed phase channel element 1403, a continuous phase liquid inlet element 1404, a continuous phase channel element 1405, a micro-droplet channel element 1406, a plurality of microwell elements 1407 and a plurality of liquid outlet elements 1408. Among them, the chip element 1401 is used to carry micro-droplets; the dispersed phase liquid inlet element 1402 is connected to the dispersed phase liquid supply unit 1200, and is used to transport the dispersed phase transported by the dispersed phase liquid supply unit 1200; the first end of the dispersed phase channel is connected to the dispersed phase liquid inlet element 1402, and is used to flow the dispersed phase transported by the dispersed phase liquid supply unit 1200; the continuous phase liquid inlet element 1404 is connected to the continuous phase liquid supply unit 1300, and is used to transport the continuous phase transported by the continuous phase liquid supply unit 1300; the first end of the continuous phase channel element 1405 is connected to the continuous phase liquid inlet element 1404, and the second end of the continuous phase channel element 1405 is connected to the dispersed phase channel element 1 403 is connected to form microdroplets at the second end of the continuous phase channel element 1405; the microdroplet channel element 1406 is respectively connected to the dispersed phase channel element 1403 and the second end of the continuous phase channel element 1405, and forms a plurality of transverse and longitudinal branch channels for diverting the microdroplets; a plurality of microporous elements 1407 are respectively connected to the microdroplet channel element 1406, and each microporous element 1407 is used to accommodate at least one microdroplet; the liquid outlet element 1408 is arranged on the chip element 1401, and is respectively connected to the microdroplet channel element 1406 and the collecting unit 1500, and is used to flow out the microdroplets flowing out of the microdroplet channel element 1406.

[0184] Specifically, the dispersed phase liquid inlet element 1402 is connected to the fourth connecting element 1206 ; and the continuous phase liquid inlet element 1404 is connected to the sixth connecting element 1306 .

[0185] In some of the embodiments, the material of the chip component 1401 includes but is not limited to polydimethylsiloxane.

[0186] In some of the embodiments, the chip element 1401 is a PDMS microfluidic chip.

[0187] The dispersed phase inlet element 1402 and the fourth connecting element 1206 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to plug-in connection.

[0188] The size of the dispersed phase inlet element 1402 matches the size of the fourth connecting element 1206. Generally, the radial size (eg, inner diameter) of the dispersed phase inlet element 1402 is not greater than the radial size (eg, outer diameter) of the fourth connecting element 1206.

[0189] In some of the embodiments, the dispersed phase liquid inlet element 1402 is a dispersed phase liquid inlet hole or a dispersed phase liquid inlet column.

[0190] In some of the embodiments, the size of the dispersed phase channel element 1403 is nanoscale.

[0191] In some of the embodiments, the radial dimension of the connection between the dispersed phase channel element 1403 and the continuous phase liquid inlet element 1404 gradually decreases and is tapered.

[0192] The continuous phase liquid inlet element 1404 and the sixth connecting element 1306 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to plug-in connection.

[0193] The size of the continuous phase inlet element 1404 matches the size of the sixth connecting element 1306. Generally, the radial size (eg, inner diameter) of the continuous phase inlet element 1404 is not greater than the radial size (eg, outer diameter) of the sixth connecting element 1306.

[0194] In some of the embodiments, the continuous phase liquid inlet element 1404 is a continuous phase liquid inlet hole or a continuous phase liquid inlet column.

[0195] The size of the continuous phase channel element 1405 matches the size of the dispersed phase channel element 1403. Generally, the radial size (eg, inner diameter) of the continuous phase channel element 1405 is equal to the radial size (eg, inner diameter) of the dispersed phase channel element 1403.

[0196] In some of the embodiments, the continuous phase channel element 1405 is nanometer-sized to generate nanoliter-scale droplets.

[0197] In some of the embodiments, the continuous phase channel element 1405 and the dispersed phase channel element 1403 form a T-shaped channel.

[0198] The size of the microdroplet channel element 1406 matches the size of the continuous phase channel element 1405. Generally, the radial dimension (eg, inner diameter) of the microdroplet channel element 1406 is not greater than the radial dimension (eg, inner diameter) of the continuous phase channel element 1405.

[0199] In some embodiments, the micro-droplet channel element 1406 includes a main channel, a plurality of oblique branch channels, a plurality of longitudinal branch channels, and a plurality of transverse branch channels. The main channel is connected to the connection between the dispersed phase channel element 1403 and the continuous phase channel element 1405, and is used to transport the formed micro-droplets; a plurality of oblique branch channels are evenly distributed on both sides of the main channel and are connected to the main channel; a plurality of longitudinal branch channels are respectively connected to a plurality of oblique branch channels, microporous element 1407, and liquid outlet element 1408, and are used to transport the micro-droplets formed in the main channel to each microporous element 1407; a plurality of transverse branch channels are respectively connected to a plurality of longitudinal branch channels.

[0200] A plurality of microporous elements 1407 are arranged in an array distribution on the chip element 1401 .

[0201] The size of the microporous element 1407 matches the size of the microdroplet channel element 1406. Generally, the radial size (such as the inner diameter) of the microporous element 1407 is not less than the radial size (such as the inner diameter) of the microdroplet channel element 1406.

[0202] In some of these embodiments, microporous element 1407 is a chamber.

[0203] The connection between the liquid outlet element 1408 and the chip element 1401 is fixed connection, wherein the fixed connection includes but is not limited to integral molding.

[0204] The size of the liquid outlet element 1408 matches the size of the micro-droplet channel element 1406. Generally, the radial size (such as the inner diameter) of the liquid outlet element 1408 is not less than the radial size (such as the inner diameter) of the micro-droplet channel element 1406.

[0205] A plurality of liquid outlet elements 1408 are distributed at the end of the micro-droplet channel element 1406 .

[0206] In some of the embodiments, the liquid outlet element 1408 is a liquid outlet hole or a liquid outlet column.

[0207] like Figure 6 As shown, the collection unit 1500 includes a conveying element 1501 and a collecting element 1502. The conveying element 1501 is connected to the microwell array chip unit 1400 and is used to convey the liquid flowing out of the microwell array chip unit 1400; the collecting element 1502 is connected to the conveying element 1501 and is used to collect and store the liquid flowing out of the conveying element 1501.

[0208] Specifically, the transport element 1501 is in communication with the liquid outlet element 1408 and is used to transport excess micro-droplets flowing out of the micro-droplet channel element 1406 .

[0209] The connection between the delivery element 1501 and the liquid outlet element 1408 is a detachable connection, wherein the detachable connection includes but is not limited to a threaded connection and a plug-in connection.

[0210] The size of the delivery element 1501 matches the size of the liquid outlet element 1408. Generally, the radial size (such as the inner diameter) of the delivery element 1501 is not less than the radial size (such as the inner diameter) of the liquid outlet element 1408.

[0211] In some of the embodiments, the material of the delivery element 1501 includes but is not limited to quartz and plastic.

[0212] In some of the embodiments, the delivery element 1501 is a delivery tube.

[0213] The collection element 1502 and the delivery element 1501 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to plug-in connection and snap-in connection.

[0214] The size of the collecting element 1502 matches the size of the conveying element 1501. Generally, the radial size (eg, outer diameter) of the collecting element 1502 is larger than the radial size (eg, outer diameter) of the conveying element 1501.

[0215] In some embodiments, the material of the collection element 1502 includes but is not limited to plastic.

[0216] In some of these embodiments, collection element 1502 is a storage tank.

[0217] The use of the utility model:

[0218] (I) Installation

[0219] 1) Connecting the first connecting element 1105 to the third connecting element 1205, and connecting the second connecting element 1106 to the fifth connecting element 1305;

[0220] 2) Install the dispersed phase liquid supply element 1202 on the first clamping element 1201, and the dispersed phase conveying element 1203 is connected to the dispersed phase liquid supply element 1202 and the dispersed phase liquid inlet element 1402 respectively; install the continuous phase liquid supply element 1302 on the second clamping element 1301, and the continuous phase conveying element 1303 is connected to the continuous phase liquid supply element 1302 and the continuous phase liquid inlet element 1404 respectively;

[0221] 3) Connect the conveying element 1501 to the liquid outlet element 1408 and the collecting element 1502 respectively.

[0222] (II) Preparation of nanoliter droplets

[0223] 1) Turn on the host component 1101, and adjust the parameters displayed by the control component 1103 and the display component 1104 to control the microenvironment and the generation of microdroplets;

[0224] 2) Controlling the first driving element 1204 through the host element 1101 to push the dispersed phase of the dispersed phase liquid supply element 1202 to flow out at a set speed, and transporting it to the dispersed phase liquid inlet element 1402 through the dispersed phase conveying element 1203, and controlling the second driving element 1304 to push the continuous phase of the continuous phase liquid supply element 1302 to flow out at a set speed, and transporting it to the continuous phase liquid inlet element 1404 through the continuous phase conveying element 1303;

[0225] 3) The dispersed phase liquid flows into the dispersed phase channel element 1403, and the continuous phase liquid flows into the continuous phase channel element 1405, and forms micro-droplets at the connection point. The micro-droplets enter the micro-droplet channel element 1406 and flow to a plurality of microporous elements 1407 respectively;

[0226] 4) Excess micro-droplets flow through the liquid outlet element 1408 to the conveying element 1501 and enter the collecting element 1502 for collection and storage.

[0227] The technical effects of the utility model are as follows:

[0228] The host unit is used to control the dispersed phase supply unit and the continuous phase supply unit to output the dispersed phase and the continuous phase, and nanoliter microdroplets are formed in the microwell array chip unit to provide a stable microenvironment, so that each microdroplet contains one or more drugs, effectively reducing the loss of compounds and the exchange of compounds between droplets, and improving the accuracy and reliability of cell-based compound screening. Different microdroplets are freely combined in the microwell array chip unit and captured in different microwells by the microwell array chip unit, which improves the efficiency and accuracy of microdroplet preparation, reduces the exchange of compounds between droplets, and solves the problem of compound exchange between droplets; the collection unit is used to collect excess microdroplets, which has a simple structure and is easy to operate, and realizes high-throughput screening of drug combinations, greatly improving the screening efficiency and the accuracy of the screening results.

[0229] Example 2

[0230] This embodiment is a variation of Embodiment 1.

[0231] like Figure 7 As shown, the dispersed phase liquid supply unit 1200 further includes a third locking element 1208. The third locking element 1208 is disposed at the second end of the first clamping element 1201 and locks the second end of the dispersed phase liquid supply element 1202 to prevent the second end of the dispersed phase liquid supply element 1202 from shaking.

[0232] The third locking element 1208 is connected to the first clamping element 1201 in a rotational connection.

[0233] The size of the third locking element 1208 matches the size of the first clamping element 1201. Generally, the radial size (eg, outer diameter) of the third locking element 1208 is smaller than the radial size (eg, outer diameter, length, width) of the first clamping element 1201.

[0234] The size of the third locking element 1208 matches the size of the dispersed phase liquid supply element 1202. Generally, the radial size (such as inner diameter) of the third locking element 1208 is larger than the radial size (such as outer diameter) of the second end of the dispersed phase liquid supply element 1202, and smaller than the radial size (such as outer diameter) of the second end of the dispersed phase liquid supply element 1202.

[0235] In some embodiments, the material of the third locking element 1208 includes but is not limited to plastic and metal.

[0236] In some of the embodiments, the third locking element 1208 is a first locking buckle.

[0237] Furthermore, if Figure 7 As shown, the dispersed phase liquid supply unit 1200 further includes a first sliding element 1209 and a first stabilizing element 1210. The first sliding element 1209 is disposed on the side of the first clamping element 1201; the first stabilizing element 1210 is connected to the first driving element 1204 and is slidably connected to the first sliding element 1209, so as to stabilize the position and direction of the first driving element 1204.

[0238] The size of the first sliding element 1209 matches the size of the first clamping element 1201. Generally, the length of the first sliding element 1209 is smaller than the length of the first clamping element 1201, the width of the first sliding element 1209 is smaller than the height of the side of the first clamping element 1201, and the depth of the first sliding element 1209 is smaller than the thickness of the side of the first clamping element 1201.

[0239] In some embodiments, there are two first sliding elements 1209 , and the two first sliding elements 1209 are symmetrically disposed on both sides of the first clamping element 1201 .

[0240] In some embodiments, the first sliding element 1209 is a first sliding groove or a first sliding block.

[0241] The first stabilizing element 1210 and the first driving element 1204 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to a snap connection.

[0242] The size of the first stabilizing element 1210 matches the size of the first clamping element 1201. Generally, the radial size (such as inner diameter) of the first stabilizing element 1210 is not less than the radial size (such as outer diameter, length, width) of the first clamping element 1201, and the axial size (such as width) of the first stabilizing element 1210 is less than the axial size (such as length) of the first clamping element 1201.

[0243] In some embodiments, the material of the first stabilizing element 1210 includes but is not limited to plastic and metal.

[0244] In some embodiments, the first stabilizing element 1210 includes a first supporting element, at least one first sliding element, and a first fixing element. The first supporting element is disposed on the first clamping element 1201; the first sliding element is disposed on the side of the first clamping element and is slidably connected to the first sliding element 1209; the first fixing element is disposed on the first supporting element and is connected to the first driving element 1204 for stabilizing the first driving element 1204.

[0245] In some embodiments, there are two first sliding members, which are symmetrically disposed on both sides of the first support member and are slidably connected to the corresponding first sliding elements 1209 .

[0246] In some of the embodiments, the first sliding member is a second sliding groove or a second sliding block.

[0247] Furthermore, if Figure 7 As shown, the dispersed phase liquid supply unit 1200 further includes a first buffer element 1211 . The first buffer element 1211 is disposed on the inner edge surface of the first locking element 1207 to buffer the force between the dispersed phase liquid supply element 1202 and the first clamping element 1201 .

[0248] The first buffer element 1211 and the first clamping element 1201 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to bonding.

[0249] The size of the first buffer element 1211 matches the size of the first locking element 1207. Generally, the minimum radial dimension (such as inner diameter) of the first buffer element 1211 is larger than the minimum radial dimension (such as inner diameter) of the first locking element 1207, and the maximum radial dimension (such as outer diameter) of the first buffer element 1211 is smaller than the maximum radial dimension (such as outer diameter) of the first locking element 1207.

[0250] In some of the embodiments, the first buffer element 1211 is a first silicone buffer pad.

[0251] like Figure 8 As shown, the continuous phase liquid supply unit 1300 further includes a fourth locking element 1308. The fourth locking element 1308 is disposed at the second end of the second clamping element 1301 and locks the second end of the continuous phase liquid supply element 1302 to prevent the second end of the continuous phase liquid supply element 1302 from shaking.

[0252] The fourth locking element 1308 is connected to the second clamping element 1301 in a rotational manner.

[0253] The size of the fourth locking element 1308 matches the size of the second clamping element 1301. Generally, the radial size (such as the outer diameter) of the fourth locking element 1308 is smaller than the radial size (such as the outer diameter, length, width) of the second clamping element 1301.

[0254] The size of the fourth locking element 1308 matches the size of the continuous phase supply element 1302. Generally, the inner diameter of the fourth locking element 1308 is larger than the outer diameter of the second end of the continuous phase supply element 1302, and smaller than the outer diameter of the first end of the continuous phase supply element 1302.

[0255] In some embodiments, the material of the fourth locking element 1308 includes but is not limited to plastic and metal.

[0256] In some of the embodiments, the fourth locking element 1308 is a second locking buckle.

[0257] Furthermore, if Figure 8 As shown, the continuous phase liquid supply unit 1300 further includes a second sliding element 1309 and a second stabilizing element 1310. The second sliding element 1309 is disposed on the side of the second clamping element 1301; the second stabilizing element 1310 is connected to the second driving element 1304 and is slidably connected to the second sliding element 1309 to stabilize the position and direction of the second driving element 1304.

[0258] The size of the second sliding element 1309 matches the size of the second clamping element 1301. Generally, the length of the second sliding element 1309 is smaller than the length of the second clamping element 1301, the width of the second sliding element 1309 is smaller than the height of the side of the second clamping element 1301, and the depth of the second sliding element 1309 is smaller than the thickness of the side of the second clamping element 1301.

[0259] In some embodiments, there are two second sliding elements 1309 , and the two second sliding elements 1309 are symmetrically disposed on two sides of the second clamping element 1301 .

[0260] In some embodiments, the second sliding element 1309 is a third sliding slot or a third sliding block.

[0261] The second stabilizing element 1310 and the second driving element 1304 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to a snap connection.

[0262] The size of the second stabilizing element 1310 matches the size of the second clamping element 1301. Generally, the radial size (such as inner diameter) of the second stabilizing element 1310 is not less than the radial size (such as outer diameter, length, width) of the second clamping element 1301, and the axial size (such as width) of the second stabilizing element 1310 is less than the axial size (such as length) of the second clamping element 1301.

[0263] The size of the second stabilizing element 1310 matches the size of the second sliding element 1309. Generally, the axial size (eg, length) of the second stabilizing element 1310 is smaller than the axial size (eg, length) of the second sliding element 1309.

[0264] In some of the embodiments, the material of the second stabilizing element 1310 includes but is not limited to plastic and metal.

[0265] In some embodiments, the second stabilizing element 1310 includes a second supporting element, at least one second sliding element, and a second fixing element. The second supporting element is disposed on the second clamping element 1301; the second sliding element is disposed on the side of the second clamping element and is slidably connected to the second sliding element 1309; the second fixing element is disposed on the second supporting element and is connected to the second driving element 1304 for stabilizing the second driving element 1304.

[0266] In some embodiments, there are two second sliding members, which are symmetrically disposed on both sides of the second support member and are slidably connected to corresponding second sliding elements 1309 .

[0267] In some of the embodiments, the second sliding member is a fourth sliding slot or a fourth sliding block.

[0268] Furthermore, if Figure 8 As shown, the continuous phase liquid supply unit 1300 further includes a second buffer element 1311 . The second buffer element 1311 is disposed on the inner edge surface of the second locking element 1307 to buffer the force between the continuous phase liquid supply element 1302 and the second clamping element 1301 .

[0269] The second buffer element 1311 and the second clamping element 1301 are connected in a detachable manner, wherein the detachable connection manner includes but is not limited to bonding.

[0270] The size of the second buffer element 1311 matches the size of the second locking element 1307. Generally, the minimum radial dimension (such as inner diameter) of the second buffer element 1311 is larger than the minimum radial dimension (such as inner diameter) of the second locking element 1307, and the maximum radial dimension (such as outer diameter) of the second buffer element 1311 is smaller than the maximum radial dimension (such as outer diameter) of the second locking element 1307.

[0271] In some of the embodiments, the second cushioning element 1311 is a second silicone cushioning pad.

[0272] The method of using this embodiment is as follows:

[0273] 1) placing the dispersed phase liquid supply element 1202 into the first clamping element 1201, the first locking element 1207 fixing the first end of the dispersed phase liquid supply element 1202, and the third locking element 1208 locking the second end of the dispersed phase liquid supply element 1202; placing the continuous phase liquid supply element 1302 into the second clamping element 1301, the second locking element 1307 fixing the first end of the dispersed phase liquid supply element 1202, and the fourth locking element 1308 locking the second end of the continuous phase liquid supply element 1302;

[0274] 2) When the dispersed phase liquid supply element 1202 starts to supply liquid, the first stabilizing element 1210 slides along the first sliding element 1209, so that the first driving element 1204 stably drives the dispersed phase liquid supply element 1202 to output the dispersed phase, and uses the first buffer element 1211 to increase the friction between the dispersed phase liquid supply element 1202 and the first clamping element 1201, and at the same time buffers the action force between the dispersed phase liquid supply element 1202 and the first clamping element 1201; when the continuous phase liquid supply element 1302 starts to supply liquid, the second stabilizing element 1310 slides along the second sliding element 1309, so that the second driving element 1304 stably drives the continuous phase liquid supply element 1302 to output the continuous phase, and the second buffer element 1311 increases the friction between the continuous phase liquid supply element 1302 and the second locking element 1307, and at the same time buffers the action force between the continuous phase liquid supply element 1302 and the second clamping element 1301.

[0275] The technical effects of this embodiment are as follows:

[0276] By setting a third locking element, a first sliding element, a first stabilizing element, a fourth locking element, a second sliding element and a second stabilizing element, the positions of the dispersed phase liquid supply element and the continuous phase liquid supply element can be locked, thereby stably outputting the dispersed phase and / or the continuous phase, and the first buffer element is used to increase the friction between the dispersed phase liquid supply element and the first clamping element to buffer the force; the second buffer element is used to increase the friction between the continuous phase liquid supply element and the second clamping element to buffer the force, thereby more accurately controlling the parameters of the micro-droplets and improving the preparation accuracy and efficiency of the micro-droplets.

[0277] Example 3

[0278] This embodiment is a variation of Embodiments 1 and 2.

[0279] like Fig. 9As shown, the high-throughput drug screening device 1000 further includes a fixing unit 1600. The fixing unit 1600 is detachably connected to the microwell array chip unit 1400 and is used to fix the microwell array chip unit 1400.

[0280] like Fig.10 As shown, the fixing unit 1600 includes a fixing element 1601, a mounting groove element 1602 and a plurality of limiting elements 1603. The fixing element 1601 is hollow and used to fix the microwell array chip unit 1400 and the visible microwell array chip unit 1400; the mounting groove element 1602 is provided on the fixing element 1601 and used to place the microwell array chip unit 1400; and the plurality of limiting elements 1603 are provided on the fixing element 1601 and used to limit the position of the microwell array chip unit 1400 to prevent the microwell array chip unit 1400 from moving.

[0281] Specifically, the mounting slot component 1602 is used to place the chip component 1401 ; the limiting component 1603 is used to limit the position of the chip component 1401 .

[0282] The size of the fixing element 1601 matches the size of the chip element 1401. Generally, the length of the fixing element 1601 is greater than the length of the chip element 1401, and the width of the fixing element 1601 is greater than the width of the chip element 1401.

[0283] In some of the embodiments, the material of the fixing element 1601 includes but is not limited to plastic.

[0284] In some of the embodiments, the fixing element 1601 is a fixing plate.

[0285] The size of the mounting slot element 1602 matches the size of the fixing element 1601. Generally, the length of the mounting slot element 1602 is smaller than the length of the fixing element 1601, the width of the mounting slot element 1602 is smaller than the width of the fixing element 1601, and the depth of the mounting slot element 1602 is smaller than the thickness of the fixing element 1601.

[0286] The size of the mounting slot component 1602 matches the size of the chip component 1401. Generally, the length of the mounting slot component 1602 is not less than the length of the chip component 1401, the width of the mounting slot component 1602 is not less than the width of the chip component 1401, and the depth of the mounting slot component 1602 is not less than the thickness of the chip component 1401.

[0287] In some embodiments, there are a plurality of mounting slot components 1602. The plurality of mounting slot components 1602 are arranged in a stepped manner to mount chip components 1401 of different specifications.

[0288] In some of the embodiments, the mounting slot element 1602 is a mounting slot.

[0289] The connection mode between the limiting element 1603 and the fixing element 1601 is a rotation connection. When in use, the limiting element 1603 can be rotated to a suitable position to clamp the chip element 1401.

[0290] The size of the limiting element 1603 matches the size of the fixing element 1601. Generally, the width of the limiting element 1603 is smaller than the width of the fixing element 1601, and the length of the limiting element 1603 is smaller than the length of the fixing element 1601.

[0291] In some embodiments, the number of the limiting elements 1603 is 2. The two limiting elements 1603 are symmetrically arranged on the fixing element 1601 .

[0292] In some of the embodiments, the limiting element 1603 is an elastic clip.

[0293] The method of using this embodiment is as follows:

[0294] When in use, place the fixing element 1601 at a suitable position, then put the chip element 1401 into the mounting groove element 1602 of suitable size, fix the chip element 1401 at different positions by rotating the limiting element 1603, and then the chip element 1401 is fixed to the fixing element 1601, and the other steps are the same as in Example 1.

[0295] The technical effects of this embodiment are as follows:

[0296] By setting up the fixing unit, not only can the micropore array chip unit be protected, and the micropore array chip unit can be prevented from being rubbed and contaminated when placed directly on a horizontal surface; the position of the micropore array chip unit can also be stabilized, and the fixing of micropore array chip units of different specifications can be adapted to prevent the micropore array chip unit from moving during the preparation of microdroplets, thereby affecting the preparation results and preparation efficiency; the fixing unit is small in size and has a hollow structure, and does not affect the detection and observation of microdroplets on the micropore array chip unit.

[0297] Example 4

[0298] This embodiment is a variation of Embodiments 1 to 3.

[0299] like Fig.11 As shown, the high-throughput drug screening device 1000 further includes a cleaning unit 1700. The cleaning unit 1700 is removably disposed on the dispersed phase liquid supply unit 1200 or the continuous phase liquid supply unit 1300, and is detachably connected to the microwell array chip unit 1400, for cleaning the liquid surface of the microwells of the microwell array chip unit 1400.

[0300] like Fig.12 As shown, the cleaning unit 1700 includes a cleaning liquid supply element 1701, a cleaning liquid delivery element 1702 and a seventh connection element 1703. The cleaning liquid supply element 1701 is detachably connected to the dispersed phase supply unit 1200 or the continuous phase supply unit 1300, and is used to output the cleaning liquid under the action of the host unit 1100; the first end of the cleaning liquid delivery element 1702 is connected to the cleaning liquid supply element 1701, and the second end of the cleaning liquid delivery element 1702 is connected to the microwell array chip unit 1400, and is used to deliver the cleaning liquid of the cleaning liquid supply element 1701 to the microwell array chip unit 1400, and the seventh connection element 1703 is arranged at the second end of the cleaning liquid delivery element 1702, and is connected to the microwell array chip unit 1400.

[0301] Specifically, the cleaning liquid supply element 1701 is connected to the first clamping element 1201 and / or the second clamping element 1301, and is used to output the cleaning liquid under the action of the first driving element 1204 and / or the second driving element 1304; the cleaning liquid delivery element 1702 is connected to the dispersed phase liquid inlet element 1402 or the continuous phase liquid inlet element 1404, and is used to deliver the cleaning liquid to the microporous element 1407 and clean the droplets inside the microporous element 1407.

[0302] The connection between the cleaning liquid supply element 1701 and the first clamping element 1201 / the second clamping element 1301 is a detachable connection, wherein the detachable connection includes but is not limited to a snap connection.

[0303] The size of the cleaning liquid supply element 1701 matches the size of the first clamping element 1201 / the second clamping element 1301. Generally, the radial size (such as outer diameter, length, width) of the cleaning liquid supply element 1701 is smaller than the radial size (such as inner diameter, length, width) of the first clamping element 1201 / the second clamping element 1301.

[0304] In some of the embodiments, the cleaning liquid supply element 1701 is a cleaning liquid syringe. Specifically, the cleaning liquid supply element 1701 includes a cleaning liquid injection cylinder and a cleaning liquid injection piston. The first end of the cleaning liquid injection cylinder is detachably connected to the first groove of the first clamping element 1201 or the second groove of the second clamping element 1301, and the second end of the cleaning liquid injection cylinder is connected to the first end of the cleaning liquid delivery element 1702. The cleaning liquid injection cylinder is used to store cleaning liquid and deliver cleaning liquid to the cleaning liquid delivery element 1702; the cleaning liquid injection piston is detachably connected to the first driving element 1204 or the second driving element 1304, and is used to push the cleaning liquid inside the cleaning liquid injection cylinder to flow to the cleaning liquid delivery element 1702 under the action of the first driving element 1204 or the second driving element 1304.

[0305] The cleaning liquid delivery element 1702 is connected to the cleaning liquid supply element 1701 in a detachable manner, wherein the detachable connection manner includes but is not limited to a threaded connection and a clamping connection.

[0306] In some of the embodiments, the material of the cleaning liquid delivery element 1702 includes but is not limited to quartz.

[0307] In some embodiments, the cleaning liquid delivery element 1702 is a third capillary tube.

[0308] The seventh connection element 1703 is connected to the cleaning liquid delivery element 1702 in a detachable manner, wherein the detachable connection manner includes but is not limited to a threaded connection and a clamping connection.

[0309] The seventh connecting element 1703 is connected to the dispersed phase liquid inlet element 1402 / the continuous phase liquid inlet element 1404 in a detachable connection, wherein the detachable connection includes but is not limited to a threaded connection and a clamping connection.

[0310] In some embodiments, the material of the seventh connecting element 1703 includes but is not limited to plastic.

[0311] In some of the embodiments, the seventh connection element 1703 is a seventh connection interface.

[0312] The method of using this embodiment is as follows:

[0313] After the preparation of micro-droplets is completed, the cleaning liquid supply element 1701 is installed on the first clamping element 1201, and the seventh connecting element 1703 is connected to the dispersed phase liquid inlet element 1402 or the continuous phase liquid inlet element 1404. Then, the main component 1101 is turned on and controlled to output the cleaning liquid of the cleaning liquid supply element 1701 through the cleaning liquid delivery element 1702 into the microporous element 1407, and then the cleaning liquid flows out and is collected by the collecting element 1502. The installation steps and usage methods are the same as those in Example 1.

[0314] The technical effects of this embodiment are as follows:

[0315] By setting up a cleaning unit, the droplets of the micropores of the micropore array chip unit can be cleaned in time by oil washing or the like, thereby reducing the concentration of surfactant molecules and further reducing the exchange of compounds between the droplets.

[0316] Example 5

[0317] This embodiment relates to a high-throughput drug screening system.

[0318] like Fig.13As shown, a high-throughput drug screening system comprises the high-throughput drug screening device 1000, the detection device 2000 and the data processing device 3000 as described in any one of Examples 1 to 4. The detection device 2000 is used to observe and detect the micro-droplets in the microwell array chip unit 1400 to obtain the drug screening result; the data processing device 3000 is connected to the detection device 2000, and is used to receive and analyze the data output by the detection device 2000, and output the analysis result.

[0319] In some of the embodiments, the detection device 2000 includes but is not limited to microscope detection, digital PCR system, and microarray chip analysis platform.

[0320] In some embodiments, the data processing device 3000 includes but is not limited to a computer, a Single on Matrix ® , QuantStudio 3D digital PCR system.

[0321] The technical effects of this embodiment are as follows:

[0322] By setting up detection devices and data processing devices, the nanoliter-scale microdroplets prepared by the high-throughput drug screening device can be detected and data analyzed in a timely manner. The highly integrated and automated devices can be used together to achieve smoother operations and higher efficiency. Researchers can evaluate the effects of drugs more quickly and accurately, thereby promoting the drug development process.

[0323] The above description is only a preferred embodiment of the present invention, and does not limit the implementation mode and protection scope of the present invention. For those skilled in the art, it should be aware that all solutions obtained by equivalent substitutions and obvious changes made using the description and illustrations of the present invention should be included in the protection scope of the present invention.

Claims

1. A high-throughput drug screening device, characterized in that: include: A host unit, the host unit is used to control and provide power to drive the generation and flow of micro-droplets; A dispersed phase liquid supply unit, the dispersed phase liquid supply unit is connected to the main unit and is used to store the dispersed phase liquid and to flow out the dispersed phase liquid under the action of the main unit; A continuous phase liquid supply unit, which is connected to the main unit and is used to store the continuous phase liquid and to discharge the continuous phase liquid under the action of the main unit; A microwell array chip unit, wherein the microwell array chip unit is connected to the dispersed phase liquid supply unit and the continuous phase liquid supply unit, respectively, and is used to form microdroplets from the dispersed phase liquid delivered by the dispersed phase liquid supply unit and the continuous phase liquid delivered by the continuous phase liquid supply unit, and to combine and capture the microdroplets; A collecting unit is connected to the microwell array chip unit and is used to collect liquid flowing out of the microwell array chip unit.

2. The high-throughput drug screening device according to claim 1, characterized in that: The host unit comprises: A host element, the host element is used to provide power to control and drive the formation and flow of micro-droplets; A housing element, wherein the housing element covers the host element and is used to protect the host element; A control element, which is disposed on a side of the housing element and connected to the host element, and is used for a user to control the host element; A display element, which is disposed on the housing element and connected to the host element, and is used to display working parameters of the host element; A first connecting element, which is disposed on a side of the housing element and is respectively connected to the host element and the dispersed phase liquid supply unit; A second connecting element, a plurality of the second connecting elements are arranged on the side of the shell element and are respectively connected to the host element and the continuous phase liquid supply unit.

3. The high-throughput drug screening device according to claim 1, characterized in that: The dispersed phase liquid supply unit comprises: a first clamping element, the first clamping element being disposed on a horizontal plane; A dispersed phase liquid supply element, which is disposed on the first clamping element and is used to store dispersed phase liquid and flow out dispersed phase liquid under the action of the main unit; A dispersed phase transport element, wherein the first end of the dispersed phase transport element is connected to the second end of the dispersed phase liquid supply element, and the second end of the dispersed phase transport element is connected to the microwell array chip unit, and is used to transport dispersed phase liquid into the microwell array chip unit; A first driving element, wherein the first driving element is disposed on the first clamping element, and a second end of the first driving element is connected to a first end of the dispersed phase liquid supply element, and is used to push the dispersed phase inside the dispersed phase liquid supply element to flow out; a third connecting element, the third connecting element being disposed at the first end of the first driving element and connected to the host unit, and being used for transmitting a control signal transmitted by the host unit; a fourth connecting element, which is disposed at the second end of the dispersed phase transport element and is connected to the microwell array chip unit; A first locking element, which is disposed on the first clamping element and is used to lock the first end of the dispersed phase liquid supply element on the first clamping element; and / or The continuous phase liquid supply unit comprises: a second clamping element, the second clamping element being disposed on a horizontal plane; A continuous phase liquid supply element, which is disposed on the second clamping element and is used to store the continuous phase liquid and to flow out the continuous phase liquid under the action of the main unit; A continuous phase transport element, wherein the first end of the continuous phase transport element is connected to the second end of the continuous phase liquid supply element, and the second end of the continuous phase transport element is connected to the microwell array chip unit, and is used to transport the continuous phase liquid into the microwell array chip unit; a second driving element, the second driving element being disposed on the second clamping element, the second end of the second driving element being connected to the first end of the continuous phase liquid supply element, and being used for pushing the continuous phase inside the continuous phase liquid supply element to flow out; a fifth connecting element, the fifth connecting element being disposed at the first end of the second driving element and connected to the host unit, and being used for transmitting a control signal transmitted by the host unit; a sixth connecting element, which is disposed at the second end of the continuous phase transport element and is connected to the microwell array chip unit; A second locking element, wherein the second locking element is disposed on the second clamping element and is used to lock the continuous phase liquid supply element on the second clamping element.

4. The high-throughput drug screening device according to claim 3, characterized in that: The dispersed phase liquid supply unit also includes: a third locking element, the third locking element being disposed at the second end of the first clamping element and locking the second end of the dispersed phase liquid supply element to prevent the second end of the dispersed phase liquid supply element from shaking; and / or The dispersed phase liquid supply unit also includes: a first sliding element, the first sliding element being disposed on a side portion of the first clamping element; a first stabilizing element, the first stabilizing element being connected to the first driving element and being slidably connected to the first sliding element, for stabilizing the position and direction of the first driving element; and or The dispersed phase liquid supply unit also includes: a first buffer element, which is disposed on an inner edge surface of the first locking element and is used to buffer the force between the dispersed phase liquid supply element and the first clamping element; and / or The continuous phase liquid supply unit also includes: a fourth locking element, the fourth locking element being disposed at the second end of the second clamping element and locking the second end of the continuous phase liquid supply element to prevent the second end of the continuous phase liquid supply element from shaking; and / or The continuous phase liquid supply unit also includes: a second sliding element, the second sliding element being disposed on a side portion of the second clamping element; a second stabilizing element, the second stabilizing element being connected to the second driving element and being slidably connected to the second sliding element, for stabilizing the position and direction of the second driving element; and or The continuous phase liquid supply unit also includes: A second buffer element, the second buffer element is arranged on the inner edge surface of the second locking element, and is used to buffer the force between the continuous phase liquid supply element and the second clamping element.

5. The high-throughput drug screening device according to claim 1, characterized in that: The microwell array chip unit comprises: A chip element, wherein the chip element is used to carry micro-droplets; A dispersed phase liquid inlet element, the dispersed phase liquid inlet element is connected to the dispersed phase liquid supply unit and is used to transport the dispersed phase transported by the dispersed phase liquid supply unit; A dispersed phase channel element, wherein a first end of the dispersed phase channel is connected to the dispersed phase liquid inlet element and is used for flowing the dispersed phase transported by the dispersed phase liquid supply unit; A continuous phase liquid inlet element, the continuous phase liquid inlet element is connected to the continuous phase liquid supply unit and is used to transport the continuous phase transported by the continuous phase liquid supply unit; A continuous phase channel element, wherein a first end of the continuous phase channel element is connected to the continuous phase liquid inlet element, and a second end of the continuous phase channel element is connected to the dispersed phase channel element, and is used to form micro-droplets at the second end of the continuous phase channel element; A micro-droplet channel element, wherein the micro-droplet channel element is connected to the second ends of the dispersed phase channel element and the continuous phase channel element respectively, and forms a plurality of transverse and longitudinal branch channels for diverting micro-droplets; A plurality of microporous elements, wherein the plurality of microporous elements are respectively connected to the micro-droplet channel element, and each of the microporous elements is used to accommodate at least one micro-droplet; A plurality of liquid outlet elements are respectively connected to the micro-droplet channel element and the collecting unit, and are used for discharging the micro-droplets flowing out of the micro-droplet channel element.

6. The high-throughput drug screening device according to claim 1, characterized in that: The collecting unit comprises: A conveying element, the conveying element is in communication with the microwell array chip unit and is used to convey the liquid flowing out of the microwell array chip unit; A collecting element is communicated with the conveying element and is used to collect and store liquid flowing out of the conveying element.

7. The high-throughput drug screening device according to any one of claims 1 to 6, characterized in that: Also includes: A fixing unit, which is detachably connected to the microwell array chip unit and is used to fix the microwell array chip unit; and / or A cleaning unit is removably disposed on the dispersed phase liquid supply unit or the continuous phase liquid supply unit and is detachably connected to the micropore array chip unit for cleaning the liquid surface of the micropores of the micropore array chip unit.

8. The high-throughput drug screening device according to claim 7, characterized in that: The fixing unit comprises: A fixing element, wherein the fixing element is hollow and is used to fix the microwell array chip unit and to visualize the microwell array chip unit; A mounting slot element, the mounting slot element is provided on the fixing element and is used to place the microwell array chip unit; A plurality of limiting elements are arranged on the fixing element and are used to limit the position of the microwell array chip unit to prevent the microwell array chip unit from moving.

9. The high-throughput drug screening device according to claim 7, characterized in that: The cleaning unit comprises: A cleaning liquid supply element, which is detachably connected to the dispersed phase supply unit or the continuous phase supply unit and is used to output the cleaning liquid under the action of the host unit; A cleaning liquid delivery element, wherein a first end of the cleaning liquid delivery element is in communication with the cleaning liquid supply element, and a second end of the cleaning liquid delivery element is in communication with the microwell array chip unit, and is used for delivering the cleaning liquid of the cleaning liquid supply element to the microwell array chip unit; A seventh connecting element is disposed at the second end of the cleaning liquid delivery element and is connected to the microwell array chip unit.

10. A high-throughput drug screening system, characterized in that: include The high-throughput drug screening device according to any one of claims 1 to 9; A detection device, which is used to observe and detect microdroplets in the microwell array chip unit to obtain drug screening results; The data processing device is connected to the detection device and is used to receive and analyze the data output by the detection device and output the analysis result.

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