Anti-toppling cell culture bottle

By designing anti-dumping components and automatic sampling system, the problem of cell damage caused by easy dumping of cell culture flasks is solved, and the stability of the cell culture environment and the simplification of the sampling process is achieved.

CN222455045UActive Publication Date: 2025-02-11SHENZHEN RUNKE BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202420097206.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-01-12
Publication Date
2025-02-11
Estimated Expiration
2034-01-12

AI Technical Summary

Technical Problem

During the cell culture process, due to complex operations and complicated personnel, the cell culture flask is easily dumped, causing mechanical damage to the cell and affecting cell morphology and growth.

Method used

An anti-thrust cell culture bottle was designed to fix the bottom plate to the lower surface of the operating table through the back plate, limiting groove, clamp the operating table with the plug and fixing ring. , prevent the culture bottle from pouring. In addition, through the design of the bottle cap, connecting rod and connecting bucket, automatic sampling is achieved when the cover is opened.

Benefits of technology

It effectively prevents the pouring of cell culture flasks, maintains the uniform distribution of culture medium and the stability of the cell growth environment, reduces the risk of cell damage and operation interference, and simplifies the sampling process and improves operation efficiency.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses an anti-toppling cell culture bottle which comprises a main body assembly, a cell culture bottle cover, a cell culture bottle cover and an anti-toppling cell culture bottle cover, and an anti-toppling assembly, the anti-toppling assembly comprises a rear plate fixed to the rear side of the upper surface of the bottom plate, a limiting groove formed in the rear side wall of the outer surface of the rear plate, a clamping plate slidably connected to the interior of the limiting groove, a threaded rod welded to the upper surface of the bottom plate, a fixing ring in threaded connection to the outer surface of the threaded rod, and a first side plate fixed to the left side of the upper surface of the bottom plate. The circular hole is formed in the outer surface of the first side plate; the sampling assembly comprises a bottle cap which is in threaded connection with the outer surface of the bottle opening. The utility model relates to a cell culture bottle, in particular to a cell culture bottle, which aims to solve the problems of cell morphological change, cell membrane rupture and cell death caused by the fact that various operations need to be carried out on an operation table due to complicated operation and various instruments during cell culture, and cells and a culture solution are mechanically damaged when the cell culture bottle is knocked down and poured due to complicated personnel and carelessness.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an anti-dumping cell culture bottle. Background Art

[0002] Cell culture is a commonly used biomedical technology that plays an important role in life research. During the cell culture operation, cell culture flasks are required. Cell culture flasks are a common container used to culture and proliferate cells in a laboratory environment. They are usually made of glass or plastic, have a round bottom or flat bottom design, and come with a lid or stopper to seal the bottle mouth.

[0003] However, it still has the following disadvantages in actual use:

[0004] When culturing cells, due to the complex operation and the numerous instruments, multiple operations need to be performed on the operating table, and there are many people involved. If the cell culture flask is not carefully knocked over, the cells and culture fluid will suffer mechanical damage when the cell culture flask is tipped over, which will cause changes in cell morphology, rupture of cell membranes, and cell death. Utility Model Content

[0005] Technical solutions to be solved

[0006] The utility model aims to provide an anti-tipping cell culture bottle to solve the problems raised in the above-mentioned background technology. When culturing cells, due to the complex operation and various instruments, multiple operations need to be performed on the operating table, and there are many people, the cell culture bottle may be knocked over if not paid attention to. When the cell culture bottle is tipped, the cells and the culture fluid will suffer mechanical damage, which will lead to changes in cell morphology, rupture of cell membranes and cell death.

[0007] Technical Solution

[0008] In order to solve the above technical problems, the utility model is realized by the following technical solutions:

[0009] The utility model discloses an anti-dumping cell culture bottle, comprising:

[0010] A main body component, the main body component comprising a bottle mouth and a bottom plate;

[0011] An anti-dumping assembly, the anti-dumping assembly includes a rear plate fixed to the rear side of the upper surface of the base plate, a limiting groove provided on the rear side wall of the outer surface of the rear plate, a clamping plate slidably connected to the inner part of the limiting groove, a threaded rod welded to the upper surface of the base plate, a fixing ring threadedly connected to the outer surface of the threaded rod, a first side plate fixed to the left side of the upper surface of the base plate, a circular hole provided on the outer surface of the first side plate, a second side plate fixed to the right side of the upper surface of the base plate, a cross bar movably passing through the inner part of the circular hole, a stopper welded to the left side wall of the cross bar, and a clamping column passing through the right side of the cross bar;

[0012] A sampling assembly comprises a bottle cap threadedly connected to the outer surface of the bottle mouth.

[0013] Furthermore, the main body assembly also includes a culture bottle body connected to the lower surface of the bottle mouth;

[0014] Wherein, the bottom plate is fixed on the lower surface of the culture bottle body.

[0015] Furthermore, a circular hole is also formed on the outer surface of the second side plate, and the cross bar passes through the first side plate and the second side plate respectively.

[0016] Furthermore, two circular grooves are provided on the outer surface of the clamping plate, and two threaded rods are provided, respectively passing through the two circular grooves, and the lower surface of the threaded rod is fixed to the upper surface of the bottom plate.

[0017] Furthermore, the fixing ring is threadedly sleeved on the outer surface of the threaded rod, and the clamping plate moves vertically up and down in the limiting groove.

[0018] Furthermore, the sampling assembly also includes a connecting rod clamped on the lower surface of the bottle cap, a connecting bucket fixed on the lower surface of the connecting rod, and a discharge port fixedly connected to the side wall of the outer surface of the connecting bucket.

[0019] Furthermore, the connecting rod extends into the interior of the culture bottle body through the bottle mouth, and the connecting bucket is conical.

[0020] Beneficial Effects

[0021] Compared with the prior art, the advantages of the present invention are:

[0022] 1. The utility model places the bottom plate on the lower surface of the operating table through the rear plate, the limiting groove, the clamping plate, the threaded rod, the fixing ring, the first side plate, the circular hole, the second side plate, the cross bar, the block and the clamping column, and then clamps the clamping plate on the upper surface of the operating table, and then rotates the fixing ring so that the clamping plate and the bottom plate clamp and fix the operating table, then the cross bar is passed through the first side plate and the second side plate, and then the clamping column is passed through the right end of the cross bar to fix the cross bar, so as to prevent the cell culture bottle from tipping over, help to maintain the uniform distribution of the culture solution and the stability of the cell growth environment, ensure that the cells can grow normally, and the operator can perform cell culture operations more easily, reducing unnecessary interference and the risk of damage.

[0023] 2. Based on beneficial effect 1, by setting the bottle cap, connecting rod, connecting bucket and discharge port, when the bottle cap is opened, the connecting rod will be lifted synchronously, and the connecting bucket is inside the culture bottle. When it is lifted, the culture solution in the connecting bucket will be filled. Sampling can be carried out while opening the cap, and there is no need to insert a test tube or other tools directly into the culture bottle. This can simplify the sampling process and improve the convenience and efficiency of operation.

[0024] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0026] Figure 1 It is a schematic diagram of the main components of the utility model;

[0027] Figure 2 This is a structural diagram of the anti-dumping assembly of the utility model;

[0028] Figure 3 It is a schematic diagram of the sampling component of the utility model.

[0029] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0030] 11. Culture bottle body; 12. Bottle mouth; 13. Bottom plate; 21. Back plate; 211. Limiting groove; 22. Clamping plate; 23. Threaded rod; 24. Fixing ring; 25. First side plate; 251. Circular hole; 26. Second side plate; 27. Cross bar; 28. Block; 29. ​​Clamping column; 31. Bottle cap; 32. Connecting rod; 33. Connecting bucket; 34. Discharge outlet. DETAILED DESCRIPTION

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below with reference to the accompanying drawings.

[0032] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific implementation methods disclosed below.

[0033] In order to make the purpose, technical solution and advantages of the present invention more clear, the implementation mode of the present invention will be further described in detail below with reference to the accompanying drawings.

[0034] Embodiment 1

[0035] See also Figure 1-Figure 2 As shown, this embodiment is a tip-proof cell culture bottle, comprising:

[0036] The main body component includes a bottle mouth 12, a bottom plate 13, and a culture bottle body 11 connected to the lower surface of the bottle mouth 12;

[0037] Wherein, the bottom plate 13 is fixed on the lower surface of the culture bottle body 11;

[0038] The bottom plate 13 is mounted on the culture bottle body 11, and the bottle mouth 12 is used for adding or sampling;

[0039] The anti-dumping assembly includes a rear plate 21 fixed to the rear side of the upper surface of the bottom plate 13, a limiting groove 211 provided on the rear side wall of the outer surface of the rear plate 21, a clamping plate 22 slidably connected to the inner part of the limiting groove 211, a threaded rod 23 welded to the upper surface of the bottom plate 13, a fixing ring 24 threadedly connected to the outer surface of the threaded rod 23, a first side plate 25 fixed to the left side of the upper surface of the bottom plate 13, a circular hole 251 provided on the outer surface of the first side plate 25, a second side plate 26 fixed to the right side of the upper surface of the bottom plate 13, a cross bar 27 movably passing through the inner part of the circular hole 251, a stopper 28 welded to the left side wall of the cross bar 27, and a clamping column 29 passing through the right side of the cross bar 27;

[0040] The rear plate 21 is installed on the clamping plate 22, the threaded rod 23 limits the clamping plate 22, and the fixing ring 24 fixes the clamping plate 22 at a desired position, and the rear plate 21 is at the rear side of the culture bottle body 11 to prevent the culture bottle from tipping backwards;

[0041] The outer surface of the second side plate 26 is also provided with a circular hole 251, and the cross bar 27 passes through the first side plate 25 and the second side plate 26 respectively;

[0042] The first side plate 25 and the second side plate 26 are located on the left and right sides of the culture bottle body 11 to prevent the culture bottle from tipping over.

[0043] The outer surface of the clamping plate 22 is provided with two circular grooves, and two threaded rods 23 are provided, respectively passing through the two circular grooves, and the lower surface of the threaded rod 23 is fixed to the upper surface of the bottom plate 13;

[0044] The cross bar 27 is passed through the first side plate 25 and the second side plate 26, and the clamping column 29 is passed through the right side of the cross bar 27 to fix the cross bar 27;

[0045] Working principle:

[0046] First, the staff puts the back plate 21 against the edge of the operating table, puts the bottom plate 13 on the lower surface of the operating table, and fits the lower surface of the clamping plate 22 with the upper surface of the operating table;

[0047] Secondly, the fixing ring 24 is rotated again, and the fixing ring 24 drives the clamping plate 22 to move downward, and the clamping plate 22 and the bottom plate 13 clamp the operating table tightly;

[0048] Next, the cross bar 27 is passed through the first side plate 25 and the second side plate 26, and the stopper 28 is clamped on the left side of the circular hole 251, and then the clamping column 29 is passed through the right end of the cross bar 27;

[0049] Thus, the culture bottle body 11 is placed on the upper surface of the bottom plate 13, and the culture bottle is prevented from tipping over by the rear plate 21, the first side plate 25, the second side plate 26 and the cross bar 27;

[0050] This step can prevent the cell culture flask from tipping over, help maintain the uniform distribution of the culture solution and the stability of the cell growth environment, ensure that the cells can grow normally, and operators can perform cell culture operations more easily, reducing unnecessary interference and the risk of damage.

[0051] Embodiment 2

[0052] See also Figure 3 As shown, this embodiment is based on the above embodiment 1 and also includes:

[0053] The sampling assembly includes a bottle cap 31 threadedly connected to the outer surface of the bottle mouth 12, a connecting rod 32 clamped on the lower surface of the bottle cap 31, a connecting bucket 33 fixed on the lower surface of the connecting rod 32, and a discharge port 34 fixedly connected to the side wall of the outer surface of the connecting bucket 33;

[0054] The bottle cap 31 receives the connecting rod 32, and the bottle cap 31 corresponds to the bottle mouth 12 in a positive direction, and the bottle cap 31 is threadedly connected to the outer surface of the bottle mouth 12;

[0055] The connecting rod 32 passes through the bottle mouth 12 and extends into the interior of the culture bottle body 11, and the connecting bucket 33 is conical;

[0056] The connecting rod 32 is installed to the connecting bucket 33, and the interior of the connecting bucket 33 is empty, and the discharge port 34 prevents the culture solution from dripping and causing waste when pouring the culture solution;

[0057] Working principle:

[0058] First, when you need to sample the liquid inside the culture bottle;

[0059] At this time, the staff only needs to rotate and unscrew the bottle cap 31, and the bottle cap 31 will drive the connecting rod 32 to rotate. After unscrewing the bottle cap 31, lift the bottle cap 31 upward, and the connecting rod 32 will be simultaneously driven to lift upward;

[0060] Next, the connecting rod 32 is lifted, and the connecting bucket 33 at the lower end is driven to move upward, and the connecting bucket 33 is filled with culture fluid, and the culture fluid is taken out through the discharge port 34 for testing;

[0061] In this step, sampling can be performed while opening the cover, without inserting a test tube or other tools directly into the culture bottle, which can simplify the sampling process and improve the convenience and efficiency of the operation.

[0062] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0063] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein with equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A tip-proof cell culture bottle, characterized in that: include: A main body component, the main body component comprising a bottle mouth (12) and a bottom plate (13); An anti-tipping component, the anti-tipping component comprising a rear plate (21) fixed to the rear side of the upper surface of the base plate (13), a limiting groove (211) provided on the rear side wall of the outer surface of the rear plate (21), a clamping plate (22) slidably connected to the inside of the limiting groove (211), a threaded rod (23) welded to the upper surface of the base plate (13), a fixing ring (24) threadedly connected to the outer surface of the threaded rod (23), a first side plate (25) fixed to the left side of the upper surface of the base plate (13), a circular hole (251) provided on the outer surface of the first side plate (25), a second side plate (26) fixed to the right side of the upper surface of the base plate (13), a cross bar (27) movably passing through the inside of the circular hole (251), a stopper (28) welded to the left side wall of the cross bar (27), and a clamping column (29) passing through the right side of the cross bar (27); A sampling assembly, comprising a bottle cap (31) threadedly connected to the outer surface of a bottle mouth (12).

2. The anti-dumping cell culture bottle according to claim 1, characterized in that: The main body assembly also includes a culture bottle body (11) connected to the lower surface of the bottle mouth (12); Wherein, the bottom plate (13) is fixed on the lower surface of the culture bottle body (11).

3. The anti-dumping cell culture bottle according to claim 1, characterized in that: The outer surface of the second side plate (26) is also provided with a circular hole (251), and the cross bar (27) passes through the first side plate (25) and the second side plate (26) respectively.

4. The anti-dumping cell culture bottle according to claim 1, characterized in that: The outer surface of the clamping plate (22) is provided with two circular grooves, and two threaded rods (23) are provided, respectively passing through the two circular grooves, and the lower surface of the threaded rod (23) is fixed to the upper surface of the bottom plate (13).

5. The anti-dumping cell culture bottle according to claim 1, characterized in that: The fixing ring (24) is threadedly sleeved on the outer surface of the threaded rod (23), and the clamping plate (22) moves vertically up and down in the limiting groove (211).

6. The anti-dumping cell culture bottle according to claim 1, characterized in that: The sampling assembly also includes a connecting rod (32) clamped on the lower surface of the bottle cap (31), a connecting bucket (33) fixed on the lower surface of the connecting rod (32), and a discharge port (34) fixedly connected to the side wall of the outer surface of the connecting bucket (33).

7. The anti-dumping cell culture bottle according to claim 6, characterized in that: The connecting rod (32) passes through the bottle mouth (12) and extends into the interior of the culture bottle body (11), and the connecting bucket (33) is conical.