96-well plate capable of preventing cross contamination

Abstract

The utility model discloses a 96-well plate capable of preventing cross contamination, which belongs to the field of 96-well plates and comprises a well plate and a well plate cover covering the well plate, ninety-six hole grooves distributed in a rectangular array are arranged on the inner side of the well plate, and the well plate cover covers the well plate. The hole plate cover comprises a transparent cover plate, a cover cap which is clamped on the transparent cover plate and is in contact with an opening in the top end of the hole groove, and a fence which sleeves the outer part of the hole plate and is clamped with the bottom of the transparent cover plate. According to the 96-well plate capable of preventing cross contamination, cross contamination of reagents is effectively prevented through the design of the well plate cover and the cap on the well plate cover, the hole grooves distributed in the well plate in a rectangular array mode are matched with the well plate cover capable of being connected in a covering mode, the internal situation can be observed through the transparent cover plate, and leakage of the reagents in each hole groove is avoided through the sealing design of the cap; the enclosure and the buckle structure ensure the firm fixation of the pore plate cover, and further enhance the closeness of the system.

Description

Technical Field

[0001] The utility model relates to the field of 96-well plates, in particular to a 96-well plate capable of preventing cross contamination. Background Art

[0002] The 96-well plate is a multifunctional experimental tool. The design of the 96-well plate enables the experimenter to process multiple samples at the same time, greatly improving the experimental efficiency. The product features of this deep-well plate are mainly reflected in the following aspects: high throughput, the 96-well design allows the experimenter to process up to 96 samples at the same time; easy to operate, the design of the deep-well plate makes the addition and sampling operations simple and fast; good sealing, the deep-well plate is usually made of high-quality materials, which can effectively prevent the volatilization and cross-contamination of samples; high temperature and high pressure resistance, this deep-well plate can withstand high temperature and high pressure environments, suitable for experiments such as PCR amplification that require high temperature and high pressure conditions; reusable, reducing experimental costs.

[0003] A Chinese utility model patent with publication number CN219129305U discloses a 96-well plate, which belongs to the technical field of medical devices. The 96-well plate is formed by adding a slidingly connected cover plate on the basis of the original well plate, and the cover plate has a through hole aligned with the well plate and two baffles that can be separated from each other on the through hole, and then the two baffles are triggered to separate by a moving device moving on the cover plate, so that the through hole on the moving device is connected to the culture well of the well plate through the through hole of the cover plate, and the confirmation step is increased by adding an unlocking operation to avoid operator misoperation. However, due to the stacked cover plates and moving plates, the well plate itself is prone to have a hole position far away from the dropper, and sputtering is prone to occur when dripping, resulting in reagents remaining on the moving plate. When the contaminated moving plate unlocks other well positions and drips liquid, cross contamination is prone to occur, resulting in inaccurate experimental results, so the practicality is poor.

[0004] Therefore, a 96-well plate that prevents cross contamination is provided to solve the above problems. Utility Model Content

[0005] 1. Technical issues to be resolved

[0006] The utility model provides a 96-well plate for preventing cross contamination, aiming to solve the problem that the existing 96-well plate mentioned in the background technology is prone to cross contamination.

[0007] (II) Technical solution

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solution: a 96-well plate for preventing cross contamination, comprising a well plate and a well plate cover mounted on the well plate.

[0009] In order to prevent cross-contamination of reagents, the inner side of the well plate is provided with ninety-six wells distributed in a rectangular array, and the well plate cover includes a transparent cover plate, a cap snapped on the transparent cover plate and in contact with the top opening of the well slot, and an enclosure mounted on the outside of the well plate and snapped on the bottom of the transparent cover plate, and the enclosure is provided with a buckle for locking the enclosure and the well plate; by covering the well plate cover on the well plate, the internal situation can be observed through the transparent cover plate of the well plate cover, and the caps arranged at the position corresponding to each well slot on the transparent cover plate are covered on the well slot port, thereby preventing the reagent inside the well slot from being exposed, thereby avoiding cross-contamination of the reagents in the well slot, and the enclosure cooperates with the buckle to be locked on the well plate, which can not only make the well plate cover firmly stuck on the well plate, but also further avoid the connection between the inside of the well plate and the outside, and prevent external contaminants from entering.

[0010] In order to facilitate targeted operations, the cap includes a cap barrel covering the opening at the top end of the hole slot, a cap cover hinged on the cap and buckled with the cap, and a skirt band mounted on the outside of the top end of the hole slot and fixedly connected to the cap barrel; by arranging an openable cap cover on the cap, it is convenient to select the hole slot for taking and placing reagents, which is more convenient and quick, while ensuring that other hole slots are always closed to avoid cross contamination. At the same time, while the cap barrel presses the hole slot port, a skirt band is also provided to wrap around the end of the hole slot to further prevent loosening and ensure the sealing effect of the hole slot.

[0011] Preferably, in order to facilitate maintenance and replacement of the cap, a cover hole corresponding to the hole groove is opened on the transparent cover plate, and an annular groove is opened at the position of the outer wall of the cap tube corresponding to the cover hole; the cap tube is clamped on the cover hole of the transparent cover plate through the annular groove, which has a simple structure and is easy to disassemble and assemble, thereby making maintenance and replacement more convenient.

[0012] Preferably, in order to facilitate the cleaning of the transparent cover and the enclosure, the bottom of the transparent cover is provided with plug-in blocks at the positions corresponding to the four corners, and the enclosure is provided with card slots at the positions corresponding to the plug-in blocks; the transparent cover and the enclosure are connected by the card blocks and the card slots, so that the transparent cover and the enclosure can be disassembled, which is convenient for disassembly and cleaning on the one hand, and can also reduce maintenance costs on the other hand.

[0013] Preferably, in order to achieve locking of the orifice plate and the orifice plate cover, a card block is provided at a position on the orifice plate corresponding to the buckle; by arranging the card block on the orifice plate, after the enclosure is stuck on the orifice plate, the card block can be automatically engaged with the buckle to lock it, and when disassembling, the buckle can be unlocked by pushing it outward, and the structure is simple, stable, and easy to operate.

[0014] Preferably, in order to facilitate opening of the cap, the side of the cap is provided with a brim; by providing the brim on the cap, the brim can be pressed when opening the cap, providing a landing point for the fingers, thereby making it easier to apply force using the lever principle.

[0015] Preferably, in order to facilitate observation and positioning of the hole slots and avoid selection errors, a letter sequence aligned with the horizontal row of the hole slots is provided on one side of the top of the hole plate corresponding to the vertical row of the hole slots, and a number sequence aligned with the vertical row of the hole slots is provided on one side of the top of the hole plate corresponding to the horizontal row of the hole slots; by marking the letter sequence and the number sequence on one side of the horizontal and vertical rows of the hole slots respectively, a reference can be provided during observation and selection, and positioning can be performed using the intersection of the letter sequence and the number sequence.

[0016] Preferably, in order to prevent light from affecting the reagent inside the well slot, a shading mechanism is provided on one side of the well plate cover, and the shading mechanism includes a storage box clamped on the side wall of the enclosure by a positioning slider, a winding shaft rotatably connected to the inside of the storage box, a shading cloth fixed to and wound on the winding shaft at one end, and a torsion spring sleeved on the outside of the winding shaft and with two ends respectively connected to the winding shaft and the inner wall of the storage box, wherein the end of the shading cloth away from the winding shaft passes through the storage box and has a torsion spring that abuts against the outer wall of the storage box. The end frame is connected, and the end frame has a hanging buckle for hanging with the transparent cover plate; the external light can be avoided by the shading mechanism. Specifically, the end frame can be pulled to drive the shading cloth to be unwound from the winding shaft and pulled out from the storage box until the surface of the orifice plate cover is completely covered, and then the hanging buckle is used to hang it on the other end of the transparent cover plate to completely cover the transparent cover plate. After unlocking the hanging buckle, the shading cloth will be automatically rewound into the storage box by the winding shaft under the action of the torsion spring. The operation is simple and quick, and the storage box can also be disassembled and assembled from the enclosure by the positioning slider, which is more practical.

[0017] Preferably, in order to facilitate the hanging of the buckle on the transparent cover, the end of the transparent cover away from the storage box has a buckle seat horizontally aligned with the buckle; by setting the buckle seat on the transparent cover, the buckle can be directly hung on the buckle seat, which has a simple structure and is convenient and quick to operate.

[0018] (III) Beneficial effects

[0019] The 96-well plate for preventing cross-contamination effectively prevents cross-contamination of reagents through the well plate cover and the cap design thereon. The well slots distributed in a rectangular array inside the well plate cooperate with the well plate cover that can be covered, so that not only the internal situation can be observed through the transparent cover, but also the leakage of reagents in each well slot can be avoided through the sealing design of the cap. The enclosure and buckle structure ensure the firm fixation of the well plate cover, further enhancing the closedness of the system.

[0020] The 96-well plate that prevents cross contamination has an openable cap that allows the user to take and place reagents in a targeted manner, which is easy to operate and does not affect the closure of other wells.

[0021] The 96-well plate that prevents cross contamination also provides an easy-to-operate light-shielding mechanism that can effectively avoid the influence of light on reagents. The entire system not only improves the accuracy and safety of experimental operations, but also reduces maintenance costs. It is an ideal choice for reagent management and use in laboratories. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic diagram of the structure of a 96-well plate for preventing cross contamination;

[0023] Figure 2 A schematic diagram of the explosion structure of a 96-well plate to prevent cross contamination;

[0024] Figure 3 A schematic diagram of the overall structure of a cap in a 96-well plate to prevent cross contamination;

[0025] Figure 4 for Figure 2 The enlarged structural diagram at A in the middle;

[0026] Figure 5 A schematic diagram of the internal structure of a storage box in a 96-well plate to prevent cross contamination.

[0027] In the figure:

[0028] 1. Orifice plate; 11. Hole slot; 12. Card block; 13. Letter sequence; 14. Number sequence;

[0029] 2. hole plate cover; 21. transparent cover plate; 211. cover hole; 212. plug block; 213. buckle seat;

[0030] 22, cap; 221, hat tube; 2211, ring groove; 2212, skirt; 222, cap; 2221, brim; 23, enclosure; 231, buckle;

[0031] 3. Shading mechanism; 31. Storage box; 311. Positioning slider; 32. Winding shaft; 33. Shading cloth; 331. End frame; 3311. Hanging buckle; 34. Torsion spring. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] Example 1

[0034] See also Figure 1-Figure 4The utility model provides a technical solution: a 96-well plate for preventing cross contamination, comprising a well plate 1 and a well plate cover 2 mounted on the well plate 1, wherein the inner side of the well plate 1 has ninety-six well slots 11 distributed in a rectangular array, the well plate cover 2 comprises a transparent cover plate 21, a cap 22 clamped on the transparent cover plate 21 and in contact with the top opening of the well slot 11, and a baffle 23 sleeved on the outside of the well plate 1 and clamped on the bottom of the transparent cover plate 21, wherein the baffle 23 has a buckle 231 for locking the baffle 23 and the well plate 1.

[0035] The cap 22 includes a cap barrel 221 that covers the top opening of the hole slot 11 , a cap cover 222 that is hinged on the cap 22 and buckled with the cap 22 , and a skirt 2212 that is sleeved on the outside of the top of the hole slot 11 and fixedly connected to the cap barrel 221 .

[0036] When in use, since the orifice plate cover 2 is covered on the orifice plate 1, the internal situation can be observed through the transparent cover plate 21 of the orifice plate cover 2, and the cover cap 22 set at the position corresponding to each orifice slot 11 on the transparent cover plate 21 is covered on the port of the orifice slot 11, thereby preventing the internal reagent of the orifice slot 11 from being exposed, thereby avoiding cross contamination of the reagent in the orifice slot 11, and the enclosure 23 is locked on the orifice plate 1 with the buckle 231, which can not only make the orifice plate cover 2 firmly stuck on the orifice plate 1, but also further avoid the connection between the inside of the orifice plate 1 and the outside world, and prevent external pollutants from entering. Since an openable cap 222 is set on the cap 22, it is convenient to select the orifice slot 11 for taking and placing reagents, which is more convenient and quick, and at the same time ensures that other orifice slots 11 are always closed to avoid cross contamination. At the same time, while the cap tube 221 presses the port of the orifice slot 11, a skirt 2212 is also provided to wrap around the end of the orifice slot 11 to further prevent loosening and ensure the sealing effect of the orifice slot 11.

[0037] Furthermore, a cover hole 211 corresponding to the hole groove 11 is formed on the transparent cover plate 21, and an annular groove 2211 is formed on the outer wall of the cap barrel 221 at a position corresponding to the cover hole 211; the cap barrel 221 is clamped on the cover hole 211 of the transparent cover plate 21 through the annular groove 2211, which has a simple structure and is easy to disassemble and assemble, thereby making maintenance and replacement more convenient.

[0038] In addition, there are plug blocks 212 at the positions corresponding to the four corners of the bottom of the transparent cover 21, and there are card slots at the positions corresponding to the plug blocks 212 on the enclosure 23; the transparent cover 21 and the enclosure 23 are connected by the card blocks 12 and the card slots, so that the transparent cover 21 and the enclosure 23 can be disassembled, which is convenient for disassembly and cleaning on the one hand, and can also reduce maintenance costs on the other hand.

[0039] Specifically, a block 12 is provided at a position on the orifice plate 1 corresponding to the buckle 231. By setting the block 12 on the orifice plate 1, after the enclosure 23 is stuck on the orifice plate 1, the buckle 231 can be used to automatically buckle the block 12 to lock it. When disassembling, the buckle 231 can be pushed outward to unlock it. The structure is simple, stable, and easy to operate.

[0040] Furthermore, the side of the cap 222 is provided with a brim 2221; by providing the brim 2221 on the cap 222, when opening the cap 222, the brim 2221 can be pressed to provide a landing point for fingers, thereby making it easier to apply force using the lever principle.

[0041] It should be supplemented that a letter sequence 13 aligned with the horizontal row of the hole slots 11 is provided on one side of the top of the hole plate 1 corresponding to the vertical row of the hole slots 11, and a number sequence 14 aligned with the vertical row of the hole slots 11 is provided on one side of the top of the hole plate 1 corresponding to the horizontal row of the hole slots 11; by marking the letter sequence 13 and the number sequence 14 on one side of the horizontal row and the vertical row of the hole slots 11 respectively, a reference can be provided during observation and selection, and positioning can be performed using the intersection of the letter sequence 13 and the number sequence 14.

[0042] Example 2

[0043] Different from Example 1, Figure 1 , Figure 2 and Figure 5 As shown, a shading mechanism 3 is provided on one side of the orifice cover 2, and the shading mechanism 3 includes a storage box 31 clamped on the side wall of the enclosure 23 through a positioning slider 311, a winding shaft 32 rotatably connected to the inside of the storage box 31, a shading cloth 33 fixed at one end to and wound around the winding shaft 32, and a torsion spring 34 which is sleeved on the outside of the winding shaft 32 and whose two ends are respectively connected to the winding shaft 32 and the inner wall of the storage box 31, wherein the end of the shading cloth 33 away from the winding shaft 32 passes through the storage box 31 and has an end frame 331 abutting against the outer wall of the storage box 31, and the end frame 331 has a hook 3311 hooked to the transparent cover plate 21, wherein the enclosure 23 is opened A positioning slot corresponding to the positioning slider 311 is provided; the shading mechanism 3 can avoid external light. Specifically, pulling the end frame 331 can drive the shading cloth 33 to be unwound from the winding shaft 32 and pulled out from the storage box 31 until it completely covers the surface of the orifice cover 2, and then use the hook 3311 to hang it on the other end of the transparent cover 21 to completely cover the transparent cover 21. After unlocking the hook 3311, the shading cloth 33 will be automatically rewound into the storage box 31 by the winding shaft 32 under the action of the torsion spring 34. The operation is simple and quick, and the storage box 31 can also be disassembled and assembled from the positioning slot of the enclosure 23 through the positioning slider 311, which is more practical.

[0044] Among them, the end of the transparent cover 21 away from the storage box 31 has a hook seat 213 horizontally aligned with the hook 3311; through the hook seat 213 set on the transparent cover 21, the hook 3311 can be directly hung on the hook seat 213, which has a simple structure and is convenient and quick to operate.

[0045] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A 96-well plate for preventing cross contamination, comprising a well plate (1) and a well plate cover (2) mounted on the well plate (1), characterized in that: The inner side of the orifice plate (1) has ninety-six orifice slots (11) distributed in a rectangular array, the orifice plate cover (2) comprises a transparent cover plate (21), a cover cap (22) clamped on the transparent cover plate (21) and in contact with the top opening of the orifice slots (11), and a fence (23) sleeved on the outside of the orifice plate (1) and clamped on the bottom of the transparent cover plate (21), the fence (23) having a buckle (231) for locking the fence (23) and the orifice plate (1); The cover cap (22) comprises a cap barrel (221) covering the top opening of the hole slot (11), a cap cover (222) hinged on the cover cap (22) and buckled with the cover cap (22), and a skirt (2212) sleeved on the outside of the top of the hole slot (11) and fixedly connected to the cap barrel (221).

2. A 96-well plate for preventing cross contamination according to claim 1, characterized in that: The transparent cover plate (21) is provided with a cover hole (211) corresponding to the hole groove (11), and the outer wall of the cap tube (221) is provided with an annular groove (2211) at a position corresponding to the cover hole (211).

3. A 96-well plate for preventing cross contamination according to claim 2, characterized in that: The bottom of the transparent cover plate (21) is provided with insert blocks (212) at positions corresponding to the four corners, and the enclosure (23) is provided with card slots at positions corresponding to the insert blocks (212).

4. A 96-well plate for preventing cross contamination according to claim 3, characterized in that: A clamping block (12) is provided on the orifice plate (1) at a position corresponding to the clamping buckle (231).

5. A 96-well plate for preventing cross contamination according to claim 4, characterized in that: The side of the cap (222) is provided with a brim (2221).

6. A 96-well plate for preventing cross contamination according to claim 5, characterized in that: A letter sequence (13) aligned with the horizontal row of the hole slots (11) is provided on one side of the top of the orifice plate (1) corresponding to the vertical row of the hole slots (11), and a number sequence (14) aligned with the vertical row of the hole slots (11) is provided on one side of the top of the orifice plate (1) corresponding to the horizontal row of the hole slots (11).

7. A 96-well plate for preventing cross contamination according to claim 6, characterized in that: A shading mechanism (3) is provided on one side of the orifice plate cover (2), and the shading mechanism (3) comprises a storage box (31) clamped on the side wall of the enclosure (23) through a positioning slider (311), a winding shaft (32) rotatably connected to the inside of the storage box (31), a shading cloth (33) with one end fixed to the winding shaft (32) and wound around the winding shaft (32), and a torsion spring (34) sleeved on the outside of the winding shaft (32) and with two ends respectively connected to the winding shaft (32) and the inner wall of the storage box (31), wherein the end of the shading cloth (33) away from the winding shaft (32) passes through the storage box (31) and has an end frame (331) abutting against the outer wall of the storage box (31), and the end frame (331) has a hook (3311) hung with the transparent cover plate (21).

8. A 96-well plate for preventing cross contamination according to claim 7, characterized in that: One end of the transparent cover plate (21) away from the storage box (31) has a hook seat (213) horizontally aligned with the hook (3311).

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