Three-dimensional co-culture organoid chip
By designing three-dimensional co-culture organoid chips, including micropore culture units and culturers, the problem that existing chips are difficult to meet the needs of diversified experimental results is solved, and cost reduction and support for multiple co-culture systems are achieved.
Patent Information
- Application Number
- CN202421184425.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-28
- Publication Date
- 2025-02-14
- Estimated Expiration
- 2034-05-28
AI Technical Summary
The existing organoid culture chips are integrated into a structure, which is difficult to meet the diverse experimental needs, resulting in increased research costs.
A three-dimensional co-culture organoid chip is designed, including a micropore culture unit and a culturer. The microculture holes of the micropore culture unit are hemispherical or composed of cylindrical holes and hemispherical holes, which can simulate a low-matter exchange environment and support Co-culture of organoids and other cells.
It has achieved diversified experimental needs, reduced research costs, and can obtain organoids with good consistency through micropore cultures of consistent sizes, and supports the establishment of multiple co-culture systems.
Smart Images
Figure CN222476626U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biological culture, and specifically to an organoid culture device. Background Art
[0002] Organoids are derived from the body's own tissues or stem cells. They are three-dimensional structural models with the characteristics of original tissues and organs formed through in vitro culture. They are highly similar to the source tissues in terms of tissue structure, cell type and function. Compared with traditional two-dimensional cell line models and animal models, organoid models have a composition and behavior that is closer to physiological cells, a more stable genome and simpler operations. They have significant advantages in terms of cycle, cost, clinical relevance and high-throughput screening. Based on this, organoids have great potential in the field of tumor research.
[0003] At present, organoid culture can be mainly divided into two categories: with culture scaffolds and without culture scaffolds. The former provides physical support, and cells grow on it to form organoid structures, such as hydrogel embedding culture; the latter forces cells to aggregate into cell spheres and grow into organoids during the culture process through forced floating method, hanging drop method and stirring method. At present, organoid culture chips are increasingly used due to their advantages such as simple operation and easy standardization compared with other methods. However, the current organoid culture chips are integrated structures, which are difficult to meet the diverse experimental needs, and small-batch customization of chips according to experimental design will inevitably lead to increased research costs. Utility Model Content
[0004] In view of this, the purpose of the present invention is to provide a three-dimensional co-culture organoid chip to solve the technical problem that the existing organoid culture chip is an integrated structure and is difficult to meet various experimental needs.
[0005] The utility model discloses a three-dimensional co-culture organoid chip, which comprises a microporous culture unit, wherein the microporous culture unit comprises a bottom plate and a surrounding edge formed on the edge of the bottom plate, and micro-culture holes are evenly distributed on the bottom plate; the micro-culture holes are hemispherical holes, or the micro-culture holes are composed of vertical cylindrical holes and hemispherical holes connected to the lower ends of the cylindrical holes.
[0006] Furthermore, the diameter of the micro-culture well is 0.3-1 mm.
[0007] Furthermore, the height of the cylindrical hole in the micro-culture well is 0-7.5 mm.
[0008] Furthermore, it also includes a culture vessel, on which culture holes for placing microporous culture units are arranged.
[0009] Furthermore, there are at least two culture holes on the culture device, and the culture holes are connected by a channel, and the position of the channel is higher than the microporous culture unit.
[0010] Beneficial effects of the utility model:
[0011] 1. In the three-dimensional co-culture organoid chip of the utility model, the bottom of the micro-culture well of the micro-well culture unit is hemispherical. After the cells are inoculated, the cells are deposited at the bottom of the micro-culture well due to gravity, and a three-dimensional structure is formed under the action of mechanical force.
[0012] 2. In the three-dimensional co-culture organoid chip of the utility model, when the depth of the micro-culture well is deepened to a certain extent, the material exchange at the bottom of the well is reduced, thereby simulating a low material exchange environment.
[0013] 3. The three-dimensional co-culture organoid chip of the utility model can obtain organoids with good consistency through microwell culture of uniform size.
[0014] 4. The three-dimensional co-culture organoid chip of the present invention, when used in combination with a microporous culture unit and a culture vessel, can achieve co-culture of organoids and other cells; by controlling the liquid level of the culture medium, a variety of co-culture systems can be achieved.
[0015] 5. The microporous culture unit of the three-dimensional co-culture organoid chip of the present invention can also be used in combination with a general cell culture well plate or culture dish to achieve simple co-culture or organoid expansion culture.
[0016] 6. The microporous culture unit of the three-dimensional co-culture organoid chip in the present invention can be used alone or in combination with a culture vessel with channels between culture wells; the microporous culture unit can also be used in combination with an ordinary cell culture dish or an ordinary cell culture well plate; the microporous culture unit can be used flexibly and in a variety of ways to better meet the needs of different experimental scenarios.
[0017] 7. The production cost of the three-dimensional co-culture organoid chip in the utility model is low. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a three-dimensional schematic diagram of a circular microwell culture unit.
[0019] Figure 2 It is a three-dimensional schematic diagram of a square microwell culture unit.
[0020] Figure 3 The cross-sectional view of the microwell culture unit in which the microwell is hemispherical.
[0021] Figure 4 The cross-sectional view of a microwell culture unit in which the microwell is composed of a cylindrical hole and a hemispherical hole.
[0022] Figure 5 It is a schematic diagram of the combination of a microporous culture unit and a culture vessel;
[0023] Figure 6 Schematic diagram of the combination of a microporous culture unit and a common culture dish. DETAILED DESCRIPTION
[0024] The utility model is further described below in conjunction with the accompanying drawings and embodiments.
[0025] Embodiment 1: Figure 1-4 As shown, the three-dimensional co-culture organoid chip in this embodiment includes a microporous culture unit, which includes a bottom plate 1 and a surrounding edge 2 formed on the edge of the bottom plate, and micro-culture wells 3 are evenly distributed on the bottom plate. Figure 3 The micro-culture well is a hemispherical well; or Figure 4 As shown, the micro-culture well is composed of a vertical cylindrical hole and a hemispherical hole connected to the lower end of the cylindrical hole. The diameter of the micro-culture well is 0.3-1 mm. The height of the cylindrical hole in the micro-culture well is 0-7.5 mm. The shape of the microporous culture unit can be circular, square, or can be designed into other shapes as needed.
[0026] In the three-dimensional co-culture organoid chip of this embodiment, the bottom of the micro-culture well of the micro-culture unit is hemispherical. After the cells are inoculated, the cells are deposited at the bottom of the micro-culture well due to gravity, and a three-dimensional structure is formed under the action of mechanical force.
[0027] In the three-dimensional co-culture organoid chip of this embodiment, when the depth of the microculture well is deepened to a certain extent, the material exchange at the bottom of the well is reduced, thereby simulating a low material exchange environment.
[0028] The three-dimensional co-culture organoid chip in this embodiment can obtain organoids with good consistency through microwell culture of uniform size.
[0029] Embodiment 2: In this embodiment, the three-dimensional co-culture organoid chip includes the microporous culture unit described in Embodiment 1, and also includes a culture vessel 4, on which a culture well 5 for placing the microporous culture unit is provided. There are at least two culture wells on the culture vessel, and the culture wells are connected by a channel 6, and the position of the channel is higher than the microporous culture unit.
[0030] When used in combination, the microporous culture unit and the incubator can achieve co-culture of organoids. The following is an example of the combination of the microporous culture unit and the incubator:
[0031] Place the microporous culture unit on the bottom of the culture well on the incubator to form an annular space between the microporous culture unit and the wall of the culture well. Inoculate tumor microenvironment-related cells in the annular space. The cell suspension should not cover the organoid culture unit. Place it in a 37°C incubator for 2 hours before taking it out. Inoculate the cell suspension of tumor cells in the microporous culture unit. Do not overflow the culture unit. Place it in a 37°C incubator for 30 minutes before taking it out. Carefully aspirate the liquid outside the microwells on the microporous culture unit, and then slowly add culture medium to the annular space until it covers the microporous culture unit but not higher than the channel on the incubator. Culture in a 37°C incubator, changing the liquid every 3 days until organoids are formed in the microwells.
[0032] On the basis of the above-mentioned combination, culture medium can also be added to make the culture medium higher than the channel on the culture device, so that the culture media between the culture wells are interconnected to form a complex co-culture system.
[0033] like Figure 6 As shown, the microporous culture units can also be densely placed on a common culture vessel 7 for co-culture of organoids. Inoculate cells in the microporous culture unit without overflowing the culture unit, and place in a 37°C incubator for 30 minutes before taking out. Carefully aspirate the liquid outside the micro-wells on the microporous culture unit, add culture medium to cover the microporous culture unit, place in a 37°C incubator, and change the liquid every 3 days to obtain organoids through mass culture.
[0034] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.
Claims
1. A three-dimensional co-culture organoid chip, characterized in that: It comprises a microporous culture unit, which comprises a bottom plate and a surrounding edge formed on the edge of the bottom plate, and micro-culture holes are evenly distributed on the bottom plate; the micro-culture holes are hemispherical holes, or the micro-culture holes are composed of vertical cylindrical holes and hemispherical holes connected to the lower ends of the cylindrical holes.
2. The three-dimensional co-culture organoid chip according to claim 1, characterized in that: The diameter of the micro-culture well is 0.3-1 mm.
3. The three-dimensional co-culture organoid chip according to claim 2, characterized in that: The height of the cylindrical hole in the microculture well is 0-7.5 mm.
4. The three-dimensional co-culture organoid chip according to any one of claims 1 to 3, characterized in that: The invention also comprises a culture vessel, wherein the culture vessel is provided with culture holes for placing the microporous culture units.
5. The three-dimensional co-culture organoid chip according to claim 4, characterized in that: There are at least two culture holes on the culture device, and the culture holes are connected by a channel, and the position of the channel is higher than the microporous culture unit.