Fumigation treatment device for batch soil microbial biomass measurement

Abstract

The fumigation treatment device comprises a tank body, a tank cover and a vacuum pump, an opening is formed in the top of the tank body, the opening is opened or closed through the tank cover, the tank cover is installed on the tank body through a clamping structure, and the vacuum pump is installed on the tank body. The vacuum pump is used for sucking air in the tank body, the clamping structure is characterized in that the clamping structure comprises a rotating piece installed on the top of the tank body and a clamping piece installed on the tank cover, the rotating piece comprises a screw rod and a nut, one end of the screw rod is hinged to the top of the tank body, and the other opposite end of the screw rod is connected with the nut; the installation position of the clamping piece corresponds to the position of the rotating piece, and the clamping piece is of a structure with a longitudinal opening, so that when the screw is turned upwards, a rod body of the screw is clamped into the opening of the clamping piece, and the nut installed on the screw is located on the upper side of the opening. The device has the advantages of safe and simple operation, high efficiency, less pollution and the like.

Description

Technical Field

[0001] The utility model relates to the technical field of soil microbial biomass determination, in particular to a fumigation treatment device for batch soil microbial biomass determination. Background Art

[0002] Soil microorganisms are the driving force for the transformation and circulation of soil organic matter and soil nutrients. They participate in the decomposition of organic matter, the formation of humus, and the transformation and circulation of nutrients. Soil microbial biomass is a reservoir of soil nutrients and an important source of nutrients available for plant growth. It can reflect the actual content and potential of microorganisms in the soil and has become one of the hot spots in soil science research at home and abroad. Soil microbial biomass includes microbial carbon, microbial nitrogen, microbial phosphorus and microbial sulfur. They can all be determined by chloroform fumigation-extraction method. There are some problems with the commonly used fumigation devices at present, such as the glass drying dish needs to be coated with glue to achieve the sealing effect, and it is very easy to leak due to poor sealing if the operation is not careful; the glass drying dish has limited tolerance, and there is a safety hazard of glass breakage and injury during the vacuuming process; the sealant is sticky and easy to contaminate the vessel and sample; the glass drying dish is placed according to the routine operation, the sample amount of one fumigation is limited, and the detection efficiency is low; after the fumigation, each sample needs to be taken out one by one, which increases the chance of the tester to contact chloroform, which is not good for the health of the personnel.

[0003] Current data have proposed improvements to fumigation devices, but they are mainly in the areas of turning, stirring, oscillating and constant temperature cultivation of soil samples. Turning or vibrating samples during fumigation may cause sample spillage and increase detection errors. Especially for sticky and heavy samples, turning the sample over makes it difficult to disperse the particles, but may make the particles stick more tightly, affecting the fumigation effect. The above-mentioned device adds auxiliary facilities and has a complex structure, which is not conducive to the fumigation of batch samples. The study of soil microbial biomass requires a large amount of samples to be tested and analyzed, and the sample types are complex. It is necessary to have a device that is simple to operate and can fully fumigate batches of soil. At present, there are few devices suitable for fumigation of batch samples and reducing personnel exposure to harmful reagents, which are difficult to meet related research needs.

[0004] For example, the existing patent technology CN 217133163 U discloses a multi-layer drying dish for carbon-nitrogen fumigation of soil microbial biomass, which solves the technical problem of low fumigation efficiency. However, the holes opened in each layer of the patent technology cannot form a stable flow channel for chloroform, and the chloroform moves irregularly in each layer, which is not conducive to the full fumigation of each sample. It takes a long time to ensure that the sample is fully fumigated. In addition, the patent technology has a large one-time fumigation volume, and the amount of chloroform also increases accordingly, thereby increasing the risk of contact between the operator and chloroform when taking out the sample.

[0005] The disclosure of the above background technology content is only used to assist in understanding the concept and technical solution of the utility model. It does not necessarily belong to the prior art of this patent application. In the absence of clear evidence that the above content has been disclosed on the filing date of this patent application, the above background technology should not be used to evaluate the novelty and creativity of the present application. Utility Model Content

[0006] The utility model (mainly) aims to provide a fumigation treatment device for batch samples, which replaces glass drying dishes, does not need sealants, is equipped with multi-layer sample racks, can fully fumigate hundreds of samples at a time, is safe and simple to operate, is efficient, and has little pollution. At the same time, by placing samples in layers in an inner barrel, the contact time between personnel and chloroform is reduced, which is beneficial to protecting the health of operators.

[0007] To this end, the utility model provides a fumigation treatment device for batch soil microbial biomass determination.

[0008] Preferably, the utility model may also have the following technical features:

[0009] A fumigation treatment device for batch soil microbial biomass determination comprises a tank body, a tank cover, and a vacuum pump. The top of the tank body is open, and the opening is opened or closed by the tank cover. The tank cover is installed on the tank body through a snap-fit ​​structure. The vacuum pump is used to suck the air inside the tank body. It is characterized in that: the snap-fit ​​structure comprises a rotating part installed on the top of the tank body and a snap-fit ​​part installed on the tank cover, wherein the rotating part comprises a screw and a nut, one end of the screw is hinged on the top of the tank body, and the other opposite end is connected to the nut; the installation position of the snap-fit ​​part corresponds to the position of the rotating part, and it is a structure with a longitudinal opening, so that when the screw is flipped upward, the rod body of the screw is snapped into the opening of the snap-fit ​​part, and the nut installed on the screw is on the upper side of the opening.

[0010] Furthermore, 3 to 6 groups of snap-fit ​​structures are provided to connect the tank body and the tank cover.

[0011] Furthermore, it also includes a sealing ring, which is installed on the top surface of the tank body to strengthen the seal between the tank body and the tank cover to ensure the stability of the vacuum degree inside the tank body.

[0012] Furthermore, the fumigation treatment device also includes an inner barrel, which is installed inside the tank body and has an outer diameter smaller than the inner diameter of the tank body; an inner barrel gasket supporting the inner barrel is provided at the lower part of the tank body, so that the inner barrel and the bottom of the tank body form a discharge space for placing containers; the bottom of the inner barrel is also perforated, and a plurality of layers of inner barrel partitions are arranged at intervals on the inner wall; wherein the inner barrel partition includes a middle hole partition and a side hole partition, and the middle hole partition is a circular plate with an air hole in the middle; the side hole partition is a circular plate structure, and a plurality of air holes are evenly arranged around it; the side hole partition and the middle hole partition are sequentially arranged in a circular manner inside the inner barrel.

[0013] Furthermore, the upper end surface of the inner barrel 4 is flush with the upper end surface of the tank body 1 or lower than the upper end surface of the tank body 1 .

[0014] Furthermore, the inner barrel wall is provided with a plurality of partition gaskets of different heights for supporting the inner barrel partition, and the partition gaskets are horizontally arranged annular rings and are arranged along the circumferential direction of the inner barrel wall.

[0015] Furthermore, the inner barrel gasket is a horizontally arranged annular ring and is arranged along the circumferential direction of the inner wall of the tank body, so that the surrounding of the lower side of the inner barrel is pressed on the upper side of the inner barrel gasket.

[0016] Furthermore, a pressure gauge is installed on the tank cover.

[0017] A fumigation treatment device for batch soil microbial biomass determination comprises a tank body, a tank cover, and a vacuum pump. The top of the tank body is open, and the opening is opened or closed by the tank cover. The vacuum pump is used to suck the air inside the tank body. The fumigation treatment device also comprises an inner barrel, which is installed inside the tank body and has an outer diameter smaller than the inner diameter of the tank body. The lower part of the tank body is provided with an inner barrel gasket for supporting the inner barrel, so that the inner barrel and the bottom of the tank body form a discharge space for placing containers. The bottom of the inner barrel is also provided with perforations, and a plurality of layers of inner barrel partitions are arranged at intervals on the inner wall. The inner barrel partition comprises a middle hole partition and a side hole partition, and the middle hole partition is a circular plate with air holes in the middle. The side hole partition is a circular plate structure, and a plurality of air holes are evenly opened around it. The side hole partition and the middle hole partition are sequentially cyclically arranged inside the inner barrel.

[0018] Compared with the prior art, the beneficial effects of the utility model include: by setting an inner barrel, the sample is placed on the partition of the inner barrel for fumigation, and when the fumigation is finished, the inner barrel can be quickly taken out to take out all the samples, reducing the time that personnel are in contact with chloroform. The pores specially set on each layer of the partition of this device create a flow path for the chloroform vapor, so that the chloroform vapor can contact each layer of samples to achieve the effect of full fumigation. Because the samples can be placed in multiple layers, the amount of fumigation can reach hundreds at a time, which is several times the amount of fumigation samples of conventional drying dishes. The detection efficiency is high and can meet the needs of batch sample detection and analysis. The can lid of this device is provided with a silicone ring, which is sealed with the can body in the form of a screw. This method avoids the use of sticky sealant. The sealing operation of the screw is simple and hygienic, the sealing effect is good, and it is not easy to leak, which is conducive to full fumigation. The device proposed by this patent is simple in design, easy to operate, and highly practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the tank structure of the utility model.

[0020] Figure 2 It is a schematic diagram of the tank cover structure of the utility model.

[0021] Figure 3 It is a schematic diagram of the structure of the utility model, and the snap-fitting structure is not shown.

[0022] Figure 4 It is a schematic diagram of the inner barrel structure of the utility model.

[0023] Figure 5 It is a schematic diagram of the side hole partition of the utility model.

[0024] Figure 6 It is a schematic diagram of the middle hole partition of the utility model. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below in conjunction with specific implementations and in conjunction with the accompanying drawings. It should be emphasized that the following description is merely exemplary and is not intended to limit the scope and application of the present invention.

[0026] Non-limiting and non-exclusive embodiments will be described with reference to the following figures, wherein like reference numerals refer to like parts unless otherwise specifically stated.

[0027] Example 1

[0028] like Figures 1 to 6The fumigation treatment device for batch soil microbial biomass determination shown in the figure comprises a tank body 1, a tank cover 2, and a vacuum pump 13. The top of the tank body 1 is open, and the opening is opened or closed by the tank cover 2. The vacuum pump 13 is used to suck the air inside the tank body 1. As one of the improvements, the tank cover 2 is installed on the tank body 1 through a snap-fit ​​structure. Figure 2-3 The clamping structure includes a rotating member installed on the top of the tank body and a clamping member installed on the tank cover, wherein the rotating member includes a screw 113 and a nut 112, one end of the screw 113 is hinged on the top of the tank body 1, and the other end is connected to the nut 112; the position of the clamping member corresponds to the position of the rotating member, and it is a structure with a longitudinal opening, so that when the screw 113 is turned upward, the rod of the screw 113 is clamped into the opening 121 of the clamping member, and the nut 112 installed on the screw 113 is on the upper side of the opening 121, so that by adjusting the position of the nut 112, the tank body 1 and the tank cover 2 are tightly connected to achieve the effect of sealing the tank body 1. 3 to 6 groups of clamping structures are set to connect the tank body 1 and the tank cover 2, so that the joint surfaces of the tank body 1 and the tank cover 2 are fully fitted and tightly to prevent the chloroform vapor from escaping. Preferably, a sealing ring is also included, which is a silicone sealing ring and is installed on the top surface of the tank body 1 to strengthen the seal between the tank body 1 and the tank cover 2 to ensure that the vacuum inside the tank body 1 is stable for a long time.

[0029] The purpose is to reduce the operator's contact time and amount with chloroform vapor to ensure the operator's health and safety. Figure 3 The fumigation treatment device also includes an inner barrel 4, which is installed inside the tank body 1, and its outer diameter is smaller than the inner diameter of the tank body 1. The lower part of the tank body 1 is provided with an inner barrel gasket 9 for supporting the inner barrel 4, so that the inner barrel 4 and the bottom of the tank body 1 form a discharge space for placing a sodium hydroxide solution container 8, an aqueous solution container 10, and a chloroform container 11. The upper end surface of the inner barrel 4 is flush with the upper end surface of the tank body 1 or lower than the upper end surface of the tank body 1. The bottom of the inner barrel 4 is provided with an axial through hole, and the inner wall is provided with a plurality of layers of inner barrel partitions. Among them, referring to Figure 5-6The inner barrel partition includes a middle hole partition 5 and a side hole partition 6. The middle hole partition 5 is a circular plate with air holes 51 in the middle; the side hole partition 6 is a circular plate structure with a plurality of air holes 61 evenly opened around it. During installation, the side hole partition 6 and the middle hole partition 5 are sequentially placed in the inner barrel 4 in a circular manner, and the side hole partition 6 is installed in the lowest layer, that is, among the several layers of inner barrel partitions installed in the inner barrel 4, the side hole partition 6 is at the lowest layer, the middle hole partition 5 is in the upper layer, and the side hole partition 6 is installed in the upper layer. In this way, the side hole partition 6 and the middle hole partition 5 are placed in a circular manner, so that the chloroform vapor forms a specific flow route, which can efficiently and fully fumigate the sample placed on the inner barrel partition. Preferably, a pressure gauge 3 is installed on the tank cover 2 to observe the vacuum degree inside the tank body 1.

[0030] Combination Figure 4 , a plurality of partition gaskets 7 of different heights are fixed to the inner wall of the inner barrel 4, and the partition gaskets 7 are horizontally arranged annular rings and arranged along the circumferential direction of the inner wall of the inner barrel 4. When in use, the lower side of the inner barrel partition is naturally pressed on the upper side of the partition gasket 7, and the inner barrel partition is pressed against the partition gasket 7 by the dead weight of the inner barrel partition and the weight of the sample, thereby preventing chloroform vapor from entering the upper side of the corresponding partition gasket 7 from the contact surface between the inner barrel partition and the partition gasket 7, and disrupting the movement path of the chloroform vapor.

[0031] In the example shown in the attached drawings of the specification, after the vacuum pump 13 is turned on, when the vacuum degree in the tank body 1 reaches a certain level, the chloroform vapor first diffuses from the perforations 41 at the bottom of the inner barrel 4 to the space at the bottom of the inner barrel (the space between the bottom inner barrel partition and the bottom of the inner barrel), and then diffuses over the sample placed at the bottom of the inner barrel in the process of diffusing to the surroundings, and then fumigates the sample; the chloroform vapor continues to move, and continues to diffuse upward through the peripheral pores 61 of the bottom inner barrel partition (side hole partition 6), and after diffusing over the sample placed on the side hole partition, it continues to diffuse upward through the central pores 51 of the higher inner barrel partition (middle hole partition 5). Since the chloroform vapor is continuously generated, a special path is set for the flow and dispersion of the chloroform vapor according to the pores of each layer of the inner barrel partition, so that the samples on each layer of the inner barrel partition can fully contact with the chloroform vapor, and achieve full fumigation, which is conducive to the accurate determination of soil microbial biomass. The special path refers to the sequential circular installation of the middle hole partition plate 5 and the side hole partition plate 6, so that the chloroform vapor moves from the middle to both sides, then from both sides to the middle, and then from both sides to the middle according to the position change of the pores of the middle hole partition plate 5 and the side hole partition plate 4. Compared with the perforated partition plate scheme in the existing patent technology CN 217133163U, the pores between the inner barrel partition plates of each layer of the present application are more conducive to the movement of chloroform vapor, and can also form a more stable movement path, so that chloroform can fully contact the sample during the movement. When placing the sample in the inner barrel, avoid the perforations at the bottom of the inner barrel and the pores on the inner barrel partition plate to ensure smooth passage of chloroform vapor. During fumigation, the chloroform container is directly below the perforations at the bottom of the inner barrel, and the spacing between adjacent inner barrel partition plates is the height of the sample container plus 1 to 3 cm, and the diameter of the sample container is smaller than the diameter of the pores. That is, after the sample container is placed on the inner barrel partition, the distance between the upper end surface of the sample container and the upper inner barrel partition is 1 to 3 cm.

[0032] Preferably, the inner barrel gasket 9 is a horizontally arranged annular ring and is arranged along the circumferential direction of the inner wall of the tank body 1, so that the surrounding of the lower side of the inner barrel 4 is pressed on the upper side of the inner barrel gasket 7. When in use, the contact surface between the inner barrel 4 and the inner barrel gasket 9 is pressed tightly by the dead weight of the inner barrel 4 and the weight of the sample to have a certain sealing performance.

[0033] The method for using the fumigation treatment device for batch soil microbial biomass determination comprises the following steps:

[0034] Step S1, placing the sodium hydroxide container 8, the aqueous solution container 10, and the chloroform container 11 in the discharge space at the bottom of the tank body 1, and the chloroform container 11 is located directly below the perforation at the bottom of the inner barrel 4, which is conducive to the passage of chloroform vapor through the perforation.

[0035] Step S2, placing the inner barrel 4 in the tank body 1, and placing the sample containers at the bottom of the inner barrel 4. After the sample containers are placed at the bottom of the inner barrel 4, inner barrel partitions are laid layer by layer in the inner barrel according to the number of samples and the sample containers are placed;

[0036] In step S2, the inner barrel partitions laid layer by layer are the side hole partitions 6 and the middle hole partitions 5 arranged in a cycle, so that the upper and lower layers of the side hole partitions 6 are both middle hole partitions 5. At the same time, the bottom of the inner barrel is regarded as the middle hole partition 5. Therefore, the side hole partition 6 is used as the first inner barrel partition laid in the inner barrel 1.

[0037] Step S3, after the sample is discharged, the tank cover 2 is pressed tightly against the tank body 1, and the vacuum pump is turned on to extract the air inside the tank body 1, so that the chloroform in the chloroform container boils to generate steam to fumigate the sample;

[0038] Step S4, after the fumigation is completed, the inner barrel 1 is quickly taken out to transfer the fumigated sample, and the sample and the inner barrel are transferred together, which has high transfer efficiency and can also reduce the contact time between personnel and chloroform, thereby ensuring the health and safety of personnel.

[0039] Example 2

[0040] The focus of this embodiment is on the structure of the inner barrel and its role in the fumigation treatment device. Figure 3-6 A fumigation treatment device for batch soil microbial biomass determination shown in the figure comprises a tank body 1, a tank cover 2, and a vacuum pump 13. The top of the tank body 1 is open, and the opening is opened or closed by the tank cover 2. The vacuum pump 13 is used to suck the air inside the tank body 1. The fumigation treatment device also includes an inner barrel 4, which is installed inside the tank body 1 and has an outer diameter smaller than the inner diameter of the tank body 1. The lower part of the tank body 1 is provided with an inner barrel gasket supporting the inner barrel 4, so that the inner barrel 4 and the bottom of the tank body 1 form a discharge space for placing containers; the bottom of the inner barrel 4 is also provided with a perforation 41, and a plurality of layers of inner barrel partitions are arranged at intervals on the inner wall; wherein the inner barrel partition comprises a middle hole partition 5 and a side hole partition 6, the middle hole partition 5 is a circular plate with air holes 51 in the middle; the side hole partition 6 is a circular plate structure, and a plurality of air holes 61 are evenly opened around it; the side hole partition 61 and the middle hole partition 51 are sequentially arranged in the inner barrel 4 in a circular manner. Among them, the fumigation treatment device including the inner barrel has been specifically introduced in the above-mentioned embodiment 1, and will not be repeated here.

[0041] Those skilled in the art will appreciate that numerous variations to the above description are possible, and that the examples and figures are intended only to describe one or more specific implementations.

[0042] Although exemplary embodiments of the present invention have been described and described, it will be understood by those skilled in the art that various changes and substitutions may be made thereto without departing from the spirit of the present invention. In addition, many modifications may be made to adapt specific situations to the teachings of the present invention without departing from the central concept of the present invention described herein. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but the present invention may also include all embodiments and their equivalents that fall within the scope of the present invention.

Claims

1. A fumigation treatment device for batch soil microbial biomass determination, comprising a tank body, a tank cover, and a vacuum pump, wherein the top of the tank body is open and the opening is opened or closed by the tank cover, the tank cover is mounted on the tank body by a snap-fit ​​structure, and the vacuum pump is used to suck the air inside the tank body, characterized in that: The snap-fit ​​structure includes a rotating part installed on the top of the tank body and a snap-fit ​​part installed on the tank cover, wherein the rotating part includes a screw and a nut, one end of the screw is hinged on the top of the tank body, and the other opposite end is connected to the nut; the installation position of the snap-fit ​​part corresponds to the position of the rotating part, and it is a structure with a longitudinal opening, so that when the screw is flipped upward, the rod body of the screw is stuck in the opening of the snap-fit ​​part, and the nut installed on the screw is on the upper side of the opening.

2. The fumigation treatment device for batch soil microbial biomass determination according to claim 1, characterized in that: 3 to 6 groups of snap-fit ​​structures are arranged to connect the tank body and the tank cover.

3. The fumigation treatment device for batch soil microbial biomass determination according to claim 1, characterized in that: It also includes a sealing ring, which is installed on the top surface of the tank body to strengthen the seal between the tank body and the tank cover to ensure the stability of the vacuum degree inside the tank body.

4. The fumigation treatment device for batch soil microbial biomass determination according to claim 1, characterized in that: The fumigation treatment device also includes an inner barrel, which is installed inside the tank body and has an outer diameter smaller than the inner diameter of the tank body; an inner barrel gasket supporting the inner barrel is provided at the lower part of the tank body, so that the inner barrel and the bottom of the tank body form a discharge space for placing containers; the bottom of the inner barrel is also provided with perforations, and a plurality of layers of inner barrel partitions are arranged at intervals on the inner wall; wherein the inner barrel partitions include a middle hole partition and a side hole partition, and the middle hole partition is a circular plate with air holes in the middle; the side hole partition is a circular plate structure, and a plurality of air holes are evenly opened around it; the side hole partition and the middle hole partition are sequentially cyclically arranged inside the inner barrel.

5. The fumigation treatment device for batch soil microbial biomass determination according to claim 4, characterized in that: The upper end surface of the inner barrel is flush with the upper end surface of the tank body or lower than the upper end surface of the tank body.

6. The fumigation treatment device for batch soil microbial biomass determination according to claim 4, characterized in that: The inner barrel wall is provided with a plurality of partition gaskets of different heights for supporting the inner barrel partition, and the partition gaskets are horizontally arranged annular rings and are arranged along the circumferential direction of the inner barrel wall.

7. The fumigation treatment device for batch soil microbial biomass determination according to claim 4, characterized in that: The inner barrel gasket is a horizontally arranged annular ring and is arranged along the circumferential direction of the inner wall of the tank body so that the surrounding of the lower side of the inner barrel is pressed on the upper side of the inner barrel gasket.

8. The fumigation treatment device for batch soil microbial biomass determination according to claim 1, characterized in that: A pressure gauge is installed on the tank cover.

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