Biological reader
By combining fluorescence monitoring and image acquisition technology in biological readers, analyzing the image and fluorescence data of microbial indicators, the problem of fluorescence change detection in the prior art is solved, and higher monitoring accuracy is achieved. reliability.
Patent Information
- Application Number
- CN202420248852.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-02-01
- Publication Date
- 2025-02-14
- Estimated Expiration
- 2034-02-01
AI Technical Summary
Existing biological readers are susceptible to environmental factors in fluorescence change detection, which leads to the accuracy of the judgment results that cannot reach expectations, and false negative and false positive phenomena often occur; at the same time, the color changes of biological indicators can only be observed through the naked eye and cannot be Implement screen visualization.
Design a biological reader, combining fluorescence monitoring and image acquisition, analyze the image and fluorescence data of microbial indicators through the main control board, monitor and determine the sterilization situation in real time, and avoid the appearance of false positives and false negatives.
By recording and analyzing real-time images and fluorescence changes of microbial indicators, the accuracy and reliability of monitoring are improved, and the occurrence of false positives and false negatives is effectively avoided, and the reliability of real-time monitoring is enhanced.
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Figure CN222482109U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of microbial detection, in particular to a biological reader. Background Art
[0002] The biological reader uses thermophilic Bacillus stearothermophilus spores and Bacillus subtilis spores, and uses their metabolites in the process of growth and reproduction to react with indicators of specific components. The reaction products can produce a fluorescent effect under ultraviolet irradiation, and use a photosensitive receiving sensor for fluorescence detection. According to the intensity of the detected fluorescence, the concentration of specific metabolites can be quickly determined, so as to know the survival of the spores and finally monitor the sterilization situation. The biological reader is mainly used for sterilization and disinfection of hospital equipment and instruments and detection of sterilization-unqualified items.
[0003] Existing biological readers include, for example, Chinese utility model patent authorization announcement CN210465440U, which discloses a biological reader for verifying the sterilization effect, including a base 1 and an outer shell 2 arranged on the base 1, the outer shell 2 is provided with a display screen 3, a verification mechanism 4 is located in the outer shell 2, a reagent tube 5 with an indicator inside is inserted into the verification mechanism 4, the verification mechanism 4 includes a reaction tube 41 with a biological indicator inside, a photoelectric module 42 and a light sensor 43, the photoelectric module 42 emits ultraviolet light, the ultraviolet light is irradiated to the reaction tube 41 so that fluorescence is generated in the reaction tube 41, and then the light sensor 43 is used to perform fluorescence detection, and the concentration of a specific metabolite is quickly judged according to the intensity of the detected fluorescence.
[0004] Another example is the Chinese utility model patent authorization announcement CN207908511U, which discloses a biological reader, including a shell, a heating component, a detection mechanism and a main control mechanism 150, the shell is provided with a channel groove 116 for placing a reagent tube; the heating component is used to provide a suitable temperature for the growth of microorganisms in the reagent tube; the detection mechanism includes a mechanical microswitch and a detection switch control board, the mechanical microswitch is electrically connected to the detection switch control board, the mechanical microswitch is used to detect whether a reagent tube is placed in the channel groove 116, and output the detection information to the detection switch control board; the main control mechanism is electrically connected to the detection switch control board, and the main control mechanism is electrically connected to the heating component; the main control mechanism is used to control whether the heating component is heated according to the detection information.
[0005] For example, Chinese utility model patent application publication CN106841040A discloses a microorganism detection system 100, including a microorganism culture solution placement device 120 for placing microorganism culture solution; a micro-switch detection device 122 is provided in the microorganism culture solution placement device 120, which is used to detect whether there is a reagent tube 150 containing microorganism culture solution in the microorganism culture solution placement device 120; a light source device 131 is used to emit ultraviolet rays that produce a fluorescence effect with the microorganism culture solution; a fluorescence receiving device 132 is used to receive the fluorescence generated by the fluorescence effect and output the microorganism concentration data corresponding to the fluorescence; a control device 140 is used to receive and analyze the microorganism metabolite concentration data, wherein the microorganism culture solution placement device 120, the light source device 131 and the fluorescence receiving device 132 are all electrically connected to the control device 140.
[0006] The above-mentioned prior art has the following disadvantages: (1) Since fluorescence changes are easily affected by environmental factors such as temperature, the disinfection results can only be identified by the fluorescence change value, and the accuracy of the judgment results cannot reach the expected goal, and false negatives and false positives often occur; (2) The color change of the biological indicator can only be observed by the naked eye, and it is impossible to achieve screen visualization. Utility Model Content
[0007] In view of the technical problems existing in the prior art, the utility model aims to provide a biological reader, which can monitor the fluorescence changes of microbial indicators and the color changes of biological monitoring, so as to avoid the occurrence of false positives and false negatives, and further increase the reliability of real-time monitoring.
[0008] According to one aspect of the utility model, a biological reader is provided, comprising a housing, a display module (2), a microorganism indicator test tube (4), an image collector (8), an ultraviolet lamp (16), a fluorescence monitoring template (15) and a main control board (17); the main control board (17) is arranged inside the housing and below the display module (2);
[0009] The shell comprises an upper shell (3) and a lower shell (18); the display module (2) is fixed to the upper shell (3) via a display module fixing member (1); the microorganism indicator test tube (4) is placed on the upper shell (3) via a channel groove; the image collector (8), the ultraviolet lamp (16), the fluorescence monitoring template (15) and the main control board (17) are arranged in the shell; the image collector (8) is used to record the image of the microorganism indicator in the microorganism indicator test tube (4) and transmit the image to the main control board (17); the ultraviolet lamp (16) is used to emit ultraviolet rays that produce a fluorescence effect with the microorganism indicator; the fluorescence monitoring template (15) is used to receive fluorescence generated by the fluorescence effect and output microorganism concentration data corresponding to the fluorescence to the main control board (17); the main control board (17) determines the sterilization condition based on the received image of the microorganism indicator and the microorganism concentration data.
[0010] Preferably, a first monitoring plate (7) and a second monitoring plate (14) are arranged inside the shell, and the first monitoring plate (7) and the second monitoring plate (14) are respectively located on both sides of the microbial indicator test tube (4).
[0011] The image collector (8) is arranged on the first monitoring board (7);
[0012] The ultraviolet lamp (16) and the fluorescent monitoring template (15) are arranged on the second monitoring board (14).
[0013] Preferably, a heat insulating member (13) is provided on one side of the second monitoring plate (14).
[0014] Preferably, it also includes a dust cover arranged on the upper shell body to prevent dust from entering the microbial indicator test tube.
[0015] Preferably, it also includes a mechanical switch arranged on the first monitoring board, which is used to determine whether a microbial indicator test tube is inserted into the channel slot.
[0016] Preferably, a light-transmitting plate and a status light plate are also provided at the front end of the upper shell for judging the working status of the biological reader.
[0017] Preferably, it also includes a heating body, on which a heating plate is provided for heating the microbial indicator test tube.
[0018] Preferably, a heat insulating member is provided on one side of the second monitoring plate.
[0019] Compared with the prior art, the utility model has the following beneficial effects:
[0020] The real-time image of the microbial indicator is recorded while recording the fluorescence changes, and each frame of the recorded image is transmitted to the chip on the main control board for calculation and analysis, which increases the reliability of real-time monitoring and effectively avoids the occurrence of false positives and false negatives. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 The present invention is a three-dimensional appearance diagram of a biological reader of the present invention.
[0022] Figure 2 The utility model is a side view of the internal structure of a biological reader.
[0023] Figure markings: 1-display module fixing member; 2-display module; 3-upper shell; 4-microorganism indicator test tube; 5-dust cover; 6-mechanical switch; 7-first monitoring board; 8-image collector; 9-status light board; 10-light-transmitting board; 11-heating body; 12-heating plate; 13-insulating member; 14-second monitoring board; 15-fluorescent monitoring template; 16-ultraviolet lamp; 17-main control board; 18-lower shell. DETAILED DESCRIPTION
[0024] The embodiments of the present invention are described below in conjunction with the accompanying drawings. Those skilled in the art should understand that these embodiments are only illustrative and not restrictive.
[0025] Figure 1 The present invention is a three-dimensional external light diagram of a biological reader. Figure 2 It is a side view of the internal structure of a biological reader of the utility model.
[0026] like Figure 1 and Figure 2 As shown, the utility model provides a biological reader, including a housing, a display module 2, a microorganism indicator test tube 4, an image collector 8, an ultraviolet lamp 16, a fluorescence monitoring template 15 and a main control board 17; the housing is divided into an upper housing 3 and a lower housing 18, and the display module 2 is fixed on the upper housing 3 by a display module fixing member 1, wherein the display module 2 can be a display, such as a liquid crystal display LCD, and the display module 2 can be tilted relative to the upper housing 3, and is at a certain angle to the upper housing 3, and is supported and fixed by the display module fixing member 1. The main control board 17 is arranged inside the housing and below the display module 2. The main control board 17 has a control chip to realize the control of the working state of the entire biological reader.
[0027] The housing 3 is provided with a plurality of channel slots (not shown), and the microbial indicator test tube 4 is placed on the upper housing 3 through the channel slots (not shown). The image collector 8, the ultraviolet lamp 16, the fluorescence monitoring template 15 and the main control board 17 are arranged in the housing. The image collector 8 is used to record the image of the microbial indicator in the microbial indicator test tube 4 and transmit the image to the main control board 17; the image collector 8 can be a camera. Since the image collector 8 is used to shoot the microbial indicator, the image collector 8 can use a biological camera, a photographic microscope and other equipment. Microbial indicators are a special type of live microbial products that can be used to confirm the performance of sterilization equipment, verify the sterilization procedure, monitor the sterilization effect of the production process, etc.
[0028] like Figure 2 As shown, a first monitoring plate 7 and a second monitoring plate 14 are arranged inside the housing. The first monitoring plate 7 and the second monitoring plate 14 are respectively located on both sides of the microbial indicator test tube 4. Figure 2 In the embodiment, the first monitoring plate 7 is located on the left side of the microbial indicator test tube 4, that is, on the side close to the front end of the biological reader, and the image collector 8 can be arranged on the first monitoring plate 7 and at a lower position to realize the acquisition and recording of the image of the microbial indicator in the microbial indicator test tube 4. The second monitoring plate 14 is located on the other side of the microbial indicator test tube 4, and the first monitoring plate 7 and the second monitoring plate 14 are arranged vertically relative to the lower shell 18.
[0029] The ultraviolet lamp 16 and the fluorescence monitoring template 15 are arranged on the second monitoring board 14. The ultraviolet lamp 16 is used to emit ultraviolet rays that produce a fluorescence effect with the microbial indicator. The fluorescence monitoring template 15 is used to receive the fluorescence generated by the fluorescence effect and output the microbial concentration data corresponding to the fluorescence to the main control board 17; the main control board 17 determines the sterilization situation based on the received image of the microbial indicator and the microbial concentration data.
[0030] The image recording module 8 disposed on the first monitoring board 7 and the fluorescent monitoring template 15 disposed on the second monitoring board 14 are disposed opposite to each other on both sides of the microbial indicator test tube 4, and are close in height relative to the microbial indicator test tube 4. The ultraviolet lamp 16 is located above the fluorescent monitoring template 15 and is at a certain angle to the second monitoring board 14, so as to emit ultraviolet light to the middle and lower part of the microbial indicator test tube 4 to produce a fluorescent effect with the microbial indicator.
[0031] When microbial monitoring is required, the image collector 8 is turned on while the fluorescence monitoring is being performed, and the color change trend of the microbial indicator is finally presented on the display module 2 in the form of a report through the processing operation of the chip of the main control board 17 .
[0032] Alternatively, if Figure 2As shown, the biological reader further includes a dust cover 5 disposed on the upper housing 3 to prevent dust from entering the microbial indicator test tube 4. The dust cover 5 is detachably disposed relative to the upper housing 3, and the dust cover 5 can be a transparent plate.
[0033] Optionally, the biological reader further includes a mechanical switch 6 disposed on the first monitoring board 7, for determining whether the microorganism indicator test tube 4 is inserted into the channel slot. The mechanical switch 6 can be located near the upper portion of the first monitoring board 7 and at a certain distance from the image collector 8 to avoid affecting the image recording work of the image collector 8.
[0034] Optionally, the front end of the upper housing 3 is also provided with a light-transmitting plate 10 and a status light board 9 for judging the working state of the biological reader. The status light board 9 can be connected to and controlled by the main control board. When the biological reader is turned on, the status light board 9 is turned on and lights up for indication. When the biological reader is not in working state, the status light board 9 is off.
[0035] Optionally, the biological reader further comprises a heating body 11 , which is located below the microorganism indicator test tube 4 , and a heating plate 12 is provided on the heating body 11 for heating the microorganism indicator test tube 4 .
[0036] Optionally, a heat insulating member 13 is provided on one side of the second monitoring plate 14. Figure 2 The heat insulating member 13 is disposed at the bottom of the second monitoring plate 14 and is located on the side where the heating body 11 is located to isolate the heat generated by the heating body 11 .
[0037] The working process of the biological reader provided by the utility model can be as follows: S1, insert the microbial indicator test tube 4 into any channel slot of the housing 3; S2, the ultraviolet lamp 16 emits ultraviolet light that produces a fluorescent effect with the microbial indicator, and the fluorescent monitoring template 15 receives the fluorescence generated by the fluorescent effect, and outputs the microbial concentration data corresponding to the fluorescence to the main control board 17; S3, the image collector 8 records the image of the microbial indicator in the microbial indicator test tube 4, and transmits the image to the main control board 17;
[0038] S4. The main control board 14 determines the sterilization status based on the received image of the microorganism indicator and the microorganism concentration data.
[0039] Optionally, in step S3, the image collector 8 uploads image information every 5 minutes.
[0040] Due to the growth of thermophilic Bacillus spores or Bacillus subtilis spores, the pH value will change and the color of the biological indicator will also change. When there is no bacterial growth in the sterilized indicator, the pH value will not change and there will be no color change. Therefore, optionally, the false negative or false positive results can be corrected based on the fluorescence detection combined with the color change in the image of the biological indicator.
[0041] First, the image of the microbial indicator in the microbial indicator test tube is recorded by the image acquisition device, and the image is uploaded to the main control board through the data connection line. Because the color of the biological indicator generally changes from purple to yellow (ethylene oxide changes from green to yellow), the correct color diagram can be pre-stored in the main control board. In step S4, when the image acquisition device uploads the image, the chip will perform a calculation and comparison, and compare it with the color of the preset diagram group, and finally determine whether the color of the biological indicator has changed substantially through the comparison.
[0042] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the utility model should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.
Claims
1. A biometric reader, characterized in that: It comprises a housing, a display module (2), a microorganism indicator test tube (4), an image collector (8), an ultraviolet lamp (16), a fluorescence monitoring template (15) and a main control board (17); the main control board (17) is arranged inside the housing and below the display module (2); The shell comprises an upper shell (3) and a lower shell (18); the display module (2) is fixed to the upper shell (3) via a display module fixing member (1); the microorganism indicator test tube (4) is placed on the upper shell (3) via a channel groove; the image collector (8), the ultraviolet lamp (16), the fluorescence monitoring template (15) and the main control board (17) are arranged in the shell; the image collector (8) is used to record the image of the microorganism indicator in the microorganism indicator test tube (4) and transmit the image to the main control board (17); the ultraviolet lamp (16) is used to emit ultraviolet rays that produce a fluorescence effect with the microorganism indicator; the fluorescence monitoring template (15) is used to receive fluorescence generated by the fluorescence effect and output microorganism concentration data corresponding to the fluorescence to the main control board (17); the main control board (17) determines the sterilization condition based on the received image of the microorganism indicator and the microorganism concentration data.
2. The biometric reader according to claim 1, characterized in that: A first monitoring plate (7) and a second monitoring plate (14) are arranged inside the shell, and the first monitoring plate (7) and the second monitoring plate (14) are respectively located on both sides of the microbial indicator test tube (4).
3. The biometric reader according to claim 2, characterized in that: The image collector (8) is arranged on the first monitoring board (7); The ultraviolet lamp (16) and the fluorescent monitoring template (15) are arranged on the second monitoring board (14).
4. The biometric reader according to claim 2, characterized in that: A heat insulating member (13) is provided on one side of the second monitoring plate (14).
5. The biometric reader according to any one of claims 1 to 4, characterized in that: It also includes a dust cover (5) arranged on the upper shell (3) and used to prevent dust from entering the microbial indicator test tube (4).
6. The biometric reader according to any one of claims 1 to 4, characterized in that: It also includes a mechanical switch (6) arranged on the first monitoring plate (7) for judging whether the microorganism indicator test tube (4) is inserted into the channel slot.
7. The biometric reader according to any one of claims 1 to 4, characterized in that: The front end of the upper housing (3) is also provided with a light-transmitting plate (10) and a status light plate (9) for judging the working state of the biological reader.
8. The biometric reader according to any one of claims 1 to 4, characterized in that: It also comprises a heating body (11), on which a heating plate (12) is arranged for heating the microorganism indicator test tube (4).
Citation Information
Patent Citations
Microbiological detection system and method
CN106841040A
Biological ware and reading ware subassembly read
CN207908511U
And biological reader is used for verifying sterilization effect
CN210465440U