Double-antibody cell strain preservation kit

Abstract

The utility model provides a double-antibody cell strain preservation kit which comprises a reciprocating swing structure, the reciprocating swing structure comprises a swing shaft, the swing shaft is rotatably connected to the inner wall of a shell, one end of the swing shaft is provided with a grip, and the outer wall of the swing shaft is provided with a flat spiral spring body. The reciprocating swing structure is connected with the placing pipe in the kit body, so that in the process of heating the kit filled with the cell strain, the swing shaft is rotated to extrude the flat spiral spring body, and the cell strain is placed in the flat spiral spring body. The flat spiral spring body resets to drive the swing shaft to reset and then rotates by a certain angle in the opposite direction, so that the swing shaft becomes smaller along with deformation and elasticity under the action of the elasticity of the flat spiral spring body, the acceleration is gradually reduced, and finally, due to the existence of inertia, the swing shaft is more stable. After swinging left and right, the swinging shaft gradually reduces swinging and tends to be static, and the cell strain in the swinging shaft swings, so that the cell strain can be uniformly heated.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell line preservation, and specifically relates to a double-antibody cell line preservation kit. Background Art

[0002] Cell strains are cultures with special properties or markers obtained from primary cultures or cell lines by selection or cloning methods.

[0003] The genetic material of the cell line has not changed. When the cells are passed on to the 50th generation, they will be in a "crisis" and can no longer be passed on. However, the genetic material of some cells has changed and has the characteristics of cancer. It is possible to pass on indefinitely under culture conditions. This kind of cell is called a cell line.

[0004] The cell lines are placed inside the test kit for storage. Cell lines that need to be preserved for a long time must be stored in a low-temperature refrigerator at -20℃ to -70℃. When the cell lines are needed, the test kit containing the cell lines needs to be gradually placed in refrigerators of different temperatures for gradual thawing. However, the test kit cannot be shaken during the thawing process, resulting in different cooling speeds of the cell lines in the box. Utility Model Content

[0005] Purpose of the utility model

[0006] In view of the above technical problems, the utility model provides a dual-antibody cell line preservation kit to solve the technical problems mentioned in the background technology.

[0007] Technical Solution

[0008] In order to achieve the above-mentioned purpose, the technical solution provided by the utility model is a dual-antibody cell line preservation kit, comprising a storage box, a cooling bin is provided at the bottom of the inner wall of the storage box, a placement bin is provided at the top of the inner wall of the storage box, a positioning tube is provided between the cooling bin and the placement bin, a rubber sealing ring is provided inside the positioning tube, a shell is provided on the outer wall of the storage box, a reciprocating swinging structure is provided on the outer side of the shell, a kit body is provided inside the placement bin, positioning blocks are provided on both sides of the inner wall of the kit body, a placement tube is provided between the two positioning blocks, connecting shafts are provided at both ends of the placement tube, the placement tube is rotatably connected to the positioning block through the connecting shaft, and card grooves are provided on the outer walls of both sides of the connecting shaft;

[0009] A reciprocating swinging structure includes a swinging shaft, which is rotatably connected to the inner wall of the shell, a handle is provided at one end of the swinging shaft, a planar scroll spring body is provided on the outer wall of the swinging shaft, one end of the planar scroll spring body is connected to the inner wall of the shell, and a fitting groove is provided at the other end of the swinging shaft, and the inner wall of the fitting groove fits with the inner wall of the card slot.

[0010] Preferably, a plurality of storage bins are provided inside the storage box, and the plurality of storage bins are symmetrically distributed on both sides of a horizontal center line of the storage box.

[0011] Preferably, the outer wall of the storage box is provided with a connecting pipe, and the connecting pipe is connected to the cooling bin.

[0012] Preferably, an insertion tube is provided at the bottom of the reagent box body, the interior of the insertion tube is connected to the interior of the reagent box body, and the insertion tube is arranged inside the positioning tube.

[0013] Preferably, a top cover is provided on the top of the placement tube, and the top cover is connected to the placement tube by threads.

[0014] Preferably, a sealing cover is provided on the top of the storage box, connecting blocks are provided on the outer walls of both sides of the storage box, a clamping strip is rotatably connected inside the connecting block, and a planar spiral spring is provided between the clamping strip and the connecting block.

[0015] Beneficial Effects

[0016] Compared with the prior art, the technical solution provided by the utility model has the following beneficial effects:

[0017] The utility model is connected with the placement tube in the reagent box body through the reciprocating swing structure provided. When the reagent box containing the cell strain needs to be heated up, the swing shaft is rotated, the swing shaft squeezes the plane scroll spring body, the plane scroll spring body is reset to drive the swing shaft to reset, and then it is rotated in the opposite direction by a certain angle, so that the swing shaft is affected by the elastic force of the plane scroll spring body, and the swing shaft becomes smaller as the deformation amount and the elastic force become smaller, so the acceleration gradually becomes smaller. Finally, due to the existence of inertia, the swing shaft will swing left and right, and then gradually reduce the swing and tend to be still, so as to swing the cell strain inside, so that the cell strain can be heated evenly. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional diagram of the utility model;

[0019] Figure 2 It is a three-dimensional cross-sectional view of the utility model;

[0020] Figure 3 It is a three-dimensional cross-sectional view of the reagent kit body of the utility model;

[0021] Figure 4 It is a three-dimensional cross-sectional view of the placement tube of the utility model;

[0022] Figure 5 It is a three-dimensional diagram of the swing shaft of the utility model.

[0023] Reference numerals

[0024] 1. Storage box; 2. Cooling chamber; 3. Placement chamber; 4. Connecting tube; 5. Positioning tube; 6. Rubber sealing ring; 7. Test kit body; 8. Insertion tube; 9. Positioning block; 10. Placement tube; 11. Connecting shaft; 12. Card slot; 13. Top cover; 14. Shell; 15. Handle; 16. Swinging shaft; 17. Planar scroll spring body; 18. Fitting groove; 19. Sealing cover; 20. Connecting block; 21. Card strip. DETAILED DESCRIPTION

[0025] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "page", "bottom", "inside", "outside", "clockwise", "counterclockwise", "coaxial", "bottom", "one end", "top", "the other end", "one side", "front", "two ends", "both sides" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation to the present invention.

[0026] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0027] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed", "provided with", etc. should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0028] Reference is now made to the accompanying drawings, in which the purpose of each illustration is only to show certain exemplary embodiments, and is not intended to limit the present invention. In each of the drawings, the same reference numerals represent the same or corresponding parts. The dimensions and proportions in each of the drawings are also only for illustration and should not be interpreted as limiting the present invention. These dimensions may be enlarged relative to the actual product.

[0029] Reference Figure 1-5 , a dual-antibody cell line preservation kit shown in the figure comprises a storage box 1, a cooling bin 2 is provided at the bottom of the inner wall of the storage box 1, a placement bin 3 is provided at the top of the inner wall of the storage box 1, a positioning tube 5 is provided between the cooling bin 2 and the placement bin 3, a rubber sealing ring 6 is provided inside the positioning tube 5, a shell 14 is provided on the outer wall of the storage box 1, a reciprocating swing structure is provided on the outer side of the shell 14, a reagent box body 7 is provided inside the placement bin 3, positioning blocks 9 are provided on both sides of the inner wall of the reagent box body 7, a placement tube 10 is provided between the two positioning blocks 9, connecting shafts 11 are provided at both ends of the placement tube 10, the placement tube 10 is rotatably connected to the positioning block 9 through the connecting shaft 11, and slots 12 are provided on the outer walls of both sides of the connecting shaft 11;

[0030] The reciprocating swinging structure includes a swinging shaft 16, which is rotatably connected to the inner wall of the shell 14, a handle 15 is provided at one end of the swinging shaft 16, a planar spiral spring body 17 is provided on the outer wall of the swinging shaft 16, one end of the planar spiral spring body 17 is connected to the inner wall of the shell 14, and a fitting groove 18 is provided at the other end of the swinging shaft 16, and the inner wall of the fitting groove 18 fits with the inner wall of the slot 12.

[0031] Furthermore, in the above technical solution, a plurality of placement bins 3 are provided inside the storage box 1, and the plurality of placement bins 3 are symmetrically distributed on both sides of the horizontal center line of the storage box 1. The plurality of placement bins 3 can hold a plurality of reagent kit bodies 7, so as to facilitate the storage of the cell strains preserved inside the placement tubes 10 in the reagent kit bodies 7. The dual-antibody cell strain is a branch of the cell strain and the storage method of the cell strain is the same, so it will not be described in detail.

[0032] Furthermore, in the above technical solution, a connecting pipe 4 is provided on the outer wall of the storage box 1 , and the connecting pipe 4 is connected to the cooling bin 2 .

[0033] Furthermore, in the above technical solution, an insertion tube 8 is provided at the bottom of the reagent box body 7, the interior of the insertion tube 8 is connected to the interior of the reagent box body 7, the insertion tube 8 is provided inside the positioning tube 5, and a top cover 13 is provided on the top of the placement tube 10, and the top cover 13 is connected to the placement tube 10 by threads. After the reagent box body 7 is installed into the placement bin 3, the insertion tube 8 is inserted into the interior of the positioning tube 5 and punctures the rubber sealing ring 6, so that cold air can enter the interior of the reagent box body 7 through the insertion tube 8 to preserve the cell line in the placement tube 10 inside the reagent box body 7.

[0034] Furthermore, in the above technical solution, a sealing cover 19 is provided on the top of the storage box 1, and connecting blocks 20 are provided on the outer walls on both sides of the storage box 1. A clamping strip 21 is rotatably connected inside the connecting block 20, and a planar spiral spring is provided between the clamping strip 21 and the connecting block 20. The sealing cover 19 is installed on the top of the storage box 1, and then the planar spiral spring drives the clamping strip 21 to reset, and the clamping strip 21 fits with the top of the sealing cover 19 to fix the sealing cover 19.

[0035] How this utility works:

[0036] Refer to the instruction manual Figure 1-5 First, the cell line is loaded into the placement tube 10 and then the reagent box body 7 is loaded into the placement chamber 3. The sealing cover 19 is installed on the top of the storage box 1. The flat spiral spring drives the card strip 21 to reset and fit with the top of the sealing cover 19 to seal the storage box 1. Then, the storage box 1 is placed in a -20°C to -70°C low-temperature refrigerator to store the cell line. The cold air enters the cooling chamber 2 through the connecting tube 4, and then enters the reagent box body 7 along the insertion tube 8 after cooling, so as to quickly cool the cell line for storage.

[0037] When the cell line needs to be taken out for use, the storage box 1 is placed in a storage bin with a temperature higher than -20°C for gradual cooling. During the cooling process, the swing shaft 16 is manually rotated, and the swing shaft 16 squeezes the plane scroll spring body 17. Then the swing shaft 16 is released, and the plane scroll spring body 17 is reset to drive the swing shaft 16 to rotate. After the swing shaft 16 rotates to the limit position, the swing shaft 16 continues to apply a reverse force to the plane scroll spring body 17, so that the plane scroll spring body 17 is compressed. Repeat the above movement, so that the swing shaft 16 rotates back and forth and drives the placement tube 10 to swing back and forth until it finally stops, and the cell line in the placement tube 10 is swung so that the cell line can be evenly heated.

[0038] The above-described embodiments only express a certain implementation method of the utility model, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the patent of the utility model shall be based on the attached claims.

Claims

1. A dual-antibody cell line preservation kit, characterized in that: include A storage box (1), wherein a cooling chamber (2) is provided at the bottom of the inner wall of the storage box (1), a placement chamber (3) is provided at the top of the inner wall of the storage box (1), a positioning tube (5) is provided between the cooling chamber (2) and the placement chamber (3), a rubber sealing ring (6) is provided inside the positioning tube (5), a shell (14) is provided on the outer wall of the storage box (1), a reciprocating swing structure is provided on the outer side of the shell (14), a reagent box body (7) is provided inside the placement chamber (3), positioning blocks (9) are provided on both sides of the inner wall of the reagent box body (7), a placement tube (10) is provided between the two positioning blocks (9), connecting shafts (11) are provided at both ends of the placement tube (10), the placement tube (10) is rotatably connected to the positioning block (9) via the connecting shaft (11), and slots (12) are provided on the outer walls of both sides of the connecting shaft (11); A reciprocating swing structure comprises a swing shaft (16), wherein the swing shaft (16) is rotatably connected to the inner wall of a shell (14), a handle (15) is provided at one end of the swing shaft (16), a planar spiral spring body (17) is provided on the outer wall of the swing shaft (16), one end of the planar spiral spring body (17) is connected to the inner wall of the shell (14), and a fitting groove (18) is provided at the other end of the swing shaft (16), and the inner wall of the fitting groove (18) fits with the inner wall of the slot (12).

2. A dual-antibody cell line preservation kit according to claim 1, characterized in that: A plurality of storage bins (3) are provided inside the storage box (1), and the plurality of storage bins (3) are symmetrically distributed on both sides of a horizontal center line of the storage box (1).

3. A dual-antibody cell line preservation kit according to claim 1, characterized in that: The outer wall of the storage box (1) is provided with a connecting pipe (4), and the connecting pipe (4) is connected to the cooling bin (2).

4. A dual-antibody cell line preservation kit according to claim 1, characterized in that: An insertion tube (8) is provided at the bottom of the reagent box body (7), the interior of the insertion tube (8) is connected to the interior of the reagent box body (7), and the insertion tube (8) is arranged inside the positioning tube (5).

5. A dual-antibody cell line preservation kit according to claim 1, characterized in that: A top cover (13) is provided on the top of the placement tube (10), and the top cover (13) is connected to the placement tube (10) via threads.

6. A dual-antibody cell line preservation kit according to claim 1, characterized in that: A sealing cover (19) is provided on the top of the storage box (1), and connecting blocks (20) are provided on the outer walls of both sides of the storage box (1). A clamping strip (21) is rotatably connected inside the connecting block (20), and a planar spiral spring is provided between the clamping strip (21) and the connecting block (20).

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